Simultaneous LC/MS/MS quantification of eight apolipoproteins in normal and hypercholesterolemic mouse plasma [Methods]
In conclusion, we present a robust, quantitative LC/MS/MS method for the multiplexed analysis of eight apolipoproteins in mice. This assay can be applied to investigate the effects of genetic manipulation or dietary interventions on apolipoprotein levels in plasma and isolated lipoprotein fractions. (Source: The Journal of Lipid Research)
Source: The Journal of Lipid Research - March 31, 2019 Category: Lipidology Authors: Wagner, R., Dittrich, J., Thiery, J., Ceglarek, U., Burkhardt, R. Tags: Methods Source Type: research
A new LC-MS assay for the quantitative analysis of vitamin K metabolites in human urine [Methods]
Vitamin K (VK), in both its phylloquinone and menaquinone forms, has been hypothesized to undergo - and β-oxidation on its hydrophobic side chain in order to generate the observed urinary metabolites, K acid I and K acid II, which are excreted primarily as glucuronide conjugates. Synthetic standards of K acid I, K acid II, and a putative intermediate metabolite, menaquinone (MK)1 -COOH, were used to develop and optimize a new atmospheric pressure negative chemical ionization LC-MS/MS assay for the quantitation of these compounds in urine from untreated individuals and subjects treated with a high dose VK supplement. V...
Source: The Journal of Lipid Research - March 31, 2019 Category: Lipidology Authors: McDonald, M. G., Yeung, C. K., Teitelbaum, A. M., Johnson, A. L., Fujii, S., Kagechika, H., Rettie, A. E. Tags: Methods Source Type: research
Ces1d deficiency protects against high-sucrose diet-induced hepatic triacylglycerol accumulation [Research Articles]
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. Triacylglycerol accumulation in the liver is a hallmark of NAFLD. Metabolic studies have confirmed that increased hepatic de novo lipogenesis (DNL) in humans contributes to fat accumulation in the liver and to NAFLD progression. Mice deficient in carboxylesterase (Ces)1d expression are protected from high-fat diet-induced hepatic steatosis. To investigate whether loss of Ces1d can also mitigate steatosis induced by over-activated DNL, WT and Ces1d-deficient mice were fed a lipogenic high-sucrose diet (HSD). We found that Ces1d-deficient mice...
Source: The Journal of Lipid Research - March 31, 2019 Category: Lipidology Authors: Lian, J., Watts, R., Quiroga, A. D., Beggs, M. R., Alexander, R. T., Lehner, R. Tags: Research Articles Source Type: research
An upstream enhancer regulates Gpihbp1 expression in a tissue-specific manner [Research Articles]
Glycosylphosphatidylinositol-anchored high density lipoprotein–binding protein 1 (GPIHBP1), the protein that shuttles LPL to the capillary lumen, is essential for plasma triglyceride metabolism. When GPIHBP1 is absent, LPL remains stranded within the interstitial spaces and plasma triglyceride hydrolysis is impaired, resulting in severe hypertriglyceridemia. While the functions of GPIHBP1 in intravascular lipolysis are reasonably well understood, no one has yet identified DNA sequences regulating GPIHBP1 expression. In the current studies, we identified an enhancer element located ~3.6 kb upstream from exon 1 of mous...
Source: The Journal of Lipid Research - March 31, 2019 Category: Lipidology Authors: Allan, C. M., Heizer, P. J., Tu, Y., Sandoval, N. P., Jung, R. S., Morales, J. E., Sajti, E., Troutman, T. D., Saunders, T. L., Cusanovich, D. A., Beigneux, A. P., Romanoski, C. E., Fong, L. G., Young, S. G. Tags: Research Articles Source Type: research
Rosiglitazone remodels the lipid droplet and britens human visceral and subcutaneous adipocytes ex vivo [Research Articles]
Treatment with PPAR agonists in vivo improves human adipocyte metabolism, but the cellular mechanisms and possible depot differences in responsiveness to their effects are poorly understood. To examine the ex vivo metabolic effects of rosiglitazone (Rosi), we cultured explants of human visceral (omental) and abdominal subcutaneous adipose tissues for 7 days. Rosi increased mRNA levels of transcriptional regulators of brite/beige adipocytes (PGC1α, PRDM16), triglyceride synthesis (GPAT3, DGAT1), and lipolysis (ATGL) similarly in adipose tissues from both depots. In parallel, Rosi increased key modulators of FA oxidati...
Source: The Journal of Lipid Research - March 31, 2019 Category: Lipidology Authors: Lee, M.-J., Jash, S., Jones, J. E. C., Puri, V., Fried, S. K. Tags: Research Articles Source Type: research
