Fibroblast growth factor-2 ameliorates tumor necrosis factor-alpha-induced osteogenic damage of human bone mesenchymal stem cells by improving oxidative phosphorylation
In this study, we investigated whether FGF2 can ameliorate TNF-a-inhibited osteogenic damage by improving OXPHOS. Effects of TNF-α or FGF2 on the proliferation and osteogenic differentiation of hBMSCs were evaluated by MTT assay, qRT-PCR, and ALP activity tests. The function of FGF2 on the TNF-a-inhibited metabolic switch was determined by Mito Stress test. The results showed that TNF-α was able to inhibit the osteogenic differentiation and OXPHOS of hBMSCs. FGF2 has no obvious function in improving the osteogenic-related genes, but it can ameliorate the impaired osteogenesis and OCR value caused by TNF-α. These finding...
Source: Molecular and Cellular Probes - February 20, 2020 Category: Molecular Biology Source Type: research
Overexpression of mir-129-1, miR-146b, mir-183, and mir-197 in follicular thyroid carcinoma and adenoma tissues
Publication date: Available online 18 February 2020Source: Molecular and Cellular ProbesAuthor(s): Sara Sheikholeslami, Noushin Shabani, Setareh Shivaee, Seyed Mohammad Tavangar, MarjanZarif Yeganeh, Mehdi Hedayati, Jabar Lotfi, Hanieh GholamiAbstractFollicular thyroid carcinoma (FTC) is responsible for approximately 10% of thyroid malignancies. Since this type of malignancy indicates no capsular and vascular invasions, adenoma and follicular carcinoma of thyroid are not distinguishable. It has been proved that microRNAs, which regulate approximately 30% of coding proteins, have an association with follicular thyroid adeno...
Source: Molecular and Cellular Probes - February 20, 2020 Category: Molecular Biology Source Type: research
The role of miR-21/RECK in the inhibition of osteosarcoma by curcumin
Publication date: Available online 17 February 2020Source: Molecular and Cellular ProbesAuthor(s): Li Zhou, Yang Lu, Jing-shu Liu, Shu-zi Long, Hong-liang Liu, Jie Zhang, Tao ZhangAbstractOsteosarcoma is a malignant tumor in bones that is common in children and adolescents. MicroRNAs (miRs) are small non-coding RNAs that are associated with various kinds of tumors. miR-21 is one of the most frequently overexpressed microRNAs in osteosarcoma. Curcumin is a naturally occurring phenolic compound that has antitumor properties. Curcumin significantly inhibits osteosarcoma. However, the role of miR-21 and its target gene, revers...
Source: Molecular and Cellular Probes - February 18, 2020 Category: Molecular Biology Source Type: research
Copy number variations in miscarriage products and their relationship with consanguinity and recurrent miscarriage in individuals with normal karyotypes
Publication date: Available online 16 February 2020Source: Molecular and Cellular ProbesAuthor(s): Parisa Atefvahid, Mohammad Hossein Modarressi, Ashraf Aleyasin, Zahra Noormohammadi (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - February 17, 2020 Category: Molecular Biology Source Type: research
Identification of Brucella spp. isolates and discrimination from the vaccine strain Rev.1 by MALDI-TOF mass spectrometry
In this study, a custom MALDI-TOF MS reference library was constructed and its performance on identification at species level was evaluated using 75 Brucella spp. isolates. Furthermore, distinct peak biomarkers were detected for biovar assignment and discrimination from vaccine strain Rev.1. Analysis of mass peak profiles allowed Brucella accurate identification at genus and species level (100%) with no misidentifications. Despite the high intrageneric similarity, MALDI-TOF MS database succeeded in classifying at biovar level, 47 out of 62 B. melitensis bv. 3 isolates (75.81%), whereas all B. melitensis strains, except for...
Source: Molecular and Cellular Probes - February 14, 2020 Category: Molecular Biology Source Type: research
Comparing the performance of conventional PCR, RTQ-PCR, and droplet digital PCR assays in detection of Shigella
Publication date: Available online 13 February 2020Source: Molecular and Cellular ProbesAuthor(s): Jin Yang, Nana Zhang, Jun Lv, Ping Zhu, Xing Pan, Jiaqingzi Hu, Wenfeng Wu, Shan Li, Hongtao LiAbstractThe incidence of foodborne infections caused by Shigella spp. is still very high in every year, which poses a great potential threat to public health. Conventional quantification methods based on culture techniques, biochemical, and serological identification are time-consuming and labor-intensive. To develop a more rapid and efficient detection method of Shigella spp., we compared the sensitivity and specificity of three di...
Source: Molecular and Cellular Probes - February 13, 2020 Category: Molecular Biology Source Type: research
A novel CNS-homing peptide for targeting neuroinflammatory lesions in experimental autoimmune encephalomyelitis
Publication date: Available online 5 February 2020Source: Molecular and Cellular ProbesAuthor(s): Bodhraj Acharya, Rakeshchandra R. Meka, Shivaprasad H. Venkatesha, Jason Lees, Tambet Teesalu, Kamal D. MoudgilAbstractUsing phage peptide library screening, we identified peptide-encoding phages that selectively home to the inflamed central nervous system (CNS) of mice with experimental autoimmune encephalomyelitis (EAE), a model of human multiple sclerosis (MS). A phage peptide display library encoding cyclic 9-amino-acid random peptides was first screened ex-vivo for binding to the CNS tissue of EAE mice, followed by in viv...
Source: Molecular and Cellular Probes - February 6, 2020 Category: Molecular Biology Source Type: research
Effects of hyperoxia exposure on the expression of Nrf2 and heme oxygenase-1 in lung tissues of premature rats
Publication date: Available online 6 February 2020Source: Molecular and Cellular ProbesAuthor(s): Xiaoyun Chu, Xiaoyue Zhang, Xiaohui Gong, Huilin Zhou, Cheng CaiAbstractBronchopulmonary dysplasia (BPD) is a chronic lung disease with long-term sequelae including neurodevelopmental delay. Although the precise mechanism of BPD is not well defined, oxidative stress is thought to be involved in the pathogenesis process of BPD. Nrf2 (Nuclear factor erythroid 2-related factor 2)-Keap1 (Kelch-like ECH associated protein 1)-ARE (Antioxidant Reaction Elements) signaling pathway is one of the main protective mechanisms of BPD, which...
Source: Molecular and Cellular Probes - February 6, 2020 Category: Molecular Biology Source Type: research
A highly sensitive semi-nested real-time PCR utilizing oligospermine-conjugated degenerate primers for the detection of diverse strains of small ruminant lentiviruses
Publication date: Available online 28 January 2020Source: Molecular and Cellular ProbesAuthor(s): Taxiarchis Chassalevris, Serafeim C. Chaintoutis, Evangelia D. Apostolidi, Nektarios D. Giadinis, Ioannis Vlemmas, Georgia D. Brellou, Chrysostomos I. DovasAbstractSmall ruminant lentiviruses (SRLVs) are highly diverse retroviruses infecting sheep and goats. Although PCR-based testing is being utilized for diagnostics, its application is hampered by various factors. These include, among others, the exceptionally high genetic variability of SRLVs, as well as the low number of infected blood monocytes. For this reason, a highly ...
Source: Molecular and Cellular Probes - January 29, 2020 Category: Molecular Biology Source Type: research
Exendin-4 regulates endoplasmic reticulum stress to protect endothelial progenitor cells from high-glucose damage
ConclusionExndin-4 can alleviate diabetes-caused damage to mice, moreover, it reduced endoplasmic reticulum stress and ROS through inhibiting p38 MAPK pathway in MPCs cells under high-glucose condition, thus increasing cell viability and reducing cell apoptosis. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 27, 2020 Category: Molecular Biology Source Type: research
Zoledronate inhibits the differentiation potential of adipose-derived stem cells into osteoblasts in repairing jaw necrosis
ConclusionThe differentiation of ADSCs into osteoblasts is significantly inhibited by ZOL. Due to this reason, it may be difficult to achieve good results by ZOL induced ADSCs into osteoblasts in repairing jaw necrosis. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 23, 2020 Category: Molecular Biology Source Type: research
Genetics of Parkinson's disease
This article aims to provide a comprehensive overview of the current knowledge in PD genetics. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 23, 2020 Category: Molecular Biology Source Type: research
Simultaneous detection of classical swine fever virus and porcine circovirus 3 by SYBR green I-based duplex real-time fluorescence quantitative PCR
In this study, 130 clinical samples collected from sick pigs in the field were tested by this assay with the positive rates of 9.23% (12/130) for CSFV and 21.54% (28/130) for PCV3 respectively, and the positive rate of CSFV and PCV3 co-infection was 6.92% (9/130). Our results showed that the developed method was a reliable diagnostic tool to monitor and survey CSFV, PCV3 and CSFV/PCV3 co-infection in the field. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 21, 2020 Category: Molecular Biology Source Type: research
Genome-wide analysis of mRNAs and lncRNAs in Mycoplasma bovis infected and non-infected bovine mammary gland tissues
Publication date: Available online 21 January 2020Source: Molecular and Cellular ProbesAuthor(s): Selçuk Özdemir, Serdar AltunAbstractMycoplasma bovis (M. bovis) causes diseases such as arthritis, pneumonia, abortion, and mastitis, leading to great losses in the bovine dairy industries. RNA types such as messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) play significant roles in regulating the immune responses triggered by bacteria. The expression profiles of mRNA and lncRNA as they occur in bovine mammary gland tissues infected with M. bovis are still not well understood. To illuminate this issue, transcription ...
Source: Molecular and Cellular Probes - January 21, 2020 Category: Molecular Biology Source Type: research
Exosomes derived from human bone marrow mesenchymal stem cells transfer miR-222-3p to suppress acute myeloid leukemia cell proliferation by targeting IRF2/INPP4B
ConclusionBM-MSCs delivered miR-222-3p via exosomes to inhibit cell proliferation and promote cell apoptosis by targeting IRF2 and negatively regulating IRF2/INPP4B signaling in THP-1 cells. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 20, 2020 Category: Molecular Biology Source Type: research
