Hemodynamic force is required for vascular smooth muscle cell recruitment to blood vessels during mouse embryonic development
This study reveals another role for hemodynamic force in regulating blood vessel development of the mouse, and opens up a new model to begin elucidating mechanotransduction pathways regulating vascular maturation.Graphical abstractIn normal flow embryos, vascular maturation occurs by the recruitment of vSMCs in the mesoderm to blood vessels exposed to high flow. This is followed by the attachment of the vSMCs to the high-flow vessels. In reduced-flow embryos, the vessels are exposed to lower flow, which minimizes the amount of recruited vSMCs towards any particular vessel. (Source: Mechanisms of Development)
Source: Mechanisms of Development - February 22, 2019 Category: Biology Source Type: research
Detecting coevolution of positively selected in turtles sperm-egg fusion proteins
Publication date: Available online 18 February 2019Source: Mechanisms of DevelopmentAuthor(s): Jingxiu Dong, Hui Jiang, Lei Xiong, Jiawei Zan, Jianjun Liu, Mengli Yang, Kai Zheng, Ziming Wang, Liuwang NieAbstractPhysically interacting sperm-egg proteins have been identified using gene-modified animals in some mammal species. Three proteins are essential for sperm-egg binding: Izumo1 on the sperm surface, and JUNO and CD9 on the egg surface. Most proteins linked to reproductive function evolve rapidly among species by positive selection, and have correlated evolutionary rates to compensate for changes on both the sperm and ...
Source: Mechanisms of Development - February 18, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: February 2019Source: Mechanisms of Development, Volume 155Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - January 31, 2019 Category: Biology Source Type: research
Xenopus slc7a5 is essential for notochord function and eye development
In this study, expression and function of slc7a5 were evaluated in Xenopus laevis embryos that develop without a placenta. Expression of slc7a5 was detected in the notochord and in the eye and it was not co-localized with slc3a2, which helps slc7a5 to localize at the plasma membrane, before the late neurula stage. Loss-of-function experiment with a morpholino antisense oligonucleotide led to defect of neural and non-neural patterning, inhibition of primary neurogenesis, and disruption of eye development. Disruption of neural development and primary neurogenesis was likely due to impaired notochord development as sonic hedg...
Source: Mechanisms of Development - January 7, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: December 2018Source: Mechanisms of Development, Volume 154Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - December 8, 2018 Category: Biology Source Type: research
MoD Special issue on “Developmental Biology in Latin America”
Publication date: December 2018Source: Mechanisms of Development, Volume 154Author(s): Juan R. Riesgo-Escovar, Roberto Mayor, Pablo Wappner, Irene Yan (Source: Mechanisms of Development)
Source: Mechanisms of Development - December 8, 2018 Category: Biology Source Type: research
MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin
In this study, we performed small RNA sequencing of a breast epithelial progenitor cell line (D492), and its mesenchymal derivative (D492M) cultured in three-dimensional microenvironment. Among the most downregulated miRNAs in D492M was miR-203a, a miRNA that plays an important role in epithelial differentiation. Increased expression of miR-203a was seen in D492, concomitant with increased complexity of branching. When miR-203a was overexpressed in D492M, a partial reversion towards epithelial phenotype was seen. Gene expression analysis of D492M and D492MmiR-203a revealed peroxidasin, a collagen IV cross-linker, as the mo...
Source: Mechanisms of Development - December 1, 2018 Category: Biology Source Type: research
Znfl1s are essential for patterning the anterior-posterior axis of zebrafish posterior hindbrain by acting as direct target genes of retinoic acid
Publication date: Available online 23 November 2018Source: Mechanisms of DevelopmentAuthor(s): Jingyun Li, Yingmin Zhao, Luqingqing He, Yun Huang, Xiaojing Yang, Lingling Yu, Qingshun Zhao, Xiaohua DongAbstractRA (retinoic acid) signaling is essential for the patterning the hindbrain of vertebrates. Although hundreds of potential RA targets genes are identified, the ones other than hox genes playing roles in patterning anterior-posterior axis of hindbrain by mediating RA signaling remains largely unknown. Previously, we reported that znfl1s play essential roles in the formation of posterior neuroectoderm in zebrafish embry...
Source: Mechanisms of Development - November 23, 2018 Category: Biology Source Type: research
Non-obstructive vas deferens and epididymis loss in cystic fibrosis rats
This study utilizes morphological and mechanistic endpoints to characterize the onset of bilateral atresia of the vas deferens in a recently derived cystic fibrosis (CF) rat model. Embryonic reproductive structures, including Wolffian (mesonephric) duct, Mullerian (paramesonephric) duct, mesonephric tubules, and gonad, were shown to mature normally through late embryogenesis, with involution of the vas deferens and/or epididymis typically occurring between birth and postnatal day 4 (P4), although timing and degree of atresia varied. No evidence of mucus obstruction, which is associated with pathology in other CF-affected t...
Source: Mechanisms of Development - November 3, 2018 Category: Biology Source Type: research
Cochlear connexin 30 homomeric and heteromeric channels exhibit distinct assembly mechanisms
Publication date: Available online 5 October 2018Source: Mechanisms of DevelopmentAuthor(s): Jean Defourny, Nicolas Thelen, Marc ThiryAbstractMany of the mutations in GJB2 and GJB6, which encode connexins 26 and 30 (Cx26 and Cx30), impair the formation of membrane channels and cause autosomal syndromic and non-syndromic hearing loss. In cochlear non-sensory supporting cells, Cx26 and Cx30 form two types of homomeric and heteromeric gap junctions. The biogenesis processes of these channels occurring in situ remain largely unknown. Here we show that Cx30 homomeric and Cx26/Cx30 heteromeric gap junctions exhibit distinct asse...
Source: Mechanisms of Development - October 5, 2018 Category: Biology Source Type: research
Cog4 is required for protrusion and extension of the epithelium in the developing semicircular canals
Publication date: Available online 1 October 2018Source: Mechanisms of DevelopmentAuthor(s): Aurélie Clément, Bernardo Blanco-Sánchez, Judy L. Peirce, Monte WesterfieldAbstractThe semicircular canals in the inner ear sense angular acceleration. In zebrafish, the semicircular canals develop from epithelial projections that grow toward each other and fuse to form pillars. The growth of the epithelial projections is driven by the production and secretion of extracellular matrix components by the epithelium. The conserved oligomeric Golgi 4 protein, Cog4, functions in retrograde vesicle transport within the Golgi and mutati...
Source: Mechanisms of Development - October 5, 2018 Category: Biology Source Type: research
Editorial Board
Publication date: October 2018Source: Mechanisms of Development, Volume 153Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - October 5, 2018 Category: Biology Source Type: research
Jitterbug/Filamin and Myosin-II form a complex in tendon cells required to maintain epithelial shape and polarity during musculoskeletal system development
Publication date: Available online 10 September 2018Source: Mechanisms of DevelopmentAuthor(s): Catalina Manieu, Gonzalo H. Olivares, Franco Vega-Macaya, Mauricio Valdivia, Patricio OlguínAbstractDuring musculoskeletal system development, mechanical tension is generated between muscles and tendon-cells. This tension is required for muscle differentiation and is counterbalanced by tendon-cells avoiding tissue deformation. Both, Jbug/Filamin, an actin-meshwork organizing protein, and non-muscle Myosin-II (Myo-II) are required to maintain the shape and cell orientation of the Drosophila notum epithelium during flight muscle ...
Source: Mechanisms of Development - September 11, 2018 Category: Biology Source Type: research
Evolutionary history of Mo25 gene in plants, a component of RAM/MOR signaling network
Publication date: Available online 9 September 2018Source: Mechanisms of DevelopmentAuthor(s): Fernanda M. Bizotto, Renan S. Ceratti, Antonio S.K. Braz, Hana Paula MasudaAbstractChange in cell morphogenesis is an important feature for proper development of eukaryotes. It is necessary for cell polarity and asymmetry and is essential for asymmetric cell division. RAM/MOR is a conserved signaling network that coordinates cell polarity determinants important for asymmetric cell division and cell polarity establishment. Mo25 is a scaffold protein that acts as a master regulator of the germinal center kinase (GCK) which triggers...
Source: Mechanisms of Development - September 9, 2018 Category: Biology Source Type: research
Roles of basal keratinocytes in actinotrichia formation
In this study, we elucidated the role of basal keratinocytes in actinotrichia formation. First, we developed the imaging tool that specifically visualizes the basal keratinocytes and actinotrichia. Then, we established the in vitro culture method of the basal keratinocytes and found that the keratinocytes developed fine needle-like structures in it. The TEM image of them showed the specific shadow pattern of actinotrichia, indicating that the fine needle-like structures are the newly made actinotrichia. Finally, we cultured the basal keratinocytes with mature actinotrichia and observed that the basal keratinocytes actively...
Source: Mechanisms of Development - September 6, 2018 Category: Biology Source Type: research
