Rapid discrimination between red and white ginseng based on unique mass-spectrometric features
In this study, some of these unique features were identified and confirmed as biomarkers of RG by using ultra-high-performance liquid chromatography-mass spectrometry, data mining, support vector machine, and artificial neural network. Principal component analysis showed clear separation between the RG and WG extracts, indicating the presence of potential discriminators. In addition, 20 features that are dominant in RG were found by data mining. Samples of Panax quinquefolium (PQ) and Panax notoginseng (PN), close relatives of Panax ginseng C.A.Meyer, were investigated and it was found that 17 features which were absent in...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Ultrasensitive and reusable electrochemical aptasensor for detection of tryptophan using of [Fe(bpy)3](p-CH3C6H4SO2)2 as an electroactive indicator
Publication date: Available online 3 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ayemeh Bagheri Hashkavayi, Jahan Bakhsh RaoofAbstractIn this paper, we report the application of a reusable electrochemical aptasensor for detection of tryptophan by using [Fe(bpy)3](p-CH3C6H4SO2)2 as an electroactive indicator and based on the target-compelled aptamer displacement. The aptasensor fabricated by self-assembling the thiolated probe on the surface of graphite screen-printed electrode modified with gold nanoparticles/multiwalled carbon nanotubes and chitosan nanocomposite (AuNPs/MWCNTs-Chit/SPE)...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Development, validation, and clinical application of a high-performance liquid chromatography-tandem mass spectrometry assay for the quantification of total intracellular β-decitabine nucleotides and genomic DNA incorporated β-decitabine and 5-methyl-2’-deoxycytidine
Publication date: Available online 3 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jeroen Roosendaal, Hilde Rosing, Luc Lucas, Aram Oganesian, Jan H.M. Schellens, Jos H. BeijnenAbstractDNA hypermethylation is an epigenetic event that is commonly found in malignant cells and is used as a therapeutic target for β-decitabine (β-DEC) containing hypomethylating agents (eg Dacogen® and guadecitabine). β-DEC requires cellular uptake and intracellular metabolic activation to β-DEC triphosphate before it can get incorporated into the DNA. Once incorporated in the DNA, β...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

The determination of gemini surfactants used as gene delivery agents in cellular matrix using validated tandem mass spectrometric method
Publication date: Available online 3 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jin Wei, Badea Ildiko, C. Leary Scot, El-need AnasAbstractA simple, reliable flow injection analysis (FIA)-tandem mass spectrometric (MS/MS) method was developed for the determination of gemini surfactants, designated as 16-3-16, 16(Py)-S-2-S-(Py)16 and 16-7 N(GK)-16, as gene delivery agents in cellular matrix. 16-3-16 is a conventional gemini surfactant bearing two quaternary amines, linked by a 3-carbon spacer region, 16(Py)-S-2-S-(Py)16 contains two pyridinium head groups, while 16-7 N(GK)-1...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

A UHPLC-MS/MS method coupled with liquid-liquid extraction for the quantitation of phenacetin, omeprazole, metoprolol, midazolam and their metabolites in rat plasma and its application to the study of four CYP450 activities
In this study, we established a UHPLC-MS/MS method for simultaneous quantitation of four CYP450 probe drugs (phenacetin, omeprazole, metoprolol and midazolam) and their metabolites (acetaminophen, 5'-hydroxy omeprazole, α-hydroxy metoprolol and 1'-hydroxy midazolam) in rat plasma. Sample preparation by plasma protein precipitation was combined with a liquid-liquid extraction method. The separation was carried out on a ZORBAX Eclipse Plus C18 Rapid Resolution High Definition column with a gradient elution, using water containing 0.1% formic acid (A) and acetonitrile (B) in a run time of only 3.0 min. Detection ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of Velvet Antler and Its Mixed Varieties by UPLC-QTOF-MS Combined with Principal Component Analysis
Publication date: Available online 4 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiao-han Guo, Xian-Long Cheng, Wen-Xi Liu, Ming-Hua Li, Feng Wei, Shuang-Cheng MaAbstractMany species of velvet antler have been used as traditional medicine for thousands of years; however, as medicinal materials, velvet antler derived from different animals have different clinical effects. To distinguish the differences and homologies, ultra-performance liquid chromatography-quadruple-time of flight mass spectrometry (UPLC-QTOF-MS) coupled with principal component analysis (PCA) was developed and applied t...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

UPLC-MS/MS determination of suvorexant in urine by a simplified dispersive liquid-liquid micro-extraction followed by ultrasound assisted back extraction from solidified floating organic droplets
In this study, a highly sensitive UPLC-MS/MS assay was developed and validated for the determination of suvorexant in urine sample. A simplified dispersive liquid-liquid microextraction followed by ultrasound assisted back extraction from solidified floating organic droplets was employed for sample preparation. The 20 µL of 1-undecanol and 200 µL of acetonitrile were used as extraction solvent and dispersive solvent, respectively. An ultrasound assisted back extraction step was employed to enable the cleanup procedure compatible with mass spectrometric detection. Acquity CSHTM C18 column with mobi...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of caffeoylquinic acid derivatives in Azolla imbricata by chitosan-based matrix solid-phase dispersion coupled with HPLC–PDA
Publication date: Available online 4 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Weilin Wu, Wenqi Qian, Haitian Hao, Yun kang, Yaqin Wang, Yuanrong Deng, Chengjie Ni, Jianming Huang, Weiyu WengAbstractFive main components of Azolla imbricata were isolated and identified as caffeoylquinic acid derivatives, four of which were isolated from this species for the first time. The five compounds exhibited strong antioxidant activities and were used as chemical markers for quantitative analysis. A chitosan-based matrix solid-phase dispersion (MSPD) extraction coupled with high-performance liquid...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Screening methods for rapid determination of new psychoactive substances (NPS) in conventional and non-conventional biological matrices
Publication date: Available online 4 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Silvia Graziano, Luca Anzillotti, Giulio Mannocchi, Simona Pichini, Francesco Paolo BusardòAbstractIn the last years, a global awareness has arisen from the reported harmful effects and public health risks associated with the consumption of new psychoactive substances (NPSs). Improving efforts in the detection and identification of these substances have emerged as a global analytical challenge involving the large range of NPSs’ chemical structures and the variety of conventional and non-conventi...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Chemical Comparison of Prunus africana Bark and Pygeum Products Marketed for Prostate Health
Publication date: Available online 5 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Robert Q. Thompson, Daniel Katz, Brendan SheehanAbstractThe bark of Prunus africana may contain atranorin, atraric acid, beta-sitosterol and its esters, ferulic acid and its esters, and N-butylbenzene sulfonamide, compounds that have been shown to improve the conditions of benign prostatic hyperplasia, enlarged prostate. An analytical scheme, involving liquid-solid extractions, saponifications, and LC-APCI-MS (triple quadrupole) analysis, was developed, optimized, and validated to determine the compounds at ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Is the hematocrit still an issue in quantitative dried blood spot analysis?
Publication date: Available online 5 October 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sofie Velghe, Lisa Delahaye, Christophe P. StoveAbstractHematocrit-related issues remain a major barrier for (regulatory) acceptance of the classical dried blood spot (DBS) analysis in the bioanalytical and clinical field. Lately, many attempts to cope with these issues have been made. Throughout this review, an overview is provided on new strategies that try to cope with this hematocrit effect (going from avoiding to minimizing), on methods estimating a DBS volume, and on methods estimating or measuring the...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Single vial cold kits optimized for preparation of gastrin releasing peptide receptor (GRPR)-radioantagonist 68Ga-RM2 using three different 68Ge/68Ga generators
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Kusum Vats, Rohit Sharma, Mythili Kameswaran, Drishty Satpati, Ashutosh DashAbstract68Ga-RM2 is a gastrin releasing peptide receptor (GRPR) antagonist PET (positron emission tomography) radiotracer which is being investigated in clinical trials as a potential prostate cancer imaging agent. Simple, one-step kit formulation of 68Ga-RM2 would facilitate multicentre trials and allow easier integration in hospital radiopharmacy. Herein we report development of three sets of single-vial RM2 cold kits validated for for...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Ionic liquid-based salt-induced liquid-liquid extraction of polyphenols and anthraquinones in Polygonum cuspidatum
In this study, ionic liquid was used as extraction solvent. The dried P. cuspidatum samples purchased from the pharmacy were triturated and passed through a 120-mesh sieve. The obtained sample powders were dried to constant weight at 55 ℃, and then mixed with extraction solvent. The extraction was carried out with the aid of ultrasound. Three phases, including ionic liquid-rich, salt-rich and solid sample phases were formed in the presence of salt. The target analytes were enriched in ionic liquid phase and then determined by high performance liquid chromatography. The experimental parameters, such as type and volume of ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Interactions of pharmacokinetic profiles of Ginkgotoxin and Ginkgolic acids in rat plasma after oral administration
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Yiyun Qian, Shulan Su, Min Wei, Zhenhua Zhu, Sheng Guo, Hui Yan, Jinhua Tao, Dawei Qian, Jin-ao DuanAbstractGinkgolic acids (GAs) and Ginkgotoxin (4′-O-methylpyridoxine, MPN) are main toxic compounds in Ginkgo biloba seeds which are widely used in the treatment of coughing in China. To evaluate the pharmacokinetics of GAs, MPN and their metabolites in rat plasma, a highly sensitive method followed by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (U...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of total and free ceftolozane and tazobactam in human plasma and interstitial fluid by HPLC-UV
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Alexander Kratzer, Selina Schießer, Peter Matzneller, Beatrix Wulkersdorfer, Markus Zeitlinger, Jens Schlossmann, Frieder Kees, Christoph DornAbstractCeftolozane/tazobactam is a new cephalosporin/beta-lactamase inhibitor combination. An HPLC-UV method is described for the determination of total and free ceftolozane and tazobactam in human plasma and in microdialysate of subcutaneous tissue, respectively. Separation was performed using a reversed-phase column with phosphate buffer/acetonitrile as eluent and...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics, tissue distribution and plasma protein binding study of SM-1, a novel PAC-1 derivative
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Qin Yi, Xuhua Han, Zhihong Fan, Yuehui Ma, Gangzhi Zhu, Wei Qiang, Lei Wang, Zeneng ChengAbstractAs a PAC-1 derivative, SM-1 exhibts a promising antitumour property. To better understand the relationship between the drug concentrations and pharmacological effects, both liquid chromatography coupled with tandem mass spectrometry and high performance liquid chromatography methods were developed and validated in the work. Those methods were then applied to the pharmacokinetics (PK), tissue distribution and plasma p...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

New insights into phenol and polyphenol composition of Stevia rebaudiana leaves
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Severina Pacifico, Simona Piccolella, Paola Nocera, Elisabetta Tranquillo, Francesco Dal Poggetto, Michelina CatauroAbstractThe diversity in phenols and polyphenols of stevia leaf has been simplified applying sequential fractionation techniques on its ethanol extract through ultrasound assisted maceration. Two of the fractions obtained by reverse-phase column chromatography resulted differently active in an extensive antioxidant and cytotoxic screening. Both fractions were chemically profiled by ultra-performanc...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

1H NMR-based dynamic metabolomics delineates the therapeutic effects of Baoyuan decoction on isoproterenol-induced cardiac hypertrophy
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Zhiyong Du, Ran Wen, Qian Liu, Jinlong Wang, Yingyuan Lu, Mingbo Zhao, Xiaoyu Guo, Pengfei Tu, Yong JiangAbstractCardiac hypertrophy (CH) is a major risk factor for many serious heart diseases. Sustained CH is catastrophic, resulting in cardiac dysfunction that eventually leads to heart failure (HF). Baoyuan decoction (BYD) is a famous traditional Chinese medicine (TCM) formula for supplementing and reinforcing Qi, clinically used for the treatment of cardiovascular diseases (CVDs). However, the therapeutic effe...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Metabolomic study of serum, urine and bronchoalveolar lavage fluid based on gas chromatography mass spectrometry to delve into the pathology of lung cancer
This study explores for the first time the combination of serum, urine and bronchoalveolar lavage fluid (BALF) to deep insight into the pathology of lung cancer (LC) using a metabolomic platform based on gas chromatography mass spectrometry (GCMS). The study includes LC patients, healthy control group (HC) and a group of patients with noncancerous lung diseases (NCC) used as a control group respect to BALF because of the invasive nature this fluid collection.The metabolomic platform was applied to serum, urine and BALF samples in order to compare the metabolomic profiles of these biological fluids and establish metabolic s...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

Peak window correlation method for drug screening using Raman spectroscopy
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Roman R. Galeev, Dmitry A. Semanov, Ekaterina V. Galeeva, Tatyana S. Falaleeva, Ilshat R. Aryslanov, Anatoly A. Saveliev, Rustam R. DavletshinAbstractModern portable and hand-held Raman spectrometers that recently have become widespread in drug quality screening have good reproducibility and are able to detect small concentrations of substances in mixtures of several components or distinguish compounds similar in structure and having minimal differences in spectrum with appropriate mathematical processing method...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2018 Category: Drugs & Pharmacology Source Type: research

A multidimensional analytical approach based on time-decoupled online comprehensive two-dimensional liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry for the analysis of ginsenosides from white and red ginsengs
Publication date: Available online 21 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hong Zhang, Jia-Ming Jiang, Dan Zheng, Ming Yuan, Zhi-Ying Wang, Hong-Mei Zhang, Chang-Wu Zheng, Lian-Bo Xiao, Hong-Xi XuAbstractHere, time-decoupled comprehensive two-dimensional ultra-high liquid chromatography (UHPLC) coupled with an ion mobility (IM)-high resolution mass spectrometer (HRMS) was established and used to analyze ginsenosides from the main roots of white ginseng (WG) and red ginseng (RG), which enabled the separation of complex samples in four dimensions (2D-LC, ion mobility, and mass spe...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2018 Category: Drugs & Pharmacology Source Type: research

Capillary Electrophoresis Method for the Determination of (R)-Dapoxetine, (3S)-3-(Dimethylamino)-3-phenyl-1-propanol, (S)-3-Amino-3-phenyl-1-propanol and 1-Naphthol as Impurities of Dapoxetine Hydrochloride
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Henrik Harnisch, Gerhard K.E. ScribaAbstractA capillary electrophoresis method was developed and validated for the determination of the purity of dapoxetine with regard to the related substances (3S)-3-amino-3-phenylpropan-1-ol, (3S)-3-(dimethylamino)-3-phenylpropan-1-ol, 1-naphthol and the enantiomer (R)-dapoxetine. The separation was based on a dual selector system, which was optimized by a fractional factorial resolution V + design followed by a central composite face centered design with...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Seeking Universal Detectors for Analytical Characterizations
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kelly Zhang, Kenji Kurita, Cadapakam Venkatramani, David RussellAbstractIt is highly desirable to have a universal detector that can detect all types of compounds and give a uniform response regardless of the physiochemical properties of the compounds. With such a universal detector, all components in a sample can be accurately quantified without the need for individual standards. This is especially needed for the characterization of unknowns and for non-targeted analysis, or for samples that have no isol...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Lipid profile perturbations in the plasma and lungs of mice with LPS-induced acute lung injury revealed by UHPLC-ESI-Q Exactive HF MS analysis
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jinjun Shan, Wenjuan Qian, An Kang, Linxiu Peng, Tong Xie, Lili Lin, Liuqing Di, Pingxi Xiao, Wei ZhouAbstractAn UHPLC-ESI-Q Exactive HF MS-based lipidomics method was successfully applied to profile various lipids from the plasma and lungs of mice intranasally challenged with lipopolysaccaride (LPS). Response trends of lipids to LPS were graphically represented by variable importance in projection (VIP) plot, heat map, and bar plot. As a result, 77 differential lipids in the lung and 13 differential lipi...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

An enzyme-modified capillary as a platform for simultaneous fluorometric detection of D-glucose and L- lactate
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Atsushi Shoji, Yusuke Takahashi, Saki Osato, Masao SugawaraAbstractThe preparation of a glass capillary pattered with lipid layers on which lactate dehydrogenase (LDH) and glucose dehydrogenase (GDH) were regionally adsorbed and its application for simultaneous detection of D-glucose and L-lactate in human serum is described. A lipid layer was formed on the surface of BSA-unabsorbed octadecyltrichlorosilane (OTS) inner wall of a glass capillary. The electrostatic charge of the lipid layer was a key fact...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Computational Design as a Green Approach for Facile Preparation of Molecularly Imprinted Polyarginine-Sodium Alginate-Multiwalled Carbon Nanotubes Composite Film on Glassy Carbon Electrode for Theophylline Sensing
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Maryam Salajegheh, Mehdi Ansari, Mohammad Mehdi Foroghi, Maryam KazemipourAbstractThis paper reports on the synthesis of a novel molecularly imprinted composite film using the mathematical modeling. This composite was then used in the electrode modification for the determination of theophylline. The ratio of monomer to template in optimum condition was obtained to be 4. The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composit...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Isotope Dilution LC-ESI-MS/MS and low resolution Selected Reaction Monitoring as a tool for the accurate quantification of urinary testosterone
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Amaia Ereño Artabe, Adriana González-Gago, Amanda Suarez Fernández, Jorge Pitarch Motellón, Antoni F. Roig-Navarro, Oscar J. Pozo, Pablo Rodríguez-González, J. Ignacio García AlonsoAbstractA new analytical method for the quantification of testosterone in human urine samples by isotope dilution mass spectrometry is proposed. A standard solution of 13C2-testosterone is added to the samples at the beginning of the sample preparation procedure and then the meas...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of inorganic anions in the whole blood by ion chromatography
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaohui He, Yuejing Mei, Yuchen Wang, Wenjun Sun, Mei ShenAbstractA fast, precise, and accurate method that can simultaneously determine 7 anions in whole blood was established by on line dialysis-double suppression ion chromatography. Performance parameters which could affect the determination of anions were optimized, including the selection of protein precipitant in samples, the amount of filtrate discarded, selection of eluent flow rate, influence of the Ag-Na column on experimental results, influence...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a method using ultra performance liquid chromatography coupled to tandem mass spectrometry for determination of zoledronic acid concentration in human bone
This study fully described the analytical process for the determination of ZA in human bone sequestrations, representing a pivotal step for further biomedical research on ZA and BRONJ. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

A new MS compatible HPLC-UV method for Teicoplanin drug substance and related impurities, part 1: development and validation studies
This study, which is the first in a series of two parts, describes the development of a LC method for the separation of Teicoplanin drug substance and its related impurities compatible with MS detection.The separation conditions for Teicoplanin were set on a LiChrospher 100 RP-18 column under gradient elution with a mobile phase composed of ammonium formate 25 mM at pH 6.00 and ACN. The new method was shown equivalent in terms of selectivity to the one reported in the European Pharmacopoeia Teicoplanin monograph, and was validated according to ICH Q2 R1 guidelines for the drug substance assay. The new method offers simil...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

NMR-based metabonomic approach reveals changes in the urinary and fecal metabolome caused by resveratrol
Publication date: Available online 20 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gabriela Torres Santiago, José Iván Serrano Contreras, María Estela Meléndez Camargo, L. Gerardo Zepeda VallejoAbstractAn untargeted NMR-based metabonomics approach was used to evaluate the effects of pure resveratrol (RSV, 50 and 250 mg/kg per os) on the urinary and faecal metabolome of normal female Wistar rats. Multivariate data analysis on both the endogenous and xenobiotic metabotype of RSV provided an insight into its metabolic fate and influence on endogenous meta...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Precision of the reportable value—Statistical optimization of the number of replicates
Publication date: 5 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 162Author(s): Richard K. Burdick, Joachim ErmerAbstractIn pharmaceutical analysis, the precision of the reportable value, i.e. the result which is to be compared to the specification limit(s), is relevant for the suitability of the analytical procedure. Using the variance contributions determined in precision studies addressing the levels injection/system precision, repeatability, and intermediate precision, the number of the corresponding replications for analysis/injection, sample preparation, and series/runs can be varied t...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Extensive Characterization and Differential Analysis of Endogenous Peptides from Bombyx Batryticatus Using Mass Spectrometric Approach
This study illustrates the abundance and sequences of endogenous Bombyx batryticatus polypeptides, and thus, provides potential candidates for the screening of active compounds for future biological research and drug discovery studies. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: in vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles
Publication date: Available online 18 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gokhan Zengin, Azzurra Stefanucci, Maria João Rodrigues, Adriano Mollica, Luisa Custodio, Muhammad Zakariyyah Aumeeruddy, Mohamad Fawzi MahomoodallyAbstractThe genus Scrophularia has received much interest with regards to its traditional usages such as eczema, psoriasis, and mastitis. Yet, the medicinal properties of some species still need to be scientifically validated. The present study was designed to investigate into the biological properties of various solvent extracts (ethyl acetate, methano...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Elucidation of pharmacokinetics of novel DNA ligase I inhibitor, S012-1332 in rats: Integration of in vitro and in vivo findings
Publication date: Available online 18 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Riyazuddin Mohammed, R. Valicherla Guru, Husain Athar, Kamil Hussain Mohd, Shukla Minakshi, Katekar Roshan, P. Gupta Anand, Singh Pragati, Banerjee Dibyendu, Hajela Kanchan, R. Gayen JiaurAbstractS012-1332 is the first DNA ligase I inhibitor that demonstrated in vivo anti-breast cancer activity. The present study aimed to assess the in vivo pharmacokinetics of S012-1332 in rats and interpret them with in vitro findings. A sensitive and selective liquid chromatography-tandem mass spectrometry bioanalytical...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Development of new methods for determination of bilirubin
Publication date: Available online 18 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lightson Ngashangva, Vinay Bachu, Pranab GoswamiAbstractThe ever-increasing demand for a sensitive, rapid and reliable method for determination of serum bilirubin level has been inciting the interest of the researchers to develop new methods for both laboratory set up and point of care applications. These efforts embrace measurement of different forms of bilirubin, such as, unconjugated (free and albumin bound) bilirubin, conjugated (direct) bilirubin, and total (both conjugated and unconjugated) bilirubi...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of the in vitro metabolites of idelalisib in liver microsomes and interspecies comparison
Publication date: Available online 15 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cai-Ming Chen, Wei-Bo Wu, Jian-Feng Chen, Yan ChenAbstractIdelalisib acts as a phosphoinositide 3 kinase inhibitor, which has been approved by the US FDA for the treatment of certain hematological malignancies. The aim of this study is to profile the metabolites of idelalisib in the liver microsomes of mouse, rat, rabbit, dog, monkey and human. Idelalisib at the concentration of 20 μM was incubated with the liver microsomes in the presence of NADPH, GSH and UDPGA. The incubation samples were ana...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 16, 2018 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics and toxicokinetics of D-serine in rats
Publication date: Available online 14 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hiroshi Hasegawa, Nami Masuda, Hiromi Natori, Yoshihiko Shinohara, Kimiyoshi IchidaAbstractIn the mammalian brain, D-serine acts as a co-agonist at the glycine-binding site on the N-methyl-D-aspartate receptor. Because plasma D-serine levels are significantly lower in patients with schizophrenia than in healthy subjects, D-serine has been proposed as a potential therapeutic agent for schizophrenia treatment. However, D-serine has a nephrotoxic effect in rats at high doses. The purpose of this study was to...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 15, 2018 Category: Drugs & Pharmacology Source Type: research

Process analytical technology for continuous manufacturing tableting processing: A case study
Publication date: Available online 13 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Victoria Pauli, Yves Roggo, Laurent Pellegatti, Nhat Quang Nguyen Trung, Frantz Elbaz, Simon Ensslin, Peter Kleinebudde, Markus KrummeAbstractThe use of Near Infrared Spectroscopy (NIRS) as a fast and non-destructive technique was employed for the control and monitoring of the tableting step during a continuous manufacturing process.Two NIRS methods were optimized in order to in-line control the blend uniformity in the tablet feed frame and the API concentration of freshly pressed tablets prior the ejecti...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2018 Category: Drugs & Pharmacology Source Type: research

Comparison of in vitro/in vivo blood distribution and pharmacokinetics of artemisinin, artemether and dihydroartemisinin in rats
Publication date: Available online 13 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tianming Dai, Weifan Jiang, Zizheng Guo, Yanxiang Xie, Renke DaiAbstractArtemisinin and its derivatives have been widely used for treatment of malaria and the therapeutic targets are considered within the red blood cells. In the recent studies on the erythrocytes’ uptake of artemisinin-derivatives in vitro, applying the radioisotope-labeled technology, it was trying to predict the in vivo disposition properties, but different distribution results were revealed from a preliminary study in one human. ...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2018 Category: Drugs & Pharmacology Source Type: research

Candidate reference measurement procedure for determination of urea in serum by liquid chromatography-tandem mass spectrometry
This study investigated a candidate RMP (cRMP) for detecting serum urea directly via ID-liquid chromatography (LC)-MS/MS, without derivatization, which simplifies pre-processing samples. The cRMP was developed and evaluated relative to the recognized RMP, inter- and intra-laboratories. The intra-precisions were 1.35%, 1.98% and 1.47% at 4.95, 24.74 and 31.36 mmol/L, respectively; inter-precision was 2.10%, 2.60% and 2.10%. The relative bias for the measurement of standard human serum (SRM 909c) was –0.49%. The relative biases were 0.41% and 0.02% for IFCC-RELA (International Federation of Clinical Chemistry an...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2018 Category: Drugs & Pharmacology Source Type: research

New insights into the altered binding capacity of pharmaceutical-grade human serum albumin: Site-specific binding studies by induced CD spectroscopy
Publication date: Available online 12 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Anna Tramarin, Daniele Tedesco, Marina Naldi, Maurizio Baldassarre, Carlo Bertucci, Manuela BartoliniAbstractThe ADMET profile of drugs is strongly affected by human serum albumin (HSA), due to its leading role as carrier of poorly soluble compounds in plasma; a critical assessment of the binding capacity of HSA and the evaluation of binding competition between drugs are therefore pivotal for a reliable pharmacokinetic and pharmacodynamic characterization. In clinical practice, a potential source of impai...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2018 Category: Drugs & Pharmacology Source Type: research

Urinary and plasma metabolite differences detected by HPLC-ESI-QTOF-MS in systemic sclerosis patients
Publication date: Available online 11 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Álvaro Fernández-Ochoa, Rosa Quirantes-Piné, Isabel Borrás-Linares, David Gemperline, PRECISESADS Clinical Consortium, Marta E. Alarcón Riquelme, Lorenzo Beretta, Antonio Segura-CarreteroAbstractSystemic Sclerosis (SSc) is a chronic autoimmune disease whose origin and pathogenesis are not yet well known. Recent studies are allowing a better definition of the disease. However, few studies have been performed based on metabolomics. In this way, this study aims to find alte...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 12, 2018 Category: Drugs & Pharmacology Source Type: research

Quantification of isoflavone glycosides and aglycones in rat plasma by LC–MS/MS: Troubleshooting of interference from food and its application to pharmacokinetic study of Semen Sojae Praeparatum extract
Publication date: 30 November 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 161Author(s): Yuan Yao, Xin Ma, Teng Li, Hui Guo, Ruirui Chang, Jialin Liu, Qiaoxia Liu, Hongyuan Hao, Taohong Huang, Weidong Chen, Jun Wen, Tingting ZhouAbstractThe isoflavones widely exist in the daily diets and interferences are usually inevitable in the determination of the in vivo level of the same analytes. A new strategy to eliminate the dietary interference was established to evaluate the exposure of isoflavones including daidzin, glycitin, genistin, daidzein, glycitein, and genistein in rats fed with Semen Sojae Pra...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 12, 2018 Category: Drugs & Pharmacology Source Type: research

An ICP-MS platform for metal content assessment of cell culture media and evaluation of spikes in metal concentration on the quality of an IgG3:κ monoclonal antibody during production
Publication date: Available online 10 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Adil Mohammad, Cyrus Agarabi, Sarah Rogstad, Elizabeth DiCioccio, Kurt Brorson, Muhammad Ashraf, Patrick J. Faustino, Chikkathur N. MadhavaraoAbstractMetal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution. However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb. ...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2018 Category: Drugs & Pharmacology Source Type: research

A whole blood sample-to-answer polymer lab-on-a-chip with superhydrophilic surface toward point-of-care technology
Publication date: Available online 8 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kang Kug Lee, Myoung-Ok Kim, Seokheun ChoiABSTRACTAn innovative sample-to-answer (S-to-A) polymer lab-on-a-chip (LOC) with a blood plasma separator based on asymmetric capillary force has been proposed, developed, and completely characterized for point-of-care technology (POCT) applications. A spray layer-by-layer (LbL) nanoassembly coating has been applied for the superhydrophilic surface onto the cyclic olefin copolymer (COC). Then, the developed superwetting surfaces were designed and optimized for thre...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 9, 2018 Category: Drugs & Pharmacology Source Type: research

Diffusion ordered NMR spectroscopy measurements as screening method of potential reactions of API and excipients in drug formulations
Publication date: Available online 8 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alexander Becht, Curd Schollmayer, Johannes Wiest, Detlef heller, Wolfgang baumann, Helmut Buschmann, Ulrike HolzgrabeAbstractIn the development of new pharmaceutical formulations it is important to consider the possible interactions between the active pharmaceutical ingredient (API) and excipients which is a well-known problem. The object of the work presented here was to investigate such reactions by means of diffusion ordered NMR spectroscopy (DOSY). The known reaction of 5-aminosalicylic acid (5-ASA) a...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 9, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of simple step protein precipitation UHPLC-MS/MS methods for quantitation of temozolomide in patient plasma samples
Publication date: Available online 8 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed A. El Mubarak, Evgenios K. Stylos, Maria V. Chatziathanasiadou, Charikleia Danika, G.A. Alexiou, P. Tsekeris, Alexander Renziehausen, Tim Crook, Nelofer Syed, Gregory B. Sivolapenko, Andreas G. TzakosAbstractTemozolomide (TEMODAL™) (TMZ) is an antineoplastic agent that is primarily used for the treatment of glioblastoma and anaplastic gliomas, two aggressive forms of brain cancer. Due to the poor prognosis of brain tumour patients, there is an increasing body of research into improving the st...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 9, 2018 Category: Drugs & Pharmacology Source Type: research

Qualitative and quantitative analysis of Yifei Tongluo granules to identify main bioactive components using LC-DAD/MS and pharmacokinetic studies
This study provides a meaningful basis for evaluating the viability of Yifei Tongluo granules for clinical applications.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 8, 2018 Category: Drugs & Pharmacology Source Type: research

Development of Formaldehyde dehydrogenase-coupled assay and Antibody-based assays for ALKBH5 Activity Evaluation
Publication date: Available online 7 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dan-Dan Shen, Feng-Zhi Suo, Qi-Meng Song, Jiao Chang, Ting Zhang, Jing-Jing Hong, Yi-Chao Zheng, Hong-Min LiuAbstractN6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). Until now, two RNA demethylases have been identified, including FTO (fat mass and obesity-associated protein) and ALKBH5 (α-ketoglutarate-dependent dioxygenase alkB homologue 5). As a mammalian m6A demethylase, ALKBH5 significantly affects mRNA export and RNA metabolism as well as t...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 8, 2018 Category: Drugs & Pharmacology Source Type: research