A high expression Mas-related G protein coupled receptor X2 cell membrane chromatography coupled with liquid chromatography and mass spectrometry method for screening potential anaphylactoid components in kudiezi injection
Publication date: Available online 17 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yuanyuan Lin, Yanni Lv, Jia Fu, Qianqian Jia, Shengli HanAbstractKudiezi injection is a traditional Chinese medicine injection used widely in China to alleviate blood stasis and to stimulate blood circulation. Its use has been associated with a high rate of adverse effects including anaphylactoid reactions. In the present study, a two-dimensional system comprised of high expression Mas-related G protein-coupled receptor X2 cell membrane chromatography coupled online with HPLC-ESI-IT-TOF-MS was established, and...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a (RP)UHPLC-UV-(HESI/Orbitrap)MS method for the identification and quantification of mixtures of intact therapeutical monoclonal antibodies using a monolithic column
Publication date: Available online 17 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Raquel Pérez-Robles, Luis Cuadros-Rodríguez, Antonio Salmerón-García, Natalia NavasAbstractMonoclonal antibodies (mAbs) are one of the most important types of biopharmaceutics and have proved enormously successful in the treatment of cancers and autoimmune diseases. In this paper, we present a fast, straightforward reversed phase (RP)UHPLC-UV-(HESI/Orbitrap) MS method for the separation and identification of five of the most commonly used mAbs, i.e. bevazizumab (BEV), cetuximab (CTX...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

STAT3 Differential Scanning Fluorimetry and Differential Scanning Light Scattering Assays: addressing a missing link in the characterization of STAT3 Inhibitor interactions
Publication date: Available online 17 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Matthieu Desroses, Sander Busker, Juan Astorga-Well, Sanaz Attarha, Iryna kolosenko, Roman Zubare, Thomas Helleday, Dan Grandér, Brent D.G. PageAbstractSTAT3 protein is an established target for the development of new cancer therapeutic agents. Despite lacking a traditional binding site for small molecule inhibitors, many STAT3 inhibitors have been identified and explored for their anti-cancer activity. Because STAT3 signaling is mediated by protein-protein interactions, indirect methods are often emplo...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

Recent progress on the sensitive detection of cardiovascular disease markers by electrochemical-based biosensors
Publication date: Available online 17 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Nurgul K. Bakirhan, Goksu Ozcelikay, Sibel A. OzkanAbstractCardiovascular disease is the most reason for deaths in all over the world. Hence, biomarkers of cardiovascular diseases are very crucial for diagnosis and management process. Biomarker detection demand is opened the important way in biosensor development field. Rapid, cheap, portable, precise, selective and sensitive biomarker sensing devices are needed at this point to detect and predict disease. A cardiac biomarker can be orderable as C-reactive pro...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

Detection and quantification of Enterococcus faecalis RNPP-type quorum sensing peptides in bacterial culture media by UHPLC-MS
Publication date: Available online 17 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Frederick Verbeke, Nathan Debunne, Yorick Janssens, Liesa Tack, Evelien Wynendaele, Petra Rigole, Tom Coenye, Bart De SpiegeleerAbstractBacteria communicate with each other using quorum sensing; i.e. the production and sensing of signalling molecules. Enterococcus faecalis, a Gram-positive bacterium, employs peptides as quorum sensing molecules. These peptides have previously been isolated from culture media by elaborate, time and medium-consuming sample preparation approaches and specific bacteria-based bio-s...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

Development of a disposable electrochemical sensor for detection of cholesterol using differential pulse voltammetry
In this study, a sensitive and selective electrochemical sensor was fabricated by using a screen printed carbon electrode (SPCE), multi-walled carbon nanotubes (MWCNTs) and β-cyclodextrin (β-CD) for detecting cholesterol. MWCNTs were functionalized with benzoic acid moiety by employing diazonium salt chemistry, and, subsequently, a thin film of functionalized CNTs were coated on the surface of SPCE. Afterwards, β-CD was immobilized on functionalized MWCNTs modified SPCE which acts as a host to recognize guest (cholesterol) molecule specifically. Under the optimal experimental conditions and using differentia...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of five bioactive components of Gancao in rat plasma by UHPLC-MS/MS and its application to comparative pharmacokinetic study of incompatible herb pair Gansui-Gancao and Gansuibanxia Decoction
Publication date: 10 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 159Author(s): Yue Cui, Ting Liu, Ye Zhang, Roujia Wang, Xiaozhou Liu, Qili Zhang, Peipei Yu, Yunli Zhao, Zhiguo YuAbstractIncompatible herb pair Gansui-Gancao is recorded in “eighteen incompatible” medicaments in many monographs of TCM (Traditional Chinese Medicine) which means the two herbs can not be co-used in most cases. However, Gansuibanxia decoction composed of Gansui(Kansui), Banxia(Pinellia), Shaoyao(Peony) and Gancao(Liquorice) is a traditional Chinese formula which has been clinically employed for the...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 15, 2018 Category: Drugs & Pharmacology Source Type: research

Microfluidics contribution to pharmaceutical sciences: from drug discovery to post marketing product management
Publication date: Available online 11 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gwenaël Nys, Marianne FilletAbstractDrug discovery and development is a long-lasting process in which many challenges have to be addressed at every stage, from the discovery of the target biomolecule to the commercialization of the discovered drugs and its quality control. From thousands of hits identified during early drug discovery only one drug is eventually efficient and safe enough to be commercialized. This high rejection rate, especially during preclinical and clinical studies have led to an expone...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 12, 2018 Category: Drugs & Pharmacology Source Type: research

Application of salting-out thin layer chromatography in computational prediction of minimum inhibitory concentration and blood-brain barrier penetration of some selected fluoroquinolones
Publication date: Available online 11 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Azza H. Rageh, Noha N. Atia, Hamdy M. Abdel-RahmanAbstractThe 2017 FDA safety review regarding the CNS (central nervous system) side effects associated with the systemic use of fluoroquinolones antibacterials (FQs) was the key motivation to carry out this work. The main objective of this study is to investigate lipophilicity and retention parameters of some selected fluoroquinolones antibacterials (FQs) namely; levofloxacin (LEV), ofloxacin (OFL), gatifloxacin (GAT), norfloxacin (NOR), sparfloxacin (SPA), cipr...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 12, 2018 Category: Drugs & Pharmacology Source Type: research

Fluorescence polarization immunoassay of colchicine
In this study, a fluorescence polarization immunoassay (FPIA) technique was developed to determine colchicine (COL), an alkaloid of noxious plants of the order Liliales that is used in a number of medications to treat gout. An optimal combination of the polyclonal antibody and the antigen labelled with fluorescein isothiocyanate (FITC) was selected. Conditions for the competitive interaction of the antigen in the tested samples and its fluorophore conjugate (COL–FITC) with anti-COL antibodies were optimised, and the analytical characteristics of the assay were determined. The developed FPIA was characterised by a det...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 11, 2018 Category: Drugs & Pharmacology Source Type: research

A liquid chromatography high-resolution mass spectrometry in vitro assay to assess metabolism at the injection site of subcutaneously administered therapeutic peptides
Publication date: Available online 10 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Simone Esposito, Maria Lucia de Leonibus, Raffaele Ingenito, Elisabetta Bianchi, Laura Orsatti, Edith MonteagudoAbstractSubcutaneous (SC) injection is the most common administration route for peptide therapeutics. Catabolism at the injection site can be a specific and major degradation pathway for many SC administered peptides. In some cases, it can significantly affect pharmacokinetics, particularly bioavailability, and have detrimental effects on the efficacy of the drug.This work describes a liquid chromato...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 11, 2018 Category: Drugs & Pharmacology Source Type: research

A new descriptor via bio-mimetic chromatography and modeling for the blood brain barrier (Part II)
Publication date: Available online 25 May 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maria G. Kouskoura, Aikaterini I. Piteni, Catherine K. MarkopoulouAbstractWithin the context of drug design methodology for the central nervous system (CNS), a predictive model which can shorten the process of finding new candidate drugs was developed. Therefore, the retention time of 51 molecules which are clinically established to enter the blood brain barrier (BBB), were recorded on two HPLC columns. For this purpose, a lipophilic butyl (C4) stationary phase was used to simulate the behavior of a drug regard...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantification of EVT201, a novel partial positive allosteric GABAA receptor modulator, and its two metabolites in human plasma by UHPLC/MS/MS
Publication date: Available online 1 June 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xianhua Zhang, Congya Zhou, Yuanyuan Zhang, Shuangpeng Zhu, Xiaofeng Cai, Chunmiao Pan, Chuanzhen Wang, Suodi Zhai, Li YangAbstractA sensitive, accurate and rapid UHPLC-MS/MS method was developed and validated for the simultaneous determination of EVT201 and its two metabolites, Ro46-1927 and Ro18-5528, in human plasma. D6-EVT201 was used as the internal standard (IS). Plasma samples were extracted using ethyl acetate after being alkalized with saturated sodium carbonate solution. Chromatographic separation was...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Targeted analysis of microbial-generated phenolic acid metabolites derived from grape flavanols by gas chromatography-triple quadrupole mass spectrometry
Publication date: Available online 20 June 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Eileen Carry, Danyue Zhao, Ilaria Mogno, Jeremiah Faith, Lap Ho, Tom Villani, Harna Patel, Giulio M. Pasinetti, James E. Simon, Qingli WuAbstractGrape-derived products contain a wide array of bioactive phenolic compounds which are of significant interest to consumers and researchers for their multiple health benefits. The majority of bioavailable grape polyphenols, including the most abundant flavan-3-ols, i.e. (+)-catechin and (-)-epicatechin, undergo extensive microbial metabolism in the gut, forming metabol...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

In-vitro Metabolism, CYP Profiling and metabolite identification of E- and Z- guggulsterone, a potent hypolipidmic agent
Publication date: Available online 24 June 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yashpal S. Chhonker, Hardik Chandasana, Veenu Bala, Rao Mukkavilli, Deepak Kumar, Subrahmanyam Vangala, Rabi S. BhattaAbstractThe polyphenol E- and Z-gugggulsterone (GS) is an antagonist ligand for the Farnesoid X Receptor (FXR) and known to possess potent hypolipidemic properties as shown in various preclinical and clinical studies. In the present study, we examined drug-like properties of GS by assessing the isomers plasma protein binding, metabolic stability, CYP profiling, CYP inhibition, and phase I and I...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Trigonelline inhibits intestinal microbial metabolism of choline and its associated cardiovascular risk
This study demonstrates the effect of trigonelline on gut microbiota responsible for choline metabolism and this can be used as a model for evaluation of herbal drugs and its effect in gut microbiota prompted cardiovascular disorders.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of the cyclic peptide GG-8-6 in rat plasma using LC-MS/MS and its application to a pharmacokinetic study
Publication date: Available online 30 June 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jie-Tao Chen, Xiao-Min Guo, Ping Yang, Qing MuAbstractHepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide. The discovery of new anticancer compounds is of great significance. GG-8-6, cyclo-(Val1-Leu2-Pro3-Ile4-Leu5-Leu6-Leu7-Val8-Leu9), a new synthetic cyclic peptide, might be a potential candidate for developing new anti-HCC drugs. GG-8-6 shares no structural homology to current anti-HCC drugs. Therefore, it was necessary to develop a quantitative method for the determ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring
Publication date: Available online 30 June 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Enrico Tenaglia, Anna Ferretti, Laurent A. Decosterd, Dominique Werner, Thomas Mercier, Nicolas Widmer, Thierry Buclin, Carlotta GuiducciAbstractThe use of DNA aptamers in biosensors for the quantification of pharmaceuticals in the clinics would help to overcome the limitations of antibody-based detection for small molecules. The interest for such systems is proven by the ever-increasing number of aptamer-based solutions for analytics proposed in the literature as proof-of-concept demonstrators. Despite such d...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

The Development of Simple Flow Injection Analysis Tandem Mass Spectrometric Methods for the Cutaneous Determination of Peptide-Modified Cationic Gemini Surfactants Used as Gene Delivery Vectors
Publication date: Available online 2 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mays Al-Dulaymi, Deborah Michel, Jackson M. Chitanda, Ildiko Badea, Anas El-AneedAbstractDiquaternary ammonium gemini surfactants are a class of non-viral gene delivery vectors, primarily studied for their dermal applications. However, their biological fate has rarely been investigated. In this work, we developed simple flow injection analysis tandem mass spectrometric methods, (FIA)-MS/MS, to understand the fate and biodistribution of topically applied gemini surfactant-based therapeutics in an ex-vivo skin mo...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Combining excitation-emission matrix fluorescence spectroscopy, Parallel Factor Analysis, cyclodextrin-modified micellar electrokinetic chromatography and Partial Least Squares Class-Modelling for green tea characterization
In this study, an alternative analytical approach for analyzing and characterizing green tea (GT) samples is proposed, based on the combination of excitation–emission matrix (EEM) fluorescence spectroscopy and multivariate chemometric techniques. The three-dimensional spectra of 63 GT samples were recorded using a Perkin–Elmer LS55 luminescence spectrometer; emission spectra were recorded between 295 and 800 nm at excitation wavelength ranging from 200 to 290 nm, with excitation and emission slits both set at 10 nm. The excitation and emission profiles of two factors were obtained using Parallel Factor ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Development, validation and application of a novel HPLC-MS/MS method for the quantification of atorvastatin, bisoprolol and clopidogrel in a large cardiovascular patient cohort
Publication date: Available online 3 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Richard Myles Turner, Vanessa Fontana, Mark Bayliss, Sarah Whalley, Anahi Santoyo Castelazo, Munir PirmohamedAbstractCardiovascular disease is a leading cause of morbidity, mortality, and healthcare expenditure worldwide. Importantly, there is interindividual variation in response to cardiovascular medications, leading to variable efficacy and adverse events. Therefore a rapid, selective, sensitive and reproducible multi-analyte HPLC-MS/MS assay for the quantification in human plasma of atorvastatin, its major ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of Degradation Products of Praziquantel during the Mechanochemical Activation
Publication date: Available online 5 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ivana Šagud, Debora Zanolla, Beatrice Perissutti, Nadia Passerini, Irena ŠkorićAbstractPraziquantel (PZQ) is an inexpensive, low toxicity BCS II class anthelmintic drug used for the treatment of neglected tropical diseases. In earlier papers a mechanochemical activation has been used to induce physical transformations on the drug which would ameliorate its solubility and hence its bioavailability and a systematic study of the effects of varying temperature, frequency and time of milling on drug m...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Chemical markers for quality control of bran-fried sulfur-fumigated Paeoniae Radix Alba
Publication date: Available online 5 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ya-Yun Xu, Fang Long, Ye-Qing Zhang, Jin-Di Xu, Ming Kong, Song-Lin LiAbstractIn traditional Chinese medicine practice, crude herbs are often subjected to traditional processing (Paozhi in Chinese) for a special medicinal purpose. Bran-frying is one of processing methods for Paeoniae Radix Alba (PRA). Previous studies found that paeoniflorin and paeoniflorin sulfonate, a principle bioactive compound and its sulfur-fumigation induced characteristic sulfur-containing derivative, could be used together with sulfur...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Discovery of quality control markers from traditional Chinese medicines by fingerprint-efficacy modeling: Current status and future perspectives
Publication date: Available online 8 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cai Zhang, Xian Zheng, Hui Ni, Ping Li, Hui-Jun LiAbstractQuality control (QC) is crucial for ensuring the safety and effectiveness of traditional Chinese medicines (TCMs). Due to extremely complicated phytochemical matrices and characteristic mode of “multi-component, multi-target, integrated adjustment”, discovering of QC markers from TCMs is a big challenge. Fingerprint-efficacy (FE) modeling method is currently proposed as an effective and reasonable attempt. This review summarizes the methodolo...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a simple chromatographic method for simultaneous determination of clindamycin phosphate and rifampicin in skin permeation studies
Publication date: Available online 10 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maira N. Pereira, Breno N. Matos, Tais Gratieri, Marcilio Cunha-Filho, Guilherme M. GelfusoAbstractRifampicin (RIF) and clindamycin phosphate (CDM) are the main drugs currently used in combination to treat severe infectious diseases in hair follicles. This work describes a simple, rapid and sensitive method for simultaneous analysis of RIF and CDM in the different skin layers using high performance liquid chromatography (HPLC). The efficient chromatographic separation of CDM and RIF was succeeded using a C18 c...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

New developments and possibilities of wide-pore superficially porous particle technology applied for the liquid chromatographic analysis of therapeutic proteins
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Balázs Bobály, Jean-Luc Veuthey, Davy Guillarme, Szabolcs FeketeAbstractThis review paper discusses the success of columns packed with superficially porous particles (SPP) in liquid chromatography for the analysis of peptides and proteins. First, it summarizes the history of SPP, including the development of different SPP generations from particles of 50 μm to sub-2 μm. It also critically discusses the improved kinetic performance of SPP particles in comparison to fully porous particles....
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Quantification of nucleotides and their sugar conjugates in biological samples: Purposes, instruments and applications
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Xuan Qin, Xin WangAbstractNucleotides and their sugar conjugates are fundamental molecules in life, participating in processes of DNA/RNA composition, cell wall build-up, glycosylation reactions, and signal conduction. Therefore, the quantification of these compounds in biological samples significantly benefits the understanding of their functions. However, nucleotides and nucleotide sugars are extremely hydrophilic, causing bad retention and peak symmetry on regular C18 chromatographic columns. To solve this p...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Recent advances in the capillary electrophoresis analysis of antibiotics with capacitively coupled contactless conductivity detection
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Prasanta Paul, Cari Sänger-van de Griend, Erwin Adams, Ann Van SchepdaelAbstractThis review describes briefly the high rate of counterfeiting of antimicrobial drugs with focus upon its immediate health consequences. The major part of this review encompasses accounts of the improvements achieved in the domain of miniaturization of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The application of this principle into the development of portable devices as well...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of tryptophan, kynurenine, kynurenic acid and two monoamines in rat plasma by HPLC-ECD/DAD
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Ting-ting Du, Ting Cui, Hong-mei Qiu, Nian-ru Wang, Dan Huang, Xin-hui JiangAbstractA high-performance liquid chromatography method with a diode array and an electrochemical detection (HPLC-ECD/DAD) was developed to determine the levels of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in rat plasma. The prepared samples were separated on a BDS column (4.6 mm × 250 mm, 5 mm) with column oven temperature of 25 °C....
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Bioanalysis of free and liposomal Amphotericin B in rat plasma using solid phase extraction and protein precipitation followed by LC-MS/MS
In this study, an original strategy was developed to separately determine F-AMB and L-AMB in rat plasma using LC–MS/MS. F-AMB was analyzed after separation by solid phase extraction, total AMB (T-AMB) was determined after protein precipitation and L-AMB was determined by difference. The method was fully validated. Calibration curves were linear in the ranges 0.7–120 μg/mL for T-AMB and 0.2–20 μg/mL for F-AMB. Accuracy and precision results were within acceptable variability limits, recoveries were consistent and reproducible, matrix effects were insignificant and analytes were stable under all t...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Efficient extraction of proanthocyanidin from Ginkgo biloba leaves employing rationally designed deep eutectic solvent-water mixture and evaluation of the antioxidant activity
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Jun Cao, Luyao Chen, Mohan Li, Fuliang Cao, Linguo Zhao, Erzheng SuAbstractProanthocyanidin (PAC) holds strong free radicals scavenging ability and is widely used as oxygen free radical scavenger. Huge demand, safety and economic aspect are forcing the PAC extraction to explore new resource and greener solvent. An efficient extraction method of PAC from Ginkgo biloba leaves was established by employing deep eutectic solvent (DES)-water mixture. After a rational design, a DES-water mixture (named as Ch-M55) was ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Sandwich pair nanobodies, a potential tool for electrochemical immunosensing serum prostate-specific antigen with preferable specificity
In this study, a sandwich pair of nanobodies (Nbs) (i.e., Nb2 and Nb40) against PSA surface antigen was obtained from an alpaca-derived immune phage display library. A sandwich-type immunosensor for the sensitive and selective detection of PSA in serum samples was ingeniously designed based on the pair of Nbs. The small size of Nb40 allowed high capture densities on the surface of reduced graphene oxide (rGO) nanocomposed with massive Au nanoparticles (rGO@AuNPs), which significantly improved the conductivity and provided a large area to anchor many primary antibodies. The secondary antibody Nb2 fused with streptavidin -bi...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

On-line electrochemistry/electrospray ionization mass spectrometry (EC-ESI-MS) system for the study of nucleosides and nucleotides oxidation products
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Sylwia Studzińska, Lena Siecińska, Bogusław BuszewskiAbstractThe main aim of present investigation was to study the oxidation products of nucleosides and nucleotides with the use of on-line electrochemistry/electrospray ionization mass spectrometry (EC-ESI-MS) system. The conditions applied in the system were optimized in complex manner, involving study of the impact of working electrodes or sample solvent on the oxidation of tested compounds and their ionization in mass spectrometry. Finally 5 mM of ammon...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Gold nanoparticles coupled with graphene quantum dots in organized medium to quantify aminoglycoside anti-biotics in yellow fever vaccine after solid phase extraction using a selective imprinted polymer
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Carlos A.T. Toloza, Joseany M.S. Almeida, Sarzamin Khan, Yasmin G. dos Santos, Andrea R. da Silva, Eric C. Romani, Dunieskys G. Larrude, Fernando L. Freire, Ricardo Q. AucélioAbstractThe determination of kanamycin sulfate was made indirectly by measuring its effect on photoluminescent amino functionalized graphene quantum dots (GQDs-amino) associated with gold nanoparticles (AuNPs) that were produced by the reduction of AuCl4 with NaBH4 in an aqueous dispersion of GQDs-amino (obtained by the pyrolysis of...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Interval importance index to select relevant ATR-FTIR wavenumber Intervals for falsified drug classification
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): A. Kahmann, M.J. Anzanello, F.S. Fogliatto, W.A. Chaovalitwongse, M.C.A. Marcelo, M.F. Ferrão, R.S. Ortiz, K.C. MariottiAbstractThe commerce of falsified drugs has substantially grown in recent years due to facilitated access to technologies needed for copying authentic pharmaceutical products. Attenuated Total Reflectance coupled with Fourier Transform Infrared (ATR-FTIR) spectroscopy has been successfully employed as an analytical tool to identify falsified products and support legal agents in interrup...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitation of polymorphic impurity in entecavir polymorphic mixtures using powder X-ray diffractometry and Raman spectroscopy
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Yanlei Kang, Zhanying Shao, Qiang Wang, Xiurong Hu, Dongdong YuAbstractEntecavir was used for the treatment of chronic hepatitis B through inhibiting hepatitis B virus. The anhydrous form of entecavir (ENT-A) often appeared as impurity polymorph in the manufacturing process of entecavir monohydrate (ENT-H) such as granulation, drying and compression. Since different crystal forms might affect drug bioavailability and therapeutic effect, it was vital to control the ENT-A content of the drug product. The work aim...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolomics and antioxidant activity of the leaves of Prunus dulcis Mill. (Italian cvs. Toritto and Avola)
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Alfredo Bottone, Paola Montoro, Milena Masullo, Cosimo Pizza, Sonia PiacenteAbstractPrunus dulcis leaves have been reported to exert some biological activity, in particular potent free radical-scavenging capacity, but so far there is limited information on their chemical composition. With the aim to achieve deep insight on the chemical constituents of the leaves of P. dulcis cultivars “Toritto” and “Avola”, the most appreciated in Italy, an approach based on liquid chromatography-mass sp...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Degradation caused by incompatibility between sodium stearyl fumarate (PRUV) and AZD7986 in the drug product
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Jufang Wu Ludvigsson, Håkan Wikström, Thomas Andersson, Per-Ola NorrbyAbstractDuring compatibility study of the AZD7986 project, a peak of 3 area% at the tail (RRT 1.03) of the active pharmaceutical ingredient (API) was discovered for all tablets containing sodium stearyl fumarate (PRUV) under humid condition (e.g. 50 °C/75% RH), regardless of choice of disintegrant or filler combination. The degradant was needed to be identified to understand the corresponding reaction mechanism and help the f...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Study of degradation behaviour of montelukast sodium and its marketed formulation in oxidative and accelerated test conditions and prediction of physicochemical and ADMET properties of its degradation products using ADMET Predictor™
This study was aimed to understand the degradation behaviour of the drug in different oxidative media containing hydrogen peroxide, AIBN, Fe3+, Fenton’s reagent and O2 environment under normal laboratory light conditions. The degradation behaviour of the drug was also evaluated in solid sate under ICH recommended accelerated stability condition of 40 °C/75% RH to correlate with the degradation products (DPs) formed in a solid oral formulation. A total of nine DPs (MTK 1 to MTK 9) were formed from both the drug substance and the marketed tablet formulation on storage under controlled oxygen environment in normal...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Identification and quantification of ethylene oxide in sterilized medical devices using multiple headspace GC/MS measurement
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Pascal Gimeno, Marie-Laure Auguste, Vagn Handlos, Anne Mette Nielsen, Stephan Schmidt, Nelly Lassu, Martin Vogel, Antonius Fischer, Charlotte Brenier, Françoise DuperrayAbstractThis manuscript, based on the ISO 10993-7 approach, describes a multiple HS-GC measurement of residual EO present in sterilized plastic samples. The quantification of EO is done, according to the ISO standard, by addition of EO amounts extracted for each repeated extraction. During the method development, the specificity of the de...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Multiwavelength UV-metric and pH-metric determination of the multiple dissociation constants of the lesinurad
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Milan Meloun, Aneta Čápová, Lucie Pilařová, Tomáš PekárekAbstractPotentiometric and spectrophotometric pH-titrations of the lesinurad for three consecutive dissociation constants determination were compared. Lesinurad is a selective inhibitor of uric acid reabsorption as part of a combination of medicines to treat high levels of uric acid in blood, also called hyperuricemia. Nonlinear regression of the pH-spectra with REACTLAB and SQUAD84 and of the pH-titration curve...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Study on the stability control strategy of Triphala solution based on the balance of physical stability and chemical stabilities
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Hao-zhou Huang, Sheng-yu Zhao, Xiu-mei Ke, Jun-zhi Lin, Shu-sen Huang, Run-chun Xu, Hong-yan Ma, Yi Zhang, Li Han, Ding-kun ZhangAbstractTriphala is a well-known prescription in Indian Ayurveda and TCM medicine for its great effect on gingivitis and hyperlipidemia. However, its solution is unstable for the containing of excessive polyphenol, leading to the production of sediment in the short term and the decrease of efficacy. Based on the analysis of sediment formation, a novel control strategy is proposed. To ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Comparison of multivariate data analysis techniques to improve glucose concentration prediction in mammalian cell cultivations by Raman spectroscopy
In this study, four mammalian cell cultivation runs were followed with Raman spectroscopy and the spectra were analysed quantitatively and qualitatively as well. The PCA analysis showed that one of the most dominant factors in the Raman spectra were the concentration of glucose, which strongly correlated with the score values of the eighth principal component. This observation further substantiated that Raman spectroscopy is an excellent tool for bioprocess monitoring and induced the test of the Multivariate Linear Regression (MLR), Principal Component Regression (PCR) and Partial Least Squares Regression (PLSR) algorithms...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of dextromepromazine and related substances 2-methoxyphenothiazine and levomepromazine sulfoxide in levomepromazine on a cellulose tris(4-methylbenzoate) chiral column
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Stephan Niedermeier, Iza Matarashvili, Bezhan Chankvetadze, Gerhard K.E. ScribaAbstractA high-performance liquid chromatography method for the determination of dextromepromazine, levomepromazine sulfoxide and 2-methoxyphenothiazine in levomepromazine samples was developed. The separation of the analytes was achieved within 10 min on a stationary phase containing cellulose tris(4-methylbenzoate) as chiral selector. The mobile phase consisted of 0.1% diethylamine in methanol with a flow rate of 1.0 mL/min. Th...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

A Quality by Design (QbD) approach to the development of a gradient high-performance liquid chromatography for the simultaneous assay of curcuminoids and doxorubicin from long-circulating liposomes
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Bianca Sylvester, Lucia Tefas, Laurian Vlase, Ioan Tomuţă, Alina PorfireAbstractThe present study highlights the advantages of using an Analytical Quality by Design (AQbD) approach to the optimization of a high-performance liquid chromatography method for the simultaneous assay of curcumin (CUR), demetoxycurcumin (DMC), bisdemetoxycurcumin (BDMC) and doxorubicin (DOX) co-encapsulated in long circulating liposomes. Within the QbD paradigm, the present study aimed to establish the method operable design region ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Chemometrics-assisted study of the interconversion between the crystalline forms of nimodipine
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Natalia L. Calvo, Naira M. Balzaretti, Marina Antonio, Teodoro S. Kaufman, Rubén M. MaggioAbstractNimodipine (NIM) is a calcium channel-blocking agent, which in the solid state exhibits two crystalline modifications, Mode I and Mode II. The first one is a racemic mixture, while the second is a conglomerate. Because the drug has poor aqueous solubility and Mode I is twice as soluble as Mode II, the former is widely preferred for the development of pharmaceutical forms. In order to study the effect of ther...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Acetylcholinesterase-inhibitory activity of Iranian plants: Combined HPLC/bioassay-guided fractionation, molecular networking and docking strategies for the dereplication of active compounds
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Mahdi Abbas-Mohammadi, Mahdi Moridi Farimani, Peyman Salehi, Samad Nejad Ebrahimi, Ali Sonboli, Celine Kelso, Danielle SkropetaAbstractIn order to search for discovery of acetylcholinesterase (AChE) inhibitors, as a therapeutic strategy for treatment of the Alzheimer’s disease, twenty-five Iranian plants have been evaluated by an in vitro enzymatic Ellman method and molecular docking study. Each plant was successively extracted by n-hexane, ethyl acetate and methanol to obtain a total of 75 extracts. The ...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of parecoxib and its main metabolites valdecoxib and hydroxylated valdecoxib in mouse plasma with a sensitive LC–MS/MS method to elucidate the decreased drug metabolism of tumor bearing mice
In this study, a sensitive, rapid and specific LC–MS/MS method for quantification of PX, VX and OH-VX in the plasma of tumor bearing mouse was developed and validated. After protein precipitation, all the analytes were separated on an Agilent ZORBAX Extend-C18 HPLC column (2.1 × 100 mm, 3.5 μm) with gradient elution. The analytes were detected by an electrospray negative ionization mass spectrometry in the multiple reaction monitoring mode. The transition m/z 369.0 → 119.0, m/z 312.9 → 117.9, m/z 329.0 → 196.0, and m/z 307.1 → 161.3 were used for monitoring PX, V...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Aromatic aldehydes as selective fluorogenic derivatizing agents for α‐dicarbonyl compounds. Application to HPLC analysis of some advanced glycation end products and oxidative stress biomarkers in human serum
Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Mahmoud H. El-Maghrabey, Taro Nakatani, Naoya Kishikawa, Naotaka KurodaAbstractα-Dicarbonyl compounds (α-DCs) are very clinically important as they are considered as advanced glycation end products (AGEs) precursors and biomarkers for many chronic diseases such as diabetes and vascular diseases, in addition to their major role in progression of complications of such diseases. Aromatic aldehydes and ammonium acetate were productively used as a one-pot co-reagents for fluorogenic derivatization of &al...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research