γH2AX is increased in peripheral blood lymphocytes of Alzheimer’s disease patients in the South Australian Neurodegeneration, Nutrition and DNA Damage (SAND) study of aging
In this study, the endogenous γH2AX level was measured, using laser scanning cytometry (LSC) and visual scoring, in lymphocyte nuclei from MCI (n = 18), or AD (n = 20) patients and healthy controls (n = 40). Levels were significantly elevated in nuclei of the AD group compared to the MCI and control groups, and there was a concomitant increase, with a significant trend, from the control group through MCI to the AD group. A significant negative correlation was seen between γH2AX and the mini mental state examination (MMSE) score, when the analysis included all subjects. Receiver Operation Characteris...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 14, 2018 Category: Genetics & Stem Cells Source Type: research

Genetic polymorphisms of IL17A, TLR4 and P2RX7 and associations with the risk of chronic obstructive pulmonary disease
Publication date: May–June 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 829–830 Author(s): Yaoyao Dai, Zhihai Zhang, Lina Xu, Yunfei Shang, Rongrong Lu, Jie Chen We investigated the association between genetic polymorphisms of IL17A, TLR4 and P2RX7 genes and chronic obstructive pulmonary disease (COPD) in a Han population. We performed a case-control study with 152 COPD subjects from the Third People’s Hospital of Nantong in 2015. Healthy controls were selected from a group of people attending the physical examination and were frequency-matched to the cases by s...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 14, 2018 Category: Genetics & Stem Cells Source Type: research

Genotoxicity evaluation of multi-component mixtures of polyaromatic hydrocarbons (PAHs), arsenic, cadmium, and lead using flow cytometry based micronucleus test in HepG2 cells
In this study, we have evaluated the combined genotoxicity, AhR activity and cell cycle parameters of four PAHs (benzo[a]pyrene (Ba]P), naphthalene (Nap), phenanthrene (Phe) and pyrene (Pyr)) and three metals (arsenic (As), cadmium (Cd), and lead (Pb)) in HepG2 cells using a flow cytometry based micronucleus (MN) test CAFLUX assay and nuclear fluorescence assay, respectively. The mixtures of B[a]P and metals induced a maximum of four fold increase in the MN formation compared to B[a]P alone. The higher combination of PAHs and metals did not significantly increase the MN formation. The mixtures of metals or non-carcinogenic...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 10, 2018 Category: Genetics & Stem Cells Source Type: research

Cypermethrin: oxidative stress and genotoxicity in retinal cells of the adult zebrafish
Publication date: Available online 27 December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): E.V. Paravani, M.F. Simoniello, G.L. Poletta, F.R. Zolessi, V.H. Casco Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminants in freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD) and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show the presence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 28, 2017 Category: Genetics & Stem Cells Source Type: research

Aspirin and ibuprofen, in bulk and nanoforms: Effects on DNA damage in peripheral lymphocytes from breast cancer patients and healthy individuals
Publication date: Available online 24 December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Osama Dandah, Mojgan Najafzadeh, Mohammed Isreb, Richard Linforth, Catherine Tait, Adi Baumgartner, Diana Anderson Regular use of non-steroidal anti-inflammatory drugs (NSAIDs) may be protective against tumours, including breast cancer. We have studied the effects of ibuprofen and aspirin on DNA damage in lymphocytes obtained from breast cancer patients and healthy female controls. Both nanoparticle (NPs) and bulk formulations were used in the comet and micronucleus (MN) assays. Non-toxi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 24, 2017 Category: Genetics & Stem Cells Source Type: research

Assessing the impact of benzo[a]pyrene with the in vitro fish gut model: an integrated approach for eco-genotoxicological studies
Publication date: Available online 24 December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Laura M. Langan, Silvia Arossa, Stewart F. Owen, Awadhesh N. Jha In vitro models are emerging tools for reducing reliance on traditional toxicity tests, especially in areas where information is sparse. For studies of fish, this is especially important for extrahepatic organs, such as the intestine, which, until recently, have been largely overlooked in favour of the liver or gill. Considering the importance of dietary uptake of contaminants, the rainbow trout (Oncorhynchus mykiss) intest...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 24, 2017 Category: Genetics & Stem Cells Source Type: research

In vivo antimutagenic and antiatherogenic effects of the (1  → 3)(1 → 6)-β-d- glucan botryosphaeran
Publication date: February 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 826 Author(s): Geralda Gillian Silva-Sena, Maressa Malini, Juliana Macedo Delarmelina, Jean Carlos Vencioneck Dutra, Suiany Vitorino Gervásio, Marcos André Soares Leal, Thiago de Melo Costa Pereira, Aneli M. Barbosa-Dekker, Robert F.H. Dekker, Flavia de Paula, Maria do Carmo Pimentel Batitucci The antimutagenic effect of botryosphaeran, an exocellular (1 → 3)(1 → 6)-β-d-glucan, from the ascomyceteous and plant-borne endophytic fungus, Botryosphaeria rhodina MAMB-05, was eva...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 24, 2017 Category: Genetics & Stem Cells Source Type: research

In vivo antimutagenic and antiatherogenic effects of the (1 →3)(1→6)-β-D- glucan botryosphaeran
Publication date: Available online 21 December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Geralda Gillian Silva-Sena, Maressa Malini, Juliana Macedo Delarmelina, Jean Carlos Vencioneck Dutra, Suiany Vitorino Gervásio, Marcos André Soares Leal, Thiago de Melo Costa Pereira, Aneli M. Barbosa-Dekker, Robert F.H. Dekker, Flavia de Paula, Maria do Carmo Pimentel Batitucci The antimutagenic effect of botryosphaeran, an exocellular (1 → 3)(1 → 6)-β-D-glucan, from the ascomyceteous and plant-borne endophytic fungus, Botryosphaeria rhodina MAMB-05,...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 22, 2017 Category: Genetics & Stem Cells Source Type: research

Interactive potential of genetic polymorphism in Xenobiotic metabolising and DNA repair genes for predicting lung cancer predisposition and overall survival in North Indians.
Conclusion Through the study we introduced the concept of polygenic approach to get an insight about the various polymorphic variants in determining cancer susceptibility. Lesser number of subjects were found in the high risk subgroups. Further studies with larger sample size are required to warranty the above findings. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 22, 2017 Category: Genetics & Stem Cells Source Type: research

EVALUATION OF Pb-210 IN URINE AND FREQUENCY OF MICRONUCLEI IN EXFOLIATED CELLS AS INDICATORS OF EXPOSURE TO CIGARETTES
This study aimed at analyzing the frequency of micronuclei (MN) in exfoliated cells as well as the levels of Pb-210 in urine samples to evaluate the association between the smoking habit and toxic stress of transitional epithelial cells. The frequency of MN was scored from Giemsa-stained slides while exchange resin and beta counting techniques were employed to measure the concentrations of this radioisotope. Urine samples of smokers had levels of Pb-210 up to 158.65 mBq L−1. For nonsmokers, the median was below the detection limit (45 mBq L−1). The analyses of mononucleated cells showed a significant in...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 19, 2017 Category: Genetics & Stem Cells Source Type: research

Folate modulates guanine-quadruplex frequency and DNA damage in Werner syndrome
Publication date: Available online 9 December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Paniz Tavakoli Shirazi, Wayne Richard Leifert, Michael Felix Fenech, Maxime François Guanine-quadruplexes (G4) are stable tetra-stranded DNA structures that may cause DNA replication stress and inhibit gene expression. Defects in unwinding these structures by DNA helicases may result in telomere shortening and DNA damage. Furthermore, due to mutations in WRN helicase genes in Werner syndrome, G4 motifs are likely to be key elements in the expression of premature aging phenotypes. T...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2017 Category: Genetics & Stem Cells Source Type: research

Flavonoid Fraction of Cajanus cajan Prohibited The Mutagenic Properties of Cyclophosphamide in Mice In Vivo
In conclusion, the flavonoid extract improved cell viability and protected animal cells from the cytotoxic and genotoxic effects exhibited by CP. Cajanus cajan flavonoids might contain the antioxidant bioactivity that effectively lessened chromosome aberrations and DNA damage induced by mutagenic agents. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2017 Category: Genetics & Stem Cells Source Type: research

A special issue dedicated to Dr. David Michael DeMarini: Introduction
Publication date: Available online 13 December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Larry D. Claxton (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2017 Category: Genetics & Stem Cells Source Type: research

A three-dimensional in vitro HepG2 cells liver spheroid model for genotoxicity studies
This study reports on the development of a 3D liver model, using HepG2 cells, by a hanging-drop technique, with a focus on evaluating spheroid growth characteristics and suitability for genotoxicity testing. The cytokinesis-blocked micronucleus assay protocol was adapted to enable micronucleus (MN) detection in the 3D spheroid models. This involved evaluating the difference between hanging vs non-hanging drop positions for dosing of the test agents and comparison of automated Metafer scoring with manual scoring for MN detection in HepG2 spheroids. The initial seeding density, used for all experiments, was 5000 cells/20 &...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2017 Category: Genetics & Stem Cells Source Type: research

Synthesis and evaluation of the mutagenicity of 3-alkylpyridine marine alkaloid analogues with anticancer potential
Publication date: January 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 825 Author(s): Maria Cristina S. Barbosa, Camila de Souza Barbosa, Júlia T. de Oliveira, Natália Chermont S. Moreira, Natália Rezende de Miranda Martins, Gabrielle K. Alves Gomes, Camila A. Caldeira, Marília Ladeira Alves e Costa, Daniel Silqueira Martins Guimarães, Luciana Guimarães, Clebio S. Nascimento, Fernando de Pilla Varotti, Gustavo Henrique Ribeiro Viana, Fabio Vieira dos Santos Theonella sp is an important source of biologically–active 3-alkylpyridine al...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2017 Category: Genetics & Stem Cells Source Type: research

DNA damage in rats with streptozotocin-induced diabetes; protective effect of silibinin
Publication date: January 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 825 Author(s): Vehbi Atahan Toğay, Tuğba Semerci Sevimli, Murat Sevimli, Dilek Aşcı Çelik, Nurten Özçelik Silibinin, the active component of Silybum marianum (L.), is a powerful antioxidant. Male rats with streptozotocin-induced diabetes were treated with silibinin. DNA damage was demonstrated by the comet assay in the control, diabetic, and treatment groups. DNA damage was increased in diabetic rats and decreased by silibinin treatment. (Source: Mutation Research Genetic Toxicology and E...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 16, 2017 Category: Genetics & Stem Cells Source Type: research

Corrigendum to “Relationship between spontaneous γH2AX foci formation and progenitor functions in circulating hematopoietic stem and progenitor cells among atomic-bomb survivors” [Mutat. Res. – Genet. Toxicol. Environ. Mutagen. 802 (2016) 59–65]
Publication date: Available online 10 November 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Junko Kajimura, Seishi Kyoizumi, Yoshiko Kubo, Munechika Misumi, Kengo Yoshida, Tomonori Hayashi, Kazue Imai, Waka Ohishi, Kei Nakachi, Nan-ping Weng, Lauren F. Young, Jae-Hung Shieh, Malcolm A. Moore, Marcel R.M. van den Brink, Yoichiro Kusunoki (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 10, 2017 Category: Genetics & Stem Cells Source Type: research

Analysis of negative historical control group data from the in vitro micronucleus assay using TK6 cells
This study demonstrates the value of QC methods in helping to analyse the reproducibility of results, building up a ‘normal’ range of values, and as an aid to identify variability within a laboratory in order to implement processes to maintain and improve uniformity. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 10, 2017 Category: Genetics & Stem Cells Source Type: research

Evaluation of chromosomal DNA damage, cytotoxicity, cytostasis, oxidative DNA damage and their relationship with endocrine hormones in patients with acute organophosphate poisoning
In conclusion, it is believed that this is the first study to evaluate the chromosomal/oxidative DNA damage, cell proliferation, cell death and their associations with endocrine hormones in acute OPs poisoning. These preliminary findings need to be supported by further studies with larger sample sizes. Graphical abstract (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 9, 2017 Category: Genetics & Stem Cells Source Type: research

Biphasic Regulation of Spindle Assembly Checkpoint by Low and High Concentrations of Resveratrol Leads to the Opposite Effect on Chromosomal Instability
In conclusion, RSV exhibited a biphasic dose-dependent effect on CIN that was exerted via the regulation of mitotic fidelity through the SAC network. The health implications of these findings were summarized. Graphical abstract (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 8, 2017 Category: Genetics & Stem Cells Source Type: research

The evaluation of dna damage in rats w İth streptozotocin induced diabetes and the protective effect of silibinin on dna damage
In this study, it was aimed to reveal possible DNA damage caused by diabetes and the possible protective effect of silibinin against DNA damage. Silibinin is the active component of Silybum marianum (L.) plant. Studies show that Silibinin is a powerful antioxidant and protective against free radicals. 56 male rats with streptozotocin-induced diabetes were treated with silibinin at different doses. DNA damage in control, diabetes and treatment groups was demonstrated by Comet assay. Statistical analysis of the Tail DNA Percentage parameter showed significant results. DNA damage was increased in diabetic rats, and was decrea...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 4, 2017 Category: Genetics & Stem Cells Source Type: research

The Backwater Clam (Meretrix casta) as a bioindicator species for monitoring the pollution of an estuarine environment by genotoxic agents
Publication date: Available online 4 November 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Avelyno H. D’costa, S.K. Shyama, Praveen Kumar M.K., Swizzle Furtado The coast of Goa receives anthropogenic stress through its major rivers, which carry mining wastes, including iron and manganese ores from upstream mining sites, and petroleum hydrocarbons from shipping activities. These contaminants show seasonal variation in concentration and may be bioaccumulated by fauna inhabiting these waters. These fauna, including the bivalve molluscs, are particularly at risk due to these ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 4, 2017 Category: Genetics & Stem Cells Source Type: research

Generation of Advanced Glycation End-Products (AGEs) by glycoxidation mediated by copper and ROS in a human serum albumin (HSA) model peptide: reaction mechanism and damage in motor neuron cells
Publication date: December 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 824 Author(s): Caroline Martins Sandanielo Marques, Emilene Arusievicz Nunes, Larissa Lago, Cibele Nicolaski Pedron, Tânia Maria Manieri, Roseli Hiromi Sato, Vani Xavier Oliveira, Giselle Cerchiaro Glucose, in the presence of reactive oxygen species (ROS), acts as an as an oxidative agent and drives deleterious processes in Diabetes Mellitus. We have studied the mechanism and the toxicological effects of glucose-dependent glycoxidation reactions driven by copper and ROS, using a model peptide based on th...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 1, 2017 Category: Genetics & Stem Cells Source Type: research

Cytogenetic changes in the Bhopal population exposed to methyl isocyanate (MIC) in 1984: Then and 30 years later
Publication date: Available online 13 October 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Bani Bandana Ganguly Following the 1984 Bhopal methyl isocyanate (MIC) gas disaster, genetic alterations were sporadically reported on small cohorts. However, the outcome of the multi-center cytogenetic screening conducted at that time remains unknown and no follow-up studies on the long-term effects of MIC exposure have been published. The present work examines genetic changes in the exposed population,with the aim of identifying any long-term effects of MIC. G-Banded metaphases were stu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 13, 2017 Category: Genetics & Stem Cells Source Type: research

DNA-dependent protein kinase modulates the anti-cancer properties of silver nanoparticles in human cancer cells
In this study, we demonstrate that Ag-np treatment trigger the activation of DNA-PKcs and JNK pathway at selected doses, presumably as a physiologic response to DNA damage and repair in normal and malignant cells. Ag-np altered the telomere dynamics by disrupting the shelterin complex located at the telomeres and telomere lengths. The genotoxic effect of Ag-np was not restricted to telomeres but the entire genome as Ag-np induced γ-H2AX foci formation, an indicator of global DNA damage. Inhibition of DNA-PKcs activity sensitised the cancer cells towards the cytotoxicity of Ag-np. Inhibition of DNA-PKcs substantiated ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 13, 2017 Category: Genetics & Stem Cells Source Type: research

Cytogenetic damage in peripheral blood lymphocytes of children exposed to pesticides in agricultural areas of the department of Cordoba, Colombia
Publication date: Available online 10 October 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Javier Alonso Ruiz-Guzmán, Pamela Gómez-Corrales, Ángel Cruz-Esquivel, José Luis Marrugo-Negrete Pesticides offer benefits, like optimization of agricultural production and disease control; however, these toxic substances can contaminate the environment and pose risks to human health. The aim of this study was to assess pesticide exposure and frequency of cytogenetic damage in infant populations in agricultural areas of the department of Córdoba, Colombi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 11, 2017 Category: Genetics & Stem Cells Source Type: research

Oxidative Stress and DNA Damage in the Cord Blood of Preterm Infants
In this study, we compared the DNA strand breaks as one of the results of DNA oxidation in white blood cells, malondialdehyde (oxidative stress marker), catalase and superoxide dismutase activity, and total antioxidant capacity (markers of antioxidant defense) in a cord blood plasma of a group of preterm (n=25) and full term births (n=25). The primary DNA damage and plasma oxidative stress markers were significantly higher in a preterm group (p<0.05). Cord plasma activity of superoxide dismutase was significantly lower in preterm infants (p≤0.001). However, there were no significant differences in the cord blood ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 10, 2017 Category: Genetics & Stem Cells Source Type: research

Induction of the chromosomal translocation t(14;18) by targeting the BCL-2 locus with specific binding I-125-labeled Triplex-Forming oligonucleotides
Publication date: Available online 14 September 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Volker Dahmen, Sabine Schmitz, Ralf Kriehuber Triplex-Forming oligonucleotides (TFO) bind sequence-specific to the DNA double helix in-vitro and in-vivo and are a promising tool to manipulate genes or gene regulatory elements. TFO as a carrier molecule for short-range particle emitter such as Auger-Electron-Emitters (AEE) bear the potential to introduce radiation-induced site-specific complex DNA lesions, which are known to induce chromosomal translocations. We studied gene expression, ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 14, 2017 Category: Genetics & Stem Cells Source Type: research

Metal bioaccumulation and mutagenesis in a Tradescantia clone following long-term exposure to soils from urban industrial areas and closed landfills
Publication date: Available online 12 September 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Tatjana Čėsnienė, Violeta Kleizaitė, Skaistė Bondzinskaitė, Ričardas Taraškevičius, Donatas Žvingila, Raimondas Šiukšta, Vytautas Rančelis Soil mutagens, particularly metals, may persist long after the source of pollution has been removed, representing a hazard to plants, animals, and humans in or near contaminated areas. Often, due to urban growth, previous land uses may be forgotten and hazards overlooked. We exposed Tradescantia clone #4430 plants to so...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 13, 2017 Category: Genetics & Stem Cells Source Type: research

Spectrum of benzo[a]pyrene-induced mutations in the Pig-a gene of L5178YTk+/ − cells identified with next generation sequencing
Publication date: Available online 13 September 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Javier Revollo, Yiying Wang, Page McKinzie, Azra Dad, Mason Pearce, Robert H. Heflich, Vasily N. Dobrovolsky We used Sanger sequencing and next generation sequencing (NGS) for analysis of mutations in the endogenous X-linked Pig-a gene of clonally expanded L5178YTk +/− cells. The clones developed from single cells that were sorted on a flow cytometer based upon the expression pattern of the GPI-anchored marker, CD90, on their surface. CD90-deficient and CD90-proficient cells were ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 13, 2017 Category: Genetics & Stem Cells Source Type: research

Ten factors for considering the mode of action of Cr(VI)-induced gastrointestinal tumors in rodents
Publication date: November 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 823 Author(s): Chad M. Thompson, Mina Suh, Deborah M. Proctor, Laurie C. Haws, Mark A. Harris The determination of whether a chemical induces a specific cancer through a mutagenic or non-mutagenic mode of action (MOA) plays an important role in choosing between linear and nonlinear low-dose extrapolation to derive toxicity criteria. There is no formal framework from the U.S. EPA for determining whether environmental chemicals act through a mutagenic or non-mutagenic MOA; consequently, most such determinations ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 13, 2017 Category: Genetics & Stem Cells Source Type: research

Multi-walled carbon nanotube-induced genotoxic, inflammatory and pro-fibrotic responses in mice: investigating the mechanisms of pulmonary carcinogenesis
In this study, the genotoxic, mutagenic, inflammatory, and fibrotic potential of MWCNTs were investigated. Muta™Mouse adult females were exposed to 36±6 or 109±18μg/mouse of Mitsui-7, or 26±2 or 78±5μg/mouse of NM-401, once a week for four consecutive weeks via intratracheal instillations, alongside vehicle-treated controls. Samples were collected 90days following the first exposure for measurement of DNA strand breaks, lacZ mutant frequency, p53 expression, cell proliferation, lung inflammation, histopathology, and changes in global gene expression. Both MWCNT types persisted in lun...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 9, 2017 Category: Genetics & Stem Cells Source Type: research

No evidence of DNA damage by co-exposure to extremely low frequency magnetic fields and aluminum on neuroblastoma cell lines
Publication date: Available online 4 September 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Milena Villarini, Angela Gambelunghe, Daniela Giustarini, Maria Vittoria Ambrosini, Cristina Fatigoni, Ranieri Rossi, Luca Dominici, Sara Levorato, Giacomo Muzi, Danilo Piobbico, Giuseppina Mariucci Whether exposure to 50–60Hz extremely low frequency magnetic fields (ELF-MF) exerts neurotoxic effects is a debated issue. Analogously, the potential role of Aluminum (Al) in neurodegeneration is a matter of controversial debate. As all living organisms are exposed to ELF-MF and/or Al d...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 5, 2017 Category: Genetics & Stem Cells Source Type: research

Effects of Pyridostigmine bromide on SH-SY5Y cells: an in vitro neuroblastoma neurotoxicity model
Publication date: Available online 30 August 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Veronica Farina Azzolin, Fernanda Barbisan, Luana Suéling Lenz, Cibele Ferreira Teixeira, Milena Fortuna, Thiago Duarte, Marta Maria Frescura Medeiros Duarte, Ivana Beatrice Mânica da Cruz Pyridostigmine bromide (PB) is a reversible acetylcholinesterase (AChE) inhibitor and the first-choice for the treatment of symptoms associated with myasthenia gravis and other neuromuscular junction disorders. However, evidence suggested that PB could be associated with the Gulf War Illness...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - August 31, 2017 Category: Genetics & Stem Cells Source Type: research

In vitro cell transformation induced by synthetic amorphous silica nanoparticles
In conclusion, this study showed for the first time the transforming potential of different SAS, which act as tumor-promoter substances in the Bhas 42 model of cell transformation. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - August 25, 2017 Category: Genetics & Stem Cells Source Type: research

Is mobile phone radiation genotoxic? An analysis of micronucleus frequency in exfoliated buccal cells
Publication date: Available online 5 August 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): F. Mendes de Oliveira, A.M. Carmona, C. Ladeira Electromagnetic fields (EMF) are classified as “possibly carcinogenic” by the International Agency for Research on Cancer (IARC). Some publications have reported associations between EMF exposure and DNA damage, but many other studies contradict such findings. Cytomorphological changes, such as micronuclei (MN), indicative of genomic damage, are biomarkers of genotoxicity. To test whether mobile phone-associated EMF exposure affect...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - August 5, 2017 Category: Genetics & Stem Cells Source Type: research

Genotoxicity testing: Comparison of the γH2AX focus assay with the alkaline and neutral comet assays
Publication date: Available online 22 July 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Teodora Nikolova, Federico Marini, Bernd Kaina Genotoxicity testing relies on the quantitative measurement of adverse effects, such as chromosome aberrations, micronuclei, and mutations, resulting from primary DNA damage. Ideally, assays will detect DNA damage and cellular responses with high sensitivity, reliability, and throughput. Several novel genotoxicity assays may fulfill these requirements, including the comet assay and the more recently developed γH2AX assay. Although they are...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 23, 2017 Category: Genetics & Stem Cells Source Type: research

Sensitivity and specificity prediction of the buccal micronucleus cytome assay in end-stage renal disease patients on dialysis: a case-control study
Publication date: Available online 20 July 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): G. Gandhi, G. Tung Patients with end-stage renal disease (ESRD) require hemodialysis. However, dialysis therapy may cause genomic damage due to increased oxidative stress. Non-invasive assessment of genotoxicity may be helpful for developing management strategies. We applied the buccal micronucleus cytome (BMCyt) assay to ESRD patients on dialysis. Patients (n=35, age 52±2 year) on dialysis therapy (20.9±0.8months) had low glomerular filtration rates (GFR=5.00±0.36ml/min...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 21, 2017 Category: Genetics & Stem Cells Source Type: research

Genotoxicity of gemfibrozil in the gilthead seabream (Sparus aurata)
We examined the genotoxicity of gemfibrozil in a fish species, the gilthead seabream (Sparus aurata), which is commercially important in southern Europe. Following 96-h waterborne exposure, molecular (erythrocyte DNA strand breaks) and cytogenetic (micronuclei and other nuclear abnormalities in cells) endpoints were measured. Gemfibrozil was positive in both endpoints, at environmentally relevant concentrations, a result that raises concerns about the potential genotoxic effects of the drug in recipient waters. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 18, 2017 Category: Genetics & Stem Cells Source Type: research

Personal samplers of bioavailable pesticides integrated with a hair follicle assay of DNA damage to assess environmental exposures and their associated risks in children
We report a practical and cost-effective approach to assess environmental pesticide exposures and their biological consequences in children. Our approach combines silicone wristband personal samplers and DNA damage quantification from hair follicles, and was tested as part of a community-based participatory research (CBPR) project involving ten Latino children from farmworker households in North Carolina. Our study documents high acceptance among Latino children and their caregivers of these noninvasive sampling methods The personal samplers detected organophosphates, organochlorines, and pyrethroids in the majority of the...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 16, 2017 Category: Genetics & Stem Cells Source Type: research

Developmental toxicity and DNA damaging properties of silver nanoparticles in the catfish (Clarias gariepinus)
Publication date: Available online 15 July 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Alaa El-Din H. Sayed, Hamdy A.M. Soliman Although, silver nanoparticles (AgNPs) are used in many different products, little information is known about their toxicity in tropical fish embryos. Therefore, this study evaluated the developmental toxicity of waterborne silver nanoparticles in embryos of Clarias gariepinus. Embryos were treated with (0, 25, 50, 75ng/L silver nanoparticles) in water up to 144h postfertilization stage (PFS). Results revealed various morphological malformations inclu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 15, 2017 Category: Genetics & Stem Cells Source Type: research

DNA damage and the bystander response in tumor and normal cells exposed to X-rays
Publication date: Available online 5 July 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): M. Subhashree, R. Venkateswarlu, K. Karthik, V. Shangamithra, P. Venkatachalam Monolayer and suspension cultures of tumor (BMG-1, CCRF-CEM), normal (AG1522, HADF, lymphocytes) and ATM-mutant (GM4405) human cells were exposed to X-rays at doses used in radiotherapy (high dose and high dose-rate) or radiological imaging (low dose and low dose-rate). Radiation-induced DNA damage, its persistence, and possible bystander effects were evaluated, based on DNA damage markers (γ-H2AX, p53ser15) ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 6, 2017 Category: Genetics & Stem Cells Source Type: research

Exposure to meat-derived carcinogens and bulky DNA adduct levels in normal-appearing colon mucosa
CONCLUSION Exposure to meat-derived carcinogens may be associated with increased DNA damage occurring directly in the colon among genetically susceptible individuals. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 4, 2017 Category: Genetics & Stem Cells Source Type: research

Genome-wide bisulfite sensitivity profiling of yeast suggests bisulfite inhibits transcription
Publication date: Available online 3 July 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Romulo Segovia, Veena Mathew, Annie Tam, Peter C. Stirling Bisulfite, in the form of sodium bisulfite or metabisulfite, is used commercially as a food preservative. Bisulfite is used in the laboratory as a single-stranded DNA mutagen in epigenomic analyses of DNA methylation. Recently it has also been used on whole yeast cells to induce mutations in exposed single-stranded regions in vivo. To understand the effects of bisulfite on live cells we conducted a genome-wide screen for bisulfite sen...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - July 3, 2017 Category: Genetics & Stem Cells Source Type: research

Mechanisms of chromosomal aberrations induced by sesamin metabolites in Chinese hamster lung cells
Publication date: Available online 30 June 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Yoshiko Ono, Namino Tomimori, Hisako Hori, Yoshinori Kitagawa, Hiroshi Shibata Sesamin is a major lignan in sesame seeds and oil. We previously demonstrated that sesamin induces chromosomal aberrations (CA) in Chinese hamster lung (CHL/IU) cells in the presence of a metabolic activation system (S9 mix), although no genotoxicity was detected in vivo. To clarify the mechanism of CA induction by sesamin, we identified its principal active metabolite. A mono-catechol derivative, [2-(3,4-methylen...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 30, 2017 Category: Genetics & Stem Cells Source Type: research

Apigenin prevents ultraviolet-B radiation induced cyclobutane pyrimidine dimers formation in human dermal fibroblasts
Publication date: Available online 27 June 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): S. Mary Britto, D. Shanthakumari, B. Agilan, T. Radhiga, G. Kanimozhi, N. Rajendra Prasad Exposure to solar ultraviolet-B (UVB) radiation leads to the formation of cyclobutane pyrimidine dimers (CPDs). We investigated the protective effect of apigenin against UVB-induced CPDs formation in human dermal fibroblasts cells (HDFa). For this purpose, HDFa cells were treated with apigenin (15μM) prior to UVB irradiation (20mJ/cm2); DNA damage and subsequent molecular end points were observed. Ex...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 27, 2017 Category: Genetics & Stem Cells Source Type: research

Ultrafine particles (UFPs) from domestic wood stoves: genotoxicity in human lung carcinoma A549 cells
Publication date: August 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 820 Author(s): Marabini Laura, Ozgen Senem, Turacchi Silvia, Aminti Stefania, Arnaboldi Francesca, Lonati Giovanni, Fermo Paola, Corbella Lorenza, Valli Gianluigi, Bernardoni Vera, Dell’Acqua Manuela, Vecchi Roberta, Becagli Silvia, Caruso Donatella, Corrado L. Galli, Marinovich Marina In this paper, results on the potential toxicity of ultrafine particles (UFPs d<100nm) emitted by the combustion of logwood and pellet (hardwood and softwood) are reported. The data were collected during the TOBICUP (...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 27, 2017 Category: Genetics & Stem Cells Source Type: research

Mutation spectrum resulting in M13mp2 phage DNA exposed to N-nitrosoproline with UVA irradiation
Publication date: Available online 23 June 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Yumi Horai, Yoshiko Ando, Sachiko Kimura, Sakae Arimoto-Kobayashi N-nitrosoproline (NPRO) is endogenously formed from proline and nitrite. In an effort to delineate the mechanism of NPRO-induced photomutagenicity, we investigated the mutagenic spectrum of NPRO on M13mp2 DNA with UVA irradiation. Following exposure to NPRO and UVA, the mutation frequency increased significantly in an NPRO and UVA dose-dependent manner. The sequence data derived from seventy of the mutants indicated that mutag...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 23, 2017 Category: Genetics & Stem Cells Source Type: research

Genotoxic effect of ultrafine particles (UFPs) from two domestic woody biomass stoves in human lung carcinoma cells (A549 cells)
Publication date: Available online 16 June 2017 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Marabini Laura, Ozgen Senem, Turacchi Silvia, Aminti Stefania, Arnaboldi Francesca, Lonati Giovanni, Fermo Paola, Corbella Lorenza, Valli Gianluigi, Bernardoni Vera, Dell’Acqua Manuela, Vecchi Roberta, Becagli Silvia, Caruso Donatella, Corrado L. Galli, Marinovich Marina In this paper, results on the potential toxicity of ultrafine particles (UFPs d <100nm) emitted by the combustion of logwood and pellet (hardwood and softwood) are reported. The data were collected during the TO...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 17, 2017 Category: Genetics & Stem Cells Source Type: research

Human pharmaceutical gemfibrozil induces genotoxicity in gilthead seabream Sparus aurata
In conclusion, the results raise concern about the potential genotoxic effects of the concentrations of gemfibrozil present in recipient waters. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - June 5, 2017 Category: Genetics & Stem Cells Source Type: research