Bioanalytical liquid chromatography-tandem mass spectrometric assay for the quantification of the ALK inhibitors alectinib, brigatinib and lorlatinib in plasma and mouse tissue homogenates
Publication date: Available online 19 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rolf W. Sparidans, Wenlong Li, Alfred H. Schinkel, Jan H.M. Schellens, Jos H. BeijnenAbstractSeveral second and third generation ALK inhibitors have been introduced in recent years. A bioanalytical assay for simultaneous quantification of alectinib, brigatinib, and lorlatinib was developed and validated for human plasma. The method was also partially validated for diluted mouse plasma and tissue homogenates of brain, liver, kidney, and spleen. Samples (40 µl) were pretreated in a 96-well plate by pro...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Falsification of biotechnology drugs: Current dangers and/or future disasters?
Publication date: Available online 20 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Steven Janvier, Bart De Spiegeleer, Celine Vanhee, Eric Deconinck (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Use of thermal and non thermal techniques for assessing compatibility between mirtazapine and solid lipids
Publication date: Available online 20 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Randeep Kaur, V.R. SinhaAbstractThe present investigation was aimed at the evaluation of possible interactions between mirtazapine and selected solid lipids that are commonly used to develop solid lipid nanoparticles (SLNs) and nanostructured lipidic carriers (NLCs). The solids lipids explored were palmitic acid, stearic acid, glycerylmonostearate, cutina CPPH, sterotex NF, gelucire 50/13, hydrogenated castor oil and compritol 888 ATO. The techniques used were Differential Scanning Calorimetry (DSC), Fourier...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Nontargeted metabolomics approach to determine metabolites profile and antioxidant study of Tropical Almond (Terminalia catappa L.) fruit peels using GC-QTOF-MS and LC-QTOF-MS
The objective of the present study was to identify the metabolome pattern and study the biological efficacy of Almond (Terminalia catappa L.) peels. In the present study, metabolite profiling was carried out using GC-QTOF-MS and LC-QTOF-MS techniques, from the potent extract showed highest antioxidant efficacy. Antioxidant efficacy of the various extracts derived by six different extraction methods was assessed by different assays viz. 2,2-diphenyl-1-picrylhydrazyl, superoxide, 2,2′-Azino-bis-(3-ethyl)benzothiazoline)-6-sulfonic acid diammonium salt radical cation inhibition assays and ferric reducing antioxidant pow...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Novel electrophoretic acetonitrile-based stacking for sensitive monitoring of the antiepileptic drug perampanel in human serum
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Petr Tůma, Miroslava Bursová, Blanka Sommerová, Rachel Horsley, Radomír Čabala, Tomáš HložekAbstractPerampanel is a novel antiepileptic drug used in paediatric patients. Existing methods that determine serum perampanel are of limited practicability. We developed a novel capillary electrophoresis (CE) method using a new version of acetonitrile stacking for on-line sample pre-concentration, and fluorescence detection (FD). CE separations were performed in a fused-silica capill...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 17, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid authentication and differentiation of herbal medicine using 1H NMR fingerprints coupled with chemometrics
In conclusion, the developed method is an accurate, sensitive and rapid authentication of Polygoni Multiflori Radix, which could be a useful tool for rapid authentication and quality control of herbal medicines. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 14, 2018 Category: Drugs & Pharmacology Source Type: research

Paper-based analytical devices prepared with polycaprolactone printing and their application in the activity determination of mulberry extracts
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guo Shangxin, Shao Jingyuan, Gong XingchuAbstractA new method for preparing paper-based analytical devices (PADs) with the assistance of 3D printing technology was presented. Polycaprolactone was used as the 3D printing material to form hydrophobic barriers. First, polycaprolactone was 3D printed on filter paper. The polycaprolactone-modified paper was then prepared by heating. Experiments on α-glucosidase inhibition activity were carried out in the hydrophilic circles on the modified paper. Color data...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research

Recent advancements, challenges, and practical considerations in the mass spectrometry-based analytics of protein biotherapeutics: a viewpoint from the biosimilar industry
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Viktor Háda, Attila Bagdi, Zsolt Bihari, Sarolta Baginé Timári, Ádám Fizil, Csaba SzántayAbstractThe extensive analytical characterization of protein biotherapeutics, especially of biosimilars, is a critical part of the product development and registration. High-resolution mass spectrometry became the primary analytical tool used for the structural characterization of biotherapeutics. Its high instrumental sensitivity and methodological versatility made it possible t...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research

Untargeted rat brain metabolomics after oral administration of a single high dose of cannabidiol
Publication date: Available online 11 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cinzia Citti, Federica Palazzoli, Manuela Licata, Antonietta Vilella, Giuseppina Leo, Michele Zoli, Maria Angela Vandelli, Flavio Forni, Barbara Pacchetti, Giuseppe CannazzaAbstractCannabidiol (CBD), for long time considered as a minor cannabinoid of Cannabis sativa, has recently gained much attention due to its antioxidant, anti-inflammatory, analgesic and anticonvulsant properties. A liquid chromatography coupled to mass spectrometry based method was developed for the quantitative determination of CBD and ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 12, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of hepatotoxic and nephrotoxic potential markers of triptolide in mice with delayed-type hypersensitivity
In this study, a feasible strategy using HPLC-HRMS method combined with multivariate statistical analysis to discover toxic potential markers of TP was developed. TP was used to treat a DTH mouse model at a therapeutic dose (45μg/kg) and toxic dose (900 μg/kg). The metabolic profiles of the liver, kidney and plasma were characterized by HPLC-Q/TOF MS. Significant differences in the metabolite profiles of the liver, kidney and plasma existed between the toxic and therapeutically dosed mice. Forty-six metabolites were identified and 27 of them may be related to toxicity based on a structure-toxicity prediction model. U...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Kineret Protein Solution Survives Ten Years
Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yinghua Peng, Xiaohui WangAbstractProteins are labile and their shelf lives are usually months to few years. Herein we report that human interleukin-1 receptor antagonist (IL-1Ra) solution survives ten years. This provides an interesting case that protein biopharmaceutical aqueous solution could be stored for a decade without losing its quality, which could meet the national security needs for 10 years shelf life of biopharmaceuticals. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Novel controllable hydrophilic thermo-responsive molecularly imprinted resin adsorbent prepared in water for selective recognition of alkaloids by thermal-assisted dispersive solid phase extraction
Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Weiyang Tang, Kyung Ho RowAbstractA novel controllable hydrophilic thermo-responsive molecularly imprinted resin (T-MIR) with a switchable zipper-like architecture was synthesized in the aqueous phase and applied to the selective recognition and extraction of alkaloids by positive temperature regulation. In this synthesis process of T-MIR, 2-acrylamide-2- methylpropanesulfonic acid (AMPS) and acrylamide (AAm) were coupled as zipper-like thermo-responsive monomers, resorcinol, and melamine as hydrophilic mono...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of phytochemical components of Ferula halophila extracts using HPLC-MS/MS and their pharmacological potentials: A multi-functional insight
Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gokhan Zengin, Ahmet Uysal, Alina Diuzheva, Erdogan Gunes, József Jekő, Zoltán Cziáky, Carene Marie Nancy Picot-Allain, Mohamad Fawzi MahomoodallyAbstractThe inhibitory action of F. halophila extracts (acetone, chloroform, and methanol) against key enzymes linked to diabetes (α-amylase, α-glucosidase), cognitive enhancing functions (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE)), and hyperpigmentation (tyrosinase) was assessed. The mutagenic/antimutagenic activ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitation of residual host cell DNA in protaminesulfate drug product by qPCR
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Cynthia Sommers, Barry Rosenzweig, Lida Oum, Karol Thompson, David A. KeireAbstractProtamine sulfate (PS) is an FDA approved drug used to reverse heparin-induced anticoagulation in patients. Protamine sulfate is a mixture of primarily four ∼4 kDa arginine-rich cationic polypeptide chains derived from chum (Oncorhynchus keta) salmon sperm. Because the presence of residual host cell salmon DNA (resDNA) in PS drug product can pose safety concerns, processing steps during PS manufacturing are designed to ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 11, 2018 Category: Drugs & Pharmacology Source Type: research

Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells
This study demonstrated 77 protein features that were significantly changed in protein expression and revealed evodiamine-induced cytotoxicity in thyroid cancer cells involves dysregulation of protein folding, cytoskeleton, cytoskeleton regulation and transcription control. Our work shows that this combined proteomic strategy provides a rapid method to study the molecular mechanisms of evodiamine-induced cytotoxicity in thyroid cancer cells. The identified targets may be useful for further evaluation as potential targets in thyroid cancer therapy.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

Investigation of the thermal shift assay and its power to predict protein and virus stabilizing conditions
Publication date: Available online 9 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dora Sviben, Branimir Bertoša, Andrea Hloušek-Kasun, Dubravko Forcic, Beata Halassy, Marija BrglesAbstractProtein thermal shift assay (TSA) has been extensively used in investigation of protein stabilization (for protein biopharmaceutics stabilization, protein crystallization studies or screening of recombinant proteins) and drug discovery (screening of ligands or inhibitors). This work aimed to analyze thermal shift assay results in comparison to protein polymerization (multimerization and aggr...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

Saliva of patients affected by salivary gland tumour: An NMR metabolomics analysis
Publication date: Available online 9 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Manuela Grimaldi, Angelica Palisi, Giuseppina Rossi, Ilaria Stillitano, Francesco Faiella, Paola Montoro, Manuela Rodriquez, Remo Palladino, Anna Maria D’Ursi, Rocco RomanoAbstractCancers affecting the salivary glands have been an increasing incidence. Salivary gland cancer is not detected until it reaches an advanced stage, which would generally result in a poor prognosis and survival rate. Therefore, early detection as well as the screening of high risk populations with precancerous lesions remains an...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Combing metabolomics with bioanalysis methods to study the antitumor mechanism of the new acridone derivative 8q on CCRF-CEM cells: 8q induced mitochondrial-mediated apoptosis and targeted the PI3K/AKT/FOXO1 pathway
In conclusion, these findings demonstrate that 8q can induce mitochondrial lesions and promote mitochondrial-mediated pathway apoptosis by regulating the expression of Bcl-2 family proteins and inhibiting the activity of the PI3K/AKT/FOXO1 signaling pathway.Graphical abstractThe novel acridone derivative, 8q, can induce mitochondrial lesions and promote mitochondrial-mediated pathway apoptosis by regulating the expression of Bcl-2 family proteins and inhibiting the activity of the PI3K/AKT/FOXO1 signaling pathway to against CCRF-CEM cells. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Analytical aspects of sunitinib and its geometric isomerism towards therapeutic drug monitoring in clinical routine
Publication date: Available online 8 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bianca Posocco, Mauro Buzzo, Luciana Giodini, Sara Crotti, Sara D’Aronco, Pietro Traldi, Marco Agostini, Elena Marangon, Giuseppe ToffoliAbstractSunitinib malate, an oral multi-targeted tyrosine kinase inhibitor approved for the treatment of metastatic renal cell carcinoma, gastrointestinal stromal tumor, and well-differentiated pancreatic neuroendocrine tumors, has been identified as a potential candidate for therapeutic drug monitoring approach. Nevertheless, the development of an analytical assay sui...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

The application of on-line two-dimensional liquid chromatography (2DLC) in the chemical analysis of herbal medicines
Publication date: Available online 8 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shuai Ji, Shuang Wang, Haishan Xu, Zhenyu Su, Daoquan Tang, Xue Qiao, Min YeAbstractHerbal medicines are complicated chemical systems containing hundreds of small molecules of various polarities, structural types, and contents. Thus far, the chromatographic separation of herbal extracts is still a big challenge. Two-dimensional liquid chromatography (2DLC) has become an attractive separation tool in the past few years. Particularly, a lot of attention has been paid to on-line 2DLC. In this review, we aim to g...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Rapid authentication and differentiation of herbal medicine using 1H-NMR fingerprints coupled with chemometrics
In conclusion, the developed method is an accurate, sensitive and rapid authentication of Polygoni Multiflori Radix, which could be a useful tool for rapid authentication and quality control of herbal medicines. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Advancement in the chemical analysis of Paeoniae Radix (Shaoyao)
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Binjun Yan, Menglan Shen, Jinyang Fang, Danni Wei, Luping QinAbstractPaeoniae Radix Alba (baishao or white peony root) and Paeoniae Radix Rubra (chishao or red peony root) are two highly valuable traditional Chinese medicines (TCMs) usually indicated for painful conditions, menstrual disorders and viral infections. These two TCMs are collectively referred to as shaoyao (Paeoniae Radix) due to their close origins and similar chemical compositions. Modern research indicates that monoterpene glycosides, polyphen...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Novel electrophoretic acetonitrile-based stacking for sensitive monitoring of the novel antiepileptic drug perampanel in human serum
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Petr Tůma, Miroslava Bursová, Blanka Sommerová, Rachel Horsley, Radomír Čabala, Tomáš HložekAbstractPerampanel is a novel antiepileptic drug used in paediatric patients. Existing methods that determine serum perampanel are of limited practicability. We developed a novel capillary electrophoresis (CE) method using a new version of acetonitrile stacking for on-line sample pre-concentration, and fluorescence detection (FD). CE separations were performed in a fused-silica cap...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Silver Nanoparticles Decorated Polyaniline Nanocomposite Based Electrochemical Sensor for the Determination of Anticancer Drug 5-Fluorouracil
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Fatemeh Modarres Zahed, Behnaz Hatamluyi, Farnaz Lorestani, Zarrin Es’haghiAbstractA highly efficient electrochemical sensor for the analysis of anticancer drug 5-fluorouracil (5-FU), is fabricated based on silver nanoparticles-polyaniline nanotube (AgNPs@PANINTs). AgNPs@PANINTs nanocomposite has been synthesized by a simple one-step method. Synthesized AgNPs@PANINTs nanocomposite was studied by Fourier transform infrared spectrometry, Scanning Electron Microscopy and Energy Dispersive X-ray. The fabric...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Katrin N. Sander, Daniel P. Hay, Fiona Broughton-Pipkin, Raheela N. Khan, David A. BarrettAbstractThe analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect on the quality of quantitative results. Therefore, a range of mechanical homogenisation techniques and normalisation strategies were evaluated for application to huma...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Diagnostic Absolute Configuration Determination of Clavulanate Potassium: A Comprehensive Investigation of Chiroptical Spectroscopies and Theoretical Calculations
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhen Li, Linwei Li, Yi Zheng, Cong Chen, Tiemin SunAbstractClavulanate potassium (CP) is a classic β-lactamase inhibitor, which is extensively used as a combination drug in antibiotics therapy. As a chiral drug, CP is the only effective stereoisomer revealing prominent activities. To determine the chirality of CP efficiently, we provide an accurate and convenient method based on the comprehensive investigation of circular dichroism spectra and theoretical calculations. The results demonstrate two disting...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

LC-MS Analysis of Key Components of the Glutathione Cycle in Tissues and Body Fluids from Mice with Myocardial Infarction
Publication date: Available online 7 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Andres Gil, Atze van der Pol, Peter van der Meer, Rainer BischoffAbstractOxidative stress is suggested to play an important role in several pathophysiological conditions. A recent study showed that decreasing 5-oxoproline (pyroglutamate) concentration, an important mediator of oxidative stress, by over-expressing 5-oxoprolinase, improves cardiac function post-myocardial infarction in mice. The aim of the current study is to gain a better understanding of the role of the glutathione cycle in a mouse model of m...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 8, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of alkaloids dicentrine and sinomenine in Stephania epigeae by 1H NMR spectroscopy
Publication date: Available online 6 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jian-Wei Dong, Xue-Jiao Li, Le Cai, Jun-You Shi, Yu-Feng Li, Cui Yang, Zhen-Jie LiAbstractStephania epigaea Lo is an important herbal medicine used as antiphlogistic and analgesic drugs. Its major components are dicentrine (1) and sinomenine (2). In the present study, a rapid, accurate, and precise method for simultaneous quantitation of dicentrine (1) and sinomenine (2) in S. epigeae using 1H NMR spectra was developed. The deuterated solvent of DMSO-d6 enabled satisfactory separation of the signals to be int...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 7, 2018 Category: Drugs & Pharmacology Source Type: research

Diffusion-ordered spectroscopy on a benchtop spectrometer for drug analysis
Publication date: Available online 6 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gaëtan Assemat, Boris Gouilleux, Dylan Bouillaud, Jonathan Farjon, Véronique Gilard, Patrick Giraudeau, Myriam Malet-MartinoAbstractThe first reported two-dimensional diffusion-ordered spectroscopy (DOSY) experiments were recorded at low field (LF) on a benchtop NMR spectrometer using the BPP-STE-LED (bipolar pulse pair-stimulated echo sequence with a longitudinal eddy current delay) pulse sequence which limits phase anomalies and baseline discrepancies. A LF DOSY map was first obtained from a sol...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 7, 2018 Category: Drugs & Pharmacology Source Type: research

Recent developments in green membrane-based extraction techniques for pharmaceutical and biomedical analysis
Publication date: Available online 6 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hadi Tabani, Saeed Nojavan, Michal Alexovič, Ján SaboAbstractMonitoring of target analytes (e.g., pharmaceuticals, endogenous compounds) present in biological samples usually requires a preliminary step toward analyte isolation from surrounding matrix and enrichment for trace analysis. Evident developments have been recently made to introduce novel “green” analytical approaches (which keep the requirements of Green Analytical Chemistry – GAC) being effective, economical, eco-friendly...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 7, 2018 Category: Drugs & Pharmacology Source Type: research

Analytical methods for the study of bioactive compounds from medicinally used Echinacea species
Publication date: Available online 6 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Renato Bruni, Virginia Brighenti, Lindsay K. Caesar, Davide Bertelli, Nadja B. Cech, Federica PellatiAbstractEchinacea purpurea (L.) Moench, Echinacea angustifolia DC. var. angustifolia and Echinacea pallida (Nutt.) Nutt. are frequently used as medicinal plants and their preparations are among the most widely used herbal medicines. The extracts from these species have shown a highly complex chemical composition, including polar compounds (caffeic acid derivatives, CADs), non-polar compounds (alkylamides and a...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 7, 2018 Category: Drugs & Pharmacology Source Type: research

Study of the interaction of broad-spectrum antimicrobial drug sitafloxacin with human serum albumin using spectroscopic methods, molecular docking, and molecular dynamics simulation
This study are useful for further drug development, in vivo toxicity studies, and can provide guidance for the clinical application of STFX to study its pharmacokinetic properties.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - August 4, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous optimization of mobile phase composition and pH using retention modeling and experimental design
Publication date: Available online 2 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Norbert Rácz, Imre Molnár, Arnold Zöldhegyi, Hans-Jürgen Rieger, Róbert KormányAbstractChromatographic methods are progressing continuously. Increasing sample complexity and safety expectations lead to higher regulatory demands, hence challenges in liquid chromatography analysis are rising, even today, when faster and faster chromatographic systems are extensively employed and become widely accessible for successful method development.The goal of this study was to investi...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Development of a double-antibody sandwich ELISA for rapid detection to C-peptide in human urine
Publication date: Available online 2 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Rui Lv, Yingshan Chen, Nana Xia, Yuxin Liang, Qiyi He, Minyou Li, Zongxian Qi, Yeyu Lu, Suqing ZhaoAbstractC-peptide level is recognized as an important indicator of diabetes diagnosis. A sensitive and specific double-antibody sandwich enzyme-linked immunosorbent assay for the detection of C-peptide based on double antibody sandwich method was studied in this paper. The rabbit and hen were innunized with PLL-C-peptide and BSA-C-peptide respectively to obtain specific Yolk antibody (IgY) and polyclonal antibod...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Effects of Dibutyl phthalate and Di(2-ethylhexyl) phthalate with their metabolites on CYP2C9*1 and CYP2C19*1 activities in vitro
In this study, recombinant CYP2C9*1 and CYP2C19*1 microsomes were used to investigate the effects of phthalates and their metabolites on corresponding enzyme activities in vitro. 2-100 μM substrate of enzyme was incubated with series concentration of phthalates for 30 min at 37 °C. The metabolic products were detected using ultra-high performance liquid chromatography (UHPLC) and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) methods. The results showed dibutyl phthalate (DBP) significantly inhibited CYP2C9*1 with an activity inhibition rate of 67.3% and half-maxima...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level
Publication date: Available online 2 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julien Camperi, Audrey Combes, Jean Guibourdenche, Davy Guillarme, Valerie Pichon, Thierry Fournier, Nathalie DelaunayAbstractFor the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS). This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sit...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Multiresidue determination of 21 pharmaceuticals in crayfish (Procambarus Clarkii) using enzymatic microwave-assisted liquid extraction and ultrahigh-performance liquid chromatography - triple quadrupole mass spectrometry analysis
Publication date: Available online 2 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julia Kazakova, Rut Fernández-Torres, María Ramos-Payán, Miguel Ángel Bello-LópezAbstractIn this paper, a multiresidue enzymatic-microwave assisted extraction prior to ultrahigh performance liquid chromatography and triple quadrupole mass spectrometry analysis has been developed for the determination of 21 pharmaceuticals in crayfish (Procambarus Clarkii) samples. The analysed compounds corresponding to 6 therapeutic families were: fluoroquinolones (ciprofloxacin, danofloxac...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS) Assay for Simultaneous Quantifications of CZ48, Lactone-stabilized Camptothecin, and Camptothecin and Their Pharmacokinetic and Biliary Evaluations in Rats
In this study, a UHPLC-MS/MS method was developed and validated to simultaneously quantify CZ48 and CPT in rat plasma and bile. Detection was performed using the API 3200 Q Trap triple quadrupole mass spectrometer in a positive ion mode. Chromatographic separation was achieved on Waters ACQUITY UPLC BEH Shield RP18 column with a gradient elution at a flow rate of 0.45 ml/min, using mobile phases of 0.1% acetic acid in water (A) and 0.1% acetic acid in acetonitrile (B). The method was linear at the concentration ranges of 0.98 (LLOQ) – 1,000 ng/ml of CZ48 and CPT in rat plasma and 3.9 (LLOQ) – 1,00...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Simultaneous quantification of cannabinoids tetrahydrocannabinol, cannabidiol and CB1 receptor antagonist in rat plasma: An application to characterize pharmacokinetics after passive cannabis smoke inhalation and co-administration of rimonabant
Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Abhigyan Ravula, Hardik Chandasana, Barry Setlow, Marcelo Febo, Adriaan W. Bruijnzeel, Hartmut DerendorfAbstractA highly sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of tetrahydrocannabinol (THC), cannabidiol, and rimonabant in rat plasma was developed. Analytes and the internal standard were extracted from plasma using a combination of protein precipitation followed by liquid-liquid extraction. Chromatographic separation was done using Waters Symmetry C18, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Immobilized enzyme-based analytical tools in the -omics era: recent advances
Publication date: Available online 31 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marina Naldi, Anna Tramarin, Manuela BartoliniAbstractProtein analysis is a field under rapid development mainly thanks to technological advances which have granted miniaturization of analytical devices, automation and higher detection sensitivity. The interest in the field has paralleled the expansion of the -omics era, laying down the bases for the current proteomic and glycomic applications. Advances in protein sample transformation prior to analysis have led to limitation in sample consumption and contamin...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 1, 2018 Category: Drugs & Pharmacology Source Type: research

Phytochemical profile of Euphorbia peplus L. collected in Central Italy and NMR semi-quantitative analysis of the diterpenoid fraction
Publication date: Available online 31 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Claudio Frezza, Alessandro Venditti, Fabio Sciubba, Pierpaolo Tomai, Marika Antonetti, Marco Franceschin, Maria Enrica Di Cocco, Alessandra Gentili, Maurizio Delfini, Mauro Serafini, Armandodoriano BiancoAbstractIn this work, the phytochemical profile of the ethanolic extract of Euphorbia peplus L. collected in Central Italy, was reported. This specimen had never been studied before and the analysis was accomplished by means of Column Chromatography for the separation procedure and by means of NMR Spectroscopy...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 1, 2018 Category: Drugs & Pharmacology Source Type: research

Volatile component interaction effects on compatibility of Cyperi Rhizoma and Angelicae Sinensis Radix or Chuanxiong Rhizoma by UPLC-MS/MS and response surface analysis
Publication date: Available online 31 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Pei Liu, Er-xin Shang, Yue Zhu, Da-wei Qian, Jin-ao DuanABSTRACTCyperi Rhizoma (Xiangfu) combined with either Angelicae Sinensis Radix (Danggui) or Chuanxiong Rhizoma (Chuanxiong) are herb pairs that are commonly used in clinical settings. To illustrate the herb–herb interactions that occur when extracts from these herbs are obtained together, ultra-high-performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry and response surface analysis were used. Volatile...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 1, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitation of Residual Host Cell DNA in Protamine Sulfate Drug Product by qPCR Assay
Publication date: Available online 31 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cynthia Sommers, Barry Rosenzweig, Lida Oum, Karol Thompson, David A. KeireAbstractProtamine sulfate (PS) is an FDA approved drug used to reverse heparin-induced anticoagulation in patients. Protamine sulfate is a mixture of primarily four ~4 kDa arginine-rich cationic polypeptide chains derived from chum (Oncorhynchus keta) salmon sperm. Because the presence of residual host cell salmon DNA (resDNA) in PS drug product can pose safety concerns, processing steps during PS manufacturing are designed to ta...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 31, 2018 Category: Drugs & Pharmacology Source Type: research

Risk-based approach for method development in pharmaceutical quality control context: A critical review
Publication date: Available online 30 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Riccardo Deidda, Serena Orlandini, Philippe Hubert, Cédric HubertAbstractPharmaceutical regulatory bodies increasingly require the implementation of systematic approaches in pharmaceutical product development. Quality control methods play a key role in the control strategy of drugs manufacturing to assure their quality. A risk-based approach in the analytical method development is strongly recommended to ensure that the method performances fit the purpose of the method during its entire life-cycle. In t...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 31, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative Analysis of a Pharmaceutical Formulation: Performance Comparison of Different Handheld Near-Infrared Spectrometers
Publication date: Available online 30 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hui Yan, Heinz W. SieslerAbstractNotwithstanding the first developments of miniaturized vibrational spectrometers more than a decade ago, only recently real handheld near-infrared (NIR) spectrometers (
Source: Journal of Pharmaceutical and Biomedical Analysis - July 31, 2018 Category: Drugs & Pharmacology Source Type: research

Investigating the utility of minimized sample preparation and high-resolution mass spectrometry for quantification of monoclonal antibody drugs
In this study, we investigate the capabilities of high-resolution MS instruments for circumventing the complex sample preparation currently needed for sensitive LC–MS/MS-based quantification of mAbs. Employing a simple one-step sample pre-treatment workflow, we compare the ability of three different LC–MS platforms for absolute quantification of a representative monoclonal antibody Rendomab-B1 in serum and plasma. The samples are subjected to protein precipitation with methanol, followed by pellet digestion with trypsin prior to LC–MS analysis. AQUA peptides based on two surrogate mAb peptides selected fr...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 31, 2018 Category: Drugs & Pharmacology Source Type: research

UHPLC-MS/MS method for determination of atorvastatin calcium in human plasma: Application to a pharmacokinetic study based on healthy volunteers with specific genotype
Publication date: Available online 30 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Binbin Xia, Yali Li, Yatong Zhang, Ming Xue, Xiaorong Li, Pingxiang Xu, Tao Xia, Shicai ChenAbstractA rapid, selective and sensitive ultra high performance liquid chromatography coupled with tandem triple quaternary mass spectrometry (UHPLC-MS/MS) method was developed and validated for the quantitative determination of atorvastatin calcium (AC) in human plasma. Separation of AC and rosuvastatin calcium (internal standard, IS) were achieved on a Dikma Leapsil C18 reversed phase column (100 ×&thinsp...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 30, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a rapid ultra-high performance liquid chromatography diode array detector method for Verbena officinalis L
This study presents a fast and validated ultra-high performance liquid chromatography diode array detector (UHPLC-DAD) method for the simultaneous determination of the major compounds in Verbena officinalis L. (Verbenaceae), a medicinal plant listed in the European, British, and, Chinese Pharmacopoeias. In order to get reference substances, nine compounds, belonging to iridoids, flavonoids, and phenylpropanoid glycosides, were isolated from the herb extract. Two of them, namely cistanoside D and leucosceptoside A, were found in this plant species for the first time. Chromatographic separation was achieved in less than 7&th...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 28, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of polydisperse macrogols and macrogol-based excipients via HPLC and charged aerosol detection
Publication date: Available online 27 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Christiane Theiss, Ulrike HolzgrabeAbstractMacrogol-based emulsifiers and their respective precursor substances, i.e. macrogols (PEG), fatty acids (FA), and fatty alcohols (FAA), are widely used excipients which are usually characterized by a series of tests described within the European Pharmacopoeia (Ph. Eur.). Examples are bulk parameters such as the hydroxyl value, the peroxide value, and the determination of fatty acids composition by gas chromatography. The choice of tests depends on the emulsifier consi...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 28, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolite profiling of “green” extracts of Corylus avellana leaves by 1H NMR spectroscopy and multivariate statistical analysis
Publication date: Available online 26 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Antonietta Cerulli, Milena Masullo, Paola Montoro, Jan Hošek, Cosimo Pizza, Sonia PiacenteAbstractCorylus avellana L. (Betulaceae) leaves, consumed as infusion, are used in traditional medicine, for the treatment of hemorrhoids, varicose veins, phlebitis, and edema due to their astringent, vasoprotective, and antiedema properties. In previous works we reported from the leaves of Corylus avellana cv. “Tonda di Giffoni” diarylheptanoid derivatives, a class of plant secondary metabolites with a...
Source: Journal of Pharmaceutical and Biomedical Analysis - July 27, 2018 Category: Drugs & Pharmacology Source Type: research