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Source: Cancer Research
Cancer: Adenocarcinoma
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Total 68 results found since Jan 2013.
Abstract 2343: Role of calreticulin in the regulation of MALAT-1 expression in mouse adenocarcinoma cells
Calreticulin (CRT) is a ubiquitously expressed protein, with both calcium binding and chaperone activity. CRT is involved in quality control process during the folding and maturation of proteins in ER. Recently, we showed that overexpression of CRT resulted in the development of metastatic lung adenocarcinoma. In order to examine genes involved in the development of lung cancer we carried out microarray analysis on RNA isolated from mouse lung tumors versus control. In this screen we observed a significant increase in long noncoding RNA (LncRNA) MALAT-1 in the lungs of CRT overexpressing mouse models. MALAT-1 is a LncRNA t...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Massaeli, H., Viswanathan, D., Pillai, D., Mesaeli, N. Tags: Molecular and Cellular Biology Source Type: research
Abstract 2350: Inhibition of Gli1 as a novel therapeutic target for lung squamous cell carcinoma
Conclusions: Our data suggest that Hh pathway downstream factor Gli1 expression is essential for cell proliferation and colony formation in lung SCC. Gli1 is not regulated by canonical Hh pathway regulators, whereas PI3K, ERK, and TGF pathways are involved in its regulation. Blocking Gli1 function is a novel therapeutic strategy for lung SCC treatment.
Citation Format: Chunli Shao. Inhibition of Gli1 as a novel therapeutic target for lung squamous cell carcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Can...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Shao, C. Tags: Molecular and Cellular Biology Source Type: research
Abstract 3903: A sub-set of DCLK1+ve colon cancer stem cells (CSCs) survive curcumin induced autophagy, while co-treatment with curcumin +DCLK1-siRNA eliminates CSCs: Role of long and short isofoms of DCLK1
Conclusion. Our studies strongly suggest that, 1) DCLK1 represents a functional protein for colon cancers, 2) combination of curcumin+DCLK1-siRNA may target and eradicate colon cancer stem cells., and 3) identifying small molecules that inhibit expression of S-isoform may allow to specifically target cancer stem cells, while sparing normal stem cells for cancer treatment purposes.
This work was supported by NIH Granst to PS (R01CA09795909 and RO1CA0975909-S1).
Citation Format: Shubhashish Sarkar, Malaney O'Connell, Carla, Kantara, Pomila Singh. A sub-set of DCLK1+ve colon cancer stem cells (CSCs) survive curcumin induced a...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Sarkar, S., O'Connell, M., Kantara, C., Singh, P. Tags: Tumor Biology Source Type: research
Abstract 3152: Integrin {alpha}6{beta}1 dependent collective cell migration in prostate cancer metastasis
In this study, we optimized the growth and retrieval of tumor cells from 3-dimensional matrigel cultures, to determine the role of integrin α6β1, α6pβ1 and α3β1 in DU145 prostate tumor cell collective migration. The DU145 prostate tumor cells embedded in growth factor reduced matrigel, grew robust networks within 24 hours and contained extensive branching structures. The network formation was shown to be dependent on integrin α6β1 expression as determined by specific knockdown of α6β1 over a 24 hour period using 25nM siRNA targeting. Interestingly, the knockdown of α3β1 integrin had no observable effect on the ...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Rubenstein, C. S., Gard, J., Nagle, R. B., Landowski, T. H., Cress, A. E. Tags: Tumor Biology Source Type: research
Abstract 4455: Targeting a novel kras-integrin-linked kinase regulatory circuitry in pancreatic cancer
In this study, we have identified a novel KRAS-E2F1-ILK-hnRNP A1 regulatory circuitry that governs the expression of oncogenic KRAS. Integrin-linked kinase (ILK) is a serine/threonine kinase that mediates a diversity of cellular functions including cell survival, cell-matrix interactions, angiogenesis and also plays a role in epithelial to mesenchymal transition (EMT) in cancer cells. Dysregulation of ILK expression has been observed in several tumors including breast, ovary, melanoma, lung, prostate and pancreas and reported to be correlated with tumor progression, metastasis and chemoresistance to gemcitabine in pancreat...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Chu, P.-C., Yang, M.-C., Kulp, S. K., Chen, C.-S. Tags: Molecular and Cellular Biology Source Type: research
Abstract 4456: Aurora-A is a downstream target of RAS and forms a positive feedback regulation loop with NF-{kappa}B in non-small cell lung cancer
Previous studies have shown that inhibition of Aurora-A reduces mutant RAS oncogenic activity and that both Aurora-A and RAS activate NF-κB pathway through phosphorylation of IkBa and upregulation of GSKa, respectively. Here we show that Aurora-A expression and kinase activity are regulated by activating mutation of KRAS. Blockage of NF-κB by IκB-S32/36A abrogated KRAS-induced Aurora-A expression and kinase activity. Furthermore, we demonstrated that NF-κB directly bound to Aurora-A promoter and induces Aurora-A transcription. In addition, depletion of Aurora-A by siRNA and pharmacological inhibitor MLN8237 suppresses ...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Kim, D., Kanai, M., Zheng, X., Zheng, D., Coppola, D., Cheng, J. Q. Tags: Molecular and Cellular Biology Source Type: research
Abstract 3485: Efficient transfection of cancer cell lines using the 4D-NucleofectorTM System
In this study we used the 4D-Nucleofector™ System in combination with the 96-well Shuttle™ Add-on for optimizing transfection conditions for multiple cancer cell lines. An exemplary optimization process is depicted for the human prostate carcinoma cell line DU 145 and for the human colorectal adenocarcinoma cell line COLO 205. Optimization steps included the selection of the appropriate Nucleofector™ Solution, Nucleofector™ Program and plasmid DNA concentration.
Transfection parameters were thereby optimized for a variety of adherent and non-adherent human cancer cell lines resulting in transfection efficiencies of...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Schroeder, J., Altrogge, L., Lorbach, E., Kokatam, S., Schaepermeier, S., Weigel, M., Andretta-Beu, G., Buesch, S., Grabeck, T., Krumnow, A., Spicker, S., Kallol, S., Kapoor, P., Toell, A. Tags: Molecular and Cellular Biology Source Type: research
Abstract 2485: Secretome signature associated with TITF1/NKX2-1-negative non-small cell lung cancer
Conclusion: Our findings suggest functional relevance of SRGN in tumor microenvironment and tumor aggressiveness in TITF1/NKX2-1-negative NSCLC. Validation study for plasma levels of CXCL1 and CXCL5 in NSCLC subjects is now on-going.
Citation Format: Ayumu Taguchi, Muge Celiktas, Dhillon Dilsher, Qing Zhang, Chee-Hong Wong, Alice Chin, Adi Gazdar, Samir Hanash. Secretome signature associated with TITF1/NKX2-1-negative non-small cell lung cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Taguchi, A., Celiktas, M., Dilsher, D., Zhang, Q., Wong, C.-H., Chin, A., Gazdar, A., Hanash, S. Tags: Cancer Chemistry Source Type: research
Abstract 4026: Attenuation of pancreatic cancer cell migration and invasion through a targeted inhibition of the Rac GEF Vav1
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal forms of human cancer, due in large part to a high incidence of metastasis and resistance to conventional chemotherapies. As such, new therapies are needed to halt the metastatic process. The proto-oncogene Vav1 is ectopically expressed in over half of human pancreatic cancers, and its expression correlates with a poor prognosis in humans and promotes survival and transformation in isolated tumor cells. We have found that Vav1, through its GEF activity towards Rac1, also potently induces migration by pancreatic tumor cells. In addition, Vav1 also signals thr...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Razidlo, G. L., Magnine, C., Sletten, A. C., Hurley, R. M., McNiven, M. A. Tags: Tumor Biology Source Type: research
Abstract 3526: The mTORC2 component RICTOR plays a key role in lung cancer cell growth
RICTOR (Rapamycin-insensitive companion of mTOR protein) is a key component of mTORC2 (the mammalian target of rapamycin complex 2). One of the most-recognized targets for RICTOR-mTORC2 is AKT (Ser473). RICTOR also carries functions independent of mTORC2. RICTOR signaling has been suggested to play key roles in regulating cancer cell migration, invasion and metastasis in breast, ovarian, colorectal cancers and gliomas. The potential roles of RICTOR in lung cancer remain to be elucidated. We first examined the expression profile of RICTOR in primary lung tumor specimens and in lung cancer cell lines by immunohistochemistry....
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Cheng, H., Zou, Y., Borczuk, A., Qiu, W., Piperdi, B., Kim, M., Halmos, B., Perez–Soler , R. Tags: Molecular and Cellular Biology Source Type: research
Abstract 4054: Elongation factor-2 kinase (eEF-2K) promotes cell invasion and epithelial mesenchymal transition through regulation of TG2-mediated signaling in human pancreatic cancer cells
Pancreatic ductal adenocarcinoma (PDAC) accounts for the majority of pancreatic cancer (PaCa) cases and is currently uncurable with poor prognosis/survival rates (∼4% 5-year survival). The extensive local tumor invasion, early metastasis/systemic dissemination and resistance to existing cancer therapies are the major characteristics of PaCa and the impediment to effective cure of this disease. Although PaCa has a well-defined spectrum of highly oncogenic lesions (e.g, mutations in K-RAS, p53, p16ink4a, and SMAD4/DPC4), effective therapies have not yet been developed. Therefore, novel molecular targets-based therapeutic s...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Ashour, A. A., Alpay, S. N., Gurbuz, N., Abdel-Aziz, A.-A. H., Mansour, A. M., Ozpolat, B. Tags: Tumor Biology Source Type: research
Abstract 2273: c-Rel is a critical mediator of NF-{kappa}B-dependent apoptosis resistance of pancreatic cancer cells against TRAIL
In conclusion, c-Rel is a critical mediator of NF-κB dependent anti-apoptotic signalling in PDAC through activation of NFATc2 and COX-2.
Citation Format: Claudia Geismann, Frauke Grohmann, Gabriele Wirths, Susanne Sebens, Anita Dreher, Robert Häsler, Sebastian Zeissig, Stefan Schreiber, Philip Rosenstiel, Heiner Schäfer, Alexander Arlt. c-Rel is a critical mediator of NF-κB-dependent apoptosis resistance of pancreatic cancer cells against TRAIL. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;7...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Geismann, C., Grohmann, F., Wirths, G., Sebens, S., Dreher, A., Hasler, R., Zeissig, S., Schreiber, S., Rosenstiel, P., Schafer, H., Arlt, A. Tags: Molecular and Cellular Biology Source Type: research
Abstract 3318: Using synthetic lethal screening to identify therapeutic targets for innately platinum resistant lung cancer
Although platinum-based chemotherapy is the standard of care for most cases of advanced lung adenocarcinoma, its effectiveness is limited by the frequent incidence of innate chemoresistance. As a result, response rates rarely exceed 20%, even though cis-platinum and carboplatin are highly effective in other settings such as small cell lung, ovarian and testicular cancers. We hypothesized that innate chemoresistance in lung adenocarcinoma is mediated by one or more signalling pathways dependent on the expression of a single gene, and that these pathways could ultimately be targeted therapeutically. To address this question,...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Marini, K. D., Rossello, F. J., Martelotto, L. G., Watkins, D. N. Tags: Molecular and Cellular Biology Source Type: research
Abstract 2330: Transcription factor MAZ promotes cell growth and aggressive behavior of human pancreatic cancer cells
Pancreatic ductal adenocarcinoma (PDAC) is the fourth most common cancer in the United States and the eighth worldwide. PDAC is most aggressive type of cancer that spreads rapidly and is seldom detected in its early stages as signs and symptoms may not appear until pancreatic cancer is quite advanced. MAZ (Myc-associated zinc-finger protein) or SAF1 (Serum amyloid A activating factor 1) gene is a member of multiple Cys2-His2-type zinc finger proteins that are activated in response to various inflammatory signals and may act as a transcription factor with dual roles in transcription initiation and termination. Deregulation ...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Maity, G., Sarkar, S., Dhar, K., Dhar, G., Haque, I., Banerjee, S. K., Banerjee, S. Tags: Molecular and Cellular Biology Source Type: research
Abstract 3355: Inhibiting glyoxylase 1 as a strategy to target highly glycolytic cancer cells
Cancer cells typically display altered glucose metabolism characterized by a preference of aerobic glycolysis (Warburg effect) resulting in higher levels of glycolytic waste including methylglyoxal (MG). GLO1 (Glyoxalase 1) functions in the detoxification of MG: MG reacts with glutathione to form hemithioacetal, which is converted into S-D-Lactoylglutathione by GLO1 and further metabolised into D-lactate by GLO2. When not detoxified, MG acts as a cytotoxic reagent by forming DNA- and protein-adducts (advanced glycation end products = AGEs) that subsequently lead to cell death. Therefore, GLO1 has been discussed as a potent...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Gruenewald, S., Steckel, M., Timmermann, A., Rehwinkel, H., Steigemann, P., Zacharias, S., Walter, A., Bauser, M., Haegebarth, A. Tags: Molecular and Cellular Biology Source Type: research
