Matricaria genus as a source of antimicrobial agents: From farm to pharmacy and food applications
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Mehdi Sharifi-Rad, Jolanta Nazaruk, Letizia Polito, Maria Flaviana Bezerra Morais-Braga, Janaína Esmeraldo Rocha, Henrique Douglas Melo Coutinho, Bahare Salehi, Giulia Tabanelli, Chiara Montanari, María del Mar Contreras, Zubaida Yousaf, William N. Setzer, Deepa R. Verma, Miquel Martorell, Antoni Sureda, Javad Sharifi-RadAbstractMatricaria is a widespread genus of flowering plants of the family Asteraceae that grow in temperate regions of Europe, Asia, America and Africa. Some of the species are also naturalized in Australia....
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Antibiotic producing endophytic Streptomyces spp. colonize above-ground plant parts and promote shoot growth in multiple healthy and pathogen-challenged cereal crops
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Janki K. Patel, Sheeba Madaan, G. ArchanaAbstractThe Streptomyces spp. used in this work were previously isolated as diazotrophic endophytes from sorghum stems. Here, we characterized the Streptomyces spp. for their colonization ability, plant growth promotion and protection against fungal disease in three cereals. In vitro analysis by dual culture study showed inhibitory effect on the rice pathogen Magnaporthe oryzae B157 along with inhibition of the ubiquitous phytopathogen Rhizoctonia solani by the Streptomyces spp. used in this study. ...
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): How Lie Ling, Zaidah Rahmat, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, Rosli Md. IlliasAbstractBacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding of bacterial acclimation to alkaline media through analyzing extracellular proteins of B. lehensis. The pH range for B. lehensis growth was conduc...
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Pyrrolnitrin is more essential than phenazines for Pseudomonas chlororaphis G05 in its suppression of Fusarium graminearum
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Run Huang, Zhibin Feng, Xiaoyan Chi, Xiaoqiang Sun, Yang Lu, Baoshen Zhang, Ruiyang Lu, Wangtai Luo, Yanhua Wang, Jing Miao, Yihe GeAbstractFusarium graminearum is the major causal agent of Fusarium head blight (FHB) disease in cereal crops worldwide. Infection with this fungal phytopathogen can regularly cause severe yield and quality losses and mycotoxin contamination in grains. In previous other studies, one research group reported that pyrrolnitrin had an ability to suppress of mycelial growth of F. graminearum. Other groups revealed t...
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Designer probiotic Lactobacillus plantarum expressing oxalate decarboxylase developed using group II intron degrades intestinal oxalate in hyperoxaluric rats
In conclusion, the study demonstrates that food-grade L. plantarum secreting OxdC is capable of degrading intestinal oxalate and thereby prevent CaOx stone formation in experimental rats. (Source: Microbiological Research)
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Bacteria and bacterial products: Foe and friends to Caenorhabditis elegans
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Fazlurrahman Khan, Saurabh Jain, Sandra Folarin OloketuyiAbstractCaenorhabditis elegans is a model organism for the study of different molecular, biochemical, microbial and immunity-related mechanisms. In its natural habitat, C. elegans survives by feeding microorganisms (mainly bacteria), though majorly on Escherichia coli OP50 when grown in the laboratory. Numerous bacteria are shown to influence the lifespan, behavioural responses and innate immunity of C. elegans. The secondary metabolites produced by bacteria have shown to play key ro...
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Specific growth inhibitors of Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, and Clavibacter michiganensis subsp. michiganensis
In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at>5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E co...
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

'Ropy' phenotype, exopolysaccharides and metabolism: study on food isolated potential probiotics LAB
Publication date: Available online 9 July 2018Source: Microbiological ResearchAuthor(s): Simona Cirrincione, Yael Breuer, Erika Mangiapane, Roberto Mazzoli, Enrica PessioneABSTRACTLactic acid bacteria are fully recognized for their industrial applications among which the production and release of exopolysaccharides. In the present investigation, we screened fifteen Lactobacilli in order to find ropy strains, quantify exopolysaccharides and detect proteins specifically associated with the ropy-exopolysaccharide production. The highest ropy-exopolysaccharide producer (L. helveticus 6E8), was grown in stimulating and basal co...
Source: Microbiological Research - July 10, 2018 Category: Infectious Diseases Source Type: research

Role of two-component regulatory systems in the virulence of Streptococcus suis
Publication date: Available online 7 July 2018Source: Microbiological ResearchAuthor(s): Chengkun Zheng, Lingzhi Li, Haojie Ge, Hongmei Meng, Yang Li, Weicheng Bei, Xiaohui ZhouAbstractStreptococcus suis is an important zoonotic pathogen that causes severe infections and great economic losses worldwide. Understanding how this pathogen senses and responds to environmental signals during the infectious process can offer insight into its pathogenesis and may be helpful in the development of drug targets. Two-component regulatory systems (TCSs) play an essential role in this environmental response. In S. suis, at least 15 grou...
Source: Microbiological Research - July 8, 2018 Category: Infectious Diseases Source Type: research

Time-resolved transcriptome analysis of Clostridium difficile R20291 response to cysteine
Publication date: Available online 7 July 2018Source: Microbiological ResearchAuthor(s): Huawei Gu, Kan Shi, Zhengping Liao, Haonan Qi, Shuyi Chen, Haiying Wang, Shan Li, Yi Ma, Jufang WangAbstractThe incidence of Clostridium difficile infection has been steadily rising over the past decade. The increase in the rate of incidence is associated with the specific NAP1/BI/027 strains which are “hypervirulent” and have led to several large outbreaks since their emergence. However, the relation between these outbreaks and virulence regulation mechanisms remains unclear. It has been reported that the major virulence f...
Source: Microbiological Research - July 8, 2018 Category: Infectious Diseases Source Type: research

An essential role for hfq involved in biofilm formation and virulence in serotype 4b Listeria monocytogenes
Publication date: Available online 7 July 2018Source: Microbiological ResearchAuthor(s): Hao Yao, Meiqin Kang, Yuting Wang, Youwei Feng, Suwei Kong, Xuexue Cai, Zhiting Ling, Sisi Chen, Xin’an Jiao, Yuelan YinAbstractRegulator factor Hfq has been widely detected among both Gram-positive and Gram-negative bacteria; however, its role in Gram-positive bacteria is less well established and varies among species. In Listeria monocytogenes (Lm), an organism able to adapt to a range of environments and live both saprobiotic and parasitic lifestyles, the role of Hfq is not fully understood. Serotype 4b Listeria monocytogenes ...
Source: Microbiological Research - July 8, 2018 Category: Infectious Diseases Source Type: research

Effectiveness of multi-trait Burkholderia contaminans KNU17BI1 in growth promotion and management of banded leaf and sheath blight in maize seedling
This study for the first time demonstrated potent in vitro antifungal activity of B. contaminans against Rhizoctonia solani AG-1(IA), Pythium graminicola, Fusarium moniliforme, Alternaria alternata, Alternaria solani, Fusarium graminearum, Stemphylium botryosum Wallr, Colletotrichum dematium, Stemphylium lycopersici and Fusarium oxysporum f.sp. melonis. Furthermore, in this study, for the first time, the potential of B. contaminans stain KNU17BI1 in controlling banded leaf and sheath blight of maize caused by R. solani AG-1(IA) was reported. Therefore, further studies are warranted on the structural identification of actua...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Comparison of volatile compounds released by entomopathogenic fungi
Publication date: Available online 24 June 2018Source: Microbiological ResearchAuthor(s): Aleksandra Bojke, Cezary Tkaczuk, Piotr Stepnowski, Marek GołębiowskiA B S T R A C TEntomopathogenic fungi are fungal species which are used as a potential source of biopesticides. These fungi produce secondary metabolites which in insects can cause disruption in the normal functioning of their bodies, disease or even death. In order to fully characterize the physiology of entomopathogenic fungi we should identify the volatile organic compounds which are involved in this process. Therefore, we conducted a qualitative and quantitativ...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

A toxin-antitoxin system is essential for the stability of mosquitocidal plasmid pBsph of Lysinibacillus sphaericus
In this study, we identified that orf9 and orf10 are necessary for the stability of pBsph by a series of deletion and complementation experiments. Bioinformatics analysis showed that ORF9 contains a PIN domain of VapBC toxin-antitoxin (TA) system, whereas ORF10 share no significant sequence similarity to any of the characterized antitoxins in the database. Further studies revealed that orf9 and orf10 are transcribed as an operon. The overexpression of ORF9 repressed the growth of both Escherichia coli and L. sphaericus, which can be alleviated by overexpression of ORF10. The deletion of orf10 individually or orf9-10 togeth...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Clioquinol induces G2/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Chongjia Yan, Song Wang, Jian Wang, Hui Li, Zhiwei Huang, Jing Sun, Min Peng, Wenbin Liu, Ping ShiAbstractClioquinol (CQ) has been used as a classical antimicrobial agent for many years. However, its mode of action is still unclear. In our study, the growth of Candida albicans and Saccharomyces cerevisiae was inhibited by CQ. It did not kill yeast cells, but shortened G1 phase and arrested cell cycle at G2/M phase. By using two-dimensional electrophoresis based proteomic approach, six proteins were found to be significantly affected by C...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max)
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Yuan Hui Liu, En Tao Wang, Yin Shan Jiao, Chang Fu Tian, Lei Wang, Zi Jian Wang, Jia Jing Guan, Raghvendra Pratap Singh, Wen Xin Chen, Wen Feng ChenAbstractSite-specific insertion plasmid pVO155 was used to knockout the genes involved in the alternation of host range of strain Bradyrhizobium diazoefficiens USDA 110 from its original determinate-nodule-forming host soybean (Glycine max), to promiscuous and indeterminate-nodule-forming shrubby legume sophora (Sophora flavescens). Symbiotic phenotypes of these mutants inoculated to these tw...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Renu Verma, Thaís Cabrera Galvão Rojas, Renato Pariz Maluta, Janaína Luisa Leite, Gerson Nakazato, Wanderley Dias de SilveiraAbstractAvian Pathogenic Escherichia coli (APEC) strains belong to the extra-intestinal pathogenic group of E. coli (ExPEC) that causes colibacillosis in poultry. A variety of putative virulence factors of APEC are recognized as potent causes of pathogenicity, the mechanisms underlying their pathogenicity are still not fully understood. The role of yicS in the virulence of pathogenic E. coli is...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Implication of orphan histidine kinase (OhkAsp) in biosynthesis of doxorubicin and daunorubicin in Streptomyces peucetius ATCC 27952
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Anaya Raj Pokhrel, Hue Thi Nguyen, Dipesh Dhakal, Amit Kumar Chaudhary, Jae Kyung SohngAbstractThe orphan histidine kinase (HK) from Streptomyces peucetius ATCC 27952 (ohkAsp) was found to be implicated in the regulation of doxorubicin (DOX)/daunorubicin (DNR) biosynthesis, self-defense and developmental attributes. OhkAsp is a homolog of OhkA from Streptomyces coelicolor and Streptomyces avermitilis (with 73 and 75% identity). As in its homologs, S. peucetius mutant with deletion of ohkAsp was found to enhance metabolite biosynthesis an...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Community structure and plant growth-promoting potential of cultivable bacteria isolated from Cameroon soil
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Gylaine Vanissa Tchuisseu Tchakounté, Beatrice Berger, Sascha Patz, Henri Fankem, Silke RuppelAbstractExploiting native plant growth-promoting rhizobacteria (PGPR) in Cameroonian agro-ecosystems provides a means to improve plant–microbe interactions that may enhance ecosystem sustainability and agricultural productivity in an environmentally eco-friendly way. Consequently, we aimed to investigate the community structure and functional PGPR diversity of maize grown in Cameroon. Native bacteria isolated from Cameroon maize rhi...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

LotS/LotR/Clp, a novel signal pathway responding to temperature, modulating protease expression via c-di-GMP mediated manner in Stenotrophomonas maltophilia FF11
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Qingling Wang, Fangling Ji, Jianli Guo, Yuepeng Wang, Yanyan Li, Jingyun Wang, Lijia An, Yongming BaoAbstractStenotrophomonas maltophilia as one of increasing food spoilage bacteria and fish pathogens has become a threat to aquiculture industry. A major factor contributing to the success of bacterium is its outstanding ability to secrete protease at low temperatures. Here, a cAMP receptor like protein (Clp) shows a positive regulation on this protease, named S. maltophilia temperature-response protease (SmtP). Interestingly, a two-compon...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Brazilian red propolis: Chemical composition and antibacterial activity determined using bioguided fractionation
Publication date: September 2018Source: Microbiological Research, Volume 214Author(s): Luciane Corbellini Rufatto, Paola Luchtenberg, Charlene Garcia, Christine Thomassigny, Sylvie Bouttier, João Antonio Pêgas Henriques, Mariana Roesch-Ely, Françoise Dumas, Sidnei MouraAbstractThe indiscriminate use of antibiotics is causing an increase in bacterial resistance, complicating therapeutic planning. In this context, natural products have emerged as major providers of bioactive compounds. This work performs a bioguided study of Brazilian red propolis to identify compounds with antibacterial potential and to ...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Novel Trichoderma strains isolated from tree barks as potential biocontrol agents and biofertilizers for direct seeded rice
This study is the first time report of utilization of Trichoderma spp. isolated from different tree bark from Odisha state of India for rice crop health management and higher productivity. Six isolates of Trichoderma spp. were identified based on the morphological characteristics and species determination was performed by molecular assays. One of the isolated strains determined as Trichoderma erinaceum outperformed others. Trichoderma erinaceum controlled three soil borne plant pathogens i.e. Rhizoctonia solani, Sclerotium rolfsii and Sclerotium oryzae effectively under controlled condition and R. solani and Helminthospori...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

The pyrophosphohydrolase RppH is involved in the control of RsmA/CsrA expression in Azotobacter vinelandii and Escherichia coli
This study shows that in the soil bacterium Azotobacter vinelandii, inactivation of rppH gene negatively affected the production of bioplastic poly-β-hydroxybutyrate (PHB) by reducing the expression at the translational level of PhbR, the specific transcriptional activator of the phbBAC biosynthetic operon. The effect of RppH on the translation of phbR seemed to be exerted through the translational repressor RsmA, as the inactivation of rsmA in the rppH mutant restored the phbR expression. Interestingly, in Escherichia coli inactivation of rppH also affected the expression of CsrA, the RsmA homolog. The level of the c...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Growth stage and tissue specific colonization of endophytic bacteria having plant growth promoting traits in hybrid and composite maize (Zea mays L.)
In this study endophytic bacteria were isolated from a hybrid PEEHM-5 and composite PC-4 maize varieties using root, stem and leaf tissues of plants at vegetative, flowering and maturity stages of growth. PEEHM-5 harbored higher endophytic bacterial population than PC-4 at all growth stages, with highest in roots and at flowering stage. Morphologically 188 different endophytic isolates (82 from PEEHM-5, 106 from PC-4) were screened for plant growth promoting attributes viz. P, K, Zn solubilization, production of hormones, siderophore, ACC deaminase, HCN, biological nitrogen fixation and biocontrol of two maize fungal patho...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Brevibacterium linens RS16 confers salt tolerance to Oryza sativa genotypes by regulating antioxidant defense and H+ ATPase activity
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Poulami Chatterjee, Sandipan Samaddar, Ülo Niinemets, Tong-Min SaAbstractSoil salinity is one of the major limitations that affects both plant and its soil environment, leading to reduced agricultural production. Evaluation of stress severity by plant physical and biochemical characteristics is an established way to study plant-salt stress interaction, but the halotolerant properties of plant growth promoting bacteria (PGPB) along with plant growth promotion is less studied till date. The aim of the present study was to elucidate the ...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Inhibition of biofilm formation by Cd2+ on Bacillus subtilis 1JN2 depressed its biocontrol efficiency against Ralstonia wilt on tomato
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Wei Yang, Haixia Yan, Ji Zhang, Yuming Gao, Wei Xu, Jinshan Shang, Yuming LuoAbstractBacillus subtilis 1JN2 can serve as an effective biocontrol agent against Ralstonia wilt on tomato, but the efficiency of control depends on the levels of heavy metals in the rhizosphere soil. Here, we investigated how the heavy metal Cd2+ affects the biocontrol efficacy of B.subtilis 1JN2 on Ralstonia wilt. We found that low Cd2+ content of 2 mM or lower had no effects on the biofilm formation of 1JN2, while media containing 3 mM or higher Cd2+ levels...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Current insight and futuristic vistas of microbial transglutaminase in nutraceutical industry
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Syeda Warisul Fatima, Sunil K. KhareAbstractMicrobial transglutaminase (MTGase) has become a driving force in the food industry cross-linking the food proteins. MTGase-the nature’s molecular glue is recognized to reorient food protein’s functional properties without affecting its nutritive value. The scope and approach of this review is to have insight on the action mechanism of MTGase and impact of molecular linkage on functional proteins in various protein moieties in development of innovative features in food production for ...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Hydroxycoumarins: New, effective plant-derived compounds reduce Ralstonia pseudosolanacearum populations and control tobacco bacterial wilt
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Liang Yang, Lintong Wu, Xiaoyuan Yao, Shiyuan Zhao, Jiao Wang, Shili Li, Wei DingAbstractPlant wilt disease caused by the soilborne bacterial pathogen Ralstonia pseudosolanacearum is one of the most devastating plant diseases; however, no effective protection against this disease has been developed. Coumarins are important natural plant-derived compounds with a wide range of bioactivities and extensive applications in medicine and agriculture. In the present study, three hydroxycoumarins (Hycs), umbelliferone (UM), esculetin (ES) and daphn...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Bacillus thuringiensis produces the lipopeptide thumolycin to antagonize microbes and nematodes
In this study, a new lipopeptide biosynthesis gene cluster in B. thuringiensis BMB171 was discovered by genome mining and verified by reverse genetics. Thumolycin, the lipopeptide synthesized by this gene cluster, was then isolated and purified. Mass spectrum analysis revealed the molecular mass of thumolycin is 696.51 Da with the predicted molecular formula of C38H64N8O4. Further bioactivities assay showed that thumolycin endowed B. thuringiensis BMB171 with broad spectrum antimicrobial and nematocidal activities. (Source: Microbiological Research)
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Matricaria genus as a source of antimicrobial agents: From farm to pharmacy and food applications
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Mehdi Sharifi-Rad, Jolanta Nazaruk, Letizia Polito, Maria Flaviana Bezerra Morais-Braga, Janaína Esmeraldo Rocha, Henrique Douglas Melo Coutinho, Bahare Salehi, Giulia Tabanelli, Chiara Montanari, María del Mar Contreras, Zubaida Yousaf, William N. Setzer, Deepa R. Verma, Miquel Martorell, Antoni Sureda, Javad Sharifi-RadAbstractMatricaria is a widespread genus of flowering plants of the family Asteraceae that grow in temperate regions of Europe, Asia, America and Africa. Some of the species are also naturalized in Australia....
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Antibiotic producing endophytic Streptomyces spp. colonize above-ground plant parts and promote shoot growth in multiple healthy and pathogen-challenged cereal crops
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Janki K. Patel, Sheeba Madaan, G. ArchanaAbstractThe Streptomyces spp. used in this work were previously isolated as diazotrophic endophytes from sorghum stems. Here, we characterized the Streptomyces spp. for their colonization ability, plant growth promotion and protection against fungal disease in three cereals. In vitro analysis by dual culture study showed inhibitory effect on the rice pathogen Magnaporthe oryzae B157 along with inhibition of the ubiquitous phytopathogen Rhizoctonia solani by the Streptomyces spp. used in this study. ...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): How Lie Ling, Zaidah Rahmat, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, Rosli Md. IlliasAbstractBacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding of bacterial acclimation to alkaline media through analyzing extracellular proteins of B. lehensis. The pH range for B. lehensis growth was conduc...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Pyrrolnitrin is more essential than phenazines for Pseudomonas chlororaphis G05 in its suppression of Fusarium graminearum
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Run Huang, Zhibin Feng, Xiaoyan Chi, Xiaoqiang Sun, Yang Lu, Baoshen Zhang, Ruiyang Lu, Wangtai Luo, Yanhua Wang, Jing Miao, Yihe GeAbstractFusarium graminearum is the major causal agent of Fusarium head blight (FHB) disease in cereal crops worldwide. Infection with this fungal phytopathogen can regularly cause severe yield and quality losses and mycotoxin contamination in grains. In previous other studies, one research group reported that pyrrolnitrin had an ability to suppress of mycelial growth of F. graminearum. Other groups revealed t...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Designer probiotic Lactobacillus plantarum expressing oxalate decarboxylase developed using group II intron degrades intestinal oxalate in hyperoxaluric rats
In conclusion, the study demonstrates that food-grade L. plantarum secreting OxdC is capable of degrading intestinal oxalate and thereby prevent CaOx stone formation in experimental rats. (Source: Microbiological Research)
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Bacteria and bacterial products: Foe and friends to Caenorhabditis elegans
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Fazlurrahman Khan, Saurabh Jain, Sandra Folarin OloketuyiAbstractCaenorhabditis elegans is a model organism for the study of different molecular, biochemical, microbial and immunity-related mechanisms. In its natural habitat, C. elegans survives by feeding microorganisms (mainly bacteria), though majorly on Escherichia coli OP50 when grown in the laboratory. Numerous bacteria are shown to influence the lifespan, behavioural responses and innate immunity of C. elegans. The secondary metabolites produced by bacteria have shown to play key ro...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

Specific growth inhibitors of Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, and Clavibacter michiganensis subsp. michiganensis
In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at>5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E co...
Source: Microbiological Research - July 5, 2018 Category: Infectious Diseases Source Type: research

A toxin-antitoxin system is essential for the stability of mosquitocidal plasmid pBsph of Lysinibacillus sphaericus
In this study, we identified that orf9 and orf10 are necessary for the stability of pBsph by a series of deletion and complementation experiments. Bioinformatics analysis showed that ORF9 contains a PIN domain of VapBC toxin-antitoxin (TA) system, whereas ORF10 share no significant sequence similarity to any of the characterized antitoxins in the database. Further studies revealed that orf9 and orf10 are transcribed as an operon. The overexpression of ORF9 repressed the growth of both Escherichia coli and L. sphaericus, which can be alleviated by overexpression of ORF10. The deletion of orf10 individually or orf9-10 togeth...
Source: Microbiological Research - June 28, 2018 Category: Infectious Diseases Source Type: research

Matricariagenus as a source of antimicrobial agents: From farm to pharmacy and food applications
Publication date: October 2018 Source:Microbiological Research, Volume 215 Author(s): Mehdi Sharifi-Rad, Jolanta Nazaruk, Letizia Polito, Maria Flaviana Bezerra Morais-Braga, Janaína Esmeraldo Rocha, Henrique Douglas Melo Coutinho, Bahare Salehi, Giulia Tabanelli, Chiara Montanari, María del Mar Contreras, Zubaida Yousaf, William N. Setzer, Deepa R. Verma, Miquel Martorell, Antoni Sureda, Javad Sharifi-Rad Matricaria is a widespread genus of flowering plants of the family Asteraceae that grow in temperate regions of Europe, Asia, America and Africa. Some of the species are also naturalized in Australia. Some...
Source: Microbiological Research - June 28, 2018 Category: Infectious Diseases Source Type: research

Bacteria and bacterial products: Foe and friends to Caenorhabditis elegans
Publication date: Available online 25 June 2018 Source:Microbiological Research Author(s): Fazlurrahman Khan, Saurabh Jain, Sandra Folarin Oloketuyi Caenorhabditis elegans is a model organism for the study of different molecular, biochemical, microbial and immunity-related mechanisms. In its natural habitat, C. elegans survives by feeding microorganisms (mainly bacteria), though majorly on Escherichia coli OP50 when grown in the laboratory. Numerous bacteria are shown to influence the lifespan, behavioural responses and innate immunity of C. elegans. The secondary metabolites produced by bacteria have shown to play key ro...
Source: Microbiological Research - June 25, 2018 Category: Infectious Diseases Source Type: research

Matricaria genus as a source of antimicrobial agents: From Farm to Pharmacy and Food Applications
Publication date: Available online 25 June 2018 Source:Microbiological Research Author(s): Mehdi Sharifi-Rad, Jolanta Nazaruk, Letizia Polito, Maria Flaviana Bezerra Morais-Braga, Janaína Esmeraldo Rocha, Henrique Douglas Melo Coutinho, Bahare Salehi, Giulia Tabanelli, Chiara Montanari, María del Mar Contreras, Zubaida Yousaf, William N. Setzer, Deepa R. Verma, Miquel Martorell, Antoni Sureda, Javad Sharifi-Rad Matricaria is a widespread genus of flowering plants of the family Asteraceae that grow in temperate regions of Europe, Asia, America and Africa. Some of the species are also naturalized in Australia....
Source: Microbiological Research - June 25, 2018 Category: Infectious Diseases Source Type: research

Comparison of volatile compounds released by entomopathogenic fungi
Publication date: Available online 24 June 2018 Source:Microbiological Research Author(s): Aleksandra Bojke, Cezary Tkaczuk, Piotr Stepnowski, Marek Gołębiowski Entomopathogenic fungi are fungal species which are used as a potential source of biopesticides. These fungi produce secondary metabolites which in insects can cause disruption in the normal functioning of their bodies, disease or even death. In order to fully characterize the physiology of entomopathogenic fungi we should identify the volatile organic compounds which are involved in this process. Therefore, we conducted a qualitative and quantitative analysis o...
Source: Microbiological Research - June 24, 2018 Category: Infectious Diseases Source Type: research

Designer probiotic Lactobacillus plantarum expressing oxalate decarboxylase developed using group II intron degrades intestinal oxalate in hyperoxaluric rats
In conclusion, the study demonstrates that food-grade L. plantarum secreting OxdC is capable of degrading intestinal oxalate and thereby prevent CaOx stone formation in experimental rats. (Source: Microbiological Research)
Source: Microbiological Research - June 22, 2018 Category: Infectious Diseases Source Type: research

Brevibacterium linens RS16 confers salt tolerance to Oryza sativa genotypes by regulating antioxidant defense and H+ ATPase activity
Publication date: Available online 19 June 2018 Source:Microbiological Research Author(s): Poulami Chatterjee, Sandipan Samaddar, Ülo Niinemets, Tong-Min Sa Soil salinity is one of the major limitations that affects both plant and its soil environment, leading to reduced agricultural production. Evaluation of stress severity by plant physical and biochemical characteristics is an established way to study plant-salt stress interaction, but the halotolerant properties of plant growth promoting bacteria (PGPB) along with plant growth promotion is less studied till date. The aim of the present study was to elucidate the ...
Source: Microbiological Research - June 20, 2018 Category: Infectious Diseases Source Type: research

Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes
Publication date: October 2018 Source:Microbiological Research, Volume 215 Author(s): How Lie Ling, Zaidah Rahmat, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, Rosli Md. Illias Bacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding of bacterial acclimation to alkaline media through analyzing extracellular proteins of B. lehensis. The pH range for B. lehensis growth was conducted, ...
Source: Microbiological Research - June 20, 2018 Category: Infectious Diseases Source Type: research

Pyrrolnitrin is more essential than phenazines for Pseudomonas chlororaphis G05 in its suppression of Fusarium graminearum
Publication date: Available online 18 June 2018 Source:Microbiological Research Author(s): Run Huang, Zhibin Feng, Xiaoyan Chi, Xiaoqiang Sun, Yang Lu, Baoshen Zhang, Ruiyang Lu, Wangtai Luo, Yanhua Wang, Jing Miao, Yihe Ge Fusarium graminearum is the major causal agent of Fusarium head blight (FHB) disease in cereal crops worldwide. Infection with this fungal phytopathogen can regularly cause severe yield and quality losses and mycotoxin contamination in grains. In previous other studies, one research group reported that pyrrolnitrin had an ability to suppress of mycelial growth of F. graminearum. Other groups revealed t...
Source: Microbiological Research - June 19, 2018 Category: Infectious Diseases Source Type: research

Specific growth inhibitors of Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, and Clavibacter michiganensis subsp. michiganensis
In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 µg/ml final concentration. The most effective compounds, ralhibitins A, C, ...
Source: Microbiological Research - June 10, 2018 Category: Infectious Diseases Source Type: research

Novel Trichoderma strains isolated from tree barks as potential biocontrol agents and biofertilizers for direct seeded rice
This study is the first time report of utilization of Trichoderma spp. isolated from different tree bark from Odisha state of India for rice crop health management and higher productivity. Six isolates of Trichoderma spp. were identified based on the morphological characteristics and species determination was performed by molecular assays. One of the isolated strains determined as Trichoderma erinaceum outperformed others. Trichoderma erinaceum controlled three soil borne plant pathogens i.e. Rhizoctonia solani, Sclerotium rolfsii and Sclerotium oryzae effectively under controlled condition and R. solani and Helminthospori...
Source: Microbiological Research - June 6, 2018 Category: Infectious Diseases Source Type: research

Current Insight and Futuristic Vistas of Microbial Transglutaminase in Nutraceutical Industry
Publication date: Available online 4 June 2018 Source:Microbiological Research Author(s): Syeda Warisul Fatima, Sunil K. Khare Microbial transglutaminase (MTGase) has become a driving force in the food industry cross-linking the food proteins. MTGase-the nature’s molecular glue is recognized to reorient food protein’s functional properties without affecting its nutritive value. The scope and approach of this review is to have insight on the action mechanism of MTGase and impact of molecular linkage on functional proteins in various protein moieties in development of innovative features in food production for b...
Source: Microbiological Research - June 4, 2018 Category: Infectious Diseases Source Type: research

Bacillus thuringiensis produces the lipopeptide thumolycin to antagonize microbes and nematodes
In this study, a new lipopeptide biosynthesis gene cluster in B. thuringiensis BMB171 was discovered by genome mining and verified by reverse genetics. Thumolycin, the lipopeptide synthesized by this gene cluster, was then isolated and purified. Mass spectrum analysis revealed the molecular mass of thumolycin is 696.51 Da with the predicted molecular formula of C38H64N8O4. Further bioactivities assay showed that thumolycin endowed B. thuringiensis BMB171 with broad spectrum antimicrobial and nematocidal activities. (Source: Microbiological Research)
Source: Microbiological Research - June 3, 2018 Category: Infectious Diseases Source Type: research

Antibiotic producing endophytic Streptomyces spp. colonize above-ground plant parts and promote shoot growth in multiple healthy and pathogen-challenged cereal crops
Publication date: Available online 3 June 2018 Source:Microbiological Research Author(s): Janki K. Patel, Sheeba Madaan, G. Archana The Streptomyces spp. used in this work were previously isolated as diazotrophic endophytes from sorghum stems. Here, we characterize the Streptomyces spp. for their colonization ability, plant growth promotion and protection against fungal disease in three cereals. In vitro analysis by dual culture study showed inhibitory effect on the rice pathogen Magnaporthe oryzae B157 and along with inhibition of the ubiquitous phytopathogen Rhizoctonia solani by the Streptomyces spp. used in this study...
Source: Microbiological Research - June 3, 2018 Category: Infectious Diseases Source Type: research