Phosphatidylcholine absence affects the secretion of lipodepsipeptide phytoxins in Pseudomonas syringae pv. syringae van Hall CFCC 1336
In this study, we further analyzed virulence of lipodepsipeptide phytoxins of Pss 1336 wild type (pcs +), the 1336RM (pcs−/+) and the 1336 pcs− mutant, and found that the 1336 pcs− mutant was unable to cause necrosis of Chinese date fruits and inhibit fungal growth. HPLC analysis also showed that the 1336 pcs− mutant markedly reduced its secretion of lipodepsipeptide phytoxins. Analysis of semi-quantitative RT-PCR revealed that PC presence or absence did not affect gene expressions of SyrD, PseABC and PseEF efflux systems at transcriptional level. However, western blotting assays found that PseE and...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

Endophytism or saprophytism: Decoding the lifestyle transition of the generalist fungus Phomopsis liquidambari
Publication date: January 2018 Source:Microbiological Research, Volume 206 Author(s): Jun Zhou, Xin Li, Peng-Wei Huang, Chuan-Chao Dai Filamentous ascomycete Phomopsis sp. are common inhabitants of natural ecosystems and, as saprophytes, are largely responsible for the destructive decay of litterfall, promoting the carbon and nitrogen cycles. Phomopsis liquidambari B3 can establish mutualistic symbiosis with a broad spectrum of crop plants. Colonizing dynamics observations and a growth promotion assay of rice and Arabidopsis thaliana revealed that the B3 colonization strategy is host-adapted and resulted in different grow...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

SawR a new regulator controlling pyomelanin synthesis in Pseudomonas aeruginosa
In this study we describe how sawR regulates pyomelanin synthesis. We show that sawR down-regulates the expression levels of hmgA and this causes the accumulation of homogentisic acid which in turn undergoes oxidation and polymerization to pyomelanin. Using bioinformatics, we were able to identify a specific amino acid, arginine 23, which is found within the sawR DNA binding domain and is crucial for its regulatory activity. Our results indicate that sawR does not affect any other genes in the phenylalanine/tyrosine metabolic pathway and its repressive ability on hmgA is not mediated by the hmgA repressor PA2010 (i.e. hmgR...
Source: Microbiological Research - October 22, 2017 Category: Infectious Diseases Source Type: research

Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe
In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized...
Source: Microbiological Research - October 13, 2017 Category: Infectious Diseases Source Type: research

Pathogenic Labyrinthula associated with Australian seagrasses: Considerations for seagrass wasting disease in the southern hemisphere
Publication date: Available online 9 October 2017 Source:Microbiological Research Author(s): Stacey M. Trevathan-Tackett, Brooke K. Sullivan, Katie Robinson, Osu Lilje, Peter I. Macreadie, Frank H. Gleason Marine disease ecology is a growing field of research, particularly for host organisms negatively impacted by a changing climate and anthropogenic activities. A decrease in health and increase in susceptibility to disease has been hypothesised as the mechanism behind wide-spread seagrass die-offs related to wasting disease in the past. However, seagrass wasting disease and the causative pathogen, Labyrinthula, has been ...
Source: Microbiological Research - October 10, 2017 Category: Infectious Diseases Source Type: research

A comprehensive review of non-enterica subspecies of Salmonella enterica
Publication date: Available online 7 October 2017 Source:Microbiological Research Author(s): Alexandre Lamas, José Manuel Miranda, Patricia Regal, Beatriz Vázquez, Carlos Manuel Franco, Alberto Cepeda Salmonella is a major foodborne pathogen with a complex nomenclature. This genus is composed of two species, S. enterica and S. bongori. S. enterica is divided into six subspecies. S. enterica subspecies enterica is composed of more than 1,500 serotypes with some of great importance, such as S. Typhimurium and S. Enteritidis. S. enterica subsp. enterica is responsible of more than 99% of human salmonellosis and...
Source: Microbiological Research - October 8, 2017 Category: Infectious Diseases Source Type: research

Klebsiella sp. confers enhanced tolerance to salinity and plant growth promotion in oat seedlings (Avena sativa)
This study demonstrates that PGPR play an imperative function in stimulating salt tolerance in plants and can be used as biofertilizer to enhance growth of crops in saline areas. (Source: Microbiological Research)
Source: Microbiological Research - September 24, 2017 Category: Infectious Diseases Source Type: research

A novel Burkholderia ambifaria strain able to degrade the mycotoxin fusaric acid and to inhibit Fusarium spp. growth
Publication date: Available online 22 September 2017 Source:Microbiological Research Author(s): Ester Simonetti, Irma N. Roberts, Marcela S. Montecchia, Flavio H. Gutierrez-Boem, Federico M. Gomez, Jimena A. Ruiz Fusaric acid (FA) is a fungal metabolite produced by several Fusarium species responsible for wilts and root rot diseases of a great variety of plants. Bacillus spp. and Pseudomonas spp. have been considered as promising biocontrol agents against phytopathogenic Fusarium spp., however it has been demonstrated that FA negatively affects growth and production of some antibiotics in these bacteria. Thus, the capabil...
Source: Microbiological Research - September 23, 2017 Category: Infectious Diseases Source Type: research

Isolation and characterisation of theobromine-degrading filamentous fungi
Publication date: Available online 19 September 2017 Source:Microbiological Research Author(s): Daniel Oduro-Mensah, Augustine Ocloo, Sammy T. Lowor, Evelyn Y. Bonney, Laud K.N.A. Okine, Naa Ayikailey Adamafio Strategies for achieving global food security include identification of alternative feedstock for use as animal feed, to contribute towards efforts at increasing livestock farming. The presence of theobromine in cocoa pod husks, a major agro-waste in cocoa-producing countries, hinders its utilisation for this purpose. Cheap treatment of cocoa pod husks to remove theobromine would allow largescale beneficial use of t...
Source: Microbiological Research - September 20, 2017 Category: Infectious Diseases Source Type: research

Screening of Tropically Derived, Multi-Trait Plant Growth-Promoting Rhizobacteria and Evaluation of Corn and Soybean Colonization Ability
Publication date: Available online 20 September 2017 Source:Microbiological Research Author(s): Bruna Durante Batista, Paulo Teixeira Lacava, Andrea Ferrari, Natalia de Sousa Teixeira e Silva, Maria Letícia Bonatelli, Sarina Tsui, Mateus Mondin, Elliot Watanabe Kitajima, José Odair Pereira, João Lúcio Azevedo, Maria Carolina Quecine The present study assessed the plant growth-promoting (PGP) traits and diversity of culturable rhizobacteria associated with guarana (Paullinia cupana), a typical tropical plant. Ninety-six bacteria were isolated, subjected to biochemical tests, and identified by pa...
Source: Microbiological Research - September 20, 2017 Category: Infectious Diseases Source Type: research

Distribution of apple and blackcurrant microbiota in Lithuania and the Czech Republic
In this study, we report Next Generation Sequencing-based metagenomic analysis of microbial communities associated with apple and blackcurrant fruits harvested from Lithuania and the Czech Republic. Differences in the taxonomic composition of eukaryotic and prokaryotic microorganisms were observed between plant types. Our results revealed limited geographic differentiation between the bacterial and fungal communities associated with apples. In contrast, blackcurrant berries harvested from different regions demonstrated high diversity in both bacterial and fungal microbiota structures. Among fungal and bacterial microorgani...
Source: Microbiological Research - September 14, 2017 Category: Infectious Diseases Source Type: research

Combination of amplified rDNA restriction analysis and high-throughput sequencing revealed the negative effect of colistin sulfate on the diversity of soil microorganisms
In this study, we determined the effect of colistin sulfate on the diversity of soil microorganisms by amplified rDNA restriction analysis (ARDRA) and high-throughput sequencing.ARDRAshowed that the diversity of DNA from soil microorganisms was reduced after soil was treated with colistin sulfate, with the most dramatic reductionobserved after 35days of treatment. High-throughput sequencing showed that the Chao1 and abundance-based coverage estimators (ACE) were reduced in the soils treated with colistin sulfate for 35 dayscompared to those treated with colistin sulfate for 7 days. Furthermore, Chao1 and ACE tended to be l...
Source: Microbiological Research - September 14, 2017 Category: Infectious Diseases Source Type: research

Characterization and assessment of two biocontrol bacteria against Pseudomonas syringae wilt in Solanum lycopersicum and its genetic responses
Publication date: Available online 12 September 2017 Source:Microbiological Research Author(s): Kaliannan Durairaj, Palanivel Velmurugan, Jung-Hee Park, Woo-Suk Chang, Yool-Jin Park, Planinaicker Senthilkumar, Kyung-Min Choi, Jeong-Ho Lee, Byung-Taek Oh Pseudomonas and Bacillus species are attractive due to their potential bio-control application against plant bacterial pathogens. Pseudomonas aeruginosa strain D4 and Bacillus stratosphericus strain FW3 were isolated from mine tailings in South Korea. In these potent bacterial strains, we observed improved antagonistic activity against Pseudomonas syringae DC3000. These st...
Source: Microbiological Research - September 13, 2017 Category: Infectious Diseases Source Type: research

Assessment of active bacteria metabolizing phenolic acids in the peanut (Arachis hypogaea L.) rhizosphere
The objectives of this study were to isolate and characterize bacteria capable of growth on benzoic and vanillic acids as the sole carbon source in the peanut rhizosphere. Twenty-four bacteria were isolated, and the identification based on 16S rRNA gene sequencing revealed that pre-exposure to phenolic acids before sowing shifted the dominant culturable bacterial degraders from Arthrobacter to Burkholderia stabilis-like isolates. Both Arthrobacter and B. stabilis-like isolates catalysed the aromatic ring cleavage via the ortho pathway, and Arthrobacter isolates did not exhibit higher C12O enzyme activity than B. stabilis-l...
Source: Microbiological Research - September 10, 2017 Category: Infectious Diseases Source Type: research

Nucleoside diphosphate kinase (Ndk): A pleiotropic effector manipulating bacterial virulence and adaptive responses
Publication date: Available online 8 September 2017 Source:Microbiological Research Author(s): Hua Yu, Xiancai Rao, Kebin Zhang Nucleoside diphosphate kinase (Ndk) is a housekeeping enzyme that balances cellular nucleoside triphosphate (NTP) pools by catalyzing the reversible transfer of γ-phosphate from NTPs to nucleoside diphosphates (NDPs). In addition to its fundamental role in nucleotide metabolism, Ndk has roles in protein histidine phosphorylation, DNA cleavage/repair, and gene regulation. Recent studies have also revealed that Ndk secreted from bacteria is important in modulating virulence-associated phenoty...
Source: Microbiological Research - September 9, 2017 Category: Infectious Diseases Source Type: research

Community composition specificity and potential role of phosphorus solubilizing bacteria attached on the different bloom-forming cyanobacteria
Publication date: Available online 31 August 2017 Source:Microbiological Research Author(s): Liu Yang, Yuqian Liu, Xiuyun Cao, Zijun Zhou, Siyang Wang, Jian Xiao, Chunlei Song, Yiyong Zhou The abundance, phosphorus solubilizing ability and community composition of phosphorus solubilizing bacteria (PSB) attached on two bloom-forming cyanobacteria, Microcystis and Anabeana, were investigated in Guanqiao ponds in 2014 and Lake Chaohu in 2015 and 2016. Thirty organic phosphate-mineralizing bacteria (OPB) and eighteen inorganic phosphate-solubilizing bacteria (IPB) isolated from Guanqiao ponds and Lake Chaohu were identified. ...
Source: Microbiological Research - September 1, 2017 Category: Infectious Diseases Source Type: research

Trichosporon asahii secretes a 30-kDa aspartic peptidase
Publication date: Available online 30 August 2017 Source:Microbiological Research Author(s): Roberta S. Valle, Lívia S. Ramos, Vanessa J. Reis, Mariangela Ziccardi, Marcos Dornelas-Ribeiro, Cátia L. Sodré, Marta H. Branquinha, André L.S. Santos Trichosporon asahii is a fungal opportunistic pathogen that causes superficial and deep-seated infections presenting high mortality. Very little is known about the virulence attributes produced by this fungus. Herein, aspartic peptidase production was identified in Brazilian clinical isolates of T. asahii by different methodologies. Initially, T. asahii ...
Source: Microbiological Research - August 31, 2017 Category: Infectious Diseases Source Type: research

Differential Antagonistic Responses of Bacillus pumilus MSUA3 against Rhizoctonia solani and Fusarium oxysporum Causing Fungal Diseases in Fagopyrum esculentum Moench
Publication date: Available online 30 August 2017 Source:Microbiological Research Author(s): Mohit Agarwal, Shrivardhan Dheeman, Ramesh Chand Dubey, Pradeep Kumar, Dinesh Kumar Maheshwari, Vivek K. Bajpai Chitinase and surfactin-mediated biocontrol of Rhizoctonia solani and Fusarium oxysporum causing wilt and root rot of Fagopyrum esculentum respectively has been studied in this communication. Bacillus pumilus MSUA3 as a potential bacterial strain strongly inhibited the growth of R. solani and F. oxysporum involving the chitinolytic enzymes and an antibiotic surfactin. Plant growth promoting attributes seem to be involved...
Source: Microbiological Research - August 31, 2017 Category: Infectious Diseases Source Type: research

Assessment of two carrier materials for phosphate solubilizing biofertilizers and their effect on growth of wheat (Triticum aestivum L.)
Publication date: Available online 30 August 2017 Source:Microbiological Research Author(s): Salma Mukhtar, Izzah Shahid, Samina Mehnaz, Kauser A Malik Biofertilizers are usually carrier-based inoculants containing beneficial microorganisms. Incorporation of microorganisms in carrier material enables easy-handling, long-term storage and high effectiveness of biofertilizers. Objective of the present study was to assess enriched biogas sludge and soil as biofertilizer carriers on growth and yield of wheat. Six phosphate solubilizing strains were used in this study. Three phosphate solubilizing strains, 77-NS2 (Bacillus endo...
Source: Microbiological Research - August 31, 2017 Category: Infectious Diseases Source Type: research

A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase
We present for the first time the cloning, expression, purification of mycobacterial GFP-tagged BirA and its application for the development of a high-throughput assay building on the principle of differential scanning fluorimetry of GFP-tagged proteins. The data obtained in this study reveal how biotin and ATP significantly increase the thermal stability (ΔT m =+16.5°C) of M. tuberculosis BirA and lead to formation of a high affinity holoenzyme complex (Kobs =7.7nM). The new findings and mycobacterial BirA high-throughput assay presented in this work could provide an efficient platform for future anti-tubercular...
Source: Microbiological Research - August 31, 2017 Category: Infectious Diseases Source Type: research

Analysis of the Pho regulon in Streptomyces tsukubaensis
Publication date: Available online 24 August 2017 Source:Microbiological Research Author(s): María Ordóñez-Robles, Fernando Santos-Beneit, Antonio Rodríguez-García, Juan F. Martín Phosphate regulation of antibiotic biosynthesis in Streptomyces has been studied due to the importance of this genus as a source of secondary metabolites with biological activity. Streptomyces tsukubaensis is the main producer of tacrolimus (or FK506), an immunosuppressant macrolide that generates important benefits for the pharmaceutical market. However, the production of tacrolimus is under a negative ...
Source: Microbiological Research - August 25, 2017 Category: Infectious Diseases Source Type: research

Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in Vibrio anguillarum against cold stress
Publication date: December 2017 Source:Microbiological Research, Volume 205 Author(s): Yue Ma, Qiyao Wang, Wensheng Xu, Xiaohong Liu, Xiating Gao, Yuanxing Zhang The capability of cold-adaptation is a prerequisite of microorganisms that survive in an environment with frequent fluctuations in temperature. As a global causative agent of vibriosis in marine fish farming, Vibrio anguillarum can efficiently grow and proliferate under cold-stress conditions, which is 15°C lower than the optimal growth temperatures (25–30°C). Our data showed that V. anguillarum was able to synthesize ectoine de novo and that ectoin...
Source: Microbiological Research - August 25, 2017 Category: Infectious Diseases Source Type: research

Comparative Genomics of Lactobacillus salivarius Strains Focusing on Their Host Adaptation
In this study, we generated 21 L. salivarius draft genomes, and investigated the pan-genome of L. salivarius strains isolated from humans, pigs and chickens using all available genomes, focusing on host adaptation. Phylogenetic clustering showed a distinct categorization of L. salivarius strains depending on their hosts. In the pan-genome, 15 host-specific genes and 16 dual-host-shared genes that only one host isolate did not possess were identified. Comparison of 56 extracellular protein encoding genes and 124 orthologs related to exopolysaccharide production in the pan-genome revealed that extracellular components of the...
Source: Microbiological Research - August 24, 2017 Category: Infectious Diseases Source Type: research

Exploitation of agro-climatic environment for selection of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase producing salt tolerant indigenous plant growth promoting rhizobacteria
Publication date: Available online 24 August 2017 Source:Microbiological Research Author(s): Sankalp Misra, Vijay Kant Dixit, Mohammad Haneef Khan, Shashank Kumar Mishra, Gyanendra Dviwedi, Sumit Yadav, Alok Lehri, Puneet Singh Chauhan A comprehensive survey for 09 agro-climatic zones of Uttar Pradesh, India was conducted to isolate and characterize salt tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase possessing plant growth promoting (PGP) rhizobacteria for salt stress amelioration in rice. Here, we have isolated 1125 bacteria having the ability to tolerate 1M NaCl and out of those, 560 were screened for u...
Source: Microbiological Research - August 24, 2017 Category: Infectious Diseases Source Type: research

Osmoprotective functions conferred to soybean plants via inoculation with Sphingomonas sp. LK11 and exogenous trehalose
In conclusion, our findings demonstrated that inoculation with this endophyte and trehalose improved the negative effects of drought-induced osmotic stress, and it enhanced soybean plant growth and tolerance. (Source: Microbiological Research)
Source: Microbiological Research - August 24, 2017 Category: Infectious Diseases Source Type: research

A novel soxO gene, encoding a glutathione disulfide reductase, is essential for tetrathionate oxidation in Advenella kashmirensis
Publication date: Available online 12 August 2017 Source:Microbiological Research Author(s): Prosenjit Pyne, Masrure Alam, Wriddhiman Ghosh Molecular mechanisms of chemolithotrophic tetrathionate oxidation are not clearly understood. Here we used transposon(Tn5-mob)-insertion mutagenesis to search for novel tetrathionate oxidation genes in the facultatively chemolithoautotrophic betaproteobacterium Advenella kashmirensis that not only oxidizes tetrathionate, but also produces the same as an intermediate during thiosulfate oxidation. Genome-wide random insertion of Tn5-mob occurred at a frequency of one per 104 donor E. co...
Source: Microbiological Research - August 18, 2017 Category: Infectious Diseases Source Type: research

Characterization and complete genome sequences of L. rhamnosus DSM 14870 and L. gasseri DSM 14869 contained in the EcoVag ® probiotic vaginal capsules
Publication date: Available online 14 August 2017 Source:Microbiological Research Author(s): Harold Marcotte, Kasper Krogh Andersen, Yin Lin, Fanglei Zuo, Zhu Zeng, Per Göran Larsson, Erik Brandsborg, Gunnar Brønstad, Lennart Hammarström Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag® probiotic capsules. EcoVag® was subsequently shown to provide long-term cure and reduce relapse of bacterial vaginosis (BV) as an adjunct to antibiotic therap...
Source: Microbiological Research - August 18, 2017 Category: Infectious Diseases Source Type: research

Apoptosis induced by Staphylococcus aureus toxins
Publication date: Available online 18 August 2017 Source:Microbiological Research Author(s): Xiaopeng Zhang, Xiaomei Hu, Xiancai Rao Apoptosis stimulated by bacterial toxins is common during infection and is now considered important in disease processes. As a major human pathogen, Staphylococcus aureus also causes apoptosis during infection. In some diseases such as atopic dermatitis and sepsis, the apoptosis induced by S. aureus influences the severity and outcome of diseases. S. aureus has various toxins, many of which have reportedly triggered apoptosis. In this review, we focused on the apoptosis-inducing toxins secre...
Source: Microbiological Research - August 18, 2017 Category: Infectious Diseases Source Type: research

Exploring the Interaction Network of the Bacillus subtilis Outer Coat and Crust Proteins
In this study we examined the interactions between the proteins localized to the outer coat and crust using a bacterial two hybrid system. These two layers are composed of at least 25 components. Self-interactions were observed for most proteins and numerous novel interactions were identified. The most interesting contacts are those made with the morphogenetic proteins CotE, CotY and CotZ; these could serve as a basis for understanding the specific roles of particular proteins in spore coat morphogenesis. (Source: Microbiological Research)
Source: Microbiological Research - August 9, 2017 Category: Infectious Diseases Source Type: research

Characterization of multifarious plant growth promoting traits of rhizobacterial strain AR6 under Chromium (VI) stress
Publication date: Available online 29 July 2017 Source:Microbiological Research Author(s): Chinnannan Karthik, Namasivayam Elangovan, Thamilarasan Senthil Kumar, Subramani Govindharaju, Selvaraj Barathi, Mohammad Oves, Padikasan Indra Arulselvi Plant growth promoting rhizobacteria (PGPR) can increase the host plant tolerance to cope up with heavy metal induced stress, which can be improve plant growth. Thus, the present study was designed to isolate Cr(VI) tolerant PGPR strain and evaluate its plant growth promoting (PGP) properties under Cr(VI) stress. Rhizobacterial strain AR6 was isolated from the rhizosphere of Phaseo...
Source: Microbiological Research - July 30, 2017 Category: Infectious Diseases Source Type: research

Genomic characterization of an extensively-drug resistance Salmonella enterica serotype Indiana strain harboring blaNDM-1 gene isolated from a chicken carcass in China
Conclusions This study demonstrated that multi-resistance mechanisms consistent with an XDR-phenotype, along with various virulence encoding genes of a S. Indiana strain in China These findings highlight the importance of cooperation among different sectors in order to monitor the spread of resistant pathogens among food animal, foods of animal origin and human beings that might further take measures to protect consumers’ health. (Source: Microbiological Research)
Source: Microbiological Research - July 28, 2017 Category: Infectious Diseases Source Type: research

Ethanol production from chitosan by the nematophagous fungus Pochonia chlamydosporia and the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
Publication date: Available online 25 July 2017 Source:Microbiological Research Author(s): Almudena Aranda-Martinez, Miguel Ángel Naranjo Ortiz, Isabel Sofía Abihssira García, Ernesto A. Zavala-Gonzalez, Luis Vicente Lopez-Llorca Ethanol production is mainly obtained from plant biomass. Chitin is the second most abundant biopolymer after cellulose and virtually unexplored as raw material for bioethanol production. In this paper, we investigate chitosan, the deacetylated form of chitin which is the main component of shellfish waste, as substrate for bioethanol production by fungi. Fungal parasites of i...
Source: Microbiological Research - July 26, 2017 Category: Infectious Diseases Source Type: research

Functional characterization of the Dsc E3 ligase complex in the citrus postharvest pathogen Penicillium digitatum
In this study, five genes, designated PddscA, PddscB, PddscC, PddscD, and PddscE encoding the Dsc E3 ligase complex were characterized to be required for fungicide resistance, ergosterol biosynthesis and CoCl2 tolerance in P. digitatum. Each of the dsc genes was inactivated by target gene disruption and the resulted phenotypes were analyzed and compared. Genetic analysis reveals that, of five Dsc complex components, PddscB is the core subunit gene in P. digitatum. Although the resultant dsc mutants were able to infect citrus fruit and induce maceration lesions as the wild-type, the mutants rarely produced aerial mycelia on...
Source: Microbiological Research - July 22, 2017 Category: Infectious Diseases Source Type: research

Targets of ubiquitin like system in mycobacteria and related actinobacterial species
Publication date: Available online 18 July 2017 Source:Microbiological Research Author(s): Yusuf Akhter, Shweta Thakur Protein turnover and recycling is a prerequisite in all living organisms to maintain normal cellular physiology. Many bacteria are proteasome deficient but they possess typical protease enzymes for carrying out protein turnover. However, several groups of actinobacteria such as mycobacteria harbor both proteasome and proteases. In these bacteria, for cellular protein, turnover the target proteins undergo post-translational modification referred as pupylation in which a small protein Pup (prokaryotic ubiqu...
Source: Microbiological Research - July 19, 2017 Category: Infectious Diseases Source Type: research

The yeast-like fungus Aureobasidium thailandense LB01 produces a new biosurfactant using olive oil mill wastewater as an inducer
In this study, the biosurfactant production by an Aureobasidium thailandense LB01 was reported for the first time. Different agro-industrial by-products (corn steep liquor, sugarcane molasses, and olive oil mill wastewater) were evaluated as alternative low-cost substrates. The composition of the culture medium was optimized through response surface methodology. The highest biosurfactant production (139±16mg/L) was achieved using a culture medium containing yeast extract (2g/L); olive oil mill wastewater (1.5%, w/w); glucose (6g/L) and KH2PO4 (1g/L) after 48h of fermentation. The partially purified biosurfactant exh...
Source: Microbiological Research - July 18, 2017 Category: Infectious Diseases Source Type: research

Nutrients and host attributes modulate the abundance and functional traits of phyllosphere microbiome in rice
Publication date: Available online 17 July 2017 Source:Microbiological Research Author(s): Shobit Thapa, Radha Prasanna, Kunal Ranjan, Kulandaivelu Velmourougane, Balasubramanian Ramakrishnan The abundance of phyllosphere bacterial communities of seven genotypes of rice −ADT- 38, ADT-43, CR-1009, PB-1, PS-5, P-44, and Pusa Basmati was investigated, in relation to nutrient dynamics of rhizosphere and leaves. P-44 genotype recorded highest pigment accumulation, while genotypes CR-1009 and P-44 exhibited most number of different bacterial morphotypes, Colony forming units in two media (Nutrient agar and R2A) varied sig...
Source: Microbiological Research - July 18, 2017 Category: Infectious Diseases Source Type: research

Identification and preliminary characterization of a putative C-type lectin receptor-like protein in the T. cruzi tomato lectin endocytic-enriched proteome
Publication date: Available online 12 July 2017 Source:Microbiological Research Author(s): Sébastien Brosson, Guy Bottu, Etienne Pays, Sabrina Bousbata, Didier Salmon Trypanosoma cruzi, the etiological agent of the Chagas’ disease in Latin America undergoes a complex life cycle involving two hosts, a mammalian host and a reduviid insect vector (triatomine). In the insect midgut the parasite multiplies as epimastigote forms, which rely on endocytosis for their energy requirement. We recently showed that posttranslational modification of endocytic N-glycoproteins by tomato lectin (TL) binding-N-glycans is cruci...
Source: Microbiological Research - July 13, 2017 Category: Infectious Diseases Source Type: research

Volatiles emitted by Bacillus sp. BCT9 act as growth modulating agents on Lactuca sativa seedlings
In this study, VOCs released by Bacillus sp. BCT9 cultures grown in different media (Methyl Red & Voges Proskauer, Murashige & Skoog and nutrient media) at concentrations of 0.1, 0.2, 0.5 and 0.7 (measured as the absorbance, λ=600nm) were tested to evaluate their activity as growth inducers of L. sativa after 10days of exposure. Lower concentrations of BCT9 increased root length, and higher concentrations induced shoot length and lateral root length. The dry weight and number of lateral roots increased similarly, independent of concentration, for VOCs produced in all culture media. BCT9 cultures grow...
Source: Microbiological Research - July 12, 2017 Category: Infectious Diseases Source Type: research

Involvement of Penicillium digitatum PdSUT1 in fungicide sensitivity and virulence during citrus fruit infection.
Publication date: Available online 10 July 2017 Source:Microbiological Research Author(s): Marta de Ramón-Carbonell, Paloma Sánchez-Torres A putative sucrose transporter PdSUT1 included in the same clade that Sut1p from Schizosaccharomyces pombe was identified in Penicillium digitatum, the major citrus postharvest pathogen. PdSUT1 gene was characterized using target gene disruption and gene overexpression. The ΔPdSUT1 mutants generated by gene elimination showed reduction in fungal virulence during citrus fruit infection assayed in mature fruit at 20°C. However, the overexpression mutants did not i...
Source: Microbiological Research - July 11, 2017 Category: Infectious Diseases Source Type: research

Influence of (p)ppGpp on biofilm regulation in Pseudomonas putida KT2440
Publication date: Available online 8 July 2017 Source:Microbiological Research Author(s): Huizhong Liu, Yujie Xiao, Hailing Nie, Qiaoyun Huang, Wenli Chen The global regulatory molecule (p)ppGpp is synthesized under limited nutrition conditions and involves in many cellular processes in bacteria. (p)ppGpp has been reported to affect biofilm formation in several bacterial species. Here, we found that deletion of (p)ppGpp synthase genes of Pseudomonas putida KT2440 led to enhanced biofilm formation in polystyrene microtitre plates. Besides, the pellicle of this mutant formed at the air-liquid interface lost the robust struc...
Source: Microbiological Research - July 9, 2017 Category: Infectious Diseases Source Type: research

Improving gentamicin B and gentamicin C1a production by engineering the glycosyltransferases that transfer primary metabolites into secondary metabolites biosynthesis
Publication date: Available online 1 July 2017 Source:Microbiological Research Author(s): Zheng Wu, Wenli Gao, Shaotong Zhou, Zhaolin Wen, Xianpu Ni, Huanzhang Xia Gentamicin B and gentamicin C1a are the direct precursor for Isepamicin and Etimicin synthesis, respectively. Although producing strains have been improved for many years, both gentamicin B titer and gentamicin C1a titer in the fermentation are still low. Because all gentamicin components are biosynthesized using UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and UDP-xylose as precursors, we tried to explore strategies for development of strains capable of directing g...
Source: Microbiological Research - July 1, 2017 Category: Infectious Diseases Source Type: research

Bacterial magnetosome and its potential application
This article also highlights recent advances in the understanding of the biochemical and magnetic characteristics of bacterial magnetosome, as well as the magnetosome formation mechanism including iron ions uptake, magnetosome membrane formation, biomineralization and magnetosome chain assembly. Finally, this review presents the potential applications of bacterial magnetosome in biomedicine, wastewater treatment, and the significance of mineralization of magnetosome in biology and geology. (Source: Microbiological Research)
Source: Microbiological Research - June 21, 2017 Category: Infectious Diseases Source Type: research

Arsenic inorganic compounds cause oxidative stress mediated by the transcription factor PHO4 in Candida albicans
Publication date: Available online 17 June 2017 Source:Microbiological Research Author(s): Verónica Urrialde, Begoña Alburquerque, José Pedro Guirao-Abad, Jesús Pla, Juan Carlos Argüelles, Rebeca Alonso-Monge Arsenic is a toxic metalloid widespread in nature. Recently, it has been demonstrated a main role of the transcription factor Pho4 in the acquisition of tolerance to arsenic-derived compounds, arsenite and arsenate in Candida albicans. Here, the effect of these compounds on this pathogenic yeast has been analyzed. In wild type cells, both arsenite and arsenate induced a marked increas...
Source: Microbiological Research - June 20, 2017 Category: Infectious Diseases Source Type: research

The G α1-cAMP Signaling Pathway Controls Conidiation, Development and Secondary Metabolism in the taxol-producing fungus Pestalotiopsis microspora
In this study, we report a gene, pgα1, that putatively encodes the α-subunit of a group I G protein in Pestalotiopsis microspora NK17, which is known to produce various secondary metabolites, including the antitumor drug taxol and pestalotiollide B (PB). Mutants of pgα1 showed retarded vegetative growth, aging of the mycelium, premature conidiation, deformed conidia, significantly increased melanin production, and a sharp decrease in PB production. The introduction of extra copies of pgα1 led to a different phenotype that was characterized by enhanced production of PB. qRT-PCR revealed that the expr...
Source: Microbiological Research - June 16, 2017 Category: Infectious Diseases Source Type: research

Isogenic mutations in the Moraxella catarrhalis CydDC system display pleiotropic phenotypes and reveal the role of a palindrome sequence in its transcriptional regulation
Publication date: Available online 9 June 2017 Source:Microbiological Research Author(s): Yosra I. Nagy, Manal M.M. Hussein, Yasser M. Ragab, Ahmed S. Attia Moraxella catarrhalis is becoming an important human respiratory tract pathogen affecting significant proportions from the population. However, still little is known about its physiology and molecular regulation. To this end, the CydDC, which is a heterodimeric ATP binding cassette transporter that has been shown to contribute to the maintenance of the redox homeostasis across the periplasm in other Gram-negative bacteria, is studied here. Amino acids multiple sequenc...
Source: Microbiological Research - June 10, 2017 Category: Infectious Diseases Source Type: research

Zinc solubilizing Bacillus spp. potential candidates for biofortification in maize
This study indicated that inoculation of Zn solubilizing strains have potential to promote growth and can be the potential bio-inoculants for biofortification of maize to overcome the problems of malnutrition. (Source: Microbiological Research)
Source: Microbiological Research - June 9, 2017 Category: Infectious Diseases Source Type: research

Effect of succinate on phosphate solubilization in nitrogen fixing bacteria harbouring chick pea and their effect on plant growth
Publication date: Available online 30 May 2017 Source:Microbiological Research Author(s): Bhagya Iyer, Mahendrapal Singh Rajput, Shalini Rajkumar Diverse nitrogen fixing bacteria harbouring chick pea rhizosphere and root nodules were tested for multiple plant growth promoting traits like tricalcium phosphate (TCP) and rock phosphate (RP) solubilization, production of ammonia, indole 3-acetic acid, chitinase, phytase and alkaline phosphatase. Isolates belonged to diverse genus like Enterobacter, Acinetobacter, Erwinia, Pseudomonas, Rhizobium, Sinorhizobium, Ensifer, Klebsiella, etc. Most isolates solubilized TCP and RP alo...
Source: Microbiological Research - May 31, 2017 Category: Infectious Diseases Source Type: research

An improved strategy for stable biocontrol agents selecting to control rice sheath blight caused by Rhizoctonia solani
Publication date: Available online 30 May 2017 Source:Microbiological Research Author(s): Yi-Yang Yu, Chun-Hao Jiang, Chao Wang, Liu-Jun Chen, Hong-Yang Li, Quan Xu, Jian-Hua Guo Rice sheath blight caused by Rhizoctonia solani Kühnis increasingly threatening rice production in China. DNA fingerprints of 220 R. solani strains isolated in 11 provinces of China were established by random amplified polymorphic DNA (RAPD)-PCR. Cluster analysis of strains isolated from the same region showed high similarity, indicating that the genetic diversity of R. solani strains is significantly related to geographical origin. We asses...
Source: Microbiological Research - May 30, 2017 Category: Infectious Diseases Source Type: research

DNA-based stable isotope probing identifies formate-metabolizing methanogenic archaea in paddy soil
Publication date: Available online 26 May 2017 Source:Microbiological Research Author(s): Jiangbing Xu, Zhongjun Jia, Xiangui Lin, Youzhi Feng Paddy methane (CH4) production is biologically dominated by methanogenic archaea that metabolize a variety of organic and/or inorganic carbon sources. Though formate is easily dissimilated into H2/CO2, formate-metabolizing methanogenic archaea are distinct from CO2-utilizing methanogen taxa. The identity of formate-metabolizing methanogenic archaea in paddy soil remains elusive. In this investigation, molecular approaches based on stable isotope probing (SIP) technique were conduct...
Source: Microbiological Research - May 27, 2017 Category: Infectious Diseases Source Type: research

Putative Protein VC0395_0300 from Vibrio cholerae is a Diguanylate Cyclase with a Role in Biofilm Formation
Publication date: Available online 23 May 2017 Source:Microbiological Research Author(s): Divya Bandekar, Om Prakash Chouhan, Swati Mohapatra, Mousumi Hazra, Saugata Hazra, Sumit Biswas The hallmark of the lifecycle of Vibrio cholerae is its ability to switch between two lifestyles − the sessile, non-pathogenic form and the motile, infectious form in human hosts. One of these changes is in the formation of surface biofilms, when in sessile aquatic habitats. The cell-cell interactions within a V. cholerae biofilm are stabilized by the production of an exopolysachharide (EPS) matrix, which in turn is regulated by the ...
Source: Microbiological Research - May 24, 2017 Category: Infectious Diseases Source Type: research