Structural and functional characterization of a maltose/maltodextrin ABC transporter comprising a single solute binding domain (MalE) fused to the transmembrane subunit MalF
Publication date: Available online 4 September 2018Source: Research in MicrobiologyAuthor(s): Anke Licht, Martin Bommer, Tobias Werther, Kristin Neumann, Carolin Hobe, Erwin SchneiderAbstractCanonical ATP-binding cassette import systems rely on extracellular substrate binding proteins (SBP) for function. In gram-negative bacteria, SBPs are usually freely diffusible in the periplasm and, where studied, exist in excess over their cognate transporters. However, in vitro studies with the maltose transporter of Escherichia coli (MalFGK2) have demonstrated that mechanistically one copy of its SBP (MalE) per transport complex is ...
Source: Research in Microbiology - September 5, 2018 Category: Microbiology Source Type: research
In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25
Publication date: Available online 1 September 2018Source: Research in MicrobiologyAuthor(s): Li-Zhu Liu, Zhen-Yuan Nie, Yi Yang, Xuan Pan, Xu Xia, Yu-Hang Zhou, Jin-Lan Xia, Li-juan Zhang, Xiang-jun Zhen, Hong-ying YangAbstractFor the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0,...
Source: Research in Microbiology - September 2, 2018 Category: Microbiology Source Type: research
Spatial and Seasonal Bacterioplankton Community Dynamics in the Main Channel of the Middle Route of South-to-North Water Diversion Project
In this study, the spatial and seasonal bacterioplankton community dynamics were investigated in the main channel of the Middle Route of the South-to-North Water Diversion Project (MRP) using Illumina HiSeq sequencing. Water samples were collected in spring and summer from south to north at eight water quality monitoring stations, respectively. The results showed that seasonal changes had a more pronounced effect on the bacterioplankton community compositions (BCCs) than spatial variation. The diversity analysis results indicated that samples of summer have more operational taxonomic units (OTUs), higher richness and diver...
Source: Research in Microbiology - September 2, 2018 Category: Microbiology Source Type: research
Making sticky cells: effect of galactose and ferrous iron on the attachment of Leptospirillum ferrooxidans to mineral surfaces
Publication date: Available online 1 September 2018Source: Research in MicrobiologyAuthor(s): Paulina Aguirre, Karlo Guerrero, Aminael Sánchez-Rodríguez, Juan Carlos Gentina, Axel SchippersAbstractThe purpose of this study was to compare the efficacy of galactose and high initial ferrous iron concentrations as inducers for extracellular polymeric substances (EPS) production in planktonic cells of Leptospirillum ferrooxidans and to study cell attachment to a mineral surface in comparison to cells not exposed to such substances. Leptospirillum ferrooxidans was successfully adapted to grow in a modified 9K medium at differe...
Source: Research in Microbiology - September 2, 2018 Category: Microbiology Source Type: research
LmrP from Lactoccoccus lactis: a tractable model to understand secondary multidrug transport in MFS
Publication date: Available online 23 August 2018Source: Research in MicrobiologyAuthor(s): Aurélie Roth, Cedric GovaertsAbstractThe secondary transporter LmrP from L. lactis is a remarkable model to study the molecular basis of secondary multidrug transport. This review article addresses more than twenty years of research about transport activity, substrates range, conformational dynamics and mechanistic models of drug export for LmrP. Several studies have shown that the transporter alternates between inward-open and outward-open conformations and that the transition is regulated by the protonation state of key acidic re...
Source: Research in Microbiology - August 24, 2018 Category: Microbiology Source Type: research
Insights into the biology of acidophilic members of the Acidiferrobacteraceae family derived from comparative genomic analyses
Publication date: Available online 22 August 2018Source: Research in MicrobiologyAuthor(s): Francisco Issotta, Ana Moya-Beltrán, Cristóbal Mena, Paulo C. Covarrubias, Christian Thyssen, Sören Bellenberg, Wolfgang Sand, Raquel Quatrini, Mario VeraAbstractThe family Acidiferrobacteraceae (order Acidiferrobacterales) currently contains Gram negative, neutrophilic sulfur oxidizers such as Sulfuricaulis and Sulfurifustis, as well as acidophilic iron and sulfur oxidizers belonging to the Acidiferrobacter genus. The diversity and taxonomy of the genus Acidiferrobacter has remained poorly explored. Although several metagenome a...
Source: Research in Microbiology - August 23, 2018 Category: Microbiology Source Type: research
Analysis of desferrioxamine-like siderophores and their capability to selectively bind metals and metalloids: Development of a robust analytical RP-HPLC method
Publication date: Available online 20 August 2018Source: Research in MicrobiologyAuthor(s): Ringo Schwabe, Marlene Kirstin Anke, Katarzyna Szymańska, Oliver Wiche, Dirk TischlerAbstractThe Actinobacterium Gordonia rubripertincta CWB2 (DSM 46758) produces hydroxamate-type siderophores (188 mg L-1) under iron limitation. Analytical reversed-phase HPLC allowed determining a single peak of ferric iron chelating compounds from culture broth which was confirmed by the Fe-CAS assay. Elution profile and its absorbance spectrum were similar to those of commercial (des)ferrioxamine B which was used as reference compound. This confi...
Source: Research in Microbiology - August 20, 2018 Category: Microbiology Source Type: research
Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex
In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius. Among A. caldus strains and isolates this provirus had a modest prevalence (30%). Comparative genomic analysis revealed a significant conservation with the T. tepidarius AcaML1-like provirus, excepting the tail genes, and a high conservati...
Source: Research in Microbiology - August 20, 2018 Category: Microbiology Source Type: research
Characterization of a lytic vibriophage VP06 of Vibrio parahaemolyticus
Publication date: Available online 2 August 2018Source: Research in MicrobiologyAuthor(s): Hin-chung Wong, Tzu-Yun Wang, Chu-Wen Yang, Chung-Tao Tang, Chingwen Ying, Chun-Hsiung Wang, Wei-Hau ChangAbstractVibrio parahaemolyticus is a human enteropathogenic bacterium and is also pathogenic to shrimp and finfish. In a search for a biocontrol agent for V. parahaemolyticus and other pathogenic Vibrio species, a lytic phage VP06 was isolated from oyster using V. parahaemolyticus as the host. VP06 is a Siphoviridae phage with a polyhedral head and a long tail. The genome sequence of VP06 was 75,893 nucleotides in length and the ...
Source: Research in Microbiology - August 3, 2018 Category: Microbiology Source Type: research
Editorial board
Publication date: July–August 2018Source: Research in Microbiology, Volume 169, Issue 6Author(s): (Source: Research in Microbiology)
Source: Research in Microbiology - July 19, 2018 Category: Microbiology Source Type: research
The significance of pH in dictating the relative toxicities of chloride and copper to acidophilic bacteria
Publication date: Available online 19 July 2018Source: Research in MicrobiologyAuthor(s): Carmen Falagán, D. Barrie JohnsonAbstractThe ability of acidophilic bacteria to grow in the presence of elevated concentrations of cationic transition metals, though varying between species, has long been recognized to be far greater than that of most neutrophiles. Conversely, their sensitivity to both inorganic and organic anions, with the notable exception of sulfate, has generally been considered to be far more pronounced. We have compared the tolerance of different species of mineral-oxidizing Acidithiobacillus and Sulfobacillus,...
Source: Research in Microbiology - July 19, 2018 Category: Microbiology Source Type: research
Quantitative proteomics using SWATH-MS identifies mechanisms of chloride tolerance in the halophilic acidophile Acidihalobacter prosperus DSM 14174
In this study, the differential protein expression of the acidophilic halophile, Acidihalobacter prosperus DSM 14174 (strain V6) was studied with the aim of understanding its mechanisms of tolerance to high chloride ion stress in the presence of low pH, using Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). In acidophiles, chloride stress results in both osmotic stress as well as acidification of the cytoplasm due to the ability of chloride to permeate the cell membrane and disrupt the reversed transmembrane potential which normally extrudes protons. The proteomic response of Ac. prosperus DSM 1417...
Source: Research in Microbiology - July 19, 2018 Category: Microbiology Source Type: research
Characterization of a luxI/luxR-type quorum sensing system and N-acyl homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in Serratia plymuthica RVH1
Publication date: Available online 14 January 2007Source: Research in MicrobiologyAuthor(s): Rob Van Houdt, Pieter Moons, Abram Aertsen, An Jansen, Kristof Vanoirbeek, Mavis Daykin, Paul Williams, Chris W. MichielsThe Publisher regrets that this article was an accidental duplication of an article that has already been published in Res Microbiol, 158 (2007) 150-158, doi: 10.1016/j.resmic.2006.11.008. The duplicate article has therefore been withdrawn. (Source: Research in Microbiology)
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
Corrigendum to “The microbiome of a striped dolphin (Stenella coeruleoalba) stranded in Portugal” [Res Microbiol (2017) 85–93]
Publication date: Available online 29 January 2017Source: Research in MicrobiologyAuthor(s): Filipa Godoy-Vitorino, Arnold Rodriguez-Hilario, Ana Luísa Alves, Filipa Gonçalves, Beatriz Cabrera-Colon, Cristina Sousa Mesquita, Pedro Soares-Castro, Marisa Ferreira, Ana Marçalo, José Vingada, Catarina Eira, Pedro Miguel Santos (Source: Research in Microbiology)
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
The fascinating but mysterious mechanistic aspects of multidrug transport by MdfA from Escherichia coli
Publication date: Available online 23 September 2017Source: Research in MicrobiologyAuthor(s): Eliane H. Yardeni, Elia Zomot, Eitan BibiAbstractMdfA is an interesting member of a large group of secondary multidrug (Mdr) transporters. Through genetic, biochemical and biophysical studies of MdfA, many challenging aspects of the multidrug transport phenomenon have been addressed. This includes its ability to interact with chemically unrelated drugs and how it utilizes energy to drive efflux of compounds that are not only structurally, but also electrically, different. Admittedly, however, despite all efforts and a recent pion...
Source: Research in Microbiology - July 10, 2018 Category: Microbiology Source Type: research
