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Justicia pectoralis, a coumarin medicinal plant have potential for the development of antiasthmatic drugs?
ABSTRACT Justicia pectoralis Jacq., Acanthaceae, is a medicinal plant found Central America. In the Northeast of Brazil, it is popularly known as “ chamb á ” being extensively used in homemade preparations for the treatment of cough, bronchitis and asthma. The species is part of a public phytotherapy program in Brazil entitled “ Farm ácias Vivas ” , National Record of Plants of Interest to the National Health System and the National Formulary of Herbal medicines. This paper aims to critically review the available scientific literature regarding the health promoting effects of J. pector...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Himatanthus drasticus: a chemical and pharmacological review of this medicinal species, commonly found in the Brazilian Northeastern region
In conclusion, although the literature shows several works on species of the Himatanthus genus, including H. drasticus, dealing with some bioactive compounds as triterpenes, translational studies focusing upon the clinical uses of this medicinal species are still in great need. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Chemical constituents from Casearia spp. (Flacourtiaceae/Salicaceae sensu lato)
ABSTRACT Chemical investigation of the leaves of Casearia gossypiosperma Briq., Salicaceae, led to the isolation of two known flavonoids, (+)-taxifolin and quercetin, the leaves of Casearia decandra Jacq. have afforded hydroquinone, the leaves of Casearia rupestris Eichler and Casearia lasiophylla Eichler have afforded a diterpene, (E)-phytol, and the leaves of C. rupestris and Casearia obliqua Spreng. have afforded sitosterol. The twigs of Casearia lasiophylla Eichler led to the isolation of two compounds (+)-pinoresinol, and N-trans-feruloyltyramine, and the twigs of C. obliqua have afforded N-trans-feruloyltyramine, N-t...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Chrysosplenetin, in the absence and presence of artemsininin, alters breast cancer resistance protein-mediated transport activity in Caco-2 cell monolayers using aristolochic acid I as a specific probe substrate
ABSTRACT The present study describes the impact of chrysosplenetin, in the absence and presence of artemisinin, on in vitro breast cancer resistance protein-mediated transport activity in Caco-2 cell monolayers using aristolochic acid I as a specific probe substrate. We observed that novobiocin, a known breast cancer resistance protein active inhibitor, increased Papp (AP-BL) of aristolochic acid I 3.13 fold (p
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Impact of chrysosplenetin, per se or in combination with artemisinin, on breast cancer resistance protein (Bcrp)/ABCG2 mRNA expression levels in mice small intestine
This study, therefore, further addressed the impact of chrysosplenetin, per se or in combination with artemisin, on Bcrp/ABCG2 mRNA expression levels in mice small intestine determined by western blot and real time-quantitative polymerase chain reaction (RT-qPCR) assay. The drugs were intragastrically administrated once per day for 7 days. Novobiocin, a known Bcrp inhibitor, was observed to have no impact on Bcrp/ABCG2 levels with or without artemisinin versus vehicle. Interestingly, artemisinin alone attenuated Bcrp level while chrysosplenetin alone increased it (p
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Relative popularity level of medicinal plants in Talagang, Punjab Province, Pakistan
ABSTRACT This is the first ethnobotanical exploration with the aim to document the traditional medicinal usage of plants with the therapeutic values in Tehsil Talagang of Punjab province, Pakistan. The study shows the dependence of local people on medicinal plants in their daily life and provides practical evidence regarding the traditional usage of medicinal plants in health care practices. A total of 196 respondents including residents of the study area with gender representation and traditional healers were interviewed by using visual appraisal approach and rapid rural appraisal methods along semi-structured interviews ...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Phytochemical composition and chronic hypoglycemic effect of Rhizophora mangle cortex on STZ-NA-induced diabetic rats
ABSTRACT Type 2 diabetes is a major health problem in Mexico, as it is in other countries, is a chronic condition that develops when the body cannot produce enough insulin or cannot use it appropriately. Both insulin deficiency and insulin resistance lead to high blood glucose levels. In Mexico, people with diabetes are known to use the decoction of red mangrove (Rhizophora mangle L., Rhizophoraceae) bark to control blood glucose levels. Therefore, in this study, we sought to investigate the chronic hypoglycemic and hypolipidemic effects of R. mangle; we also elucidate some of the major phytochemical compounds of R. mangle...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro antitubercular activity of extract and constituents from the stem bark of Disthemonanthus benthamianus
ABSTRACT A new C-glycosylflavone, apigenin 7-methyl ether 6-C-[ β -xylopyranosyl-(1 → 3)- β -glucopyranoside] named distemonanthoside was isolated from the stem bark of Distemonanthus benthamianus Baill., Fabaceae, along with six known compounds, sitosterol 3-O- β -D-glucopyranoside, 4-methoxygallic acid, syringic acid, quercetin, 6"-O-acetylvitexin, quercetin 3-O- β -D-glucopyranoside. The structures of those compounds and others were determined through spectral analyses. Compounds distemonanthoside, sitosterol 3-O- β -D-glucopyranoside, 4-methoxygallic acid and quercetin were tested aga...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity
ABSTRACT The metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging th...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Isolation and identification of cytotoxic compounds from a fruticose lichen Roccella montagnei, and it's in silico docking study against CDK-10
ABSTRACT Roccella montagnei B él. belongs to lichen family Roccelleceae growing luxuriantly along the coastal regions of India. As Roccella has been shown to be bioactive, we prepared methanolic extract and assessed its anticancer potential. The methanolic extract showed significant in vitro cytotoxic activity against four huma n cancer cell lines such as colon (DLD-1, SW-620), breast (MCF-7), head and neck (FaDu). This prompted us to isolate bioactive compounds through column chromatography. Two compounds roccellic acid and everninic acid have been isolated, out of which everninic acid is reported for the first tim...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

UPLC/Q-TOF-MS profiling of phenolics from Canarium pimela leaves and its vasorelaxant and antioxidant activities
ABSTRACT Canarium pimela K.D. Koenig, Burseraceae, have a long history of use in the Chinese traditional medicine treatment of various ailments including hypertension, and our research team has reported the anti-hypertensive activity and delineated the mechanism involved in the action. The following research aims to evaluate the vasorelaxant and antioxidant activities of ethanol extract from C. pimela leaves and to analyze its chemical composition by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) that may correlate with their pharmacological activities. The results showe...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Chemical constituents from Bauhinia acuruana and their cytotoxicity
ABSTRACT Phytochemical investigation of Bauhinia acuruana Moric., Fabaceae, resulted in the isolation of sixteen constituents, including two new compounds 2'-hydroxy-2,3,5-trimethoxybibenzyl (1), (2R,3S)-2-(3,4'-dihydroxyphenyl)-5-methoxy-6-methylchroman-3,7-diol (2), together with fourteen known ones (3 – 16). The structures of the compounds were established by spectroscopic analysis including HR-ESI-MS, 1D and 2D NMR data, followed by comparison with previously reported data from the literature. Compounds 1, 2, 6, 7, 8 and 9 were evaluated for their cytotoxicity, which turned out to be marginal in a panel of six hu...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga
In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Plectraterpene, a new ursane-type triterpene ester and other steroids from the aerial parts of Plectranthus montanus
ABSTRACT A new ursane-type triterpene ester, plectraterpene [3 β -(decanoyloxy)-19-hydroxy-urs-12-ene] and four known steroidal compounds have been isolated from the aerial parts of Plectranthus montanus Benth. (syn. Plectranthus cylindraceus Hochst. ex Benth.), Lamiaceae. The known compounds were stigmasterol, sitosteryl ferulate, cholest-5-en-3-O- β -D-glucopyranoside and stigmasterol-3-O- β -D-glucopyranoside. Compounds plectraterpene, sitosteryl ferulate and stigmasterol-3-O- β -D-glucopyranoside are reported for the first time from this plant whereas compound cholest-5-en-3-O- β -D-glucopyrano...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous HPLC analysis of crebanine, dicentrine, stephanine and tetrahydropalmatine in Stephania venosa
ABSTRACT Stephania venosa (Blume) Spreng., Menispermaceae, has been traditionally used as tonic drug and treatment of various diseases in South East Asian countries. In order to evaluate the quality and standardization of S. venosa roots, the HPLC method for quantification of the content of major components in S. venosa was developed and validated. The chromatographic separation was performed on a Hypersil BDS C18 column using gradient system of 100 mM ammonium acetate in water and methanol with flow rate 1 ml/min. Detection wavelength was set at 210 nm for tetrahydropalmatine, 280 nm for dicentrine and crebanine, and 270 ...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Influence of growth regulators on distribution of trichomes and the production of volatiles in micropropagated plants of Plectranthus ornatus
ABSTRACT The profile of volatile organic compounds, the glandular and non-glandular trichomes of Plectranthus ornatus, obtained by in vitro cultivation, was evaluated in plants grown in Murashide and Skoog medium supplemented with benylaminopurine at 4.5, 9.0, and 18.0 µM + naphthaleneacetic acid at 5.37 µM, kinetin at 4.7, 9.3 and 18.5 µM + naphthaleneacetic acid (5.37 µM) or Murashide and Skoog 0 medium (as a control). Scanning Electron Microscopy was performed on samples of the third leaf node of the 90 days old plants obtained from treatment with 4.5 or 9. 0 µM benylaminopurine, and 4.7 or...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Leaf anatomy of Protium ovatum and its antiproliferative potential in cervical cells
ABSTRACT The aim of this study was to analyze the morphology and anatomy of the leaves of Protium ovatum Engl., Burseraceae, and verify the antiproliferative activity in cervical cells. For anatomical analysis, the leaf samples were fixed in formol, acetic acid, alcohol 70, dehydrated, included in hydroxyethyl methacrylate and sectioned at a thickness of 5 – 10 µm in rotative microtome. The samples were stained with toluidine blue and blades mounted with synthetic resin “ Entellan ” . Histochemical tests and scanning electron microscopy were also performed. To investigate the antiproliferative effec...
Source: Revista Brasileira de Farmacognosia - January 11, 2018 Category: Drugs & Pharmacology Source Type: research

Vouacapane diterpenoids isolated from Pterodon and their biological activities
Abstract The Pterodon genus comprises two native species in Brazil, known as “ sucupira-branca ” or “ faveira ” . Their fruits have long been used in Brazilian natural medicine, mainly for the treatment of infections and inflammations. The pharmacological properties of these fruits have often been linked with vouacapane diterpenoids. This review evaluated the scientific research in the period from 1973 to February 2017, aiming to answer how difficult it still is to develop a scientifically supported product based on Pterodon vouacapanes. Therefore, this paper reviews purification, identification, an...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

An overview of dermatological and cosmeceutical benefits of Diospyros kaki and its phytoconstituents
The objective of this article is to present cumulative data on potential use of D. kaki for its possible role in dermatologic and cosmetic applications. Scientific data has revealed an excellent position of D. kaki in both dermatology and cosmetic discipline making it a valuable choice in respective field. Active principles from different plant parts have shown to possess anti-inflammatory, antiallergic, photo-protective, and anti-wrinkle effects with appreciable activities against tyrosinase, elastase, and collagenase enzymes. Promising antioxidant activity and skin whitening potential, augmented by reduction in sebum con...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus
Abstract This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrile:water-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise, accurate and robust. The response was linear over a range of 5 – 200 µg/ml (r> 0.999). The range of recoveries was 92.27 – 102.54%. The relative standard deviation values for intra- and inter-day prec...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Iridoids from leaf extract of Genipa americana
Abstract Genipa americana L., Rubiaceae, is a plant native from Brazil popularly known as “ jenipapo ” . Two iridoids, 1-hydroxy-7-(hydroxymethyl)-1,4aH,5H,7aH-cyclopenta[c]pyran-4-carbaldehyde (1), and iridoid 7-(hydroxymethyl)-1-methoxy-1H,4aH,5H,7aH-cyclopenta[c]pyran-4-carbaldehyde (2) were isolated and identified in the leaf extract of G. americana. Compounds 1 and 2 were identified for the first time in G. americana, and 1 has not been yet described in literature. These substances were analyzed by spectroscopic techniques such as infrared, high resolution mass spectrometry, 1H and 13C 1D; as well as 2D nu...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Sedative and muscle relaxant activities of diterpenoids from Phlomidoschema parviflorum
Abstract Phlomidoschema parviflorum (Benth.) Vved. (Basionym: Stachys parviflora Benth.) Lamiaceae, have significance medicinal importance as it is used in number of health disorders including diarrhea, fever, sore mouth and throat, internal bleeding, weaknesses of the liver and heart genital tumors, sclerosis of the spleen, inflammatory tumors and cancerous ulcers. The present contribution deals with the sedative and muscle relaxant like effects of diterpenoids trivially named stachysrosane and stachysrosane, isolated from the ethyl acetate soluble fraction of P. parviflorum. Both compounds (at 5, 10 and 15 mg/kg, i.p) we...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Punica granatum suppresses colon cancer through downregulation of Wnt/β-Catenin in rat model
This study aims to elucidate the beneficial effect of Punica granatum L., Lythraceae (pomegranate) peel extract in the management of colon cancer induced intrarectally with N-methylnitrosourea. Adult male Sprague-Dawley rats were administered N-methylnitrosourea (2 mg in 0.5 ml water/rat) intrarectally three times/week for five weeks to induce colorectal cancer, followed by treatment with either 5-fluorouracil (12.5 mg/kg, i.p.) or Punica peel extract (2.25 or 4.5 g/kg, p.o.). Developed tumor elevated plasma TGF- β , and Bcl2, serum epidermal growth factor, carcinoembryonic antigen, colon cancer specific antigens, and...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Isovitexin as marker and bioactive compound in the antinociceptive activity of the Brazilian crude drug extracts of Echinodorus scaber and E. grandiflorus
Abstract Echinodorus scaber Rataj and Echinodorus grandiflorus (Cham.& Schltdl.) Micheli, Alismataceae, are popularly used to relieve inflammatory complaints and as diuretic. A study on the antinociceptive effect and selected marker compounds in eleven extracts from different locations was undertaken and their antinociceptive effect was assessed. The fingerprints were compared by HPLC-DAD and the content of vitexin, isovitexin, isoorientin and vitexin-2-O-rhamnoside were determined. All samples presented antinociceptive activity reducing the writhes by 36.4-62.5% and 47.4-79.8% at 10 and 50 mg/kg, respectively; indomet...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Anti-caries activity of selected Sudanese medicinal plants with emphasis on Terminalia laxiflora
Abstract In Sudan, some medicinal plants, such as Acacia seyal, Calotropis procera and Balanites aegyptiaca have been used to prevent or treat oral health problems. The stem and stem bark of Terminalia laxiflora Engl., Combretaceae, are used as antiseptics for mouthwash to prevent gingivitis and thrush in Africa. Methanol and 50% hydroethanolic extracts of 25 plants that are used in traditional Sudanese medicine for several diseases and cavity disorders were screened for anti-cavity activities. T. laxiflora methanolic wood extracts, which exhibited such activity, were investigated. The crude extracts were assayed for their...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Short-term carcinogenesis evaluation of Casearia sylvestris
In this study, we proposed a short-term carcinogenesis evaluation of C. sylvestris in Wistar rats, aiming to check the safety of this species to use it as proposed by Brazilian Unified Health System. C. sylvestris leaves were obtained and the crude extract was prepared by maceration from methanol/water. Wistar rats were orally treated for 12 weeks with 50, 250 or 500 mg kg − 1 of crude extract or vehicle. Body weight, daily morbidity and mortality were monitored. Blood and bone marrow samples were collect for micronucleus test, comet assay and tumor markers evaluation. Vital organs were removed to macro and histopath...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Anti-adhesion potential of non-polar compounds and extracts from Ficus natalensis
Abstract Four triterpenoids, ergosta-4,6,8(14),22-tetraene-3-one 1, stigma-4-ene-3-one 2, 3 β -hydroxy-21 β -H-hop-22(29)-ene 3, sitosterol and a quinone, tectoquinone 4, were isolated from the leaf, stem bark and fruit extracts of Ficus natalensis subsp. natalensis, Moraceae, a medicinal fig found in Africa. The pure compounds 1 – 4 and crude extracts were tested for their antibacterial activity against five Gram-negative and seven Gram-positive strains and for their potential anti-biofilm activity. Antimicrobial susceptibility was observed with all pure compounds tested at 250 µg against the majorit...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Antiproliferative effects of pinostrobin and 5,6-dehydrokavain isolated from leaves of Alpinia zerumbet
This study was designed to identify the chemical constituents of hydroalcoholic and dichloromethane extracts from A. zerumbet leaves and to investigate their in vitro antiproliferative activity. The isolated phytochemicals included kaempferol, dihydro-5,6-dehydrokavain, 5,6-dehydrokavain, and pinostrobin. The hydroalcoholic extract inhibited cellular proliferation only at high concentrations, while the dichloromethane extract showed a moderate antiproliferative effect against leukemia and lung tumor cell lines. 5,6-Dehydrokavain showed potent cytostatic activity against glioblastoma cells and a moderate effect on all other...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Antinociceptive activity of Cistanche salsa stolons, growing in the Republic of Kazakhstan
The objective of this study was to evaluate the antinociceptive effect of the hydroethanol extract of Cistanche salsa (C.A.Mey.) Beck, Orobanchaceae, stolons in animal models of pain. Chemical composition of Herba Cistanche was analyzed by HPLC-UV. Mice Swiss Webster (25 – 30 g, n = 6) were orally pre-treated with Herba Cistanche (10, 30 or 100 mg/kg) and evaluated in the formalin test and in the capsaicin- or glutamate-induced licking response. Kazakh Herba Cistanche is composed mainly by phenylpropanoid glycosides, from which echinacoside, acteoside and tubuloside B are the main constituents. When Herba Cistanche w...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

GC–MS based metabolite profiling and angiotensin I-converting enzyme inhibitory property of black tea extracts
Abstract Angiotensin I-converting enzyme inhibitors are used as therapeutic agents for the treatment of hypertension. Regular consumption of black tea (Camellia sinensis (L.) Kuntze, Theaceae) has been reported to lower blood pressure. The aims of the present work were to compare chemical composition and angiotensin I-converting enzyme inhibitory properties of infusion and decoction of four samples of black tea. GC/MS based metabolomics approach helped in identification of fifty-one metabolites including ten organic acids, one inorganic acid, sixteen amino acids, two sugars, five sugar alcohols, fifteen phenols and flavono...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Identification of phenolic compounds in Myricaria bracteata leaves by high-performance liquid chromatography with a diode array detector and liquid chromatography with tandem mass spectrometry
This study shows that M. bracteata leaves are a source of flavonoids with possible biological activities. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Gamma radiation treatment activates glucomoringin synthesis in Moringa oleifera
Abstract Plants are a very rich source of pharmacologically relevant metabolites. However, the relative concentrations of these compounds are subject to the genetic make-up, the physiological state of the plant as well as environmental effects. Recently, metabolic perturbations through the use of abiotic stressors have proven to be a valuable strategy for increasing the levels of these compounds. Oxidative stress-associated stressors, including ionizing radiation, have also been reported to induce metabolites with various biological activities in plants. Hence, the aim of the current study was to investigate the effect of ...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Perillanolides A and B, new monoterpene glycosides from the leaves of Perilla frutescens
Abstract Two new monoterpene glycosides, perillanolides A and B, together with a known compound reported from the genus Perilla for the first time were isolated and characterized from the leaves of Perilla frutescens (L.) Britton, Lamiaceae, a garnish and colorant for foods as well as commonly used for traditional medicine. The structures of the isolated compounds were elucidated on the basis of extensive spectroscopic evidences derived from nuclear magnetic resonance experiments, mass spectrometry and by comparing their physical and spectroscopic data of literature. These compounds, together with the previously isolated s...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Chemical identification and quantification of Hu-Gu capsule by UHPLC–Q-TOF-MS and HPLC-DAD
Abstract Hu-Gu capsule is a traditional Chinese medical formula for preventing glucocorticoid-induced osteoporosis. In this work, an ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry was developed for chemical identification of the phytochemical compositions in positive ion mode. A total of 41 compounds were detected in Hu-Gu capsule, and 25 compounds were characterized and identified by retention time, accurate mass within 5 ppm error and characteristic fragment ions. Among of them, four major flavonoids were further quantified by high performance...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Yeast extract elicitation increases vinblastine and vincristine yield in protoplast derived tissues and plantlets in Catharanthus roseus
In this study, the yeast extract elicitation on vinblastine and vincristine was studied in various protoplast derived tissues and plantlets. Four different yeast extract treatments (T1 = 0.5 g/l, T2 = 1.0 g/l, T3 = 1.5 g/l and T4 = 2.0 g/l) were prepared and used. The alkaloid was quantified and a comparative account of yield were presented by the use of High performance thin layer chromatography. The yeast extract amendment in medium improved vinblastine and vincristine yield in cultivating tissues, maximum being in germinating embryos and in in vitro raised leaf. The highest yield was in T3 (1.5 mg/l) in which 22.74% vin...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Anatomical characterization of ultra-structures, biominerals and histolocalization of metabolites in leaves of Genipa americana
Abstract Inserted in one of the largest families among the Angiosperms, Genipa americana L., Rubiaceae, can be found in all Brazilian territory, presenting great medicinal importance, where several uses have been attributed. In view of this, this work has the purpose of analyzing the ultrastructural, biomineral, phytochemical and histochemical characteristics of the leaves of this native species from Brazil. For this, light microscopy, polarization and scanning electron microscopy techniques were used with X-ray scattering energy, associated to chromatographic and histochemical tests. The anatomical ultrastructural charact...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Pharmacological potential of Maytenus species and isolated constituents, especially tingenone, for treatment of painful inflammatory diseases
ABSTRACT Uses of medicinal plants by people around the world significantly contribute and guide biologically active compounds research that can be useful in the combat against various diseases. Due to a great chemical and structural variety found in their vegetal structures it consolidates ethnopharmacology as an important science for the pharmaceutical section. Inserted in the diversity of medicinal plants, is the Maytenus genus, whose research has already revealed lots of isolated substances which are responsible for a great variety of biological activities, among which we cite analgesic and anti-inflammatory, for the tr...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Aflatoxins produced by Aspergillus nomius ASR3, a pathogen isolated from the leaf-cutter ant Atta sexdens rubropilosa
ABSTRACT Aspergillus spp. cause economic impacts due to aflatoxins production. Although the toxicity of aflatoxins is already known, little information about their ecological roles is available. Here we investigated the compounds produced by Aspergillus nomius ASR3 directly from a dead leaf-cutter queen ant Atta sexdens rubropilosa and the fungal axenic culture. Chemical analyses were carried out by high-resolution mass spectrometry and tandem mass spectrometry techniques. Aflatoxins B1 and G1 were detected in both the axenic culture and in the dead leaf-cutter queen ant. The presence of these mycotoxins in the dead leaf-c...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

In vitro callogenesis of Poincianella pyramidalis (catingueira)
ABSTRACT This work describes the establishment of procedures to induce in vitro callogenesis from Poincianella pyramidalis (Tul.) L. P. Queiroz, Fabaceae, explants. Nodal, internodal and leaf segments were isolated from in vitro germinated seedlings and cultured in MS medium with 0, 2.5, 5 and 10 mg l-1 of 2,4-dichlorophenoxyacetic acid. After 30 days, the explants with induced callus showed a quadratic response for the segments nodal, internodal and leaf, with increasing the callus formation in 2,4-dichlorophenoxyacetic acid concentrations of 6.28, 6.49 and 4.91 mg l-1, respectively. In 30 days there was a linear oxidatio...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Box–Behnken experimental design for extraction of artemisinin from Artemisia annua and validation of the assay method
The objective of this study was to evaluate the effects of the extraction parameters (ethanol graduation, previous shaking time in an ultrasound bath and drug/solvent ratio) on the yield of artemisinin in the liquid extract obtained by percolation from A. annua and then optimize the extraction efficiency of this compound. The Box – Behnken (33) factorial design was used in association with response surface methodology. The derivatization reaction of artemisinin was used in an analytical method which was submitted to validation, after reaching the specification of the selectivity, linearity, precision, accuracy and ro...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Turbo-extraction of glycosides from Stevia rebaudiana using a fractional factorial design
ABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, leaf extract has recently called the attention of food industry as a proposal for natural sweetener. The sweet flavor is attributed to the glycosides, in especial stevioside and rebaudioside A, which are the plant main chemical markers. The aim of the work reported here was to optimize the turbo-extraction of stevia leaves using water, ethanol 70% and 90% (w/w) as green solvents. A 25-2 factorial design was applied to study the linear effects of the drug size, solvent to drug ratio, temperature, time and also the turbolysis speed on the extraction of glycosides. The...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Development and validation of a stability indicating method for quantification of the sesquiterpene lactone eremantholide C from Lychnophora trichocarpha (Brazilian arnica)
ABSTRACT Pharmacological activities as anti-inflammatory, anti-hyperuricemic, anti-gouty arthritis, antitumor and trypanocidal activities of the aerial parts from Lychnophora trichocarpha (Spreng.) Spreng. ex Sch.Bip., Asteraceae (Brazilian arnica) have already been proved. Eremantholide C is a sesquiterpene lactone and one of the active chemical constituents responsible for these activities presented by L. trichocarpha. Therefore, the aim of this work was to develop and validate a stability indicating HPLC method for eremantholide C. Eremantholide C stability was evaluated in L. trichocarpha ethanolic extract and in its i...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Anti-inflammatory action of ethanolic extract and clerodane diterpenes from Casearia sylvestris
ABSTRACT The present study aimed to investigate the anti-inflammatory activity of ethanolic extract from Casearia sylvestris Sw., Salicaceae, leaves and to identify the compounds responsible for this activity. The ethanolic extract from C. sylvestris leaves was fractionated by solid phase extraction and the chemical composition of extract and fractions were assessed by chromatographic techniques. Casearin-like clerodane diterpenes were quantified in ethanolic extract (27.4%, w/w) and in fraction 2 of solid phase extraction (50.6%, w/w). Carrageenan-induced paw edema and carrageenan-induced pleurisy assays (rats) were used ...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Comparative study of the hypocholesterolemic, antidiabetic effects of four agro-waste Citrus peels cultivars and their HPLC standardization
This study was aimed to explore the hypocholesterolemic, antidiabetic activities of four varieties of citrus peels agrowastes, to isolate the main flavonoids in the active fractions and to quantify them by HPLC method for nutraceutical purposes. All the tested samples of the agro-waste Citrus fruits peels showed significant decrease in cholesterol, triacylglyceride and glucose. The most decrease in cholesterol level was observed by mandarin peels aqueous homogenate and its hexane fraction (59.3% and 56.8%, respectively) reaching the same effect as the reference drug used (54.7%). Mostly, all samples decrease triacylglyceri...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

α-Glucosidase and pancreatic lipase inhibitory activities and glucose uptake stimulatory effect of phenolic compounds from Dendrobium formosum
This study is the first report on the phytochemical constituents and anti-diabetic and anti-obesity activities of D. formosum. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Cytotoxic diterpenoids from the roots of Salvia lachnocalyx
In conclusion, compounds 2 and 3 are presented as compounds that deserve further investigation of their biological activities. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Maytenus distichophylla and Salacia crassifolia: source of products with potential acetylcholinesterase inhibition
ABSTRACT The phytochemical study of the extract leaves from Maytenus distichophylla Mart. and Salacia crassifolia (Mart. ex Schult.) G. Don, Celastraceae, resulted in the isolation of 3-oxofriedelane, 3 β -hydroxyfriedelane, 3 β ,24-dihydroxyfriedelane, 3-oxo-28,29-dihydroxyfriedelane, two mixtures of pentacyclic triterpenes ( α -amyrin with β -amyrin and 3 β -stearyloxy-urs-12-ene with 3 β -stearyloxy-olean-12-ene), 3 β -palmityloxy-urs-12-ene, the steroid β -sitosterol and its glycosylated derivative β -glucosyl- β -sitosterol, tritriacontanoic acid and the natural polym...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1 .8-fold), red (15.4...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Determination of phenolic profile by HPLC-ESI-MS/MS and anti-inflammatory activity of crude hydroalcoholic extract and ethyl acetate fraction from leaves of Eugenia brasiliensis
This study investigated the anti-inflammatory activity and phenolic profile of the crude hydroalcoholic extract and ethyl acetate fraction from E. brasiliensis leaves. Crude hydroalcoholic extract and the ethyl acetate fraction were analyzed by HPLC-ESI-MS/MS in comparison to standard phenolic compounds. The anti-inflammatory activity of the crude hydroalcoholic extract (1, 10 and 25 mg kg-1) and the ethyl acetate fraction (10, 25 and 50 mg kg-1) was evaluated in a swiss mouse model of acute pleurisy induced by carrageenan, being the total cell count, exudation and analysis of nitrite/nitrate the inflammation parameters. H...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research

Phytochemical study of Pilosocereus pachycladus and antibiotic-resistance modifying activity of syringaldehyde
ABSTRACT Pilosocereus pachycladus F. Ritter, Cactaceae, popularly known as "facheiro", is used as food and traditional medicine in Brazilian caatinga ecoregion. The plant is used to treat prostate inflammation and urinary infection. The present work reports the first secondary metabolites isolated from P. pachycladus. Therefore, the isolated compound 4-hydroxy-3,5-dimethoxy benzaldehyde (syringaldehyde) was evaluated as modulator of Staphylococcus aureus pump efflux-mediated antibiotic resistance. The isolation of compounds was performed using chromatographic techniques and the structural elucidation was carried ...
Source: Revista Brasileira de Farmacognosia - September 4, 2017 Category: Drugs & Pharmacology Source Type: research