Serum HE4 superior to CA125 in predicting poorer surgical outcome of epithelial ovarian cancer
AbstractEpithelial ovarian cancer (EOC) remains the deadliest form of gynecological cancers. Optimal tumor debulking, no matter the primary or the interval, is the most important prognostic factor for EOC, so there is an urgent demand for biomarkers to predict surgical outcome. The aim of this study was to investigate whether serum human epididymis protein 4 (HE4) and cancer antigen 125 (CA125) could predict surgical outcome of EOC. The levels of preoperative serum HE4 and CA125 were determined by electrochemiluminescence (ECLIA) in 82 EOC patients, comprising 39 subjected to primary debulking surgery (PDS) and 43 with ext...
Source: Tumor Biology - September 14, 2016 Category: Cancer & Oncology Source Type: research
Transcriptome sequencing of HER2-positive breast cancer stem cells identifies potential prognostic marker
In conclusion, our results suggest that CA12 may be associated with BCSCs, especially HER2-positive BCSCs, and is a potential novel therapeutic target and biomarker. (Source: Tumor Biology)
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
Emerging tale of UPR and cancer: an essentiality for malignancy
AbstractA set of cellular response to counter any alteration in homeostasis of a cell originating at endoplasmic reticulum is collectively termed as unfolded protein response (UPR). It initially is adaptive in nature as to restore cellular normalcy failing in course often activates pro-apoptotic signaling pathway resulting in cell death. UPR has emerged as an essential adaptation mechanism that cross talk with various cellular processes for cancer pathogenesis. Interestingly, it plays diverse role in plethora of signaling pathways instrumental in transformation, cell invasion, cell migration, metastasis, neovascularization...
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
TRPM7 channel inhibition mediates midazolam-induced proliferation loss in human malignant glioma
AbstractThe melastatin-like transient receptor potential 7 (TRPM7) has been implicated in proliferation or apoptosis of some cancers, indicating the potential of TRPM7 as an anti-anaplastic target. Here, we identified the characteristic TRPM7 channel currents in human malignant glioma MGR2 cells, which could be blocked by a pharmacologic inhibitor Gd3+. We mined the clinical sample data from Oncomine Database and found that human malignant glioma tissues expressed higher TRPM7 mRNA than normal brain ones. Importantly, we identified a widely used clinical anesthetic midazolam as a TRPM7 inhibitor. Midazolam treatment for se...
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
Global methylation profiling to identify epigenetic signature of gallbladder cancer and gallstone disease
AbstractPromoter methylation in various tumor suppressor genes is reported to influence gallbladder carcinogenesis. Here, we aimed to identify methylation status in gallbladder cancer (GBC) by performing a comprehensive genome-wide DNA methylation profiling. The methylation status of 485,577 CpG sites were investigated using Illumina ’s Infinium Human Methylation 450 BeadChip array in 24 tissues (eight each of tumor, adjacent non-tumor, and gallstone). About 33,443 differentially methylated sites (DMRs) were obtained in the whole human genome, of which 24,188 (72 %) were hypermethylated and 9255 (28 %) were hypomethyla...
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
Tumour biology of obesity-related cancers: understanding the molecular concept for better diagnosis and treatment
AbstractObesity continues to be a major global problem. Various cancers are related to obesity and proper understanding of their aetiology, especially their molecular tumour biology is important for early diagnosis and better treatment. Genes play an important role in the development of obesity. Few genes such as leptin, leptin receptor encoded by thedb (diabetes), pro-opiomelanocortin, AgRP and NPY and melanocortin-4 receptors and insulin-induced gene 2 were linked to obesity. MicroRNAs control gene expression via mRNA degradation and protein translation inhibition and influence cell differentiation, cell growth and cell ...
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
Hint1 suppresses migration and invasion of hepatocellular carcinoma cells in vitro by modulating girdin activity
AbstractHistidine triad nucleotide-binding protein 1 (Hint1) is a haploinsufficient tumor suppressor gene. Its role in cancer cell migration has not been previously speculated. In the current study, we examined the expression of Hint1 in metastatic and non-metastatic lymph nodes of hepatocellular carcinoma (HCC) patients and further elucidated the effect of Hint1 expression on girdin expression and phosphorylation of AKT and ERK1/2 and on the migration of HCC cells in vitro. Expression of Hint1 and girdin in primary HCC tissues and metastatic and non-metastatic lymph nodes was determined by RT-PCR assays. HepG2 cells were ...
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
IL-13 receptor α2 stimulates human glioma cell growth and metastasis through the Src/PI3K/Akt/mTOR signaling pathway
In conclusion, our results suggest that the high expression of IL-13Rα2 is significantly associated with the growth and metastasi s of human glioma cells via the Scr/PI3K/Akt/mTOR pathway, while IL-13Rα2 may be a potential therapeutic target for glioma treatment. (Source: Tumor Biology)
Source: Tumor Biology - September 13, 2016 Category: Cancer & Oncology Source Type: research
Determination of a CD4 + CD25 − FoxP3 + T cells subset in tumor-draining lymph nodes of colorectal cancer secreting IL-2 and IFN-γ
AbstractCD4+CD25−FoxP3+ cells are a newly recognized subset of T cells which was first reported in autoimmune diseases. In our previous study, this subset was detected in tumor-draining lymph nodes (TDLNs) of patients with breast cancer. As little is known about their function in TDLNs of cancer patients, in this study, their frequency as well as their ability to produce interleukin (IL)-2, IL-10, or interferon (IFN)- γ were investigated in TDLNs of colorectal cancer (CRC) patients. Mononuclear cells were isolated from lymph nodes of 13 patients with CRC using Ficoll-Hypaque gradient centrifugation. Cells were stimulate...
Source: Tumor Biology - September 12, 2016 Category: Cancer & Oncology Source Type: research
IGF-I receptor as an emerging potential molecular-targeted for hepatocellular carcinoma in vitro and in vivo
In this study, IGF-IR expression among different HCC cell lines and silencing its gene transcription on effects of HCC were investigated by short hairpin RNA (shRNA). Specific shRNA was successfully transfected into Bel-7404 or PLC/PRF/5 cells with 90 or 71 % efficiency. The inhibiting rate of IGF-IR at mRNA level were 54.9 % in Bel-7404 or 59.6 % in PLC/PRF/5 cells in accordance with its protein suppression, with the cell cycles at the G1 phase arrest and decreasing cyclinD1 via promoting apoptosis in vitro. With the xenograft models of PLC/PRF/5 cells inserted specific shRNA in vivo, the tumor-forming time (14.0 ±...
Source: Tumor Biology - September 12, 2016 Category: Cancer & Oncology Source Type: research
Aberrant microRNA expression in tumor mycosis fungoides
AbstractHerein, miRNA candidates relevant to mycosis fungoides were investigated to provide data on the molecular mechanisms underlying the pathogenesis of the disease. The miRNA expression profile of skin biopsies from patients with tumor stage MF (tMF) and normal donors was compared using miRNA microarrays. Overall, 154 miRNAs were found differentially expressed between tMF and the control cohort with the majority of them being up-regulated (57 %). Among the upregulated miRNAs, miR-3177, miR-514b-3p, miR-1267, and miR-1282 were exclusively detected in 70 % of tMF. Additional upregulated miRNAs included miR-34a, miR-29...
Source: Tumor Biology - September 12, 2016 Category: Cancer & Oncology Source Type: research
Bone marrow-derived mesenchymal stem cells increase drug resistance in CD133-expressing gastric cancer cells by regulating the PI3K/AKT pathway
In conclusion, we demonstrated that BM-MSCs increased the anti-apoptotic abilities and drug resistance of CD133-expressing cells via activation of the PI3K/Akt pathway following Bcl-2 upregulation and BAX downregulation, in which CD133 played a significant role. Targeting this route may help improve the efficacy of chemotherapy in gastric cancer. (Source: Tumor Biology)
Source: Tumor Biology - September 11, 2016 Category: Cancer & Oncology Source Type: research
MiR-429 is linked to metastasis and poor prognosis in renal cell carcinoma by affecting epithelial-mesenchymal transition
AbstractMicroRNAs (miRNAs) have been proven to be important oncogenes and tumor suppressors in wide range of cancers, including renal cell carcinoma (RCC). In our study, we evaluated miRNA-429 as potential diagnostic/prognostic biomarker in 172 clear cell RCC patients and as a potential regulator of epithelial-mesenchymal transition (EMT) in vitro. We demonstrated that miR-429 is down-regulated in tumor tissue samples (P < 0.0001) and is significantly associated with cancer metastasis (P < 0.0001), shorter disease-free (P = 0.0105), and overall survival (P = 0.0020). In addition, ectopic expression of miR-4...
Source: Tumor Biology - September 11, 2016 Category: Cancer & Oncology Source Type: research
High expression of TRIM44 is associated with enhanced cell proliferation, migration, invasion, and resistance to doxorubicin in hepatocellular carcinoma
In conclusion, our results suggest that TRIM44 may be a novel prognostic indicator and potential therapeutic target of HCC. (Source: Tumor Biology)
Source: Tumor Biology - September 11, 2016 Category: Cancer & Oncology Source Type: research
U/G SNP rs111904020 in 3 ′UTR of STAT3 regulated by miR-214 promotes hepatocellular carcinoma development in Chinese population
In conclusion, our findings have shown that the SNP rs111904020 (U/G) in STAT3 3′UTR acted as promotion factors in the HCC developm ent through disrupting the regulatory role of miR-214 in STAT3 expression. (Source: Tumor Biology)
Source: Tumor Biology - September 11, 2016 Category: Cancer & Oncology Source Type: research
