Characterization of skn-1/wdr-23 phenotypes in Caenorhabditis elegans; pleiotrophy, aging, glutathione, and interactions with other longevity pathways
Publication date: July 2015 Source:Mechanisms of Ageing and Development, Volume 149 Author(s): Lanlan Tang , Keith P. Choe The SKN-1/Nrf transcription factors are master regulators of oxidative stress responses and are emerging as important determinants of longevity. We previously identified a protein named WDR-23 as a direct repressor of SKN-1 in C. elegans. Loss of wdr-23 influences stress resistance, longevity, development, and reproduction, but it is unknown if WDR-23 influences development and reproduction solely through SKN-1 and the mechanisms by which SKN-1 promotes stress resistance and longevity are poorly defi...
Source: Mechanisms of Ageing and Development - June 25, 2015 Category: Geriatrics Source Type: research

On the Complex Relationship between Energy Expenditure and Longevity: Reconciling the Contradictory Empirical Results with a Simple Theoretical Model
Publication date: Available online 15 June 2015 Source:Mechanisms of Ageing and Development Author(s): Chen Hou , Kaushalya Amunugama The relationship between energy expenditure and longevity has been a central theme in aging studies. Empirical studies have yielded controversial results, which cannot be reconciled by existing theories. In this paper, we present a simple theoretical model based on first principles of energy conservation and allometric scaling laws. The model takes into considerations the energy tradeoffs between life history traits and the efficiency of the energy utilization, and offers quantitative and ...
Source: Mechanisms of Ageing and Development - June 15, 2015 Category: Geriatrics Source Type: research

Genome-wide expression analyses of the stationary phase model of ageing in yeast
Publication date: Available online 12 June 2015 Source:Mechanisms of Ageing and Development Author(s): Kwanjeera Wanichthanarak , Nutvadee Wongtosrad , Dina Petranovic Ageing processes involved in replicative lifespan (RLS) and chronological lifespan (CLS) have been found to be conserved among many organisms, including in unicellular Eukarya such as yeast Saccharomyces cerevisiae. Here we performed an integrated approach of genome wide expression profiles of yeast at different time points, during growth and starvation. The aim of the study was to identify transcriptional changes in those conditions by using several diffe...
Source: Mechanisms of Ageing and Development - June 12, 2015 Category: Geriatrics Source Type: research

Could caveolae be acting as warnings of mitochondrial ageing?
Publication date: March 2015 Source:Mechanisms of Ageing and Development, Volumes 146–148 Author(s): Laura Caravia , Maria Dudau , Mihaela Gherghiceanu , Cristiana Tanase , Ana-Maria Enciu Ageing is a cellular process with many facets, some of which are currently undergoing a paradigm change. It is the case of “mitochondrial theory of ageing”, which, interestingly, has been found lately to cross paths with another ageing dysfunctional process – intracellular signalling – in an unexpected point (or place) – caveolae. The latter represent membrane microdomains altered in senescent cells,...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Relief of delayed oxidative stress by ascorbic acid can suppress radiation-induced cellular senescence in mammalian fibroblast cells
Publication date: March 2015 Source:Mechanisms of Ageing and Development, Volumes 146–148 Author(s): Shinko Kobashigawa , Genro Kashino , Hiromu Mori , Masami Watanabe Ionizing radiation-induced cellular senescence is thought to be caused by nuclear DNA damage that cannot be repaired. However, here we found that radiation induces delayed increase of intracellular oxidative stress after irradiation. We investigated whether the relief of delayed oxidative stress by ascorbic acid would suppress the radiation-induced cellular senescence in Syrian golden hamster embryo (SHE) cells. We observed that the level of oxidativ...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Gender- and region-dependent changes of redox biomarkers in the brain of successfully aging LOU/C rat.
Publication date: Available online 5 May 2015 Source:Mechanisms of Ageing and Development Author(s): Emmanuel Moyse , Madeleine Arseneault , Pierrette Gaudreau , Guylaine Ferland , Charles Ramassamy The Lou/C (LOU) rat is an obesity resistant strain with higher longevity and healthspan than common rats. The management of oxidative stress being important to successful aging, we characterized this process in the aging LOU rat. Male/female LOU rats were euthanized at 4, 20, 29 months. Macrodissected hippocampus, striatum, parietal cortex, cerebellum were assayed for tissue concentrations of glutathione (GSH), gamma-glutamyl...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Herbs to curb cyclic nucleotide phosphodiesterase and their potential role in Alzheimer’s disease
Publication date: Available online 4 June 2015 Source:Mechanisms of Ageing and Development Author(s): Ashwani Kumar , Vishavdeep Sharma , Vijay Pal Singh , Madhu Kaundal , Manish Kumar Gupta , Jitender Bariwal , Rahul Deshmukh Cyclic nucleotides viz., cAMP/cGMP has been well known to play important role in cellular function and deficiency in their levels has been implicated in the pathogenesis of various neurodegenerative disorders including Alzheimer’s disease (AD). Phosphodiesterases (PDE) are the enzymes involved in the metabolism of cyclic nucleotides and the inhibition of phosphodiesterases is considered to be...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Characterization of skn-1/wdr-23 phenotypes in Caenorhabditis elegans; pleitrophy, aging, glutathione, and interactions with other longevity pathways
Publication date: Available online 6 June 2015 Source:Mechanisms of Ageing and Development Author(s): Lanlan Tang , Keith P. Choe The SKN-1/Nrf transcription factors are master regulators of oxidative stress responses and are emerging as important determinants of longevity. We previously identified a protein named WDR-23 as a direct repressor of SKN-1 in C. elegans. Loss of wdr-23 influences stress resistance, longevity, development, and reproduction, but it is unknown if WDR-23 influences development and reproduction solely through SKN-1 and the mechanisms by which SKN-1 promotes stress resistance and longevity are poor...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Age-related gene expression changes in substantia nigra dopamine neurons of the rat
Publication date: Available online 9 June 2015 Source:Mechanisms of Ageing and Development Author(s): Gemma M. Parkinson , Christopher V. Dayas , Doug W. Smith Ageing affects most, if not all, functional systems in the body. For example, the somatic motor nervous system, responsible for initiating and regulating motor output to skeletal musculature, is vulnerable to ageing. The nigrostriatal dopamine pathway is one vulnerable component of this system with deficits in dopamine signalling contributing to major motor dysfunction, as exemplified in Parkinson’s disease (PD). However, while the dopamine deficit in PD is ...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Increased levels of p21(CIP1/WAF1) correlate with decreased chondrogenic differentiation potential in synovial membrane progenitor cells.
Publication date: July 2015 Source:Mechanisms of Ageing and Development, Volume 149 Author(s): Anand Oliveira Masson , Ricarda Hess , Kate O’Brien , Karri L. Bertram , Pankaj Tailor , Edward Irvine , Guomin Ren , Roman J. Krawetz Cartilage injuries are a major concern in the field of orthopedics. They occur following trauma, as well as from a variety of pathological conditions including Osteoarthritis (OA). Although cartilage does not exhibit robust endogenous repair, it has been demonstrated that modulating the activity of p21 can increase the regenerative abilities of cartilage in vitro and in vivo. Since the syn...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Functional variants in CYP1B1, KRAS and MTHFR genes are associated with shorter telomere length in postmenopausal women
Publication date: July 2015 Source:Mechanisms of Ageing and Development, Volume 149 Author(s): Jasmina Z. Cerne , Maja Pohar-Perme , Petra Cerkovnik , Ksenija Gersak , Srdjan Novakovic Estrogens and antioxidants indirectly alleviate telomere attrition. However, available clinical data on the association between hormone exposure and telomere length are inconclusive. In the present study, we examined the effects of exogenous estrogen use and of some genetic factors implicated in estrogen metabolism and oxidative stress response on mean leukocyte telomere length. We studied 259 postmenopausal women. Genotyping was conducted...
Source: Mechanisms of Ageing and Development - June 11, 2015 Category: Geriatrics Source Type: research

Novel ageing-biomarker discovery using data-intensive technologies
Publication date: Available online 6 June 2015 Source:Mechanisms of Ageing and Development Author(s): H.R. Griffiths , E.M. Augustyniak , S.J. Bennett , F. Debacq-Chainiaux , C.R. Dunston , P. Kristensen , C.J. Melchjorsen , Santos A. Navarrete , A. Simm , O. Toussaint Ageing is accompanied by many visible characteristics. Other biological and physiological markers are also well-described e.g. loss of circulating sex hormones and increased inflammatory cytokines. Biomarkers for healthy ageing studies are presently predicated on existing knowledge of ageing traits. The increasing availability of data-intensive methods ena...
Source: Mechanisms of Ageing and Development - June 7, 2015 Category: Geriatrics Source Type: research

MARK-AGE data management: Cleaning, exploration and visualization of data
Publication date: Available online 21 May 2015 Source:Mechanisms of Ageing and Development Author(s): Jennifer Baur , Maria Moreno-Villanueva , Tobias Kötter , Thilo Sindlinger , Alexander Bürkle , Michael R. Berthold , Michael Junk Databases are an organized collection of data and necessary to investigate a wide spectrum of research questions. For data evaluation analyzers should be aware of possible data quality problems that can compromise results validity. Therefore data cleaning is an essential part of the data management process, which deals with the identification and correction of errors in order to imp...
Source: Mechanisms of Ageing and Development - June 5, 2015 Category: Geriatrics Source Type: research

The MARK-AGE extended database: data integration and pre-processing
Publication date: Available online 21 May 2015 Source:Mechanisms of Ageing and Development Author(s): J. Baur , T. Kötter , M. Moreno-Villanueva , T. Sindlinger , M.R. Berthold , A. Bürkle , M. Junk MARK-AGE is a recently completed European population study, where, bioanalytical and anthropometric data were collected from human subjects at a large scale. To facilitate data analysis and mathematical modelling, an extended database had to be constructed, integrating the data sources that were part of the project. This step involved checking, transformation and documentation of data. The success of downstream anal...
Source: Mechanisms of Ageing and Development - June 5, 2015 Category: Geriatrics Source Type: research

Metformin inhibits age-related centrosome amplification in Drosophila midgut stem cells through AKT/TOR pathway
Publication date: Available online 16 May 2015 Source:Mechanisms of Ageing and Development Author(s): Hyun-Jin Na , Joung-Sun Park , Jung-Hoon Pyo , Ho-Jun Jeon , Young-Shin Kim , Robert Arking , Mi-Ae Yoo We delineated the mechanism regulating the inhibition of centrosome amplification by metformin in Drosophila intestinal stem cells (ISCs). Age-related changes in tissue-resident stem cells may be closely associated with tissue aging and age-related diseases, such as cancer. Centrosome amplification is a hallmark of cancers. Our recent work showed that Drosophila ISCs are an excellent model for stem cell studies evaluat...
Source: Mechanisms of Ageing and Development - May 17, 2015 Category: Geriatrics Source Type: research

Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium
This study investigated whether increased levels of activated c-jun N-terminal kinase (JNK) is responsible for the decline of Cx43 during ageing. Right atria from guinea pigs aged between 1 day and 38 months of age were examined. The area of the intercalated disc increased with age concurrent with a 75% decline in C43 protein expression. An age-dependent increase in activated-JNK correlated with a rise in phosphorylated Cx43, but also slowing of action potential conduction velocity across the atria from 0.38±0.01m/s at 1 month of age to 0.30±0.01m/s at 38 months. The JNK activator anisomycin increased activat...
Source: Mechanisms of Ageing and Development - May 8, 2015 Category: Geriatrics Source Type: research

PARP-1 involvement in neurodegeneration: A focus on Alzheimer’s and Parkinson’s diseases
Publication date: March 2015 Source:Mechanisms of Ageing and Development, Volumes 146–148 Author(s): Sara Martire , Luciana Mosca , Maria d’Erme DNA damage is the prime activator of the enzyme poly(ADP-ribose)polymerase1 (PARP-1) whose overactivation has been proven to be associated with the pathogenesis of numerous central nervous system disorders, such as ischemia, neuroinflammation, and neurodegenerative diseases. Under oxidative stress conditions PARP-1 activity increases, leading to an accumulation of ADP-ribose polymers and NAD+ depletion, that induces energy crisis and finally cell death. This review a...
Source: Mechanisms of Ageing and Development - April 28, 2015 Category: Geriatrics Source Type: research

Caloric restriction, resveratrol and melatonin: Role of SIRT1 and implications for aging and related-diseases
Publication date: March 2015 Source:Mechanisms of Ageing and Development, Volumes 146–148 Author(s): Margarita R. Ramis , Susana Esteban , Antonio Miralles , Dun-Xian Tan , Russel J. Reiter Aging is an inevitable and multifactorial biological process. Free radicals have been implicated in aging processes; it is hypothesized that they cause cumulative oxidative damage to crucial macromolecules and are responsible for failure of multiple physiological mechanisms. However, recent investigations have also suggested that free radicals can act as modulators of several signaling pathways such as those related to sirtuins....
Source: Mechanisms of Ageing and Development - April 11, 2015 Category: Geriatrics Source Type: research

MARK-AGE population: From the human model to new insights
Publication date: Available online 2 April 2015 Source:Mechanisms of Ageing and Development Author(s): Miriam Capri , María Moreno-Villanueva , Elisa Cevenini , Elisa Pini , Maria Scurti , Vincenzo Borelli , Maria Giustina Palmas , Marco Zoli , Christiane Schön , Anne Siepelmeyer , Jürgen Bernhardt , Simone Fiegl , Gerben Zondag , Anton J.M. de Craene , Antti Hervonen , Mikko Hurme , Ewa Sikora , Efstathios S. Gonos , Konstantinos Voutetakis , Olivier Toussaint , Florence Debacq-Chainiaux , Beatrix Grubeck-Loebenstein , Alexander Bürkle , Claudio Franceschi (Source: Mechanisms of Ageing and Development)
Source: Mechanisms of Ageing and Development - April 11, 2015 Category: Geriatrics Source Type: research

NRMT1 knockout mice exhibit phenotypes associated with impaired DNA repair and premature aging
Publication date: Available online 2 April 2015 Source:Mechanisms of Ageing and Development Author(s): Lindsay A. Bonsignore , John G. Tooley , Patrick M. Van Hoose , Eugenia Wang , Alan Cheng , Marsha P. Cole , Christine E. Schaner Tooley Though defective genome maintenance and DNA repair have long been known to promote phenotypes of premature aging, the role, protein methylation plays in these processes is only now emerging. We have recently identified the first N-terminal methyltransferase, NRMT1, which regulates protein-DNA interactions and is necessary for both accurate mitotic division and nucleotide excision repai...
Source: Mechanisms of Ageing and Development - April 11, 2015 Category: Geriatrics Source Type: research

Activation-induced and damage-induced cell death in aging human T cells
Publication date: Available online 2 April 2015 Source:Mechanisms of Ageing and Development Author(s): Ewa Sikora In multicellular organisms the proper system functionality is ensured by the balance between cell division, differentiation, senescence and death. This balance is changed during aging. Immunosenescence plays a crucial role in aging and leads to the shrinkage of T cell repertoire and the propensity to apoptosis. The elimination of expanded T cells at the end of immune response is crucial to maintain homeostasis and avoid any uncontrolled inflammation. Resting mature T lymphocytes, when activated via their anti...
Source: Mechanisms of Ageing and Development - April 11, 2015 Category: Geriatrics Source Type: research

Protein modification and maintenance systems as biomarkers of ageing
Publication date: Available online 3 April 2015 Source:Mechanisms of Ageing and Development Author(s): Valerie Vanhooren , Alexander Navarrete Santos , Konstantinos Voutetakis , Isabelle Petropoulos , Claude Libert , Andreas Simm , Efstathios S. Gonos , Bertrand Friguet Changes in the abundance and post-translational modification of proteins and accumulation of some covalently modified proteins have been proposed to represent hallmarks of biological ageing. Within the frame of the Mark-Age project, the workpackage dedicated to “markers based on proteins and their modifications” has been firstly focused on enz...
Source: Mechanisms of Ageing and Development - April 3, 2015 Category: Geriatrics Source Type: research

Molecular responses in the telomere-mitochondrial axis of ageing in the elderly: A candidate gene approach
Publication date: January 2015 Source:Mechanisms of Ageing and Development, Volume 145 Author(s): Nicky Pieters , Bram G. Janssen , Linda Valeri , Bianca Cox , Ann Cuypers , Harrie Dewitte , Michelle Plusquin , Karen Smeets , Tim S. Nawrot Experimental evidence shows that telomere shortening induces mitochondrial damage but so far studies in humans are scarce. Here, we investigated the association between leukocyte telomere length (LTL) and mitochondrial DNA (mtDNA) content in elderly and explored possible intermediate mechanisms by determining the gene expression profile of candidate genes in the telomere-mitochondrial ...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

Aging induced cortical drive alterations during sleep in rats
Publication date: March 2015 Source:Mechanisms of Ageing and Development, Volumes 146–148 Author(s): Jelena Ciric , Katarina Lazic , Jelena Petrovic , Aleksandar Kalauzi , Jasna Saponjic We followed the impact of healthy aging on cortical drive during sleep in rats by using the corticomuscular coherence (CMC). We employed the chronic electrodes implantation for sleep recording in adult, male Wistar rats, and followed the aging impact during sleep from 3 to 5.5 months age. We have analyzed the sleep/wake states architecture, and the sleep/wake state related EEG microstructure and CMCs. We evidenced the topographical...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

The different expression profiles of microRNAs in elderly and young human dental pulp and the role of miR-433 in human dental pulp cells
Publication date: March 2015 Source:Mechanisms of Ageing and Development, Volumes 146–148 Author(s): Kejing Wang , Li Li , Junjun Wu , Qin Qiu , Fengjuan Zhou , Hongkun Wu As a kind of endogenous noncoding small RNA, MicroRNA (miRNA) plays important roles of regulation to various physiological functions, while its affections on senescence of human dental pulp cell (HDPCs) are still unknown. Thus, we identified the senescence-associated miRNAs in HDPCs by microarray analysis, predicted their targets and regulatory signaling pathway by gene ontology and Kyoto encyclopedia of genes and genomes pathway database analysi...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

Oxidized LDL and NO synthesis—Biomarkers of endothelial dysfunction and ageing
Publication date: Available online 21 March 2015 Source:Mechanisms of Ageing and Development Author(s): Daniela Gradinaru , Claudia Borsa , Cristina Ionescu , Gabriel Ioan Prada Oxidized LDL (oxLDL) and nitric oxide (NO) exert contradictory actions within the vascular endothelium microenvironment influencing key events in atherogenesis. OxLDL and NO are so far regarded as representative parameters of oxidative stress and endothelial dysfunction, new targets in prevention, diagnosis and therapy of cardiovascular diseases, and also as candidate biomarkers in evaluating the human biological age. The aim of this review is to...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

Conservation of pro-longevity genes among mammals
Publication date: Available online 21 March 2015 Source:Mechanisms of Ageing and Development Author(s): Carter M. Lindborg , Kathleen J. Propert , Robert J. Pignolo Genes which confer a relative longevity advantage may be regulated at the level of transcription or translation. Alternatively, pro-longevity genes may mediate their effects at the level of protein structure-functional relationships that are beneficially optimized in long-lived species. Longevity associated genes (LAGs) may be operationally defined as genes that confer beneficial effects and are relatively more conserved among long-lived species. Global and l...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

MARK-AGE Standard Operating Procedures (SOPs): a successful effort
Publication date: Available online 26 March 2015 Source:Mechanisms of Ageing and Development Author(s): María Moreno-Villanueva , Miriam Capri , Nicolle Breusing , Anne Siepelmeyer , Federica Sevini , Alessandro Ghezzo , Anton J.M.de Craen , Antti Hervonen , Mikko Hurme , Christiane Schön , Tilman Grune , Claudio Franceschi , Alexander Bürkle Within the MARK-AGE project, a population study (3337 subjects) was conducted to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any single marker. The MARK-AGE project...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

The MARK-AGE Phenotypic Database: Structure and Strategy
Publication date: Available online 27 March 2015 Source:Mechanisms of Ageing and Development Author(s): María Moreno-Villanueva , Tobias Kötter , Thilo Sindlinger , Jennifer Baur , Sebastian Oehlke , Alexander Bürkle , Michael R. Berthold In the context of the MARK-AGE study, anthropometric, clinical and social data as well as samples of venous blood, buccal mucosal cells and urine were systematically collected from 3337 volunteers. Information from about 500 standardized questions and about 500 analyzed biomarkers needed to be documented per individual. On the one hand handling with such a vast amount o...
Source: Mechanisms of Ageing and Development - March 27, 2015 Category: Geriatrics Source Type: research

MARK-AGE Biomarkers of Ageing
Publication date: Available online 24 March 2015 Source:Mechanisms of Ageing and Development Author(s): Alexander Bürkle , María Moreno-Villanueva , Jürgen Bernhard , María Blasco , Gerben Zondag , Jan Hoeijmakers , Olivier Toussaint , Beatrix Grubeck-Loebenstein , Eugenio Mocchegiani , Sebastiano Collino , Sthatis Gonos , Ewa Sikora , Daniela Gradinaru , Martijn Dolle , Michel Salmon , Peter Kristensen , Helen Griffiths , Claude Libert , Tilman Grune , Nicolle Breusing , Andreas Simm , Claudio Franceschi , Miriam Capri , Duncan Talbot , Paola Caiafa , Bertrand Friguet , Eline Slagboom , Antti Her...
Source: Mechanisms of Ageing and Development - March 24, 2015 Category: Geriatrics Source Type: research

Reconfiguration of DNA methylation in aging
Publication date: Available online 20 February 2015 Source:Mechanisms of Ageing and Development Author(s): Michele Zampieri , Fabio Ciccarone , Roberta Calabrese , Claudio Franceschi , Alexander Bürkle , Paola Caiafa A complex interplay between multiple biological effects shapes the aging process. The advent of genome-wide quantitative approaches in the epigenetic field has highlighted the effective impact of epigenetic deregulation, particularly of DNA methylation, on aging. Age-associated alterations in DNA methylation are commonly grouped in the phenomenon known as “epigenetic drift” which is characte...
Source: Mechanisms of Ageing and Development - February 24, 2015 Category: Geriatrics Source Type: research

Reconfiguration of DNA methylation in ageing
Publication date: Available online 20 February 2015 Source:Mechanisms of Ageing and Development Author(s): Michele Zampieri , Fabio Ciccarone , Roberta Calabrese , Claudio Franceschi , Alexander Bürkle , Paola Caiafa A complex interplay between multiple biological effects shapes the ageing process. The advent of genome-wide quantitative approaches in the epigenetic field has highlighted the effective impact of epigenetic deregulation, particularly of DNA methylation, on ageing. Age-associated alterations in DNA methylation are commonly grouped in the phenomenon known as “epigenetic drift” which is charac...
Source: Mechanisms of Ageing and Development - February 20, 2015 Category: Geriatrics Source Type: research

Osteoporosis: from osteoscience to neuroscience and beyond
Publication date: Available online 11 February 2015 Source:Mechanisms of Ageing and Development Author(s): Ping Kwan Osteoporosis is well known to be a poly-factorial skeletal disorder characterized by a low bone mineral density (BMD) at which the risk of developing fracture is remarkably increased and affecting both the quality and quantity of life. Although it is nearly 180 years since its first pathological identification, there is no effective cure against such ageing-associated health concern. Traditional research direction on osteoporosis was mainly focused on the balance between bone formation and resorption, in w...
Source: Mechanisms of Ageing and Development - February 13, 2015 Category: Geriatrics Source Type: research

Age and genetic determinants of variation of circulating levels of the receptor for advanced glycation end products (RAGE) in the general human population
We examined the contribution of age, body composition, and putative genetic sources to the interindividual variation of sRAGE. Its plasma levels were measured in 1557 apparently healthy individuals from 359 nuclear families. Statistical analysis revealed that all the aforementioned factors are statistically significantly associated with sRAGE levels. The levels of sRAGE consistently decreased with age (R=-0.264, p=<0.001) and with the indices of obesity, such as BMI. However, of special interest was the highly significant and previously not reported independent correlation with fat free mass (p<0.001). The pu...
Source: Mechanisms of Ageing and Development - February 13, 2015 Category: Geriatrics Source Type: research

Comparison of telomere length between population-specific mitochondrial haplogroups among different age groups in a Latvian population
In conclusion this study indicates that specific mitochondrial DNA hgs do not have a significant, if any, influence on the variation of TL in Latvians. (Source: Mechanisms of Ageing and Development)
Source: Mechanisms of Ageing and Development - February 8, 2015 Category: Geriatrics Source Type: research

Acetyl-l-carnitine increases mitochondrial protein acetylation in the aged rat heart
Publication date: Available online 7 February 2015 Source:Mechanisms of Ageing and Development Author(s): Janos Kerner , Elizabeth Yohannes , Kwangwon Lee , Ashraf Virmani , Aleardo Koverech , Claudio Cavazza , Mark R. Chance , Charles Hoppel Previously we showed that in vivo treatment of elderly Fisher 344 rats with acetylcarnitine abolished the age-associated defect in respiratory chain complex III in interfibrillar mitochondria and improved the functional recovery of the ischemic/reperfused heart. Herein, we explored mitochondrial protein acetylation as a possible mechanism for acetylcarnitine’s effect. In vivo ...
Source: Mechanisms of Ageing and Development - February 8, 2015 Category: Geriatrics Source Type: research

Serum copper to zinc ratio: Relationship with aging and health status
Publication date: Available online 7 February 2015 Source:Mechanisms of Ageing and Development Author(s): Marco Malavolta , Francesco Piacenza , Andrea Basso , Robertina Giacconi , Laura Costarelli , Eugenio Mocchegiani The serum concentrations of copper (Cu) and zinc (Zn) are strictly regulated by compensatory mechanisms that act to stabilize them within certain ranges of nutritional intake. However, there are mechanisms that are built to decrease serum concentration of Zn and to increase serum concentration of Cu in the presence of inflammatory conditions, so that a common feature of several age-related chronic disease...
Source: Mechanisms of Ageing and Development - February 8, 2015 Category: Geriatrics Source Type: research

Histone methylation codes involved in stemness, multipotency, and senescence in budding tunicates
We examined the dynamics of nuclear histone H3 trimethylation related to cell differentiation and aging in a budding tunicate, Polyandrocarpa misakiensis. Throughout zooidal life, multipotent epithelial and coelomic cell nuclei showed strong trimethylation signals at H3 lysine27 (H3K27me3), consistent with the results of western blotting. Epidermal H3K27me3 repeatedly appeared in protruding buds and disappeared in senescent adult zooids. The budding-specific cytostatic factor TC14-3 allowed aging epidermal cells to restore H3K27me3 signals and mitochondrial gene activities via mitochondrial transcription factor a, all of w...
Source: Mechanisms of Ageing and Development - January 12, 2015 Category: Geriatrics Source Type: research

Sphingolipid metabolism regulates development and lifespan in Caenorhabditis elegans
Publication date: Available online 28 November 2014 Source:Mechanisms of Ageing and Development Author(s): Roy G. Cutler , Kenneth W. Thompson , Simonetta Camandola , Kendra T. Mack , Mark P. Mattson Sphingolipids are a highly conserved lipid component of cell membranes involved in the formation of lipid raft domains that house many of the receptors and cell-to-cell signaling factors involved in regulating cell division, maturation, and terminal differentiation. By measuring and manipulating sphingolipid metabolism using pharmacological and genetic tools in Caenorhabditis elegans, we provide evidence that the synthesis a...
Source: Mechanisms of Ageing and Development - November 29, 2014 Category: Geriatrics Source Type: research

Circulating miR-21, miR-146a and Fas ligand respond to postmenopausal estrogen-based hormone replacement therapy – a study with monozygotic twin pairs
Publication date: Available online 28 November 2014 Source:Mechanisms of Ageing and Development Author(s): Reeta Kangas , Eija Pöllänen , Maria Rita Rippo , Catia Lanzarini , Francesco Prattichizzo , Paula Niskala , Juulia Jylhävä , Sarianna Sipilä , Jaakko Kaprio , Antonio Domenico Procopio , Miriam Capri , Claudio Franceschi , Fabiola Olivieri , Vuokko Kovanen Biological aging is associated with physiological deteriorations, which are partly due to changes in the hormonal profile. MicroRNAs regulate various processes associated with cell senescence; differentiation, replication and apoptosis. S...
Source: Mechanisms of Ageing and Development - November 29, 2014 Category: Geriatrics Source Type: research

Stem cells and aging in the hematopoietic system
Publication date: January–February 2009 Source:Mechanisms of Ageing and Development, Volume 130, Issues 1–2 Author(s): Luigi A. Warren , Derrick J. Rossi The effector cells of the blood have limited lifetimes and must be replenished continuously throughout life from a small reserve of hematopoietic stem cells (HSCs) in the bone marrow. Although serial bone marrow transplantation experiments in mice suggest that the replicative potential of HSCs is finite, there is little evidence that replicative senescence causes depletion of the stem cell pool during the normal lifespan of either mouse or man. Studies condu...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Transgenerational endocrine pancreatic adaptation in mice from maternal protein restriction in utero
Publication date: March 2011 Source:Mechanisms of Ageing and Development, Volume 132, Issue 3 Author(s): Eliete Dalla Corte Frantz , Marcia Barbosa Aguila , Alessandra da Rocha Pinheiro-Mulder , Carlos Alberto Mandarim-de-Lacerda Exposure of pregnant mice to a low-protein diet (LP) impairs endocrine pancreas development in their offspring. There is evidence that this phenomenon may persist in subsequent generations. Here, we evaluated the effect of LP on glucose metabolism and pancreatic morphometry in the F3 offspring of mice at birth and weaning. LP pups in the first generation were smaller at birth, but catch-up growt...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Multiple suppression pathways of canonical Wnt signalling control thymic epithelial senescence
Publication date: May 2011 Source:Mechanisms of Ageing and Development, Volume 132, Issue 5 Author(s): Zoltan Varecza , Krisztian Kvell , Gergely Talabér , Gyorgy Miskei , Veronika Csongei , Domokos Bartis , Graham Anderson , Eric J. Jenkinson , Judit E. Pongracz Members of the Wnt family of secreted glyco-lipo-proteins affect intrathymic T-cell development and are abundantly secreted by thymic epithelial cells (TECs) that create the specific microenvironment for thymocytes to develop into mature T-cells. During ageing, Wnt expression declines allowing adipoid involution of the thymic epithelium leading to reduced...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Insulin-like growth factor binding protein-6 delays replicative senescence of human fibroblasts
Publication date: October 2011 Source:Mechanisms of Ageing and Development, Volume 132, Issue 10 Author(s): Lucia Micutkova , Thomas Diener , Chen Li , Adelina Rogowska-Wrzesinska , Christoph Mueck , Eveline Huetter , Birgit Weinberger , Beatrix Grubeck-Loebenstein , Peter Roepstorff , Rong Zeng , Pidder Jansen-Duerr Cellular senescence can be induced by a variety of mechanisms, and recent data suggest a key role for cytokine networks to maintain the senescent state. Here, we have used a proteomic LC-MS/MS approach to identify new extracellular regulators of senescence in human fibroblasts. We identified 26 extracellular...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Localised micro-mechanical stiffening in the ageing aorta
Publication date: October 2011 Source:Mechanisms of Ageing and Development, Volume 132, Issue 10 Author(s): Helen K. Graham , Riaz Akhtar , Constantinos Kridiotis , Brian Derby , Tribikram Kundu , Andrew W. Trafford , Michael J. Sherratt Age-related loss of tissue elasticity is a common cause of human morbidity and arteriosclerosis (vascular stiffening) is associated with the development of both fatal strokes and heart failure. However, in the absence of appropriate micro-mechanical testing methodologies, multiple structural remodelling events have been proposed as the cause of arteriosclerosis. Therefore, using a model ...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Short bouts of mild-intensity physical exercise improve spatial learning and memory in aging rats: Involvement of hippocampal plasticity via AKT, CREB and BDNF signaling
Publication date: November–December 2011 Source:Mechanisms of Ageing and Development, Volume 132, Issues 11–12 Author(s): Aderbal S. Aguiar Jr. , Adalberto A. Castro , Eduardo L. Moreira , Viviane Glaser , Adair R.S. Santos , Carla I. Tasca , Alexandra Latini , Rui D.S. Prediger In the present study, we investigated whether mild-intensity physical exercise represents a successful strategy to enhance spatial learning and memory and hippocampal plasticity in aging rats, as previously described for long-term exposure to running wheel or treadmill exercise. Aging Wistar rats were submitted to short bouts (4&ndash...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Dietary restriction increases skeletal muscle mitochondrial respiration but not mitochondrial content in C57BL/6 mice
We examined proteins and mRNAs associated with mitochondrial biogenesis and measured mitochondrial respiration in permeabilised myofibres using high resolution respirometry. 30% DR, irrespective of duration, had no effect on citrate synthase activity. In contrast, total and nuclear protein levels of PGC-1α, mRNA levels of several mitochondrial associated proteins (Pgc-1α, Nrf1, Core 1, Cox IV, Atps) and cytochrome c oxidase content were increased in skeletal muscle of DR mice. Furthermore, a range of mitochondrial respiration rates were increased significantly by DR, with DR partially attenuating the age-relate...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Manipulation of in vivo iron levels can alter resistance to oxidative stress without affecting ageing in the nematode C. elegans
Publication date: May 2012 Source:Mechanisms of Ageing and Development, Volume 133, Issue 5 Author(s): Sara Valentini , Filipe Cabreiro , Daniel Ackerman , Muhammed M. Alam , Micha B.A. Kunze , Christopher W.M. Kay , David Gems Iron-catalyzed generation of free radicals leads to molecular damage in vivo, and has been proposed to contribute to organismal ageing. Here we investigate the role of free iron in ageing in the nematode Caenorhabditis elegans. Media supplementation with Fe(III) increased free iron levels in vivo, as detected by continuous-wave electron paramagnetic resonance spectroscopy and elevated expression o...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Frequency distribution analysis of activation times and regional fibrosis in murine Scn5a+/− hearts: The effects of ageing and sex
This study assessed for contributions of such processes to the mechanism of conduction changes in Scn5a +/− and WT hearts stratified by age (3 and 12 months) and sex. In vivo electrocardiographic chest-lead assessment demonstrated greater incidences of bundle branch block in all Scn5a +/− mice compared to WT. Frequency analysis of right ventricular (RV) epicardial activation obtained from a 64-channel multi-electrode array demonstrated greater prominence of late conducting components in Scn5a +/− compared to WT male, and in male compared to female Scn5a +/− following stratification by genotype and s...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research

Assessment of candidate ocular biomarkers of ageing in a South African adult population: Relationship with chronological age and systemic biomarkers
Publication date: July–August 2013 Source:Mechanisms of Ageing and Development, Volume 134, Issues 7–8 Author(s): Sophia Pathai , Clare E. Gilbert , Stephen D. Lawn , Helen A. Weiss , Tunde Peto , Colin Cook , Tien Y. Wong , Paul G. Shiels Certain anatomic and functional parameters of the eye change with increasing chronological age. They may, therefore, serve as potential biomarkers of ageing. We investigated associations between four such ocular parameters (lens density, retinal vessel calibre, corneal endothelial cells and retinal nerve fibre layer thickness) and two ‘cellular’ biomarkers of ag...
Source: Mechanisms of Ageing and Development - November 7, 2014 Category: Geriatrics Source Type: research