Editorial Board
Publication date: January 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 837Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 28, 2018 Category: Genetics & Stem Cells Source Type: research

Scots pine as a promising indicator organism for biomonitoring of the polluted environment: A case study on chronically irradiated populations
Publication date: Available online 26 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Stanislav Geras’kin, Polina Volkova, Denis Vasiliyev, Nina Dikareva, Alla Oudalova, Elizaveta Kazakova, Ekaterina Makarenko, Gustavo Duarte, Alexey KuzmenkovAbstractIn this paper the main results of long-term (2003-2016) observations on Scots pine populations inhabiting sites affected by the Chernobyl accident are presented. Populations growing for many years under chronic radiation exposure are characterized by the enhanced mutation rates, increased genetic diversity, changes in the...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 27, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of the DNA damage in lymphocytes, sperm and buccal cells of workers under environmental and occupational boron exposure conditions
This study was conducted in two districts of Balıkesir; Bandırma and Bigadic, which geographically belong to the Marmara Region of Turkey. Bandırma is the production and exportation zone for the produced boric acid and some borates and Bigadic has the largest B deposits in Turkey. 102 male workers who were occupationally exposed to boron from Bandırma and 110 workers who were occupationally and environmentally exposed to boron from Bigadic participated to our study. In this study the DNA damage in the sperm, blood and buccal cells of 212 males was evaluated by comet and micronucleus assays. No significant increase in t...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 24, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of DNA damage in Polish children environmentally exposed to pesticides
In this study 108 children, living in areas of an intense pesticide use and a control group (n = 92) of children from an agrotouristic area were investigated, whether DNA damage increased due to prolonged pesticide exposure. A presence of DNA breaks and oxidative damage to DNA bases, characterized as Fpg-sensitive sites, were detected by comet assay. Micronuclei (MN) formation was evaluated by cytokinesis-block MN assay. The exposure of children to pesticides resulted in increased number of MN in peripheral blood lymphocytes (P = 0.016), increased DNA strand breaks level (P = 0.002...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 22, 2018 Category: Genetics & Stem Cells Source Type: research

Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
Publication date: Available online 21 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Dijana Topalović, Dragana Dekanski, Biljana Spremo-Potparević, Andrea Pirković, Sunčica Borozan, Vladan Bajić, Danilo Stojanović, Francesca Giampieri, Massimiliano Gasparrini, Lada ŽivkovićAbstractPhenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 22, 2018 Category: Genetics & Stem Cells Source Type: research

18F-FDG PET/CT scanning: biological effects on patients: entrance surface dose,: DNA damage, and chromosome aberrations in lymphocytes
Publication date: Available online 19 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Akshaya Prasad, Shangamithra Visweswaran, Karthik Kanagaraj, Venkateswarlu Raavi, M Arunan, E Venkatachalapathy, S Paneerselvam, MT Jose, Annalakshmi Ozhimuthu, Venkatachalam PerumalAbstractPositron Emission Tomography/Computed Tomography (PET/CT), a combination of PET and CT, is used in tumor staging, therapy planning, and treatment response monitoring. During PET imaging, patients receive low doses of radiation, which can induce an adaptive response and necessitate higher doses for thera...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 20, 2018 Category: Genetics & Stem Cells Source Type: research

Manganese oxide nanoparticles induce genotoxicity and DNA hypomethylation in the moss Physcomitrella patens
Publication date: Available online 18 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ilika Ghosh, Abhishek Sadhu, Yuji Moriyasu, Maumita Bandyopadhyay, Anita MukherjeeGraphical abstract (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 19, 2018 Category: Genetics & Stem Cells Source Type: research

Buccal epithelial cell micronuclei: sensitive, non-invasive biomarkers of occupational exposure to low doses of ionizing radiation
Publication date: Available online 19 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Laura Aguiar Torres, Armindo dos Santos Rodrigues, Diana Linhares, Ricardo Camarinho, Zélia Maria Nunes Páscoa Soares Rego, Patrícia Ventura GarciaAbstractWe have evaluated buccal epithelial cell micronucleus formation as a biomarker of genotoxic damage following occupational exposure to low doses of ionizing radiation. Buccal epithelial cells were collected from 42 occupationally exposed medical personnel and 39 non-exposed individuals (reference group). Each individu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 19, 2018 Category: Genetics & Stem Cells Source Type: research

Micronuclei frequency in peripheral blood lymphocytes and levels of anti-p53 autoantibodies in serum of residents of Kowary city regions (Poland) with elevated indoor concentrations of radon
ConclusionThe problem of the radon exposure is still unresolved and needs further studies on bigger human cohorts in order to search for more sensitive biomarkers. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2018 Category: Genetics & Stem Cells Source Type: research

Lactobacillus plantarum MON03 Counteracts Zearalenone Génotoxicty in Mice; chromosome aberrations, Micronuclei, DNA fragmentation and Apoptotique gene expression
This study was conducted to evaluate the ability of lactic acid bacteria, isolated from Tunisia traditional butter, Lactobacillus plantarum MON03 (LP) to protect mice against cytotoxicity and genotoxicity induced by ZEN. Two doses of LP (2 × 109 CFU/L, ~ 2 mg/kg and 4 × 109 CFU/L, ~ 4 mg/kg) was added alone or in combination with a toxic intragastric ZEN (40 mg/kg representing 8% of LD50) dose daily for 2 wk by oral gavage. The control group received distilled water. The positive control groups received Colchicin (4 mg/kg bw) for the micronucleus assay and...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2018 Category: Genetics & Stem Cells Source Type: research

Ambient fine particulate matter (PM2.5) induces oxidative stress and pro-inflammatory response via up-regulating the expression of CYP1A1/1B1 in human bronchial epithelial cells in vitro
Publication date: Available online 12 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Qi Yuan, Yaoyao Chen, Xiaobo Li, Haiyan Chu, Zhengdong ZhangAbstractWe investigated the mechanism responsible for the oxidative stress and pro-inflammatory response triggered by PM2.5 collected from Nanjing of China. Two human bronchial epithelia cell lines (HBE and BEAS-2B) were used. Human gene expression profile microarray was performed to investigate the alteration of gene expression in PM2.5-treated HBE cells. The results of ROS assay and ELISA indicated that PM2.5 (150 µ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 13, 2018 Category: Genetics & Stem Cells Source Type: research

Early prediction of liver carcinogenicity due to occupational exposure to pesticides
This study aimed to estimate novel biomarkers for early prediction of liver malignancy due to occupational exposure to pesticides in two groups of workers with different socioeconomic standard (highly educated urban researchers and low educated rural pesticides sprayers). This study included 50 urban researchers and 50 rural pesticides sprayers occupationally exposed to pesticides. They were compared with 50 non-exposed urban researchers and 50 non-exposed rural subjects. Several tumor biomarkers were estimated; P53 protein, Alfa fetoprotein (AFP), and Alpha-L-fucosidase (AFU). Additionally, telomerase enzyme activity, Rel...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 12, 2018 Category: Genetics & Stem Cells Source Type: research

Optimization of the harvesting and freezing conditions of human cell lines for DNA damage analysis by the alkaline Comet assay
This study was conducted in glial A172 and lung alveolar epithelial A549 cells. Two cell detachment methods (mechanical vs enzymatic) and two cryoprotective media [FBS + 10% DMSO vs Cell Culture Media (CCM) + 10% DMSO] were tested, and DNA damage assessed at four time points following storage at - 80 °C (one, two, four and eight weeks). In both cell lines, no differences in % tail intensity were detected between fresh and frozen cells up to eight weeks, irrespective of the harvesting method and freezing medium used. However, freshly isolated A172 cells exhibited a significant lower DNA damage when ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 11, 2018 Category: Genetics & Stem Cells Source Type: research

Use of human derived liver cells for the detection of genotoxins in comet assays
Publication date: Available online 10 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Miroslav Mišík, Armen Nersesyan, Nathalie Ropek, Wolfgang W Huber, Elisabeth Haslinger, Siegfried KnasmuellerAbstractOne of the problems of in vitro genotoxicity testing is the inadequate representation of drug metabolizing enzymes in indicator cells which are currently used. An alternative is the use of human derived liver cells lines which retained the activities of enzymes that catalyze the activation and detoxification of genotoxins. Several cell lines were identified whi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 11, 2018 Category: Genetics & Stem Cells Source Type: research

Nephrotoxicity: topical issue
Publication date: Available online 6 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Alena Gabelova, Katarina Kozics, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Monika SramkovaAbstractDrug-induced kidney injury is one of the most significant adverse events and dose limiting factor in chemotherapy as well a major cause of prospective drug attrition during pharmaceutical development. Moreover, kidney injury can also occur as a consequence of exposures to environmental xenobiotics such as heavy metals, fungal toxins and nanomaterials. The lack of adequate in vitro human kidne...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 7, 2018 Category: Genetics & Stem Cells Source Type: research

Effect of inter-individual variability in human liver cytochrome P450 isozymes on cyclophosphamide-induced micronucleus formation
Publication date: Available online 5 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Yuki Kishino, Tomoko Hasegawa, Ayako Kato, Yumi Nishiya, Veronika Rozhnal, Kyoko Watanabe, Wataru Takasaki, Takashi Yamoto, Kazuhiko MoriAbstractWe investigated the relationship between metabolic activities of cytochrome P450 (CYP) isozymes present in microsomal fractions derived from the livers of 78 donors and micronucleus induction by cyclophosphamide (CPA). Consequently, a wide inter-individual variation in CYP activities was observed among the 78 donors. The CYP activities were partial...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 6, 2018 Category: Genetics & Stem Cells Source Type: research

“Risk is in the air”: Polycyclic aromatic hydrocarbons, metals and mutagenicity of atmospheric particulate matter in a town of Northern Italy (Respira study)
Publication date: Available online 16 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Donatella Feretti, Roberta Pedrazzani, Elisabetta Ceretti, Mario Dal Grande, Ilaria Zerbini, Gaia Claudia Viviana Viola, Umberto Gelatti, Francesco Donato, Claudia ZaniAbstractAir pollution has well-known harmful effects on human beings, causing both acute and chronic diseases. Some data suggest that genetic damage occurring early in life may influence the risk of having cancer and other chronic diseases in adulthood. Therefore, there is a growing interest in studying the genotoxic activit...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 5, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of oxidative damage induced by iron oxide nanoparticles on different nervous system cells
Publication date: Available online 30 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Natalia Fernández-Bertólez, Carla Costa, Maria João Bessa, Margriet Park, Marie Carriere, Fanny Dussert, João Paulo Teixeira, Eduardo Pásaro, Blanca Laffon, Vanessa ValdiglesiasAbstractIron oxide nanoparticles (ION) have received much attention for their utility in biomedical applications, such as magnetic resonance imaging, drug delivery and hyperthermia, but concerns regarding their potential harmful effects are also growing. Even though ION may induce ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 1, 2018 Category: Genetics & Stem Cells Source Type: research

Comet assay in neural cells as a tool to monitor DNA damage induced by chemical or physical factors relevant to environmental and occupational exposure
Publication date: Available online 30 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Marcin Kruszewski, Katarzyna Sikorska, Sylwia Meczynska-Wielgosz, Agnieszka Grzelak, Monika Sramkova, Alena Gabelova, Lucyna Kapka-SkrzypczakAbstractBiomonitoring of the effects of environmental and occupational exposure relevant chemical or physical factors on central nervous system is difficult due to the problems with sampling of biological material. Thus, surrogate systems allowing for the estimation of effect intensity are necessary to evaluate a potential risk of exposure. Cancerous ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 1, 2018 Category: Genetics & Stem Cells Source Type: research

Genotoxicity evaluation of titanium dioxide nanoparticles using the mouse lymphoma assay and the Ames test
Publication date: Available online 1 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Xiuming Du, Shunxiang Gao, Liling Hong, Xin Zheng, Qingyun Zhou, Jihong WuAbstractTitanium dioxide nanoparticles (TiO2-NPs) are widely used in the cosmetics, health, and food industries, but their safety and genotoxicity remain a matter of debate. We investigated whether TiO2-NPs could induce gene mutations in mouse lymphoma L5178Y cells and Salmonella typhimurium strains TA97a, TA98, TA100, TA102, and TA1535. Following preliminary tests, 2 mg/mL for the mouse lymphoma gene mutation ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 1, 2018 Category: Genetics & Stem Cells Source Type: research

Genotoxic and oxidative damage in the freshwater teleost Prochilodus lineatus exposed to the insecticides lambda-cyhalothrin and imidacloprid alone and in combination
The objective of this study was to evaluate genotoxic and oxidative effects of LC and IMI, isolated and in binary mixture, on the neotropical freshwater teleost curimba (Prochilodus lineatus). Juveniles of P. lineatus were exposed to LC (0.15 μg L-1), IMI (100 μg L-1), LC + IMI or only to water (CTR) for 48, 96, 144 and 192 h. The occurrence of DNA damage (comet assay), was evaluated in erythrocytes, gills, liver and brain cells, the frequency of micronucleus (MN) and nuclear abnormalities (ENA) was measured in erythrocytes and protein carbonylation (PCC) and lipid per...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 29, 2018 Category: Genetics & Stem Cells Source Type: research

Effect of age and sex on the level of DNA strand breaks and oxidatively damaged DNA in human blood cells
Publication date: Available online 27 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Peter MøllerAbstractDNA damage measured by the comet assay is a well-established biomarker in studies on environmental and occupational exposures, dietary factors and clinical outcomes. Age and sex are typically regarded as confounding factors that are controlled by either selective inclusion criteria of subjects or adjustment in the statistical analysis. This review assesses the influence of age and sex on levels of DNA damage in leukocytes in study populations of healthy subjects....
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 28, 2018 Category: Genetics & Stem Cells Source Type: research

Cytotoxic and genotoxic effects of environmental relevant concentrations of bisphenol A and interactions with doxorubicin
This study is intended to assess potential cytotoxic and genotoxic effects of BPA, as well as its interactions with DOX, in Human epithelial type 2 cells (Hep-2) originated from a human laryngeal carcinoma and in a DNA damage responsive cell line, the human lung fibroblasts (MRC-5). Cell viability was analyzed through the resazurin assay. The G protein-coupled estrogen receptor 1 (GPER) expression was visualized by immunodetection. Genotoxicity, namely DNA damage and oxidative DNA damage, were assessed by comet assay and micronuclei induction, and mitotic disruption was evaluated cytologically by fluorescent microscopy wit...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 28, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of 12 mouse marker genes in rat toxicogenomics public data, Open TG-GATEs: discrimination of genotoxic from non-genotoxic hepatocarcinogens
Publication date: Available online 23 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Chie Furihata, Takayoshi SuzukiAbstractPreviously, we proposed 12 marker genes (Aen, Bax, Btg2, Ccnf, Ccng1, Cdkn1a, Gdf15, Lrp1, Mbd1, Phlda3, Plk2 and Tubb4b) to discriminate mouse genotoxic hepatocarcinogens (GTHC) from non-genotoxic hepatocarcinogens (NGTHC). This was determined by qPCR and principal component analysis (PCA), as the aim of an in vivo short-term screening for genotoxic hepatocarcinogens. For this paper, we conducted an application study of the 12 mouse marker genes to r...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 24, 2018 Category: Genetics & Stem Cells Source Type: research

Blood levels of nitric oxide and dna breaks assayed in whole blood and isolated peripheral blood mononucleated cells in patients with multiple sclerosis
Publication date: Available online 23 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Vitālijs Borisovs, Elīna Ļeonova, Larisa Baumane, Jolanta Kalniņa, Natalja Mjagkova, Nikolajs SjaksteAbstractOxidative stress, especially overproduction of nitric oxide (NO), is considered to be one of the crucial factors in the pathogenesis of multifactorial multiple sclerosis (MS). DNA breaks could be one of the consequences of oxidative stress; however, data on DNA breakage in MS are very few and contradictory. There are no data on direct measurements of NO production in the blood o...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 24, 2018 Category: Genetics & Stem Cells Source Type: research

The enzyme-modified comet assay: enzyme incubation step in 2 vs 12-gels/slide systems
Publication date: Available online 22 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Damian Muruzabal, Sabine Langie, Bertrand Pourrut, Amaya AzquetaAbstractThe enzyme-modified comet assay is a commonly used method to detect specific DNA lesions. However, still a lot of errors are made by many users, leading to dubious results and even misinterpretations. This technical note describes some critical points in the use of the enzyme-modified comet assay, such as the enzyme concentration, the time of incubation, the format used and the equipment. To illustrate the importance o...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 22, 2018 Category: Genetics & Stem Cells Source Type: research

Toxicological impact of acute exposure to E171 food additive and TiO2 nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cells
Publication date: Available online 22 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Marie Dorier, Céline Tisseyre, Fanny Dussert, David Béal, Marie-Edith Arnal, Thierry Douki, Vanessa Valdiglesias, Blanca Laffon, Sónia Fraga, Fátima Brandão, Nathalie Herlin-Boime, Frédérick Barreau, Thierry Rabilloud, Marie CarriereAbstractTiO2 particles are widely used in products for everyday consumption, such as cosmetics and food; their possible adverse effects on human health must therefore be investigated. The aim of this study was to...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 22, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of dna damage in welders using comet and micronucleus assays
Publication date: Available online 19 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): İldeniz Aksu, Hatice Gül Anlar, Gökçe Taner, Merve Bacanlı, Servet İritaş, Engin Tutkun, Nursen BasaranAbstractWelding technology is widely used in pressurized containers, thermal power plants, refineries, chemical facilities and steel structures. Welders are exposed to a number of hazardous compounds such as ultraviolet (UV) radiation, electromagnetic fields, toxic metals and polycyclic aromatic hydrocarbons (PAHs). In the present study, 48 welders and an equal number...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 21, 2018 Category: Genetics & Stem Cells Source Type: research

Fish and salad consumption are inversely associated with levels of oxidatively damaged DNA in a Danish adult cohort
This study investigated associations between levels of oxidatively damaged DNA, measured by the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay and intake of fish, salad, fruits, vegetables, wholegrain items, and potatoes in a cross-sectional study of 382 men and 591 women between 18 and 93 years. Intake of dietary items was obtained from questionnaires, and stratified into less than once per week, weekly or daily consumption. Intake of fish as main course was inversely associated with levels of Fpg-sensitive sites in peripheral blood mononuclear cells (PBMCs) in especially women (P 
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 21, 2018 Category: Genetics & Stem Cells Source Type: research

Markedly enhanced micronucleus induction by 1,2-dimethylhydrazine dihydrochloride in colonic cells of rats with bacterial colonization in the intestine
Publication date: Available online 20 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Wakako OhyamaAbstractTo investigate how intestinal bacteria affect host cytogenetic alterations in the early initiation step of colon carcinogenesis, we conducted a comet assay and micronucleus (MN) test using germ-free (GF) and conventionalized (Cvd) rats after a single subcutaneous injection of the carcinogen, 1,2-dimethylhydrazine dihydrochloride (DMH). DNA damage was also determined in the liver in comet assays, as DMH is metabolized and activated in this organ. The time-response patte...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 21, 2018 Category: Genetics & Stem Cells Source Type: research

Risk Is In The Air”: Polycyclic Aromatic Hydrocarbons, Metals And Mutagenicity Of Atmospheric Particulate Matter In A Town Of Northern Italy (Respira Study)
Publication date: Available online 16 November 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Donatella Feretti, Roberta Pedrazzani, Elisabetta Ceretti, Mario Dal Grande, Ilaria Zerbini, Gaia Claudia Viviana Viola, Umberto Gelatti, Francesco Donato, Claudia ZaniAbstractAir pollution has well-known harmful effects on human beings, causing both acute and chronic diseases. Some data suggest that genetic damage occurring early in life may influence the risk of having cancer and other chronic diseases in adulthood. Therefore, there is a growing interest in studying the genotoxic activit...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 17, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: December 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 836, Part BAuthor(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 14, 2018 Category: Genetics & Stem Cells Source Type: research

A systematic review of the use of the alkaline comet assay for genotoxicity studies in human colon-derived cells
Publication date: Available online 30 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ezgi Eyluel Bankoglu, Geema Kodandaraman, Helga StopperAbstractThis review describes the use of the comet assay for assessment of DNA damage in human colon cells. We screened 98 papers, which employed human colon -derived cells to analyse DNA damage induced by different insults with the comet assay. In most cases tumour cell lines were used, and only a few studies were performed with primary colon cells. The comet assay was mostly applied to test chemotherapeutics and natural products. We c...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - November 4, 2018 Category: Genetics & Stem Cells Source Type: research

A systematic review of the use of the comet assay for genotoxicity studies in human colon-derived cells
Publication date: Available online 30 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ezgi Eyluel Bankoglu, Geema Kodandaraman, Helga StopperAbstractThis review describes the use of the comet assay for assessment of DNA damage in human colon cells. We screened 98 papers, which employed human colon -derived cells to analyse DNA damage induced by different insults with the comet assay. In most cases tumour cell lines were used, and only a few studies were performed with primary colon cells. The comet assay was mostly applied to test chemotherapeutics and natural products. We c...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 31, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: December 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 836, Part AAuthor(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 31, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) mutagenicity using in vitro and in vivo Pig-a assays
Publication date: Available online 29 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Roberta A. Mittelstaedt, Vasily N. Dobrovolsky, Javier R. Revollo, Mason G. Pearce, Yiying Wang, Azra Dad, Page B. McKinzie, Hans Rosenfeldt, Berran Yucesoy, Raymond Yeager, Shu-Chieh Hu, Yunan Tang, Seonggi Min, Hyun-Ki Kang, Dong-Jin Yang, Mallikarjuna Basavarajappa, Robert H. HeflichAbstract4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a genotoxic carcinogen found in tobacco and tobacco smoke. Several in vitro and in vivo assays have been used for evaluating the genotoxicity of...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 30, 2018 Category: Genetics & Stem Cells Source Type: research

Cytoprotective and genotoxic effects of vitamins K1 and B1 on irinotecan in vitro
Publication date: Available online 26 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Eleni Giarma, Zoi Mpampali, Th. Lialiaris, D. MourelatosAbstractCultured human lymphocytes were treated with vitamins K1 and B1, potential anticancer agents, either alone or in combination with irinotecan, a semisynthetic analogue of camptothecin. The frequency of sister chromatid exchanges (SCEs) was measured as an indicator of genotoxicity and the proliferation rate index (PRI) and mitotic index (MI) was measured as indicators of cytostatic effect. Vitamin K1 alone did not induce SCEs at ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 27, 2018 Category: Genetics & Stem Cells Source Type: research

Application of the comet assay, micronucleus test and global DNA methylation analysis in Darevskia lizards as a sentinel organism for genotoxic monitoring of soil pollution
In conclusion, the current study suggests that DNA damage and global DNA methylation may be applied to Darevskia lizards as a sentinel organism for assessing the effects of environmental pollutants. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 26, 2018 Category: Genetics & Stem Cells Source Type: research

Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay
Publication date: Available online 16 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Gracia Montag, Ezgi Eyluel Bankoglu, Annika Bolte, Henning Hintzsche, Ninoslav Djelic, Helga StopperAbstractThe aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation.A 4-hour MMS treatment induced a significant and...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 17, 2018 Category: Genetics & Stem Cells Source Type: research

Genotoxicity of textile dye C.I. Disperse Blue 291 in mouse bone marrow
Publication date: Available online 12 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Fábio Henrique Fernandes, Gisela de Aragão Umbuzeiro, Daisy Maria Fávero SalvadoriAbstractColor Index (C.I.) Disperse Blue 291 (DB291) is an azo dye used by the textile industry. After yarn dyeing, wastewater containing the dye, released into the aquatic environment, may pollute drinking water sources. We investigated the mutagenicity and genotoxicity of DB291 in male Swiss mice, following oral administration. Micronucleated cells, primary DNA damage (comet assay) in bl...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 13, 2018 Category: Genetics & Stem Cells Source Type: research

Increased genomic damage and vitamin B status in inflammatory bowel disease patients: A case-control, prospective, pilot study
This study investigated the frequency of micronuclei (MNi) using the CBMN-Cyt assay and the level of vitamin B in patients with IBD. This prospective study was conducted in 15 patients with ulcerative colitis, 15 patients with Crohn’s disease, and 30 healthy controls from one tertiary hospital. Serum vitamin B and homocysteine levels were measured, and the MNi status was analyzed using the CBMN-Cyt assay. The patients with IBD showed significantly lower serum pyridoxine levels and significantly higher homocysteine levels than controls. The frequencies of binucleated cells (BNCs) with MNi, nucleoplasmic bridges (NPBs)...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 12, 2018 Category: Genetics & Stem Cells Source Type: research

Cytogenetic status of interventional radiology unit workers occupationally exposed to low-dose ionising radiation: a pilot study
Publication date: Available online 11 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Marko Gerić, Jelena Popić, Goran Gajski, Vera Garaj-VrhovacAbstractInterventional radiology unit workers represent one of the occupationally most exposed populations to low-dose ionizing radiation. Since there are many uncertainties in research of doses below 100 mSv, this study attempted to evaluate DNA damage levels in chronically exposed personnel. The study group consisted of 24 subjects matched with a control population by the number of participants, age, gender ratio, active smoking...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 12, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: November 2018Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 835Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

A meta-analysis of epidemiologic studies of occupationally exposed styrene workers and micronuclei levels
Publication date: January 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 837Author(s): James J. CollinsAbstractPrevious reviews and meta-analyses have reached different conclusions on whether high styrene exposure increase micronucleus frequencies. The most recent meta-analysis reports an increase in these frequencies related to styrene. We update this meta-analysis of micronucleus frequencies with additional studies not previously included, eliminate double counting of study subjects appearing in more than one publication, considered levels of styrene exposures in the analysis, asse...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

In vivo genotoxicity of 1,4-dioxane evaluated by liver and bone marrow micronucleus tests and Pig-a assay in rats
Publication date: January 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 837Author(s): Satoru Itoh, Chiharu HattoriAbstract1,4-Dioxane, used widely as a solvent in the manufacture of chemicals and as a laboratory reagent, induced liver adenomas and carcinomas in mice and rats, and nasal tumors in rats in several long-term studies. 1,4-Dioxane has been reported to be non-genotoxic in vitro, and there is no clear conclusion concerning its in vivo genotoxicity in rodents. In the present study, we investigated the ability of 1,4-dioxane to induce micronuclei in the liver and bone marrow ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes
Publication date: January 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 837Author(s): Mojtaba Kianmehr, Mahmood Reza Mottaghy Shahri, Mojtaba Afsharnia, Zahra Rohani, Mohammad GhorbaniAbstractChlorination has been used as a major disinfectant process for swimming pool water in many countries. The purpose of this study is to compare the DNA damage of the blood lymphocytes in indoor pool lifeguards with non-lifeguards athletes. We performed a study in which the participants were Gonabad’s lifeguards. We chose 30 participants (15 male and 15 female) for each group. We collected v...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

Environmental exposure of humans to bromide in the Dead Sea area: Measurement of genotoxicy and apoptosis biomarkers
Publication date: January 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 837Author(s): Shreen Deeb Nusair, Mohammad Jamil Almasaleekh, Hasan Abder-Rahman, Mohammad AlkhatatbehAbstractBromide (Br−) is a bromine atom with a negative charge which is released mainly in the production of pesticides and flame retardants. It is also found naturally in seawater. Br¯ has been associated with many detrimental effects such as respiratory problems, gastric hemorrhages, and dermal burns. The aim of the study was to monitor serum bromide in humans and to correlate its level with genotox...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - October 5, 2018 Category: Genetics & Stem Cells Source Type: research

Analysis of historical negative control group data from the in vitro micronucleus assay using human lymphocytes
Publication date: Available online 18 September 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): David P. Lovell, Mick Fellow, Azeddine Elhajouji, Christopher S. Farabaugh, Ben G. Gilby, Kiyohiro Hashimoto, Yan Li, Shambhu Roy, Maik Schuler, James Whitwell, Jennifer Y. TanirAbstractA database of the micronuclei counts was built up for historical negative control data from human lymphocyte in vitro micronuclei tests (MnVit) carried out in 8 laboratories with experience of the method. The mean incidence of micronucleated cells (mnt) /1000 cells ranged from 2.2/1000 to 15.9/1000. There ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 20, 2018 Category: Genetics & Stem Cells Source Type: research

Integration of micronucleus tests with a gene mutation assay in F344 gpt delta transgenic rats using benzo[a]pyrene
Publication date: Available online 16 September 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Hisako Hori, Satomi Shimoyoshi, Yasuhiro Tanaka, Ayaka Momonami, Kenichi Masumura, Masami Yamada, Wataru Fujii, Yoshinori KitagawaAbstractReduction of the number of animals used in in vivo genotoxicity tests is encouraged. For this purpose, we conducted integrated toxicity tests combining gene mutation assays with multiple-organ micronucleus (MN) tests (peripheral blood, bone marrow, liver, and colon) in F344 gpt delta transgenic (Tg) rats. Seven-week-old male F344 gpt delta rats were ora...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 17, 2018 Category: Genetics & Stem Cells Source Type: research

Preface to the Special Issue dedicated to the X Latin-American Congress of Environmental Mutagenesis, Carcinogenesis and Teratogenesis Association (X ALAMCTA Meeting)
Publication date: Available online 10 September 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): López Wilner (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - September 11, 2018 Category: Genetics & Stem Cells Source Type: research