Nose-to-brain co-delivery of repurposed simvastatin and BDNF synergistically attenuates LPS-induced Neuroinflammation
Publication date: Available online 23 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Dharani Manickavasagam, Li Lin, Moses O. OyewumiAbstractA therapeutic strategy that can combat the multifaceted nature of neuroinflammation pathology was investigated. Thus, we fabricated PEG-PdLLA polymersomes and evaluated the efficacy in co-delivery of simvastatin (Sim, as a repurposed anti-inflammatory agent) with brain derived neurotrophic factor (BDNF, as an exogeneous trophic factor supplementation). Using LPS model of neuroinflammation, intranasal administration of combination drug-loaded polymerso...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 24, 2019 Category: Nanotechnology Source Type: research

Purinergic receptor (P2X7) activation reduces cell–cell adhesion between tubular epithelial cells of the proximal kidney
Publication date: Available online 23 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Eleftherios Siamantouras, Gareth W. Price, Joe A. Potter, Claire E. Hills, Paul E. SquiresAbstractLoss of epithelial (E)-cadherin mediated cell–cell adhesion impairs gap junction formation and facilitates hemichannel-mediated ATP release in the diabetic kidney. Linked to inflammation and fibrosis, we hypothesized that local increases in inter-cellular ATP activate P2X7 receptors on neighboring epithelial cells of the proximal tubule, to further impair cell–cell adhesion and ultimately exacerbat...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 24, 2019 Category: Nanotechnology Source Type: research

Label-free liquid biopsy based on blood circulating DNA detection using SERS-based nanotechnology for nasopharyngeal cancer screening
Publication date: Available online 21 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Duo Lin, Qiong Wu, Sufang Qiu, Guannan Chen, Shangyuan Feng, Rong Chen, Haishan ZengAbstractDevelopment of a sensitive, rapid and easy-to-use liquid biopsy method is of imperative clinical value for point-of-care caner diagnostics. Here, a label-free and modification-free nanotechnology based on surface-enhanced Raman spectroscopy (SERS) was employed for DNA analysis. Using the SERS signals of phosphate backbone as internal standard, quantitative detection for nucleobases was achieved even at single base l...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 22, 2019 Category: Nanotechnology Source Type: research

Raman profiling of circulating extracellular vesicles for the stratification of Parkinson’s patients
Publication date: Available online 21 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Alice Gualerzi, Silvia Picciolini, Cristiano Carlomagno, Federica Terenzi, Silvia Ramat, Sandro Sorbi, Marzia BedoniAbstractParkinson’s disease (PD) is a chronic neurodegenerative disorder, characterized by considerable clinical heterogeneity. Extracellular vesicles (EVs) were proposed as new biomarkers for PD because of their role as vehicles of multiple PD related molecules, but technical limitations exist in their detection and characterization in a clinical environment. We propose herein a Raman ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 22, 2019 Category: Nanotechnology Source Type: research

A multilamellar nanoliposome stabilized by interlayer hydrogen bonds increases antimalarial drug efficacy
Publication date: Available online 21 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Wesley L. Fotoran, Thomas Müntefering, Nicole Kleiber, Beatriz N.M. Miranda, Eva Liebau, Darrell J. Irvine, Gerhard WunderlichAbstractLipid particles for drug delivery can be modified to create multilayer vesicles with higher stability and improved cargo interaction. Here, we used lipids capable of forming hydrogen bonds instead of covalent bonds and designed stable vesicles-inside-vesicles with a high capacity of entrapping antimalarial drugs such as chloroquine (hydrophilic) and Artemisinin (lipophi...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 22, 2019 Category: Nanotechnology Source Type: research

Iguratimod encapsulated PLGA-NPs improves therapeutic outcome in Glioma, Glioma stem-like cells and Temozolomide resistant Glioma cells
Publication date: Available online 22 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Muhammad Younis, Wang Faming, Zhao Hongyan, Tan Mengmeng, Song Hang, Yuan LiudiAbstractGlioma is the most common neoplasm of the central nervous system, with the highest mortality rate. The present study was designed to examine the therapeutic effect of Iguratimod (IGU) encapsulated- poly (lactic-co-glycolic acid) PLGA nanoparticles (IGU-PLGA-NPs), which showed inhibition of glioma cells proliferation both in vitro and in vivo. IGU was encapsulated in PLGA nanoparticles with an average size of 100-200 nm w...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 22, 2019 Category: Nanotechnology Source Type: research

Site-specific intestinal DMT1 silencing to mitigate iron absorption using pH-sensitive multi-compartmental nanoparticulate oral delivery system
We report a novel pH-sensitive multi-compartmental nanoparticulate (MCP) oral delivery system that encapsulates siRNA for silencing the divalent metal transporter 1 (DMT1) gene, a primary iron transporter highly expressed in the intestine. The gelatin nanoparticles-coated with Eudragit® L100–55 are able to protect siRNA from intracellular degradation and allow for sustained release of siRNA in the cytosol. When orally-administered, Eudragit® L100–55 based MCPs containing DMT1 siRNA/gelatin NPs specifically and significantly decreased the expression of DMT1 mRNA in the duodenum, which consequently decrea...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 16, 2019 Category: Nanotechnology Source Type: research

Targeted co-delivery of Trp-2 polypeptide and monophosphoryl lipid A by pH-sensitive poly (β-amino ester) nano-vaccines for melanoma
Publication date: Available online 5 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Chenming Zou, Guiying Jiang, Xueqin Gao, Wei Zhang, Huan Deng, Chong Zhang, Jiahui Ding, Rui Wei, Xueqian Wang, Ling Xi, Songwei TanAbstractDendritic cell (DC)-targeted vaccines based on nanotechnology are a promising strategy to efficiently induce potent immune responses. We synthesized and manufactured a mannose-modified poly (β-amino ester) (PBAE) nano-vaccines with easily tuneable and pH-sensitive characteristics to co-deliver the tumour-associated antigen polypeptide Trp-2 and the TLR4 agonist mon...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 6, 2019 Category: Nanotechnology Source Type: research

Hydrogel-embedded gold nanorods activated by plasmonic photothermy with potent antimicrobial activity
Publication date: Available online 13 September 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Carlos Bermúdez-Jiménez, Marc G. Romney, Samuel A. Roa-Flores, Gabriel Martínez-Castañón, Horacio BachAbstractPlasmonic photothermal therapy (PPTT) has been used as an alternative to chemotherapy for the elimination of resistant microorganisms; however, its in situ evaluation has not been well studied. In the present study, we assessed the antimicrobial activity of a chitosan-based hydrogel embedded with gold nanorods (Ch/AuNRs) using a low power infrared diode laser. T...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - September 14, 2019 Category: Nanotechnology Source Type: research

Bioactive proteins delivery through core-shell nanofibers for meniscal tissue regeneration
Publication date: Available online 4 September 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Jihye Baek, Emily Lee, Martin K Lotz, Darryl D D'LimaAbstractMimicking the ultrastructural morphology of the meniscus with nanofiber scaffolds, coupled with controlled growth-factor delivery to the appropriate cells, can help engineer tissue with the potential to grow, mature, and regenerate after in vivo implantation. We electrospun nanofibers encapsulating platelet-derived growth factor (PDGF-BB), which is a potent mitogen and chemoattractant in a core of serum albumin contained within a shell of polyla...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - September 5, 2019 Category: Nanotechnology Source Type: research

Tumor-specific delivery of a paclitaxel-loading HSA-haemin nanoparticle for cancer treatment
Publication date: Available online 2 September 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Hea-Jong Chung, Hyeon-Jin Kim, Seong-Tshool HongAbstractA cancer-targeted chemotherapy could potentially eradicate cancers if anticancer drugs are delivered precisely to the cancers. Although various types of nanoparticles have been developed for cancer-specific delivery of anticancer drugs, the drug delivery capabilities of these nanoparticles were not specific enough to eradicate cancer. Here, we developed a targeting-enhancing nanoparticle of paclitaxel, in which paclitaxel was encapsulated with a huma...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - September 3, 2019 Category: Nanotechnology Source Type: research

Recuperative effect of metformin loaded Polydopamine Nanoformulation promoting EZH2 mediated proteasomal degradation of phospho-α-Synuclein in Parkinson's disease model
Publication date: Available online 31 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mo. Nadim Sardoiwala, Anup K. Srivastava, Babita Kaundal, Surajit Karmakar, Subhasree Roy ChoudhuryAbstractPosttranslational modification and agglomeration of α-Synuclein (α-Syn), mitochondrial dysfunction, oxidative stress and loss of dopaminergic neurons are hallmark of Parkinson's disease (PD). This paper evaluates neuroprotection efficacy of nature inspired bio-compatible polydopamine nanocarrier for metformin delivery (Met encapsulated PDANPs) by crossing blood brain barrier in in vitro, 3D...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 31, 2019 Category: Nanotechnology Source Type: research

Mitochondria-targeted delocalized lipophilic cation complexed with human serum albumin for tumor cell imaging and treatment
In this study, human serum albumin (HSA) complexed with 5BMF (5BMF@HSA) has been developed to further improve its solubility (from 1.61 to 5.41 mg/mL), and the fluorescent intensity of 5BMF@HSA was improved over 2 times. Nearly 10-fold 5BMF was released from 5BMF@HSA complex in acidic condition when compared with neutral/basic environment. Intracellular distribution of 5BMF was altered by HSA as its signals were observed in lysosomes where free 5BMF barely localized. Both 5BMF@HSA and 5BMF showed selective toxicity toward tumor cells in μM and nM range and effectively inhibited tumor growth in mice model. In summar...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 25, 2019 Category: Nanotechnology Source Type: research

RGD-functionalized magnetosomes are efficient tumor radioenhancers for X-rays and protons
Publication date: Available online 24 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Maha Hafsi, Sandra Preveral, Christopher Hoog, Joel Hérault, Géraldine Adryanczyk Perrier, Christopher T Lefèvre, Hervé Michel, David Pignol, Jérôme Doyen, Thierry Pourcher, Olivier Humbert, Juliette Thariat, Béatrice CambienAbstractAlthough chemically synthesized ferro/ferrimagnetic nanoparticles have attracted great attention in cancer theranostics, they lack radio-enhancement efficacy due to low targeting and internalization ability. Herein, we investigated...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 25, 2019 Category: Nanotechnology Source Type: research

pH-triggered delivery of magnetic nanoparticles depends on tumor volume
Publication date: Available online 23 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Alexandra G. Pershina, Olga Ya. Brikunova, Alexander M. Demin, Oleg B. Shevelev, Ivan A. Razumov, Evgenii L. Zavjalov, Dina Malkeyeva, Elena Kiseleva, Nadezhda V. Krakhmal’, Sergey V. Vtorushin, Vasily L. Yarnykh, Vladimir V. Ivanov, Raisa I. Pleshko, Victor P. Krasnov, Ludmila M. OgorodovaAbstractNowadays there is growing recognition of the fact that biological systems have a greater impact on nanoparticle target delivery in tumors than nanoparticle design. Here we investigate the targeted delivery o...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 24, 2019 Category: Nanotechnology Source Type: research

A pH-sensitive coordination polymer network-based nanoplatform for magnetic resonance imaging-guided cancer chemo-photothermal synergistic therapy
This study demonstrated that the Fe-GA network-based nanoparticle is a promising diagnostic and therapeutic agent for theranostic application and further clinic translation.Graphical AbstractIllustration of a multifunctional iron-gallic acid (Fe-GA) network-based nanoparticle as a theranostic agent for magnetic resonance imaging-guided photothermal therapy and chemotherapy. The results suggest that Fe-GA network-based nanoplatform is safe and practical, and can effectively kill tumor cells under chemo-photothermal synergistic therapy. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 21, 2019 Category: Nanotechnology Source Type: research

Reduction-responsive polypeptide nanomedicines significantly inhibit progression of orthotopic osteosarcoma
In this study, the reduction-responsive polypeptide micelles based on methoxy poly(ethylene glycol)-block-poly(S-tert-butylmercapto-L-cysteine) copolymers (mPEG113-b-PBMLC4, P4M, and mPEG113-b-PBMLC9, P9M) were developed to control the delivery of doxorubicin (DOX) in OS therapy. Compared to free DOX, P4M/DOX and P9M/DOX exhibited 2.6 and 3.5 times increases in the area under the curve (AUC) of pharmacokinetics, 1.6 and 2.0 times increases in tumor accumulation, and 1.6 and 1.7 times decreases the distribution in the heart. Moreover, the selective accumulation of micelles, especially P9M/DOX, in tumors induced stronger ant...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 21, 2019 Category: Nanotechnology Source Type: research

Introducing article numbering to Nanomedicine: Nanotechnology, Biology, and Medicine
Publication date: August 2019Source: Nanomedicine: Nanotechnology, Biology and Medicine, Volume 20Author(s): (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 18, 2019 Category: Nanotechnology Source Type: research

Gold nanourchins induce cellular stress, impair proteostasis and damage RNA
Publication date: Available online 9 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Dana Abou Samhadaneh, Khalid A. Alqarni, Adam Smart, Mohuizi Kuang, Ossama Moujaber, Dusica Maysinger, Ursula StochajAbstractGold nanoparticles have excellent potential for theranostic applications, but their impact on living cells is only partially understood. Many gold nanoparticles enter cells through endosomes/lysosomes which are linked to different cell organelles and compartments. Our study focuses on the unfolded protein response (UPR) in the endoplasmic reticulum (ER), cytoplasmic RNA-granules and pr...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 11, 2019 Category: Nanotechnology Source Type: research

Multimodal molecular imaging of atherosclerosis: Nanoparticles functionalized with scFv fragments of an anti-αIIbβ3 antibody
In this study, scFv antibody fragments were used to functionalize multimodal imaging nanoparticles. This grafting was performed in a regio-selective way to preserve TEG4 activity and the avidity of the nanoparticles was studied with respect to the number of grafted antibodies. Subsequently, taking advantage of the nanoparticle bimodality, both near infrared fluorescence and magnetic resonance imaging of the atheroma plaque were performed in the ApoE-/- mouse model. Here we describe the design of the targeted nanoparticles, and a quantification method for their detection in mice, both ex vivo and in vivo, highlighting their...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 11, 2019 Category: Nanotechnology Source Type: research

Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats
Publication date: Available online 7 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Chi Zhang, Peichun Hsu, Dimin Wang, Wei Zhang, Changqing Zhang, Shang Guo, Weichao Yang, Xiaokang Wei, Yang Zhang, Biao ZhongAbstractHeterotopic ossification (HO) is a painful disease characterized by unwanted bone ectopic formation outside of the skeleton after injury. SPIO nanoparticles therapy has been widespreadly used in diverse orthopedic diseases. However, the effect of SPIO nanoparticles on heterotopic ossification remains unknown. Here, we prepared the SPIO nanoparticles carrying mothers against dec...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 8, 2019 Category: Nanotechnology Source Type: research

Tethering peptides onto biomimetic and injectable nanofiber microspheres to direct cellular response
Publication date: Available online 7 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Johnson V. John, Meera Choksi, Shixuan Chen, Sunil Kumar Boda, Yajuan Su, Alec McCarthy, Matthew J. Teusink, Richard A. Reinhardt, Jingwei XieAbstractBiomimetic and injectable nanofiber microspheres (NMs) could be ideal candidate for minimally invasive tissue repair. Herein, we report a facile approach to fabricate peptide-tethered NMs by combining electrospinning, electrospraying, and surface conjugation techniques. The composition and size of NMs can be tuned by varying the processing parameters during the...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 8, 2019 Category: Nanotechnology Source Type: research

Single-Cell Kinetics of siRNA-Mediated mRNA Degradation
Publication date: Available online 8 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Rafał Krzysztoń, Daniel Woschée, Anita Reiser, Gerlinde Schwake, Helmut H. Strey, Joachim O. RädlerAbstractRNA interference (RNAi) enables the therapeutic use of small interfering RNAs (siRNAs) to silence disease-related genes. The efficiency of silencing is commonly assessed by measuring expression levels of the target protein at a given time point post-transfection. Here, we determine the siRNA-induced fold change in mRNA degradation kinetics from single-cell fluorescence time-courses obtaine...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 8, 2019 Category: Nanotechnology Source Type: research

Pretargeted delivery of PEG-coated drug carriers to breast tumors using multivalent, bispecific antibody against polyethylene glycol and HER2
Publication date: Available online 5 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Christina L. Parker, Morgan D. McSweeney, Andrew T. Lucas, Timothy M. Jacobs, Daniel Wadsworth, William C. Zamboni, Samuel K. LaiAbstractPretargeting is an increasingly explored strategy to improve nanoparticle targeting, in which pretargeting molecules that bind both selected epitopes on target cells and nanocarriers are first administered, followed by the drug-loaded nanocarriers. Bispecific antibodies (bsAb) represent a promising class of pretargeting molecules, but how different bsAb formats may impact t...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 6, 2019 Category: Nanotechnology Source Type: research

Functional lipid polymeric nanoparticles for oral drug delivery: Rapid mucus penetration and improved cell entry and cellular transport
This study identified the key factors involved in oral bioavailability enhancement.Graphical AbstractThe hydrophilic copolymer pHPMA associated with vitamin B12-modified chitosan-covered lipid polymeric nanoparticles (H/VC-LPNs) releases the pHPMA during mucus penetration. Endocytosis then occurs via the VB12 receptor along with caveolae and the caveolate-mediated route. The nanoparticles promote transepithelial absorption via paracellular transport and open tight junctions between intestinal cells. They also enter M-cells in Peyer's patches, thereby creating an alternate absorption route for drug delivery. Rapid mucus per...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 2, 2019 Category: Nanotechnology Source Type: research

Tumor penetration of Sub-10 nm nanoparticles: effect of dendrimer properties on their penetration in multicellular tumor spheroids
Publication date: Available online 13 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Jason Bugno, Michael J. Poellmann, Karol Sokolowski, Hao-jui Hsu, Dong-Hwan Kim, Seungpyo HongAbstractUltrasmall nanoparticles (NPs,
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 1, 2019 Category: Nanotechnology Source Type: research

Gd-encapsulated carbonaceous dots for accurate characterization of tumor vessel permeability in magnetic resonance imaging
Publication date: Available online 31 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Yongyi Wu, Yuling Yan, Xuan Gao, Lili Yang, Ying Li, Xiaolu Guo, Jin Xie, Kai Wang, Xilin SunAbstractThe assessment of vascular permeability of malignant tumor plays an important role in the diagnosis and treatment of cancer. Dynamic contrast enhanced magnetic resonance image (DCE-MRI) using Gd-encapsulated carbonaceous dots and Gd-DTPA-BMA as contrast agents was performed in 4 T1 mouse breast cancer and HCC827 human non-small–cell lung cancer (NSNLC) xenograft models. Histopathological parameters of tu...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 1, 2019 Category: Nanotechnology Source Type: research

Preferential uptake of chitosan-coated PLGA nanoparticles by primary human antigen presenting cells
Publication date: Available online 31 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Verónica Durán, Hanzey Yasar, Jennifer Becker, Durairaj Thiyagarajan, Brigitta Loretz, Ulrich Kalinke, Claus-Michael LehrAbstractBiodegradable polymeric nanoparticles (NP) made from poly (lactid-co-glycolide) acid (PLGA) and chitosan (CS) hold promise as innovative formulations for targeted delivery. Since interactions of such NP with primary human immune cells have not been characterized, yet, here we assessed the effect of PLGA or CS-PLGA NP treatment on human peripheral blood mononuclear cell...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 1, 2019 Category: Nanotechnology Source Type: research

Differential in vivo biodistribution of 131I-labeled exosomes from diverse cellular origins and its implication for theranostic application
Publication date: Available online 1 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mohammad H. Rashid, Thaiz F. Borin, Roxan Ara, Kartik Angara, Jingwen Cai, Bhagelu R. Achyut, Yutao Liu, Ali S. ArbabAbstractExosomes are critical mediators of intercellular crosstalk and are regulator of the cellular/tumor microenvironment. Exosomes have great prospects for clinical application as a theranostic and prognostic probe. Nevertheless, the advancement of exosomes research has been thwarted by our limited knowledge of the most efficient isolation method and their in vivo trafficking. Here we have ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 1, 2019 Category: Nanotechnology Source Type: research

Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles
Publication date: Available online 30 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ruoning Wang, Jinyu Huang, Jian Chen, Mengmeng Yang, Honglan Wang, Hongzhi Qiao, Zhipeng Chen, Lihong Hu, Liuqing Di, Junsong LiAbstractColon adenocarcinoma is the third most common cause of cancer-related deaths worldwide owing to its aggressive nature. Here, we developed a novel oral drug delivery system (DDS) that comprised active targeted nanoparticles made from gelatin and chitosan (non-toxic polymers). The nanoparticles were fabricated using a complex coacervation method, which was accompanied by conjug...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 31, 2019 Category: Nanotechnology Source Type: research

Silver nanoparticles alter epithelial basement membrane integrity, cell adhesion molecule expression, and TGF-β1 secretion
This study analyzed the effects of AgNPs on BM production, composition and integrin/focal adhesion interactions in representative lung, esophageal, breast and colorectal epithelia models. A multidisciplinary approach including focused proteomics, QPCR arrays, pathway analyses, and immune-based, structural and functional assays was used to identify molecular and physiological changes in cell adhesions and the BM induced by acute and chronic AgNP exposure. Dysregulated targets included CD44 and transforming growth factor-beta, two proteins frequently altered during pathogenesis. Results indicate AgNP exposure interferes with...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 26, 2019 Category: Nanotechnology Source Type: research

Iron oxide nanoparticles in liquid or powder form enhanced osteogenesis via stem cells on injectable calcium phosphate scaffold
In conclusion, incorporating IONPs in liquid or powder form both substantially enhanced hDPSCs on IONP-CPC scaffold and exhibited excellent biocompatibility. IONP incorporation as a liquid was better than IONP powder in promoting osteogenic differentiation of hDPSCs. Incorporating IONPs and chitosan lactate together in CPC enhanced osteogenesis of hDPSCs more than using either alone.Graphical AbstractIron oxide nanoparticles (IONPs) which can be incorporated as liquid or powder can enhance the bioactivity of calcium phosphate cements (CPCs). However, incorporation methods can affect the bioactivity of the final IONP-CPC. A...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 26, 2019 Category: Nanotechnology Source Type: research

Redox dual-responsive paclitaxel-doxorubicin heterodimeric prodrug self-delivery nanoaggregates for more effective breast cancer synergistic combination chemotherapy
Publication date: Available online 24 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Yingli Wang, Jiamei Wang, Liyuan Yang, Wei Wei, Bingjun Sun, Kexin Na, Yuxi Song, Haotian Zhang, Zhonggui He, Jin Sun, Yongjun WangAbstractA single nanodrug delivery system for combined delivery of paclitaxel and doxorubicin that integrates high co-loading efficiency, synchronous co-delivery of combined drugs, controllable drug release, and maintains the drug combination at fixed synergistic ratios has been proven to be challenging. Here, we report a redox dual-responsive prodrug nanosystem consisting of a pa...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 26, 2019 Category: Nanotechnology Source Type: research

Magnetic Nanorods for remote disruption of lipid membranes by non-heating low frequency magnetic field
Publication date: Available online 23 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Irina M. Le-Deygen, Kseniya Yu. Vlasova, Ekateriana O. Kutsenok, Azizbek D. Usvaliev, Maria V. Efremova, Alexander O. Zhigachev, Polina G. Rudakovskaya, Dmitry Yu. Golovin, Sergey L. Gribanovsky, Elena V. Kudryashova, Alexander G. Majouga, Yury I. Golovin, Alexander V. Kabanov, Natalia L. KlyachkoAbstractThis work presents direct evidence of disordering of liposomal membranes by magnetic nanoparticles during their exposures to non-heating alternating Extremely Low Frequency Magnetic Field (ELF MF). Changes in...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 24, 2019 Category: Nanotechnology Source Type: research

Towards a nanoparticle-based prophylactic for maternal autoantibody-related autism
Publication date: Available online 23 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): B.S Amir Bolandparvaz, B.S Rian Harriman, Kenneth Alvarez, Kristina Lilova, Zexi Zang, B.S. Andy Lam, Elizabeth Edmiston, Alexandra Navrotsky, Natalia Vapniarsky-Arzi, Judy Van De Water, Jamal S. LewisAbstractRecently, the causative agents of Maternal Autoantibody-Related (MAR) autism, pathological autoantibodies and their epitopic targets (e.g. lactate dehydrogenase B [LDH B] peptide), have been identified. Herein, we report on the development of Systems for Nanoparticle-based Autoantibody Reception and Entr...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 24, 2019 Category: Nanotechnology Source Type: research

Engineered nanomedicine for neuroregeneration: Light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor
Publication date: Available online 23 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Muzhaozi Yuan, Ya Wang, Yi-Xian QinAbstractThis paper reports nerve growth factor functionalized superparamagnetic iron oxide–gold core-shell nanoparticles (NGF-SPIO-Au NPs), an engineered nanomedicine for non-invasive neuron regeneration when irradiated by a low-intensity light-emitting diode (LED). NGF-SPIO-Au NPs of 20 μg/ml, were tested on PC-12 neuron-like cells, irradiated by LEDs (525 nm, 1.09, 1.44, and 1.90 mW/cm2). A remarkable Ca2+ influx was detected in differentiated PC-12...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 24, 2019 Category: Nanotechnology Source Type: research

Drug-binding albumins forming stabilized nanoparticles for efficient anticancer therapy
Publication date: Available online 22 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Jianxiang Huang, Bihan Wu, Zhuxian Zhou, Shiqi Hu, Hongxia Xu, Ying Piao, Hao Zheng, Jianbin Tang, Xiangrui Liu, Youqing ShenAbstractAlbumin is a serum transport protein, which has been utilized as a carrier for a variety of drugs to improve their delivery efficiency and to obtain favorable pharmacokinetic profiles. However, natural albumin possesses only a few high-affinity binding sites for a limited number of drugs. This results in deficiencies in drug-loading and serum stability, and consequently, in impa...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 23, 2019 Category: Nanotechnology Source Type: research

Targeted and effective glioblastoma therapy via aptamer-modified tetrahedral framework nucleic acid-paclitaxel Nanoconjugates that can pass the blood brain barrier
Publication date: Available online 22 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Sirong Shi, Wei Fu, Shiyu Lin, Taoran Tian, Songhang Li, Xiaoru Shao, Yuxin Zhang, Tao Zhang, Zisheng Tang, Yi Zhou, Yunfeng Lin, Xiaoxiao CaiAbstractTargeted DNA nanoparticles have been identified as one of the most promising nanocarriers in anti-glioma drug delivery. We established a multifunctional nanosystem for targeted glioma therapy. Tetrahedral framework nucleic acid (tFNA), entering U87MG cells and bEnd.3 cells, was chosen to deliver two aptamers, GMT8 and Gint4.T, and paclitaxel. GMT8 and Gint4.T, w...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 23, 2019 Category: Nanotechnology Source Type: research

Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies
Publication date: Available online 22 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Gayong Shim, Jinwon Park, Mi-Gyeong Kim, Geon Yang, Yeon Lee, Yu-Kyoung OhAbstractHere, we report various therapeutic cargo-loadable DNA nanostructures that are shelled in polydopamine and noncovalently tethered with cancer cell-targeting DNA aptamers. Initial DNA nanostructure was formed by rolling-circle amplification and condensation with Mu peptides. This DNA nanostructure was loaded with an antisense oligonucleotide, a photosensitizer, or an anticancer chemotherapeutic drug. Each therapeutic agent-loaded...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 23, 2019 Category: Nanotechnology Source Type: research

Highly biocompatible thermosensitive nanocomposite gel for combined therapy of hepatocellular carcinoma via the enhancement of mitochondria related apoptosis
Publication date: Available online 23 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Hao Fu, Lili Huang, Chaoqun Xu, Jiali Zhang, Dongxiao Li, Li Ding, Lei Liu, Yi Dong, Wenping Wang, Yourong DuanAbstractPrimary hepatocellular carcinoma (HCC) is a common malignant tumor. Surgery is the main treatment, but HCC patients have a potential risk of tumor recurrence. Besides, many limitations arise during the application of single first-line antitumor drugs. Here, we selected Pluronic F-127 and sodium alginate (SA) to prepare a thermosensitive gel (Gel). The optimal synergistic ratio of PTX and DOX ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 23, 2019 Category: Nanotechnology Source Type: research

AS1411-conjugated gold nanoparticles affect cell proliferation through a mechanism that seems independent of nucleolin
Publication date: Available online 20 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Samaneh Kabirian-Dehkordi, Mounira Chalabi-Dchar, Hichem C Mertani, Dominique Le Guellec, Bernard Verrier, Jean-Jacques Diaz, Masoud A. Mehrgardi, Philippe BouvetAbstractG-rich oligonucleotide, AS1411, has been shown to interact with nucleolin and to inhibit cancer cell proliferation and tumor growth. This antiproliferative action is increased when AS1411 is conjugated to different types of nanoparticles. However, the molecular mechanisms are not known. In this work, we show in several cell lines that optimiz...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 21, 2019 Category: Nanotechnology Source Type: research

Subcutaneous vaccination using injectable biodegradable hydrogels for long-term immune response
Publication date: Available online 20 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ashlynn L.Z. Lee, Chuan Yang, Shujun Gao, James L. Hedrick, Yi Yan YangAbstractProlonged vaccine release enables gradual immunostimulation, providing long-term immunity. Herein, Vitamin E- PEG-Vitamin E triblock ‘ABA’ hydrogel, which is formed through physical cross-linking of flower-shaped micelles and can reside in vivo for>17 weeks, was employed for delivery of cancer preventive vaccines to provide sustained anticancer immunity. Mice vaccinated with hydrogel formulations produced a sign...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 21, 2019 Category: Nanotechnology Source Type: research

Nanotherapeutic silibinin: An insight of phytomedicine in healthcare reformation
Publication date: Available online 21 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Anjali Takke, Pravin ShendeAbstractMost of the herbal origin drugs possess water insoluble active constituents which lower the bioavailability and increase systemic clearance after administration of repeated or higher dose of drug. Silymarin is extracted from the seeds and fruits of milk thistle plant Silybum marianum which consists of main biologically active component as silibinin. However, the clinical applications of silibinin show some limitations due to low aqueous solubility, poor penetration into the ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 21, 2019 Category: Nanotechnology Source Type: research

Polyethylenimine-coated superparamagnetic iron oxide nanoparticles impair in vitro and in vivo angiogenesis
Publication date: Available online 18 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Vladimir Mulens-Arias, José Manuel Rojas, Laura Sanz-Ortega, Yadileiny Portilla, Sonia Pérez-Yagüe, Domingo F. BarberAbstractEndothelial cells are essential to tumor vascularization and impairing their activity can potentially limit tumor growth. Since polyethylenimine (PEI)-coated superparamagnetic iron oxide nanoparticles (SPIONs) are bioactive nanosystems that modulate inflammatory macrophage responses and limit tumor cell invasion, we evaluated their effects on endothelial cell angiogen...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 20, 2019 Category: Nanotechnology Source Type: research

MRI reporter gene MagA suppresses transferrin receptor and maps Fe2+ dependent lung cancer
Publication date: Available online 18 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiaoying Guan, Bin Yang, Maobin Xie, Deependra Kumar Ban, Xinmin Zhao, Ratnesh Lal, Feng ZhangAbstractAs a magnetic resonance imaging (MRI) reporter gene, MagA has become a powerful tool to monitor dynamic gene expression and allowed concomitant high resolution anatomical and functional imaging of subcellular genetic information. Here we establish a stably expressed MagA method for lung cancer MRI. The results show that MagA cannot only enhance both in vitro and in vivo MRI contrast by specifically alternatin...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 20, 2019 Category: Nanotechnology Source Type: research

Pentaerythritol-based lipid A bolsters the antitumor efficacy of a polyanhydride particle-based cancer vaccine
Publication date: Available online 15 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Emad I. Wafa, Sean M. Geary, Kathleen A. Ross, Jonathan T. Goodman, Balaji Narasimhan, Aliasger K. SalemAbstractThe primary objective of this study was to enhance the antitumor efficacy of a model cancer vaccine through co-delivery of pentaerythritol lipid A (PELA), an immunological adjuvant, and a model tumor antigen, ovalbumin (OVA), separately loaded into polyanhydride particles (PA). In vitro experiments showed that encapsulation of PELA into PA (PA-PELA) significantly enhanced its stimulatory capacity on...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 15, 2019 Category: Nanotechnology Source Type: research

Osteoclasts and tumor cells dual targeting nanoparticle to treat bone metastases of lung cancer
Publication date: Available online 13 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Shao-bo Bai, Dao-zhou Liu, Ying Cheng, Han Cui, Miao Liu, Min-xuan Cui, Bang-le Zhang, Qi-bing Mei, Si-yuan ZhouAbstractBone is one of the prone metastatic sites of lung cancer. Osteoclast plays an important role in bone resorption and the growth of bone metastases of lung cancer. In order to treat bone metastases of lung cancer, we reported a docetaxel (DTX)-loaded nanoparticle, DTX@AHP, which could target dually at osteoclasts and bone metastatic tumor cells. The in vitro drug release from DTX@AHP exhibited...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 14, 2019 Category: Nanotechnology Source Type: research

Tumor penetration of Sub-10 nm nanoparticles: Effect of dendrimer properties on their penetration in multicellular tumor spheroids
Publication date: Available online 13 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Jason Bugno, Michael J. Poellmann, Karol Sokolowski, Hao-jui Hsu, Dong-Hwan Kim, Seungpyo HongAbstractUltrasmall nanoparticles (NPs,
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 14, 2019 Category: Nanotechnology Source Type: research

An innovative in situ method of creating hybrid dendrimer nano-assembly: An efficient next generation dendritic platform for drug delivery
We report an easy in situ method of creating hybrid dendrimer nano-assembly by mixing G4.0 PAMAM (–NH2) and G3.5 PAMAM (–COONa) dendrimers with a chemotherapeutic drug docetaxel (DTX). Zeta potential, HR-TEM, 1H-NMR proved the formation of nano-assembly. In vitro dissolution, release studies revealed pH dependent dissolution and sustained drug release. Cellular uptake, cytotoxicity, and flow cytometric analysis in human/mouse glioblastoma cells indicated the effectiveness of hybrid dendrimers. The oral administration of the hybrid dendrimers showed pharmacokinetic equivalence to intravenous injection of commerc...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 11, 2019 Category: Nanotechnology Source Type: research

Evaluation of an adjuvanted hydrogel-based pDNA nanoparticulate vaccine for rabies prevention and immunocontraception
In this study, we developed an adjuvanted hydrogel-based pDNA nanoparticulate vaccine for rabies protection and immunocontraception. In vivo, we observed an immune response skewed toward a Th2 type, in contrast to the Th1 type in our previous pDNA study. The observation was verified by the IgG2a/IgG1 ratio (
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 5, 2019 Category: Nanotechnology Source Type: research