Revista Brasileira de Farmacognosia 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Determination of phenolic profile by HPLC–ESI-MS/MS, antioxidant activity, in vitro cytotoxicity and anti-herpetic activity of propolis from the Brazilian native bee Melipona quadrifasciata
This study determined the chromatographic and mass spectrometry profile along with the biological activity of propolis produced by the native Brazilian bee Melipona quadrifasciata. A hydroalcoholic crude extract was prepared and partitioned in solvents of different polarities, generating aqueous, dichloromethane, ethyl acetate, butanol and insoluble fractions. The phenolic and flavonoid content was also determined in crude aqueous and methanolic extracts as well. The antioxidant activity was determined by three different methods and correlated with the phenolic and flavonoid content. Extracts and fractions were tested agai...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Development and validation of a high-performance thin layer chromatography method for the simultaneous quantitation of α- and γ-mangostins in Thai stingless bee propolis
ABSTRACT Stingless bees (Apoidea) are widely distributed and commercially cultivated in artificial hives in fruit gardens. Their propolis are commonly used in traditional medicine to treat various diseases (e.g., abscesses, inflammations, and toothaches) and as a constituent of numerous health products. Thus, this study aimed to (i) develop and validate a high-performance thin layer chromatography method for the quantitation of major active constituents ( α - and γ -mangostins) in propolis produced by five stingless bee species (Tetragonula fuscobalteata Cameron, T. laeviceps Smith, T. pagdeni Schwarz, Lepidotrigona term...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro
ABSTRACT To evaluate the anti-Helicobacter pylori activity of the major polyphenol compounds of propolis and their cellular damage, both as single molecule or in combination. Honey bees propolis were fractionated by using CPC and preparative HPLC. Four major polyphenols (chrysin, pinocembrin, galangin and caffeic acid phenethyl ester) were identified by thin layer chromatography – mass spectroscopy and liquid chromatography – mass spectroscopy. These compounds inhibited both ATCC and clinical H. pylori strains, with caffeic acid phenethyl ester being the most active. The four compounds presented minimum inhibitory conc...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Partial characterization of ethanolic extract of Melipona beecheii propolis and in vitro evaluation of its antifungal activity
ABSTRACT The ethanolic extract of Melipona beecheii Bennett 1831, propolis from Yucat án Mexico was evaluated in vitro for the determination of its phenolic compound content, antioxidant capacity and antifungal activity. The results were compared against those of the ethanolic extract of Apis mellifera propolis. The total phenolic content, flavonoid content and flavanones – dihydroflavonols content were assessed by colorimetric methods. The antifungal activity was assessed in vitro against Candida albicans. For the ethanolic extract of M. beecheii propolis; total phenolic content, was 263.25 ± 8.78 µg/ml, total flavon...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Chemical characterization, antioxidant and anti-HIV activities of a Brazilian propolis from Ceará state
ABSTRACT Propolis (bee glue) a product of Apis mellifera L. is a resinous mixture containing chiefly beeswax and resin harvested by bees from plant leaves, buds and exudates. Extracts of a propolis sample from Salitre, a municipality of Cear á state (northeast Brazil) were obtained with solvents of increasing polarity (hexane, chloroform, ethyl acetate and methanol). A chemical profile was carried out by GC – EI-MS and HPLC – DAD – ESI-MS/MS. Lupenone, lupeol, octanoic acid tetracosyl ester and octanoic acid hexacosyl ester were identified by GC – EI-MS. Antioxidant activity was evaluated by the DPPH and β -carot...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
The role of seasonality on the chemical composition, antioxidant activity and cytotoxicity of Polish propolis in human erythrocytes
ABSTRACT Propolis extracts possess beneficial biological effects, such as antioxidant activity. However, the composition of propolis and biological properties of its extracts depend on many factor, including time of harvesting. The main purpose of this study was to evaluate the seasonal effect on the phenolic profile of Polish propolis extracts and their antioxidant activity. Propolis samples were collected from the same apiary during three seasons of the year. The chemical composition (contents of phenolic acids and flavonoids) of ethanolic propolis extracts was determined by ultra-performance liquid chromatography equipp...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Chemical composition of propolis extract and its effects on epirubicin-induced hepatotoxicity in rats
ABSTRACT Propolis is a natural substance, produced by honeybees from the resin of various plants. The purpose of this study was to determine the chemical composition and evaluate the hepatoprotective effect of ethyl acetate extract of propolis from Tigzirt, against the toxicity induced by epirubicin which is a anticancer agent, and belongs to the family of antracyclines. The study included thirty male Wistar albino rats divided into five groups. The rats received the extraction of propolis or the quercetin orally for 15 days. The hepatotoxicity was promoted by injection epirubicin (i.v.) with a cumulative dose of 9 mg/kg. ...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Antileishmanial activity and chemical composition from Brazilian geopropolis produced by stingless bee Melipona fasciculata
This study evaluated the antileishmanial activity in vitro, cytotoxicity and chemical composition of geopropolis produced by M. fasciculata in the savannah region of Maranh ão, Brazil. The geopropolis extract was obtained through maceration using in 70% ethanol. The hydroalcoholic extract of geopropolis after liquid – liquid partition yielded the hexane, chloroformic, ethyl acetate and hydroalcoholic fractions. Antileishmanial activity was evaluated against promastigote and intracellular amastigote of Leishmania amazonensis. Cytotoxic was realized in BALB/c mice peritoneal macrophages. Chemical analysis was performed by...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Geopropolis gel for the adjuvant treatment of candidiasis – formulation and in vitro release assay
This study analyzed the antifungal activity of the geopropolis extract from Candida spp., developed a gel formulation with this extract and analyzed the delivery of bioactives (kinetics release) in the formulation and their chemical profile by UH PLC-PDA-qTOF-MS/MS. Three different gels were prepared using the geopropolis extract, carbomer, propylene glycol, and water. Formulations with different amounts of propylene glycol were investigated. Physical, visual, pH, viscosity, adhesion, spreadability, leakage, and in vitro release tests were p erformed in the proposed formulations. Antifungal tests with the geopropolis ethan...
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
Effect of Bulgarian propolis on the oral microflora in adolescents with plaque-induced gingivitis
In conclusion, the addition of Bulgarian propolis to the toothpaste improved the gingival health in adolescents with moderate plaque-induced gingivitis and resulted in increas ed activity against potential periodontal and cariogenic pathogens. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - August 27, 2019 Category: Drugs & Pharmacology Source Type: research
An eco-friendly method for extraction and quantification of flavonoids in Dysphania ambrosioides
ABSTRACT Dysphania ambrosioides (L.) Mosyakin and Clemants (Syn: Chenopodium ambrosioides L.), Amaranthaceae, is a plant with antibacterial, antifungal, antioxidant, antiparasitic and antitumor properties that is commonly used in Brazilian folk medicine. In this work we performed the optimization of ultrasound-assisted extraction of flavonoids in the aerial parts of D. ambrosioides. The flavonoid concentrations, as rutin equivalents, were quantified with the aid of a validated spectrophotometric method. The Box – Behnken (33) design with response surface methodology, for the independent variables, extraction time, temper...
Source: Revista Brasileira de Farmacognosia - May 27, 2019 Category: Drugs & Pharmacology Source Type: research
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum
This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, wi...
Source: Revista Brasileira de Farmacognosia - May 27, 2019 Category: Drugs & Pharmacology Source Type: research
An optimization approach of dynamic maceration of Centella asiatica to obtain the highest content of four centelloids by response surface methodology
ABSTRACT Centella asiatica (L.) Urb., Apiaceae, is commonly used as food, food supplement, and medicine. Development of the extraction process to obtain the high extent of the active compound is necessary. So, the response surface methodology was used in this work to optimize the dynamic maceration of C. asiatica to obtain the highest content of the four centelloids including asiatic acid, madecassic acid, asiaticoside, and madecassoside. Two factors: extraction temperature and extraction time, were studied. The content of four centelloids was observed. After the extraction of C. asiatica using ethanol, the content of four...
Source: Revista Brasileira de Farmacognosia - May 27, 2019 Category: Drugs & Pharmacology Source Type: research
Chronic hypoglycemic effect and phytochemical composition of Smilax moranensis roots
In conclusion, this study confirms that the roots of S. moranensis have hypoglycemic properties and suggests that the isolated compounds are potentially involved in this effect. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - May 27, 2019 Category: Drugs & Pharmacology Source Type: research
In vivo anti-hyperuricemic activity of sesquiterpene lactones from Lychnophora species
ABSTRACT Hyperuricemia is the main cause of gout, an inflammation induced by uric acid deposition in joints. Drugs available present side effects, so there is a need for new treatment alternatives. Lychnophora species are used in folk medicine to treat inflammation, rheumatism and muscle pain. Goyazensolide (10 mg/kg), eremantholide C (25 mg/kg) and lychnopholide (25 mg/kg), sesquiterpene lactones isolated from Lychnophora species were previously studied and showed anti-hyperuricemic effects in mice. However, the mechanisms of this effect were not elucidated. The methodology of this study consisted in treatment of hyperuri...
Source: Revista Brasileira de Farmacognosia - May 27, 2019 Category: Drugs & Pharmacology Source Type: research
