Isolation of Trichoderma from forestry model base and the antifungal properties of isolate TpsT17 toward Fusarium oxysporum
Publication date: Available online 5 November 2019Source: Microbiological ResearchAuthor(s): Chang Zhou, Ruiting Guo, Shida Ji, Haijuan Fan, Jinjie Wang, Yucheng Wang, Zhihua LiuAbstractEleven soil samples were collected from different plantations at the Forestry Model Base, Northeast Forestry University, China (45°43ʹ10″ N, 126°37ʹ15″ E), and 122 Trichoderma strains (T1–T122) were isolated. Nine Trichoderma species were identified based on morphological and molecular classification methods. The diversity of woody fungi was analyzed based on the type and quantity of Trichoderma spp. in the soil ...
Source: Microbiological Research - November 7, 2019 Category: Infectious Diseases Source Type: research

The potential of endophytic fungi isolated from cucurbit plants for biocontrol of soilborne fungal diseases of cucumber
In this study, 82 endophytic fungal strains had good disease suppressive effects and no obvious adverse effects on cucumber growth, and 35 of them showed obvious growth-promoting effects, which suggested that endophytic fungi from cucurbit plants have excellent potential for plant disease control. (Source: Microbiological Research)
Source: Microbiological Research - November 5, 2019 Category: Infectious Diseases Source Type: research

Illumina sequencing of 16S rRNA tag shows disparity in rhizobial and non-rhizobial diversity associated with root nodules of mung bean (Vigna radiata L.) growing in different habitats in Pakistan
Publication date: Available online 23 October 2019Source: Microbiological ResearchAuthor(s): Sughra Hakim, Babur S. Mirza, Asma Imran, Ahmad Zaheer, Sumera Yasmin, Fathia Mubeen, Joan E. Mclean, M. Sajjad MirzaAbstractIn Rhizobium-legume symbiosis, the nodule is the most frequently studied compartment, where the endophytic/symbiotic microbiota demands critical investigation for development of specific inocula. We identified the bacterial diversity within root nodules of mung bean from different growing areas of Pakistan using Illumina sequencing of 16S rRNA gene. We observed specific OTUs related to specific site where Bra...
Source: Microbiological Research - October 24, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: December 2019Source: Microbiological Research, Volume 229Author(s): (Source: Microbiological Research)
Source: Microbiological Research - October 23, 2019 Category: Infectious Diseases Source Type: research

CpALS4770 and CpALS4780 contribution to the virulence of Candida parapsilosis
Publication date: Available online 18 October 2019Source: Microbiological ResearchAuthor(s): Marina Zoppo, Mariagrazia Di Luca, Mauro Franco, Cosmeri Rizzato, Antonella Lupetti, Annarita Stringaro, Flavia De Bernardis, Christoph Schaudinn, M. Inmaculada Barrasa, Daria Bottai, Valmik K. Vyas, Arianna TavantiAbstractThe ability of yeast to adhere to biotic and abiotic surfaces represents an essential trait during the early stages of infection. Agglutinin-like sequence (Als) cell-wall proteins play a key role in adhesion of Candida species. Candida parapsilosis genome encompasses 5 ALS members, of which only the role of CPAR2...
Source: Microbiological Research - October 19, 2019 Category: Infectious Diseases Source Type: research

Arbuscular mycorrhizal fungi (Glomus intraradices) and diazotrophic bacterium (Rhizobium BMBS) primed defense in blackgram against herbivorous insect (Spodoptera litura) infestation
Publication date: Available online 17 October 2019Source: Microbiological ResearchAuthor(s): Selvaraj Anandakumar, Thangavel Kalaiselvi, Uthandi SivakumarAbstractIn the changing scenario of agriculture, plants are exposed to various biotic and abiotic stresses. Induction of both constitutive and inducible defense systems was noticed in plants exposed to stress. As a major defense response, production of phenolics and superoxide radicals quenching enzymes is accelerated in plants under stress. These metabolites production intensified further when arbuscular mycorrhizal fungi (AMF) infected plants are subjected to stress. Wi...
Source: Microbiological Research - October 18, 2019 Category: Infectious Diseases Source Type: research

Molecular and morphological characterization of rice phylloplane fungi and determination of the antagonistic activity against rice pathogens
Publication date: Available online 15 October 2019Source: Microbiological ResearchAuthor(s): Amanda A. Chaibub, Thatyane P. de Sousa, Leila G. de Araújo, Marta Cristina C. de FilippiAbstractCladosporium spp. is a cosmopolitan fungal genus. In the literature, it has been reported as a biological agent for controlling several plant diseases, but its mechanism of action has never been clarified. The present study aims to identify Cladosporium spp. based on the DNA phylogeny of nine isolates obtained from the phylloplane of rice and their potential antagonistic activity against the main fungal pathogens that affect rice...
Source: Microbiological Research - October 16, 2019 Category: Infectious Diseases Source Type: research

Elucidation of quorum sensing components and their role in regulation of symbiotically important traits in Ensifer nodulating pigeon pea
This study highlights that Ensifer nodulating pigeon pea, although with significant variance in the anatomy of their QS components, regulate symbiotically crucial cell-processes via QS in a scheme that is conserved in multiple genera. (Source: Microbiological Research)
Source: Microbiological Research - October 15, 2019 Category: Infectious Diseases Source Type: research

Deactivation and mislocalization of Toxoplasma gondii rhoptry protein 18 induced by a single amino acid mutation on the proton transport catalytic aspartic acid
Publication date: Available online 13 October 2019Source: Microbiological ResearchAuthor(s): Yang Zhang, Bo Shiun Lai, Mario JuhasAbstractRhoptry protein 18 (ROP18) is a major determinant of strain-specific virulence in Toxoplasma gondii. The kinase activity of ROP18 is required for acute virulence, while the aspartate in the catalytic loop of ROP18 is considered essential for phosphoryl transfer. We showed that a single amino acid mutation at the catalytic aspartate residue (D409A mutation) significantly altered ROP18 kinase activity in vitro, and abolished ROP18-mediated ATF6β degradation. Furthermore, the investiga...
Source: Microbiological Research - October 14, 2019 Category: Infectious Diseases Source Type: research

Outer membrane protein OmpU is related to iron balance in Vibrio alginolyticus
Publication date: Available online 11 October 2019Source: Microbiological ResearchAuthor(s): Tengteng Lv, Fa Dai, Qiuting Zhuang, Xuelin Zhao, Yina Shao, Ming Guo, Zhimeng Lv, Chenghua Li, Weiwei ZhangAbstractOuter membrane protein U (OmpU) is a major porin from Vibrio alginolyticus and has been considered a vaccine candidate against infection by V. alginolyticus. After pre-incubated with polyclonal antibody against rOmpU, V. alginolyticus showed a 78% decrease in extracellular iron level, suggesting that interruption of OmpU could increase intracellular iron level. The mRNA expression of ompU under iron-limited conditions...
Source: Microbiological Research - October 12, 2019 Category: Infectious Diseases Source Type: research

PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida
In this study, a pull-down assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida. (Source: ...
Source: Microbiological Research - October 10, 2019 Category: Infectious Diseases Source Type: research

Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum
In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains. In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25–30%) than those of the WT and CK strains. Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only ...
Source: Microbiological Research - October 3, 2019 Category: Infectious Diseases Source Type: research

Inhibition of Rhizoctonia solani RhCh-14 and Pythium ultimum PyFr-14 by Paenibacillus polymyxa NMA1017 and Burkholderia cenocepacia CACua-24: A proposal for biocontrol of phytopathogenic fungi
Publication date: Available online 26 September 2019Source: Microbiological ResearchAuthor(s): Belén Chávez-Ramírez, Jeniffer Chirs Kerber-Díaz, Marí Carmen Acoltzi-Conde, J. Antonio Ibarra, María-Soledad Vásquez-Murrieta, Paulina Estrada-de los SantosAbstractBiocontrol has emerged in recent years as an alternative to pesticides. Given the importance of environmental preservation using biocontrol, in this study two antagonistic bacteria against phytopathogenic fungi were isolated and evaluated. These bacterial strains, identified as Paenibacillus polymyxa NMA1017 and Burkhol...
Source: Microbiological Research - September 28, 2019 Category: Infectious Diseases Source Type: research

Two pathways for thiosulfate oxidation in the alphaproteobacterial chemolithotroph Paracoccus thiocyanatus SST
Publication date: Available online 23 September 2019Source: Microbiological ResearchAuthor(s): Moidu Jameela Rameez, Prosenjit Pyne, Subhrangshu Mandal, Sumit Chatterjee, Masrure Alam, Sabyasachi Bhattacharya, Nibendu Mondal, Jagannath Sarkar, Wriddhiman GhoshAbstractChemolithotrophic bacteria oxidize various sulfur species for energy and electrons, thereby operationalizing biogeochemical sulfur cycles in nature. The best-studied pathway of bacterial sulfur-chemolithotrophy involves direct oxidation of thiosulfate (S2O32-) to sulfate (SO42-) without any free intermediate. This pathway mediated by SoxXAYZBCD is apparently t...
Source: Microbiological Research - September 25, 2019 Category: Infectious Diseases Source Type: research

Altered Metabolomic Profile of Dual-Species Biofilm: Interactions Between Proteus mirabilis and Candida albicans
In this study, we aimed to determine the interspecies interactions between Proteus mirabilis and Candida albicans. Mono and dual-species biofilms were grown in a microtiter plate and metabolomic analysis of the biofilms was performed. The effects of togetherness of two species on the expression levels of candidal virulence genes and urease and swarming activities of P.mirabilis were investigated. The growth of C.albicans was inhibited by P.mirabilis whereas the growth and swarming activity of P.mirabilis were increased by C.albicans. The inhibition of Candida cell growth was found to be biofilm specific. The alteration was...
Source: Microbiological Research - September 25, 2019 Category: Infectious Diseases Source Type: research

The "fighting Wisdom and bravery" of tailed phage and host in the process of adsorption
Publication date: Available online 19 September 2019Source: Microbiological ResearchAuthor(s): Haojie Ge, Maozhi Hu, Ge Zhao, Yi Du, Nannan Xu, Xiang Chen, Xin’an JiaoAbstractIn the process of bacteriophage and bacteria struggle, adsorption is the key factor to determine who is the winner. In this paper, the molecular mechanism of tailed bacteriophage recognition and adsorption to host and the strategy of “fighting wisdom and courage” between them are reviewed. (Source: Microbiological Research)
Source: Microbiological Research - September 20, 2019 Category: Infectious Diseases Source Type: research

Identification of genes regulated by the two-component system response regulator NarP of Actinobacillus pleuropneumoniae via DNA-affinity-purified sequencing
Publication date: Available online 10 September 2019Source: Microbiological ResearchAuthor(s): Qiuhong Zhang, Qi Huang, Qiong Fang, Haotian Li, Hao Tang, Geng Zou, Dong Wang, Siqi Li, Weicheng Bei, Huanchun Chen, Lu Li, Rui ZhouAbstractIdentifying the direct target genes of response regulators (RRs) of a bacterial two-component system (TCS) is critical to understand the roles of TCS in bacterial environmental adaption and pathogenesis. Actinobacillus pleuropneumoniae is an important respiratory bacterial pathogen that causes considerable economic losses to swine industry worldwide. The targets of A. pleuropneumoniae NarP (...
Source: Microbiological Research - September 11, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: November 2019Source: Microbiological Research, Volume 228Author(s): (Source: Microbiological Research)
Source: Microbiological Research - September 11, 2019 Category: Infectious Diseases Source Type: research

A script for initiating molecular biology studies with non-conventional yeasts based on Saccharomycopsis schoenii
Publication date: Available online 9 September 2019Source: Microbiological ResearchAuthor(s): Yeseren Kayacan, Adam Griffiths, Jürgen WendlandAbstractNon-conventional yeasts (NCYs), i.e. all yeasts other than Saccharomyces cerevisiae, are emerging as novel production strains and gain more and more attention to exploit their unique properties. Yet, these yeasts can hardly compete against the advanced methodology and genetic tool kit available for exploiting and engineering S. cerevisiae. Currently, for many NCYs one has to start from scratch to initiate molecular genetic manipulations, which is often time consuming and...
Source: Microbiological Research - September 11, 2019 Category: Infectious Diseases Source Type: research

Abiotic stress resistance, plant growth promotion and antifungal potential of halotolerant bacteria from a Tunisian solar saltern
In this study, 15 halotolerant bacterial strains endowed with important antimicrobial activities were isolated from Sfax solar saltern (Tunisia). All of these strains were characterized by biochemical and molecular tools aiming to investigate their in-vitro and in-vivo antifungal potentialities, plant growth promotion capabilities and metal tolerance abilities under saline stress condition. The 16S rRNA gene sequencing showed that the isolated strains were affiliated to different phylum and three species were described for the first time as plant growth promoting strains (Idiomarina zobelli FMH6v, Nesterenkonia halotoleran...
Source: Microbiological Research - September 9, 2019 Category: Infectious Diseases Source Type: research

Evaluating Glucose and Mannose Profiles in Candida Species Using Quantum Dots Conjugated with Cramoll Lectin as Fluorescent Nanoprobes
Publication date: Available online 8 September 2019Source: Microbiological ResearchAuthor(s): Weslley F. Oliveira, Mariana P. Cabrera, Natália R.M. Santos, Thiago H. Napoleão, Patrícia M.G. Paiva, Rejane P. Neves, Márcia V. Silva, Beate S. Santos, Luana C.B.B. Coelho, Paulo E. Cabral Filho, Adriana Fontes, Maria T.S. CorreiaAbstractGlycoconjugates found on cell walls of Candida species are fundamental for their pathogenicity. Laborious techniques have been employed to investigate the sugar composition of these microorganisms. Herein, we prepared a nanotool, based on the fluorescence of quantum d...
Source: Microbiological Research - September 9, 2019 Category: Infectious Diseases Source Type: research

Broad-spectrum antimicrobial activity of secondary metabolites produced by Serratia marcescens strains
Publication date: Available online 4 September 2019Source: Microbiological ResearchAuthor(s): Tanya Clements, Thando Ndlovu, Wesaal KhanAbstractThe genus Serratia is a predominantly unexplored source of antimicrobial secondary metabolites. The aim of the current study was thus to isolate and evaluate the antimicrobial properties of biosurfactants produced by Serratia species. Forty-nine (n = 34 pigmented; n = 15 non-pigmented) biosurfactant producing Serratia strains were isolated from environmental sources and selected isolates (n = 11 pigmented; n = 11 non-pigmented...
Source: Microbiological Research - September 5, 2019 Category: Infectious Diseases Source Type: research

EPS bound flavins driven mediated electron transfer in thermophilic Geobacillus sp.
Publication date: December 2019Source: Microbiological Research, Volume 229Author(s): Dummi Mahadevan Gurumurthy, Ram Naresh Bharagava, Ashok Kumar, Bhaskar Singh, Muhammad Ashfaq, Ganesh Dattatraya Saratale, Sikandar I. MullaAbstractThrough extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in-situ change in electrochemistry and ...
Source: Microbiological Research - September 3, 2019 Category: Infectious Diseases Source Type: research

Estimating CO2 and VOCs production of Colletotrichum fragariae and Rhizopus stolonifer grown in cold stored strawberry fruit
In conclusion, CO2 and VOCs released by C. fragariae and R. stolonifer on strawberries could infer the presence of anthracnose and soft rot during storage of the fruit at low temperature. (Source: Microbiological Research)
Source: Microbiological Research - September 1, 2019 Category: Infectious Diseases Source Type: research

Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities
Publication date: Available online 31 August 2019Source: Microbiological ResearchAuthor(s): Weifang Xu, Fei Wang, Meng Zhang, Ting Ou, Ruolin Wang, Gary Strobel, Zhonghuai Xiang, Zeyang Zhou, Jie XieAbstractEndophytic bacteria-based biocontrol is regarded as a potential plant disease management strategy. Present study analyzed the diversity of mulberry endophytic bacteria basing on a culture-dependent approach and further evaluated their antimicrobial and plant growth-promoting (PGP) activities. A total of 608 cultivable endophytic bacteria, belonging to 4 phyla and 36 genera, were isolated from four mulberry cultivars hav...
Source: Microbiological Research - September 1, 2019 Category: Infectious Diseases Source Type: research

Edwardsiella induces microtubule-severing in host epithelial cells
Publication date: Available online 26 August 2019Source: Microbiological ResearchAuthor(s): Priyanka Aggarwal, Lifan Wei, Yuping Cao, Qin Liu, Julian A. Guttman, Qiyao Wang, Ka Yin LeungAbstractEdwardsiella bacteria cause economic losses to a variety of commercially important fish globally. Human infections are rare and result in a gastroenteritis-like illness. Because these bacteria are evolutionarily related to other Enterobacteriaceae and the host cytoskeleton is a common target of enterics, we hypothesized that Edwardsiella may cause similar phenotypes. Here we use HeLa and Caco-2 infection models to show that microtub...
Source: Microbiological Research - August 27, 2019 Category: Infectious Diseases Source Type: research

Morphological and molecular aspects of sclerotial development in the phytopathogenic fungus Sclerotinia sclerotiorum
Publication date: Available online 26 August 2019Source: Microbiological ResearchAuthor(s): Bruna Sousa Melo, Aline Raquel Voltan, Walquiria Arruda, Fabyano Alvares Cardoso Lopes, Raphaela Georg, Cirano José UlhoaABSTRACTSclerotinia sclerotiorum (Lib.) de Bary produces a resistance structure called sclerotium, which guarantees its survival in soil for long periods. Morphological and melanization aspects during sclerotial development were evaluated by microscopy and qRT-PCR techniques. S. sclerotiorum produces sclerotia with different phases of maturation and melanization during growth in PDA medium. Using scanning e...
Source: Microbiological Research - August 27, 2019 Category: Infectious Diseases Source Type: research

Regulation of metE+ mRNA Expression by FnrS small RNA in Salmonella enterica serovar Typhimurium
Publication date: Available online 24 August 2019Source: Microbiological ResearchAuthor(s): Chia-Wei Chien, Yu-Feng Chan, Po-Shu Shih, Jung-En Kuan, Ke-Feng Wu, Cindy Wu, Whei-Fen WuAbstractMethionine is critical for variety of metabolic processes in biological organisms, acting as a precursor or intermediate for many final products. The last step for the synthesis of methionine is the methylation of homocysteine, which is catalyzed by MetE. Here, we use Salmonella enterica serovar Typhimurium LT2 to study the regulation of the metE+ gene by an anaerobically induced small non-coding RNA-FnrS, the expression of which is str...
Source: Microbiological Research - August 25, 2019 Category: Infectious Diseases Source Type: research

EPS bound flavins driven mediated electron transfer in thermophilic Geobacillus sp
Publication date: Available online 24 August 2019Source: Microbiological ResearchAuthor(s): Dummi Mahadevan Gurumurthy, Ram Naresh Bharagava, Ashok Kumar, Bhaskar Singh, Muhammad Ashfaq, Ganesh Dattatraya Saratale, Sikandar I. MullaAbstractThrough extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in situ change in electrochemistr...
Source: Microbiological Research - August 25, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: October 2019Source: Microbiological Research, Volume 227Author(s): (Source: Microbiological Research)
Source: Microbiological Research - August 15, 2019 Category: Infectious Diseases Source Type: research

Exploitation of potential bioactive compounds from two soil derived actinomycetes, Streptomyces sp. strain 196 and RI.24
Publication date: Available online 12 August 2019Source: Microbiological ResearchAuthor(s): Prateek Kumar, Aditi Kundu, Munendra Kumar, Renu Solanki, Monisha Khanna KapurAbstractDue to emergence of drug resistant pathogens, nearly all available medicines are becoming ineffective against these life threatening pathogens so there is dire need for the discovery of compounds having unique modes of action. During our previous studies, actinomycetes designated as 196 and RI.24 were isolated, screened for bioactive compounds production and characterized using 16S rRNA gene sequencing. Colony 196 was identified as strain of Strept...
Source: Microbiological Research - August 13, 2019 Category: Infectious Diseases Source Type: research

Phytoremediation of mine tailings by Brassica juncea inoculated with plant growth-promoting bacteria
Publication date: Available online 11 August 2019Source: Microbiological ResearchAuthor(s): José Carlos Mendoza-Hernández, Osmar Rodrigo Vázquez-Delgado, Maribel Castillo-Morales, Jenaro Leocadio Varela-Caselis, Juana Deisy Santamaría-Juárez, Octavio Olivares-Xometl, Janette Arriola Morales, Gabriela Pérez-OsorioAbstractMine tailings represent a serious environmental pollution problem and techniques such as phytoremediation using plant growth-promoting bacteria become an important solution due to their environmentally friendly nature. The study performed using Brassica juncea L. (I...
Source: Microbiological Research - August 12, 2019 Category: Infectious Diseases Source Type: research

Stress-induced adaptations in Salmonella: a ground for shaping its Pathogenesis
Publication date: Available online 11 August 2019Source: Microbiological ResearchAuthor(s): Diana Pradhan, Vidya Devi NegiAbstractMicroorganisms are able to adapt to multiple adverse environmental conditions that facilitate their survival. These microorganisms including bacteria, viruses, algae, fungi, and protozoans are exposed to different abiotic and biotic challenges throughout their life. Adaptations help these organisms to overcome the challenges and evolve as successful pathogens which at the same time might lead to severe disease outcome. The intracellular gram-negative pathogen Salmonella, the causative agent of t...
Source: Microbiological Research - August 12, 2019 Category: Infectious Diseases Source Type: research

MarTrack: A versatile toolbox of mariner transposon derivatives used for functional genetic analysis of bacterial genomes
In this study, we combined the merits of the random mutagenesis of mariner transposon and common efficient reporter marker genes or regulatory elements, mCherry, gfp, luxAB, lacZ, sacBR, and PBAD and antibiotic resistance cassettes to construct a series of derivative transposon vectors, pMmch, pMKGR, pMCGR, pMXKGR, pMLKGR, pMSGR, and pMPR, based on the initial transposon pMar2xT7. The function and effectiveness of the modified transposons were verified by introducing them into E. piscicida EIB202. Based on the toolbox, a transposon insertion mutant library containing approximately 3.0 × 105 distinct mut...
Source: Microbiological Research - August 9, 2019 Category: Infectious Diseases Source Type: research

Identification of plasmid encoded osmoregulatory genes from halophilic bacteria isolated from the rhizosphere of halophytes
Publication date: Available online 8 August 2019Source: Microbiological ResearchAuthor(s): Salma Mukhtar, Samia Ahmad, Aftab Bashir, Samina Mehnaz, Muhammad Sajjad Mirza, Kauser Abdulla MalikAbstractBacterial plasmids carry genes that code for additional traits such as osmoregulation, CO2 fixation, antibiotic and heavy metal resistance, root nodulation and nitrogen fixation. The main objective of the current study was to identify plasmid-conferring osmoregulatory genes in bacteria isolated from rhizospheric and non-rhizospheric soils of halophytes (Salsola stocksii and Atriplex amnicola). More than 55% of halophilic bacter...
Source: Microbiological Research - August 9, 2019 Category: Infectious Diseases Source Type: research

Regulatory rewiring through global gene regulations by PhoB and alarmone (p)ppGpp under various stress conditions
Publication date: Available online 5 August 2019Source: Microbiological ResearchAuthor(s): Varsha Jha, Nishant A. Dafale, Hemant J. PurohitAbstractThe phosphorus availability in soil ranged from
Source: Microbiological Research - August 6, 2019 Category: Infectious Diseases Source Type: research

Development of low-cost plant probiotic formulations of functional endophytes for sustainable cultivation of Coleus forskohlii
Publication date: Available online 5 August 2019Source: Microbiological ResearchAuthor(s): Anthati Mastan, Digeshwar Rane, Syed G. Dastager, C.S. Vivek BabuAbstractDeployment of plant endophytes at field level is reported to make an impact on agricultural crop productivity; development and deployment of suitable crop specific plant probiotics in a suitable delivery matrix is a value-added task. In our study, we attempted to develop bioformulations of native, fungal endophytes of Coleus forskohlii to improve plant yield using two different carrier-based materials (talc and wheat bran). Initially, fungal endophytes (RF1, SF1...
Source: Microbiological Research - August 6, 2019 Category: Infectious Diseases Source Type: research

pdh is involved in the Cell Division and Normal Septation of Streptococcus suis
In this study, we found that deletion of the pdh gene in S. suis resulted in abnormal cell chains, plump morphology and abnormal localization of the Z rings, indicating that the knockout mutant is impaired in its ability to divide. In addition, the interaction between FtsZ and PDH in vitro was confirmed by ELISA, and qRT-PCR analysis revealed that the deletion of the pdh gene results in differential expression of the division-related genes ftsZ, ftsK, ftsl, zapA, divIC, pbp1a, rodA, mreD, and sepF. These results indicate that pdh is involved in the normal formation of Z rings and cell morphology during S. suis cell divisio...
Source: Microbiological Research - August 3, 2019 Category: Infectious Diseases Source Type: research

Neopestalotiopsis species presenting wide dye destaining activity: report of a mycelium-associated laccase
In this study, six endophytic strains isolated from Maytenus ilicifolia were studied for their destaining activity. The phylogenetic and morphological analysis allowed the identification of strain LGMF1504 as Neopestalotiopsis sp. LGMF1504 that decolorized several commercial dyes as the result of a mycelium-associated laccase. The enzyme expression was modulated by carbon and nitrogen content in the culture medium, it was weakly affected by the presence of aromatic compounds and metal ions while some common laccase mediators improved the destaining activity onto dye substrates. The best culture condition observed for lacca...
Source: Microbiological Research - July 26, 2019 Category: Infectious Diseases Source Type: research

Assessment of Predatory Bacteria and Prey Interactions Using Culture-Based Methods and EMA-qPCR
In conclusion, the cell counts and/or EMA-qPCR analysis for the HEPES buffer and DNB assays were successfully employed to monitor the predation of P. fluorescens and K. pneumoniae by B. bacteriovorus, while E. faecium was sensitive to predation in DNB and S. aureus was sensitive to predation in HEPES buffer.Graphical abstract (Source: Microbiological Research)
Source: Microbiological Research - July 26, 2019 Category: Infectious Diseases Source Type: research

Chumacin-1 and Chumacin-2 from Pseudomonas aeruginosa strain CGK-KS-1 as novel quorum sensing signaling inhibitors for biocontrol of bacterial blight of rice
Publication date: Available online 20 July 2019Source: Microbiological ResearchAuthor(s): K. Sirisha, C. Ganesh Kumar, R. Hari Krishna Reddy, P. Babji, Kallaganti Venkata Siva Ramakrishna, Narendra Varma Nimmu, Cheemalamarri Chandrasekhar, Hitendra Kumar Patel, Thipparapu GanapathiAbstractThe in vitro inhibition of quorum sensing signal, xanthan gum secretion, biofilm formation in different Xanthomonas pathovars and biological control of bacterial blight of rice by the two bioactive extrolites produced by Pseudomonas aeruginosa strain CGK-KS-1 were explored. These extrolites were extracted from Diaion HP-20 resin with meth...
Source: Microbiological Research - July 21, 2019 Category: Infectious Diseases Source Type: research

Endophytic bacteria of desert cactus (Euphorbia trigonas Mill) confer drought tolerance and induce growth promotion in tomato (Solanum lycopersicum L.)
Publication date: Available online 20 July 2019Source: Microbiological ResearchAuthor(s): Pierre Eke, Aundy Kumar, Kuleshwar Prasad Sahu, Louise Nana Wakam, Neelam Sheoran, Mushineni Ashajyothi, Asharani Patel, Fabrice Boyom FekamAbstractEndophytic bacteria isolated from cactus were characterized and assessed for their capability to induce drought tolerance and growth promotion in tomato. A total of 191-bacteria representing 13-genera and 18-species were isolated from wild cactus, Euphorbia trigonas. Bacillus (58), Lysinibacillus (36), Enterobacter (29), Stenotrophomonas (18), Lelliottia (12) and Pseudomonas (12) were the ...
Source: Microbiological Research - July 21, 2019 Category: Infectious Diseases Source Type: research

Inhibition of Paenibacillus larvae by an extracellular protein fraction from a honeybee-borne Brevibacillus laterosporus strain
Publication date: Available online 19 July 2019Source: Microbiological ResearchAuthor(s): Maria Giovanna Marche, Alberto Satta, Ignazio Floris, Anna Marta Lazzeri, Luca RuiuAbstractThe inhibitory action that a Brevibacillus laterosporus strain isolated from the honeybee body causes against the American Foulbrood (AFB) etiological agent Paenibacillus larvae was studied by in-vitro experiments. A protein fraction isolated from B. laterosporus culture supernatant was involved in the observed inhibition of P. larvae vegetative growth and spore germination. As a result of LC-MS/MS proteomic analyses, the bacteriocin laterosporu...
Source: Microbiological Research - July 20, 2019 Category: Infectious Diseases Source Type: research

Engineering Bacillus velezensis with high production of acetoin primes strong induced systemic resistance in Arabidopsis thaliana
Publication date: Available online 17 July 2019Source: Microbiological ResearchAuthor(s): Ge Peng, Xiuyun Zhao, Yazhou Li, Rui Wang, Yong Huang, Gaofu QiAbstractMany plant growth promoting rhizobacteria such as Bacillus velezensis GJ11 can produce acetoin to trigger induced systemic resistance (ISR) in plants. For improving acetoin production, the mutant strains were respectively constructed by knockout of the gene of bdh (2,3-butanediol dehydrogenase) and gdh (glycerol dehydrogenase) in GJ11, but only GJ11Δbdh produced a high level of acetoin triggering strong ISR against Pseudomonas syringae infection in plants. GJ...
Source: Microbiological Research - July 18, 2019 Category: Infectious Diseases Source Type: research

A novel sRNA srvg17985 identified in Vibrio alginolyticus involving into metabolism and stress response
Publication date: Available online 17 July 2019Source: Microbiological ResearchAuthor(s): Yiqin Deng, Youlu Su, Songlin Liu, Lei Bei, Zhixun Guo, Huo Li, Chang Chen, Juan FengAbstractVibrio alginolyticus is an opportunistic pathogen that is a threat to the aquaculture industry. Evidence has revealed critical roles for small RNAs (sRNAs) in bacterial physiology and pathology by modulating gene expression post transcription. However, little information about sRNA-mediated regulation in V. alginolyticus is available. We experimentally verified the existence and characterized the function of sRNA srvg17985 in V. alginolyticus ...
Source: Microbiological Research - July 18, 2019 Category: Infectious Diseases Source Type: research

Green approach to phytopathogen: Characterization of lytic bacteriophages of Pseudomonas sp., an etiology of the bacterial blight of pomegranate
Publication date: Available online 15 July 2019Source: Microbiological ResearchAuthor(s): Smita Jagdale, Sangeeta Ahiwale, Milind Gajbhiye, Balu KapadnisAbstractTwo morphologically different bacteriophages were isolated from the river and soil samples from various locations of Maharashtra, India against the phytopathogen Pseudomonas sp. that was recently reported to cause a new bacterial blight of pomegranate. Both the phages belonged to the order Caudovirales representing the families Siphoviridae (vB_Psp.S_PRɸL2) and Myoviridae (vB_Psp.M_SSɸL8). The multiplicity of infection ranged from 0.01 to 0.1, phage adsorption ra...
Source: Microbiological Research - July 17, 2019 Category: Infectious Diseases Source Type: research

A new, rapid multiplex PCR method identifies frequent probiotic origin among clinical Saccharomyces isolates
Publication date: Available online 15 July 2019Source: Microbiological ResearchAuthor(s): Alexandra Imre, Hanna Viktória Rácz, Zsuzsa Antunovics, Zoltán Rádai, Renátó Kovács, Ksenija Lopandic, István Pócsi, Walter P. PflieglerAbstractAn increasing number of infections originating from probiotic use are reported worldwide, with the majority of such cases caused by the yeast Saccharomyces’ boulardii’, a subtype of S. cerevisiae. Reliably linking infectious cases to probiotic products requires unequivocal genotyping data, however, these techniques are ...
Source: Microbiological Research - July 17, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: September 2019Source: Microbiological Research, Volume 226Author(s): (Source: Microbiological Research)
Source: Microbiological Research - July 6, 2019 Category: Infectious Diseases Source Type: research

Hsp genes are differentially expressed during Trichoderma asperellum self-recognition, mycoparasitism and thermal stress
Publication date: Available online 2 July 2019Source: Microbiological ResearchAuthor(s): Thuana Marcolino Mota, Letícia Harumi Oshiquiri, Érica Camelo Viana Lopes, Jomal Rodrigues Barbosa Filho, Cirano José Ulhoa, Raphaela Castro GeorgAbstractHeat shock proteins (Hsp) are important factors in the response of organisms to oscillations in environmental conditions. Although Hsp have been studied for a long time, little is known about this protein class in Trichoderma species. Here we studied the expression of Hsp genes during T. asperellum growth, and mycoparasitism against two phytopathogens: Sclerotinia...
Source: Microbiological Research - July 3, 2019 Category: Infectious Diseases Source Type: research

Comparative analysis of biocontrol agent Trichoderma asperellum ACCC30536 transcriptome during its interaction with Populus davidiana × P. alba var. pyramidalis
Publication date: Available online 19 June 2019Source: Microbiological ResearchAuthor(s): Shida Ji, Zhihua Liu, Bin Liu, Yucheng Wang (Source: Microbiological Research)
Source: Microbiological Research - June 19, 2019 Category: Infectious Diseases Source Type: research