Solid-state analysis of amorphous solid dispersions: why dsc and xrpd may not be regarded as stand-alone techniques
Publication date: Available online 18 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sien Dedroog, Timothy Pas, Bjorn Vergauwen, Christophe Huygens, Guy Van den MooterAbstractAmorphous solid dispersions (ASDs) are single-phase amorphous systems, where drug molecules are molecularly dispersed (dissolved) in a polymer matrix. The molecular dispersion of the drug molecules is responsible for their improved dissolution properties. Unambiguously establishing the phase behaviour of the ASDs is of utmost importance. In this paper, we focused on the complementary nature of (modulated) differential ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 20, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative Study on Metabolic Profiling and Excretion in Rat Bile Between Combination of Notoginseng Total Saponins and Safflower Total Flavonoids and Its Individual Extracts by LC-MS/MS
In conclusion, the compatibility of SF and NS could reduce the bile excretion of the CNS-derived compounds, which may be one of the reasons for the enhancement of anti-myocardial ischemia after combination.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 18, 2019 Category: Drugs & Pharmacology Source Type: research

Chromatographic tools for plant-derived recombinant antibodies purification and characterization
Publication date: Available online 14 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Caterina Temporini, Raffaella Colombo, Enrica Calleri, Sara Tengattini, Francesca Rinaldi, Gabriella MassoliniAbstractIn the last two decades, plants became an interesting alternative for the production of recombinant proteins for human therapy and several antibodies expressed in plants have reached the clinical development stage. Plants are capable of post-translational modifications (PTMs) necessary for protein activity and pharmacokinetics, such as glycosylation. However, there are important kingdom-spec...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of donepezil, 6-O-desmethyl donepezil and spinosin in beagle dog plasma using liquid chromatography‒tandem mass spectrometry and its application to a drug-drug interaction study
Publication date: Available online 13 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chae Bin Lee, Jee Sun Min, Soon Uk Chae, Hye Min Kim, Jun Hee Jang, In Ho Jung, Yu Fen Zheng, Jong Hoon Ryu, Soo Kyung BaeABSTRACTSpinosin, which is traditionally used for sedation and sleep disorders, has recently shown potential effects in alleviating memory loss. As spinosin is the main bioactive component in a standardized dried 50% ethanol extract of the seeds of Zizyphus jujuba var. spinosa, a Phase Ⅱb clinical trial is ongoing, in Korea for the combination of the above extract formulated in a table...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Separation Methods hyphenated to Mass Spectrometry for the Characterization of the Protein Glycosylation at the Intact Level
Publication date: Available online 13 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julien Camperi, Valerie Pichon, Nathalie DelaunayAbstractGlycosylation is one of the most common post-translational modifications of proteins that affects their biological activity, solubility, and half-life. Therefore, its characterization is of great interest in proteomic, particularly from a diagnostic and therapeutic point of view. However, the number and type of glycosylation sites, the degree of site occupancy and the different possible structures of glycans can lead to a very large number of isoforms...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolite distribution and correlation studies of Ziziphus jujuba and Ziziphus nummularia using LC-ESI-MS/MS
Publication date: Available online 11 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Muhammad Noman Khan, Faraz Ul Haq, Saeedur Rahman, Arslan Ali, Syed Ghulam MusharrafAbstractZiziphus jujuba and Ziziphus nummularia are two important species of the genus Ziziphus. Both plants offer great commercial value and are consumed as food around the world and used for their medicinal values such as anti-inflammatory, antioxidant and hepatoprotective activities. Comprehensive chemical profiling of Z. jujuba and Z. nummularia was done through identification of major metabolites using HPLC-QTOF-MS/MS a...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

Typing and determination of SNP functional gene based on highly selective and signal-amplified fluorescence double-probe with the help of ExoIII nuclease and magnetic bead
Publication date: Available online 11 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chun-Yan Min, Lu-Qian Wu, Ting-ting Qian, Noor Ul Ain, Wen-Juan Liu, Xiao-Ning Wu, Chen Zhang, Zhe Chen, Hong-Ping XieAbstractWe have developed a fluorescence double-probe detection system with signal amplification for simple typing and determination of single nucleotide polymorphism (SNP) functional gene based on non-sequence dependence of ExoIII nuclease on dsDNA and rapid separation of magnetic bead. Matched detected gene can cyclically release abundant fluorescence-labeled ssDNA from the probe and the c...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

Advanced sensing technologies of phenolic compounds for pharmaceutical and biomedical analysis
Publication date: Available online 11 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Liya Ge, Shao-Ping Li, Grzegorz LisakAbstractPhenolic compounds are bioactive natural products of considerable interest in pharmaceuticals and biomedicines. Due to their bioactive functions, phenolic compounds have received increasing attention in recent years. Therefore, it is necessary to develop new and advanced analytical methods for determination of phenolic compounds in relation to pharmaceutical and biomedical applications. However, in majority, their detection has been largely conducted by sensitive...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

NMR Reveals an Undeclared Constituent in Custom Synthetic Peptides
This report shows how NMR analysis uncovered the unanticipated presence of substantial amounts of mannitol (20 and 43% w/w) as undeclared constituent in two custom synthetic peptides, DR and DRVYI, that were sourced commercially. Quantitative 1H NMR (qHNMR) readily detected the contaminant, even on a 60 MHz benchtop instrument, and quantified the highly polar UV-transparent adulterant. Quantum-mechanical 1H iterative Full Spin Analysis (HiFSA) achieved unambiguous identification of both the mannitol and the peptides and confirmed the quantitative results.The cases show that experimental verification supersedes trust...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 11, 2019 Category: Drugs & Pharmacology Source Type: research

Detection of wheat allergens using 2D Western blot and mass spectrometry
ConclusionsThe present study allowed the identification of different protein areas specific to these studied groups. The protein spots of interest were identified by UHPLC-MS/MS. It has been possible to establish a link between a specific symptomatology and the newly identified responsible allergens. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 11, 2019 Category: Drugs & Pharmacology Source Type: research

Combinatory high-resolution microdissection/ultra performance liquid chromatographic–mass spectrometry approach for small tissue volume analysis of rat brain glycogen
Publication date: 30 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 178Author(s): Khaggeswar Bheemanapally, Mostafa M.H. Ibrahim, Karen P. BriskiAbstractCyto-architectural diversity of brain structures emphasizes need for analytical tools for discriminative investigation of distinctive neural structures. Glycogen is the major energy reserve in the brain. There is speculation that brain utilization of this fuel source may affect detection of hypoglycemia. To evaluate sex-specific regulation of glycogen mass and mobilization in the glucose-sensory ventromedial hypothalamic nucleus (VMN), curren...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 11, 2019 Category: Drugs & Pharmacology Source Type: research

ccImproving Diagnosis of Genitourinary Cancers: Biomarker Discovery Strategies through Mass Spectrometry-based Metabolomics
Publication date: Available online 7 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Malena Manzi, Gabriel Riquelme, Nicolás Zabalegui, María Eugenia MongeAb stractThe genitourinary oncology field needs integration of results from basic science, epidemiological studies, clinical and translational research to improve the current methods for diagnosis. MS-based metabolomics can be transformative for disease diagnosis and contribute to global health parity. Metabolite panels are promising to translate metabolomic findings into the clinics, changing the current diagnosis paradigm b...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 10, 2019 Category: Drugs & Pharmacology Source Type: research

On-line In-Tube Solid Phase Microextraction coupled to Capillary Liquid Chromatography-Diode Array Detection for the analysis of caffeine and its metabolites in small amounts of biological samples
Publication date: Available online 7 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): H.D. Ponce-Rodríguez, A.A. García-Robles, P. Sáenz-González, J. Verdú-Andrés, P. Campíns-FalcóAbstractIn-tube solid phase microextraction (IT-SPME) coupled on-line to capillary liquid chromatography with diode array detection provides a simple and fast analytical methodology for the simultaneous quantitation of caffeine and its three primary metabolites (theobromine, paraxanthine and theophylline) in micro samples of serum, saliva and urine matrices. Th...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 10, 2019 Category: Drugs & Pharmacology Source Type: research

Stability behaviour of antiretroviral drugs and their combinations. 11: Characterization of interaction products of zidovudine and efavirenz, and evaluation of their anti HIV-1 activity, and physiochemical and ADMET properties
Publication date: Available online 6 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Moolchand Kurmi, Archana Sahu, Ankit Balhara, Inder Pal Singh, Smita Kulkarni, Navneet Kumar Singh, Prabha Garg, Saranjit SinghAbstractZidovudine (ZDV) and efavirenz (EFV), which belong to two separate classes of antiretroviral drugs, viz., NRTI and NNRTI, respectively, were subjected to different stability test conditions alone and in solid mixtures to evaluate possibility of interaction among them. The exposed samples were analyzed by high performance liquid chromatography (HPLC) using a C18 column and a P...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 8, 2019 Category: Drugs & Pharmacology Source Type: research

Automated Open-Access Liquid Chromatography High Resolution Mass Spectrometry to Support Drug Discovery Projects
Publication date: Available online 5 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alberto Fontana, Laura Iturrino, David Corens, Antonio L. CregoAbstractThe need of a continuous productivity increases in medicinal chemistry laboratories of the pharmaceutical industry motivated the development, over the years, of new software solutions to enable Open-Access in many analytical techniques such as NMR or LC, among others, to characterize and assess the purity of new molecules. These approaches have been widely spread in LC with low resolution MS systems, but similar automated platforms have b...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 6, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of underivatized 17 β-estradiol using a high-throughput LC–MS/MS assay – Application to support a pharmacokinetic study in ovariectomized guinea pigs
Publication date: 30 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 178Author(s): Adarsh Gandhi, Murali K. Matta, Sharron Stewart, Ashok Chockalingam, Alan Knapton, Rodney Rouse, Wendy Wu, Vikram PatelAbstractDifference in female sex hormone, β-estradiol (E2), levels can contribute to sex differences in biological processes that underlie target tissue functions (QT interval), vulnerability to diseases (hepatitis or HIV), and response toward therapies. Accurate quantification of plasma E2 level is thus an important aspect in both basic science research examining hormone-regulated physiolo...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 6, 2019 Category: Drugs & Pharmacology Source Type: research

HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants
Publication date: Available online 4 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Cristina Romero Rocamora, Kalavathy Ramasamy, Siong Meng Lim, Abu Bakar Abdul Majeed, Snezana Agatonovic-KustrinAbstractA high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis (EDA) was developed to screen the antioxidant, neuroprotective and antidiabetic effects in essential oils derived from lavender flower, lemon myrtle, oregano, peppermint, sage, and rosemary leaves (Lamiaceae family). HPTLC hyphenated with microchemical (DPPH•, p-anisaldehyde, and ferric c...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Strategies for Stabilizing Formulation and QbD Assisted Development of Robust Stability Indicating Method of Azilsartan Medoxomil/Chlorthalidone
Publication date: Available online 4 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mohamed A. Gad, Sawsan M. Amer, Hala E. Zaazaa, Said A. HassanAbstractReasons for formulation instability were investigated either encountered during production or analytical processes of azilsartan medoxomil (AZM)/chlorthalidone hydrochloride (CLT) tablets. Through the identification of the most feasible degradation pathways, several strategies were proposed to enhance the stability of AZM/CLT formulation. Furthermore, a robust HPLC-UV method was developed and validated for the determination of AZM, CLT in ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Direct and Indirect Quantification of Phosphate Metabolites of Nucleoside Analogs in Biological Samples
Publication date: Available online 3 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Nagsen Gautam, Jawaher Abdullah Alamoudi, Sushil Kumar, Yazen AlnoutiAbstractNucleoside reverse transcriptase inhibitors (NRTIs) are prodrugs that require intracellular phosphorylation to active triphosphate nucleotide metabolites (NMs) for their pharmacological activity. However, monitoring these pharmacologically active NMs is challenging due to their instability, high hydrophilicity, and their low concentrations in blood and tissues. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is ...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Characterization of CYP2B6 K262R allelic variants by quantitative allele-specific proteomics using a QconCAT standard
Publication date: Available online 3 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jill Barber, Matthew R. Russell, Amin Rostami-Hodjegan, Brahim AchourAbstractClinically-relevant proteins are routinely characterized by targeted proteomic methods, which offer high accuracy and reproducibility. However, assays developed for these techniques lack distinction between different alleles expressed in biological samples. The significance of assessing such variations in genes relevant to pharmacology will depend on their prevalence and effects on drug therapy. We propose quantitative allele-specif...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative MALDI-MS/MS assay for serum cortisol through charged derivatization
In this study, we developed a method to quantify the serum CRT by MALDI-MS/MS. This method employed the derivatization using a Girard-type reagent, 1-(hydrazinocarbonylmethyl)isoquinolinium chloride, which compensated for the lack of sensitivity. This method enabled the reproducible quantification of the serum CRT using a 20-μL sample (intra- and inter-assay relative standard deviations, 7.4% or lower), and the measurable range was 25–500 ng/mL. The serum CRT concentrations determined by the newly-developed MALDI-MS/MS method agreed well with those by liquid chromatography/electrospray ionization-MS/MS or e...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of carbapenem antibiotics using a purpose-made capillary electrophoresis instrument with contactless conductivity detection
Publication date: Available online 3 October 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Thi Ngoc Mai Pham, Thai Binh Le, Duc Dung Le, Tran Hung Ha, Ngoc Son Nguyen, Tien Duc Pham, Peter C. Hauser, Thi Anh Huong Nguyen, Thanh Duc Mai (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 3, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolic profiling of seminal plasma from teratozoospermia patients
In this study, the analysis of samples performed by proton nuclear magnetic resonance spectroscopy (1H NMR spectroscopy) to identify the various metabolites, with the aim of finding metabolic profiles and biomarkers related to male infertility. Eighteen de-regulated metabolites were identified in fertile men compared to teratozoospermia patients. These changes illustrate the deficiencies in absorption or metabolism of these metabolites in teratozoospermia. Furthermore, metabolic profiling showed that it is not possible to classify teratozoospermia based on teratozoospermia index (TZI). To the best of our knowledge, this is...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 3, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of eight β-lactam antibiotics in human plasma and cerebrospinal fluid by liquid chromatography coupled to tandem mass spectrometry
The objective of this work is to develop and validate a fast and reliable method using liquid chromatography coupled to tandem mass spectrometric detection to quantify simultaneously amoxicillin, cloxacillin, cefazolin, cefotaxime, ceftazidime, cefepime, meropenem and piperacillin in plasma and cerebrospinal fluid (CSF).Sample clean-up included protein precipitation with acetonitrile followed by evaporation of the supernatant and reconstitution of the residue with mobile phase solvents. Eight deuterated β-lactam antibiotics were used as internal standards. Chromatographic separation was performed on a C18 column (50&t...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Theoretical mechanistic insight into the gabapentin lactamization by an intramolecular attack: Degradation model and stabilization factors
ConclusionsThe thermodynamic stability of the lactam form (-13.63 kcal/mol) clarifies the more thermal stability of gaba-L than its related gabapentin form and the experimental preference for the lactamization. The corresponding energy profile on protonation/deprotonation forms of gabapentin indicates the pH-dependent of the process and the rate reduction in out of the mild pH.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Combinatory High-Resolution Microdissection/Ultra Performance Liquid Chromatographic-Mass Spectrometry Approach for Small Tissue Volume Analysis of Rat Brain Glycogen
Publication date: Available online 30 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Khaggeswar Bheemanapally, Mostafa M.H. Ibrahim, Karen P. BriskiAbstractCyto-architectural diversity of brain structures emphasizes need for analytical tools for discriminative investigation of distinctive neural structures. Glycogen is the major energy reserve in the brain. There is speculation that brain utilization of this fuel source may affect detection of hypoglycemia. To evaluate sex-specific regulation of glycogen mass and mobilization in the glucose-sensory ventromedial hypothalamic nucleus (VMN),...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Advanced analytical methodologies in Alzheimer’s disease drug discovery
This article reviews the analytical methodologies developed and applied in the last five years to address the early-stage tasks of the AD drug discovery process: the fast selection of active compounds (hits) and the comprehension of the ligand binding mechanism of the compound chosen to be the lead in the forthcoming development. The reviewed analytical methodologies face the most investigated pharmacological protein targets (amyloids, secretases, kinases, cholinesterases) and specific receptor- and enzyme-mediated effects in neurotransmission, neuroprotection and neurodegeneration. Some of these methodologies are notewort...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 1, 2019 Category: Drugs & Pharmacology Source Type: research

Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus
Publication date: Available online 29 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yong-Gang Xia, Si-Miao Yu, Jun Liang, Bing-You Yang, Hai-Xue KuangABSTRACTA generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques. Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides. The corresponding digested products were efficiently separated and detected using GC-MS...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 1, 2019 Category: Drugs & Pharmacology Source Type: research

Physical and chemical stability of a generic etoposide formulation as an alternative to etoposide phosphate
In conclusion, ETP at concentrations of 0.68 and 1 mM prepared and stored at 25 °C under good manufacturing practices remained unchanged over a period of 21 days irrespective of the nature of the solvents or the type of container.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 1, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of underivatized 17 β-estradiol using a high-throughput LC-MS/MS assay – application to support a pharmacokinetic study in ovariectomized guinea pigs
Publication date: Available online 28 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Adarsh Gandhi, Murali K. Matta, Sharron Stewart, Ashok Chockalingam, Alan Knapton, Rodney Rouse, Wendy Wu, Vikram PatelAbstractDifference in female sex hormone, β-estradiol (E2), levels can contribute to sex differences in biological processes that underlie target tissue functions (QT interval), vulnerability to diseases (hepatitis or HIV), and response toward therapies. Accurate quantification of plasma E2 level is thus an important aspect in both basic science research examining hormone-regulated p...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 29, 2019 Category: Drugs & Pharmacology Source Type: research

N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: generation and application of a new glucose unit database
Publication date: Available online 28 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Csenge Filep, Beata Borza, Gabor Jarvas, Andras GuttmanABSTRACTAs glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins. Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance. This paper report...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 29, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a chemical fingerprint as a tool to distinguish closely related Tinospora species and quantitation of marker compounds
Publication date: Available online 27 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Abidah Parveen, Yan-Hong Wang, Omer Fantoukh, Manal Alhusban, Vijayasankar Raman, Zulfiqar Ali, Ikhlas A. KhanAbstractTinospora species are morphologically similar. Several cases of human toxicity have been reported in association with T. crispa. A chemical fingerprint was developed to differentiate T. crispa from its closely related species and to quantitate its major furanoditerpenes namely as borapetosides B, C and F. The rapid, sensitive and repeatable method was established using ultra-high-performan...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 30 November 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 176Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

How reliable is dietary supplement labelling?—Experiences from the analysis of ecdysterone supplements
Publication date: 5 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 177Author(s): Gabriella Ambrosio, Dave Wirth, Jan Felix Joseph, Monica Mazzarino, Xavier de la Torre, Francesco Botrè, Maria Kristina ParrAbstractThe present study aimed to design, develop, and optimize an analytical procedure to perform the quantitative determination of ecdysterone in commercially available dietary supplements. The newly developed procedure is based on the extraction of ecdysterone from the supplements and the subsequent analysis by an optimized UHPLC-MS/MS method. Chromatographic separation was perfor...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

Feasibility of rapidly assessing reactive impurities mediated excipient incompatibility using a new method: A case study of famotidine-PEG system
Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Isha Saraf, Dattatray Modhave, Varun Kushwah, Dmytro Neshchadin, Georg Gescheidt, Gert Trausinger, Philipp Melchior, Christoph Magnes, Amrit PaudelAbstractThe present work demonstrates the utility of temperature controlled set up with pressurized headspace oxygen as an approach to effectively reduce the time required for solid-state drug-excipient compatibility study. To illustrate the utility, the incompatibility of polyethylene glycol (PEG) and polyethylene oxide (PEO) with Famotidine (Fam) was shown. O...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

A Novel HPLC Method for Analysis of Atosiban and its Five Related Substances in Atosiban Acetate Injection
Publication date: Available online 25 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Qing Li, Yuye Huang, Yue Zhang, Huayuan Cui, Lining Yin, Yuqin Li, Ahu Yuan, Yiqiao Hu, Jinhui WuAbstractConsulting the national pharmacopoeia, no official quality standard was found for estimation of related substances and assay of atosiban acetate injection, of which main active component is atosiban. To solve this problem, herein, a novel high performance liquid chromatographic (HPLC) method was developed and validated in this study. A chromatographic system comprising an Inertsil ODS-2 analytical colu...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 26, 2019 Category: Drugs & Pharmacology Source Type: research

Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine
Publication date: Available online 25 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xinyu Huang, Tng Huang, Xujiang Li, Zibing HuangAbstractA flower-like gold nanoparticles (FGN)-based immunochromatographic test strip (ICS) was developed and used for the first time for the rapid simultaneous detection of fumonisin B1 (FB1) and deoxynivalenol (DON) in Chinese traditional medicine. Several experimental conditions affecting the sensitivity of ICS have been investigated, including the type of FGN, the preparation conditions of FGN-monoclonal antibody (MAb) conjugates, and the process paramet...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 26, 2019 Category: Drugs & Pharmacology Source Type: research

Application of separation methods for in vitro prediction of blood–brain barrier permeability—The state of the art
Publication date: Available online 22 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Krzesimir Ciura, Szymon DziombaAbstractDespite many efforts, drug discovery pipeline is still a highly inefficient process. Nowadays, when combinatorial chemistry enables to synthesize hundreds of new drugs candidates, methods for rapid assessment of biopharmaceutical parameters of new compounds are highly desired. Over one-third of drugs candidates is rejected because of unsatisfactory pharmacokinetic properties. In the drug discovery process, the blood-brain barrier (BBB) permeability plays a critical r...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 23, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of cystatin c in human urine by isotope dilution tandem mass spectrometry
Publication date: Available online 22 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Amanda Suárez-Fernández, Ana González-Antuña, Pablo Rodríguez-González, J. Ignacio García Alonso.AbstractThis work presents the development of a methodology for the accurate and precise quantification of the renal biomarker Cystatin C in human urine by Isotope Dilution Mass Spectrometry (IDMS). The procedure is based on the addition of a known quantity of the proteotypic peptide ALDFAVG*EYNK labelled with 13C2-glycine to the urine sample followed by protein...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 23, 2019 Category: Drugs & Pharmacology Source Type: research

Validation and evaluation of a newly developed time-resolved fluoroimmunoassay for cyclosporine quantitative analysis in human whole blood
Publication date: Available online 22 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xuebin Wang, Yuan gao, Yunyun Yang, Zhengyue Liu, Lihong Gao, Fengbo Wu, Xianmi Yang, Xiaojian Xu, Zhuo Wang, Shusen SunAbstractVarious immunoassay methods have been developed and used for the therapeutic drug monitoring (TDM) of cyclosporine (CsA). However, there is no report on the application of a time-resolved fluoroimmunoassay (TRFIA) in routine CsA TDM. The aim of this study was to evaluate the feasibility and validate the performance of a newly developed TRFIA method for CsA analysis in human whole...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 23, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic comparative study of tetramethylpyrazine and ferulic acid and their compatibility with different concentration of gastrodin and gastrodigenin on blood–stasis migraine model by blood–brain microdialysis method
Publication date: Available online 21 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yahui Mi, Siyu Guo, Huan Cheng, Mingping Liu, Pinqing Wei, Meijing Wang, Yukang Mao, Guohan KeAbstractTianma pills, a traditional formula made from Ligusticum chuanxiong and Gastrodia elata, are efficacious for the treatment of primary headache. Tetramethylpyrazine (TMP) and Ferulic acid (FA) are the bioactive ingredients of Ligusticum chuanxiong, while Gastrodin and Gastrodigenin are the bioactive ingredients of Gastrodia elata. Pharmacokinetic assessment of TMP, FA, gastrodin or gastrodigenin in blood o...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2019 Category: Drugs & Pharmacology Source Type: research

Automated fluorimetric determination of the genotoxic impurity hydrazine in allopurinol pharmaceuticals using zone fluidics and on-line solid phase extraction
Publication date: Available online 21 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Paraskevas D. Tzanavaras, Stefanos Themistokleous, Constantinos K. ZacharisAbstractIn the present study we report a fully automated method for the determination of low levels of the genotoxic impurity hydrazine in allopurinol active pharmaceutical ingredient (API) and formulations based on the concept of zone-fluidics. Hydrazine reacts on-line with o-phthalaldehyde in a unique way, that is in acidic medium (pH 
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2019 Category: Drugs & Pharmacology Source Type: research

Comprehensive Metabolic Profiles of Seminal Plasma with Different Forms of Male Infertility and Their Correlation with Sperm Parameters
Publication date: Available online 21 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yamei Xu, Hongmei Lu, Yang Wang, Zhimin Zhang, Wu QianAbstractMetabolomics measurements of seminal plasma are widely used in diagnosis and finding of molecular mechanisms of male infertility. However, so far the limitation of metabolome coverage of analytical methods hinders comprehensive metabolite biomarker finding. Moreover, the widely used case-control comparison is not enough to unveil the detailed correlations of the metabolic changes with different sperm abnormalities. In this work, we aimed to hav...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2019 Category: Drugs & Pharmacology Source Type: research

Quantification of a Cocrystal and its Dissociated Compounds in Solid Dosage Form Using Transmission Raman Spectroscopy
Publication date: Available online 18 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tatsuo Koide, Yuki Takeuchi, Takashi Otaki, Katsuhiko Yamamoto, Rie Shimamura, Ryo Ohashi, Motoki Inoue, Toshiro Fukami, Ken-ichi IzutsuAbstractThe performance of transmission Raman spectroscopy (TRS) for quantifying a cocrystal and its dissociation in solid dosage form was investigated. Some tablets containing 0%–20% (w/w) of a cocrystal of carbamazepine (CBZ)/succinic acid (SUC), 0%–4% of CBZ, 0%–4% of SUC, and 75%–99% of D-mannitol were prepared. The Raman spectra of these table...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 19, 2019 Category: Drugs & Pharmacology Source Type: research

Microextraction of aromatic microbial metabolites by packed hypercrosslinked polystyrene from blood serum
Publication date: Available online 14 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alisa K. Pautova, Pavel D. Sobolev, Alexander I. RevelskyAbstractThe article is devoted to the application of modern sample preparation technique - microextraction by packed sorbent (MEPS) - in conjunction with non-conventional type of sorbent - hypercrosslinked polystyrene, that was investigated for the first time in this work. Their combination was used to extract phenylcarboxylic acid-type aromatic microbial metabolites from serum samples of a healthy volunteer with following derivatization and GC-MS d...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 15, 2019 Category: Drugs & Pharmacology Source Type: research

Demonstration of real-time and accelerated stability of hepatitis E vaccine with a combination of different physicochemical and immunochemical methods
Publication date: Available online 13 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaochen Yin, Xin Wang, Zhigang Zhang, Yufang Li, Zhijie Lin, Huirong Pan, Ying Gu, Shaowei Li, Jun Zhang, Ningshao Xia, Qinjian Zhao (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of raltegravir and raltegravir glucuronide in human plasma and urine by LC–MS/MS with application in a maternal-fetal pharmacokinetic study
Publication date: 5 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 177Author(s): Fernanda de Lima Moreira, Maria Paula Marques, Geraldo Duarte, Vera Lucia LanchoteAbstractRaltegravir (RAL) is a HIV-integrase inhibitor recommended for treatment of HIV type 1 infection during pregnancy. The elimination of RAL to RAL glucuronide (RAL GLU) is mediated primarily by UDP glucuronosyltransferase 1A1 (UGT1A1). The present study shows the development and validation of 4 different methods for the analysis of RAL and RAL GLU in plasma and in urine samples. The methods were applied to evaluate the materna...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

Clinical and Pharmaceutical Applications of Affinity Ligands in Capillary Electrophoresis: A Review
Publication date: Available online 12 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, David S. HageAbstractAffinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents th...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative pharmacokinetic study of the components in Alpinia oxyphylla Miq.-Schisandra chinensis (Turcz.) Baill. herb pair and its single herb between normal and Alzheimer’s disease rats by UPLC-MS/MS
This study showed ASHP can improve the absorption of protocatechuic acid, chrysin, schisandrin, gomisin B, nootkatone, deoxyschizandrin, schisandrin B and schisandrin C in vivo and slow down part of these components’ elimination. In addition, compared with normal rats, the pharmacokinetic parameters changed significantly in AD model rats’ plasma after oral administration of ASHP. Hence, these may be the pharmacokinetic mechanism of ASHP, in addition to serving as a potential agent in the treatment of AD. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

How reliable is dietary supplement labelling? – Experiences from the analysis of Ecdysterone supplements
Publication date: Available online 11 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gabriella Ambrosio, David Wirth, Jan Felix Joseph, Monica Mazzarino, Xavier de la Torre, Francesco Botrè, Maria Kristina ParrAbstractThe present study aimed to design, develop, and optimize an analytical procedure to perform the quantitative determination of ecdysterone in commercially available dietary supplements. The newly developed procedure is based on the extraction of ecdysterone from the supplements and the subsequent analysis by an optimized UHPLC-MS/MS method. Chromatographic separation w...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 12, 2019 Category: Drugs & Pharmacology Source Type: research