The Journal of Steroid Biochemistry and Molecular Biology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Development and application of a LC-MS/MS assay for simultaneous analysis of 25-hydroxyvitamin-D and 3-epi-25-hydroxyvitamin-D metabolites in canine serum
We describe a simplified workflow utilising supported liquid extraction (SLE) without derivatization that provides good linearity (mean r> 0.996) and accuracy across a broad dynamic range of 4 - 500 nmol/L for D3 metabolites and 7.8 – 500 nmol/L for D2 metabolites. Upon application of this assay to 117 canine serum samples, 25(OH)D3 was detectable in all samples with a median concentration of 82.1 nmol/L (inter-quartile range (IQR) 59.7 – 101.8 nmol/L). 3-epi-25(OH)D3 could be detected in 87.2% of the study population, with a median concentration of 5.2 nmol/L (2.4 – 8.1 nmol/L). However, 3-epi-25(OH)D3 w...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 17, 2020 Category: Biochemistry Source Type: research

Impact of Maternal Smoking Associated Lyso-phosphatidylcholine 20:3 on Offspring Brain Development
In conclusion, maternal smoking during pregnancy impacted on male offspring’s development including brain development. The smoking related metabolite lyso-phosphatidylcholine 20:3 was associated with head circumference of male offspring. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 16, 2020 Category: Biochemistry Source Type: research

Synthesis and application of steroidal 22,16β-carbolactones: a review
Publication date: Available online 14 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Izabella JastrzebskaGraphical abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 15, 2020 Category: Biochemistry Source Type: research

Vitamin D and sex steroid production in men with normal or impaired Leydig cell function
In conclusion, vitamin D deficiency is associated with lower testosterone/estradiol ratio in young men and lower Leydig cell sensitivity after hCG-stimulation in men with impaired gonadal function. The significant effect of 1,25(OH)2D3 on testosterone production in a human testis model supports that the stimulatory effect at least in part may be direct. Larger placebo-controlled studies are needed to determine whether vitamin D supplementation can influence testosterone production. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 15, 2020 Category: Biochemistry Source Type: research

25(OH)D3 stimulates the expression of vitamin D target genes in renal tubular cells when Cyp27b1 is abrogated
Publication date: Available online 13 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Takahiro Kikuyama, Takao Susa, Mimi Tamamori-Adachi, Masayoshi Iizuka, Miho Akimoto, Hiroko Okinaga, Yoshihide Fujigaki, Shunya Uchida, Shigeru Shibata, Tomoki OkazakiAbstractRecently, it was reported that 25(OH)D3 (25D3) has physiological bioactivity in certain tissues derived from Cyp27b1 knockout mice. To investigate the function of 25D3 in the kidney as an informational crossroad of various calciotropic substances, we employed the CRISPR-Cas9 system to knock out Cyp27b1 in the mouse renal distal...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 14, 2020 Category: Biochemistry Source Type: research

Cholestenoic Acid Analogues as Inverse Agonists of the Liver X Receptors
Publication date: Available online 10 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lautaro D. Alvarez, María V. Dansey, María F. Ogara, Carina I. Peña, René Houtman, Adriana S. Veleiro, Adali Pecci, Gerardo BurtonAbstractLiver X Receptors (LXRs) are ligand dependent transcription factors activated by oxidized cholesterol metabolites (oxysterols) that play fundamental roles in the transcriptional control of lipid metabolism, cholesterol transport and modulation of inflammatory responses. In the last decade, LXRs have become attractive pharmacological targets for intervention in...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 11, 2020 Category: Biochemistry Source Type: research

Human dehydrogenase/reductase SDR family member 11 (DHRS11) and aldo-keto reductase 1C isoforms in comparison: Substrate and reaction specificity in the reduction of 11-keto-C19-steroids
Publication date: Available online 8 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Satoshi Endo, Yoshifumi Morikawa, Yudai Kudo, Koichi Suenami, Toshiyuki Matsunaga, Akira Ikari, Akira HaraAbstractRecent studies have shown that an adrenal steroid 11β-hydroxy-4-androstene-3,17-dione serves as the precursor to androgens, 11-ketotestosterone and 11-ketodihydrotestosterone (11KDHT). The biosynthetic pathways include the reduction of 3- and 17-keto groups of the androgen precursors 11-keto-C19-steroids, which has been reported to be mediated by three human enzymes; aldo-keto reductase ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 9, 2020 Category: Biochemistry Source Type: research

Chemotherapy and vitamin D supplement use are determinants of serum 25-hydroxyvitamin D levels during the first six months after colorectal cancer diagnosis
In conclusion, vitamin D supplement use and treatment appear to be important determinants of 25(OH)D3 levels during the first six months after CRC diagnosis, although the difference in 25(OH)D3 levels was minor.ClinicalTrials.gov Identifier: NCT03191110 (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 8, 2020 Category: Biochemistry Source Type: research

Investigating a suitable model for the study of vitamin D mediated regulation of human placental gene expression
This study compares the expression of amino acid transporters and vitamin D related genes in human term placenta with the BeWo and human embryonic kidney (HEK)293 cell lines. HEK293 cells, as transporting epithelium may be more similar to placenta. Gene expression in term placenta was much more similar to HEK293 than BeWo. This study provides further evidence that the BeWo cell line is not an appropriate model for villous trophoblast and a model that more closely represents the human placenta is now required to investigate the effects of vitamin D on the placenta ex-vivo. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 3, 2020 Category: Biochemistry Source Type: research

The role of diosgenin in diabetes and diabetic complications
This article reviews the effects of diosgenin through multiple targets and multiple pathways in diabetes and its complications which including diabetic nephropathy, diabetic liver disease, diabetic neuropathy, diabetic vascular disease, diabetic cardiomyopathy, diabetic reproductive dysfunction, and diabetic eye disease. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 31, 2019 Category: Biochemistry Source Type: research

Association of nonalcoholic fatty liver disease with serum vitamin D levels in combination of physical fitness in Korean older adults
Publication date: Available online 28 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Inhwan Lee, Eunmi Park, Jinkyung ChoAbstractThe prevalence of non-alcoholic fatty liver disease (NAFLD) tends to increase with age, but little is known regarding relations between the risk of NAFLD in older adults and serum vitamin D (25-hydroxyvitamin D) and physical fitness levels. The aim of this study was to investigate the relationship between NAFLD risk and serum vitamin D levels combined with physical fitness in elderly adults using a non-invasive diagnostic approach. We enrolled 533 Korean ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 30, 2019 Category: Biochemistry Source Type: research

The implications of vitamin content in the plasma in reference to the parameters of carbohydrate metabolism and hormone and lipid profiles in PCOS
Publication date: Available online 26 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Małgorzata Szczuko, Viktoria Hawryłkowicz, Justyna Kikut, Arleta DrozdAbstractSo far, there have been no analyses of correlations between the level of water-soluble vitamins in women with polycystic ovary syndrome (PCOS) and hormone and lipid profiles as well as carbohydrate metabolism. The unpopular concept that PCOS may also be conditioned by a chronic infection leads to a suspicion that water-soluble vitamins may be involved in the struggle against PCOS. This is why the aim of this research wa...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 27, 2019 Category: Biochemistry Source Type: research

Structure-dependent retention of steroid hormones by common laboratory materials
Publication date: Available online 26 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Jeffrey M. McManus, Nima SharifiAbstractThe tendency of steroid molecules to adsorb to various materials, particularly plastics, has been known of for decades but has not received widespread attention in the scientific community, and a modern, systematic study is lacking. This adsorption is an important consideration for researchers working with steroid hormones as it could skew the results of various experiments. Here we show that steroids adsorb to various vessels used in experiments, including m...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 27, 2019 Category: Biochemistry Source Type: research

Ordered structure-forming properties of the intrinsically disordered AB region of hRXRγ and its ability to promote liquid-liquid phase separation
Publication date: Available online 24 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Katarzyna Sołtys, Andrzej OżyharAbstractThe retinoid X receptor (RXR) is a member of the nuclear receptor (NR) superfamily that occupies the central position among other NRs by forming both homodimers and heterodimers with other representatives of the family. RXR shares similar structural domains with other members of NRs. The major differences in the subtypes and isoforms of RXR are in the AB region. To date, there have been no data concerning the molecular properties of the AB region of hRXRγ ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 26, 2019 Category: Biochemistry Source Type: research

Targeting 1,25(OH)2D-Mediated Calcium Absorption Machinery in Proximal Colon with Calcitriol Glycosides and Glucuronides
Publication date: Available online 24 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): H. Jiang, R.L. Horst, N.J. Koszewski, J.P. Goff, S. Christakos, J.C. FleetAbstractHigh intestinal calcium (Ca) absorption efficiency is associated with high peak bone mass in adolescents and reduced bone loss in adulthood. Transepithelial intestinal Ca absorption is mediated by 1,25-dihydroxyvitamin D (1,25(OH)2D, calcitriol) through the vitamin D receptor (VDR). Most research on Ca absorption focuses on the proximal small intestine but evidence shows that large intestine plays a crucial role in wh...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 26, 2019 Category: Biochemistry Source Type: research