Editorial Board
Publication date: February 2020Source: Molecular and Cellular Probes, Volume 49Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 18, 2020 Category: Molecular Biology Source Type: research
Development of a novel and rapid polymerase spiral reaction (PSR) assay to detect Salmonella in pork and pork products
Publication date: Available online 15 January 2020Source: Molecular and Cellular ProbesAuthor(s): Kasanchi M. Momin, Arockiasamy Arun Prince Milton, Sandeep Ghatak, Shiny C. Thomas, Govindarajan Bhuvana Priya, Samir Das, Ingudam Shakuntala, Rajkumari Sanjukta, Kekungu-u Puro, Arnab SenAbstractThe polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conventional end-poin...
Source: Molecular and Cellular Probes - January 15, 2020 Category: Molecular Biology Source Type: research
Evaluating two approaches for using positive control in standardizing the avian influenza H5 reverse transcription recombinase polymerase amplification assay
In conclusion, the two-tubes-per-one-sample approach was more reliable to control the false negative results in H5 RT-RPA assay. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 15, 2020 Category: Molecular Biology Source Type: research
LncRNA AFAP1-AS1 promotes osteoblast differentiation of human aortic valve interstitial cells through regulating miR-155/SMAD5 axis AFAP1-AS1 promotes osteoblast differentiation of VICs
ConclusionCollectively, AFAP1-AS1 promotes osteogenic differentiation of VICs, at least in part, by sponging miR-155 to upregulate SMAD5. This study sheds new light on lncRNA-directed therapeutics in DCAVD. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 14, 2020 Category: Molecular Biology Source Type: research
Epitope mapping of the White Spot Syndrome Virus (WSSV) VP28 monoclonal antibody through combined in silico and in vitro analysis reveals the potential antibody binding site
Publication date: Available online 11 January 2020Source: Molecular and Cellular ProbesAuthor(s): P.V. Shine, K.M. Shankar, B. Abhiman, N.S. Sudheer, R. PatilAbstractWhite Spot Syndrome Virus (WSSV) infecting shrimp is an enveloped double-stranded DNA virus. The WSSV is a member of the genus Whispovirus. The envelope protein VP28 is the most investigated protein of WSSV. In the present study, the epitope mapping of the monoclonal antibody (MAb) C-33 was carried out. Based on the epitope mapping results, an antigen-antibody interaction model was derived. Peptide scanning and confirmation of epitopes of MAb C-33 were carried...
Source: Molecular and Cellular Probes - January 12, 2020 Category: Molecular Biology Source Type: research
A novel simple genotyping assay for detection of the ‘Gait keeper’ mutation in DMRT3 and allele frequencies in Azteca and Costa Rican Saddle Horse breeds
Publication date: Available online 7 January 2020Source: Molecular and Cellular ProbesAuthor(s): Miguel Angel Ayala-Valdovinos, Jorge Galindo-García, David Sánchez-Chiprés, Theodor Duifhuis-Rivera, Rubén Anguiano-EstrellaAbstractThe ‘Gait keeper’ mutation in the DMRT3 gene alters locomotion and gait patterns in horses. This mutation (C>A) has been found in all gaited breeds of horses analyzed but is absent in most non-gaited breeds. We developed a new mutagenically separated polymerase chain reaction (MS-PCR) based method for simple detection of horse DMRT3 genotype. Our method was applied in a preliminary study to...
Source: Molecular and Cellular Probes - January 8, 2020 Category: Molecular Biology Source Type: research
Detection of cyprinid herpesvirus 2 by loop-mediated isothermal amplification in combination with a lateral flow dipstick
Publication date: Available online 3 January 2020Source: Molecular and Cellular ProbesAuthor(s): Yanli Li, Feng Lin, Lihui Sun, Aixia Huang, Jianming Chen, Guijie Hao, Xuemei Yuan, Haiqi Zhang, Shengqi SuAbstractWe developed a convenient technique to detect Herpesviral haematopoietic necrosis attributed to cyprinid herpes virus 2 (CyHV-2), a serious disease of Crucian carp and goldfish related to high mortality. In the present study, we employed a lateral flow dipstick (LAMP-LFD) to present a loop-mediated isothermal amplification assay. The specificity was ascertained via other six viruses, and the sensitivity was compare...
Source: Molecular and Cellular Probes - January 5, 2020 Category: Molecular Biology Source Type: research
Rapid and sensitive detection of potato virus Y by isothermal reverse transcription-recombinase polymerase amplification assay in potato
In this study, an isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed for the efficient and accurate detection of potato virus Y (PVY) under isothermal conditions. This RT-RPA assay was more efficient than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay as the amplification reaction can be completed in less than 20 min. Moreover, unlike PCR that requires a thermocycler to carry out the DNA amplification through specific temperature phases, RPA assay could be performed under an isothermal condition at a temperature ranging from 25 to 40 °C. ...
Source: Molecular and Cellular Probes - January 3, 2020 Category: Molecular Biology Source Type: research
Sp1 promotes dental pulp stem cell osteoblastic differentiation through regulating noggin
In conclusion, our study revealed that Sp1 regulates DPSCs osteoblastic differentiation through noggin and that Sp1/noggin can provide new perspective for enhancing DPSCs osteogenesis. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 3, 2020 Category: Molecular Biology Source Type: research
Lung resided monocytic myeloid-derived suppressor cells contribute to premetastatic niche formation by enhancing MMP-9 expression
Publication date: Available online 28 December 2019Source: Molecular and Cellular ProbesAuthor(s): Juechao Zhang, Xiaoqing Han, Huifang Shi, Yanyan Gao, Xuan Qiao, Huihan Li, Min Wei, Xianlu ZengAbstractIn cancer patients, the prevalence of myeloid-derived suppressor cells (MDSCs) is correlated with the degree of malignancy. In the present study, we investigated the role of circulating M-MDSCs in premetastatic niche formation using a mouse syngeneic tumor model and found that there was an increased frequency of M-MDSCs in the peripheral blood of tumor-bearing mice. M-MDSCs tracking and lung tissue histological analyses rev...
Source: Molecular and Cellular Probes - December 29, 2019 Category: Molecular Biology Source Type: research
Development of PCR based assays for detection of lethal Holstein haplotype 1, 3 and 4 in Holstein Friesian cattle
Publication date: Available online 27 December 2019Source: Molecular and Cellular ProbesAuthor(s): Anshuman Kumar, I.D. Gupta, Govind Mohan, M.R. Vineeth, D. Ravi kumar, Jayakumar S, S.K. NiranjanAbstractHolstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays – tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques for screening of HH1, 3 and 4, respectively were developed and validated. During screening, six among 60 H...
Source: Molecular and Cellular Probes - December 29, 2019 Category: Molecular Biology Source Type: research
lncRNA XIST attenuates hypoxia-induced H9c2 cardiomyocyte injury by targeting the miR-122-5p/FOXP2 axis
ConclusionlncRNA XIST could regulate the miR-122-5p/FOXP2 axis to attenuate hypoxia-induced H9c2 cardiomyocyte injury. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 29, 2019 Category: Molecular Biology Source Type: research
Multislice spiral CT images combined with CEA and lymphocyte-to-neutrophil ratio predict recurrence and post-operative metastasis of rectal cancer
Publication date: Available online 28 December 2019Source: Molecular and Cellular ProbesAuthor(s): Hui-yuan Deng, Xiang-qing Zhu, Ying-ying Ding, Jin-dan Li, Jun Yang, Teng-fei Ke, Rui Wang, Qiang Chen, Jing Hu, Yan-ying Wang, Cheng-de LiaoAbstractTo explore the early predictors of post-operative recurrence and metastasis of rectal cancer, analyse the associated risk, and construct a model. Retrospective collection. Four hundred patients with rectal cancer underwent surgical resection and pathological diagnosis from September 2013 to September 2014. During the post-operative period, the patients were tested by imaging exam...
Source: Molecular and Cellular Probes - December 29, 2019 Category: Molecular Biology Source Type: research
Rapid analysis of Escherichia coli O157:H7 using isothermal recombinase polymerase amplification combined with triple-labeled nucleotide probes
Publication date: Available online 27 December 2019Source: Molecular and Cellular ProbesAuthor(s): Jinqiang Hu, Yi Wang, Haijian Su, Huimin Ding, Xincheng Sun, Hui Gao, Yao Geng, Zhangcun WangAbstractRapid analytical methods are urgently needed to evaluate Escherichia coli (E. coli) O157:H7 in food. In this work, a novel recombinase polymerase amplification (RPA)-based lateral flow dipstick (LFD) method was developed to detect E. coli. Briefly, suitable primers and probes were designed and screened. Then, RPA reaction parameters, including volume, time, and temperature, were optimized. The specificity and sensitivity of RP...
Source: Molecular and Cellular Probes - December 27, 2019 Category: Molecular Biology Source Type: research
MiR-20a-5p suppressed TGF-β1-triggered apoptosis of human bronchial epithelial BEAS-2B cells by targeting STAT3
Publication date: Available online 25 December 2019Source: Molecular and Cellular ProbesAuthor(s): Xiuyun Lv, Lihong Wang, Tianji ZhuAbstractApoptosis of bronchial epithelial cells contributes to lung diseases, including asthma. Although miR-20a-5p is reportedly downregulated in the bronchial epithelia of asthmatic patients, its function and mechanism still need to be explored. Here, we explored how miR-20a-5p affects human bronchial epithelial cells stimulated with transforming growth factor (TGF)-β1. Using qRT-PCR, we observed downregulated miR-20a-5p levels in these cells. After transfecting miR-20a-5p mimics or inhibi...
Source: Molecular and Cellular Probes - December 26, 2019 Category: Molecular Biology Source Type: research
