Characterization the role of GacA-dependent small RNAs and RsmA family proteins on 2,4-diacetylphloroglucinol production in Pseudomonas fluorescens 2P24
Publication date: Available online 9 December 2019Source: Microbiological ResearchAuthor(s): Yang Zhang, Bo Zhang, Xiaogang Wu, Li-Qun ZhangAbstractPseudomonas fluorescens 2P24 is a plant-beneficial rhizobacteria that controls many root diseases caused by soil-borne pathogens, and the production of the antibiotic compound 2,4-diacetylphloroglucinol (2,4-DAPG) is essential for its biocontrol ability. In the present study, we investigated the regulatory mechanism acting on the production of 2,4-DAPG by the GacA-dependent small non-coding RNAs (sRNAs) and RsmA/E proteins in strain 2P24. Our results showed that the GacS-GacA s...
Source: Microbiological Research - December 9, 2019 Category: Infectious Diseases Source Type: research

The cell surface adhesins of Mycobacterium tuberculosis
Publication date: Available online 9 December 2019Source: Microbiological ResearchAuthor(s): Vivek Vinod, Sukhithasri Vijayrajratnam, Anil Kumar Vasudevan, Raja BiswasAbstractBacterial cell surface adhesins play a major role in facilitating host colonization and subsequent establishment of infection. The surface of Mycobacterium tuberculosis, owing to the complex architecture of its cell envelope, expresses numerous adhesins with varied chemical nature, including proteins, lipids, lipoproteins, glycoproteins and glycopolymers. Studies on mycobacterial adhesins show that they bind with multifarious host receptors and extrac...
Source: Microbiological Research - December 9, 2019 Category: Infectious Diseases Source Type: research

Community structures of the rhizomicrobiomes of cultivated and wild soybeans in their continuous cropping
In this study, we simulated monocropping of cultivated and wild soybeans under greenhouse conditions to investigate the rhizomicrobiomes of both soybeans. Results indicated that the bacterial community structure still maintained a changing trend after four continuous planting seasons, while fungal community structure showed a stable trend as indicated by the high similarity in the fungal community structure between the third and fourth planting rotations in both soybeans. In addition, by comparing the continuous cropping of the two soybeans, we found different fungal groups in their rhizospheres between the wild and cultiv...
Source: Microbiological Research - December 8, 2019 Category: Infectious Diseases Source Type: research

Functional Characterization of Potential PGPR Exhibiting Broad-spectrum Antifungal activity
This study describes the biocontrol potential ofrhizobacteria against a range of fungal phytopathogens. Out of 227 bacteria isolated from the rhizosphere of maize, rice, wheat, potato, sunflower and soybean crops cultivated in different agro-ecological regions of Pakistan, 48 exhibited>60% antifungal activity againstFusarium oxysporum, Fusarium moniliforme, Rhizoctonia solani, Colletotrichum gloeosporioides, Colletotrichum falcatum, Aspergillus niger, and Aspergillus flavus. The rhizobacteria inhibiting> 65% pathogen growth were selected for detailed molecular and in planta studies most of which were identified as Ps...
Source: Microbiological Research - December 2, 2019 Category: Infectious Diseases Source Type: research

Rhizobacteria-induced systemic tolerance against drought stress in Sorghum bicolor (L.) Moench
Publication date: Available online 30 November 2019Source: Microbiological ResearchAuthor(s): René Carlson, Fidele Tugizimana, Paul A. Steenkamp, Ian A. Dubery, Ahmed Idris Hassen, Nico LabuschagneAbstractInduction of systemic tolerance in sorghum [Sorghum bicolor (L.) Moench] against drought stress was studied by screening a large collection of rhizobacterial isolates for their potential to exhibit this essential plant growth-promoting trait. This was done by means of a greenhouse assay that measured the relative change in both plant height and -biomass (roots and shoots) between rhizobacteria-primed versus non-pri...
Source: Microbiological Research - December 2, 2019 Category: Infectious Diseases Source Type: research

Inhibition of biofilm- and hyphal- development, two virulent features of Candida albicans by secondary metabolites of an endophytic fungus Alternaria tenuissima having broad spectrum antifungal potential
Publication date: Available online 28 November 2019Source: Microbiological ResearchAuthor(s): Sohini Chatterjee, Ranjan Ghosh, Narayan Chandra MandalAbstractFungal resistance against frequently used antifungal medicines used for invasive candidiasis and other fungal infections is directing scientist for searching and developing novel antifungal drugs. An endophytic fungal strain Alternaria tenuissima OE7 has been isolated from leaves of Ocimum tenuiflorum L. which showed antifungal activity against numbers of human pathogenic fungi including Trichophyton rubrum, Microsporum gypseum, Aspergillus parasiticus, A. flavus, A. f...
Source: Microbiological Research - November 30, 2019 Category: Infectious Diseases Source Type: research

Ecological impact of a rare sugar on grapevine phyllosphere microbial communities
Publication date: Available online 26 November 2019Source: Microbiological ResearchAuthor(s): Michele Perazzolli, Andrea Nesler, Oscar Giovannini, Livio Antonielli, Gerardo Puopolo, Ilaria PertotAbstractPlants host a complex microbiota inside or outside their tissues, and phyllosphere microorganisms can be influenced by environmental, nutritional and agronomic factors. Rare sugars are defined as monosaccharides with limited availability in nature and they are metabolised by only few certain microbial taxa. Among rare sugars, tagatose (TAG) is a low-calories sweetener that stimulates and inhibits beneficial and pathogenic b...
Source: Microbiological Research - November 26, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: January 2020Source: Microbiological Research, Volume 230Author(s): (Source: Microbiological Research)
Source: Microbiological Research - November 20, 2019 Category: Infectious Diseases Source Type: research

Identification of mimp-associated effector genes in Fusarium oxysporum f. sp. cubense race 1 and race 4 and virulence confirmation of a candidate effector gene
In this study, genome-wide effector gene identification was performed in Foc race 1 (Foc 1) and Foc race 4 (Foc 4) based on the context association between the effector genes and the transposable element mimp. A total of 20 candidate effector genes were identified, of which 3 were Foc 1-specific, 6 were Foc 4-specific, and 11 were present in both Foc 1 and Foc 4. Most genes (14 out of 20) showed a significant transcriptional burst in planta compared with in-culture conditions, from more than 10-fold to 1,617-fold, and at the highest 32,725-fold. In addition to Foc 1- and Foc 4-specific genes, the genes Foc 283, Foc 495, an...
Source: Microbiological Research - November 19, 2019 Category: Infectious Diseases Source Type: research

Molecular and Biotechnological Aspects of Secondary Metabolites in Actinobacteria
Publication date: Available online 12 November 2019Source: Microbiological ResearchAuthor(s): Richa Salwan, Vivek SharmaAbstractThe ability to produce plethora of secondary metabolites and enzymes for pharmaceutical, agricultural and biotechnological applications make actinobacteria one of the most explored microbes among prokaryotes. The secondary metabolites and lytic enzymes of actinobacteria are known for their role in various physiological, cellular and biological processes including environmental sensing, mineral acquisition and recycling, and establishing social communication. In addition, the basic scaffold of seco...
Source: Microbiological Research - November 14, 2019 Category: Infectious Diseases Source Type: research

Biochar amendment controlled bacterial wilt through changing soil chemical properties and microbial community
This study revealed the potential of biochar in control of bacterial wilt. (Source: Microbiological Research)
Source: Microbiological Research - November 13, 2019 Category: Infectious Diseases Source Type: research

Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656
In this study, we focused on mating-type switching and gene expression in order to elucidate its sexual life cycle and establish genetic approaches applicable for the strain. A putative mating-type gene cluster was identified in TBRC656 that is syntenic to the cluster in Ogataea parapolymorpha DL-1 (previously named Hansenula polymorpha). Like DL-1, TBRC656 possesses two mating loci, namely MATa and MATα, and also shows flip-flop mating-type switching. Interestingly, unlike any other methylotrophic yeast, TBRC656 robustly switched mating type during late growth in rich medium (YPD). Under nutrient depletion, mating-t...
Source: Microbiological Research - November 11, 2019 Category: Infectious Diseases Source Type: research

Colonization of the Gut Microbiota of Honey Bee (Apis mellifera) Workers at Different Developmental Stages
In this study, we explored the dynamic changes of the gut microbiota of A. mellifera at different developmental stages covering the whole life cycle using high-throughput 16S rRNA gene sequencing. The results indicated that the core (shared) gut microbiota changes significantly among different developmental stages. The diversity of the bacterial community in workers among different ages was significantly different. In addition, by comparing the core gut microbiota among different-aged workers, we found that newly emerged workers had fewer core microbiota. Three genera, Gilliamella, Frischella, and Snodgrassella, were signi...
Source: Microbiological Research - November 7, 2019 Category: Infectious Diseases Source Type: research

Isolation of Trichoderma from forestry model base and the antifungal properties of isolate TpsT17 toward Fusarium oxysporum
Publication date: Available online 5 November 2019Source: Microbiological ResearchAuthor(s): Chang Zhou, Ruiting Guo, Shida Ji, Haijuan Fan, Jinjie Wang, Yucheng Wang, Zhihua LiuAbstractEleven soil samples were collected from different plantations at the Forestry Model Base, Northeast Forestry University, China (45°43ʹ10″ N, 126°37ʹ15″ E), and 122 Trichoderma strains (T1–T122) were isolated. Nine Trichoderma species were identified based on morphological and molecular classification methods. The diversity of woody fungi was analyzed based on the type and quantity of Trichoderma spp. in the soil ...
Source: Microbiological Research - November 7, 2019 Category: Infectious Diseases Source Type: research

The potential of endophytic fungi isolated from cucurbit plants for biocontrol of soilborne fungal diseases of cucumber
In this study, 82 endophytic fungal strains had good disease suppressive effects and no obvious adverse effects on cucumber growth, and 35 of them showed obvious growth-promoting effects, which suggested that endophytic fungi from cucurbit plants have excellent potential for plant disease control. (Source: Microbiological Research)
Source: Microbiological Research - November 5, 2019 Category: Infectious Diseases Source Type: research

Illumina sequencing of 16S rRNA tag shows disparity in rhizobial and non-rhizobial diversity associated with root nodules of mung bean (Vigna radiata L.) growing in different habitats in Pakistan
Publication date: Available online 23 October 2019Source: Microbiological ResearchAuthor(s): Sughra Hakim, Babur S. Mirza, Asma Imran, Ahmad Zaheer, Sumera Yasmin, Fathia Mubeen, Joan E. Mclean, M. Sajjad MirzaAbstractIn Rhizobium-legume symbiosis, the nodule is the most frequently studied compartment, where the endophytic/symbiotic microbiota demands critical investigation for development of specific inocula. We identified the bacterial diversity within root nodules of mung bean from different growing areas of Pakistan using Illumina sequencing of 16S rRNA gene. We observed specific OTUs related to specific site where Bra...
Source: Microbiological Research - October 24, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: December 2019Source: Microbiological Research, Volume 229Author(s): (Source: Microbiological Research)
Source: Microbiological Research - October 23, 2019 Category: Infectious Diseases Source Type: research

CpALS4770 and CpALS4780 contribution to the virulence of Candida parapsilosis
Publication date: Available online 18 October 2019Source: Microbiological ResearchAuthor(s): Marina Zoppo, Mariagrazia Di Luca, Mauro Franco, Cosmeri Rizzato, Antonella Lupetti, Annarita Stringaro, Flavia De Bernardis, Christoph Schaudinn, M. Inmaculada Barrasa, Daria Bottai, Valmik K. Vyas, Arianna TavantiAbstractThe ability of yeast to adhere to biotic and abiotic surfaces represents an essential trait during the early stages of infection. Agglutinin-like sequence (Als) cell-wall proteins play a key role in adhesion of Candida species. Candida parapsilosis genome encompasses 5 ALS members, of which only the role of CPAR2...
Source: Microbiological Research - October 20, 2019 Category: Infectious Diseases Source Type: research

Arbuscular mycorrhizal fungi (Glomus intraradices) and diazotrophic bacterium (Rhizobium BMBS) primed defense in blackgram against herbivorous insect (Spodoptera litura) infestation
Publication date: Available online 17 October 2019Source: Microbiological ResearchAuthor(s): Selvaraj Anandakumar, Thangavel Kalaiselvi, Uthandi SivakumarAbstractIn the changing scenario of agriculture, plants are exposed to various biotic and abiotic stresses. Induction of both constitutive and inducible defense systems was noticed in plants exposed to stress. As a major defense response, production of phenolics and superoxide radicals quenching enzymes is accelerated in plants under stress. These metabolites production intensified further when arbuscular mycorrhizal fungi (AMF) infected plants are subjected to stress. Wi...
Source: Microbiological Research - October 18, 2019 Category: Infectious Diseases Source Type: research

Molecular and morphological characterization of rice phylloplane fungi and determination of the antagonistic activity against rice pathogens
Publication date: Available online 15 October 2019Source: Microbiological ResearchAuthor(s): Amanda A. Chaibub, Thatyane P. de Sousa, Leila G. de Araújo, Marta Cristina C. de FilippiAbstractCladosporium spp. is a cosmopolitan fungal genus. In the literature, it has been reported as a biological agent for controlling several plant diseases, but its mechanism of action has never been clarified. The present study aims to identify Cladosporium spp. based on the DNA phylogeny of nine isolates obtained from the phylloplane of rice and their potential antagonistic activity against the main fungal pathogens that affect rice...
Source: Microbiological Research - October 17, 2019 Category: Infectious Diseases Source Type: research

Elucidation of quorum sensing components and their role in regulation of symbiotically important traits in Ensifer nodulating pigeon pea
This study highlights that Ensifer nodulating pigeon pea, although with significant variance in the anatomy of their QS components, regulate symbiotically crucial cell-processes via QS in a scheme that is conserved in multiple genera. (Source: Microbiological Research)
Source: Microbiological Research - October 15, 2019 Category: Infectious Diseases Source Type: research

Deactivation and mislocalization of Toxoplasma gondii rhoptry protein 18 induced by a single amino acid mutation on the proton transport catalytic aspartic acid
Publication date: Available online 13 October 2019Source: Microbiological ResearchAuthor(s): Yang Zhang, Bo Shiun Lai, Mario JuhasAbstractRhoptry protein 18 (ROP18) is a major determinant of strain-specific virulence in Toxoplasma gondii. The kinase activity of ROP18 is required for acute virulence, while the aspartate in the catalytic loop of ROP18 is considered essential for phosphoryl transfer. We showed that a single amino acid mutation at the catalytic aspartate residue (D409A mutation) significantly altered ROP18 kinase activity in vitro, and abolished ROP18-mediated ATF6β degradation. Furthermore, the investiga...
Source: Microbiological Research - October 14, 2019 Category: Infectious Diseases Source Type: research

Outer membrane protein OmpU is related to iron balance in Vibrio alginolyticus
Publication date: Available online 11 October 2019Source: Microbiological ResearchAuthor(s): Tengteng Lv, Fa Dai, Qiuting Zhuang, Xuelin Zhao, Yina Shao, Ming Guo, Zhimeng Lv, Chenghua Li, Weiwei ZhangAbstractOuter membrane protein U (OmpU) is a major porin from Vibrio alginolyticus and has been considered a vaccine candidate against infection by V. alginolyticus. After pre-incubated with polyclonal antibody against rOmpU, V. alginolyticus showed a 78% decrease in extracellular iron level, suggesting that interruption of OmpU could increase intracellular iron level. The mRNA expression of ompU under iron-limited conditions...
Source: Microbiological Research - October 12, 2019 Category: Infectious Diseases Source Type: research

PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida
In this study, a pull-down assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida. (Source: ...
Source: Microbiological Research - October 11, 2019 Category: Infectious Diseases Source Type: research

Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum
In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains. In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25–30%) than those of the WT and CK strains. Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only ...
Source: Microbiological Research - October 3, 2019 Category: Infectious Diseases Source Type: research

Inhibition of Rhizoctonia solani RhCh-14 and Pythium ultimum PyFr-14 by Paenibacillus polymyxa NMA1017 and Burkholderia cenocepacia CACua-24: A proposal for biocontrol of phytopathogenic fungi
Publication date: Available online 26 September 2019Source: Microbiological ResearchAuthor(s): Belén Chávez-Ramírez, Jeniffer Chirs Kerber-Díaz, Marí Carmen Acoltzi-Conde, J. Antonio Ibarra, María-Soledad Vásquez-Murrieta, Paulina Estrada-de los SantosAbstractBiocontrol has emerged in recent years as an alternative to pesticides. Given the importance of environmental preservation using biocontrol, in this study two antagonistic bacteria against phytopathogenic fungi were isolated and evaluated. These bacterial strains, identified as Paenibacillus polymyxa NMA1017 and Burkhol...
Source: Microbiological Research - September 28, 2019 Category: Infectious Diseases Source Type: research

Two pathways for thiosulfate oxidation in the alphaproteobacterial chemolithotroph Paracoccus thiocyanatus SST
Publication date: Available online 23 September 2019Source: Microbiological ResearchAuthor(s): Moidu Jameela Rameez, Prosenjit Pyne, Subhrangshu Mandal, Sumit Chatterjee, Masrure Alam, Sabyasachi Bhattacharya, Nibendu Mondal, Jagannath Sarkar, Wriddhiman GhoshAbstractChemolithotrophic bacteria oxidize various sulfur species for energy and electrons, thereby operationalizing biogeochemical sulfur cycles in nature. The best-studied pathway of bacterial sulfur-chemolithotrophy involves direct oxidation of thiosulfate (S2O32-) to sulfate (SO42-) without any free intermediate. This pathway mediated by SoxXAYZBCD is apparently t...
Source: Microbiological Research - September 25, 2019 Category: Infectious Diseases Source Type: research

Altered Metabolomic Profile of Dual-Species Biofilm: Interactions Between Proteus mirabilis and Candida albicans
In this study, we aimed to determine the interspecies interactions between Proteus mirabilis and Candida albicans. Mono and dual-species biofilms were grown in a microtiter plate and metabolomic analysis of the biofilms was performed. The effects of togetherness of two species on the expression levels of candidal virulence genes and urease and swarming activities of P.mirabilis were investigated. The growth of C.albicans was inhibited by P.mirabilis whereas the growth and swarming activity of P.mirabilis were increased by C.albicans. The inhibition of Candida cell growth was found to be biofilm specific. The alteration was...
Source: Microbiological Research - September 25, 2019 Category: Infectious Diseases Source Type: research

The "fighting Wisdom and bravery" of tailed phage and host in the process of adsorption
Publication date: Available online 19 September 2019Source: Microbiological ResearchAuthor(s): Haojie Ge, Maozhi Hu, Ge Zhao, Yi Du, Nannan Xu, Xiang Chen, Xin’an JiaoAbstractIn the process of bacteriophage and bacteria struggle, adsorption is the key factor to determine who is the winner. In this paper, the molecular mechanism of tailed bacteriophage recognition and adsorption to host and the strategy of “fighting wisdom and courage” between them are reviewed. (Source: Microbiological Research)
Source: Microbiological Research - September 20, 2019 Category: Infectious Diseases Source Type: research

Identification of genes regulated by the two-component system response regulator NarP of Actinobacillus pleuropneumoniae via DNA-affinity-purified sequencing
Publication date: Available online 10 September 2019Source: Microbiological ResearchAuthor(s): Qiuhong Zhang, Qi Huang, Qiong Fang, Haotian Li, Hao Tang, Geng Zou, Dong Wang, Siqi Li, Weicheng Bei, Huanchun Chen, Lu Li, Rui ZhouAbstractIdentifying the direct target genes of response regulators (RRs) of a bacterial two-component system (TCS) is critical to understand the roles of TCS in bacterial environmental adaption and pathogenesis. Actinobacillus pleuropneumoniae is an important respiratory bacterial pathogen that causes considerable economic losses to swine industry worldwide. The targets of A. pleuropneumoniae NarP (...
Source: Microbiological Research - September 11, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: November 2019Source: Microbiological Research, Volume 228Author(s): (Source: Microbiological Research)
Source: Microbiological Research - September 11, 2019 Category: Infectious Diseases Source Type: research

A script for initiating molecular biology studies with non-conventional yeasts based on Saccharomycopsis schoenii
Publication date: Available online 9 September 2019Source: Microbiological ResearchAuthor(s): Yeseren Kayacan, Adam Griffiths, Jürgen WendlandAbstractNon-conventional yeasts (NCYs), i.e. all yeasts other than Saccharomyces cerevisiae, are emerging as novel production strains and gain more and more attention to exploit their unique properties. Yet, these yeasts can hardly compete against the advanced methodology and genetic tool kit available for exploiting and engineering S. cerevisiae. Currently, for many NCYs one has to start from scratch to initiate molecular genetic manipulations, which is often time consuming and...
Source: Microbiological Research - September 11, 2019 Category: Infectious Diseases Source Type: research

Abiotic stress resistance, plant growth promotion and antifungal potential of halotolerant bacteria from a Tunisian solar saltern
In this study, 15 halotolerant bacterial strains endowed with important antimicrobial activities were isolated from Sfax solar saltern (Tunisia). All of these strains were characterized by biochemical and molecular tools aiming to investigate their in-vitro and in-vivo antifungal potentialities, plant growth promotion capabilities and metal tolerance abilities under saline stress condition. The 16S rRNA gene sequencing showed that the isolated strains were affiliated to different phylum and three species were described for the first time as plant growth promoting strains (Idiomarina zobelli FMH6v, Nesterenkonia halotoleran...
Source: Microbiological Research - September 9, 2019 Category: Infectious Diseases Source Type: research

Evaluating Glucose and Mannose Profiles in Candida Species Using Quantum Dots Conjugated with Cramoll Lectin as Fluorescent Nanoprobes
Publication date: Available online 8 September 2019Source: Microbiological ResearchAuthor(s): Weslley F. Oliveira, Mariana P. Cabrera, Natália R.M. Santos, Thiago H. Napoleão, Patrícia M.G. Paiva, Rejane P. Neves, Márcia V. Silva, Beate S. Santos, Luana C.B.B. Coelho, Paulo E. Cabral Filho, Adriana Fontes, Maria T.S. CorreiaAbstractGlycoconjugates found on cell walls of Candida species are fundamental for their pathogenicity. Laborious techniques have been employed to investigate the sugar composition of these microorganisms. Herein, we prepared a nanotool, based on the fluorescence of quantum d...
Source: Microbiological Research - September 9, 2019 Category: Infectious Diseases Source Type: research

Broad-spectrum antimicrobial activity of secondary metabolites produced by Serratia marcescens strains
Publication date: Available online 4 September 2019Source: Microbiological ResearchAuthor(s): Tanya Clements, Thando Ndlovu, Wesaal KhanAbstractThe genus Serratia is a predominantly unexplored source of antimicrobial secondary metabolites. The aim of the current study was thus to isolate and evaluate the antimicrobial properties of biosurfactants produced by Serratia species. Forty-nine (n = 34 pigmented; n = 15 non-pigmented) biosurfactant producing Serratia strains were isolated from environmental sources and selected isolates (n = 11 pigmented; n = 11 non-pigmented...
Source: Microbiological Research - September 5, 2019 Category: Infectious Diseases Source Type: research

EPS bound flavins driven mediated electron transfer in thermophilic Geobacillus sp.
Publication date: December 2019Source: Microbiological Research, Volume 229Author(s): Dummi Mahadevan Gurumurthy, Ram Naresh Bharagava, Ashok Kumar, Bhaskar Singh, Muhammad Ashfaq, Ganesh Dattatraya Saratale, Sikandar I. MullaAbstractThrough extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in-situ change in electrochemistry and ...
Source: Microbiological Research - September 3, 2019 Category: Infectious Diseases Source Type: research

Estimating CO2 and VOCs production of Colletotrichum fragariae and Rhizopus stolonifer grown in cold stored strawberry fruit
In conclusion, CO2 and VOCs released by C. fragariae and R. stolonifer on strawberries could infer the presence of anthracnose and soft rot during storage of the fruit at low temperature. (Source: Microbiological Research)
Source: Microbiological Research - September 1, 2019 Category: Infectious Diseases Source Type: research

Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities
Publication date: Available online 31 August 2019Source: Microbiological ResearchAuthor(s): Weifang Xu, Fei Wang, Meng Zhang, Ting Ou, Ruolin Wang, Gary Strobel, Zhonghuai Xiang, Zeyang Zhou, Jie XieAbstractEndophytic bacteria-based biocontrol is regarded as a potential plant disease management strategy. Present study analyzed the diversity of mulberry endophytic bacteria basing on a culture-dependent approach and further evaluated their antimicrobial and plant growth-promoting (PGP) activities. A total of 608 cultivable endophytic bacteria, belonging to 4 phyla and 36 genera, were isolated from four mulberry cultivars hav...
Source: Microbiological Research - September 1, 2019 Category: Infectious Diseases Source Type: research

Edwardsiella induces microtubule-severing in host epithelial cells
Publication date: Available online 26 August 2019Source: Microbiological ResearchAuthor(s): Priyanka Aggarwal, Lifan Wei, Yuping Cao, Qin Liu, Julian A. Guttman, Qiyao Wang, Ka Yin LeungAbstractEdwardsiella bacteria cause economic losses to a variety of commercially important fish globally. Human infections are rare and result in a gastroenteritis-like illness. Because these bacteria are evolutionarily related to other Enterobacteriaceae and the host cytoskeleton is a common target of enterics, we hypothesized that Edwardsiella may cause similar phenotypes. Here we use HeLa and Caco-2 infection models to show that microtub...
Source: Microbiological Research - August 27, 2019 Category: Infectious Diseases Source Type: research

Morphological and molecular aspects of sclerotial development in the phytopathogenic fungus Sclerotinia sclerotiorum
Publication date: Available online 26 August 2019Source: Microbiological ResearchAuthor(s): Bruna Sousa Melo, Aline Raquel Voltan, Walquiria Arruda, Fabyano Alvares Cardoso Lopes, Raphaela Georg, Cirano José UlhoaABSTRACTSclerotinia sclerotiorum (Lib.) de Bary produces a resistance structure called sclerotium, which guarantees its survival in soil for long periods. Morphological and melanization aspects during sclerotial development were evaluated by microscopy and qRT-PCR techniques. S. sclerotiorum produces sclerotia with different phases of maturation and melanization during growth in PDA medium. Using scanning e...
Source: Microbiological Research - August 27, 2019 Category: Infectious Diseases Source Type: research

Regulation of metE+ mRNA Expression by FnrS small RNA in Salmonella enterica serovar Typhimurium
Publication date: Available online 24 August 2019Source: Microbiological ResearchAuthor(s): Chia-Wei Chien, Yu-Feng Chan, Po-Shu Shih, Jung-En Kuan, Ke-Feng Wu, Cindy Wu, Whei-Fen WuAbstractMethionine is critical for variety of metabolic processes in biological organisms, acting as a precursor or intermediate for many final products. The last step for the synthesis of methionine is the methylation of homocysteine, which is catalyzed by MetE. Here, we use Salmonella enterica serovar Typhimurium LT2 to study the regulation of the metE+ gene by an anaerobically induced small non-coding RNA-FnrS, the expression of which is str...
Source: Microbiological Research - August 26, 2019 Category: Infectious Diseases Source Type: research

EPS bound flavins driven mediated electron transfer in thermophilic Geobacillus sp
Publication date: Available online 24 August 2019Source: Microbiological ResearchAuthor(s): Dummi Mahadevan Gurumurthy, Ram Naresh Bharagava, Ashok Kumar, Bhaskar Singh, Muhammad Ashfaq, Ganesh Dattatraya Saratale, Sikandar I. MullaAbstractThrough extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in situ change in electrochemistr...
Source: Microbiological Research - August 26, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: October 2019Source: Microbiological Research, Volume 227Author(s): (Source: Microbiological Research)
Source: Microbiological Research - August 15, 2019 Category: Infectious Diseases Source Type: research

Exploitation of potential bioactive compounds from two soil derived actinomycetes, Streptomyces sp. strain 196 and RI.24
Publication date: Available online 12 August 2019Source: Microbiological ResearchAuthor(s): Prateek Kumar, Aditi Kundu, Munendra Kumar, Renu Solanki, Monisha Khanna KapurAbstractDue to emergence of drug resistant pathogens, nearly all available medicines are becoming ineffective against these life threatening pathogens so there is dire need for the discovery of compounds having unique modes of action. During our previous studies, actinomycetes designated as 196 and RI.24 were isolated, screened for bioactive compounds production and characterized using 16S rRNA gene sequencing. Colony 196 was identified as strain of Strept...
Source: Microbiological Research - August 14, 2019 Category: Infectious Diseases Source Type: research

Phytoremediation of mine tailings by Brassica juncea inoculated with plant growth-promoting bacteria
Publication date: Available online 11 August 2019Source: Microbiological ResearchAuthor(s): José Carlos Mendoza-Hernández, Osmar Rodrigo Vázquez-Delgado, Maribel Castillo-Morales, Jenaro Leocadio Varela-Caselis, Juana Deisy Santamaría-Juárez, Octavio Olivares-Xometl, Janette Arriola Morales, Gabriela Pérez-OsorioAbstractMine tailings represent a serious environmental pollution problem and techniques such as phytoremediation using plant growth-promoting bacteria become an important solution due to their environmentally friendly nature. The study performed using Brassica juncea L. (I...
Source: Microbiological Research - August 12, 2019 Category: Infectious Diseases Source Type: research

Stress-induced adaptations in Salmonella: a ground for shaping its Pathogenesis
Publication date: Available online 11 August 2019Source: Microbiological ResearchAuthor(s): Diana Pradhan, Vidya Devi NegiAbstractMicroorganisms are able to adapt to multiple adverse environmental conditions that facilitate their survival. These microorganisms including bacteria, viruses, algae, fungi, and protozoans are exposed to different abiotic and biotic challenges throughout their life. Adaptations help these organisms to overcome the challenges and evolve as successful pathogens which at the same time might lead to severe disease outcome. The intracellular gram-negative pathogen Salmonella, the causative agent of t...
Source: Microbiological Research - August 12, 2019 Category: Infectious Diseases Source Type: research

MarTrack: A versatile toolbox of mariner transposon derivatives used for functional genetic analysis of bacterial genomes
In this study, we combined the merits of the random mutagenesis of mariner transposon and common efficient reporter marker genes or regulatory elements, mCherry, gfp, luxAB, lacZ, sacBR, and PBAD and antibiotic resistance cassettes to construct a series of derivative transposon vectors, pMmch, pMKGR, pMCGR, pMXKGR, pMLKGR, pMSGR, and pMPR, based on the initial transposon pMar2xT7. The function and effectiveness of the modified transposons were verified by introducing them into E. piscicida EIB202. Based on the toolbox, a transposon insertion mutant library containing approximately 3.0 × 105 distinct mut...
Source: Microbiological Research - August 9, 2019 Category: Infectious Diseases Source Type: research

Identification of plasmid encoded osmoregulatory genes from halophilic bacteria isolated from the rhizosphere of halophytes
Publication date: Available online 8 August 2019Source: Microbiological ResearchAuthor(s): Salma Mukhtar, Samia Ahmad, Aftab Bashir, Samina Mehnaz, Muhammad Sajjad Mirza, Kauser Abdulla MalikAbstractBacterial plasmids carry genes that code for additional traits such as osmoregulation, CO2 fixation, antibiotic and heavy metal resistance, root nodulation and nitrogen fixation. The main objective of the current study was to identify plasmid-conferring osmoregulatory genes in bacteria isolated from rhizospheric and non-rhizospheric soils of halophytes (Salsola stocksii and Atriplex amnicola). More than 55% of halophilic bacter...
Source: Microbiological Research - August 9, 2019 Category: Infectious Diseases Source Type: research

Regulatory rewiring through global gene regulations by PhoB and alarmone (p)ppGpp under various stress conditions
Publication date: Available online 5 August 2019Source: Microbiological ResearchAuthor(s): Varsha Jha, Nishant A. Dafale, Hemant J. PurohitAbstractThe phosphorus availability in soil ranged from
Source: Microbiological Research - August 7, 2019 Category: Infectious Diseases Source Type: research

Development of low-cost plant probiotic formulations of functional endophytes for sustainable cultivation of Coleus forskohlii
Publication date: Available online 5 August 2019Source: Microbiological ResearchAuthor(s): Anthati Mastan, Digeshwar Rane, Syed G. Dastager, C.S. Vivek BabuAbstractDeployment of plant endophytes at field level is reported to make an impact on agricultural crop productivity; development and deployment of suitable crop specific plant probiotics in a suitable delivery matrix is a value-added task. In our study, we attempted to develop bioformulations of native, fungal endophytes of Coleus forskohlii to improve plant yield using two different carrier-based materials (talc and wheat bran). Initially, fungal endophytes (RF1, SF1...
Source: Microbiological Research - August 7, 2019 Category: Infectious Diseases Source Type: research