Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes
ConclusionOocyte dysmorphisms did not seem to affect survival, fertilization and embryo development in vitrified autologous oocytes, and yielded comparable results to the morphologically normal oocytes. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 9, 2019 Category: Reproduction Medicine Source Type: research

Consultation and Treatment Behavior of Infertile Couples in China: a population-based study
ConclusionWe conclude that the prevalence of infertility in China is high, but the uptake of treatment was relatively low. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 9, 2019 Category: Reproduction Medicine Source Type: research

Evaluation of next-generation sequencing–based preimplantation genetic diagnosis for balanced reciprocal translocation carriers
ConclusionsCNV-Seq-based NGS is an efficient and reliable PGD method for balanced reciprocal translocation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

Maternal β-hCG levels in early IVF pregnancy: association with the embryo development stage of blastocyst
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Wenyue Zhu, Queenie Yeung, David Chan, Ling Chi, Jin Huang, Qiong Wang, Jacqueline Chung, TC LiAbstractResearch QuestionDoes the development stage of the blastocyst on embryo transfer (ET) day affect the serum β-human chorionic gonadotropin (β-hCG) level 9 days and 16 days after embryo transfer in women whose pregnancy progressed to beyond 13 weeks?DesignThe retrospective study involved 204 cases whose pregnancy progressed to beyond 13 weeks of single blastocyst transfer in fresh (n=66) or frozen (n=138) cycles in a...
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

The high-risk human Papillomavirus continuum along the female reproductive tract and its relationship to infertility and endometriosis
ConclusionsThis study demonstrate that there is an association between hrHPV infections in the UGT with infertility, particularly with endometriosis. It also show a possible continuum hrHPV infection, from LGT to UGT. It suggest future larger studies that could fully investigate the role of hrHPV as an eventual cause of endometriosis and infertility. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

A multicenter evaluation of the Elecsys® AMH immunoassay for prediction of antral follicle count
ConclusionThe Elecsys® AMH immunoassay provides a robust and fully automated method to measure serum AMH levels. Women with AMH values below the cut-off of 1.77 ng/mL are unlikely to have AFC>15. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

Does Polycystic Ovary Syndrome Associate with Risk of Female Sexual Dysfunction? A Systematic Review and Meta-analysis
This study aims to evaluate whether polycystic ovary syndrome (PCOS) is a risk factor for female sexual dysfunction (FSD) through a meta-analysis. The association between PCOS and risk of FSD was performed by using the relative risk (RR) or the standard mean differences (SMD) with a 95% confidence interval (CI). The protocol for this meta-analysis is available from PROSPERO (CRD42018102247). Overall, 2626 participants (mean age: 25-36 years) were included from 10 studies (5 cross-sectional and 5 case-control studies), 1163 of whom were women with PCOS. The pooled results from 8 included studies providing the number of case...
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

Preconditioning of sperm with sublethal nitrosative stress: a novel approach to improve frozen–thawed sperm function
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Maryam Hezavehei, Homa Mohseni Kouchesfahani, Abdolhossein Shahverdi, Mohsen Sharafi, Ghasem Hosseini Salekdeh, Poopak Eftekhari-YazdiAbstractResearch questionCan sublethal stress induced by nitric oxide on fresh human spermatozoa protect the functional properties of post–thaw human spermatozoa?DesignSemen samples were obtained from 46 donors. Twenty semen samples were used to determine toxicity level of nitric oxide by incubation of semen with different concentrations of nitric oxide (0.01 to 400 μM). Then, 26 semen...
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

Beneficial effects of dietary nitrate on testicular injury by improving glycemia and inhibiting apoptosis in Streptozotocin-induced diabetic male rats
ConclusionsWe demonstrated that treatment with sodium nitrate could modulate the apoptosis which is a major cause for the diabetic testicular disorder. These experiments suggest that NO plays an important role in the function of the reproductive system.Graphical abstract (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

“Is blood thicker than water?” Donor conceived offspring subjective experiences of the donor: A systematic narrative review
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Canzi Elena, Accordini Monica, Facchin FedericaAbstractThe aim of this study was to provide a systematic narrative review of the published research articles on donor conceived (DC) offspring's subjective experiences of their donor. A systematic search of electronic databases provided 29 published quantitative and qualitative studies matching our inclusion criteria. The analysis of the studies outcomes led to the identification of four main themes: a) views/representations of the donor; b) desire to contact the donor (in terms...
Source: Reproductive BioMedicine Online - January 8, 2019 Category: Reproduction Medicine Source Type: research

Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model
ConclusionThe three-dimensional levitation culture system improves the viability of in-vitro development of bovine secondary follicles, antrum formation and lower extrusion and degeneration rates and adequate growth rate leading to relevant oocyte viability and meiotic resumption after in-vitro maturation. This approach does not require a specific medium, and has the potential as an alternative method to in-vitro follicle culture in several species, including humans. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 5, 2019 Category: Reproduction Medicine Source Type: research

C-type natriuretic peptide regulates sperm capacitation by the cGMP/PKG signalling pathway via Ca2+ influx and tyrosine phosphorylation
Publication date: Available online 5 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Kejia Wu, Chunlei Mei, Yao Chen, Lidan Guo, Yuejin Yu, Donghui HuangAbstractResearch questionWhat is the effect of C-type natriuretic peptide (CNP) on human sperm capacitation in vitro and what is the mechanism of this effect?DesignCNP/NPR-B expression in the female rat genital tract was examined by immunohistochemistry and western blot assay, and then the role of CNP in human sperm capacitation was determined. The signal transduction pathway of CNP in the process was determined to elucidate the regulation mechanism of CNP by...
Source: Reproductive BioMedicine Online - January 5, 2019 Category: Reproduction Medicine Source Type: research

Obstetric and perinatal outcomes of pregnancies according to initial maternal serum HCG concentrations after vitrified–warmed single blastocyst transfer
ConclusionsLower initial maternal serum HCG levels indicated poorer clinical outcomes. Within the high HCG group, a lower initial maternal HCG level was found to be associated with GDM occurrence and proportion of female neonates. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 5, 2019 Category: Reproduction Medicine Source Type: research

Autologous endometrial cell co-culture improves human embryo development to high-quality blastocysts: a randomized controlled trial
ConclusionThis study demonstrated the benefits of AECC on blastocyst quality compared with conventional embryo culture medium, in a broader category of patients referred to ART as opposed to other studies that concentrated on specific causes of infertility only. However, limitations of the study design should be taken into consideration; the analysis was performed using embryos rather than patients and a follow-up of children born following the treatments could not be conducted. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - January 5, 2019 Category: Reproduction Medicine Source Type: research

Serum progesterone concentration and live birth rate in frozen–thawed embryo transfers with hormonally prepared endometrium
Publication date: Available online 5 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Isabelle Cédrin-Durnerin, Tiphaine Isnard, Sarah Mahdjoub, Charlotte Sonigo, Alice Seroka, Marjorie Comtet, Charlène Herbemont, Christophe Sifer, Michael GrynbergAbstractResearch questionIs serum progesterone measurement on the day of embryo transfer associated with outcome of frozen–thawed embryo transfer (FET) in cycles using hormonal replacement therapy (HRT) for endometrium preparation?DesignThis single-centre retrospective study assessed the relationship between serum progesterone on embryo transfer d...
Source: Reproductive BioMedicine Online - January 5, 2019 Category: Reproduction Medicine Source Type: research

Bureaucratic overheating is a parasite hampering modern clinical research – A viewpoint from the belly of the beast
Publication date: Available online 2 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Peter Humaidan, Thor HaahrAbstractClinical researchers in Europe experience an increasing amount of bureaucracy which in our eyes has now reached surrealistic heights, hampering the future of research, and thus, the development of new treatment regimens and advances in clinical research. In the long run the current approval and control processes will negatively impact patient care, education, academia, and the health economy of our countries. Thus, at this time a debate is necessary, including not only health care workers, bu...
Source: Reproductive BioMedicine Online - January 3, 2019 Category: Reproduction Medicine Source Type: research

Acupuncture performed around the time of embryo transfer: a systematic review and meta-analysis
Publication date: Available online 2 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Caroline A Smith, Mike Armour, Zewdneh Shewamene, Hsiewe Ying Tan, Robert J Norman, Neil P JohnsonAbstractThis was a systematic review and meta-analysis to examine the efficacy, effectiveness and safety of acupuncture as an adjunct to embryo transfer compared with controls to improve reproductive outcomes. The primary outcome was clinical pregnancy. Twenty trials and 5130 women were included in the review. The meta-analysis found increased pregnancies (risk ratio [RR] 1.32, 95% confidence interval [CI] 1.07–1.62, 12 tri...
Source: Reproductive BioMedicine Online - January 3, 2019 Category: Reproduction Medicine Source Type: research

Soluble LH-hCG receptor and oestradiol as predictors of pregnancy and live birth in IVF
ConclusionssLHCGR levels are negatively related to AMH when oocyte yield is high. High early cycle sLHCGR is associated with elevated day-3 oestradiol. Low sLHCGR and SOR are indicators of increased clinical pregnancy and live birth rates. Patient age and SOR, combined with EOR, might improve prediction of IVF treatment outcomes. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 29, 2018 Category: Reproduction Medicine Source Type: research

Hydrosalpinx and pregnancy loss: a systematic review and meta-analysis
Publication date: Available online 25 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): H.M. Harb, J. Ghosh, F. Al-Rshoud, B. Karunakaran, I.D. Gallos, A. CoomarasamyABSTRACTQuestionWhat is the association of hydrosalpinx with pregnancy loss and the effects of treatment of hydrosalpinx?DesignWe conducted a systematic review and meta-analysis to investigate the risk of pregnancy loss, and the benefit of treatment in women with hydrosalpinx. Searches were conducted on MEDLINE, EMBASE, Cochrane Library and Web of Science.ResultsMeta-analysis of 14 observational studies showed a 74% relative increase in the risk o...
Source: Reproductive BioMedicine Online - December 26, 2018 Category: Reproduction Medicine Source Type: research

Two- and three-dimensional transvaginal ultrasonography for diagnosis of adenomyosis of the inner myometrium
ConclusionsFor the diagnosis of adenomyosis of the inner myometrium, 3D-TVS offers a high accuracy similar to 2D-TVS. However, identification of JZ irregularity with 3D-TVS may be beneficial to diagnosis. Beyond this, a more objective diagnosis may be achieved by using a combination of ≥ two 2D features and ≥ two 3D features. These results may be useful for clinical practice and future research. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 26, 2018 Category: Reproduction Medicine Source Type: research

The effect of serum vitamin D levels in couples on embryo development and clinical outcomes
ConclusionsVitamin D levels, in neither men nor women, was associated with embryo development in cleavage (Day2 and Day3) and blastocyst (Day5/6) stage and clinical outcomes. The possible reason might be that the threshold of vitamin D affecting reproductive process is relatively low, which requires a multicenter study at high latitudes region. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 26, 2018 Category: Reproduction Medicine Source Type: research

Pituitary suppression protocol among Bologna poor responders undergoing ovarian stimulation using corifollitropin alfa: does it play any role?
Publication date: Available online 25 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): J. Errázuriz, P. Drakopoulos, D. Pening, A. Racca, A. Romito, N. De Munck, H. Tournaye, M. De Vos, C. BlockeelABSTRACTResearch questionDoes the type of of pituitary suppression protocol influence cumulative LBR in Bologna poor responders treated with CFA?DesignRetrospective cohort analysis including poor responder patients fulfilling the Bologna criteria who underwent their first ICSI cycle using a CFA-based ovarian stimulation protocol between 2011 and 2017. The starting dose of CFA was 150 μg. Our primary outcom...
Source: Reproductive BioMedicine Online - December 25, 2018 Category: Reproduction Medicine Source Type: research

Individualization of the starting dose of follitropin delta reduces the overall OHSS risk and/or the need of additional preventive interventions: Cumulative data over three stimulation cycles
ConclusionAn individualized follitropin delta dosing regimen decreased the risk of moderate/severe OHSS as well as the incidence of preventive interventions versus a conventional follitropin alfa regimen. An analysis per AMH quartile indicated that these statistically significant differences are driven mainly by patients with the highest pretreatment AMH levels. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Assessment of the calcium releasing machinery in oocytes that failed to fertilize after conventional ICSI and assisted oocyte activation
ConclusionsThe evaluation of spindle-chromosome normalcy and distribution of IP3R1 does not contribute to identify the presence of Ca2+ releasing deficiencies in these oocytes. However, oocyte Ca2+ analysis is of added value to identify Ca2+releasing incapacity of oocytes that failed to fertilize after ICSI or ICSI-AOA. We showed that some patients experiencing fertilization failure after ICSI-AOA present with a suspected activation deficiency downstream of the Ca2+ machinery, which cannot be overcome by ICSI-AOA based on the use of Ca2+ ionophores. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Placental volume and other first-trimester outcomes: are there differences between fresh embryo transfer, frozen-thawed embryo transfer and natural conception?
Publication date: Available online 23 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Cécile Choux, Perrine Ginod, Julie Barberet, Thierry Rousseau, Céline Bruno, Paul Sagot, Karine Astruc, Patricia FauqueABSTRACTResearch questionDoes the mode of conception influence placental volume and other first-trimester outcomes?DesignThis retrospective single-centre case-control study led in Dijon University Hospital included 252 singleton pregnancies (84 in vitro fertilization [IVF] with either fresh embryo transfer [fresh ET] or frozen-thawed embryo transfer [FET] and 168 natural conceptions). First-tr...
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Evaluation of sperm telomere length in infertile men with failed fertilization after intra-cytoplasmic sperm injection
Publication date: Available online 23 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Zahra Darmishonnejad, Marziyeh Tavalaee, Tayebeh Izadi, Somayeh Tanhaei, Mohammad Hossein Nasr-EsfahaniaABSTRACTResearch QuestionTelomeres are non-coding, repetitive DNA sequences (TTAGGG repeats) that play an important role in maintaining genome integrity. Unlike in somatic cells, telomere length in sperm increases with men age and is considered as a molecular marker of sperm quality. Etiology of failed fertilization post- intracytoplasmic sperm injection (ICSI) technique is multifactorial; perhaps one of the reasons for t...
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Inferring biallelism of two FSH receptor mutations associated with spontaneous ovarian hyperstimulation syndrome by evaluating FSH, LH and hCG cross-activity
Publication date: Available online 23 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Clara Lazzaretti, Laura Riccetti, Samantha Sperduti, Claudia Anzivino, Giulia Brigante, Francesco De Pascali, Francesco Potì, Valentina Rovei, Gabriella Restagno, Caterina Mari, Cristina Lussiana, Chiara Benedetto, Alberto Revelli, Livio CasariniAbstractResearch questionWhich is the cumulative effect of two FSHR mutations in sOHSS pathogenesis? Are these mutations in the mono- or biallelic state?DesignTwo follicle stimulating hormone-receptor (FSHR) mutations were found in a pregnant patient affected by spontaneous o...
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Incidence and origin of meiotic whole and segmental chromosomal aneuploidies detected by karyomapping
Publication date: Available online 23 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): David Kubicek, Miroslav Hornak, Jakub Horak, Rostislav Navratil, Gabriela Tauwinklova, Jiri Rubes, Katerina VeselaAbstractResearch questionWhat is the incidence and origin of meiotic whole and segmental aneuploidies detected by karyomapping at a blastocyst stage in human-derived IVF embryos? What is the distribution of various types of errors, including rare chromosomal abnormalities?DesignThe incidence of chromosomal aneuploidies was assessed in 967 trophectoderm biopsies from 180 couples who underwent 215 cycles of IVF wi...
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Endometrial stromal cell proteome mapping in repeated implantation failure and recurrent pregnancy loss cases and fertile women
Publication date: Available online 23 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Lien Dhaenens, Sylvie Lierman, Laura De Clerck, Elisabeth Govaert, Dieter Deforce, Kelly Tilleman, Petra De SutterAbstractResearch questionAre there proteomic differences between endometrial stromal cells of repeated implantation failure (RIF), recurrent pregnancy loss (RPL) and normal fertile women, and is there differential protein expression upon decidualization?DesignThis exploratory study investigated the proteome of in-vitro cultured endometrial stromal cells of women with RIF (n = 4), women with RPL (n = 3) a...
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Ovarian stimulation and endometriosis progression or recurrence: a systematic review
In conclusion, available evidence is generally reassuring (at least for IVF) and does not justify aggressive clinical approaches such as prophylactic surgery before assisted reproductive technology treatment to prevent endometriosis progression or recurrence. However, further evidence is required before being able to reach definitive conclusions. In particular, the potential effects on deep invasive endometriosis and the possible synergistic effect of stimulation and pregnancy are two areas that need to be explored further. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Live birth rates and neonatal outcomes following cleavage-stage versus blastocyst-stage embryo transfer using the freeze-all strategy
ConclusionsThe observed no significant cumulative live birth rate between blastocyst transfer and cleavage transfer could not provide evidence that blastocyst transfer is the preferred strategy. The result indicated singleton births born after blastocyst transfer had a higher risk of preterm delivery compared with cleavage transfer. Further studies are needed to verify the findings. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Novel CFAP43 and CFAP44 mutations cause male infertility with multiple morphological abnormalities of the sperm flagella (MMAF)
ConclusionsThis study demonstrated that CFAP43 and CFAP44 mutations are important causes of MMAF in the Chinese population. These novel mutations further broaden the spectrum of CFAP43 and CFAP44 mutations. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Pro-endometriotic niche in endometriosis
Publication date: Available online 23 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Yanchun Liang, Jinjie Wu, Wei Wang, Hongyu Xie, Shuzhong YaoAbstractEndometriosis is a complex and heterogeneous disorder of unknown etiology. This benign disease possesses special biological behaviors mimicking malignant tumors. The supportive and receptive microenvironment established by existing lesion can be defined as pro-endometriotic nice. After the accumulation of cells by existing lesion, these components display distinct characteristics to impair immune surveillance. As the retrogradation of endometrial stromal ce...
Source: Reproductive BioMedicine Online - December 24, 2018 Category: Reproduction Medicine Source Type: research

Endometrial injury increases Hypoxia-inducible factor expression and angiogenesis in the endometrium of women with recurrent implantation failure
ConclusionsThe results support a series of events that HIF-1α expression, MVD and endometrial apoptosis were reduced in the peri-implantation endometrium of women with RIF which suggest that altered endometrial HIF-1α expression and angiogenesis may contribute to implantation failure. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 23, 2018 Category: Reproduction Medicine Source Type: research

A Comparison of Two Months Pre-Treatment With Gonadotropin-Releasing Hormone Agonists With or Without an Aromatase Inhibitor in Women With Ultrasound Diagnosed Ovarian Endometriomas Undergoing IVF
ConclusionsThe combination of depo-leuprolide acetate monthly for 60 days combined with daily letrozole has better clinical outcomes at IVF in women with endometriomas than depo-leuprolide acetate treatment alone. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 23, 2018 Category: Reproduction Medicine Source Type: research

Biological differences between focal and diffuse adenomyosis and response to hormonal treatment
ConclusionsNo difference was found in the expression of E/P receptors and entity of fibrosis between women with focal and diffuse adenomyosis regardless of GnRHa treatment. A lower expression of E/P receptors in comparison to myoma tissue, potentially clarifies the biological rationale of non-response to hormonal therapies for adenomyosis. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Assessing the use of reflectance confocal microscopy for the non-invasive selection of optimal ovarian cortex fragments for auto transplantation
ConclusionIn this proof of concept study, we have shown that RCM is a promising technique to determine the density of follicles ex vivo in living human ovarian cortex fragments, apparently without compromising the vitality of the tissue. Safety studies and further optimization of the RCM technique with a focus on increasing the penetration depth are required before RCM can be used in a clinical setting. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Impact of human embryonic morphological development on fetal growth parameters: The Rotterdam Periconceptional Cohort (Predict Study)
Publication date: Available online 21 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Francesca Parisi, Melek Rousian, Anton H.J. Koning, Sten P. Willemsen, Eric A.P. Steegers, Régine P.M. Steegers-TheunissenABSTRACTResearch questionIs embryonic morphological development according to the Carnegie stages associated with pregnancy outcome?DesignIn a tertiary hospital-based cohort, 182 singleton non-malformed pregnancies were selected. Serial transvaginal three-dimensional ultrasound (3D US) scans were performed between 6+0 and 10+2 gestational weeks. Embryonic development was annotated according to the ...
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Time-lapse imaging of inner cell mass splitting with Monochorionic Triamniotic Triplets after eSET: A case report
ConclusionsAssisted reproductive techniques and in vitro culture have been associated with an increased frequency of embryo splitting. This has been hypothesised to be linked to the in vitro hatching method observed at the blastocyst stage of development. This case report highlights the need to objectively assess any splitting of the ICM, beyond standard grading of the quality of the ICM and the trophectoderm. Such assessments of ICM splitting should be routine practice in clinical embryology when selecting embryos for transfer. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Vaginal microbiota profile at the time of embryo transfer does not affect live birth rate in IVF cycles with donated oocytes
ConclusionsOur data suggest that BV-like vaginal microbiota at the ET does not directly affect live birth rate. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

5-Methyltetrahydrofolate reduces blood homocysteine level significantly in C677T methyltetrahydrofolate reductase single-nucleotide polymorphism carriers consulting for infertility
ConclusionsCouples with a long history of infertility should be analysed for MTHFR SNP and should be treated with physiological doses of 5-MTHF instead of high doses of folic acid. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Association between Sperm DNA Fragmentation and Idiopathic Recurrent Pregnancy Loss: A Systematic Review & Meta-Analysis
Publication date: Available online 22 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): J Tan, O Taskin, A Albert, MA BedaiwyABSTRACTSperm DNA fragmentation (sDF) has emerged as a valuable tool for male fertility evaluation, yet the relationship between male gamete DNA fragmentation and idiopathic recurrent pregnancy loss remains a topic of ongoing debate. Hence, a meta-analysis was conducted among 12 prospective and 2 retrospective studies involving 530 males with a history of RPL who underwent sperm DNA fragmentation testing compared to 639 fertile controls. Main outcome measures were sDF measured by Comet, ...
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation
ConclusionsDistinctive subpopulations of intestinal microbiota were identified in women with unexplained chronic anovulation. Our results indicate that gut microbiota could be associated with ovarian functions. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Prolonged blastomere movement induced by the delay of pronuclear fading and first cell division adversely affects pregnancy outcomes after fresh embryo transfer on Day 2: a time-lapse study
ConclusionsEmbryos with extended blastomere movement after the first cell division, which is associated with the delay of PN fading and first cell division, have a lower competence to initiate an ongoing pregnancy after fresh embryo transfer on day 2. Thus, blastomere movement could be a useful predictive parameter for selecting embryos at the early cleavage stage. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 22, 2018 Category: Reproduction Medicine Source Type: research

Larger Oocyte Cohorts Maximize Fresh IVF Cycle Birth Rates and Availability of Surplus High-Quality Blastocysts for Cryopreservation
ConclusionsLive birth rates per fresh embryo transfer are highest among cycles with retrieval of 11 or more oocytes. Larger cohorts are not associated with any decline in fresh transfer birth rates. Total potential births per retrieval continue to increase as the number of retrieved oocytes increases. Rates of OHSS remain relatively low after retrieval of large oocyte cohorts if final maturation is triggered with GnRH agonist rather than hCG. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 21, 2018 Category: Reproduction Medicine Source Type: research

Variation Analysis of TMEM150B Gene in Chinese Women with Premature Ovarian Insufficiency
ConclusionThe results suggest that the perturbations in TMEM150B gene are not a common explanation for POI in Chinese women. The role of autophagy played in pathogenic mechanism of POI needs further exploration. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 21, 2018 Category: Reproduction Medicine Source Type: research

Absence of predictable long-term molecular impact of ulipristal acetate (UPA) on the endometrium
ConclusionThe effect of three-month UPA treatment is mostly progesterone receptor (PR) antagonist-like. After treatment discontinuation there are no signs of any long-term effects of this molecule on both endometrial proliferation and maturation. Therefore UPA may be administered to women willing to conceive in the short term without consequences for further implantation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 21, 2018 Category: Reproduction Medicine Source Type: research

Laser technology in the assisted reproductive technology laboratory: a narrative review
Publication date: Available online 21 December 2018Source: Reproductive BioMedicine OnlineAuthor(s): Lien M. Davidson, Yaqiong Liu, Tracey Griffiths, Celine Jones, Kevin CowardABSTRACTTo improve success rates, assisted reproductive technology (ART) procedures continually undergo optimization and enhancement such that we can identify and manipulate the best quality gametes and embryos, and thus improve clinical outcomes. Laser technology is now being applied across ART to reduce procedure times and increase the consistency and reproducibility of traditional ART techniques such as intracytoplasmic sperm injection (ICSI), emb...
Source: Reproductive BioMedicine Online - December 21, 2018 Category: Reproduction Medicine Source Type: research

Live birth rates with a freeze-only strategy versus fresh embryo transfer in subjects with elevated progesterone levels during IVF: secondary analysis of a randomized clinical trial
ConclusionsSerum progesterone level on the day of trigger may have potential as a biomarker on which to base a prospective decision about whether to use a freeze-only or fresh embryo transfer strategy in women undergoing IVF. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 21, 2018 Category: Reproduction Medicine Source Type: research

Analysis of Chromosome Microstructures in Products of Conception Associated with Recurrent Miscarriage
ConclusionsHigh-resolution genome-wide SNP arrays are effective for detecting novel genetic factors causing recurrent miscarriage. A more appropriate reference CNV list may be necessary to more effectively enrich for CNVs likely to cause recurrent miscarriage. Our findings confirmed one non-coding RNA cluster as a strong candidate that may contribute to unexplained miscarriages. Gene expression-regulatory mechanisms may play important roles in the pathogenesis of miscarriages. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - December 21, 2018 Category: Reproduction Medicine Source Type: research