Chemometric Discrimination of Three Pistacia species via their Metabolic Profiling and Their Possible in Vitro Effects on Memory Functions
In this study, the metabolic profiling of Pistacia leaves extracts was achieved via UHPLC-ESI-MS analysis and GC-MS analysis employing chemometric analysis for their discrimination. In addition, the methanolic extracts of different Pistacia species were assessed for their anti-cholinesterase and anti-inflammatory activities by various in vitro assays. 37 and 30 metabolites belonging to different classes were identified by UHPLC-ESI-MS and GC-MS analyses respectively. Chemometric analysis revealed that P. lentiscus and P. khinjuk were more closely related chemically to each other. All studied Pistacia leaves extracts...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 29, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of Prenylflavonoids from Aerial Parts of Epimedium grandiflorum and Dietary Supplements using HPTLC, UHPLC-PDA and UHPLC-QToF along with Chemometric Tools to Differentiate Epimedium Species
Publication date: Available online 28 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ji-Yeong Bae, Bharathi Avula, Jianping Zhao, Vijayasankar Raman, Yan-Hong Wang, Mei Wang, Fazila Zulfiqar, Wei Feng, Jong Hee Park, Naohito Abe, Zulfiqar Ali, Ikhlas A. KhanABSTRACTAn UHPLC method was developed for the determination of 15 prenylflavonoids from aerial parts of Epimedium grandiflorum and related species (Berberidaceae). The separation was achieved using a reverse phased column and water/acetonitrile gradient as a mobile phase, and a temperature of 40°C. The developed analytical method was ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 29, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a Highly Efficient Decontamination Approach for Ceftolozane in the Pharmaceutical Manufacturing Environment
Publication date: Available online 29 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rong-Sheng Yang, Li-Kang Zhang, Roy Helmy, Teresa Andreani, Zhijian Liu, Huaming ShengAbstractThe β-lactam core is a key structure responsible for inducing both IgE-mediated acute-onset hypersensitivity and T-cell-mediated delayed-onset hypersensitivity with penicillins in humans. There is essentially no clinically significant immunologic cross-reactivity noted between the β-lactam cores of penicillins and cephalosporins based on challenge studies in humans. The side-chains appear to be more import...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 29, 2019 Category: Drugs & Pharmacology Source Type: research

Application of in-sample calibration curve methodology for regulated bioanalysis: critical considerations in method development, validation and sample analysis
Publication date: Available online 29 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Long Yuan, Huidong Gu, Jianing Zeng, Renuka Pillutla, Qin C. JiAbstractTraditionally, for a liquid chromatography tandem mass spectrometry (LC-MS/MS) bioanalytical assay, an external calibration curve is required to achieve accurate quantitation of an analyte. Recently, a novel in-sample calibration curves (ISCC) methodology that can achieve quick and accurate LC-MS/MS bioanalysis without the use of an external calibration curve was reported. The ISCC methodology utilizes the presence of multiple naturally o...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 29, 2019 Category: Drugs & Pharmacology Source Type: research

Selective Quantitation of Co-Formulated Ternary Mixture in the Presence of Potential Impurities by Liquid Chromatographic Methods
Publication date: Available online 27 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ahmed S. Fayed, Shereen A. Boltia, Awadh Musaed, Maha A. HegazyAbstractTwo high performance chromatographic methods were developed and validated for the simultaneous determination of Ambroxol, Guaifenesin and Theophylline in pharmaceutical dosage forms and in the presence of Guaiacol and Caffeine as the officially stated impurities. These were a reversed phase liquid and a thin layer chromatographic methods. The liquid chromatographic separation was achieved using Inertsil ODS-3 C18 column (4.6 mm&thi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Anti-infliximab antibodies: how to compare old and new data?
ConclusionADA concentrations measured with the different assays were compared and a cutoff concentration was determined for each of them to distinguish between low and high ADA concentrations. These cutoff concentrations may serve as a tool for clinicians to make treatment decisions for patients with a loss of response to infliximab and undetectable infliximab serum concentrations. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of a chiral UHPLC-MS method for the analysis of cysteine enantiomers in biological samples
Publication date: Available online 27 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lucia Pucciarini, Víctor González-Ruiz, Josephine Zangari, Jean-Claude Martinou, Benedetto Natalini, Roccaldo Sardella, Serge RudazAbstractFor years, d-amino acids were thought to have a minor function in biological processes compared to that of l-enantiomers. Recently, many studies have shown that d-amino acids are present in high concentrations in microorganisms, plants, mammals and humans and execute specific biological functions. One relevant example is that of d-cysteine, whose hydrogen su...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of raltegravir and raltegravir glucuronide in human plasma and urine by LC-MS/MS with application in a maternal-fetal pharmacokinetic study
Publication date: Available online 27 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fernanda de Lima Moreira, Maria Paula Marques, Geraldo Duarte, Vera Lucia LanchoteAbstractRaltegravir (RAL) is a HIV-integrase inhibitor recommended for treatment of HIV type 1 infection during pregnancy. The elimination of RAL to RAL glucuronide (RAL GLU) is mediated primarily by UDP glucuronosyltransferase 1A1 (UGT1A1). The present study shows the development and validation of 4 different methods for the analysis of RAL and RAL GLU in plasma and in urine samples. The methods were applied to evaluate the ma...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative determination of characteristic components from compound of Lysionotus pauciflorus Maxim. by LC-MS/MS and its application to a pharmacokinetic study
Publication date: Available online 27 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Caijuan Liang, Jintuo Yin, Yinling Ma, Xia zhang, Lantong ZhangAbstractTuberculosis of cervical lymph nodes is called scrofula in Traditional Chinese Medicine (TCM). Clinical manifestation is that unilateral or bilateral neck can have multiple enlarged lymph nodes of different sizes. Current therapeutic drugs include Lysionotus pauciflorus Maxim. tablets and compound of Lysionotus pauciflorus Maxim., which have a significant effect on tuberculosis of cervical lymph nodes. This compound is composed of three h...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 25 October 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 175Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Publisher's Note
Publication date: 25 October 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 175Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

An integrated metabolomics strategy to reveal dose-effect relationship and therapeutic mechanisms of different efficacy of rhubarb in constipation rats
Publication date: Available online 26 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jia-Qian Chen, Yan-Yan Chen, Hui-Juan Tao, Zong-Jin Pu, Xu-Qin Shi, Jing Zhang, Ya-Jie Tan, Shi-Jun Yue, Gui-Sheng Zhou, Er-Xin Shang, Yu-Ping Tang, Jin-Ao DuanAbstractThe ambiguity of dose-effect relationship of many traditional Chinese medicines (TCMs) has always influenced their rational use in TCM clinic. Rhubarb, a preferred representative of cathartic TCM, is currently widely used that results in a diversity of its dosage. The aim of this study was to use an integrated metabolomics strategy to simultan...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 27, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of multiple components in rat plasma and pharmacokinetic studies at a pharmacodynamic dose of Xian-Ling-Gu-Bao capsule by UPLC-MS/MS
Publication date: Available online 26 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xi-yang Tang, Zi-qin Dai, Qing-chang Wu, Jia-xing Zeng, Meng-xue Gao, Hui-hui Xiao, Zhi-hong Yao, Yi Dai, Xin-sheng YaoAbstractXian-Ling-Gu-Bao capsule (XLGB) is an effective traditional Chinese medicine prescription (TCMP) that is used for the prevention and treatment of osteoporosis in China. A rapid, simple, efficient and stable method based on UPLC-MS/MS technology was developed for simultaneous determination of multiple components of XLGB in rat plasma. Mass spectrometric detection was performed in mult...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 27, 2019 Category: Drugs & Pharmacology Source Type: research

Investigation of tryptophan-related yellowing in parenteral amino acid solution: development of a stability-indicating method and assessment of degradation products in pharmaceutical formulations
Publication date: Available online 27 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Nina Unger, Antonio Ferraro, Ulrike HolzgrabeAbstractParenteral amino acid solutions containing tryptophan tend to develop a yellow colouration upon storage. Hence, the aim of the present study was to find out whether tryptophan degradation products are the reason for the yellowing. The degree of discolouration and tryptophan degradation was examined by visual examination and UV/Vis measurements with respect to oxygen presence, pH value, and duration of steam sterilization. LC-UV analyses of autoclaved trypt...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 27, 2019 Category: Drugs & Pharmacology Source Type: research

An analytical strategy to explore the multicomponent pharmacokinetics of herbal medicine independently of standards: Application in Gelsemium elegans extracts
This study was the first attempt to systematically reveal the in vivo pharmacokinetics of Gelsemium elegans. This strategy and methodology will find widespread use in the quantitative pharmacokinetic analysis of multiple components independently of standards for herbal medicine, among other applications.Graphical Abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2019 Category: Drugs & Pharmacology Source Type: research

Toxicological screening and DNA sequencing detects contamination and adulteration in regulated herbal medicines and supplements for diet, weight loss and cardiovascular health
This study demonstrates that, despite current monitoring approaches, contaminated and adulterated products are still reaching the consumer. We suggest that a better solution is stronger pre-market evaluation, using techniques such as that outlined in this study. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2019 Category: Drugs & Pharmacology Source Type: research

Current application and potential use of GC × GC in the pharmaceutical and biomedical field
Publication date: 30 November 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 176Author(s): Juan Aspromonte, Kris Wolfs, Erwin AdamsAbstractThe analysis of volatile samples is mostly performed by GC. However, its separation power may not be enough for some complex samples. Comprehensive gas chromatography (GC × GC) increases the separation power by means of a second separation dimension. Parallel to the great technological advances realised since the early stages of the technique, considerable applications in different fields have emerged. Nonetheless, it is still often seen as a novelty, th...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2019 Category: Drugs & Pharmacology Source Type: research

Silane-based Self-Assembled Monolayer Deposited on Fluorine Doped Tin Oxide as Model System for Pharmaceutical and Biomedical Analysis
Publication date: Available online 22 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Inga Gabriunaite, Aušra Valiūnienė, Margarita Poderyte, Arunas RamanaviciusAbstractRecently, self-assembled monolayers (SAMs) are gaining a lot of interest due to their simplicity of preparation and wide applicability in the development of model systems used in pharmaceutical and biomedical analysis. The most efficient methods used for the investigation of SAM-based structures usually include cyclic voltammetry and electrochemical impedance spectroscopy. Scanning electrochemical microscopy (SECM) co...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2019 Category: Drugs & Pharmacology Source Type: research

Part 2: Physicochemical characterization of bevacizumab in 2 mg/mL antibody solutions as used in human i.v. administration: Comparison of originator with a biosimilar candidate
Publication date: 30 November 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 176Author(s): Tudor Arvinte, Caroline Palais, Emilie Poirier, Amelia Cudd, Shanthi Rajendran, Stephen Brokx, Jason DowdAbstractThe physicochemical properties of Avastin® manufactured in the USA (Originator USA) and in Europe (Originator EU) and ABX-BEV, a bevacizumab biosimilar drug product candidate produced by Apobiologix Inc., were characterized at a clinically relevant concentration of 2 mg/mL following dilution of the 25 mg/mL drug products with 0.9% NaCl. Measurements using 14 orthogonal analytical methods perf...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 24, 2019 Category: Drugs & Pharmacology Source Type: research

Chromatographic Editing Enhances Natural Product Discovery
Publication date: Available online 21 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Matthew A. Knestrick, Rahmy Tawfik, Lindsey N. Shaw, Bill J. BakerAbstractFungi are known for their diverse biologically active secondary metabolites, compounds that have provided the basis for many landmark therapeutics in the last century. Due to ease of collection and culturing, the existing fungal chemical literature is vast, and fungal natural product isolation can often be hindered by the numerous nuisance and pan-toxic compounds that many strains produce. Dereplication efforts, aimed at identifying su...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 23, 2019 Category: Drugs & Pharmacology Source Type: research

Targeted amino acid and related amines analysis based on iTRAQ®-LC-MS/MS for discovering potential hepatotoxicity biomarkers
Publication date: Available online 19 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhuoling An, Ting Hu, Yali Lv, Pengfei Li, Lihong LiuAbstractDrug induced liver injury (DILI) is a diverse set of liver injury occurring after exposure to any manufactured or naturally occurring chemical compounds. DILI has already become the leading cause of acute liver failure in developed countries. Early diagnosis is of great significance for the prevention and treatment of DILI, which can effectively avoid its progress to acute liver failure. In this paper, a novel targeted metabolomics method based on ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 20, 2019 Category: Drugs & Pharmacology Source Type: research

Chromatographic studies of unusual on-column degradation of cefaclor observed in the impurity separation by HPLC
Publication date: Available online 19 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jia-Sheng Zhong, Yan-Huang, Jin-Zhi Wan, Xiao-Yang Yu, Ai-Ling Yu, Huan-Xiang Zeng, Zhen-Yang Chen, Xiang-Rong Zhou, Mei-DingAbstractAn unpredictable ghost peak was intermittently observed during the impurity separation of cefaclor and formulation by high performance liquid chromatography (HPLC) with contents below the reported threshold of approximately 0.3% in different laboratories. Through a series of investigations, the ghost peak was identified as an unusual on-column degradant of cefaclor formed under...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 20, 2019 Category: Drugs & Pharmacology Source Type: research

Mixed monolayer decorated SPR sensing surface for thrombin detection
Publication date: Available online 19 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Demet Ataman Sadık, İsmail Hakkı Boyacı, Mehmet MutluAbstractThe development of surface plasmon resonance (SPR) based immunosensor for thrombin detection was aimed. For this purpose, 3,3’ Dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP):6-mercapto-1-hexanol (MCH) mixed self-assembled monolayers (mSAMs) were formed on gold surfaces for immobilization of anti-thrombin antibody. The performance of the immunosensor was determined against the target protein thrombin at various concentrations...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 20, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid Characterization of Compounds in Fupo Ganmao Granules by High-performance Liquid Chromatography Tandem Mass Spectrometry
In this study, the components of FP were profiled and characterized using HPLC-ESI-MS2. A total of 58 compounds were identified according to their fragmentation features in IT-TOF-MS and IT-MS2, including 13 phenolic acids, 17 flavonoids, 21 lignans, 2 alkaloids, and 5 other compounds. Among them, the identities of 8 compounds were explicitly confirmed by comparing them with standard compounds, and their contents were determined. Our study provided a comprehensive understanding of the diverse chemical profile in FP, and the approach we developed is useful for the analysis of other TCM formulas. (Source: Journal of Pharmace...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 19, 2019 Category: Drugs & Pharmacology Source Type: research

Current application and potential use of GC×GC in the pharmaceutical and biomedical field
Publication date: Available online 18 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Juan Aspromonte, Kris Wolfs, Erwin AdamsAbstractThe analysis of volatile samples is mostly performed by GC. However, its separation power may not be enough for some complex samples. Comprehensive gas chromatography (GC × GC) increases the separation power by means of a second separation dimension. Parallel to the great technological advances realised since the early stages of the technique, considerable applications in different fields have emerged. Nonetheless, it is still often seen as ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 19, 2019 Category: Drugs & Pharmacology Source Type: research

Blood-Brain Barrier Permeability Study of Ginger Constituents
Publication date: Available online 19 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alexandra Simon, András Darcsi, Ágnes Kéry, Eszter RiethmüllerABSTRACTGinger, the rhizome of Zingiber officinale Roscoe is of great importance in the traditional medicine for the treatment of various diseases. More than 400 constituents have been reported in the plant, the most important ones being the gingerol and shogaol derivatives. Positive effects of ginger extracts and isolated [6]-gingerol have been proved in animal models of anxiety, Alzheimer’s disease, Parkinson&rsqu...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 19, 2019 Category: Drugs & Pharmacology Source Type: research

A rapid and specific method to simultaneously quantify eukaryotic Elongation Factor 1A1 and A2 protein levels in cancer cells
ConclusionsICW is a valuable tool to specifically determine the intracellular level of eEF1A1-A2 proteins thus contributing to better define their role as potential therapeutic targets and prognostic markers in human tumors as well as for drug effects screening. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 18, 2019 Category: Drugs & Pharmacology Source Type: research

Fast liquid chromatography-tandem mass spectrometry method for simultaneous determination of eight antiepileptic drugs and an active metabolite in human plasma using polarity switching and timed selected reaction monitoring
Publication date: Available online 17 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maureen A. KaneAbstractA fast liquid chromatography-tandem mass spectrometry method using polarity switching and timed selected reaction monitoring has been developed and validated to quantify eight antiepileptic drugs and an active drug metabolite in human plasma within a single analytical assay. The antiepileptic drugs include levetiracetam, lamotrigine, zonisamide, topiramate, carbamazepine, phenytoin, divalproex sodium, oxcarbazepine, and the oxcarbazepine active metabolite 10,11-dihydro-10-hydroxycarbam...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 18, 2019 Category: Drugs & Pharmacology Source Type: research

Development and Validation of an LC-MS/MS Method for the Simultaneous Determination of Bedaquiline and Rifabutin in Human Plasma
This article describes the simultaneous determination of bedaquiline fumarate (TMC-207) and rifabutin in human plasma by stable isotope dilution tandem mass spectrometry. The methodology was developed for an investigation of potential drug-drug interactions of the two anti-tuberculosis drugs when given together to healthy human volunteers. Use of the two drugs in combination to treat disease caused by Mycobacterium tuberculosis is contemplated as the bacterium becomes resistant to many currently available drugs. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 18, 2019 Category: Drugs & Pharmacology Source Type: research

A Sensitive and Efficient Method for Simultaneous Profiling of Bile Acids and Fatty Acids by UPLC-MS/MS
Publication date: Available online 15 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ting Hu, Zhuoling An, Chen Shi, Pengfei Li, Lihong LiuAbstractThe carboxyl group is the functional group in both bile acids (BAs) and fatty acids (FAs) (BAFAs). Considering the functional correlation and the structural similarity of these compounds, a sensitive and efficient method was developed here for the first time to simultaneously profile BAFAs based on ultrahigh performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). Through optimization of the chromatographic conditions,...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 16, 2019 Category: Drugs & Pharmacology Source Type: research

A novel neutral loss/product ion scan-incorporated integral approach for the untargeted characterization and comparison of the carboxyl-free ginsenosides from Panax ginseng, Panax quinquefolius, and Panax notoginseng
Publication date: Available online 14 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Wen-zhi Yang, Xiao-jian Shi, Chang-liang Yao, Yong Huang, Jin-jun Hou, Su-mei Han, Zi-jin Feng, Wen-long Wei, Wan-ying Wu, De-an GuoAbstractDifferentiated composition in precursor ions for different subclasses of ginsenosides in the negative electrospray-ionization mode has been reported, which lays a foundation for the sorted and untargeted identification of ginsenosides. Carboxyl-free ginsenosides simultaneously from Panax ginseng, P. quinquefolius, and P. notoginseng, were comprehensively characterized an...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 15, 2019 Category: Drugs & Pharmacology Source Type: research

Development of an LC-MS/MS method for quantitative analysis of Chlorogenic acid in human plasma and its application to a pharmacokinetic study in Chinese patients with advanced solid tumor
Publication date: Available online 14 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fen Yang, Lin Shen, Chen Zhang, Fu rong Kou, Jing Gao, Yan Li, Guo bing Xu, Ji fang GongAbstractA simple and specific, rapid resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of chlorogenic acid in human plasma using neochlorogenic acid as the internal standard. Plasma samples were precipitated with methanol and separated on a Zorbax C18 column (50 × 2.1 mm, i.d. 1.8 µm) at a flow rate of 0.4&thins...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 15, 2019 Category: Drugs & Pharmacology Source Type: research

Screening of cathepsin B inhibitors in traditional Chinese medicine by capillary electrophoresis with immobilized enzyme microreactor
Publication date: Available online 13 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Baofang Zhang, Zilin ChenAbstractA simple and valid method for rapid screening of cathepsin B inhibitors from traditional Chinese medicines (TCMs) was established by the combination of immobilized enzyme microreactor (IMER) and capillary electrophoresis. Cathepsin B was immobilized on the inner surface of the capillary by glutaraldehyde method. The separation of substrate and product could be finished by baseline within 3 min. The activity of the immobilized cathepsin B remained approximately 90% afte...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 14, 2019 Category: Drugs & Pharmacology Source Type: research

Investigating oral fluid and exhaled breath as alternative matrices for anti-doping testing: analysis of 521 matched samples
Publication date: Available online 12 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Geoffrey D. Miller, Ryan M. Van Wagoner, Benjamin B. Bruno, Jacob Husk, Matthew N. Fedoruk, Daniel EichnerAbstractCurrent anti-doping testing is primarily conducted in urine and blood. Recently, due to confounding factors with urine and blood collections such as invasiveness, cost, and stringent shipping conditions, there has been a push for the use of alternative sample matrices to ameliorate these issues. Gaining support within the anti-doping field is the use of oral fluid, and more recently exhaled breat...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 14, 2019 Category: Drugs & Pharmacology Source Type: research

Application of the optimized and validated LC–MS method for simultaneous quantification of tryptophan metabolites in culture medium from cancer cells
Publication date: 30 November 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 176Author(s): Ilona Sadok, Kamila Rachwał, Magdalena StaniszewskaAbstractKynurenine pathway is the main route of tryptophan degradation generating a number of immunoregulatory compounds. Some conditions like oxidative stress, inflammatory factors might enhance tryptophan degradation. Process is active in several cells including fibroblasts, cancer cells, and immune cells, therefore it is intensively studied in context of cancer microenvironment. The validated and standardized methodology for kynurenine quantification is cru...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 14, 2019 Category: Drugs & Pharmacology Source Type: research

Enantioselective separation of eight antihistamines with α1-acid glycoprotein-based chiral stationary phase by HPLC: Development and validation for the enantiomeric quality control
Publication date: Available online 10 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bolin Zhu, Mengyao Xue, Beibei Liu, Qing Li, Xingjie GuoAbstractIn the present paper, a sensitive and selective chiral LC method has been developed for enantiomeric separation and determination of eight antihistamines in pharmaceutical formulations. Enantiomeric separation was achieved on the α1-acid glycoprotein-based chiral stationary phase known as Chiralpak AGP. The effects of the mobile phase composition, type and proportion of organic modifier and the concentration and pH of the buffer on retenti...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2019 Category: Drugs & Pharmacology Source Type: research

Part 2: Physicochemical characterization of bevacizumab in 2 mg/mL antibody solutions as used in human i.v. administration: comparison of originator with a biosimilar candidate
Publication date: Available online 10 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tudor Arvinte, Caroline Palais, Emilie Poirier, Amelia Cudd, Shanthi Rajendran, Stephen Brokx, Jason DowdAbstractThe physicochemical properties of Avastin® manufactured in the USA (Originator USA) and in Europe (Originator EU) and ABX-BEV, a bevacizumab biosimilar drug product candidate produced by Apobiologix Inc., were characterized at a clinically relevant concentration of 2 mg/mL following dilution of the 25 mg/mL drug products with 0.9% NaCl. Measurements using 14 orthogonal analytical...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2019 Category: Drugs & Pharmacology Source Type: research

Separation and characterization of forced degradation products in homoharringtonine injection by UHPLC-Q-TOF-MS
Publication date: Available online 10 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiao Gu, Yue Chen, Yongjun Lou, Jinqi ZhengAbstractA rapid ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was developed for separation and characterization of the degradation products in HHT injection. Chromatographic separation was achieved on a ZORBAX Eclipse XDB-C18 HD column (2.1 × 100 mm, 1.8μm) using methanol- ammonium formate (pH 3.0; 30 mM) (30:70, v/v) as mobile phase in an isocratic mode of elution. Forced degrad...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2019 Category: Drugs & Pharmacology Source Type: research

Subminute micro-isolation of pharmaceuticals with ultra-high pressure liquid chromatography
In this study we describe the isolation of small molecule and biomolecule crude mixtures at the microgram-scale (100 – 2500 µg) in single or library format with methods as fast as 1.0 min and system pressures averaging 10000 psi with an ultra-high pressure liquid chromatography (UHPLC) setup. UHPLC technology provides several advantages for rapid (
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2019 Category: Drugs & Pharmacology Source Type: research

LC-MS/MS combined with in vivo microdialysis sampling from conscious rat striatum for simultaneous determination of active constituents of Yuanhu- Baizhi herb pair and endogenous neurotransmitters: application to pharmacokinetic and pharmacodynamic study
In conclusion, the pharmacokinetic and pharmacodynamic study based on in vivo microdialysis sampling from awaken animals and LC-MS/MS quantitation is in favor of revealing the compatibility mechanism of herb pairs. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2019 Category: Drugs & Pharmacology Source Type: research

Chemical profliling of Dingkun Dan by Ultra High Performance Liquid Chromatography Q Exactive Orbitrap High Resolution Mass Spectrometry
Publication date: Available online 6 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaoxia Gao, Nan Wang, Jinping Jia, Peiyi Wang, Airong Zhang, Xuemei QinAbstractDingkun Dan (DKD) has been widely used for a variety of gynecological disease. However, the systematic analysis of the chemical constituents of DKD has not been well established because of the complexity of the formula and confidentiality. In this paper, liquid chromatography Q Exactive high resolution accurate mass spectrometry (UHPLC-QE-HRMS) with automated MetaboLynx analysis was established to characterize the chemical constitue...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Fabrication of carbon nanotube-polylactic acid composite electrode by melt compounding for capillary electrophoretic determination of tectoridin and irigenin in Belamcandae Rhizoma
Publication date: Available online 10 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hua Mao, Xinming Ye, Weilong Chen, Wenye Geng, Gang ChenAbstractA sensitive detection electrode based on the composite of carbon nanotubes (CNTs) and polylactic acid (PLA) was fabricated for measuring the bioactive constituents in Belamcandae Rhizoma by capillary electrophoresis (CE). The composite was facilely fabricated by packing a blend of CNTs and melted PLA into a fused silica capillary under heat. The prepared CNT-PLA composite was characterized by scanning electron microscopy, Raman spectroscopy, Fouri...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Optimization of an in vitro gut microbiome biotransformation platform with chlorogenic acid as model compound: from fecal sample to biotransformation product identification
This study optimized a ready-to-use platform to investigate colonic biotransformation of xenobiotics by using chlorogenic acid as a model compound. This platform can be used in the future to study differences in colonic biotransformation of xenobiotics using fecal samples of different patient groups.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Novel “Matrix-Corrected Calibration” study for the detection of polyunsaturated fatty acids (PUFAs) in plasma and erythrocytes by means of a gas chromatography-mass spectrometry approach optimized to follow up long-term parental patients
Publication date: Available online 24 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Eugenio Alladio, Alessandra MancaGraphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Electrochemical behaviour of anticancer drug lomustine and in situ evaluation of its interaction with DNA
Publication date: Available online 25 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Paulina Andréa V. de Carvalho, Ilanna Campelo Lopes, Elizaura Hyeda C. Silva, Evellin Enny S. Bruzaca, Hugo José Chaves, Mayara Ingrid S. Lima, Auro Atsushi TanakaAbstractElectrochemical techniques were used to investigate the behavior of lomustine (CCNU) and its degradation in aqueous solution at a glassy carbon electrode (GCE). The in situ interaction of CCNU and chemically degraded CCNU (cdCCNU) with dsDNA was then investigated in dsDNA incubated solutions, using dsDNA electrochemical biosenso...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Human Gastrointestinal Metabolism of the Anti-rheumatic Fraction of Dianbaizhu (Gaultheria leucocarpa var. yunnanensis) in Vitro: Elucidation of the Metabolic Analysis in Gastric Juice, Intestinal Juice and Human Intestinal Bacteria by UPLC-LTQ-Orbitrap-MSn and HPLC-DAD
Publication date: Available online 26 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiuhuan Wang, Xiao Li, Rufeng Wang, Le Wang, Shusheng Fan, Xiaoping Wang, Xiao Xu, Xin Yan, Ting He, Xueyang Ren, Gaimei SheAbstractThe anti-rheumatic fraction (ARF), is responsible for the anti-inflammatory and analgesic effects of Dianbaizhu derived from the aerial part of Gaultheria leucocarpa var. yunnanensis (Ericaceae). The gastrointestinal metabolism of ARF was investigated in vitro through simulating a series of models–gastric juice, intestinal juice, and human intestinal bacteria, analyzed by HP...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Acetone immersion enhanced MALDI-MS imaging of small molecule metabolites in biological tissues
Publication date: Available online 1 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chenglong Sun, Zhichao Li, Chunxia Ma, Qingce Zang, Jiangshuo Li, Wei Liu, Hengqiang Zhao, Xiao WangABSTRACTProfiling the endogenous tissue metabolites with spatial features is significant for our understanding of molecular histology, and provides an insightful way to uncover the complex associations between tissue metabolic response and external stimuli. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is an effective molecular imaging technology to illustrate the spatial loc...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Multi-platform metabonomics unravel amino acids as markers of HIV/combination antiretroviral therapy-induced oxidative stress
Publication date: Available online 2 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lungile J. Sitole, Fidele Tugizimana, Debra MeyerAbstractInfection by the human immunodeficiency virus (HIV) elicits an immune response wherein neutrophils produce reactive oxygen species (ROS) to defend against pathogen invasion. Consequently, disproportionate levels of ROS in relation to antioxidants lead to oxidative stress (OS), which plays a key role in HIV disease progression and pathogenesis. There is a close relationship between oxidative stress status and HIV infection/progression, both separately an...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Effective Extraction with Deep Eutectic Solvents and Enrichment by Macroporous Adsorption Resin of Flavonoids from Carthamus tinctorius L.
In conclusion, the developed DES2-UAE combined with macroporous resin enrichment can be an alternative method for the green and efficient extraction of bioactive flavonoids from plant materials. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

Application of the optimized and validated LC-MS method for simultaneous quantification of tryptophan metabolites in culture medium from cancer cells
Publication date: Available online 6 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ilona Sadok, Kamila Rachwał, Magdalena StaniszewskaAbstractKynurenine pathway is the main route of tryptophan degradation generating a number of immunoregulatory compounds. Some conditions like oxidative stress, inflammatory factors might enhance tryptophan degradation. Process is active in several cells including fibroblasts, cancer cells, and immune cells, therefore it is intensively studied in context of cancer microenvironment. The validated and standardized methodology for kynurenine quantification is c...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 6, 2019 Category: Drugs & Pharmacology Source Type: research