Development and validation of a UPLC-MS/MS method for simultaneous determination of vicagrel and its major metabolites in rat or human plasma: An optimized novel strategy for the stabilization of vicagrel
In this study, an optimized strategy was developed and validated to stabilize vicagrel, 2-oxo-clopidogrel (thiolactone metabolite), and H4 (active thiol metabolite) before quantification of the analytes, such as addition of citric acid (for plasma acidification) and NaF (a non-specific esterase inhibitor) to inhibit esterase activity, immediate addition of a thiol-alkylating reagent MPB into blood samples to derivatize H4 for the formation of stable H4 derivative (i.e., MP-H4), use of the anticoagulant K2EDTA to minimize the conversion of 2-oxo-clopidogrel to H-endo, and keeping the analytes at 4 °C or on wet ic...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 2, 2019 Category: Drugs & Pharmacology Source Type: research

A novel and sensitive HILIC-CAD method for glucosamine quantification in plasma and its application to a human pharmacokinetic study
Publication date: Available online 31 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chhavi Asthana, Gregory M. Peterson, Madhur D. Shastri, Rahul P. PatelAbstractThe clinical effect of glucosamine, the most widely used supplement in patients with osteoarthritis, on joint pain and function improvement, is reported to be inconsistent. Inter-patient variability in the pharmacokinetics of glucosamine, especially its oral absorption, could contribute to the inconsistent clinical outcomes. To test this hypothesis, a novel but simple Hydrophilic Interaction Liquid Chromatography coupled with Char...
Source: Journal of Pharmaceutical and Biomedical Analysis - November 2, 2019 Category: Drugs & Pharmacology Source Type: research

Biomedical analysis of New Psychoactive Substances (NPS) of natural origin
Publication date: Available online 30 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alfredo Fabrizio Lo Faro, Annagiulia Di Trana, Nunzia La Maida, Adriano Tagliabracci, Raffaele Giorgetti, Francesco Paolo BusardòAbstractNew psychoactive substances (NPS) can be divided into two main groups: synthetic molecules and active principles of natural origin. With respect to this latter group, a wide range of alkaloids contained in plants, mainly from Asia and South America, can be included in the class of NPS of natural origin. The majority NPS of natural origin presents stimulant and/or ha...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

Post-column infused internal standard assisted lipidomics profiling strategy and its application on phosphatidylcholine research
Publication date: Available online 30 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hsiao-Wei Liao, Ching-Hua Kuo, Hsi-Chun Chao, Guan-Yuan ChenAbstractVarious lipidomics studies have revealed the potential of using phospholipids as disease biomarkers for conditions such as Alzheimer’s disease, cancer, and sepsis. Establishing accurate quantification methods for targeted phospholipid analysis is important for making these potential markers more clinically relevant. Although a stable isotope labelled-internal standard method can provide good quantification accuracy for endogenous meta...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

The occurrence and exposure risk assessment of psychoactive drug residues and metabolites in aquatic environment
Publication date: Available online 31 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): D.P. Masemola, E.N. Nxumalo, H. Nyoni, TTI Nkambule, B.B Mamba, T.A.M. MsagatiAbstractIncreased use of psychoactive drugs and their continuous release into the aquatic environment threatens the aquatic life, human and animal’s life. As such this study was aimed at investigating the occurrence and the associated exposure human health risks of psychoactive drugs residues. The psychoactive drugs investigated include amphetamine, methylphenidate, heroin, cocaine and metabolites included are mainly morphin...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

An electrochemical signal-on apta-cyto-sensor for quantitation of circulating human MDA-MB-231 breast cancer cells by transduction of electro-deposited non-spherical nanoparticles of gold
Publication date: Available online 28 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): S. Akhtartavan, M. Karimi, N. Sattarahmady, H. HeliAbstractA highly simple, sensitive, specific and low-cost electrochemical apta-cyto-sensor for determination of circulating human MDA-MB-231 breast cancer cells was fabricated. Non-spherical nanoparticles of gold were electro-deposited in the presence of ethosuximide as a shape directing and size controlling agent. The nanoparticles had dimensions ranging 50-150 nm, and covered the underlying surface with a roughness factor of 8.03. The Non-spherical...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 30, 2019 Category: Drugs & Pharmacology Source Type: research

Detection and comparison of phenolic compounds in different extracts of black currant leaves by Liquid Chromatography coupled with High-Resolution ESI-LTQ-Orbitrap MS and High-Sensitivity ESI-Qtrap MS
Publication date: Available online 28 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gilda D’Urso, Paola Montoro, Sonia PiacenteAbstractLiquid preparations such as tinctures and infusion derived from Ribes nigrum L. (black currant) leaves are widely used in Europe. Despite the various uses reported for the infusion and hydroalcoholic extracts of the leaves of this species, their chemical profile has not yet been defined. In the present study, the infusion, hydroalcholic and methanolic extracts prepared from R. nigrum leaves were analyzed. LC-MS analyses of these extracts allowed the c...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 30, 2019 Category: Drugs & Pharmacology Source Type: research

A New High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for the Determination of Sunitinib and N-desethyl sunitinib in Human Plasma: Light-Induced Isomerism Overtaking Towards Therapeutic Drug Monitoring in Clinical Routine
Publication date: Available online 25 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Elena Marangon, Mauro Buzzo, Bianca Posocco, Sara Gagno, Martina Zanchetta, Valentina Iacuzzi, Ariana Soledad Poetto, Michela Guardascione, Luciana Giodini, Giuseppe ToffoliAbstractSunitinib is approved for advanced renal cell cancer, imatinib-resistant or -intolerant gastrointestinal stromal tumors and pancreatic neuroendocrine cancers. It is prescribed at a fixed dose but its plasma exposure shows large inter-individual variations. Taking into account the narrow therapeutic window and the positive exposur...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of total and free voriconazole in human plasma: application to pharmacokinetic study and therapeutic monitoring
Publication date: Available online 25 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Matylda Resztak, Katarzyna Kosicka, Paulina Zalewska, Justyna Krawiec, Franciszek K. GłówkaAbstractVoriconazole (VCZ) is highly efficient in the treatment of invasive fungal infections. However, the therapy requires the monitoring of VCZ plasma concentration, due to a narrow therapeutic index and large inter-individual variability of pharmacokinetics of VCZ, which often exhibits nonlinearity. Total plasma concentration of VCZ does not always provide sufficient information, especially in hemato-oncol...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Exclusion of unsuitable CNS drug candidates based on their physicochemical properties and unbound fractions in biomatrices for brain microdialysis investigations
Publication date: Available online 25 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Qianwen Wang, Tianjing Ren, Jiajia Zhao, Chun-Ho Wong, H.Y. Edwin Chan, Zhong ZuoAbstractMicrodialysis has been the only direct method of continuously measuring the unbound drug concentrations in extracellular fluid at a specific brain region with respect to time in the same animal. However, not every compound is suitable for microdialysis system as demonstrated by their inconsistent “by gain” and “by loss” in-vitro microdialysis probe recoveries leading to over- or under- estimated ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative, real-time in vivo tracking of magnetic nanoparticles using multispectral optoacoustic tomography (MSOT) imaging
Publication date: Available online 25 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tareq Anani, Andrew Brannen, Peter Panizzi, Evert C. Duin, Allan E. DavidAbstractThe goal of this work was to demonstrate real-time tracking of in vivo nanoparticle concentrations utilizing multispectral optoacoustic tomography (MSOT). Combining the high contrast of optical imaging with the high resolution of ultrasound imaging, MSOT was utilized for non-invasive, real-time tomographic imaging of particles in mice and the results calibrated against analysis of tissue samples with electron paramagnetic reson...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 5 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 177Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous Quantitative LC-MS Method of Ketamine, Midazolam and their Metabolites (Dehydronorketamine, Norketamine and 1Hydroxymidazolam) for its Application in Patients on Extracorporeal Membrane Oxygenation (ECMO) Therapy
Publication date: Available online 24 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ankit Rochani, Edwin Lam, Julian Tanjuakio, Hitoshi Hirose, Walter K. Kraft, Gagan KaushalAbstractKetamine (Ket) and midazolam (MDZ) are commonly administered drugs in the intensive care setting for analgesia and sedation. Ket and MDZ are metabolized to dehydro-norketamine (DHNK), nor-ketamine (NK) and 1-hydroxy midazolam (1HMDZ). Limited studies evaluating their pharmacokinetics exists in patients on extracorporeal membrane oxygenation (ECMO) therapy. Therefore, we developed a quantitative, high-performanc...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

UHPLC-MS/MS method for the quantification of aloin-A in rat plasma and its application to a pharmacokinetic study
Publication date: Available online 25 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chao Niu, Weijian Ye, Xiao Cui, Jia Sun, Shuyi Xiao, Gen Chen, Shihui Bao, Ruijie ChenAbstractAloin-A (also known as barbaloin), the main bioactive anthraquinone-C-glycoside of Aloe species, exhibits various beneficial pharmacological effects. However, the determination and pharmacokinetic study of aloin-A in rat plasma need to be improved and systematically demonstrated. In the present study, a simple, robust and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Labeled substance P as a neuropeptide reporter substance for enzyme activity
Publication date: Available online 24 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ulrich Schreiber, Christian Engl, Malte Bayer, Simone KönigAbstractRecently, we developed a bradykinin reporter assay and demonstrated the differing protease activity in Complex Regional Pain Syndrome patients vs. controls. In order to further characterize CRPS pathophysiology, the neuropeptide substance P was evaluated as possible reporter substance, here. It was labeled with a chromophore at the lysine residue and generated two major fragments following incubation with serum (amino acid residues 3-8 ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 24, 2019 Category: Drugs & Pharmacology Source Type: research

Optimization of a liquid chromatography method for the analysis of related substances in daclatasvir tablets using design of experiments integrated with the steepest ascent method and Monte Carlo simulation
Publication date: Available online 22 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Trupti Tol, Harshad Tawde, Savita Gorad, Avinash Jagdale, Amol Kulkarni, Avinash Kasbale, Anita Desai, Gautam SamantaAbstractAnalytical method for the determination of related substances (RS) in Daclatasvir tablets was optimised using quality by design (QbD) approach. Seven degradants (each more than 1.0%) generated during oxidation study, adversely affected the selectivity of the method. Coelution of the degradant peaks with API and known impurities, suggested failure in developing a stability indicating m...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 24, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic and Metabolic Profiling Studies of sennoside B by UPLC-MS/MS and UPLC-Q-TOF-MS
This study aimed to obtain the metabolic and pharmacokinetic data of sennoside B. The metabolic profiles of sennoside B were obtained from rat plasma, urine, bile and feces by an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). As a result, 14 metabolites were structurally identified and the proposed metabolic pathways of sennoside B included hydrolysis to aglycones, release of rhein-type anthrone, and extensive conjugation. As the only compound detected in the plasma samples after intravenous and intragastric administrations, the prototype was selected as the plasma mark...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 23, 2019 Category: Drugs & Pharmacology Source Type: research

Permeability and transport mechanism of trihexyphenidyl hydrochloride in Caco-2 cell monolayer model with a validated UPLC-MS/MS method
Publication date: Available online 21 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chengcan Lu, Kejia Fu, Kangna Cao, Jiali Wei, Jing Zhou, Di Zhao, Ning Li, Yang Lu, Xijing Chen, Yongjie ZhangAbstractA rapid and sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was applied to investigate the permeability characteristics and transport mechanism of trihexyphenidyl hydrochloride (TH) in D-hanks with the Caco-2 cells model. Analytes were separated using an Zorbax Extend-Agilent C18 (1.8 µm, 4.6 × 30 mm)...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 23, 2019 Category: Drugs & Pharmacology Source Type: research

Benchtop NMR spectroscopy in the analysis of substandard and falsified medicines as well as illegal drugs
Publication date: Available online 21 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Peter H.J. Keizers, Frank Bakker, José Ferreira, Paul F.K. Wackers, Dion van Kollenburg, Eva van der Aa, Amy van BeersAbstractSubstandard and falsified medical products may cause harm to patients and fail to treat the diseases or conditions for which they were intended. It is therefore required to have analytical methods available to assess medical product quality. Benchtop NMR spectroscopy provides a generic, inherently quantitative, analytical method capable of separating specific signals from thos...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 23, 2019 Category: Drugs & Pharmacology Source Type: research

HPLC–MS/MS method for the determination and pharmacokinetic study of six compounds against rheumatoid arthritis in rat plasma after oral administration of the extract of Caulophyllum robustum Maxim
Publication date: Available online 21 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yuyan Guo, Shaowa lü, Bingyou Yang, Guoyu Li, Wenbao Ma, Qiaoxin Guo, Qiuhong Wang, Haixue KuangAbstractCaulophyllum robustum Maxim (CRM) is a well-known traditional Chinese medicine (TCM) mainly present in the northeast, northwest and southwest regions of China, which is belong to the family Berberidaceae. The roots and rhizomes of CRM have been used as a famous TCM for the treatment of rheumatoid arthritis (RA). The selective, sensitive and accurate high-performance liquid chromatography-electrospray...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 21, 2019 Category: Drugs & Pharmacology Source Type: research

Effect of light, oxygen and temperature on the stability of artepillin C and p-coumaric acid from Brazilian green propolis
Publication date: Available online 19 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Caroline Arruda, Victor Pena Ribeiro, Marília Oliveira Almeida, Jennyfer Andrea Aldana Mejía, Rosana Casoti, Jairo Kenupp BastosAbstractBrazilian Green Propolis (BGP) is an important bee product, which displays important biological activities, making it valuable in the international market. The major prenylated phenolic compound in BPG is (E)-artepillin C, along with its precursor (E)-p-coumaric acid, both contributing to the biological effects of BGP. Taking that into account, it was evaluate...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 21, 2019 Category: Drugs & Pharmacology Source Type: research

Paper-based immunosensor with competitive assay for cortisol detection
Publication date: Available online 19 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Amara Apilux, Sirirat Rengpipat, Wilasinee Suwanjang, Orawon ChilapakulAbstractThe measurement of cortisol (stress hormone) is important for diagnosis and monitoring of stress-related diseases. A paper-based immunosensor with a competitive assay was developed for quick and easy detection of cortisol levels in serum. The paper-based sensor was fabricated using a simple and cheap wax printing method. Cortisol conjugated-BSA was immobilized on the paper’s surface in the detection zone for the competitive...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 21, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacopeial specifications and analytical data from post-marketing quality sampling and testing programs: a perspective beyond out-of-specification results
Publication date: Available online 18 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Edvaldo Oliveira Neves, Paloma Michelle de Sales, Damaris SilveiraAbstractEnsuring that marketed medicines meet acceptable standards (safety, quality, and efficacy) involves aspects of product development, compliance with good manufacturing practices, and monitoring and testing of these products already on the market. Pharmacopeias are one of the main tools used by regulatory authorities in the analytical testing for quality assessment; there are almost 60 pharmacopeias in the world. Thus, this research eva...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 20, 2019 Category: Drugs & Pharmacology Source Type: research

Solid-state analysis of amorphous solid dispersions: why dsc and xrpd may not be regarded as stand-alone techniques
Publication date: Available online 18 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sien Dedroog, Timothy Pas, Bjorn Vergauwen, Christophe Huygens, Guy Van den MooterAbstractAmorphous solid dispersions (ASDs) are single-phase amorphous systems, where drug molecules are molecularly dispersed (dissolved) in a polymer matrix. The molecular dispersion of the drug molecules is responsible for their improved dissolution properties. Unambiguously establishing the phase behaviour of the ASDs is of utmost importance. In this paper, we focused on the complementary nature of (modulated) differential ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 20, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative Study on Metabolic Profiling and Excretion in Rat Bile Between Combination of Notoginseng Total Saponins and Safflower Total Flavonoids and Its Individual Extracts by LC-MS/MS
In conclusion, the compatibility of SF and NS could reduce the bile excretion of the CNS-derived compounds, which may be one of the reasons for the enhancement of anti-myocardial ischemia after combination.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 18, 2019 Category: Drugs & Pharmacology Source Type: research

Chromatographic tools for plant-derived recombinant antibodies purification and characterization
Publication date: Available online 14 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Caterina Temporini, Raffaella Colombo, Enrica Calleri, Sara Tengattini, Francesca Rinaldi, Gabriella MassoliniAbstractIn the last two decades, plants became an interesting alternative for the production of recombinant proteins for human therapy and several antibodies expressed in plants have reached the clinical development stage. Plants are capable of post-translational modifications (PTMs) necessary for protein activity and pharmacokinetics, such as glycosylation. However, there are important kingdom-spec...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of donepezil, 6-O-desmethyl donepezil and spinosin in beagle dog plasma using liquid chromatography‒tandem mass spectrometry and its application to a drug-drug interaction study
Publication date: Available online 13 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chae Bin Lee, Jee Sun Min, Soon Uk Chae, Hye Min Kim, Jun Hee Jang, In Ho Jung, Yu Fen Zheng, Jong Hoon Ryu, Soo Kyung BaeABSTRACTSpinosin, which is traditionally used for sedation and sleep disorders, has recently shown potential effects in alleviating memory loss. As spinosin is the main bioactive component in a standardized dried 50% ethanol extract of the seeds of Zizyphus jujuba var. spinosa, a Phase Ⅱb clinical trial is ongoing, in Korea for the combination of the above extract formulated in a table...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Separation Methods hyphenated to Mass Spectrometry for the Characterization of the Protein Glycosylation at the Intact Level
Publication date: Available online 13 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julien Camperi, Valerie Pichon, Nathalie DelaunayAbstractGlycosylation is one of the most common post-translational modifications of proteins that affects their biological activity, solubility, and half-life. Therefore, its characterization is of great interest in proteomic, particularly from a diagnostic and therapeutic point of view. However, the number and type of glycosylation sites, the degree of site occupancy and the different possible structures of glycans can lead to a very large number of isoforms...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolite distribution and correlation studies of Ziziphus jujuba and Ziziphus nummularia using LC-ESI-MS/MS
Publication date: Available online 11 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Muhammad Noman Khan, Faraz Ul Haq, Saeedur Rahman, Arslan Ali, Syed Ghulam MusharrafAbstractZiziphus jujuba and Ziziphus nummularia are two important species of the genus Ziziphus. Both plants offer great commercial value and are consumed as food around the world and used for their medicinal values such as anti-inflammatory, antioxidant and hepatoprotective activities. Comprehensive chemical profiling of Z. jujuba and Z. nummularia was done through identification of major metabolites using HPLC-QTOF-MS/MS a...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

Typing and determination of SNP functional gene based on highly selective and signal-amplified fluorescence double-probe with the help of ExoIII nuclease and magnetic bead
Publication date: Available online 11 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chun-Yan Min, Lu-Qian Wu, Ting-ting Qian, Noor Ul Ain, Wen-Juan Liu, Xiao-Ning Wu, Chen Zhang, Zhe Chen, Hong-Ping XieAbstractWe have developed a fluorescence double-probe detection system with signal amplification for simple typing and determination of single nucleotide polymorphism (SNP) functional gene based on non-sequence dependence of ExoIII nuclease on dsDNA and rapid separation of magnetic bead. Matched detected gene can cyclically release abundant fluorescence-labeled ssDNA from the probe and the c...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

Advanced sensing technologies of phenolic compounds for pharmaceutical and biomedical analysis
Publication date: Available online 11 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Liya Ge, Shao-Ping Li, Grzegorz LisakAbstractPhenolic compounds are bioactive natural products of considerable interest in pharmaceuticals and biomedicines. Due to their bioactive functions, phenolic compounds have received increasing attention in recent years. Therefore, it is necessary to develop new and advanced analytical methods for determination of phenolic compounds in relation to pharmaceutical and biomedical applications. However, in majority, their detection has been largely conducted by sensitive...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

NMR Reveals an Undeclared Constituent in Custom Synthetic Peptides
This report shows how NMR analysis uncovered the unanticipated presence of substantial amounts of mannitol (20 and 43% w/w) as undeclared constituent in two custom synthetic peptides, DR and DRVYI, that were sourced commercially. Quantitative 1H NMR (qHNMR) readily detected the contaminant, even on a 60 MHz benchtop instrument, and quantified the highly polar UV-transparent adulterant. Quantum-mechanical 1H iterative Full Spin Analysis (HiFSA) achieved unambiguous identification of both the mannitol and the peptides and confirmed the quantitative results.The cases show that experimental verification supersedes trust...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 11, 2019 Category: Drugs & Pharmacology Source Type: research

Detection of wheat allergens using 2D Western blot and mass spectrometry
ConclusionsThe present study allowed the identification of different protein areas specific to these studied groups. The protein spots of interest were identified by UHPLC-MS/MS. It has been possible to establish a link between a specific symptomatology and the newly identified responsible allergens. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 11, 2019 Category: Drugs & Pharmacology Source Type: research

Combinatory high-resolution microdissection/ultra performance liquid chromatographic–mass spectrometry approach for small tissue volume analysis of rat brain glycogen
Publication date: 30 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 178Author(s): Khaggeswar Bheemanapally, Mostafa M.H. Ibrahim, Karen P. BriskiAbstractCyto-architectural diversity of brain structures emphasizes need for analytical tools for discriminative investigation of distinctive neural structures. Glycogen is the major energy reserve in the brain. There is speculation that brain utilization of this fuel source may affect detection of hypoglycemia. To evaluate sex-specific regulation of glycogen mass and mobilization in the glucose-sensory ventromedial hypothalamic nucleus (VMN), curren...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 11, 2019 Category: Drugs & Pharmacology Source Type: research

ccImproving Diagnosis of Genitourinary Cancers: Biomarker Discovery Strategies through Mass Spectrometry-based Metabolomics
Publication date: Available online 7 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Malena Manzi, Gabriel Riquelme, Nicolás Zabalegui, María Eugenia MongeAb stractThe genitourinary oncology field needs integration of results from basic science, epidemiological studies, clinical and translational research to improve the current methods for diagnosis. MS-based metabolomics can be transformative for disease diagnosis and contribute to global health parity. Metabolite panels are promising to translate metabolomic findings into the clinics, changing the current diagnosis paradigm b...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 10, 2019 Category: Drugs & Pharmacology Source Type: research

On-line In-Tube Solid Phase Microextraction coupled to Capillary Liquid Chromatography-Diode Array Detection for the analysis of caffeine and its metabolites in small amounts of biological samples
Publication date: Available online 7 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): H.D. Ponce-Rodríguez, A.A. García-Robles, P. Sáenz-González, J. Verdú-Andrés, P. Campíns-FalcóAbstractIn-tube solid phase microextraction (IT-SPME) coupled on-line to capillary liquid chromatography with diode array detection provides a simple and fast analytical methodology for the simultaneous quantitation of caffeine and its three primary metabolites (theobromine, paraxanthine and theophylline) in micro samples of serum, saliva and urine matrices. Th...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 10, 2019 Category: Drugs & Pharmacology Source Type: research

Stability behaviour of antiretroviral drugs and their combinations. 11: Characterization of interaction products of zidovudine and efavirenz, and evaluation of their anti HIV-1 activity, and physiochemical and ADMET properties
Publication date: Available online 6 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Moolchand Kurmi, Archana Sahu, Ankit Balhara, Inder Pal Singh, Smita Kulkarni, Navneet Kumar Singh, Prabha Garg, Saranjit SinghAbstractZidovudine (ZDV) and efavirenz (EFV), which belong to two separate classes of antiretroviral drugs, viz., NRTI and NNRTI, respectively, were subjected to different stability test conditions alone and in solid mixtures to evaluate possibility of interaction among them. The exposed samples were analyzed by high performance liquid chromatography (HPLC) using a C18 column and a P...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 8, 2019 Category: Drugs & Pharmacology Source Type: research

Automated Open-Access Liquid Chromatography High Resolution Mass Spectrometry to Support Drug Discovery Projects
Publication date: Available online 5 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alberto Fontana, Laura Iturrino, David Corens, Antonio L. CregoAbstractThe need of a continuous productivity increases in medicinal chemistry laboratories of the pharmaceutical industry motivated the development, over the years, of new software solutions to enable Open-Access in many analytical techniques such as NMR or LC, among others, to characterize and assess the purity of new molecules. These approaches have been widely spread in LC with low resolution MS systems, but similar automated platforms have b...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 6, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of underivatized 17 β-estradiol using a high-throughput LC–MS/MS assay – Application to support a pharmacokinetic study in ovariectomized guinea pigs
Publication date: 30 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 178Author(s): Adarsh Gandhi, Murali K. Matta, Sharron Stewart, Ashok Chockalingam, Alan Knapton, Rodney Rouse, Wendy Wu, Vikram PatelAbstractDifference in female sex hormone, β-estradiol (E2), levels can contribute to sex differences in biological processes that underlie target tissue functions (QT interval), vulnerability to diseases (hepatitis or HIV), and response toward therapies. Accurate quantification of plasma E2 level is thus an important aspect in both basic science research examining hormone-regulated physiolo...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 6, 2019 Category: Drugs & Pharmacology Source Type: research

HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants
Publication date: Available online 4 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cristina Romero Rocamora, Kalavathy Ramasamy, Siong Meng Lim, Abu Bakar Abdul Majeed, Snezana Agatonovic-KustrinAbstractA high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis (EDA) was developed to screen the antioxidant, neuroprotective and antidiabetic effects in essential oils derived from lavender flower, lemon myrtle, oregano, peppermint, sage, and rosemary leaves (Lamiaceae family). HPTLC hyphenated with microchemical (DPPH•, p-anisaldehyde, and ferric c...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Strategies for Stabilizing Formulation and QbD Assisted Development of Robust Stability Indicating Method of Azilsartan Medoxomil/Chlorthalidone
Publication date: Available online 4 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed A. Gad, Sawsan M. Amer, Hala E. Zaazaa, Said A. HassanAbstractReasons for formulation instability were investigated either encountered during production or analytical processes of azilsartan medoxomil (AZM)/chlorthalidone hydrochloride (CLT) tablets. Through the identification of the most feasible degradation pathways, several strategies were proposed to enhance the stability of AZM/CLT formulation. Furthermore, a robust HPLC-UV method was developed and validated for the determination of AZM, CLT in ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Direct and Indirect Quantification of Phosphate Metabolites of Nucleoside Analogs in Biological Samples
Publication date: Available online 3 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Nagsen Gautam, Jawaher Abdullah Alamoudi, Sushil Kumar, Yazen AlnoutiAbstractNucleoside reverse transcriptase inhibitors (NRTIs) are prodrugs that require intracellular phosphorylation to active triphosphate nucleotide metabolites (NMs) for their pharmacological activity. However, monitoring these pharmacologically active NMs is challenging due to their instability, high hydrophilicity, and their low concentrations in blood and tissues. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Characterization of CYP2B6 K262R allelic variants by quantitative allele-specific proteomics using a QconCAT standard
Publication date: Available online 3 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jill Barber, Matthew R. Russell, Amin Rostami-Hodjegan, Brahim AchourAbstractClinically-relevant proteins are routinely characterized by targeted proteomic methods, which offer high accuracy and reproducibility. However, assays developed for these techniques lack distinction between different alleles expressed in biological samples. The significance of assessing such variations in genes relevant to pharmacology will depend on their prevalence and effects on drug therapy. We propose quantitative allele-specif...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative MALDI-MS/MS assay for serum cortisol through charged derivatization
In this study, we developed a method to quantify the serum CRT by MALDI-MS/MS. This method employed the derivatization using a Girard-type reagent, 1-(hydrazinocarbonylmethyl)isoquinolinium chloride, which compensated for the lack of sensitivity. This method enabled the reproducible quantification of the serum CRT using a 20-μL sample (intra- and inter-assay relative standard deviations, 7.4% or lower), and the measurable range was 25–500 ng/mL. The serum CRT concentrations determined by the newly-developed MALDI-MS/MS method agreed well with those by liquid chromatography/electrospray ionization-MS/MS or e...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of carbapenem antibiotics using a purpose-made capillary electrophoresis instrument with contactless conductivity detection
Publication date: Available online 3 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Thi Ngoc Mai Pham, Thai Binh Le, Duc Dung Le, Tran Hung Ha, Ngoc Son Nguyen, Tien Duc Pham, Peter C. Hauser, Thi Anh Huong Nguyen, Thanh Duc Mai (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 3, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolic profiling of seminal plasma from teratozoospermia patients
In this study, the analysis of samples performed by proton nuclear magnetic resonance spectroscopy (1H NMR spectroscopy) to identify the various metabolites, with the aim of finding metabolic profiles and biomarkers related to male infertility. Eighteen de-regulated metabolites were identified in fertile men compared to teratozoospermia patients. These changes illustrate the deficiencies in absorption or metabolism of these metabolites in teratozoospermia. Furthermore, metabolic profiling showed that it is not possible to classify teratozoospermia based on teratozoospermia index (TZI). To the best of our knowledge, this is...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 3, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of eight β-lactam antibiotics in human plasma and cerebrospinal fluid by liquid chromatography coupled to tandem mass spectrometry
The objective of this work is to develop and validate a fast and reliable method using liquid chromatography coupled to tandem mass spectrometric detection to quantify simultaneously amoxicillin, cloxacillin, cefazolin, cefotaxime, ceftazidime, cefepime, meropenem and piperacillin in plasma and cerebrospinal fluid (CSF).Sample clean-up included protein precipitation with acetonitrile followed by evaporation of the supernatant and reconstitution of the residue with mobile phase solvents. Eight deuterated β-lactam antibiotics were used as internal standards. Chromatographic separation was performed on a C18 column (50&t...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Theoretical mechanistic insight into the gabapentin lactamization by an intramolecular attack: Degradation model and stabilization factors
ConclusionsThe thermodynamic stability of the lactam form (-13.63 kcal/mol) clarifies the more thermal stability of gaba-L than its related gabapentin form and the experimental preference for the lactamization. The corresponding energy profile on protonation/deprotonation forms of gabapentin indicates the pH-dependent of the process and the rate reduction in out of the mild pH.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Combinatory High-Resolution Microdissection/Ultra Performance Liquid Chromatographic-Mass Spectrometry Approach for Small Tissue Volume Analysis of Rat Brain Glycogen
Publication date: Available online 30 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Khaggeswar Bheemanapally, Mostafa M.H. Ibrahim, Karen P. BriskiAbstractCyto-architectural diversity of brain structures emphasizes need for analytical tools for discriminative investigation of distinctive neural structures. Glycogen is the major energy reserve in the brain. There is speculation that brain utilization of this fuel source may affect detection of hypoglycemia. To evaluate sex-specific regulation of glycogen mass and mobilization in the glucose-sensory ventromedial hypothalamic nucleus (VMN),...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Advanced analytical methodologies in Alzheimer’s disease drug discovery
This article reviews the analytical methodologies developed and applied in the last five years to address the early-stage tasks of the AD drug discovery process: the fast selection of active compounds (hits) and the comprehension of the ligand binding mechanism of the compound chosen to be the lead in the forthcoming development. The reviewed analytical methodologies face the most investigated pharmacological protein targets (amyloids, secretases, kinases, cholinesterases) and specific receptor- and enzyme-mediated effects in neurotransmission, neuroprotection and neurodegeneration. Some of these methodologies are notewort...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 1, 2019 Category: Drugs & Pharmacology Source Type: research