Filtered By:
Source: Cancer Research
Cancer: Adenocarcinoma
This page shows you your search results in order of date. This is page number 3.
Order by Relevance | Date
Total 68 results found since Jan 2013.
Abstract A112: Eradication of cancer stem-like cells in PDAC
Conclusion: These data indicate that p21 maintains chemotherapy induced CSLCs quiescence and drug resistance such that targeting p21 in combination with cytotoxic therapies is beneficial in eradicating both basal and Gem transformed CSLCs.Note: This abstract was not presented at the conference.Citation Format: Thiruvengadam Arumugam, Vijaya Ramachandran, Craig Logsdon. Eradication of cancer stem-like cells in PDAC. [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer: Innovations in Research and Treatment; May 18-21, 2014; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2015;75(13 Suppl):Abstract nr A112.
Source: Cancer Research - June 30, 2015 Category: Cancer & Oncology Authors: Arumugam, T., Ramachandran, V., Logsdon, C. Tags: New Therapies Source Type: research
Abstract B114: c-Rel is a critical mediator of NF-{kappa}B dependent TRAIL resistance of pancreatic cancer cells
In conclusion, we were able to delineate a novel c-Rel, NFATc2 and COX-2 dependent anti-apoptotic signalling pathway in PDAC with broad clinical implications for pharmaceutical intervention strategies.Citation Format: Claudia Geismann, Frauke Grohmann, Robert Häsler, Philip Rosenstiel, Günter Schneider, Sebastian Zeissig, Stefan Schreiber, Heiner Schäfer, Alexander Arlt. c-Rel is a critical mediator of NF-κB dependent TRAIL resistance of pancreatic cancer cells. [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer: Innovations in Research and Treatment; May 18-21, 2014; New Orleans, LA. Phila...
Source: Cancer Research - June 30, 2015 Category: Cancer & Oncology Authors: Geismann, C., Grohmann, F., Hasler, R., Rosenstiel, P., Schneider, G., Zeissig, S., Schreiber, S., Schafer, H., Arlt, A. Tags: Other Topics Source Type: research
Abstract A13: Mechanisms of E47 induced quiescence and acinar cell differentiation in human pancreatic cancer cells
Pancreatic ductal adenocarcinoma (PDA) initiates from quiescent acinar cells that attain a Kras mutation, undergo acinar-ductal metaplasia and rapidly acquire increased growth potential. During this process several transcription factors from the basic helix-loop-helix (bHLH) family are downregulated while expression of their inhibitor Id3 is induced. Previously we showed that Id3 knockdown with siRNA resulted in growth arrest in PDA cells. Here we queried whether aggressive PDA cells can be reprogrammed to revert to their original quiescent acinar cell phenotype by shifting bHLH transcription programs. In order to mitigate...
Source: Cancer Research - June 30, 2015 Category: Cancer & Oncology Authors: Kim, S., Yang, C., Riha, C., Lamy, R., Jakubison, B. L., Konieczny, S. F., Itkin-Ansari, P. Tags: Development Source Type: research
Abstract A39: The activation of {beta}1-integrin by type i collagen coupling with the Hedgehog pathway promotes the epithelial-mesenchymal transition in pancreatic cancer cells
In this study, we demonstrated that pancreatic cancer cells exhibited increased proliferation and decreased apoptosis in response to type I collagen. In addition, exposed to type I collagen, PDAC cells lost the expression of E-cadherin and increased expression of mesenchymal markers, including N-cadherin and vimentin, which was correlated with enhanced cell migration and invasiveness. Conversely, the knockdown of β1-integrin abolished the effects induced by type I collagen. Further investigation revealed that type I collagen activates β1-integrin accompanied with significant up-regulation of Gli-1. siRNA specific to Gli-...
Source: Cancer Research - June 30, 2015 Category: Cancer & Oncology Authors: Duan, W., Ma, Q., Ma, J., Xu, Q., Lei, J., Wu, E. Tags: Development Source Type: research
Abstract A71: Post-transcriptional regulation of the proto-oncogene PIM1 by the mRNA stability factor HuR: implications for pancreatic cancer therapeutic response and cell survival
Pancreatic ductal adenocarcinoma (PDA) is an aggressive malignancy characterized by its insidious development and resistance to conventional and targeted therapies. As a result of selective pressures imposed by cytotoxic agents and the tumor microenvironment, PDA cells orchestrate a potent and elusive chemoresistant mechanism. Recently, the serine-threonine kinase PIM1 has emerged as a key regulator of PDA cell survival under cancer-associated stress (e.g: cytotoxic DNA-damaging agents, hypoxia). However, the molecular mechanism behind PIM1 overexpression in PDAs is unknown. Here, we demonstrate that cis-acting AU-rich ele...
Source: Cancer Research - June 30, 2015 Category: Cancer & Oncology Authors: Blanco, F. F., Meisner-Kober, N., Londin, E., Rigoutsos, I., Winter, J., Yeo, C., Brody, J. Tags: Heterogeneity in Tumor Progression Source Type: research
Abstract 22: RECQ1 promotes the expression of genes significantly associated with cancer progression
RecQ helicases (represented by five homologs in humans: RECQ1, WRN, BLM, RECQ4, and RECQ5) are vital to maintain genomic stability under replication stress and germ line mutations in WRN, BLM and RECQ4 are associated with cancer predisposition syndromes. Expression of RECQ1 has been associated with the process of cellular transformation and over-expression of RECQ1 has been reported in a variety of human cancers. Furthermore, siRNA-mediated knockdown of RECQ1 has a cancer cell specific effect in cell culture models and also suppresses tumor growth in mouse xenografts including lung, liver, pancreatic and colorectal cancer....
Source: Cancer Research - December 12, 2014 Category: Cancer & Oncology Authors: Lu, X., Li, X. L., Parvathaneni, S., Lal, A., Sharma, S. Tags: Poster Presentations Source Type: research
Abstract 1126: Tristetraprolin inhibits Twist1-induced cancer cell migration
This study demonstrates that TTP plays a critical role in EMT process. Overexpression of TTP in ovarian cancer cells decreased the expression of mesenchymal markers (N-cadherin and Vimentin), increased the expression of epithelial marker (E-cadherin), and inhibited cell migration and invasion activities. On the contrary, inhibition of TTP by siRNA decreased the expression of E-cadherin, increased the expression of N-cadherin and Vimentin, and promoted cell migration and invasion. The cDNA microarray analysis revealed that Twist1 was significantly down-regulated in TTP-overexpressed cells. Twist1 mRNA contains ARE within it...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Cho, W. J., Yoon, N. A., Vo, M.-T., Min, Y. J., Park, J. W. Tags: Tumor Biology Source Type: research
Abstract 133: Role of Glypican-3 (GPC3) on tumor progression of the human mammary gland
We have previously shown that the reexpression of Glypican-3 (GPC3), a proteoglycan downregulated in breast cancer, leads to the impairment of the in vivo metastatic capacity of the murine LM3 mammary adenocarcinoma cells. On the basis of clinical and translational potential of GPC3, the aim of this work was to assess whether GPC3 acts as a metastasis suppressor in human cells. So, we generated a preclinical breast cancer cell model. We chose MCF-7 cell line (poorly-metastatic, GPC3 +) and MDA-MB231 cell line (metastatic, GPC3 -) to be genetically modified.
GPC3 expression was blocked in MCF-7 cells by siRNA. We demonstrat...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Castillo, L. F., Tascon, R. S., Joffe, E. B. d. K., Peters, M. G. Tags: Tumor Biology Source Type: research
Abstract 1131: Snail- and ERK2-dependent signaling enhances breast cancer cell resistance to hydroxytamoxifen
Snail transcription factor and MAPK/ERK signaling regulate EMT and chemotherapy resistance in various tumor models by binding to target promoters (i.e., E-cadherin, maspin, ER-α). ERK1 is expressed during embryogenesis and in non-metastatic cells; ERK2 is implicated during vasculogenesis and promotes stem cell phenotype in triple negative breast cancer. Nuclear-localized ERK is associated with more active and potentially metastatic breast and ovarian carcinoma cells; cytoplasmic-localized ERK is a good prognostic factor. The role that Snail plays during the transition from cytoplasmic ERK1 to nuclear ERK2 has not been inv...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Smith, B. N., Nagappan, P., Taliaferro-Smith, L., Mezencev, R., Yates, C., Hinton, C., Odero-Marah, V. Tags: Tumor Biology Source Type: research
Abstract 1155: Chemoresistance acquisition by ovarian adenocarcinoma cells due to microenvironment
Epithelial Ovarian Carcinoma is characterized by high frequency of recurrence (70% of patients) and carboplatin resistance acquisition. We recently showed that Carcinoma-Associated-Mesenchymal Stem Cells (CA-MSC) are involved in ovarian tumor growth via the facilitation of angiogenesis in the tumor site as well as in ovarian cancer chemoresistance acquisition. Our aim is to identify the mechanisms by which CA-MSC activate tumor cells signaling pathway for both effects. First we showed that factors released by CA-MSC are able to induce angiogenic cytokines (IL-6, IL-8 and VEGF) synthesis by tumor cells in a cell line specif...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Thibault, B., Castells, M., Mihas, D., Genre, L., Gandy, C., Mery, E., Delord, J. P., Couderc, B. C. Tags: Tumor Biology Source Type: research
Abstract 26: MUC1 enhances neuropilin-1 signaling in pancreatic ductal adenocarcinoma
Pancreatic ductal adenocarcinoma (PDA) has the worst prognosis of all cancers and is the 4th leading cause of cancer-related deaths in the United States. Mucin1 (MUC1) is a transmembrane glycoprotein over-expressed in more than 60% of PDA and its expression correlates with high metastases and poor prognosis. In PDA, there is a correlation between blood vessel density, tumor levels of VEGF, and disease progression. We have recently discovered a novel association between MUC1 and neuropilin-1 (NRP-1) expression. Neuropilin-1 (NRP-1) is a co-receptor for VEGF165 and blockade of NRP-1-VEGF165 interaction has been shown to inhi...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Zhou, R., Curry, J., Grover, P., Roy, L. D., Leung, T., Mukherjee, P. Tags: Tumor Biology Source Type: research
Abstract LB-48: DCLK1 targeted monoclonal antibodies demonstrate therapeutic potential against pancreatic ductal adenocarcinoma
In this study we evaluated whether DCLK1-targeted monoclonal antibodies (mAbs) demonstrate therapeutic activity against pancreatic cancer. Human pancreatic cancer cells (AsPC-1) were treated with mAbs against the C-terminal domain of human DCLK1 to assess their effect on gene expression, cell proliferation, migration, invasion, and tumorigenicity. Gene expression levels were analyzed by quantitative real-time RT-PCR. The proliferative and invasive potential of cells with or without mAb treatment were compared using a MTT assay for proliferation, wound healing assay for migration, and Matrigel coated transwell assay for inv...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Qu, D., Weygant, N., May, R., Chandrakesan, P., Owen, D., Sureban, S., Houchen, C. Tags: Tumor Biology Source Type: research
Abstract 2244A: Prion protein cross-talks with Notch1 to promote pancreatic ductal adenocarcinoma progression
In this study, we would like to investigate whether PrP also involves other signal transduction pathway in PDACs, especially Notch pathway.
Using immunoprecipitation and immunocytometry, we investigated the binding partners for PrP in several PDAC cell lines and found that PrP associates with Notch1 but not with Notch2 or Notch3 in cytoplasm and cell membrane. The association of PrP and Notch1 was further validated by confocal microscopy. Silencing PrP by siRNA not only decreased PrP expression, but also decreased Notch1 in the cytoplasm and the nuclear localized cleaved Notch1. Knocking down PrP in PDAC cells with high le...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Wang, Y., Huang, D., Zhou, L., Xin, W. Tags: Molecular and Cellular Biology Source Type: research
Abstract 471: Transcriptional coactivator CBP regulates hTERT expression through the cooperation with Sp1/AP-2{beta} and predicts poor prognosis in human lung cancers
In this study, we identified CBP as a transcriptional coactivator that specifically binds to hTERT promoter through co-anchoring with transcriptional factors Sp1 and AP-2β and promotes hTERT expression in human lung cancer cells. Streptavidin-agarose pulldown and chromatin immunoprecipitation assays revealed the tumor-specific binding of CBP, Sp1 and AP-2β on the hTERT promoter. The colocalization and interaction between CBP and Sp1 or AP-2β in lung cancer cells were detected. High expression levels of CBP and hTERT in lung cancer cells and tissues were also detected compared to normal lung cells and adjacent normal lun...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Guo, W. Tags: Molecular and Cellular Biology Source Type: research
Abstract 764: A role for GLI1 in the development of multidrug resistance in rhabdomyosarcoma (RMS) cells
RMS is the most common sarcoma of childhood. About 30% of patients with localized tumors will recur following treatment. The outcome for patients with recurrent RMS remains poor. Therefore, development of chemotherapy resistance during RMS therapy represents an important problem and novel approaches to prevent or reverse drug resistance are essential. Activation of multidrug transporter genes, including MDR1, MRP1, LRP and TAP1 represents an important mechanism for drug resistance in RMS. However, the mechanism of expression of multidrug resistance genes in RMS is incompletely understood. Recent reports have suggested a ro...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Yoon, J. W., Lamm, M., Leong, K.-F., Iannaccone, S., Iannaccone, P., Walterhouse, D. Tags: Experimental and Molecular Therapeutics Source Type: research
