Brachylaima lignieuhadrae n. sp. (Trematoda: Brachylaimidae) from land snails of the genus Euhadra in Japan
In this study, a large-scale snail survey covering a wide area of Japan was conducted to confirm the larval parasite from members of Euhadra and related genera. Sporocysts with cercariae were found only from Eu. brandtii sapporo in Hokkaido and Euhadra callizona in central Honshu at low prevalence (1.0–9.6%). The metacercariae were detected widely from 6 species of Euhadra and the related genera at high prevalence (7.1–100%). A molecular identification by DNA barcoding demonstrated almost all of the larvae to be Brachylaima sp. B. Adult worms experimentally raised from the metacercariae were morphologically mos...
Source: Parasitology International - September 13, 2019 Category: Parasitology Source Type: research

A preliminary report on the awareness and knowledge of seafood-borne parasitic diseases among medical doctors in Australia
Publication date: Available online 12 September 2019Source: Parasitology InternationalAuthor(s): Alexa Seal, Catherine Harding, Shokoofeh ShamsiAbstractDespite the increasing popularity of seafood in Australia and various reports of infection with transmissible parasites in Australian edible aquatic animals such as fish, the number of reported cases of human infections in the country is low. This raised the question that Australian medical doctors may not be fully aware of the presence of these parasites in Australia, which in turn can lead to misdiagnosis of infections. This also may lead to an underestimation of the risk...
Source: Parasitology International - September 13, 2019 Category: Parasitology Source Type: research

A case of high altitude cutaneous leishmaniasis in a non-endemic region in Nepal
Publication date: Available online 11 September 2019Source: Parasitology InternationalAuthor(s): Anup Bastola, Mitesh Shrestha, Mahesh Lamsal, Srijan Shrestha, Sabita Prajapati, Anurag Adhikari, Birendra Prasad Gupta, Mallorie Hide, Lina Devkota, Bimal Sharma Chalise, Kishor Pandey, Krishna Das ManandharAbstractA case of cutaneous leishmaniasis was discovered in a 32-year old man with a persistent erythematous plaque. The patient resides in a high altitude (~2000 m above sea level) area that is not endemic for cutaneous leishmaniasis in the Dunai village of Dolpa, Nepal. The patient's lesion was initially misdiagnosed as...
Source: Parasitology International - September 12, 2019 Category: Parasitology Source Type: research

Genetic diversity of groEL and msp4 sequences of Anaplasma ovis infecting camels from Tunisia
Publication date: Available online 10 September 2019Source: Parasitology InternationalAuthor(s): Rachid Selmi, Mourad Ben Said, Mokhtar Dhibi, Houcine Ben Yahia, Hedi Abdelaali, Lilia MessadiAbstractTo date, no information is available regarding the infection of camels (Camelus dromedarius) by Anaplasma ovis in North African region. Several animal species can be infected by A. ovis which further complicates its natural infection cycle. In this paper, we investigated the occurrence and the genetic diversity of A. ovis in camels and ticks collected from them in Tunisia and the risk factor analysis. Camel blood samples (n =...
Source: Parasitology International - September 12, 2019 Category: Parasitology Source Type: research

Notocotylus primulus n. sp. (Trematoda: Notocotylidae) from the crested duck Lophonetta specularioides (Aves, Anatidae) from Patagonian coast, southwestern Atlantic Ocean
Publication date: Available online 9 September 2019Source: Parasitology InternationalAuthor(s): Julia Inés Diaz, Carmen Gilardoni, Eliana Lorenti, Florencia CremonteAbstractSpecies in the genus Notocotylus (Trematoda, Notocotylidae) are cosmopolitan parasites of mainly aquatic birds and, to a lesser extent, mammals. In this paper we describe Notocotylus primulus n. sp. parasitizing the Crested Duck, Lophonetta specularioides, from the Patagonian coast, southwestern Atlantic Ocean, based on morphological and molecular data. The new species could be distinguished from its congeners by the combination of several featur...
Source: Parasitology International - September 10, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: December 2019Source: Parasitology International, Volume 73Author(s): (Source: Parasitology International)
Source: Parasitology International - September 9, 2019 Category: Parasitology Source Type: research

Association between Cryptosporidium infection and cancer: A systematic review and meta-analysis
Publication date: Available online 28 August 2019Source: Parasitology InternationalAuthor(s): Narges Kalantari, Tahmineh Gorgani-Firouzjaee, Salman Ghaffari, Masomeh Bayani, Taraneh Ghaffari, Mohammad ChehraziAbstractThe link between cryptosporidiosis and cancer has been suggested by some epidemiological studies. This systematic review and meta-analysis was conducted to further understand this relationship. In the current study, six electronic databases were reviewed for Cryptosporidium infection in cancer patients. We used random effects model and 95% confidence intervals (CI) to determine the overall odds ratio (OR). Het...
Source: Parasitology International - August 29, 2019 Category: Parasitology Source Type: research

Integrative taxonomy of Sprentascaris mahnerti and phylogeny of raphidascaridid nematodes (Nematoda: Ascaridoidea: Raphidascarididae) suggest the monophyly of Sprentascaris
Publication date: Available online 27 August 2019Source: Parasitology InternationalAuthor(s): Lennon S. Malta, F. Paiva, Carina Elisei, Luiz E.R. Tavares, Felipe B. PereiraAbstractSprentascaris mahnerti (Nematoda: Raphidascarididae) collected from Loricariichthys labialis (Siluriformes: Loricariidae) in the Pantanal wetlands, State of Mato Grosso do Sul (Brazil), was redescribed using light and scanning electron microscopy (SEM), and genetically characterised along with two other raphidascaridids: Raphidascaroides brasiliensis and Ro. moraveci. Due to the systematic discussion regarding Raphidascaris and Sprentascaris, as ...
Source: Parasitology International - August 28, 2019 Category: Parasitology Source Type: research

Occurrence of dactylogyrid and gyrodactylid Monogenea on common carp, Cyprinus carpio, in the Southern Caspian Basin
In this study, we genetically characterised Monogenea found on the gill of common carp in Iran. In total, 5603 parasites were recovered from 112 fish. The parasites were first identified morphologically as Dactylogyrus extensus, D. anchoratus, D. vastator, D. minutus, D. achmerowi and Gyrodactylus sprostonae. Representative samples were then subjected to sequencing. This is one of the first studies which has provided both morphological and sequence data for Dactylogyrus spp. and G. sprostonae. Our findings provide a foundation for future research into the genetic make-up of these economically significant parasites and the ...
Source: Parasitology International - August 23, 2019 Category: Parasitology Source Type: research

Isolation and characterization of antiprotozoal compound-producing Streptomyces species from Mongolian soils
Publication date: Available online 19 August 2019Source: Parasitology InternationalAuthor(s): Baldorj Pagmadulam, Dugarsuren Tserendulam, Tserennadmid Rentsenkhand, Masayuki Igarashi, Ryuichi Sawa, Coh-ichi Nihei, Yoshifumi NishikawaAbstractNatural resources are recognized as important sources of potential drugs for treating various infections, and microorganisms are a rich natural source of diverse compounds. Among the world's microorganisms, actinomycetes, which are abundant in soil and marine, are the well-known producers of a wide range of bioactive secondary metabolites and antibiotics. In the present study, four acti...
Source: Parasitology International - August 21, 2019 Category: Parasitology Source Type: research

Human dirofilariosis of buccal mucosa – First molecularly confirmed case and literature review
Publication date: Available online 20 August 2019Source: Parasitology InternationalAuthor(s): Stefan Momčilović, Simona Gabrielli, Milan Golubović, Tanja Smilić, Miljan Krstić, Saša Đenić, Marina Ranđelović, Suzana Tasić-OtaševićAbstractWhen presented in unusual manner, human dirofilariosis is hard to recognize and often leads to misdiagnosis and unjustified use of various medications. Herein, we report a rare case of Dirofilaria infection localized in buccal mucosa.A 45-year-old male, residing in the Central Serbia, developed striking edema of buccal mucosa followed by leucocytosis, with both neut...
Source: Parasitology International - August 21, 2019 Category: Parasitology Source Type: research

Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection
This study compared the performance of filter papers used for the preparation of dried blood spots (DBS) in detecting Plasmodium by PCR. Whole blood spiked with P. falciparum-infected erythrocytes to obtain samples with various levels of parasitemia were applied to Whatman 3MM Chr papers, FTA Cards, or FTA Elute Cards to prepare the DBS. DNA was purified from the DBS using a DNA purification kit and used as the template for nested PCR. In probit analysis, the estimated limit of detection (LoD) was 5.5 parasites/μL blood for Whatman 3MM Chr papers and FTA Cards and 1.6 parasites/μL blood for the FTA Elute Card. This r...
Source: Parasitology International - August 21, 2019 Category: Parasitology Source Type: research

Coenzyme Q10 and cerebral malaria in mice: Questionable interpretations, improbable usefulness in humans
Publication date: Available online 14 August 2019Source: Parasitology InternationalAuthor(s): Nyariki James Nyabuga, Alfred Orina Isaac (Source: Parasitology International)
Source: Parasitology International - August 16, 2019 Category: Parasitology Source Type: research

Development of multi-ARMS-qPCR method for detection of hookworms from cats and dogs
In conclusion, the used multi-ARMS-qPCR method has the advantages of high efficiency, sensitivity, specificity, and quantitative analysis and can be used for the clinical detection, epidemiological investigation, and zoonotic risk assessment of canine and feline hookworms. (Source: Parasitology International)
Source: Parasitology International - August 16, 2019 Category: Parasitology Source Type: research

A rebuttal letter to Letter to the Editor by P. Heneberg on “Taxonomic comments on the validity of Echinostoma miyagawai Ishii, 1932 (Trematoda: Echinostomatidae)”
Publication date: Available online 14 August 2019Source: Parasitology InternationalAuthor(s): Uday Kumar Mohanta, Takuya Watanabe, Anisuzzaman, Yuma Ohari, Tadashi Itagaki (Source: Parasitology International)
Source: Parasitology International - August 16, 2019 Category: Parasitology Source Type: research

Coenzyme Q10 and cerebral malaria in mice: Questionable interpretations, improbable usefulness in humans
Publication date: Available online 14 August 2019Source: Parasitology InternationalAuthor(s): Thomas Hanscheid, Martin P. Grobusch (Source: Parasitology International)
Source: Parasitology International - August 16, 2019 Category: Parasitology Source Type: research

Prevalence and molecular characterisation of Calyptospora parasites Overstreet, Hawkins and Fournié, 1984 (Apicomplexa: Calyptosporidae) in fishes from the eastern Amazon, Brazil
Publication date: Available online 14 August 2019Source: Parasitology InternationalAuthor(s): Márcio Charles da Silva Negrão, Maria Regina Lucas da Silva, Marcela Nunes Videira, Lúcio André VianaAbstractThis work reports the occurrence of coccidia of the genus Calyptospora in fishes from the eastern Amazon. Fish were collected on flood plains in the municipality of Macapá, State of Amapá, Brazil. Fresh squash preparations of liver, heart and gallbladder were examined under light microscope. Positive samples of Geophagus proximus and Hoplias malabaricus were used to detect parasites...
Source: Parasitology International - August 16, 2019 Category: Parasitology Source Type: research

Detection and genotyping of Toxoplasma gondii in wild canids in Serbia
Publication date: Available online 9 August 2019Source: Parasitology InternationalAuthor(s): Aleksandra Uzelac, Ivana Klun, Duško Ćirović, Aleksandra Penezić, Vladimir Ćirković, Olgica Djurković-DjakovićAbstractTo gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%...
Source: Parasitology International - August 10, 2019 Category: Parasitology Source Type: research

Eucoleus garfiai (Gállego et Mas-Coma, 1975) (Nematoda: Capillariidae) infection in wild boars (Sus scrofa leucomystax) from the Amakusa Islands, Japan
We examined lingual tissues of Japanese wild boars (Sus scrofa leucomystax) captured in the Amakusa Islands off the coast of Kumamoto Prefecture. One hundred and forty wild boars were caught in 11 different locations in Kamishima (n = 36) and Shimoshima (n = 104) in the Amakusa Islands, Japan between January 2016 and April 2018. Lingual tissues were subjected to histological examinations, where helminths and their eggs were observed in the epithelium of 51 samples (36.4%). No significant differences in prevalence were observed according to maturity, sex or capture location. Lingual tissues positive for helminth inf...
Source: Parasitology International - August 9, 2019 Category: Parasitology Source Type: research

In vitro effects of the asymmetric peptidomimetic 157, containing l-tartaric acid core and valine/leucine substituents, on Leishmania amazonensis promastigotes and amastigotes
Publication date: Available online 6 August 2019Source: Parasitology InternationalAuthor(s): André L.S. Santos, Filipe P. Matteoli, Diego S. Gonçalves, Sergio H. Seabra, Maria Teresa V. Romanos, Marta H. Branquinha, Gabriel O. Resende, Bruno A. Cotrim, Lucia C.S. Aguiar, Leandro S. SangenitoAbstractThe current treatments for leishmaniasis bump into several obstacles, including low efficacy, high costs, long monitoring, and several/severe side effects. Consequently, the search for promising compounds is a tangible need. Recently, we reported the anti-Leishmania amazonensis action of asymmetric peptidomimetic c...
Source: Parasitology International - August 7, 2019 Category: Parasitology Source Type: research

Morphological and molecular characterization of Apatemon sp. infecting killifish in Mozambique
In this study, we report morphometrical and molecular characterization of Apatemon sp. metacercariae parasitizing killifish Nothobranchius furzeri (Cyprinodontiformes: Nothobranchiidae) in south-east Mozambique. Metacercariae obtained from the cerebral cavity of killifish and two adult individuals isolated from experimentally infected ducklings were used for detailed morphological and molecular description, both resulting in generic affiliation to Apatemon. This is the first molecularly confirmed record of this trematode genus in Africa. Considering the morphological variability and wide host range of individual Apatemon s...
Source: Parasitology International - July 28, 2019 Category: Parasitology Source Type: research

A chloroquinoline derivate presents effective in vitro and in vivo antileishmanial activity against Leishmania species that cause tegumentary and visceral leishmaniasis
Publication date: Available online 27 July 2019Source: Parasitology InternationalAuthor(s): Jessica K.T. Sousa, Luciana M.R. Antinarelli, Débora V.C. Mendonça, Daniela P. Lage, Grasiele S.V. Tavares, Daniel S. Dias, Patrícia A.F. Ribeiro, Fernanda Ludolf, Vinicio T.S. Coelho, João A. Oliveira-da-Silva, Luísa Perin, Bianka A. Oliveira, Denis F. Alvarenga, Miguel A. Chávez-Fumagalli, Geraldo C. Brandão, Vandack Nobre, Guilherme R. Pereira, Elaine S. Coimbra, Eduardo A.F. CoelhoAbstractThe identification of new therapeutics to treat leishmaniasis is desirable, since available d...
Source: Parasitology International - July 28, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: October 2019Source: Parasitology International, Volume 72Author(s): (Source: Parasitology International)
Source: Parasitology International - July 18, 2019 Category: Parasitology Source Type: research

Three-dimensional fine structure of feeder organelle in Cryptosporidium parvum
Publication date: Available online 9 July 2019Source: Parasitology InternationalAuthor(s): Hiroki Bochimoto, Daisuke Kondoh, Yo Ishihara, Mohammad Hazzaz Bin Kabir, Kentaro KatoAbstractFeeder organelles of Cryptosporidium are the convoluted structures located at the host-parasite interface that uptake of nutrients from host cells. Although the ultrastructure of feeder organelles has been summarized as being highly invaginated structure, the three-dimensional form remains uncertain. Osmium-maceration scanning electron microscopy (OS-SEM) allows visualization of the three-dimensional ultrastructure after removing soluble pro...
Source: Parasitology International - July 11, 2019 Category: Parasitology Source Type: research

Microsatellite analysis reveals extensive gene flow, and lack of population structure in the farm populations of Haemonchus contortus in northern China
Publication date: Available online 9 July 2019Source: Parasitology InternationalAuthor(s): Sawar Khan, Xiaoping Luo, Chunxiu Yuan, Xiaochao Zhao, Ayesha Nisar, Junyan Li, Xiangshu Yang, Jiayan Zhang, Xingang FengAbstractThe parasitic nematode Haemonchus contortus is economically an important parasite of small ruminants across the globe. China is the world's largest producer, consumer, and importer of mutton. With ubiquitous distribution across the country H. contortus is one of the potential candidates to cause huge economic losses to small ruminant farming industry in China. We herein investigated genetic diversity and po...
Source: Parasitology International - July 11, 2019 Category: Parasitology Source Type: research

Toxocara related peritonitis: A case report and review of literature
Publication date: Available online 4 July 2019Source: Parasitology InternationalAuthor(s): Ferhat Arslan, Naciye Betul Baysal, Ahmet Aslan, Bengu Cobanoglu Ates, Haluk VahabogluAbstractToxocariasis is a clinical syndrome caused by the larvae of two ascarid nematodes, namely, Toxocara canis and T. cati that live in dogs and cats as definitive hosts. Humans acquire Toxocara infection by accidental consumption of eggs contaminated foods, soil, water or larvae encapsulated in the viscera or meats of various paratenic hosts e.g., chicken. After oral ingestion, the ova hatch and the free larvae penetrate the intestinal wall to m...
Source: Parasitology International - July 5, 2019 Category: Parasitology Source Type: research

Practical approaches to interpretation of findings from a systematic review and network meta-analysis on efficacy and resistance of different artemisinin-based combination therapies
Publication date: Available online 29 June 2019Source: Parasitology InternationalAuthor(s): Rama Jayaraj, Chellan Kumarasamy, Madurantakam Royam Madhav (Source: Parasitology International)
Source: Parasitology International - June 30, 2019 Category: Parasitology Source Type: research

Curcumin pre-treatment modulate the activities of adenine nucleotide and nucleoside degradation enzymes in lymphocyte of rats infected with Trypanosoma evansi
This study aimed to evaluate nucleoside triphosphate diphosphohydrolase (NTPDase) and adenosine deaminase (ADA) activities in lymphocytes from rats supplemented or not with curcumin 30 days prior to experimental infection with Trypanosoma evansi. Thirty-two adult male Wistar rats were divided in four groups. The pre-infection group 20 (PreI20) received orally 20 mg/kg of curcumin and pre-infection group 60 (PreI60) received orally 60 mg/kg of curcumin for 30 days prior inoculation with T. evansi. The infected e non-infected control groups received only oral vehicle for 30 days. Trypanosoma evansi infected group...
Source: Parasitology International - June 26, 2019 Category: Parasitology Source Type: research

Two new species of ascaridoid nematodes in Brazilian Crocodylomorpha from the upper cretaceous
Publication date: Available online 21 June 2019Source: Parasitology InternationalAuthor(s): Daniel F.F. Cardia, Reinaldo J. Bertini, Lucilene G. Camossi, Luiz A. LetizioAbstractTwo new ascaridoid species, Bauruascaris cretacicus n. gen. et n. sp., and Bauruascaris adamantinensis n. gen. et n. sp., are described based on the fossils of eggs preserved in 80–70 million year old phosphatized coprolites of Crocodyliformes, chronologically assigned to the Upper Cretaceous (Campanian/Maastrichtian age), collected from sedimentary rocks of the Bauru Group, Adamantina Formation in the municipality of Santo Anastácio, i...
Source: Parasitology International - June 23, 2019 Category: Parasitology Source Type: research

Unconventional support for a raptorial niche division between Australaves and Afroaves: The distribution of helminths
In conclusion, we provide the first systematically collected evidence on the congruence of the helminth distribution and phylogeny of the raptorial niche, which is consistent with its split into Australaves and Afroaves.Graphical abstract (Source: Parasitology International)
Source: Parasitology International - June 21, 2019 Category: Parasitology Source Type: research

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe
This study reveals the presence of H. felis in Austria and provides more evidence on the geographical distribution and pathogenicity of this parasite for domestic cats. To the authors' knowledge, this is the first autochthonous case of feline hepatozoonosis in Central Europe.Graphical abstract (Source: Parasitology International)
Source: Parasitology International - June 20, 2019 Category: Parasitology Source Type: research

Human case of Onchocerca dewittei japonica infection in Fukushima, Northeastern Honshu, Japan
Publication date: Available online 17 June 2019Source: Parasitology InternationalAuthor(s): Masako Fukuda, Shigehiko Uni, Takahiro Igari, Yasufumi Utsumi, Yasushi Otsuka, Jun Nakatani, Shoji Uga, Tatsuru Hara, Hideo Hasegawa, Hiroyuki TakaokaAbstractA 73-year-old man living in Kawamata-machi, Fukushima Prefecture, Northeastern Honshu, Japan, visited a hospital with complaints of a subcutaneous swelling that had developed on the back of his left hand. The nodule was surgically removed from the vagina fibrosa tendinis of his left forefinger. Based on the histopathological characteristics, the causative agent of this nodule w...
Source: Parasitology International - June 18, 2019 Category: Parasitology Source Type: research

Littermate cats rescued from a shelter succumbed to acute, primary toxoplasmosis associated with TOXO DB genotype #4, generally circulating in wildlife
Publication date: Available online 17 June 2019Source: Parasitology InternationalAuthor(s): E.E.V. Crouch, L.D. Mittel, T.L. Southard, C.K. Cerqueira-Cézar, F.H.A. Murata, O.C.H. Kwok, C. Su, J.P. DubeyAbstractCats are important in the epidemiology of Toxoplasma gondii infection because they are the only hosts that can excrete the environmentally resistant oocysts in the environment. Although exposure is common (approximately 30% of cats in the USA), clinical toxoplasmosis is relatively rare. Here, we report overwhelming disseminated toxoplasmosis in two litter mate 8-week-old kittens, thought to have acquired toxop...
Source: Parasitology International - June 18, 2019 Category: Parasitology Source Type: research

An ecological study of eosinophilic meningitis caused by the nematode, Angiostrongylus cantonensis (Chen, 1935) (Nematoda: Metastrongylidae)
In conclusion, wind speed is significantly negatively correlated with the number of EOM patients. (Source: Parasitology International)
Source: Parasitology International - June 18, 2019 Category: Parasitology Source Type: research

The curious case of the endemic freshwater crocodile Crocodylus mindorensis as incidental host of marine fish acanthocephalan
Publication date: Available online 12 June 2019Source: Parasitology InternationalAuthor(s): Arvin Jet B. Marcaida, Misako Urabe, Jonathan Carlo A. Briones, Mae Lowe L. Diesmos, Marisa Tellez, Arvin C. DiesmosAbstractWe performed the first host-parasite survey of the Philippine crocodile, Crocodylus mindorensis, a critically endangered species for which ecological information is lacking. We collected by gastric lavage samples of the stomach contents of crocodiles (n = 10) residing at the Palawan Wildlife Rescue and Conservation Center in Puerto Princesa, Palawan, Philippines. The only parasite detected was an acanthocep...
Source: Parasitology International - June 12, 2019 Category: Parasitology Source Type: research

Sero-diagnostic potential of Plasmodium falciparum recombinant merozoite surface protein (MSP)-3 expressed in silkworm
Publication date: Available online 12 June 2019Source: Parasitology InternationalAuthor(s): Vipin Kumar Deo, Yu Inagaki, Elsa Herdiana Murhandarwati, Widya Asmara, Takatsugu Miyazaki, Tatsuya Kato, Enoch Y. ParkAbstractPlasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. Strategies are directed to combat malaria including development of diagnostic tools, serological markers and vaccinations. A target under intensive studies is Merozoite Surface Protein (MSP)-3. The aim of this study is to express and pur...
Source: Parasitology International - June 12, 2019 Category: Parasitology Source Type: research

Description of a new species, Cryptocotyle lata sp. nov., and discussion of the phylogenetic relationships in Opisthorchioidea
Publication date: Available online 12 June 2019Source: Parasitology InternationalAuthor(s): Yulia V. Tatonova, Vladimir V. BesprozvannykhAbstractAdult Cryptocotyle lata sp. nov. worms were obtained from experimental studies. In the Russian southern Far East, the life cycle of this parasite is carried out using freshwater snails (Boreoelona ussuriensis), freshwater fish, and birds as the first intermediate, second intermediate, and definitive hosts, respectively. The morphological indices of C. lata sp. nov. are closest to Cryptocotyle concava; however, these two species differ in terms of their sizes of body, oral and vent...
Source: Parasitology International - June 12, 2019 Category: Parasitology Source Type: research

First molecular identification of Strongyloides vituli in cattle in Japan and insights into the evolutionary history of Strongyloides parasites of ruminants
Publication date: Available online 31 May 2019Source: Parasitology InternationalAuthor(s): Phoo Pwint Ko, Kohei Sakaguchi, Ayako Yoshida, Haruhiko Maruyama, Nariaki Nonaka, Eiji NagayasuAbstractTraditionally, Strongyloides nematode infecting cattle had been thought to be a single species, S. papillosus. Surprisingly, Eberhardt et al. in 2008 reported two, rather than one Strongyloides species infected cattle, with one being S. papillosus and the other S. vituli. However, there was no subsequent report to support their findings.In July 2018, a case of a sudden death of a calf believed to be due to heavy infection with S. pa...
Source: Parasitology International - June 2, 2019 Category: Parasitology Source Type: research

Distribution records of three species of Leucochloridium (Trematoda: Leucochloridiidae) in Japan, with comments on their microtaxonomy and ecology
In this study, old scientific records, sighting information on broodsacs from the general public, and direct molecular evidence by DNA barcoding clearly showed that at least three species of Leucochloridium are distributed in Japan. A vertical-striped broodsac found from Succinea sp. in Okinawa, the subtropical island of Japan, were treated as Leucochloridium sp., but being almost identical to that of Leucochloridium passeri in neighboring Taiwan. The European species of Leucochloridium perturbatum and Leucochloridium paradoxum were frequently detected from Succinea lauta in Hokkaido, the northernmost island of Japan. The ...
Source: Parasitology International - May 31, 2019 Category: Parasitology Source Type: research

A Toxoplasma gondii strain isolated in Okinawa, Japan shows high virulence in Microminipigs
In this study, we examined the virulence using the Microminipig. After infection, four out of five infected pigs showed severe clinical symptoms: inappetence, hypoactivity and tachypnea. Eventually, three out of the five infected pigs succumbed before the end of the observation. Among the three dead pigs, histological analysis revealed that interstitial pneumonia and spotty necrosis in the liver indicating that the TgCatJpOk4 strain has a high virulence not only in laboratory mice, but in pigs as well.Graphical abstract (Source: Parasitology International)
Source: Parasitology International - May 31, 2019 Category: Parasitology Source Type: research

A comparison of body condition of the yellowstriped butterfish Labracoglossa argenteiventris in relation to parasitism by the cymothoid isopod Ceratothoa arimae
In this study, to test this hypothesis on another Ceratothoa–fish relationship in different region, we examined the effects of Ceratothoa arimae on the body condition of the yellowstriped butterfish, Labracoglossa argenteiventris, a commercial fish important to local fisheries particularly in the remote islands of Tokyo, Japan. Ceratothoa arimae was found in 8 out of 23 fish examined (prevalence: 34.8%). Although infected and uninfected fish were similar in standard length, the body condition index (relative weight) of infected fish was significantly lower than that of uninfected fish. The observed weight of infected...
Source: Parasitology International - May 24, 2019 Category: Parasitology Source Type: research

Treatment of resistant visceral leishmaniasis with interferon gamma in combination with liposomal amphotericin B and allopurinol
This report describes the first case of visceral leishmaniasis (VL) resistant to pentavalent antimonials and also the first use of combinational therapy in Iran. The patient was a two-year old boy, from a non-endemic area for leishmaniasis in northern Iran, presenting with pentavalent antimonial resistant VL. Additional treatment with conventional and liposomal amphotericin B was not effective. A complete cure was achieved following a three week treatment with liposomal amphotericin B (5 mg/kg/day for 5 days, then on the 14th and 21st days), allopurinol (25 mg/day for 5 days, then on the 14th and 21st days) and int...
Source: Parasitology International - May 24, 2019 Category: Parasitology Source Type: research

Highly specific and sensitive anti-Strongyloides venezuelensis IgY antibodies applied to the human strongyloidiasis immunodiagnosis
This study aimed to produce and fractionate IgY antibodies from the egg yolks of hens that were immunized with the total antigenic extracts of Strongyloides venezuelensis infectious filariform larvae (iL3) and parthenogenetic females (pF). IgY antibodies were then evaluated by their recognition of antigenic proteins, evolutive helminth forms, and serological diagnosis of human strongyloidiasis by the detection of immune complexes in serum samples. Egg yolks were fractionated to obtain IgY antibodies by thiophilic interaction chromatography. Immune complex detection in serum samples showed diagnostic values for anti-iL3 IgY...
Source: Parasitology International - May 24, 2019 Category: Parasitology Source Type: research

Molecular analysis reveals expansion of Fasciola hepatica distribution from Afghanistan to China
Publication date: Available online 21 May 2019Source: Parasitology InternationalAuthor(s): Tran Nhat Thang, Hakimullah Hakim, Raihana Royan Rahimi, Madoka Ichikawa-SekiAbstractRecently, phosphoenol pyruvate carboxykinase (pepck) and DNA polymerase delta (pold) were established as reliable nuclear markers for species identification of Fasciola spp. in multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism-based assays, respectively. Currently, little is known about Fasciola species distribution in Central Asia. Therefore, the objective of the present study was to perform precise molecular...
Source: Parasitology International - May 22, 2019 Category: Parasitology Source Type: research

Leishmania donovani adenylate kinase 2a prevents ATP-mediated cell cytolysis in macrophages
Publication date: Available online 17 May 2019Source: Parasitology InternationalAuthor(s): P.G. Kulkarni, N. Shah, B.N. Waghela, C.M. Pathak, A. PappachanAbstractIn Leishmania spp. ATP utilizing enzymes serves as a key role in preserving integrity of host cells for survival of parasite. Earlier reports suggested that Adenylate kinase (AK) a phosphotransferase enzyme released by Leishmania donovani secretome, involved in modulating levels of NTPs. In the present study, we cloned, expressed and characterized recombinant putative AK. Based on a sequence and phylogeny analysis, we identified the prominent features of the seven...
Source: Parasitology International - May 18, 2019 Category: Parasitology Source Type: research

Evaluation of antileishmanial efficacy of Salidroside against the SSG-sensitive and resistant strain of Leishmania donovani
Publication date: Available online 17 May 2019Source: Parasitology InternationalAuthor(s): Kalpana Chauhan, Gurpreet Kaur, Sukhbir KaurAbstractThe successful control and eradication of leishmaniasis are still challenging in view of the lack of adequate chemotherapy and potential prophylaxis. Research is going on for finding an appropriate anti-leishmanial drug which should be acceptable in terms of cost and safety. In view of this, the current study investigated the anti-leishmanial efficacy of salidroside (SAL) which is a phenylpropanoid glycoside. The leishmanicidal capacity of SAL was verified in vitro as well as in viv...
Source: Parasitology International - May 18, 2019 Category: Parasitology Source Type: research

Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia
Publication date: Available online 17 May 2019Source: Parasitology InternationalAuthor(s): Fitrine Ekawasti, Wisnu Nurcahyo, April Hari Wardhana, Tomoyuki Shibahara, Masaharu Tokoro, Kazumi Sasai, Makoto MatsubayashiAbstractGastrointestinal parasites including Eimeria spp. are known to affect domestic animal productivity causing watery or lethal bloody diarrhea. However, there are few reports on the detailed distribution of bovine Eimeria spp. in cattle, particularly in developing tropical and sub-tropical areas. Using a total of 289 fecal samples collected from beef cattle on Java Island, one of the five main islands of I...
Source: Parasitology International - May 17, 2019 Category: Parasitology Source Type: research

Circulating endothelial cells in severe Plasmodium falciparum infection
This study aimed to measure blood CECs by flow cytometery in patients with acute malaria infection before and after treatment and to evaluate the prognostic value of that measurement for that disease. The subjects were allocated into: Group I: uncomplicated malaria (UM, n = 32), Group II: severe malaria (SM, n = 12), Group III: the treated UM (TUM, n = 32), Group IV: the treated SM (TSM, n = 12) and Group V: healthy controls (HC, n = 25). Before treatment, SM patients showed the highest mean number of CECs (30,658.3 ± 2658.2/5 × 106 peripheral blood mononuclear cells (PBMCs)), follo...
Source: Parasitology International - May 15, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: August 2019Source: Parasitology International, Volume 71Author(s): (Source: Parasitology International)
Source: Parasitology International - May 13, 2019 Category: Parasitology Source Type: research

Molecular investigation of tick-borne infections in cattle from Xinjiang Uygur Autonomous Region, China
In this study, nested polymerase chain reaction (PCR) assays and gene sequencing were used to detect and analyze epidemiological features of Babesia bovis, B. bigemina, Coxiella burnetii and Anaplasma bovis infections in XUAR. Out of 195 samples tested, 24 (12.3%), 67 (34.4%), 40 (20.5%) and 10 (5.1%) were positive for B. bovis, B. bigemina, C. burnetii and A. bovis, respectively. Sequencing analysis indicated that B. bovis SBP-4, B. bigemina Rap1a, C. burnetii htpB and A. bovis 16S rRNA genes from XUAR showed 99%–100% identity with documented isolates from other countries. Phylogenetic analyses revealed that B. bovi...
Source: Parasitology International - May 9, 2019 Category: Parasitology Source Type: research