Chapter 10 - Detection of microorganisms using recombinase polymerase amplification with lateral flow dipsticks
Publication date: 2020Source: Methods in Microbiology, Volume 47Author(s): Yuhang Zhang, Jinqiang Hu, Qingmei Li, Junqing Guo, Gaiping Zhang (Source: Methods in Microbiology)
Source: Methods in Microbiology - April 29, 2020 Category: Microbiology Source Type: research
Chapter 11 - Detection of microorganisms with lateral flow test strips
Publication date: 2020Source: Methods in Microbiology, Volume 47Author(s): Tatsuya Tominaga, Masaharu Ishii (Source: Methods in Microbiology)
Source: Methods in Microbiology - April 29, 2020 Category: Microbiology Source Type: research
Detection of microorganisms using recombinase polymerase amplification with lateral flow dipsticks
Publication date: Available online 23 April 2020Source: Methods in MicrobiologyAuthor(s): Yuhang Zhang, Jinqiang Hu, Qingmei Li, Junqing Guo, Gaiping Zhang (Source: Methods in Microbiology)
Source: Methods in Microbiology - April 24, 2020 Category: Microbiology Source Type: research
Immunological and molecular techniques used for determination of serotypes in Pasteurellaceae
Publication date: Available online 16 April 2020Source: Methods in MicrobiologyAuthor(s): Henrik Christensen, Janine Bossé, Øystein Angen, Niels Nørskov-Lauritsen, Magne Bisgaard (Source: Methods in Microbiology)
Source: Methods in Microbiology - April 17, 2020 Category: Microbiology Source Type: research
Introduction to immunological techniques in the clinical laboratory
Publication date: Available online 25 January 2020Source: Methods in MicrobiologyAuthor(s): Tony A. Slieman, Joerg LehesteAbstractThe field of immunology has exponentially expanded in the last few decades. This has much to do with the recent mapping effort of the human microbiome and discoveries of crosstalk, symbiosis, opportunism and disease. Along with a better understanding of the relationship between the human host and its microbiome, powerful immunodiagnostic techniques have been developed that have revolutionized clinical analysis laboratory medicine, including the study of microbial detection. In this introductory ...
Source: Methods in Microbiology - January 26, 2020 Category: Microbiology Source Type: research
Immunological detection of Zika virus: A summary in the context of general viral diagnostics
Publication date: Available online 20 January 2020Source: Methods in MicrobiologyAuthor(s): Rebecca Peters, Mario StevensonAbstractZika virus (ZIKV) captured mass public attention in 2014–16 due to its potentially devastating teratogenic effects in unborn babies. Although recent years show a decline in disease activity as ZIKV settles into endemic New World cycles, the significant challenges that originally complicated its diagnosis remain, and linkages to foetal microcephaly make safe, accurate, and timely detection especially important. The present chapter seeks to define current direct and indirect immunological detec...
Source: Methods in Microbiology - January 21, 2020 Category: Microbiology Source Type: research
Combining antigen detection and serology for the diagnosis of selected infectious diseases
Publication date: Available online 20 January 2020Source: Methods in MicrobiologyAuthor(s): Maria M. Plummer, Charles S. PaviaAbstractImmunoassay detection of specific antigens and host-produced antibodies directed against such antigens constitute two of the most widely used and successful methods for diagnosing infectious diseases and many of the non-infectious autoimmune disorders. We will be reviewing some of the epidemiology, microbiology, and clinical aspects of selected organisms and discuss their diagnosis by describing some of the major antigen detection systems and serologic techniques available to health care pro...
Source: Methods in Microbiology - January 21, 2020 Category: Microbiology Source Type: research
Development and improvement of a colony blot immunoassay for the detection of thermotolerant Campylobacter species
Publication date: Available online 17 January 2020Source: Methods in MicrobiologyAuthor(s): Hongsheng Huang, Philippe Raymond, Chris Grenier, Jessica FaheyAbstractCampylobacter species, particularly thermotolerant Campylobacter spp., such as C. jejuni, are major human foodborne pathogens worldwide. Culture methods are routinely used for the detection of this organism. Campylobacter-like colonies can be difficult to identify on agar plates overgrown with competitive bacteria. An alternative, simple and rapid confirmation testing without further tedious biochemical tests would be useful. Previously we developed a colony blot...
Source: Methods in Microbiology - January 18, 2020 Category: Microbiology Source Type: research
Detection of microorganisms with lateral flow test strips
Publication date: Available online 8 January 2020Source: Methods in MicrobiologyAuthor(s): Tatsuya Tominaga, Masaharu IshiiAbstractThe intake of foodborne pathogens causes serious health problems such as diarrhoea and vomiting. The spread of antibiotic-resistant bacteria, which often occurs via food, has becomes a global problem. Rapid detection of bacteria in food is crucial to prevent such fortuities. Lateral flow immunochromatographic assay (LFIA) has become popular as a method that can detect a target on-site using a strip. This chapter provides an overview of the recent research trends in LFIA, i.e., improvement of se...
Source: Methods in Microbiology - January 10, 2020 Category: Microbiology Source Type: research
Immunologic detection of Lyme disease and the related borrelioses
Publication date: Available online 31 December 2019Source: Methods in MicrobiologyAuthor(s): Charles S. PaviaAbstractLyme disease and the relapsing fever borrelioses are caused by spirochetal bacteria belonging to the genus Borrelia. They have a partially restricted worldwide distribution depending upon their species. They are transmitted to people following exposure to the following arthropod vectors: ticks (for Lyme disease and endemic relapsing fever) and lice (for epidemic relapsing fever). Soon after a cutaneous infection becomes established, these spirochetes disseminate via the haematogenous route and are capable of...
Source: Methods in Microbiology - January 1, 2020 Category: Microbiology Source Type: research
Immunological tests for diarrhoea caused by diarrhoeagenic Escherichia coli targeting their main virulence factors
Publication date: Available online 27 December 2019Source: Methods in MicrobiologyAuthor(s): Roxane M.F. Piazza, Bruna A. Caetano, Camila P. Henrique, Daniela Luz, Danielle D. Munhoz, Juliana M. Polatto, Letícia B. Rocha, Miriam A. Silva, Thais MitsunariAbstractGene detection does not assure the expression of the corresponding virulence factor. Therefore, the antibody-based assays comprise the largest group of rapid methods that can be employed for the detection of virulence factor production/secretion. For these antibody-based assays, polyclonal, monoclonal, or recombinant antibodies can be employed. Thus, this chapter p...
Source: Methods in Microbiology - December 29, 2019 Category: Microbiology Source Type: research
Serodiagnosis of Strongyloides stercoralis infection
Publication date: Available online 19 December 2019Source: Methods in MicrobiologyAuthor(s): Rahmah Noordin, Norsyahida Arifin, Dinesh Balachandra, Hussain AhmadAbstractStrongyloides stercoralis infection is an emerging healthcare problem worldwide due to the increasing number of migrant populations from endemic regions, transplantations, malignancies, and inflammatory conditions, coupled with advances in immunosuppressive therapies. The highly fatal hyperinfection should be prevented by testing at-risk people and symptomatic patients using reliable diagnostics. However, diagnosis of the infection is challenging since many...
Source: Methods in Microbiology - December 20, 2019 Category: Microbiology Source Type: research
Immunodetection of bacteria causing brucellosis
Publication date: Available online 11 December 2019Source: Methods in MicrobiologyAuthor(s): Sukhadeo B. Barbuddhe, Jess Vergis, Deepak B. RawoolAbstractBrucellosis, caused by Brucella species, is a neglected, under reported and under recognized infection of worldwide distribution. Owing to its greater economic and zoonotic importance, control of brucellosis is a priority target set forth by the WHO and development agencies. The genus Brucella is comprised of 12 species. The four main species infecting humans and livestock populations include B. melitensis, B. abortus, B. suis, and B. canis. Brucellosis is a cause of acute...
Source: Methods in Microbiology - December 12, 2019 Category: Microbiology Source Type: research
Series Page
Publication date: 2019Source: Methods in Microbiology, Volume 46Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - May 22, 2019 Category: Microbiology Source Type: research
Contributors
Publication date: 2019Source: Methods in Microbiology, Volume 46Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - May 22, 2019 Category: Microbiology Source Type: research
