Thermal and chemical characterization of Dicksonia sellowiana extract by means of thermal analysis
Abstract Dicksonia sellowiana Hook., Dicksoniaceae, is a tree-fern which is being recently used in medicine mainly for its phytotherapic activities. While several other studies have focused on D. sellowiana extract characterization in terms of its biological and antioxidant activity, the novelty of this work aims to understand the fate of this extract during thermal disposal through thermogravimetry/differential thermal analysis, thermogravimetry/mass spectrometry, Fourier transform infrared spectroscopy and elemental analysis, to further characterize this plant's extract. Thermal analysis revealed mass loss within three w...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Nanosuspension of quercetin: preparation, characterization and effects against Aedes aegypti larvae
Abstract Aedes aegypti (Diptera: Culicidae) is the main vector of some neglected diseases, including dengue. It is very important to develop formulations that increase effectiveness of vector control with low toxicity. Quercetin is a plant-derived flavonoid that modulates the development of some insects. The low water solubility of quercetin impairs the development of water-dispersible commercial products. To circumvent this problem, the preparation of nanoformulations is considered promising. Thus, this study aimed to evaluate the effect of bulk and quercetin nanosuspension against A. aegypti larvae and also to investigat...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Optimization of flavonoid extraction from Passiflora quadrangularis leaves with sedative activity and evaluation of its stability under stress conditions
Abstract Passiflora species have been widely used in folk medicine as tranquilizers, and previous pharmacological studies have reported sedative activity for P. quadrangularis L., Passifloraceae, leaf extracts. The aim of this work was to contribute to the standardization of P. quadrangularis leaf extract with sedative activity. For this purpose, the extraction of total flavonoids was optimized, evaluating variables such as drug-solvent ratio, extraction solvents and extraction time, using Response Surface Methodology. The stability of total and individual flavonoids on the optimized extract of P. quadrangularis leaves und...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Antitumor activity and toxicity of volatile oil from the leaves of Annona leptopetala
Abstract Annona leptopetala (R.E.Fr.) H. Rainer, Annonaceae, is used in folk medicine like antitumor and anti-inflammatory. The aim of this study was to determine chemical composition, toxicity and antitumor activity of A. leptopetala leaves volatile oil. Fresh leaves were hydrodistilled and then the volatile oil chemical composition was assessed by gas chromatography and mass spectrometry. Toxicity was assessed using haemolysis, micronucleus and acute toxicity protocols. Antitumor effects were determined in vitro and in vivo, using sulforhodamine B assay and sarcoma 180 murine tumor model, respectively. Spathulenol was th...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Phenolic-antioxidant capacity of mango seed kernels: therapeutic effect against viper venoms
In this study, mango seed kernels extract contained a considerable amount of phenolics and flavonoids (17,400 and 3325 mg/100 g seed, respectively). The HPLC profiling revealed that hesperidin was the major phenolic compound of the mango seed kernels extract. This is the first report find hesperidin in mango extracts. The phenolic compounds of mango seed kernels extract were effective in scavenging free radicals of DPPH and ABTS with IC50 values of 47.3 and 7.9 µg/ml, respectively. The total antioxidant activity of mango seed kernels extract based on the reduction of molybdenum was also measured. The phenolic compoun...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Hydroethanolic extract of Tropaeolum majus promotes anxiolytic effects on rats
This study investigated the central pharmacological actions of the prolonged treatment with a hydroethanolic extract of T. majus in male Wistar rats in the elevated plus maze and hole-board behavioral models. For this, rats were daily treated with distillated water (negative control); diazepam (1 mg/kg) or hydroethanolic extract of T. majus (75, 150 and 300 mg/kg), for 29 days (by gavage) and were submitted to elevated plus maze and hole-board. Animals treated with all hydroethanolic extract of T. majus or diazepam doses increased the percentage of entries in open arms when compared to control group. However, only treatmen...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

LC–MS/MS determination of deoxyelephantopin, a novel anti-tumor candidate in rat plasma and its application to a pharmacokinetic study in rats
Abstract A specific, sensitive and robust LC – MS/MS method was developed and validated for the quantification of deoxyelephantopin in rat plasma using simvastatin as an internal standard as per regulatory guidelines of Bioanalytical Method Validation. Plasma sample was prepared through liquid-liquid extraction. Chromatographic separation was performed on an Agela-C18 analytical column (1.8 µm, 2.1 mm × 50 mm) with an isocratic mobile phase consisting of 0.05% formic acid (dissolved in acetonitrile) and water (55:45, v/v) at a flow rate of 0.5 ml/min. The column oven was maintained at 40 ºC and the i...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacological and chemical properties of some marine echinoderms
Abstract Echinoderms have attracted the attention of scientists over the past few years after identifying a variety of unique structures endowed by interesting biological properties. However, the Moroccan coast biodiversity is still uninvestigated. In our ongoing attempts to valorize the rich Moroccan marine environment, this study aimed at assessing the antimicrobial activity of extracts obtained from three echinoderms Astropecten irregularis, Luidia sarsi and Ophiura albida against the human pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica and Bacillus subtilis. Moreover, th...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Acetylcholinesterase inhibitory activity of a neurosteroidal alkaloid from the upside-down jellyfish Cassiopea andromeda venom
In conclusion, the isolated neurosteroidal alkaloid from Cassiopea andromeda was a potent antiAChE agent with strong binding to both the catalytic and peripheral sites of acetylcholinesterase that correlated well with the experimental data. Further s tudies are required to determine whether androtoxin B could be a potential treatment for Alzheimer's disease. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Salvastearolide, a new acyl-glyceride, and other constituents from the seeds of Salvadora persica
Abstract The chemical investigation of the n-hexane fraction of Salvadora persica L., Salvadoraceae, seeds afforded a new stearic acid ester, salvastearolide, together with five other phytosteroids identified as stigmasterol, β -sitosterol, Δ 7-campesterol, Δ 7-avenasterol and campesterol. Their structures were established on the basis of extensive spectroscopic methods including 1D and 2D NMR experiments and HRESI mass spectrometry. In addition, salvastearolide and the isolated fractions were tested for their cytotoxicity against human cancer cell lines MCF-7, MDA-MB-231 and HT-29. The n-hexane fraction e...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Carnosol from Lepechinia mutica and tiliroside from Vallea stipularis: Two promising inhibitors of BuChE
Abstract Lepechinia mutica (Benth.) Epling, Lamiaceae, and Vallea stipularis L.f., Elaeocarpaceae, are the object of the present study. These plants are endemic to the Andean region and have attracted our attention on the basis of interesting results obtained in a preliminary anticholinesterase screening. Actually, carnosol and tiliroside, isolated from L. mutica and V. stipularis, respectively, have shown a promising selective inhibitory activity against butyrylcholinesterase. Specifically, the anti-butyrylcholinesterase activity of carnosol was 5.15 µM and that of tiliroside was 52.9 µM, compared to 8.568 &pl...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro antileishmanial and antitrypanosomal activity of compounds isolated from the roots of Zanthoxylum tingoassuiba
Abstract Five coumarins (5,7,8-trimethoxycoumarin (1), sabandin (2), cubreuva lactone (3), 5,7-dimethoxycoumarin (5) and braylin (6)), seven furoquinoline alkaloids (isopimpinelin (4), pteleine (7), maculine (8), skimianine (10), robustine (11), y-fagarine (12) and dictamine (13) and the furofuran type lignin syringaresinol (9)) have been identified for the first time in the roots of Zanthoxylum tingoassuiba A. St.-Hil., Rutaceae. Pure compounds 1, 6, 9, 12 were tested against Leishmania amazonensis parasites and epimastigotes forms of Trypanosoma cruzi. All the tested products displayed an antiparasitic activity similar t...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Trifolium pratense: Friable calli, cell culture protocol and isoflavones content in wild plants, in vitro and cell cultures analyzed by UPLC
Abstract Trifolium pratense L., Fabaceae, is a rich source of isoflavones and has become the focus of several studies related to its phytoestrogenic activity. The aim of this study was to establish germination and cell cultures protocol for T. pratense and quantify isoflavones content in cell cultures, in vitro cultured and wild plants harvested in two different seasons. Murashige Skoog medium supplemented with naphthalene acetic acid and kinetin was able to produce the highest formation of friable calli. Calli cultures were analyzed qualitatively after 60 days of culture, and in vitro plants after 30, 45 and 60 days of cu...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Comprehensive chemical analysis of the flower buds of five Lonicera species by ATR-FTIR, HPLC-DAD, and chemometric methods
Abstract Lonicera japonica Thunb., Caprifoliaceae, has been employed in traditional Chinese medicine for thousands of years. However, it is frequently confused with closely related species, and thus, a mixture of these species is commonly used. The sources of Lonicera species must be accurately and rapidly determined to ensure the clinical efficacy of herbal medicines. Attenuated total reflectance Fourier transform infrared spectroscopy and high-performance liquid chromatography with a diode array detector, combined with chemometrics methods, were used to comprehensively evaluate the Lonicera quality. The infrared fingerpr...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

HPLC fingerprint and simultaneous quantitative analysis of phyllanthin and hypophyllanthin for identification and authentication of Phyllanthus niruri from related species
Abstract A precise and accurate method for the identification and authentication of Phyllanthus niruri L. from P. debilis Klein ex Willd. and P. urinaria L., Phyllanthaceae, was developed using high-performance liquid chromatography. Chromatographic fingerprint analysis was combined with simultaneous quantification of phyllanthin and hypophyllanthin for the developed method. Phyllanthin and hypophyllanthin were successfully separated and quantified under this proposed method. The highest amount of phyllanthin and hypophyllanthin was found in P. niruri compared to P. debilis and P. urinaria. Fingerprint chromatogram of the ...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacognostical studies of Premna microphylla
Abstract Various traditional systems of medicine enlightened the importance of Premna microphylla Turcz., Lamiaceae, medicinally. The present study was carried out to provide a scientific basis of the identification and the authenticity of P. microphylla with the help of pharmacognostical parameters, which is not done before. Roots, stems, and leaves of P. microphylla were collected for pharmacognostical studies involving macros, microscopic evaluation, physicochemical parameters analysis like fluorescence analysis and thin layer chromatography, in addition with DNA barcodes of internal transcribed spacer and psbA-trnH reg...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Morpho-anatomical characters of Limonium brasiliense leaves
Abstract Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, is a coastal herb of the Southern Brazilian states and Rio de Janeiro State. In folk medicine, it is used in the treatment of female genitourinary tract infections and menstrual cycle irregularities. The aim of this study was to describe morpho-anatomical characters with details on venation pattern of L. brasiliense leaves, collected on the coast of Rio Grande do Sul State, in Southern Brazil. Leaf samples fixed in FAA50 (5% formaldehyde, 5% acetic acid and 90% ethanol: water mixture 50:50 v/v) and 1% glutaraldehyde and preserved in 70% ethanol, were histologic...
Source: Revista Brasileira de Farmacognosia - October 11, 2018 Category: Drugs & Pharmacology Source Type: research

Traditional phytomedicines for gynecological problems used by tribal communities of Mohmand Agency near the Pak-Afghan border area
In conclusion, the documented flora that is particularly important to medicinal plants may be researched in the future to discover new pharmaceutical, neutraceutical and other pharmacological agents against gynecological complaints. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro release and anti-herpetic activity of Cymbopogon citratus volatile oil-loaded nanogel
This study aimed to prepare hydrogel containing Cymbopogon citratus (DC.) Stapf, Poaceae, volatile oil encapsulated in poly (d,l-lactide-co-glycolide) nanoparticles and to evaluate its in vitro anti-herpetic activity. Polymeric nanoparticles were prepared by solvent emulsification-diffusion method and incorporated in carbomer hydrogels. In vitro release profiles for the nanogel, loaded nanoparticles and hydrogel containing free oil were evaluated by dialysis. Inhibitory activities against Herpes simplex for the formulations were investigated in Vero cells. Hydrogel was developed using nanoparticles with mean diameter of 21...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Evaluation of hepatoprotective activity of Syringa oblata leaves ethanol extract with the indicator of glutathione S-transferase A1
Abstract The leaves of Syringa oblata Lindl., Oleaceae, had been extensively used as a folk medicine to treat various infections, heal inflammations, icteric hepatitis and acute mastitis. The study was designed to evaluate the hepatoprotective activity of S. oblata leaves ethanol extract against CCl4-induced hepatotoxicity in primary hepatocytes and mice with the indicator of glutathione S-transferase alpha 1. The hepatoprotective effects of S. oblata leaves ethanol extract were evaluated on the basis of liver histopathology and biochemical parameters as well as hepatic oxidative stress markers. The results showed that CCl...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Different processing methods change the oral toxicity induced by Sophora alopecuroides seeds and the contents of five main toxic alkaloids from the ethanol extracts determined by a validated UHPLC–MS/MS assay
This study investigated the influence of different processing methods on the oral toxicity of Sophora alopecuroides L., Fabaceae, seeds in mice and on the contents of five known toxic-effective quinolizidine alkaloids from the ethanol extracts quantified by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry. It provides an evidence to elucidate the possible reasons why vinegar-processing and parching methods significantly decrease the acute oral toxicity induced by S. alopecuroides and why wine-processing method increases it instead (demonstrated by measurement of LD50 and histopathologi...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

1H NMR-based nontargeted metabonomics study of plasma and urinary biochemical changes in Kudouzi treated rats
Abstract Kudouzi (Sophora alopecuroides L., Fabaceae) is an effective folk medicine, but it always causes a hepatic and renal toxicity in clinical therapy. The toxic mechanism remains unclear. This paper detected the urinary and plasma metabolites alteration by 1H NMR-based metabonomics study in Kudouzi-induced rats to evaluate the toxic mechanism for clinical security. The male Sprague-Dawley rats were orally dosed with 0.5 and 1 g Kudouzi/kg weight once per day for consecutive 14 days. Urine samples were collected at day − 1 (before treatment), and days 7, 14, and 21 for NMR analysis, respectively. Plasma samples w...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

(2R,3S)-Pinobanksin-3-cinnamate promotes osteoblast differentiation through cAMP and cGMP pathways
In this study, we investigated the effects of a new flavone (2R,3S)-pinobanksin-3-cinnamate, isolated from the metabolites of the endophytic fungus Penicillium sp. FJ-1 of Acanthus ilicifolius L., Acanthaceae, on osteoblast differentiation by using MC3T3-E1 cells. It was observed that (2R,3S)-pinobanksin-3-cinnamate promoted osteoblast differentiation, as evidenced by increased mineralization process and alkaline phosphatase activity, as well as expression of genes encoding the bone differentiation. Moreover (2R,3S)-pinobanksin-3-cinnamate treatment upregulated the gene expression of wingless-type MMTV integration site fam...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
Abstract Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew& R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 µM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells i...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
Abstract A selective and sensitive liquid chromatography tandem with mass spectrometry was developed and validated for accurate determination of monotropein in rat plasma and tissues. All biological samples were prepared by simple protein precipitation method using catalpol as an internal standard. The analyte and internal standard were separated on a C18 analytical column with 2 min of run time, at flow rate of 0.5 ml/min. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped with negative-ion electrospray ionization by selected-reaction monitoring of the transitions at m/z 389 → 147 fo...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Bioactive components, antioxidant, and anti-inflammatory activities of the wood of Albizia myriophylla
Abstract The scientific basis corresponding with the folkloric use of Albizia myriophylla Benth., Fabaceae, for the treatment of inflammation-related diseases was established by measuring antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl, 2,2 ′ -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) free radicals, and ferric reducing antioxidant power assays as well as anti-inflammatory effect using nitrite assay and ethyl phenylpropiolate (EPP)-induced rat ear edema model. Both ethanol extract (DPPH, IC50 46.23 µg/ml; ABTS, IC50 57.14 µg/ml; FRAP, 950.14 mM Fe (II)/g) and dichloromethane fraction (DPPH...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Physicochemical, biological and release studies of chitosan membranes incorporated with Euphorbia umbellata fraction
Abstract Formulations containing chitosan incorporated with methanolic fraction of Euphorbia umbellata (Pax) Bruyns, Euphorbiaceae, were studied aiming future applications of this new material as medicine. In order to investigate potential interactions between chitosan and the methanolic fraction (10, 50 and 100% in relation to the amount of chitosan) physicochemical characterization was performed by scanning electron microscopy, density, differential scanning calorimetry, thermogravimetry, X-ray diffraction, Fourier-transform infrared spectroscopy and colorimetry techniques. The phenolic compounds released from the chitos...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

The role of tannins as antiulcer agents: a fluorescence-imaging based study
Abstract Condensed tannins have been used for many years in folk medicine to treat gastric problems. The mechanism of action that explains why tannins improve gastritis symptoms is based on their ability to chelate metals, antioxidant activity, and their complexation power with other molecules. Even though these uses are well-known, the requirements to become an herbal medicine are much more complex. Herein, we analyzed Stryphnodendron rotundifolium Mart., Fabaceae, extract using MALDI for tannin characterization and carried out a fluorescence-imaging study to prove the gastroprotective effects of tannins as coating agents...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

A new feruloyl glyceride from the roots of Asian rice (Oryza sativa)
This study represents the first report of feruloyl glycerides (1 – 2) identified in O. sativa. In addition, the identification of compound 3 is reported from Asian rice (O. sativa) for the first time. The cytotoxic activities of the isolates 1 – 3 were evaluated by determining their inhibitory effects on A2780 human ovarian carcinoma cells. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Evaluation of the chemical composition and variability of the volatile oils from Trembleya parviflora leaves
This study provides data that can be applied for the quality control of powdered leaves and is the first description of the chemical composition and variability of the volatile oils from the leaves of T. parviflora. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Multivariate statistical analysis of morpho-anatomical data of nine sect. Caulopterae species (Baccharis – Asteraceae) used in folk medicine
Abstract Baccharis species belonging to sect. Caulopterae are difficult to identify. Most countries are controlling the quality of herbal medicines destined for the internal market or export. "Carquejas" are used arbitrarily for the same medicinal purposes and only three species of sect. Caulopterae are official herbal medicines. In the present study, a morpho-anatomical and statistical analysis was performed with nine species of sect. Caulopterae: Baccharis articulata, B. crispa, B. gaudichaudiana, B. microcephala, B. penningtonii, B. phyteumoides, B. sagittalis, B. triangularis and B. trimera, emphasizing the i...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Morphoanatomical and histochemical characterization of Larrea species from Northwestern of Argentina
Abstract Larrea divaricata Cav., L. cuneifolia Cav. and L. nitida Cav., Zygophyllaceae, are evergreen xerophytic shrubs occurring in Northwestern Argentina used in traditional medicine. The aim of this work was to characterize the morphology, anatomy and histochemistry of the vegetative organs of three Larrea species by light and scanning electron microscopy in order to provide supporting data for their correct identification and to determine the site of synthesis and accumulation of its main active compounds. The shape, number and percentage of coalescence of leaflets, presence or absence of mucrones and rachis and the sh...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Leaf morphoanatomy of "mororó" (Bauhinia and Schnella, Fabaceae)
Abstract Bauhinia L. and Schnella (Raddi.) Wund. are popularly known in Brazil as "moror ó". The leaves and stem bark are used in folk medicine for various purposes, especially against diabetes. Morphoanatomical studies of the leaves of Bauhinia cheilantha (Bong.) Steud., B. pentandra (Bong.) Steud., B. ungulata L. and Schnella outimouta (Aublet) Wund., tribe Cercidae, subtribe Bauhini inae (Benth.) Walp., were carried out as subsidies to the quality control of their etnodrugs and their derivatives, as well as an additional support to their taxonomy. The morphological and anatomical studies employed tradit...
Source: Revista Brasileira de Farmacognosia - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Evaluation of anxiolytic-like effect of 7-epiclusianone isolated from Garcinia brasiliensis in mice
ABSTRACT Garcinia brasiliensis Mart., Clusiaceae, species became the target of studies for some years because it has several compounds including polyprenylated benzophenones, as 7-epiclusianone. This benzophenone has several properties, such as leishmanicidal, anti-inflammatory and antinociceptive effects, however still did not be studied anxiolytic activity. For this, the open field and elevated plus maze tests were used in order to evaluate the effect of administration of 7-epiclusianone (isolated from G. brasiliensis) on behavioral performance. Swiss male mice (n = 10 per group) were pre-treated with vegetable oil (10 m...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Anti-angiogenic activity of iridoids from Galium tunetanum
ABSTRACT The phytochemical study of Galium tunetanum Lam., Rubiaceae, leaves led to the isolation of 13 compounds from the chloroform – methanol and the methanol extracts, including six iridoid glycosides, one non-glycoside iridoid, two p-coumaroyl iridoid glycosides, two phenolic acids, and two flavonoid glycosides. The structural determination of the isolated compounds was performed by mono- and bidimensional NMR spectroscopic data, as well as ESI-MS experiments. All compounds were isolated from this species for the first time. The anti-angiogenic effects of the isolated iridoids were also reported on new blood ves...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Fingerprint and multi-ingredient quantitative analyses for quality evaluation of hawthorn leaves and Guang hawthorn leaves by UPLC–MS
ABSTRACT This paper aimed to evaluate the quality of hawthorn leaves and Guang hawthorn leaves by an UPLC – MS method from two aspects, fingerprint analysis and multi-ingredient quantification. Chromatographic separation was carried out on an UPLC system, the standardized characteristic fingerprints was established by Similarity Evaluation System for chromatographic fingerprinting of traditional Chinese medicine and cluster analysis. Eight components were simultaneously determined by mass spectrometry in multiple reaction-monitoring mode. The method was validated in terms of linearity (R2> 0.9971), intraday and in...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Phytochemical analysis of Juglans regia oil and kernel exploring their antinociceptive and anti-inflammatory potentials utilizing combined bio-guided GC–FID, GC–MS and HPLC analyses
ABSTRACT Juglans regia L., Juglandaceae, is broadly used due to its immunomodulatory effects, potentials in protecting against many sever-disorders, and high safety-profile. The aim of this work is to make a phytochemical analysis of J. regia oil and kernel exploring their antinociceptive and anti-inflammatory potentials utilizing combined bio-guided gas chromatography with mass spectrometer (GC – MS), gas chromatography with flame ionization detection (GC – FID) and reversed-phase high-performance liquid chromatography (RP – HPLC) analyses. Combined bio-guided GC – MS, GC – FID and RP –...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Nests of “caba-leão” wasps (Sceliphron sp., Sphecidae) used in traditional medicine by riverine communities of the Jaú and Unini Rivers, Amazon, Brazil: ethnopharmacological, chemical and mineralogical aspects
ABSTRACT This research contributes to the study of substances present in homemade remedies used by the riverine communities of the Amazon, which are the result of insect – mineral – vegetable oil-interaction. The results of the analyses show the main components (inorganic and organic components) of the “ caba-le ão ” wasps nests (Sceliphron sp., Sphecidae) used by “ caboclos ” as a topical medication to treat mumps and earaches. The ethnopharmacological data collection consisted of samples of wasps nests and soil, as a source of inorganic elements, from the Ja ú and Unini R...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

β-Hydroxyisovalerylshikonin promotes reactive oxygen species production in HCT116 colon cancer cells, leading to caspase-mediated apoptosis
ABSTRACT Although β -hydroxyisovalerylshikonin is suggested as a potential therapeutic agent for preventing various cancers, the underlying molecular mechanisms are not completely understood. In the present study, we investigated whether β -hydroxyisovalerylshikonin enhances apoptosis by triggering reactive oxygen species production in colon cancer HCT116 cells. β -Hydroxyisovalerylshikonin significantly inhibited the viability of HCT116 cells with maximum inhibition at 4 µM. Furthermore, treatment with β -hydroxyisovalerylshikonin subsequently increased sub-G1 cells and annexin-V+ cell population...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Bioactive dicaffeoylquinic acid derivatives from the root extract of Calea urticifolia
ABSTRACT Calea urticifolia (Mill.) DC., Asteraceae, is a native plant of the Yucatan Peninsula used in traditional medicine to treat inflammation and pain. The bioassay-guided purification of the ethanol root extract allowed the isolation of the main bioactive metabolites, which were identified as an inseparable mixture of thymol (1) and 3-methyl-4-isopropylphenol (2), together with 3,4-O-dicaffeoylquinic acid methyl ester (3), 3,4-O-dicaffeoyl-epi-quinic acid methyl ester (4), 3,5-O-dicaffeoyl-epi-quinic acid methyl ester (5) and 3,5-O-dicaffeoylquinic acid (6). The results showed that the analgesic activity detected in t...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines
ABSTRACT The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1",2"-epoxy-3"-hydroxy-3"-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2',6'-dimethoxy-4',5'-(2",2"-dimethyl)-pyranochalcone, pongachalcone, maackiain, β -sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving ...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Antioxidant activity evaluation of dried herbal extracts: an electroanalytical approach
ABSTRACT The prevention of chronic and degenerative diseases, is a health concern deeply associated with oxidative stress. Such progressive phenomena can be avoided through exogenous antioxidant intake, which set up a reductant cascade, mopping up damaging free radicals. Medicinal herbs are commonly associated with high antioxidant potential, and hence their health benefits. The commerce of dried herbal extracts movements a big portion of developing countries economy. The determination of medicinal herbs the antioxidant activity capacity is of utmost importance. The assessment of antioxidant activity in phytotherapics is m...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Lipoxygenase inhibitors flavonoids from Cyperus rotundus aerial parts
ABSTRACT Cyperus rotundus L. (Suada, Sueda, family: Cyperaceae) is vastly spread in several world's subtropical and tropical regions. It had variable traditional uses and bioactivities. A new flavonol derivative: cyperaflavoside (myricetin 3,3',5'-trimethyl ether 7-O- β -D-glucopyranoside) and five flavonoids: vitexin, orientin, cinaroside, quercetin 3-O- β -D-glucopyranoside, and myrcetin 3-O- β -D-glucopyranoside were separated from the methanolic extract of C. rotundus aerial parts. Their structures were verified based on UV, IR, NMR (1D and 2D), HRESIMS, and comparison with literature. All metabolites we...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Optimization for extraction of an oil recipe consisting of white pepper, long pepper, cinnamon, saffron, and myrrh by supercritical carbon dioxide and the protective effects against oxygen–glucose deprivation in PC12 cells
This study is to investigate the most efficient extractives of extracting oil recipe for stroke treatment and the protective effects on an oxygen and glucose deprivation model in PC12 cells. An orthogonal experimental design L9 (34) was carried out for oil recipe's optimization with supercritical CO2 fluid extraction. 2-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and enzyme-linked immunosorbent assay were conducted to evaluate cell activity and indexes in the cell lysate. The result showed that the optimum extraction condition was 30 Mpa, 50 ºC, 100 min, the extracts were analyzed by gas chromatography ...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Phytochemistry and antimicrobial activity of Campomanesia adamantium
ABSTRACT Campomanesia adamantium (Cambess.) O. Berg., Myrtaceae, is a plant popularly used for its anti-inflammatory, anti-diarrhoeal and urinary antiseptic activities. The aims of this study were to obtain the crude ethanolic extract and the hexane, dichloromethane, ethyl acetate, aqueous and concentrated aqueous tannin fractions from C. adamantium leaves, perform biomonitored fractionation to isolate and identify chemical compounds, study the chemical composition of the volatile oils of the leaves and flowers and test the antimicrobial activity of the ethanolic extract, fractions, isolated substances and volatile oils. P...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Phytochemical study of Harrisia adscendens
The objective of this study was to perform the isolation and identification of the secondary metabolites obtained from the vegetal drug by chromatographic and spectroscopic techniques and to evaluate the antimicrobial activity of the extract. The qualitative phytochemical analysis of the extract showed suggestive results for the presence of alkaloids. Two compounds were isolated and identified: 2-methyl-9H- β -carboline-2-ion, a β -carboline alkaloid obtained for the first time as a natural product and 2',6'-dihydroxy-4'-methoxyacetophenone-2'-O- β -glucoside. In the antimicrobial tests, it was possible to o...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

A new glucosidic iridoid from Isodon rubescens
ABSTRACT One new glucosidic iridoid, 6-O-veratroylbarlerin, was isolated from the chloroform/methanol extract of Isodon rubescens (Hemsl.) H.Hara, Lamiaceae aerial parts, along with the known compounds apigenin and caffeic acid. The structure of the new compound was elucidated on the basis of 1D and 2D NMR experiments and ESI-MS technique. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Validated high performance liquid chromatography for simultaneous determination of stability of madecassoside and asiaticoside in film forming polymeric dispersions
The objective of the work was to validate the high performance liquid chromatography for simultaneous determination of stability of madecassoside and asiaticoside in Centella asiatica (L.) Urb., Apiaceae, extract-loaded film forming polymeric dispersions. High performance liquid chromatography method was validated in five topics: linearity and range, limit of detection and limit of quantitation, specificity, precision, and accuracy. Results showed the method had a good linearity (R2> 0.9990) in the range of 5 – 150 µg/ml and specific. The limit of detection and limit of quantitation of madecassoside were 81 ...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Quantification of major phenolic and flavonoid markers in forage crop Lolium multiflorum using HPLC-DAD
The objective of this study was to perform preliminary screening of phytochemical compounds and quantification of major phenolics and flavonoid markers in Italian ryegrass extract using HPLC-DAD. Previously, LC – MS analysis has identified different phenolic acids, including caffeic acid, ferulic acid, p-coumaric acid, chlorogenic acid, dihydroxy benzoic acid, propyl gallate, catechin, and six flavonoids including rutin hydroxide, luteolin, kaemferol, vitexin, narcissoside, and myricetin from Italian ryegrass extract. In the present study, Italian ryegrass silage powder was extracted with ethanol: water for 20 min at...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Comparative leaf morpho-anatomy of six species of Eucalyptus cultivated in Brazil
ABSTRACT The present work provides a comparative account of the morpho-anatomy of six species of Eucalyptus, namely E. badjensis Beuzev.& Welch, E. benthamii Maiden& Cambage, E. dunnii Maiden, E. grandis W.Hill, E. globulus Labill. and E. saligna Sm., Myrtaceae. Leaf samples of these six species were investigated by light and scanning electron microscopy. The observed microscopic features that can be useful in the identification and quality control of the studied species include the morphology of epicuticular waxes, presence of prismatic crystals on the leaf surface, leaf midrib shape and arrangement of its vascula...
Source: Revista Brasileira de Farmacognosia - July 10, 2018 Category: Drugs & Pharmacology Source Type: research