Journal of Tissue Engineering and Regenerative Medicine This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

3D matrix ‐embedding inhibits cycloheximide‐mediated sensitization to TNF‐alpha‐induced apoptosis of human endothelial cells
Abstract The programmed form of cell death (apoptosis) is essential for normal development of multicellular organisms. Dysregulation of apoptosis has been linked with embryonal death and is involved in the pathophysiology of various diseases. Others and we previously demonstrated endothelial biology being intertwined with biochemical and structural composition of the subendothelial basement membrane. We now demonstrate that a three‐dimensional growing environment significantly shields endothelial cells from cytokine‐induced apoptosis. Detailed analysis reveals differences in intracellular signaling pathways in naive en...
Source: Journal of Tissue Engineering and Regenerative Medicine - December 3, 2017 Category: Biotechnology Authors: Michael Saemisch, Markus Nickmann, Lisa Riesinger, Elazer R. Edelman, Heiko Methe Tags: RESEARCH ARTICLE Source Type: research

Optimized human platelet lysate as novel basis for a serum ‐, xeno‐, and additive‐free corneal endothelial cell and tissue culture
Abstract The expansion of donor‐derived corneal endothelial cells (ECs) is a promising approach for regenerative therapies in corneal diseases. To achieve the best Good Manufacturing Practice standard the entire cultivation process should be devoid of nonhuman components. However, so far, there is no suitable xeno‐free protocol for clinical applications. We therefore introduce a processed variant of a platelet lysate for the use in corneal cell and tissue culture based on a Good Manufacturing Practice‐grade thrombocyte concentrate. This processed human platelet lysate (phPL), free of any animal components and of anti...
Source: Journal of Tissue Engineering and Regenerative Medicine - December 3, 2017 Category: Biotechnology Authors: Daniel Thieme, Lynn Reuland, Toni Lindl, Friedrich Kruse, Thomas Fuchsluger Tags: RESEARCH ARTICLE Source Type: research

Four and a half domain 2 (FHL2) scaffolding protein is a marker of connective tissues of developing digits and regulates fibrogenic differentiation of limb mesodermal progenitors.
Summary FHL2 is a multifunctional scaffolding protein of well‐known function regulating cell signaling cascades and gene transcription in cancer tissues. However, its function in embryonic systems is poorly characterized. Here, we show that Fhl2 is involved in the differentiation of connective tissues of developing limb autopod. We show that Fhl2 exhibits spatially restricted and temporally dynamic expression around the tendons of developing digits, interphalangeal joint capsules, and fibrous peridigital tissue. Immunolabeling analysis of the skeletal progenitors identified a predominant, but not exclusive, cytoplasmic d...
Source: Journal of Tissue Engineering and Regenerative Medicine - December 1, 2017 Category: Biotechnology Authors: C.I. Lorda ‐Diez, J.A. Montero, C. Sanchez‐Fernandez, J.A. Garcia‐Porrero, J. Chimal‐Monroy, J.M. Hurle Tags: RESEARCH ARTICLE Source Type: research

In vivo migration of endogenous brain progenitor cells guided by an injectable peptide amphiphile biomaterial
Abstract Biomaterials hold great promise in helping the adult brain regenerate and rebuild after trauma. Peptide amphiphiles (PA) are highly versatile biomaterials, gelling and forming macromolecular structures when exposed to physiological levels of electrolytes. We are here reporting on the first ever in vivo use of self‐assembling peptide amphiphile carrying a Tenascin‐C signal (E2Ten‐C PA) for the re‐direction of endogenous neuroblasts in the rodent brain. The PA forms highly aligned nanofibers, displaying the migratory sequence of Tenascin‐C glycoprotein as epitope. In this in vivo work, we have formed in si...
Source: Journal of Tissue Engineering and Regenerative Medicine - December 1, 2017 Category: Biotechnology Authors: Reza Motalleb, Eric J. Berns, Piyush Patel, Julie Gold, Samuel I. Stupp, H. Georg Kuhn Tags: RESEARCH ARTICLE Source Type: research

Effect of Patterned Electrospun Hierarchical Structures on Alignment and Differentiation of Mesenchymal Stem Cells: Biomimicking Bone
Abstract Considering the complex hierarchical structure of bone, biomimicking the micro and nano level features should be an integral part of scaffold fabrication for successful bone regeneration. We aim to biomimic the micro‐ and nano‐structure of bone and study the effect of physical cues on cell alignment, proliferation and differentiation. To achieve this, we have divided the scaffolds into groups: electrospun SU‐8 nanofibers, electrospun SU‐8 nanofibers with UV treatment and micropatterned (20 μm sized ridges and grooves) SU‐8 nanofibers by photolithography with UV treatment. Two types of culture conditions...
Source: Journal of Tissue Engineering and Regenerative Medicine - December 1, 2017 Category: Biotechnology Authors: Sharanya Sankar, Manohar Kakunuri, Sindhuja D. E, Chandra.S. Sharma, Subha.N. Rath Tags: RESEARCH ARTICLE Source Type: research