Sensitive and selective detection of puerarin based on the hybrid of reduced graphene oxide and molecularly imprinted polymer
Publication date: Available online 29 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Haifeng Li, Shuang Wang, Fan Cui, Beibei Zhuo, Shuang Wang, Chenrui Zhao, Weilu Liu (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 2, 2020 Category: Drugs & Pharmacology Source Type: research

LC-APCI+-MS/MS method for the analysis of ten hormones and two endocannabinoids in plasma and hair from the mice with different gut microbiota
Publication date: Available online 29 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Liuxi Chu, Na Li, Jia Deng, Yan Wu, Haoran Yang, Wei Wang, Dongrui Zhou, Huihua Deng (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 2, 2020 Category: Drugs & Pharmacology Source Type: research

In-depth analysis of monoclonal antibodies using microfluidic capillary electrophoresis and native mass spectrometry
Publication date: Available online 29 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sara Carillo, Craig Jakes, Jonathan Bones (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - March 2, 2020 Category: Drugs & Pharmacology Source Type: research

Serum lipidomic biomarkers for non-small cell lung cancer in nonsmoking female patients
Publication date: Available online 29 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hamada A.A. Noreldeen, Lijie Du, Wei Li, Xinyu Liu, Yanfu Wang, Guowang Xu (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 29, 2020 Category: Drugs & Pharmacology Source Type: research

Structural characterization and discrimination of Ophiopogon japonicas (Liliaceae) from different geographical origins based on metabolite profiling analysis
Publication date: Available online 27 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chao-geng Lyu, Chuan-zhi Kang, Li-ping Kang, Jian Yang, Sheng Wang, Ya-li He, Ai-ping Deng, Hong-yang Wang, Lu-qi Huang, Lan-ping Guo (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 29, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of the Acidic Species of a Monoclonal Antibody Using Free Flow Electrophoresis Fractionation and Mass Spectrometry
Publication date: Available online 27 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Suli Liu, Seth Madren, Pamela Feng, Zoran Sosic (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 29, 2020 Category: Drugs & Pharmacology Source Type: research

Evaluation of selected pharmaceuticals and personal care products in water matrix using ion trap mass spectrometry: A simple weighted calibration curve approach
Publication date: Available online 26 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chikmagalur Mallappa Gopal, Krishnamurthy Bhat, Yerabham Praveenkumarreddy, Shailesh, Virendra Kumar, Hirakendu Basu, Derrick Ian Joshua, Rakesh Kumar Singhal, Keshava Balakrishna (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 27, 2020 Category: Drugs & Pharmacology Source Type: research

Determination of isosinensetin in rat plasma by UHPLC-MS/MS: Application to oral and intravenous pharmacokinetic study in healthy rats
Publication date: Available online 26 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chao Niu, Jia Sun, Yongquan Zheng, Linlin Wang, Jian Zhang, Ruijie Chen, Weijian Ye (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 27, 2020 Category: Drugs & Pharmacology Source Type: research

Determination of 27 Amino acids’ levels in seminal plasma of asthenospermia and oligospermia patients and diagnostic value analysis
Publication date: Available online 25 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mingjie Deng, Feiyan Lin, Caiping Zhou, Yuyan Chen, Leijie Xuan, Hongzhe Wang, Tiantian Feng, Lufeng Hu (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 26, 2020 Category: Drugs & Pharmacology Source Type: research

Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products
Publication date: Available online 25 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Evelin Farsang, Davy Guillarme, Jean-Luc Veuthey, Alain Beck, Matthew Lauber, Andrew Schmudlach, Szabolcs Fekete (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 26, 2020 Category: Drugs & Pharmacology Source Type: research

Detection of allopurinol and oxypurinol in canine urine by HPLC/MS-MS: focus on veterinary clinical pharmacokinetics
Publication date: Available online 25 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ana Leonor P.C. Godoy, Clauceane de Jesus, Rafaela S. Gonçalves, Francine Johansson Azeredo, Adriana Rocha, Maria P. Marques, Vera L. Lanchote, Daniela F. Larangeira, Stella Maria Barrouin-Melo (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 26, 2020 Category: Drugs & Pharmacology Source Type: research

Estimation of passive gastrointestinal absorption and membrane retention using PAMPA test, quantitative structure-permeability and quantitative structure-retention relationship analyses of ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid and 1,3-propanediamine-N,N'-di-2-(3-cyclohexyl)propanoic acid derivatives
Publication date: Available online 25 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Biljana Tubić, Vladimir Dobričić, Jelena Poljarević, Aleksandar Savić, Tibor Sabo, Bojan Marković (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 26, 2020 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of 3’- and 6’-sialyllactose in human milk samples by HPLC-MS/MS: A validated method for the comparison of two consecutive lactation periods in the same woman
Publication date: 30 May 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 184Author(s): Orsolya Csernák, Barbara Rácz, Ágnes Alberti, Szabolcs Béni (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 26, 2020 Category: Drugs & Pharmacology Source Type: research

Umbelliprenin as a novel component of the phytochemical pool from Artemisia spp
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Serena Fiorito, Salvatore Genovese, Lucia Palumbo, Luca Scotti, Michele Ciulla, Pietro di Profio, Francesco Epifano (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

Determination of Acidity Constants for Weak Acids and Bases by Isothermal Titration Calorimetry
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lisa Samuelsen, René Holm, Audrey Lathuile, Christian Schönbeck (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantification of coproporphyrin-I and 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid in human plasma using ultra-high performance liquid chromatography coupled to tandem mass spectrometry
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yosuke Suzuki, Yuri Sasamoto, Chisato Yoshijima, Ryota Tanaka, Hiroyuki Ono, Tadasuke Ando, Toshitaka Shin, Hiromitsu Mimata, Hiroki Itoh, Keiko Ohno (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

A novel and efficient subcritical butane extraction method and UHPLC analysis of oxyprenylated phenylpropanoids from grapefruits peels
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Salvatore Genovese, Francesco Epifano, Francesca Preziuso, Azzurra Stefanucci, Luca Scotti, Tonino Bucciarelli, Pietro di Profio, Valentino Canale, Serena Fiorito (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

Dried plasma spots for therapeutic monitoring of amikacin: validation of an UHPLC-MS/MS assay and pharmacokinetic application
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Anne Caroline Cezimbra da Silva, Lilian de Lima Feltraco Lizot, Marcos Frank Bastiani, Marina Venzon Antunes, Natália Brucker, Rafael Linden (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

High-performance thin-layer chromatography linked with (bio)assays and FTIR-ATR spectroscopy as a method for discovery and quantification of bioactive components in native Australian plants
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Snezana Agatonovic-Kustrin, Ethan Doyle, Vladimir Gegechkori, David W. Morton (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

Frequentist approach for estimation of false decision risks in conformity assessment based on measurement uncertainty of liquid chromatography analytical procedures
Publication date: Available online 25 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Luciana Separovic, Felipe Rebello Lourenço (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 25, 2020 Category: Drugs & Pharmacology Source Type: research

1H HR-MAS NMR and chemometric methods for discrimination and classification of Baccharis (Asteraceae): A proposal for quality control of Baccharis trimera
Publication date: Available online 24 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Livia Macedo Dutra, Alan Diego da Conceição Santos, Allan Vinicius Felix Lourenço, Noemi Nagata, Gustavo Heiden, Francinete Ramos Campos, Andersson BarisonAbstractBaccharis trimera is a species recognized by health agencies and recommended by the Brazilian Pharmacopoeia by having medicinal properties. In this work, HR-MAS NMR spectroscopy combined with chemometric tools, such as Principal Components Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA) and Soft Independe...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 24, 2020 Category: Drugs & Pharmacology Source Type: research

Editorial for the special issue titled “Biopharmaceuticals 2020”
Publication date: Available online 22 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Szabolcs Fekete, Davy Guillarme (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 24, 2020 Category: Drugs & Pharmacology Source Type: research

Proteomic serum profile in menstrual-related and post menopause migraine
In conclusion, proteomic analysis was able to reveal differences in protein expression between migraine sufferers and non-headache women; as in other neurological diseases characterized by neuroinflammation, the serum proteome of migraine women presents an abundance of proteins indicative of cellular damage, oxidative stress and inflammation. This relevant inflammatory status, if confirmed in larger series, could represent a target for menstrual-related migraine treatment.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2020 Category: Drugs & Pharmacology Source Type: research

Evaluation of a sensitive GC–MS method to detect polysorbate 80 in vaccine preparation
Publication date: Available online 23 January 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Niraj Shende, Abhijeet Karale, Sudhakar Bhagade, Ashishkumar Gulhane, Prashant Bore, Preeti Marathe, Sourish Chakraborty, Asha D. Mallya, Rajeev M. DhereAbstractPolysorbates are the most versatile and common surfactants used as protein stabilizers. Analysis of residual polysorbate 80 (PS 80) in conjugate vaccine is challenging due to complexity of conjugate matrices and heterogeneity of the structure of the PS 80 analyte. The direct approach using high-performance liquid chromatography-evaporative light sca...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2020 Category: Drugs & Pharmacology Source Type: research

Investigation of the chemical compounds in Pheretima aspergillum (E. Perrier) using a combination of mass spectral molecular networking and unsupervised substructure annotation topic modeling together with in silico fragmentation prediction
Publication date: Available online 20 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tao-Fang Cheng, Yu-Hao Zhang, Ji Ye, Hui-Zi Jin, Wei-Dong ZhangAbstractUntargeted mass spectrometry analysis is one of the most challenging and meaningful steps in the rapid structural elucidation of the highly complex and diverse constituents of traditional Chinese medicine. Specifically, it is a laborious and time-consuming way to identify unknown compounds. Herein, a workflow was proposed to expedite the annotations of the chemical structures in Pheretima aspergillum (E. Perrier) (Di-Long, DL). First, u...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2020 Category: Drugs & Pharmacology Source Type: research

Characterization of two unknown impurities in roxithromycin by 2D LC–QTOF/MS/MS and NMR
Publication date: Available online 20 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jian Wang, Jinjin Zhou, Yu Xu, Bingqi Zhu, Yong JinAbstractTwo unknown impurities in roxithromycin were discovered and preliminarily characterized by two-dimensional liquid chromatography coupled with QTOF mass analyzer (2D LC–QTOF MS/MS). The column-switching technique of 2D LC made the chromatographic conditions in official standard of roxithromycin compatible with mass spectrometric detector. The complete MS/MS fragmentation patterns of the impurities were studied to obtain structural information ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2020 Category: Drugs & Pharmacology Source Type: research

Covalent immobilization of trypsin on polyvinyl alcohol-coated magnetic nanoparticles activated with glutaraldehyde
Publication date: Available online 20 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Selmihan Sahin, Ismail OzmenAbstractMagnetic nanoparticles were coated with polyvinyl alcohol and activated with glutaraldehyde for trypsin immobilization. The prepared magnetic nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, thermal gravimetric analysis, zeta potential meter and vibrating sample magnetometer. Free and immobilized trypsin showed optimum activity at pH 6.0, 30 °C and pH 7.0, 40 °C, respectively. Immobilized...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2020 Category: Drugs & Pharmacology Source Type: research

Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC–MS/MS
In this study, a highly sensitive and accurate LC–MS/MS method was firstly established for subsequent measurement of PdG in serum of three different female groups: thyroid cancer patients (TCs), healthy controls (HCs) and pregnant women. The factors affecting the sample preparation, MS/MS method, gradient elution program, selection of chromatographic column and internal standard (IS) have been optimized in this study. Compared with enzyme immunoassay (EIA) method, we used LC–MS/MS to shorten analysis time, increase sensitivity, raise specificity, simplify sample preparation, and reduce costs. As a result, the l...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2020 Category: Drugs & Pharmacology Source Type: research

Optimization of a High-Throughput Fluorescence Polarization Assay for STAT5B DNA Binding Domain-Targeting Inhibitors
Publication date: Available online 19 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Pimyupa Manaswiyoungkul, Fettah Erdogan, Olasunkanmi O. Olaoye, Aaron D. Cabral, Elvin D. de Araujo, Patrick T. GunningAbstractSignal transducer and activator of transcription 5B (STAT5B) is constitutively activated in multiple cancers as a result of hyperactivating mutations or dysregulation of upstream effectors. Therapeutic strategies have predominantly targeted the Src homology 2 (SH2) domain to inhibit STAT phosphorylation, a prerequisite for STAT5B transcriptional activation. An alternative approach ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2020 Category: Drugs & Pharmacology Source Type: research

Study of chromatographic behavior of antibiotic drugs and their metabolites based on quantitative structure-retention relationships with the use of HPLC-DAD
Publication date: Available online 19 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Justyna Walczak, Małgorzata Szultka-Młyńska, Katarzyna Pauter, Bogusław BuszewskiAbstractThe separation of eleven antibiotics and ten metabolites were studied using high performance liquid chromatography. The C18-PFP octadecyl with integral PFP, C18-AR octadecyl with integral phenyl, C18-HL octadecyl and phenyl phase were used as stationary phases. Mixtures of acetontrile–0.1% formic acid in water were investigated as mobile phases. The elution order of the target compounds was similar for all fo...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2020 Category: Drugs & Pharmacology Source Type: research

The chemical transformations for Radix Astragali via different alkaline wash conditions by quantitative and qualitative analyses
In conclusion, this work would provide a scientific and practical method for quality control of Radix Astragali as well as its compound preparations.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2020 Category: Drugs & Pharmacology Source Type: research

Comprehensive chemotaxonomic analysis of saffron crocus tepal and stamen samples, as raw materials with potential antidepressant activity
Publication date: Available online 18 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Javad Mottaghipisheh, Mohammad Mahmoodi Sourestani, Tivadar Kiss, Attila Horváth, Barbara Tóth, Mehdi Ayanmanesh, Amin Khamushi, Dezso CsuporAbstractSaffron crocus (Crocus sativus L.) has been widely grown in Iran. Its stigma is considered as the most valuable spice for which several pharmacological activities have been reported in preclinical and clinical studies, the antidepressant effect being the most thoroughly studied and confirmed. This plant part contains several characteristic second...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 19, 2020 Category: Drugs & Pharmacology Source Type: research

Investigating the molecular structure of placenta and plasma in pre-eclampsia by infrared microspectroscopy
In this study, IR microspectroscopy was applied to identify molecular changes associated with the pathogenesis of PE in placental tissues and plasma samples from pre-eclamptic women and normotensive matched controls. The obtained spectra were analyzed by multivariate analysis in the spectral ranges of 3050–2800 cm−1 and 1855–1485 cm−1 corresponding to lipid and protein-carbonyl components, respectively. In the lipid region, an increase in CH2/CH3 ratio was noticed and higher level of unsaturation index in placenta was evident. New lipid species emerged as a consequence of oxidative str...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 19, 2020 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of 3’- and 6’- sialyllactose in human milk samples by HPLC-MS/MS: a validated method for the comparison of two consecutive lactation periods in the same woman
Publication date: Available online 19 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Orsolya Csernák, Barbara Rácz, Ágnes Alberti, Szabolcs BéniAbstractHuman milk oligosaccharides (HMOs) are complex carbohydrates that exist naturally in mother’s milk. Due to their myriad beneficial biological effects, HMOs are in the focus of current researches. Some of these complex carbohydrates are already commercially available utilizing their health-promoting benefits not just in infant formula but in dietary supplements. Among the acidic oligosaccharides 3’- an...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 19, 2020 Category: Drugs & Pharmacology Source Type: research

Validation of an Assay for Quantifying Ganciclovir in Dried Blood Spots
We describe a simple method for determining systemic GCV concentrations using dried blood spot (DBS) samples. GCV was extracted from a single 6 mm punch via sonication in methanol, then quantified using liquid chromatography-tandem mass spectrometry. The assay was accurate and precise in the dynamic range of 10 to 10,000 ng/mL. GCV concentrations determined in DBS agreed well with GCV concentrations observed in serum. The assay was successfully applied to patient samples, and will be used to support pharmacokinetic studies in an ongoing clinical trial of VGC in infants with CMV-mediated hearing loss. (Source:...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 18, 2020 Category: Drugs & Pharmacology Source Type: research

Development of an ICP-MS/MS approach for absolute quantification and determination of phosphodiester to phosphorothioate ratio in therapeutic oligonucleotides
Publication date: Available online 15 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Juliusz Bianga, Magali Perez, Damien Mouvet, Caroline Cajot, Philippe De Raeve, Arnaud DelobelAbstractA new analytical method based on ICP-MS/MS is proposed for the characterization of synthetic phosphorothioate oligonucleotides. Absolute quantification of oligonucleotides is challenging, as well as the determination of phosphodiester to phosphorothioate ratio for phosphorothioate oligonucleotides. Both are considered as critical quality attributes and should be determined using robust validated methods. T...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 16, 2020 Category: Drugs & Pharmacology Source Type: research

Magnetic solid phase extraction followed with LC-MS/MS for determination of glimepiride in beagle dog plasma and its application to bioequivalence study
In this study, the core-shell polydopamine-coated magnetic nanomaterials were synthesized with one-step approach and applied as magnetic solid phase extraction adsorbents combined with LC-MS/MS for quantification of the diabetes drug glimepiride in beagle dog plasma. The Fe3O4@PDA nanomaterials have strong magnetic response, good dispersibility in aqueous solution, and numerous binding sites for π–π interaction and hydrogen bonding with glimepiride. Based on these merits, glimepiride could be quickly extracted and separated from plasma within 10min. The established method has good linearity (1-2000ng•mL-1)...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 16, 2020 Category: Drugs & Pharmacology Source Type: research

UPLC-MS identification and anticomplement activity of the metabolites of Sophora tonkinensis flavonoids treated with human intestinal bacteria
Publication date: Available online 14 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xin Jin, Yan Lu, Shaoxin Chen, Daofeng ChenAbstractAnticomplement activity played an important role in anti-inflammatory effects of traditional Chinese herbs. The total flavonoids of Sophora tonkinensis (TFST) were inactive on the complement system but showed obvious anticomplement activity after incubated with human intestinal bacteria in vitro. In order to discover the metabolic activation of TFST by intestinal flora, the constituents of TFST and its metabolites were identified by UPLC-ESI-LTQ/MS. Their ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Metabolomic approach for rapid differentiation of Fritillaria bulbs by matrix-assisted laser desorption/ionization mass spectrometry and multivariate statistical analysis
Publication date: Available online 14 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ze Wang, Hanyi Xie, Juan Ren, Yang Chen, Xiaofei Li, Xiangfeng Chen, Tak-Wah Dominic ChanAbstractThe bulbs of Fritillaria have been used for centuries as food and medicinal products in many Asian countries. Different Fritillaria species have distinct pharmacological effects despite of their similar appearances. Effective differentiation of Fritillaria species can avoid adulteration and is crucial to its clinical uses. In this paper, a hybrid method of matrix assisted laser desorption/ionization mass spectr...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Bridging Size and Charge Variants of a Therapeutic Monoclonal Antibody by Two-Dimensional Liquid Chromatography
Publication date: Available online 14 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhi Chen, Ming Zeng, Sung-Joo Park, Gurusamy Balakrishnan, Kaimeng Zhou, Duohai Pan, Tapan K.AbstractMonoclonal antibodies are heterogeneous in nature and may contain numerous variants with differences in size, charge, and hydrophobicity, which may impact clinical efficacy, immunogenicity, and safety. Characterization of antibody variants is necessary to build structure-function correlation and establish a proper control strategy. Isolation and enrichment of variants by conventional chromatographic peak fr...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Inside gel electromembrane extraction: A novel green methodology for the extraction of morphine and codeine from human biological fluids
Publication date: Available online 14 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Atyeh Rahimi, Saeed Nojavan, Hadi TabaniAbstractIn this work, a new mode of gel-electromembrane extraction (G-EME), called “inside” gel-EME (IG-EME) is proposed for the extraction of morphine and codeine as model basic drugs from complex biological samples. Here, an aqueous media that was captured inside the agarose gel membrane, acted as both gel membrane and the acceptor phase (AP) at the same time. In this regard, the membrane served as the separation filter (membrane) and supported liquid a...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Fast simultaneous quantification of gabapentin and cetirizine in cell lysates by means of HPLC-MS/MS
Publication date: Available online 13 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lukas Schlatt, Ana Carolina Conchon Costa, Vivien Barz, Giuliano Ciarimboli, Uwe KarstAbstractA high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was successfully developed for the simultaneous detection of gabapentin (GBP) and cetirizine (CTZ) in cell lysates. Multiple reaction monitoring (MRM) transitions were found for GBP, CTZ and deuterated internal standards of both, allowing a selective and sensitive detection. Limits of detection (LODs) of 0.04 ng/mL and 0.0...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Conventional and nonconventional extraction techniques for optimal extraction processes of rosmarinic acid from six Lamiaceae plants as determined by HPLC-DAD measurement
Publication date: Available online 13 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Beatrix Sik, Erika Lakatos Hanczné, Viktória Kapcsándi, Zsolt AjtonyAbstractThe goal of this study was to improve the extraction efficiency of rosmarinic acid (RA) from Lamiaceae herbs (lemon balm, peppermint, oregano, rosemary, sage, and thyme) using various extraction techniques (maceration with stirring, MACS; heat reflux, HRE; and microwave-assisted extraction, MAE) and extraction conditions (solvent acidity, solvent type, extraction time and temperature). The RA content was measur...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Corrigendum to “LC–MS/MS method for the simultaneous analysis of seven antimalarials and two active metabolites in dried blood spots for applications in field trials: Analytical and clinical validation” [J. Pharm. Biomed. Anal. 154 (2018) 263–277]
Publication date: Available online 13 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Joanna Gallay, Sylvain Prod’hom, Thomas Mercier, Carine Bardinet, Dany Spaggiari, Emilie Pothin, Thierry Buclin, Blaise Genton, Laurent Arthur Decosterd (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Mass spectrometry-based methods in characterization of the higher order structure of protein therapeutics
Publication date: Available online 12 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Igor A. Kaltashov, Cedric E. Bobst, Jake Pawlowski, Guanbo WangAbstractHigher order structure of protein therapeutics is an important quality attribute, which dictates both potency and safety. While modern experimental biophysics offers an impressive arsenal of state-of-the-art tools that can be used for the characterization of higher order structure, many of them are poorly suited for the characterization of biopharmaceutical products. As a result, these analyses were traditionally carried out using class...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

Universal quantification method of degradation impurities in 16-membered macrolides using HPLC-CAD and study on source of the impurities
In this study, a reliable method was established for the analysis of impurities in 16-membered macrolides for the first time by high performance liquid chromatography tandem with charged aerosol detector (HPLC-CAD). The chromatographic conditions and CAD parameters were optimized for a good separation and sensitivity. The impurities were identified by ion trap time-of-flight mass spectrometry (IT-TOF/MS), the developed method was validated, and the sources of degradation impurities in production were investigated. Based on the experimental results, the superiority of the developed method over UV detection was demonstrated ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS
In this study, a highly sensitive and accurate LC-MS/MS method was firstly established for subsequent measurement of PdG in serum of three different female groups: thyroid cancer patients (TCs), healthy controls (HCs) and pregnant women. The factors affecting the sample preparation, MS/MS method, gradient elution program, selection of chromatographic column and internal standard (IS) have been optimized in this study. Compared with enzyme immunoassay (EIA) method, we used LC-MS/MS to shorten analysis time, increase sensitivity, raise specificity, simplify sample preparation, and reduce costs. As a result, the linear range ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

Sensitive and selective quantification of mid-regional proadrenomedullin in human plasma using ultra-performance liquid chromatography coupled with tandem mass spectrometry
Publication date: Available online 11 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Motoshi Iwao, Yosuke Suzuki, Ryota Tanaka, Teruhide Koyama, Etsuko Ozaki, Takeshi Nakata, Kohei Aoki, Akihiro Fukuda, Yuhki Sato, Nagato Kuriyama, Naoya Fukunaga, Fuminori Sato, Fumihiko Katagiri, Keiko Ohno, Hirotaka Shibata, Hiromitsu Mimata, Hiroki ItohAbstractMid-regional pro-adrenomedullin (MR-proADM) is suggested to be a prognostic indicator for various diseases. Plasma MR-proADM concentration is commonly measured using immunoassays based on its immunochemical characteristics. However, some immunolog...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Determination of size variants by CE-SDS for approved therapeutic antibodies: key implications of subclasses and light chain specificities
Publication date: Available online 10 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Elsa Wagner, Olivier Colas, Stéphane Chenu, Alexandre Goyon, Amarande Murisier, Sarah Cianferani, Yannis François, Szabolcs Fekete, Davy Guillarme, Valentina D’Atri, Alain BeckAbstractIn the present work, a generic non-reducing capillary electrophoresis sodium dodecyl sulphate (nrCE-SDS) method was tested for a wide range of 26 FDA and EMA approved monoclonal antibodies (mAbs) and 2 antibody drug conjugates (ADCs) as well as for the NISTmab, in a QC environment (e.g. testing quality req...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Finding a reliable limit of detection in the NIR determination of residual moisture in a freeze-dried drug product
Publication date: Available online 11 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gloria Clua-Palau, Enric Jo, Sasha Nikolic, Jordi Coello, Santiago MaspochAbstractThe validation of an analytical method in the pharmaceutical industry follows strictly regulated guidelines. The introduction of multivariable calibration methods requires a revision of these recommendations, since some of them are contradictory regarding the limit of detection (LOD). This work compares the LOD values obtained using pseudounivariate and multivariate procedures in the PLS-NIR determination of residual moisture...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 12, 2020 Category: Drugs & Pharmacology Source Type: research