Chemical fingerprinting techniques for the differentiation of polysaccharides from genus Astragalus
Publication date: Available online 29 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yong-Gang Xia, Si-Miao Yu, Jun Liang, Bing-You Yang, Hai-Xue KuangABSTRACTA generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques. Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides. The corresponding digested products were efficiently separated and detected using GC-MS...
Source: Journal of Pharmaceutical and Biomedical Analysis - October 1, 2019 Category: Drugs & Pharmacology Source Type: research

Physical and chemical stability of a generic etoposide formulation as an alternative to etoposide phosphate
In conclusion, ETP at concentrations of 0.68 and 1 mM prepared and stored at 25 °C under good manufacturing practices remained unchanged over a period of 21 days irrespective of the nature of the solvents or the type of container.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - October 1, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of underivatized 17 β-estradiol using a high-throughput LC-MS/MS assay – application to support a pharmacokinetic study in ovariectomized guinea pigs
Publication date: Available online 28 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Adarsh Gandhi, Murali K. Matta, Sharron Stewart, Ashok Chockalingam, Alan Knapton, Rodney Rouse, Wendy Wu, Vikram PatelAbstractDifference in female sex hormone, β-estradiol (E2), levels can contribute to sex differences in biological processes that underlie target tissue functions (QT interval), vulnerability to diseases (hepatitis or HIV), and response toward therapies. Accurate quantification of plasma E2 level is thus an important aspect in both basic science research examining hormone-regulated p...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 29, 2019 Category: Drugs & Pharmacology Source Type: research

N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: generation and application of a new glucose unit database
Publication date: Available online 28 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Csenge Filep, Beata Borza, Gabor Jarvas, Andras GuttmanABSTRACTAs glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins. Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance. This paper report...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 29, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a chemical fingerprint as a tool to distinguish closely related Tinospora species and quantitation of marker compounds
Publication date: Available online 27 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Abidah Parveen, Yan-Hong Wang, Omer Fantoukh, Manal Alhusban, Vijayasankar Raman, Zulfiqar Ali, Ikhlas A. KhanAbstractTinospora species are morphologically similar. Several cases of human toxicity have been reported in association with T. crispa. A chemical fingerprint was developed to differentiate T. crispa from its closely related species and to quantitate its major furanoditerpenes namely as borapetosides B, C and F. The rapid, sensitive and repeatable method was established using ultra-high-performan...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 30 November 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 176Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

How reliable is dietary supplement labelling?—Experiences from the analysis of ecdysterone supplements
Publication date: 5 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 177Author(s): Gabriella Ambrosio, Dave Wirth, Jan Felix Joseph, Monica Mazzarino, Xavier de la Torre, Francesco Botrè, Maria Kristina ParrAbstractThe present study aimed to design, develop, and optimize an analytical procedure to perform the quantitative determination of ecdysterone in commercially available dietary supplements. The newly developed procedure is based on the extraction of ecdysterone from the supplements and the subsequent analysis by an optimized UHPLC-MS/MS method. Chromatographic separation was perfor...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

Feasibility of rapidly assessing reactive impurities mediated excipient incompatibility using a new method: A case study of famotidine-PEG system
Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Isha Saraf, Dattatray Modhave, Varun Kushwah, Dmytro Neshchadin, Georg Gescheidt, Gert Trausinger, Philipp Melchior, Christoph Magnes, Amrit PaudelAbstractThe present work demonstrates the utility of temperature controlled set up with pressurized headspace oxygen as an approach to effectively reduce the time required for solid-state drug-excipient compatibility study. To illustrate the utility, the incompatibility of polyethylene glycol (PEG) and polyethylene oxide (PEO) with Famotidine (Fam) was shown. O...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 28, 2019 Category: Drugs & Pharmacology Source Type: research

A Novel HPLC Method for Analysis of Atosiban and its Five Related Substances in Atosiban Acetate Injection
Publication date: Available online 25 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Qing Li, Yuye Huang, Yue Zhang, Huayuan Cui, Lining Yin, Yuqin Li, Ahu Yuan, Yiqiao Hu, Jinhui WuAbstractConsulting the national pharmacopoeia, no official quality standard was found for estimation of related substances and assay of atosiban acetate injection, of which main active component is atosiban. To solve this problem, herein, a novel high performance liquid chromatographic (HPLC) method was developed and validated in this study. A chromatographic system comprising an Inertsil ODS-2 analytical colu...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 26, 2019 Category: Drugs & Pharmacology Source Type: research

Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine
Publication date: Available online 25 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xinyu Huang, Tng Huang, Xujiang Li, Zibing HuangAbstractA flower-like gold nanoparticles (FGN)-based immunochromatographic test strip (ICS) was developed and used for the first time for the rapid simultaneous detection of fumonisin B1 (FB1) and deoxynivalenol (DON) in Chinese traditional medicine. Several experimental conditions affecting the sensitivity of ICS have been investigated, including the type of FGN, the preparation conditions of FGN-monoclonal antibody (MAb) conjugates, and the process paramet...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 26, 2019 Category: Drugs & Pharmacology Source Type: research

Application of separation methods for in vitro prediction of blood–brain barrier permeability—The state of the art
Publication date: Available online 22 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Krzesimir Ciura, Szymon DziombaAbstractDespite many efforts, drug discovery pipeline is still a highly inefficient process. Nowadays, when combinatorial chemistry enables to synthesize hundreds of new drugs candidates, methods for rapid assessment of biopharmaceutical parameters of new compounds are highly desired. Over one-third of drugs candidates is rejected because of unsatisfactory pharmacokinetic properties. In the drug discovery process, the blood-brain barrier (BBB) permeability plays a critical r...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 23, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of cystatin c in human urine by isotope dilution tandem mass spectrometry
Publication date: Available online 22 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Amanda Suárez-Fernández, Ana González-Antuña, Pablo Rodríguez-González, J. Ignacio García Alonso.AbstractThis work presents the development of a methodology for the accurate and precise quantification of the renal biomarker Cystatin C in human urine by Isotope Dilution Mass Spectrometry (IDMS). The procedure is based on the addition of a known quantity of the proteotypic peptide ALDFAVG*EYNK labelled with 13C2-glycine to the urine sample followed by protein...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 23, 2019 Category: Drugs & Pharmacology Source Type: research

Validation and evaluation of a newly developed time-resolved fluoroimmunoassay for cyclosporine quantitative analysis in human whole blood
Publication date: Available online 22 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xuebin Wang, Yuan gao, Yunyun Yang, Zhengyue Liu, Lihong Gao, Fengbo Wu, Xianmi Yang, Xiaojian Xu, Zhuo Wang, Shusen SunAbstractVarious immunoassay methods have been developed and used for the therapeutic drug monitoring (TDM) of cyclosporine (CsA). However, there is no report on the application of a time-resolved fluoroimmunoassay (TRFIA) in routine CsA TDM. The aim of this study was to evaluate the feasibility and validate the performance of a newly developed TRFIA method for CsA analysis in human whole...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 23, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetic comparative study of tetramethylpyrazine and ferulic acid and their compatibility with different concentration of gastrodin and gastrodigenin on blood–stasis migraine model by blood–brain microdialysis method
Publication date: Available online 21 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yahui Mi, Siyu Guo, Huan Cheng, Mingping Liu, Pinqing Wei, Meijing Wang, Yukang Mao, Guohan KeAbstractTianma pills, a traditional formula made from Ligusticum chuanxiong and Gastrodia elata, are efficacious for the treatment of primary headache. Tetramethylpyrazine (TMP) and Ferulic acid (FA) are the bioactive ingredients of Ligusticum chuanxiong, while Gastrodin and Gastrodigenin are the bioactive ingredients of Gastrodia elata. Pharmacokinetic assessment of TMP, FA, gastrodin or gastrodigenin in blood o...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2019 Category: Drugs & Pharmacology Source Type: research

Automated fluorimetric determination of the genotoxic impurity hydrazine in allopurinol pharmaceuticals using zone fluidics and on-line solid phase extraction
Publication date: Available online 21 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Paraskevas D. Tzanavaras, Stefanos Themistokleous, Constantinos K. ZacharisAbstractIn the present study we report a fully automated method for the determination of low levels of the genotoxic impurity hydrazine in allopurinol active pharmaceutical ingredient (API) and formulations based on the concept of zone-fluidics. Hydrazine reacts on-line with o-phthalaldehyde in a unique way, that is in acidic medium (pH 
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2019 Category: Drugs & Pharmacology Source Type: research

Comprehensive Metabolic Profiles of Seminal Plasma with Different Forms of Male Infertility and Their Correlation with Sperm Parameters
Publication date: Available online 21 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yamei Xu, Hongmei Lu, Yang Wang, Zhimin Zhang, Wu QianAbstractMetabolomics measurements of seminal plasma are widely used in diagnosis and finding of molecular mechanisms of male infertility. However, so far the limitation of metabolome coverage of analytical methods hinders comprehensive metabolite biomarker finding. Moreover, the widely used case-control comparison is not enough to unveil the detailed correlations of the metabolic changes with different sperm abnormalities. In this work, we aimed to hav...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 22, 2019 Category: Drugs & Pharmacology Source Type: research

Quantification of a Cocrystal and its Dissociated Compounds in Solid Dosage Form Using Transmission Raman Spectroscopy
Publication date: Available online 18 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tatsuo Koide, Yuki Takeuchi, Takashi Otaki, Katsuhiko Yamamoto, Rie Shimamura, Ryo Ohashi, Motoki Inoue, Toshiro Fukami, Ken-ichi IzutsuAbstractThe performance of transmission Raman spectroscopy (TRS) for quantifying a cocrystal and its dissociation in solid dosage form was investigated. Some tablets containing 0%–20% (w/w) of a cocrystal of carbamazepine (CBZ)/succinic acid (SUC), 0%–4% of CBZ, 0%–4% of SUC, and 75%–99% of D-mannitol were prepared. The Raman spectra of these table...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 19, 2019 Category: Drugs & Pharmacology Source Type: research

Microextraction of aromatic microbial metabolites by packed hypercrosslinked polystyrene from blood serum
Publication date: Available online 14 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alisa K. Pautova, Pavel D. Sobolev, Alexander I. RevelskyAbstractThe article is devoted to the application of modern sample preparation technique - microextraction by packed sorbent (MEPS) - in conjunction with non-conventional type of sorbent - hypercrosslinked polystyrene, that was investigated for the first time in this work. Their combination was used to extract phenylcarboxylic acid-type aromatic microbial metabolites from serum samples of a healthy volunteer with following derivatization and GC-MS d...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 15, 2019 Category: Drugs & Pharmacology Source Type: research

Demonstration of real-time and accelerated stability of hepatitis E vaccine with a combination of different physicochemical and immunochemical methods
Publication date: Available online 13 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaochen Yin, Xin Wang, Zhigang Zhang, Yufang Li, Zhijie Lin, Huirong Pan, Ying Gu, Shaowei Li, Jun Zhang, Ningshao Xia, Qinjian Zhao (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of raltegravir and raltegravir glucuronide in human plasma and urine by LC–MS/MS with application in a maternal-fetal pharmacokinetic study
Publication date: 5 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 177Author(s): Fernanda de Lima Moreira, Maria Paula Marques, Geraldo Duarte, Vera Lucia LanchoteAbstractRaltegravir (RAL) is a HIV-integrase inhibitor recommended for treatment of HIV type 1 infection during pregnancy. The elimination of RAL to RAL glucuronide (RAL GLU) is mediated primarily by UDP glucuronosyltransferase 1A1 (UGT1A1). The present study shows the development and validation of 4 different methods for the analysis of RAL and RAL GLU in plasma and in urine samples. The methods were applied to evaluate the materna...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

Clinical and Pharmaceutical Applications of Affinity Ligands in Capillary Electrophoresis: A Review
Publication date: Available online 12 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, David S. HageAbstractAffinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents th...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative pharmacokinetic study of the components in Alpinia oxyphylla Miq.-Schisandra chinensis (Turcz.) Baill. herb pair and its single herb between normal and Alzheimer’s disease rats by UPLC-MS/MS
This study showed ASHP can improve the absorption of protocatechuic acid, chrysin, schisandrin, gomisin B, nootkatone, deoxyschizandrin, schisandrin B and schisandrin C in vivo and slow down part of these components’ elimination. In addition, compared with normal rats, the pharmacokinetic parameters changed significantly in AD model rats’ plasma after oral administration of ASHP. Hence, these may be the pharmacokinetic mechanism of ASHP, in addition to serving as a potential agent in the treatment of AD. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 14, 2019 Category: Drugs & Pharmacology Source Type: research

How reliable is dietary supplement labelling? – Experiences from the analysis of Ecdysterone supplements
Publication date: Available online 11 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gabriella Ambrosio, David Wirth, Jan Felix Joseph, Monica Mazzarino, Xavier de la Torre, Francesco Botrè, Maria Kristina ParrAbstractThe present study aimed to design, develop, and optimize an analytical procedure to perform the quantitative determination of ecdysterone in commercially available dietary supplements. The newly developed procedure is based on the extraction of ecdysterone from the supplements and the subsequent analysis by an optimized UHPLC-MS/MS method. Chromatographic separation w...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 12, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of four flavonoids in rat plasma after oral administration of Malus hupehensis (Pamp.) Rehd. extracts by UPLC‐MS/MS and its application to a pharmacokinetics study
Publication date: Available online 10 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dongyan Guo, Juanli Dang, Hui Yang, Yu Fan, Jiangxue Cheng, Yajun Shi, Xiaofei Zhang, Junbo ZouAbstractA rapid, sensitive, selective, and accurate UPLC–MS/MS method was developed and fully validated for the simultaneous determination of quercitrin, phloridzin, quercetin, and phloretin in rat plasma after oral administration of Malus hupehensis (Pamp.) Rehd extracts. The pharmacokinetic parameters of oral phloridzin monomer and phloridzin in the extract were also compared. Plasma samples were process...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative analysis of the main active constituents from different parts of Leonurus japonicus Houtt. and from different regions in China by ultra-high performance liquid chromatography with triple quadrupole tandem mass spectrometry
Publication date: Available online 10 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ya-Jie Tan, Ding-Qiao Xu, Shi-Jun Yue, Yu-Ping Tang, Sheng Guo, Hui Yan, Jing Zhang, Zhen-Hua Zhu, Xu-Qin Shi, Yan-Yan Chen, Yao Gu, Xiao-Rui Ding, Sheng-Liang Huang, Guo-Ping Peng, Gui-Sheng Zhou, Jin-Ao DuanAbstractA rapid, sensitive and convenient analytical method of ultra-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UPLC-QTRAP®/MS2) was proposed for the simultaneous determination of characteristic alkaline and acidic components coverin...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

LC-ESI-QTOF-MS analysis utilizing gas-phase fragmentation reactions subjected to ESI-IS-CID and ESI-CID-MS/MS conditions to study the degradation behaviour of sorafenib tosylate: NMR and in vitro cytotoxicity and apoptosis detection studies of hydrolytic degradation products
Publication date: Available online 10 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): P. Johnsirani, Aabid Abdullah Wani, Prasad V Bharatam, Satheeshkumar NanjappanAbstractThe present study was to investigate the degradation profile of sorafenib tosylate (SORA), a potent oral multi-kinase inhibitor under various stress conditions as per ICH (Q1A (R2)) guidelines. Separation of SORA and its degradation products (DP-1 to DP-5) was achieved on Acquity UPLC BEH C18 (100 mm x 2.1 mm x1.7 µm) column using a gradient elution of 0.1% formic acid and acetoni...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of azathioprine/6-mercaptopurine metabolites IN dried blood spots: correlation with RBCconcentrations
Publication date: Available online 10 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mervat AlsousAbstractThe analysis of 6-thioguanine (6-TG) and 6-methylmercaptopurine (6-mMP) in biological samples is not straight forward and requires pre-treatment of samples. There are no validated published methods for the analysis of azathioprine/6-mercaptopurine (AZA/6-MP) metabolites in dried blood spot (DBS) samples that study the correlation with red blood cells (RBC) concentrations. DBS was prepared by applying fifteen microliters of blood [spiked with analytes or samples obtained from patients]...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Optimization of the extraction procedure for the determination of phenolic acids and flavonoids in the leaves of globe artichoke (Cynara cardunculus var. scolymusL.)
In this study, an ultrasound-assisted extraction method was studied, which proved to be more efficient than the standard protocol of the European Pharmacopoeia, since it led to comparable results, was faster and easier to handle, and was more sustainable due to a reduced need for organic solvents. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics, bioavailability, excretion, and metabolic analysis of Schisanlactone E, a bioactive ingredient from Kadsura heteroclita (Roxb) Craib, in rats by UHPLC-MS/MS and UHPLC-Q-Orbitrap HRMS
This study provides comprehensive insights into the pharmacokinetic and metabolic profiles of SE, and would be valuable for future development and utilization of SE and Kadsura heteroclita.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Exploring the effective materials of flavonoids-enriched extract from Scutellaria baicalensis roots based on the metabolic activation in influenza A virus induced acute lung injury
Publication date: Available online 9 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): HaiJuan Zhi, Xin Jin, HaiYan Zhu, Hong Li, YunYi Zhang, Yan Lu, DaoFeng ChenABSTRACTFlavonoids-enriched extract from Scutellaria baicalensis roots (FESR) ameliorated influenza A virus (IAV) induced acute lung injury (ALI) in mice by inhibiting the excessive activation of complement system in vivo. However, FESR had no anti-complementary activity in vitro. In order to reveal the effective materials of FESR for the treatment of IAV-induced ALI, the present research explored the metabolic process of FESR both...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

A novel UHPLC-HRMS-based metabolomics strategy enables the discovery of potential neuroactive metabolites in mice plasma, following i.p. administration of the main Crocus sativus L. bioactive component
We report a novel untargeted UHPLC-ESI HRMS metabolomics strategy to determine the alteration of the metabolic fingerprint following i.p. administration of TC4 in male and female mice. Blood samples from fiftysix mice treated with TC4 as well as from control animals were analyzed with UHPLC-ESI HRMS. Statistical evaluation of the results was achieved by multivariate analysis (MVA), i.e., principal component analysis (PCA), Partial Least Squares − Discriminant Analysis (PLS-DA) in order to discover the variables that contributed to the discrimination between treated and untreated groups which were identi...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Falsified vardenafil tablets available online
In this study, we purchased vardenafil tablets on the internet and investigated their quality and authenticity using visual observations, authenticity investigations, non-destructive tests (handheld NIR and Raman spectroscopy), and quality analyses (active ingredient content and tablet dissolution rate). We used genuine 20-mg Levitra tablets that were sold in Japan and tablets from Bayer AG (Germany) as controls. In April 2015, we obtained 28 samples from 15 websites on the internet. Our authenticity investigations revealed that 11 (40%) were genuine products and 17 (60%) were falsified products. Handheld NIR and Raman res...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Bioanalysis of EGFRm inhibitor osimertinib, and its glutathione cycle- and desmethyl metabolites by liquid chromatography-tandem mass spectrometry
Publication date: Available online 10 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): J.J.M. Rood, M.J. van Haren, J.H. Beijnen, R.W. SparidansAbstractOsimertinib is a ‘‘third-generation’’ oral, irreversible, tyrosine kinase inhibitor. It is used in the treatment of non-small cellular lung carcinoma and spares wild-type EGFR. Due to its reactive nature, osimertinib is, in addition to oxidative routes, metabolized through GSH coupling and subsequent further metabolism of these conjugates. The extent of the non-oxidative metabolism of osimertinib is unknown, and metho...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 11, 2019 Category: Drugs & Pharmacology Source Type: research

Quality consistency evaluation of Kudiezi Injection based on multivariate statistical analysis of the multidimensional chromatographic fingerprint
In conclusion, multidimensional chromatographic fingerprints could reflect the quality information of KDZI comprehensively and hierarchical clustering analysis was suitable to identify the differences among batches. This could provide an integrated method for consistency evaluation of TCMI, process improvement of TCMI and solving similar problems in TCMI. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - September 9, 2019 Category: Drugs & Pharmacology Source Type: research

Assessment of gut microbiota fecal metabolites by chromatographic targeted approaches
This article reviews the literature on targeted chromatographic approaches in analysis of gut microbiota specific metabolites in feces as the most accessible biological matrix which can directly probe the connection between intestinal bacteria and the (patho)physiology of the holobiont. Together with a discussion on sample collection and preparation, the chromatographic methods targeted to determination of some classes of microbiota-derived metabolites (e.g., short-chain fatty acids, bile acids, low molecular masses amines and polyamines, vitamins, neurotransmitters and related compounds) are discussed and their main chara...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 9, 2019 Category: Drugs & Pharmacology Source Type: research

Pilot study of homeostatic alterations of mineral elements in serum of patients with age-related macular degeneration via elemental and isotopic analysis using ICP-mass spectrometry
Publication date: Available online 7 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marta Aranaz, Marta Costas-Rodríguez, Lara Lobo, Héctor González-Iglesias, Frank Vanhaecke, Rosario PereiroAbstractAge-related macular degeneration (AMD), the main cause of irreversible blindness in people over 60 years of age, is an eye disease that evolves with loss of central vision. Although AMD manifests itself in the eye, blood is continuously flowing through the macular region, such that potential alterations in this region could be reflected in the composition of whole blood or...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 8, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of endogenous epinephrine and norepinephrine enantiomers in rat plasma and application to a stereoselective pharmacokinetics
Publication date: Available online 6 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Huijie Lv, Siqi Luo, Ruochen Tian, Wenhua Zhang, Baoshan Sun, Yan CuiAbstractEpinephrine and norepinephrine are a class of chiral endogenous catecholamines, which are known as major neurotransmitters. This work described a new LC-MS/MS method coupled with pre-column derivatization, enabling the simultaneous enantiomeric separation of epinephrine and norepinephrine in rat plasma. After protein precipitation procedure, the samples were derivatized with (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethy...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 6, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a robust reporter gene-based assay for the bioactivity determination of recombinant human follicle stimulating hormone (rhFSH) pharmaceutical products
Publication date: Available online 4 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lv-yin Wang, Cheng-gang Liang, Hui-min Yang, Jing Li, Zhan-jun Li, Hui Zhang, Ping Lv, Yi Li, Jun-zhi WangAbstractFSH plays a key role in the function of the reproductive system of human beings and is widely used both diagnostically and therapeutically in reproductive medicine. With the growing incidence of infertility, the demand for FSH pharmaceutical products is increasing. For this reason, the quality control process for FSH products is becoming more stringent. An accurate determination of bioactivity ...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 5, 2019 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography-tandem mass spectrometric method for the quantification of eliglustat in rat plasma and the application in a pre-clinical study
Publication date: Available online 4 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jingjing Chen, Yuanyuan Shao, Huidan Zhu, Xiufang Chen, Xuemei YeAbstractEliglustat is an oral substrate reduction therapy drug and has been approved as a first-line treatment for adults with Gaucher disease type 1 (GD 1). In the present study, we aimed to develop and establish an accurate and simple ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the measurement of eliglustat concentration in rat plasma. The goal of chromatographic separation of eliglustat and the ...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 5, 2019 Category: Drugs & Pharmacology Source Type: research

Identification of the metabolites in normal and AA rat plasma, urine and feces after oral administration of Daphne genkwa flavonoids by LC-Q-TOF-MS spectrometry
In this study, we analyzed total flavonoids in D. genkwa and their metabolites in normal and adjuvant arthritis (AA) rat plasma, urine and feces samples by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). A total of 4 metabolites in plasma, 9 metabolites in urine and 15 metabolites in feces were characterized respectively by LC-Q-TOF-MS technology in normal rat. And 9 of the metabolites were observed in the AA rat urine, while there was no prototype drug or its metabolites detected in plasma and fecal samples. The metabolic pathway mainly involves hydroxylation, methylation, glu...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 5, 2019 Category: Drugs & Pharmacology Source Type: research

Evaluation of Statistical Techniques to Normalize Mass Spectrometry-Based Urinary Metabolomics Data
Publication date: Available online 3 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tyler Cook, Yinfa Ma, Sanjeewa GamagedaraAbstractHuman urine recently became a popular medium for metabolomics biomarker discovery because its collection is non-invasive. Sometimes renal dilution of urine can be problematic in this type of urinary biomarker analysis. Currently, various normalization techniques such as creatinine ratio, osmolality, specific gravity, dry mass, urine volume, and area under the curve are used to account for the renal dilution. However, these normalization techniques have their...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 3, 2019 Category: Drugs & Pharmacology Source Type: research

UPLC-MS/MS method for the determination of talazoparib in rat plasma and its pharmacokinetic study
Publication date: Available online 31 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lei Ye, Jingjing Chen, Shuang-long Li, Yong-liang Zhu, Saili Xie, Xiaoxiang DuAbstractIn the present study, an accurate and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of plasma talazoparib concentration in rats was developed and established. The purpose of chromatographic separation of talazoparib and the internal standard (bosutinib) was achieved on an Acquity BEH C18 (2.1 mm × 50 mm, 1.7 μm...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 2, 2019 Category: Drugs & Pharmacology Source Type: research

In vitro selection of tacrolimus binding aptamer by systematic evolution of ligands by exponential enrichment method for the development of a fluorescent aptasensor for sensitive detection of tacrolimus
In this study, specific aptamers (Apt122 and Apt125) for TAC were isolated via systematic evolution of ligands by exponential enrichment method using magnetic beads to immobilize the target. After determination of binding constants of aptamers by flow cytometry analysis, Apt122 was selected and labeled with ATTO 647 N as a fluorophore to develop a fluorescent sensing platform for detection of TAC using graphene oxide (GO) as a fluorescence quencher. The designed aptasensor could detect TAC in phosphate buffer saline (10 mM PBS) and serum samples with detection limits as low as 1.4 and 2.5 nM, respectiv...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 2, 2019 Category: Drugs & Pharmacology Source Type: research

Discovery of metabolite profiles of metabolic syndrome using untargeted and targeted LC–MS based lipidomics approach
Publication date: 5 January 2020Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 177Author(s): Li-li Gong, Song Yang, Wen Zhang, Fei-fei Han, Ya-li Lv, Ling-ling Xuan, He Liu, Li-hong LiuAbstractMetabolic syndrome (MetS) is an important risk factor for type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. Biomarkers can provide insight into the mechanism, facilitate early detection, and monitor progression of MetS and its response to therapeutic interventions. To identify potential biomarkers, we applied a non-targeted and targeted lipidomics method to characterize plasma metaboli...
Source: Journal of Pharmaceutical and Biomedical Analysis - September 2, 2019 Category: Drugs & Pharmacology Source Type: research

Quantifying Protein Aggregation Kinetics using Electrospray Differential Mobility Analysis
In this study, an aerosol-based technique, electrospray differential mobility analysis (ES-DMA), was used to quantify thermally-induced protein aggregation kinetics for bovine serum albumin (BSA) and α-chymotrypsinogen A (α-chymo), employing a new methodology to modify the solution for compatibility with the electrospray process. Results are compared orthogonally with asymmetrical-flow field-flow fractionation (AF4), a hydrodynamic separation technique with UV detection. Measurements were conducted over a range of protein concentrations and temperatures. Both techniques successfully resolved the protein monomer...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 31, 2019 Category: Drugs & Pharmacology Source Type: research

Recent advances in untargeted and targeted approaches applied in herbal-extracts and essential-oils fingerprinting - A review
Publication date: Available online 30 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mourad Kharbach, Ilias Marmouzi, Meryem El Jemli, Abdelaziz Bouklouze, Yvan Vander HeydenAbstractHerbal extracts and essential oils have been used over the centuries for their dietary, cosmetic and therapeutic properties. Quality control is needed to guarantee the safety and quality of these consumables. In this regard, fingerprinting techniques are important for inspection of the authenticity and for quality control. Analytical fingerprinting techniques provide signals related to the composition of a matrix...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 31, 2019 Category: Drugs & Pharmacology Source Type: research

Binding and mucoadhesion of sulfurated derivatives of quaternary ammonium-chitosans and their nanoaggregates: an NMR investigation
Publication date: Available online 30 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Andrea Cesari, Angela Fabiano, Anna Maria Piras, Ylenia Zambito, Gloria Uccello-Barretta, Federica BalzanoAbstractThe effect of insertion of SH and S-protected groups on the binding and mucoadhesion properties of quaternary ammonium-chitosans and their nanoparticulate forms has been investigated by NMR spectroscopy. Diclofenac sodium salt has been assumed as low molecular weight probe to detect the different binding behaviour of polymeric materials; mucin from bovine submaxillary glands was selected as the m...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 31, 2019 Category: Drugs & Pharmacology Source Type: research

Quo Vadis qNMR?
Publication date: Available online 30 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bernd Diehl, Ulrike Holzgrabe, Yulia Monakhova, Torsten SchönbergerAbstractThe quantification of a drug, its impurities, and e.g. components of a mixture has become routine in NMR laboratories and many applications have been described in the literature. However, besides simply using 1D 1H or 13C NMR, a number of more advanced methods has been developed and used in the past. Here, we want to describe the applicability of nuclei beyond the classical ones 1H and 13C. Mixtures can be characterized much bett...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 31, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of chiral and achiral impurities of ivabradine on a cellulose tris(3-chloro-4-methylphenylcarbamate) chiral column using polar organic mode
Publication date: Available online 30 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Elek Ferencz, Béla Kovács, Francisc Boda, Mohammadhassan Foroughbakhshfasaei, Éva Katalin Kelemen, Gergő Tóth, Zoltán-István SzabóAbstractA high performance liquid chromatographic method was developed for the simultaneous determination of the related substances (R-ivabradine, dehydro-S-ivabradine, N-demethyl-S-ivabradine, ((S)-3,4-dimethoxy-bicyclo[4.2.0]octa-1,3,5-triene-7-yl-methyl)-methyl-amine) and 1-(7,8-dimethoxy-1,3,4,5-tetrahydro-2H-3-benzazepine-2-on...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 31, 2019 Category: Drugs & Pharmacology Source Type: research

Discovery of Metabolite Profiles of Metabolic Syndrome Using Untargeted and Targeted LC-MS based Lipidomics Approach
Publication date: Available online 29 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Li-li Gong, Song Yang, Wen Zhang, Fei-fei Han, Ya-li Lv, Ling-ling Xuan, He Liu, Li-hong LiuAbstractMetabolic syndrome (MetS) is an important risk factor for type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. Biomarkers can provide insight into the mechanism, facilitate early detection, and monitor progression of MetS and its response to therapeutic interventions. To identify potential biomarkers, we applied a non-targeted and targeted lipidomics method to characterize plasma met...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 30, 2019 Category: Drugs & Pharmacology Source Type: research