Inhibition of biofilm formation by Cd2+ on Bacillus subtilis 1JN2 depressed its biocontrol efficiency against Ralstonia wilt on tomato
Publication date: Available online 2 June 2018 Source:Microbiological Research Author(s): Wei Yang, Haixia Yan, Ji Zhang, Yuming Gao, Wei Xu, Jinshan Shang, Yuming Luo Bacillus subtilis 1JN2 can serve as an effective biocontrol agent against Ralstonia wilt on tomato, but the efficiency of control depends on the levels of heavy metals in the rhizosphere soil. Here, we investigated how the heavy metal Cd2+ affects the biocontrol efficacy of B.subtilis 1JN2 on Ralstonia wilt. We found that low Cd2+ content of 2 mM or lower had no effects on the biofilm formation of 1JN2, while media containing 3 mM or higher Cd2+ levels ...
Source: Microbiological Research - June 2, 2018 Category: Infectious Diseases Source Type: research

Growth stage and tissue specific colonization of endophytic bacteria having plant growth promoting traits in hybrid and composite maize (Zea mays L.)
In this study endophytic bacteria were isolated from a hybrid PEEHM-5 and composite PC-4 maize varieties using root, stem and leaf tissues of plants at vegetative, flowering and maturity stages of growth. PEEHM-5 harbored higher endophytic bacterial population than PC-4 at all growth stages, with highest in roots and at flowering stage. Morphologically 188 different endophytic isolates (82 from PEEHM-5, 106 from PC-4) were screened for plant growth promoting attributes viz. P, K, Zn solubilization, production of hormones, siderophore, ACC deaminase, HCN, biological nitrogen fixation and biocontrol of two maize fungal patho...
Source: Microbiological Research - May 26, 2018 Category: Infectious Diseases Source Type: research

Effectiveness of tailocins produced by Pseudomonas fluorescens SF4c in controlling the bacterial-spot disease in tomatoes caused by Xanthomonas vesicatoria
Publication date: July–August 2018 Source:Microbiological Research, Volumes 212–213 Author(s): Analía Príncipe, Maricruz Fernandez, Milenka Torasso, Agustina Godino, Sonia Fischer The development of alternatives for the use of chemical pesticides for plant disease control is the present-day and ongoing challenge for achieving sustainable agriculture. Pseudomonas fluorescens SF4c, native strain from wheat, produces tailocins (phage-tail-like bacteriocins) with antimicrobial activity against several phytopathogenic strains. We thus investigated the efficacy of foliar application of these bacterioci...
Source: Microbiological Research - May 24, 2018 Category: Infectious Diseases Source Type: research

Novel Trichoderma strains. isolated from tree barks as potential biocontrol agents and biofertilizers for direct seeded rice
This study is the first time report of utilization of Trichoderma spp. isolated from different tree barks from Odisha state of India for rice crop health management and higher productivity. Six isolates of Trichoderma spp. were identified based on the morphological characteristics and species determination was performed by molecular assays. One of the isolated strains determined as Trichoderma erinaceum outperformed others. Trichoderma erinaceum controlled three soil borne plant pathogens i.e. Rhizoctonia solani, Sclerotium rolfsii and Sclerotium oryzae effectively under controlled condition and R. solani and Helminthospor...
Source: Microbiological Research - May 23, 2018 Category: Infectious Diseases Source Type: research

Implication of orphan histidine kinase (OhkAsp) in biosynthesis of doxorubicin and daunorubicin in Streptomyces peucetius ATCC 27952
Publication date: September 2018 Source:Microbiological Research, Volume 214 Author(s): Anaya Raj Pokhrel, Hue Thi Nguyen, Dipesh Dhakal, Amit Kumar Chaudhary, Jae Kyung Sohng The orphan histidine kinase (HK) from Streptomyces peucetius ATCC 27952 (ohkAsp) was found to be implicated in the regulation of doxorubicin (DOX)/daunorubicin (DNR) biosynthesis, self-defense and developmental attributes. OhkAsp is a homolog of OhkA from Streptomyces coelicolor and Streptomyces avermitilis (with 73 and 75% identity). As in its homologs, S. peucetius mutant with deletion of ohkAsp was found to enhance metabolite biosynthesis and imp...
Source: Microbiological Research - May 23, 2018 Category: Infectious Diseases Source Type: research

Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max)
Publication date: September 2018 Source:Microbiological Research, Volume 214 Author(s): Yuan Hui Liu, En Tao Wang, Yin Shan Jiao, Chang Fu Tian, Lei Wang, Zi Jian Wang, Jia Jing Guan, Raghvendra Pratap Singh, Wen Xin Chen, Wen Feng Chen Site-specific insertion plasmid pVO155 was used to knockout the genes involved in the alternation of host range of strain Bradyrhizobium diazoefficiens USDA 110 from its original determinate-nodule-forming host soybean (Glycine max), to promiscuous and indeterminate-nodule-forming shrubby legume sophora (Sophora flavescens). Symbiotic phenotypes of these mutants inoculated to these two leg...
Source: Microbiological Research - May 21, 2018 Category: Infectious Diseases Source Type: research

LotS/LotR/Clp, a novel signal pathway responding to temperature, modulating protease expression via c-di-GMP mediated manner in Stenotrophomonas maltophilia FF11
Publication date: Available online 19 May 2018 Source:Microbiological Research Author(s): Qingling Wang, Fangling Ji, Jianli Guo, Yuepeng Wang, Yanyan Li, Jingyun Wang, Lijia An, Yongming Bao Stenotrophomonas maltophilia as one of increasing food spoilage bacteria and fish pathogens has become a threat to aquiculture industry. A major factor contributing to the success of bacterium is its outstanding ability to secrete protease at low temperatures. Here, a cAMP receptor like protein (Clp) shows a positive regulation on this protease, named S. maltophilia temperature-response protease (SmtP). Interestingly, a two-component...
Source: Microbiological Research - May 19, 2018 Category: Infectious Diseases Source Type: research

The pyrophosphohydrolase RppH is involved in the control of RsmA/CsrA expression in Azotobacter vinelandii and Escherichia coli
This study shows that in the soil bacterium Azotobacter vinelandii, inactivation of rppH gene negatively affected the production of bioplastic poly-β-hydroxybutyrate (PHB) by reducing the expression at the translational level of PhbR, the specific transcriptional activator of the phbBAC biosynthetic operon. The effect of RppH on the translation of phbR seemed to be exerted through the translational repressor RsmA, as the inactivation of rsmA in the rppH mutant restored the phbR expression. Interestingly, in Escherichia coli inactivation of rppH also affected the expression of CsrA, the RsmA homolog. The level of the c...
Source: Microbiological Research - May 18, 2018 Category: Infectious Diseases Source Type: research

Hydroxycoumarins: New, effective plant-derived compounds reduce Ralstonia pseudosolanacearum populations and control tobacco bacterial wilt
Publication date: Available online 17 May 2018 Source:Microbiological Research Author(s): Liang Yang, Lintong Wu, Xiaoyuan Yao, Shiyuan Zhao, Jiao Wang, Shili Li, Wei Ding Plant wilt disease caused by the soilborne bacterial pathogen Ralstonia pseudosolanacearum is one of the most devastating plant diseases; however, no effective protection against this disease has been developed. Coumarins are important natural plant-derived compounds with a wide range of bioactivities and extensive applications in medicine and agriculture. In the present study, three hydroxycoumarins (Hycs), umbelliferone (UM), esculetin (ES) and daphne...
Source: Microbiological Research - May 17, 2018 Category: Infectious Diseases Source Type: research

Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro
Publication date: Available online 13 May 2018 Source:Microbiological Research Author(s): Renu Verma, Thaís Cabrera Galvão Rojas, Renato Pariz Maluta, Janaína Luisa Leite, Gerson Nakazato, Wanderley Dias de Silveira Avian Pathogenic Escherichia coli (APEC) strains belong to the extra-intestinal pathogenic group of E. coli (ExPEC) that causes colibacillosis in poultry. A variety of putative virulence factors of APEC are recognized as potent causes of pathogenicity, the mechanisms underlying their pathogenicity are still not fully understood. The role of yicS in the virulence of pathogenic E. coli is st...
Source: Microbiological Research - May 13, 2018 Category: Infectious Diseases Source Type: research

Community structure and plant growth-promoting potential of cultivable bacteria isolated from Cameroon soil
Publication date: Available online 12 May 2018 Source:Microbiological Research Author(s): Gylaine Vanissa Tchuisseu Tchakounté, Beatrice Berger, Sascha Patz, Fankem Henri, Silke Ruppel Exploiting native plant growth-promoting rhizobacteria (PGPR) in Cameroonian agro-ecosystems provides a means to improve plant–microbe interactions that may enhance ecosystem sustainability and agricultural productivity in an environmentally eco-friendly way. Consequently, we aimed to investigate the community structure and functional PGPR diversity of maize grown in Cameroon. Native bacteria isolated from Cameroon maize rhizos...
Source: Microbiological Research - May 12, 2018 Category: Infectious Diseases Source Type: research

Effectiveness of multi-trait Burkholderia contaminans KNU17BI1 in growth promotion and management of banded leaf and sheath blight in maize seedling
This study for the first time demonstrated potent in vitro antifungal activity of B. contaminans against Rhizoctonia solani AG-1(IA), Pythium graminicola, Fusarium moniliforme, Alternaria alternata, Alternaria solani, Fusarium graminearum, Stemphylium botryosum Wallr, Colletotrichum dematium, Stemphylium lycopersici and Fusarium oxysporum f.sp. melonis. Furthermore, in this study, for the first time, the potential of B. contaminans stain KNU17BI1 in controlling banded leaf and sheath blight of maize caused by R. solani AG-1(IA) was reported. Therefore, further studies are warranted on the structural identification of actua...
Source: Microbiological Research - May 6, 2018 Category: Infectious Diseases Source Type: research

Signature-tagged mutagenesis screening revealed the role of lipopolysaccharide biosynthesis gene rfbH in smooth-to-rough transition in Salmonella Enteritidis
In this study, we used monoclonal antibody against O-antigens (O9 mAb) to identify novel factors that are involved in LPS synthesis and rough variation in S. Enteritidis by using signature-tagged mutagenesis (STM) technique. Our results showed that transposon insertion in the gene rfbH led to different LPS phenotype, auto-aggregation characteristic, motility and resistance to environmental stress compared with SE wild-type strain C50041. In addition, sera tests showed that rfbH mutant does not elicit specific antibodies against O-antigens in vaccinated animals. Taken together, the S. Enteritidis rfbH gene is implicated in ...
Source: Microbiological Research - May 5, 2018 Category: Infectious Diseases Source Type: research

Master mechanisms of Staphylococcus aureus: consider its excellent protective mechanisms hindering vaccine development
Publication date: Available online 4 May 2018 Source:Microbiological Research Author(s): Xu Zhang, Manukumar H. Marichannegowda, Kadalipura P. Rakesh, Hua-Li Qin Lack of known mechanisms of protection against Staphylococcus aureus in humans represents an important risk factor for skin infections and bacteremia in patients, intern hindering the development of efficacious vaccines. However, development of effective humoral response may be dampened by converging immune-evasion mechanisms of S. aureus. To develop a promising vaccine against S. aureus, it is pre-requisite to clear understanding of cutaneous, innate and adaptiv...
Source: Microbiological Research - May 4, 2018 Category: Infectious Diseases Source Type: research

Brazilian red propolis: Chemical composition and antibacterial activity determined using bioguided fractionation
Publication date: Available online 4 May 2018 Source:Microbiological Research Author(s): Luciane Corbellini Rufatto, Paola Luchtenberg, Charlene Garcia, Christine Thomassigny, Sylvie Bouttier, Françoise Dumas, João Antonio Pêgas Henriques, Mariana Roesch-Ely, Sidnei Moura The indiscriminate use of antibiotics is causing an increase in bacterial resistance, complicating therapeutic planning. In this context, natural products have emerged as major providers of bioactive compounds. This work performs a bioguided study of Brazilian red propolis to identify compounds with antibacterial potential and to eval...
Source: Microbiological Research - May 4, 2018 Category: Infectious Diseases Source Type: research

The developmental regulator Pcz1 affects the production of secondary metabolites in the filamentous fungus Penicillium roqueforti
Publication date: Available online 4 May 2018 Source:Microbiological Research Author(s): Juan F. Rojas-Aedo, Carlos Gil-Durán, Alejandra Goity, Inmaculada Vaca, Gloria Levicán, Luis F. Larrondo, Renato Chávez Penicillium roqueforti is used in the production of several kinds of ripened blue-veined cheeses. In addition, this fungus produces interesting secondary metabolites such as roquefortine C, andrastin A and mycophenolic acid. To date, there is scarce information concerning the regulation of the production of these secondary metabolites. Recently, the gene named pcz1 (Penicillium C6 zinc domain pro...
Source: Microbiological Research - May 4, 2018 Category: Infectious Diseases Source Type: research

Clioquinol induces G2/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae
Publication date: Available online 4 May 2018 Source:Microbiological Research Author(s): Chongjia Yan, Song Wang, Jian Wang, Hui Li, Zhiwei Huang, Jing Sun, Min Peng, Wenbin Liu, Ping Shi Clioquinol (CQ) has been used as a classical antimicrobial agent for many years. However, its mode of action is still unclear. In our study, the growth of Candida albicans and Saccharomyces cerevisiae was inhibited by CQ. It did not kill yeast cells, but shortened G1 phase and arrested cell cycle at G2/M phase. By using two-dimensional electrophoresis based proteomic approach, six proteins were found to be significantly affected by CQ. A...
Source: Microbiological Research - May 4, 2018 Category: Infectious Diseases Source Type: research

Pathogenesis-Related Proteins and Peptides as Promising Tools for Engineering Plants with Multiple Stress Tolerance
Publication date: Available online 30 April 2018 Source:Microbiological Research Author(s): Sajad Ali, Bashir Ahmad Ganai, Azra N Kamili, Ajaz Ali Bhat, Zahoor Ahmad Mir, Javaid Akhter Bhat, Anshika Tyagi, Sheikh Tajamul Islam, Muntazir Mushtaq, Prashant Yadav, Sandhya Rawat, Anita Grover Pathogenesis-related (PR) proteins and antimicrobial peptides (AMPs) are a group of diverse molecules that are induced by phytopathogens as well as defense related signaling molecules. They are the key components of plant innate immune system especially systemic acquired resistance (SAR), and are widely used as diagnostic molecular marke...
Source: Microbiological Research - April 30, 2018 Category: Infectious Diseases Source Type: research

Evidence for Up and Down Regulation of 450 genes by rpoB12 (rif) Mutation and their Implications in Complexity of Transcription Modulation in Escherichia coli
Publication date: Available online 30 April 2018 Source:Microbiological Research Author(s): Shanmugaraja Meenakshi, M. Hussain Munavar Analyses of mutations in rpoB subunit of Escherichia coli that lead to resistance to rifampicin have been invaluable in providing insight into events during transcription continue to be discovered. Earlier we reported that rpoB12 suppresses over-expression of cps genes in Δlon mutant of E.coli, by interfering with the transcription of rcsA. Here we report Microarray based Transcriptome profile of Δlon and Δlon rpoB12 strains. The data analyses clearly reveal that rpoB12 m...
Source: Microbiological Research - April 30, 2018 Category: Infectious Diseases Source Type: research

Biotechnological applications of bacteriophages: State of the art
Publication date: Available online 30 April 2018 Source:Microbiological Research Author(s): Liliam K. Harada, Erica C. Silva, Welida F. Campos, Fernando S. Del Fiol, Marta Vila, Krystyna Dąbrowska, Victor N. Krylov, Victor M. Balcão Bacteriophage particles are the most abundant biological entities on our planet, infecting specific bacterial hosts in every known environment and being major drivers of bacterial adaptive evolution. The study of bacteriophage particles potentially sheds light on the development of new biotechnology products. Bacteriophage therapy, although not new, makes use of strictly lytic phage pa...
Source: Microbiological Research - April 30, 2018 Category: Infectious Diseases Source Type: research

Chemical interaction of endophytic fungi and actinobacteria from Lychnophora ericoides in co-cultures
Publication date: Available online 24 April 2018 Source:Microbiological Research Author(s): Fernanda O. Chagas, Mônica T. Pupo Microorganisms interact chemically in natural environments; however, the compounds and mechanisms involved in this phenomenon are still poorly understood. Using the cocultivation approach, changes in metabolic profiles due to interactions between endophytic fungal and actinobacterial strains isolated from the plant Lychnophora ericoides (Asteraceae) were assessed. The production of the cytotoxic compound cytochalasin H by the fungus Phomopsis sp. FLe6 was remarkably inhibited in solid and li...
Source: Microbiological Research - April 25, 2018 Category: Infectious Diseases Source Type: research

Retraction notice to Marine actinobacterial metabolites: current status and future perspectives MICRES_168_6(2013)311-322
Publication date: June 2018 Source:Microbiological Research, Volume 211 Author(s): Panchanathan Manivasagan, Jayachandran Venkatesan, Kannan Sivakumar, Se-Kwon Kim (Source: Microbiological Research)
Source: Microbiological Research - April 25, 2018 Category: Infectious Diseases Source Type: research

Bacterial endophytes modulates the withanolide biosynthetic pathway and physiological performance in Withania somnifera under biotic stress
Publication date: Available online 22 April 2018 Source:Microbiological Research Author(s): Aradhana Mishra, Satyendra Pratap Singh, Sahil Mahfooz, Arpita Bhattacharya, Nishtha Mishra, Pramod Arvind Shirke, C.S. Nautiyal Despite the vast exploration of endophytic microbes for growth enhancement in various crops, knowledge about their impact on the production of therapeutically important secondary metabolites is scarce. In the current investigation, chitinolytic bacterial endophytes were isolated from selected medicinal plants and assessed for their mycolytic as well as plant growth promoting potentials. Among them the two...
Source: Microbiological Research - April 23, 2018 Category: Infectious Diseases Source Type: research

Talaromyces pinophilus strain AUN-1 as a novel mycoparasite of Botrytis cinerea, the pathogen of onion scape and umbel blights
In conclusion, this study demonstrated that the endophytic T. pinophilus may be a promising biocontrol agent against phytopathogenic fungi instead of chemical fungicides. Graphical abstract (Source: Microbiological Research)
Source: Microbiological Research - April 19, 2018 Category: Infectious Diseases Source Type: research

Comparison of genomic islands in cyanobacteria: evidence of bacteriophage-mediated horizontal gene transfer from eukaryotes
Publication date: Available online 11 April 2018 Source:Microbiological Research Author(s): James S. Godde, Shakuntala Baichoo, Zahra Mungloo-Dilmohamud, Yasmina Jaufeerally-Fakim A number of examples of putative eukaryote-to-prokaryote horizontal gene transfer (HGT) have been proposed in the past using phylogenetic analysis in support of these claims but none have attempted to map these gene transfers to the presence of genomic islands (GIs) in the host. Two of these cases have been examined in detail, including an ATP sulfurylase (ATPS) gene and a class I fructose bisphosphate aldolase (FBA I) gene that were putatively ...
Source: Microbiological Research - April 12, 2018 Category: Infectious Diseases Source Type: research

Tissue age and plant genotype affect the microbiota of apple and pear bark
Publication date: Available online 10 April 2018 Source:Microbiological Research Author(s): Elena Arrigoni, Livio Antonielli, Massimo Pindo, Ilaria Pertot, Michele Perazzolli Plant tissues host complex fungal and bacterial communities, and their composition is determined by host traits such as tissue age, plant genotype and environmental conditions. Despite the importance of bark as a possible reservoir of plant pathogenic microorganisms, little is known about the associated microbial communities. In this work, we evaluated the composition of fungal and bacterial communities in the pear (Abate and Williams cultivars) and ...
Source: Microbiological Research - April 11, 2018 Category: Infectious Diseases Source Type: research

Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains
Publication date: Available online 7 April 2018 Source:Microbiological Research Author(s): Daniela C. Sabaté, Carolina Pérez Brandan, Gabriela Petroselli, Rosa Erra-Balsells, M.Carina Audisio Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root ...
Source: Microbiological Research - April 8, 2018 Category: Infectious Diseases Source Type: research

Phenotypic and genotypic characterisation of an unique indigenous hypersaline unicellular cyanobacterium, Euhalothece sp.nov
Publication date: Available online 3 April 2018 Source:Microbiological Research Author(s): Trisha Mogany, Feroz M. Swalaha, Mushal Allam, Phillip Senzo Mtshali, Arshad Ismail, Sheena Kumari, Faizal Bux A novel halotolerant species of cyanobacterium of the order Chroococcales was isolated from hypersaline estuary in Kwa-Zulu Natal, South Africa. A comprehensive polyphasic approach viz., cell morphology, pigment composition and complete genome sequence analysis was conducted to elucidate the taxonomic position of the isolated strain. The blue-green oval to rod-shaped cells were 14–18 μm in size, and contained a h...
Source: Microbiological Research - April 4, 2018 Category: Infectious Diseases Source Type: research

Antibiotic-producing Pseudomonas fluorescens mediates Rhizome Rot Disease resistance and promotes plant growth in Turmeric plants
This study intends to evaluate the performance of fluorescent pseudomonads against Rhizome rot disease and understand the resistance mechanism in Turmeric plants. Fluorescent pseudomonads were screened against Pythium aphanidermatum using dual culture. Selected strains were evaluated for the performance of growth promoting attributes and the presence of antibiotic genes through PCR analysis. Strain FP7 recorded the maximum percent inhibition of P. aphanidermatum under in vitro conditions. Strains FP7 and TPF54 both increased plant growth in turmeric plants in vitro. Strain FP7 alone contained all the evaluated antibiotic b...
Source: Microbiological Research - March 27, 2018 Category: Infectious Diseases Source Type: research

Subterranean infestation by Holotrichia parallela larvae is associated with changes in the peanut (Arachis hypogaea L.) rhizosphere microbiome
This study provides the insight into the function of rhizosphere microbiome in the interaction between subterranean pests and crops. (Source: Microbiological Research)
Source: Microbiological Research - March 24, 2018 Category: Infectious Diseases Source Type: research

Quorum sensing: A less known mode of communication among fungi
Publication date: Available online 21 March 2018 Source:Microbiological Research Author(s): Sajad Ahmad Padder, Rajendra Prasad, Abdul Haseeb Shah Quorum sensing (QS), a density-dependent signaling mechanism of microbial cells, involves an exchange and sense of low molecular weight signaling compounds called autoinducers. With the increase in population density, the autoinducers accumulate in the extracellular environment and once their concentration reaches a threshold, many genes are either expressed or repressed. This cell density-dependent signaling mechanism enables single cells to behave as multicellular organisms a...
Source: Microbiological Research - March 22, 2018 Category: Infectious Diseases Source Type: research

Fungal communities associated with Evernia prunastri, Ramalina fastigiata and Pleurosticta acetabulum: three epiphytic lichens potentially active against Candida biofilms
This study shows that common lichens are reservoirs of diverse fungal communities, which could potentially contribute to global activity of the lichen and, therefore, deserve to be isolated for further chemical studies. (Source: Microbiological Research)
Source: Microbiological Research - March 22, 2018 Category: Infectious Diseases Source Type: research

Inhibition of coxsackievirus B4 by Lactobacillus plantarum
Publication date: Available online 19 March 2018 Source:Microbiological Research Author(s): Mattia Pia Arena, Firas Elmastour, Famara Sane, Djamel Drider, Daniela Fiocco, Giuseppe Spano, Didier Hober The enterovirus Coxsackievirus B4 (CV-B4) can infect different human tissues and provoke abnormal function or destruction of various organs and cells. Moreover, its infections have been linked to the onset of type 1 diabetes. Coxsackievirus B4 is classified as a “challenging virus”, due to the intense yet vain efforts to find effective prevention and therapeutic agents, especially within biological compounds. Lact...
Source: Microbiological Research - March 20, 2018 Category: Infectious Diseases Source Type: research

Population diversity of bacterial endophytes from jute (Corchorus olitorius) and evaluation of their potential role as bioinoculants
This study was conducted to explore the potential of jute bacterial endophytes for their growth promotion ability in direct and indirect ways. A total of 27 different bacterial species were identified from different varieties of a jute plant (Corchorus olitorius) and different parts of the plant (leaf, root, seed, and seedling) based on 16S rRNA gene sequence. Two of the isolates showed ACC deaminase activity with Staphylococcus pasteuri strain MBL_B3 and Ralstonia solanacearum strain MBL_B6 producing 18.1 and 8.08 μM mg−1 h−1 α-ketobutyrate respectively while eighteen had the ACC deaminase gene ...
Source: Microbiological Research - March 16, 2018 Category: Infectious Diseases Source Type: research

Multicellular behavior of environmental Escherichia coli isolates grown under nutrient-poor and low-temperature conditions
This study documents the ability of Escherichia cryptic clades, which have recently been recognized as new lineages genetically divergent from E. coli, to grow in unfavorable conditions through expression of distinct phenotypes. Growth under low-temperature and nutrient-poor conditions induced the rdar morphotype in all cryptic clade strains tested, especially after preincubation in broth supplemented with uracil. Such phenotypic response to harsh growth conditions was clearly detected by transmission and scanning electron microscopy, which showed that bacteria were encased in a fibrous matrix. Conversely, cells incubated ...
Source: Microbiological Research - March 16, 2018 Category: Infectious Diseases Source Type: research

Roles of phospholipid methyltransferases in pycnidia development, stress tolerance and secondary metabolism in the taxol-producing fungus Pestalotiopsis microspore
We report here the characterization of two genes, choA and choC, that putatively encoded phospholipid methyltransferases in the taxol-producing fungus Pestalotiopsis microspora. Deletion of choC resulted in defects in PC production, vegetative growth and development of asexual structure. The mutant strains exhibited multiple morphological abnormalities, e.g. swollen hyphal tips and enhanced hyphal branching, and even mycelial autolysis. Some novel roles for the genes were also revealed, for instance, the deletion of either choC or choA impaired the development of pycnidia and conidia, the cell wall integrity. The mutant st...
Source: Microbiological Research - March 9, 2018 Category: Infectious Diseases Source Type: research

Characterization of Cd-resistant Klebsiella michiganensis MCC3089 and its potential for rice seedling growth promotion under Cd stress
Publication date: Available online 8 March 2018 Source:Microbiological Research Author(s): Soumik Mitra, Krishnendu Pramanik, Pallab Ghosh, Tithi Soren, Anumita Sarkar, Ramendra Sundar Dey, Sanjeev Pandey, Tushar Kanti Maiti Application of heavy metal resistant plant growth promoting rhizobacteria has an important role as they help to evade metal-induced toxicity in plants on one hand and enhance plant growth on the other. The present study is therefore focused on the characterization of a cadmium resistant bacterial strain isolated from heavy metal contaminated rhizospheric soil designated as S8. This S8 strain was selec...
Source: Microbiological Research - March 9, 2018 Category: Infectious Diseases Source Type: research

The transcription factor-encoding gene crtf is involved in Clonostachys chloroleuca mycoparasitism on Sclerotinia sclerotiorum
In this study, the function of crtf during 67-1 mycoparasitism was verified through gene knockout and complementation. The results showed that deletion of crtf did not influence fungal morphological characteristics, but the ability of the Δcrtf mutant to parasitize sclerotia and suppress soybean Sclerotinia white mold in the greenhouse was markedly diminished compared with the wild type strain. The biocontrol activity of Δcrtf recovered wild type levels when complemented with a plasmid expressing the crtf gene. These findings suggest that crtf plays a crucial role in C. chloroleuca mycoparasitism and provide in...
Source: Microbiological Research - March 8, 2018 Category: Infectious Diseases Source Type: research

The role of efflux pumps in Bacteroides fragilis resistance to antibiotics
Publication date: Available online 1 March 2018 Source:Microbiological Research Author(s): Reza Ghotaslou, Mina Yekani, Mohammad Yousef Memar The resistance of Bacteroides fragilis to the most antimicrobial agents has been reported in the world. Identification of the microbial resistance mechanisms can play an important role in controlling these resistances. Currently, B. fragilis is resistant to most antibiotics. The multi-drug efflux pumps have been shown to underlie the antimicrobial resistance in B. fragilis strains. Two types of these efflux pumps including RND and MATE can be regarded as main structures responsible ...
Source: Microbiological Research - March 2, 2018 Category: Infectious Diseases Source Type: research

Phosphorylation of PppA at threonine 253 controls T6SS2 expression and bacterial killing capacity in the marine pathogen Vibrio alginolyticus
Publication date: Available online 22 February 2018 Source:Microbiological Research Author(s): Zhen Yang, Xuetong Wang, Wensheng Xu, Mian Zhou, Yuanxing Zhang, Yue Ma, Qiyao Wang Type VI secretion systems (T6SSs) are multi-protein secretory nano-machines that mediate inter-bacterial competition. Vibrio alginolyticus is an abundant gram-negative marine bacterium that efficiently kills other bacteria with its T6SS2. The V. alginolyticus T6SS2 gene cluster encodes a phosphatase, PppA, and a type II membrane-spanning Hanks-type threonine kinase, PpkA2, which have been implicated in the activation of T6S. Meanwhile, T6SS2 gene...
Source: Microbiological Research - February 23, 2018 Category: Infectious Diseases Source Type: research

Heat stress-induced reactive oxygen species participate in the regulation of HSP expression, hyphal branching and ganoderic acid biosynthesis in Ganoderma lucidum
In this study, we found that HS induced a significant increase in the cytosolic ROS concentration, and exogenously added ROS scavengers NAC, VC and NADPH oxidase (Nox) inhibitor DPI reduce the cytosolic ROS accumulation in G. lucidum. In addition, the phenomena of the increased gene expression and increased length between the two hyphal branches and the accumulation of GA biosynthesis induced by HS were mitigated. Furthermore, we investigated the effects of HS on Nox-silenced strains (NoxABi-10, NoxABi-11 and NoxRi-4, NoxRi-7) and found that the level of ROS concentration was lower than that in wild-type (WT) strains treat...
Source: Microbiological Research - February 20, 2018 Category: Infectious Diseases Source Type: research

Antidiabetic “gliptins” affect biofilm formation by Streptococcus mutans
Publication date: Available online 19 February 2018 Source:Microbiological Research Author(s): Arpan De, Arianna Pompilio, Jenifer Francis, Iain C. Sutcliffe, Gary W. Black, Giulio Lupidi, Dezemona Petrelli, Luca A. Vitali Streptococcus mutans, a dental caries causing odontopathogen, produces X-prolyl dipeptidyl peptidase (Sm-XPDAP, encoded by pepX), a serine protease known to have a nutritional role. Considering the potential of proteases as therapeutic targets in pathogens, this study was primarily aimed at investigating the role of Sm-XPDAP in contributing to virulence-related traits. Dipeptidyl peptidase (DPP IV), an ...
Source: Microbiological Research - February 20, 2018 Category: Infectious Diseases Source Type: research

Biocontrol activity of surfactin A purified from Bacillus NH-100 and NH-217 against rice bakanae disease
This study evaluates the biocontrol activity of surfactin-producing Bacillus (SPB) strains NH-100 and NH-217 and purified surfactin A from these strains against rice bakanae disease. Biologically active surfactin fractions were purified by HPLC, and surfactin A variants with chain lengths from C12 to C16 were confirmed by LCMS-ESI. In hemolytic assays, a positive correlation between surfactin A production and halo zone formation was observed. The purified surfactin A had strong antifungal activity against Fusarium oxysporum, F. moniliforme, F. solani, Trichoderma atroviride and T. reesei. Maximum fungal growth suppression ...
Source: Microbiological Research - February 16, 2018 Category: Infectious Diseases Source Type: research

Plant Growth Promoting Bacteria as an Alternative Strategy for Salt Tolerance in Plants: A Review
Publication date: Available online 13 February 2018 Source:Microbiological Research Author(s): Muhammad Numan, Samina Bashir, Yasmin Khan, Roqayya Mumtaz, Zabta Khan Shinwari, Abdul Latif Khan, Ajmal Khan, Ahmed AL-Harrasi Approximately 5.2 billion hectare agriculture land are affected by erosion, salinity and soil degradation. Salinity stress has significantly affecting the fertile lands, and therefore possesses a huge impact on the agriculture and economy of a country. Salt stress has severe effects on the growth and development of plants as well as reducing its yield. Plants are inherently equipped with stress toleranc...
Source: Microbiological Research - February 15, 2018 Category: Infectious Diseases Source Type: research

Physiologically distinct subpopulations formed in Escherichia coli cultures in response to heat shock
Publication date: Available online 13 February 2018 Source:Microbiological Research Author(s): Bożena Bruhn-Olszewska, Paweł Szczepaniak, Ewelina Matuszewska, Dorota Kuczyńska-Wiśnik, Karolina Stojowska-Swędrzyńska, María Moruno Algara, Ewa Laskowska Bacteria can form heterogeneous populations containing phenotypic variants of genetically identical cells. The heterogeneity of populations can be considered a bet-hedging strategy allowing adaptation to unknown environmental changes – at least some individual subpopulations or cells might be able to withstand future adverse conditions. Using Percoll gradie...
Source: Microbiological Research - February 14, 2018 Category: Infectious Diseases Source Type: research

Transcriptional control of the phenol hydroxylase gene phe of Corynebacterium glutamicum by the AraC-type regulator PheR
Publication date: Available online 6 February 2018 Source:Microbiological Research Author(s): Can Chen, Yaoling Zhang, Lei Xu, Kaixiang Zhu, Yanyan Feng, Junfeng Pan, Meiru Si, Lei Zhang, Xihui Shen Corynebacterium glutamicum can degrade phenol by a meta-cleavage pathway, which depends on ncgl2588 (phe) of the phe operon encoding phenol hydroxylase. An additional gene, ncgl2587 (pheR), is located upstream of phe. The pheR encodes an AraC/XylR-type regulator protein with 377 amino acid residues and is transcribed in the same direction as phe. Disruption of pheR by homologous recombination resulteds in the accumulation of p...
Source: Microbiological Research - February 7, 2018 Category: Infectious Diseases Source Type: research

Gibberellin biosynthesis and metabolism: a convergent route for plants, fungi and bacteria
Publication date: Available online 3 February 2018 Source:Microbiological Research Author(s): Sonia Salazar-Cerezo, Nancy Martínez-Montiel, Jenny García-Sánchez, Rocío Pérez-y-Terrón, Rebeca D. Martínez-Contreras Gibberellins (GAs) are natural complex biomolecules initially identified as secondary metabolites in the fungus Gibberella fujikuroi with strong implications in plant physiology. GAs have been identified in different fungal and bacterial species, in some cases related to virulence, but the full understanding of the role of these metabolites in the different organis...
Source: Microbiological Research - February 3, 2018 Category: Infectious Diseases Source Type: research

Identification and characterization of a new Bacillus atrophaeus strain B5 as biocontrol agent of postharvest anthracnose disease in soursop (Annona muricata) and avocado (Persea americana)
In this study, we evaluated the activity of metabolites contained in the cell free supernatant, obtained from Bacillus strain B5 culture, against micelial growth and spore germination of two virulent strains of C. gloeosporioides isolated from soursop and avocado. On the basis of 16S rDNA gene sequence analysis, this strain was identified as Bacillus atrophaeus. A preventive treatment using cell free supernatant, reduced severity and incidence of anthracnose disease on harvested soursop and avocado fruit. B. atrophaeus strain B5 harbors genes involved in the production of antibiotics such as surfactin, bacillomycin and itu...
Source: Microbiological Research - February 2, 2018 Category: Infectious Diseases Source Type: research

Marine microbial L-asparaginase: biochemistry, molecular approaches and applications in tumor therapy and in food industry
Publication date: Available online 2 February 2018 Source:Microbiological Research Author(s): Fatemeh Izadpanah, Ahmad Homaei, Pedro Fernandes, Sedigheh Javadpour The marine environment is a rich source of biological and chemical diversity. It covers more than 70% of the Earth’s surface and features a wide diversity of habitats, often displaying extreme conditions, where marine organisms thrive, offering a vast pool for microorganisms and enzymes. Given the dissimilarity between marine and terrestrial habitats, enzymes and microorganisms, either novel or with different and appealing features as compared to terrestri...
Source: Microbiological Research - February 2, 2018 Category: Infectious Diseases Source Type: research

Antagonistic Bacillus spp. reduce blast incidence on rice and increase grain yield under field conditions —Bio control effects on blast disease suppression on rice crop
In this study, Bacillus spp. KFP-5, KFP-7, KFP-17 significantly reduced disease severity by 40–52% with grain yield of 3.2–3.9 ton ha−1 in two rice varieties i.e., basmati super and basmati 385. Bacillus spp. significantly colonized the rice rhizosphere with a cell population of 2.40E+06–5.6E+07CFU. Rice plants treated with antagonistic bacterial suspension followed by challenge inoculation with P. oryzae were found to have higher activities of antioxidant enzymes such as superoxide dismutase (308–266 Ug−1 FW), peroxidase (change in absorbance (ΔA) = 0.20–0.71 m...
Source: Microbiological Research - January 31, 2018 Category: Infectious Diseases Source Type: research