Journal of Pharmacological and Toxicological Methods This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A natural complex product Yaocha reduces uric acid level in a live zebrafish model
This study was aimed to assess uric acid (UA)-lowering effect and its possible mechanisms of a natural complex product Yaocha in a live zebrafish model. Methods: The zebrafish high UA model was established by feeding 5 dpf zebrafish with both an uricase inhibitor potassium oxonate at 10 mM and an UA synthesis precursor xanthine sodium at 0.5 mM for 24 h.. Yaocha was administered to the high UA zebrafish through soaking at 3 various concentrations, with allopurinol as a positive control. UA level, xanthine oxidase (XOD) activity, and mRNA expression of hypoxanthine guanine-phosphoribosyltransferases transferase (HPRT1...
Source: Journal of Pharmacological and Toxicological Methods - February 21, 2020 Category: Drugs & Pharmacology Source Type: research

Systematic characterization of AT1 receptor antagonists with label-free dynamic mass redistribution assays
This study demonstrated that the DMR assay has great potential for determining the pharmacological parameters of ligands. This work may serve as guidance for other receptor and ligand assays. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - February 21, 2020 Category: Drugs & Pharmacology Source Type: research

Comparison of sublingual, facial and retro-bulbar blood sampling in mice in relation to animal welfare and blood quality
This study investigated 1) which of the three blood sampling techniques had the least impact on nest building activity, level of faecal corticosterone metabolites, body weight, fur status, and macroscopic changes, 2) whether the blood sampling techniques gave rise to variation in blood quality between blood samples, and 3) whether sublingual and facial vein puncture should be performed with or without anaesthesia in female C57BL/6 mice.MethodThree hundred and sixty C57BL/6 female mice divided into five batches were included in the study and randomized to a short (blood sampling on Day 8, 9 and 10) or a long protocol (blood...
Source: Journal of Pharmacological and Toxicological Methods - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

A pharmacological characterization of electrocardiogram PR and QRS intervals in conscious telemetered rats
DiscussionAt similar plasma concentrations to other species, the conscious telemetered rat demonstrates limited utility in assessing PR interval prolongation by CCBs, despite significant contractility effects being observed. However, results with SCBs demonstrate a potential application for evaluating drug-induced QRS prolongation. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - February 1, 2020 Category: Drugs & Pharmacology Source Type: research

Antibodies against polyethylene glycol in human blood: A literature review
Publication date: Available online 23 January 2020Source: Journal of Pharmacological and Toxicological MethodsAuthor(s): Lu Hong, Zongkui Wang, Xin Wei, Jianyou Shi, Changqing LiAbstractPolyethylene glycol (PEG) conjugation, i.e. PEGylation, is a successful strategy to improve the pharmacokinetics and pharmacodynamics of biopharmaceuticals. In the past few decades, PEGylation technology has developed tremendously, and>15 PEGylated therapeutics have been brought to market, with more in development. However, the widely accepted assumption that PEG would have no antigenicity or immunogenicity is increasingly challenged with p...
Source: Journal of Pharmacological and Toxicological Methods - January 23, 2020 Category: Drugs & Pharmacology Source Type: research

Regulatory safety pharmacology evaluation of BIA 10-2474
In conclusion, BIA 10–2474 had no adverse effects on any of the major vital organ systems, and, unfortunately, the data shed no further light on the mechanism(s) responsible for the clinical trial accident with BIA 10–2474. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - January 22, 2020 Category: Drugs & Pharmacology Source Type: research

Marble-burying behavior test as a murine model of compulsive-like behavior
In conclusion, there appears a need to address the issue of construct and predictive validity of the model authoritatively; or the paradigm may remain squandered in the field of obsessive-compulsive disorder research. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - January 18, 2020 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: January–February 2020Source: Journal of Pharmacological and Toxicological Methods, Volume 101Author(s): (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - January 9, 2020 Category: Drugs & Pharmacology Source Type: research

A porcine model of ureteral contractile activity: Influences of age, tissue orientation, region, urothelium, COX and NO
DiscussionThe complexity of ureteral contractions depicting bursts of phasic activity requires AUC assessment. Porcine ureteral contractile properties, such as regional differences, influence of mucosa and lack of response to carbachol, are similar to those reported in the literature for human ureter. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - December 25, 2019 Category: Drugs & Pharmacology Source Type: research

Utilization of a mouse/human chimeric model for long term metabolic testing of human skin
Publication date: Available online 16 December 2019Source: Journal of Pharmacological and Toxicological MethodsAuthor(s): P. Quantin, K. Ghazi, U. Pereira, A. Smith, L. Degardin, P. Vigneron, H. Ficheux, J. Garlick, L. Rapetti, C. EglesAbstractUntil now, ex vivo human skin explant utilization in tissue culture has consisted of limited short-term studies (less than a week). This short timeframe does not allow for the investigation of metabolic responses of complex tissues to specific molecules or compounds. Here, we aim to develop an improved mouse transplantation model that maintains the viability, structure and functional...
Source: Journal of Pharmacological and Toxicological Methods - December 18, 2019 Category: Drugs & Pharmacology Source Type: research

A low-cost, accurate method for detecting reticulocytes at different maturation stages based on changes in the mitochondrial membrane potential
Publication date: Available online 16 December 2019Source: Journal of Pharmacological and Toxicological MethodsAuthor(s): Ruilan Du, Haikang Bei, Lihong Jia, Chunyan Huang, Qizhu Chen, Jinquan Wang, Fenglin Wu, Jun Chen, Huaben BoAbstractIn the clinical setting, reticulocytes are used as an index for the hematopoietic function of the bone marrow. Different maturation stages of reticulocytes are early markers for bone marrow hematopoietic stem cell transplantation and bone marrow regeneration after chemotherapy. Therefore, we aimed to establish a method for detecting the different reticulocyte maturation stages. Based on th...
Source: Journal of Pharmacological and Toxicological Methods - December 18, 2019 Category: Drugs & Pharmacology Source Type: research

Generation of a robust model for inducing autoimmune arthritis in Sprague Dawley rats
DiscussionIn a head-to-head comparison between SD and Wistar rats, the disease profile was better sustained and consistent in SD rats, and the use of CII with low dose CFA and LPS induced features akin to human RA. Taken together, a reproducible model of arthritis was established which can be replicated in any laboratory with limited resources. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - December 18, 2019 Category: Drugs & Pharmacology Source Type: research

Three-dimensional integrated quantitative modeling and fluorescent imaging of doxorubicin-induced cardiotoxicity in a whole organ using a deconvolution microscope
Publication date: Available online 16 December 2019Source: Journal of Pharmacological and Toxicological MethodsAuthor(s): Bo Chen, Jing-Pu ZhangAbstractThe quantification and visualization of fluorescent staining at the whole organ level remain a challenge. Deconvolution image systems allow multi-dimensional imaging and stereo-measurement via rapid 3D reconstruction. To demonstrate this technique, we investigated doxorubicin-induced cardiotoxicity in zebrafish. Fluorogenic probe and immunofluorescence were employed to identify cardiac reactive oxygen species generation and myocardial apoptosis, respectively. We revealed th...
Source: Journal of Pharmacological and Toxicological Methods - December 18, 2019 Category: Drugs & Pharmacology Source Type: research

Radiolabelling and immunohistochemistry reveal platelet recruitment into lungs and platelet migration into airspaces following LPS inhalation in mice
ConclusionsIntranasal administration of LPS activates the innate immune response which includes a fall in peripheral blood platelet counts with subsequent platelet recruitment to the lung, spleen and liver, measured by immunohistochemistry and radiolabelling techniques. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - December 13, 2019 Category: Drugs & Pharmacology Source Type: research

Evaluation of different intraarticular injection volumes to assess optimum efficient amount; an experimental study in rat knee joints
In conclusion, the injection volume for the knee joints of rats should not exceed the 30 μL for optimum efficiency. (Source: Journal of Pharmacological and Toxicological Methods)
Source: Journal of Pharmacological and Toxicological Methods - November 29, 2019 Category: Drugs & Pharmacology Source Type: research