Isotope Dilution LC-Orbitrap-HRMS with Automated Sample Preparation for the Simultaneous Quantification of 11 Antimycotics in Human Serum
ConclusionHRMS was shown to be suitable for TDM of small molecules when compared to tandem mass spectrometry. The novel HRMS method is quickly installed and may be a robust and reliable tool for routine TDM of antimycotics in clinical laboratories. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of the species status of Fallopia multiflora, Fallopia multiflora var. angulata and Fallopia multiflora var. ciliinervis based on morphology, molecular phylogeny, and chemical analysis
Publication date: Available online 25 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hui-qun Xie, Shan-shan Chu, Liang-ping Zha, Ming-en Cheng, Lu Jiang, Dan-dan Ren, Yi Yu, Hua-sheng Peng, Dai-yin PengAbstractRelationships among Fallopia multiflora (Thunb.) Haraldson., F. multiflora var. angulata (S. Y. Liu) H. J. Yan, Z. J. Fang & Shi Xiao Yu., and F. multiflora var. ciliinervis (Nakai) Yonekura & H. Ohashi. were determined based on macroscopic and microscopic morphology, molecular phylogeny, and chemical analysis. The macroscopic and microscopic morphologies of root tubers or rhi...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 26, 2019 Category: Drugs & Pharmacology Source Type: research

Electrospun nanofiber-based niflumic acid capsules with superior physicochemical properties
In this study, both the amount of the dissolved active ingredient and the dissolution kinetics has been improved significantly when the nanofibrous mats were used in the drug formulation. A 15-fold increase in the dissolved amount of the produced amorphous niflumic acid nanofiber was observed compared to the dissolved amount of the raw drug within the first 15 minutes. Capsule formulation was made by mixing the electrospun nanofibers with a microcrystalline cellulose filler agent. When comparing the dissolution rate of the capsule formulation on the market with the nanofibrous capsules, a 14-fold increase was observ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 24, 2019 Category: Drugs & Pharmacology Source Type: research

Identification and determination of bioactive phenylpropanoid glycosides of Aloysia polystachya (Griseb. et Moldenke) by HPLC-MS
Publication date: Available online 22 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lucia Marchetti, Federica Pellati, Riccardo Graziosi, Virginia Brighenti, Diego Pinetti, Davide BertelliAbstractAloysia polystachya (Griseb. et Moldenke) has not been deeply investigated in past years and currently data about its chemical composition are limited. Phenolic compounds characterization can be very difficult in vegetable matrix, owing to bonds to sugar moieties or conjugation, giving rise to complex structures. In this work, methanolic extracts of Aloysia polystachya leaves were analyzed by HPLC...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 23, 2019 Category: Drugs & Pharmacology Source Type: research

Analytical quality by design: development and control strategy for a LC method to evaluate the cannabinoids content in cannabis olive oil extracts
In this study, a fast RP-HPLC/UV method has been developed to quantify CBD and Δ9-THC in cannabis olive oil extracts. The analytical quality by design strategy has been applied to the method development, setting critical resolution and total analysis time as critical method attributes (CMAs), and selecting column temperature, buffer pH and flow rate as critical method parameters. Information from Doehlert Design in response surface methodology combined to Monte-Carlo simulations led to draw the risk of failure maps and to identify the method operable design region. The method was validated according to the Internatio...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 22, 2019 Category: Drugs & Pharmacology Source Type: research

High-performance countercurrent chromatographic isolation of acylated iridoid diglycosides from Verbascum ovalifolium Donn ex Sims and evaluation of their inhibitory potential on IL-8 and TNF-α production
Publication date: Available online 18 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Simon Vlad Luca, Monika Ewa Czerwińska, Anca Miron, Ana Clara Aprotosoaie, Laurence Marcourt, Jean-Luc Wolfender, Sebastian Granica, Krystyna Skalicka-WoźniakAbstractIn traditional folk medicine, Verbascum species have been used since ancient times to treat respiratory disorders, hemorrhoids, diarrhea, wounds, eczema and other types of inflammatory skin conditions. Despite the fact that more than 200 bioactive constituents (phenylethanoids, flavonoids, neolignan glycosides, phenolic acids, iridoids, sapon...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 20, 2019 Category: Drugs & Pharmacology Source Type: research

HPLC-MS/MS analysis of ilimaquinone and its application in a pharmacokinetic study in rats
Publication date: Available online 17 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Heebin Son, Keumhan Noh, Changhyun Kang, MinKyun Na, Sangtaek Oh, Im-Sook Song, Wonku KangAbstractIlimaquinone, a metabolite isolated from the marine sponge Hippiospongia metachromia, has antimicrobial, cytotoxic, anti-HIV, anti-inflammatory, and anti-cancer activities. A new quantitative analytical method for determination of ilimaquinone in rat plasma using HPLC-MS/MS was developed and validated. Ascorbic acid was added to ensure the stability of ilimaquinone in plasma. After protein precipitation using a...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 17, 2019 Category: Drugs & Pharmacology Source Type: research

Comparison between counterfeit and authentic medicines: a novel approach using Differential Scanning Calorimetry and Hierarchical Cluster Analysis
In this study, thermal analysis of 25 counterfeit Viagra and Cialis tablets seized by Brazilian Federal Police were performed by DSC and compared to their authentic medicines and analytical standards, along with chemometric tools. Authentic samples of Viagra displayed a similar thermal profile with the API, while Cialis were different with additional endothermic peaks, that could be related to excipients interference. Thermograms of Viagra counterfeit samples were similar to authentic samples, while Cialis showed an enlargement and displacement of endothermic peaks. Also, some Cialis counterfeit samples showed melting peak...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 17, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of unstable degradation impurities of a benzodiazepine and their quantification without isolation using multiple linear regression
Publication date: Available online 17 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): John M. Campbell, Kaitie Grinias, Kevin Facchine, Benoît Igne, Jacalyn Clawson, John Peterson, Andy Wolters, Jeremy Barry, Simon Watson, Kevin LeachAbstractThis manuscript presents a novel methodology for calculating the relative response factors (RRFs) of unstable degradation impurities of molibresib (1). The degradation impurities were observed by HPLC during stress testing and were accompanied by large mass balance deficits. However, the impurities could not be isolated for traditional RRF determin...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 17, 2019 Category: Drugs & Pharmacology Source Type: research

Sub-picomolar quantification of PTH 1-34 in plasma by UHPLC-MS/MS after subcutaneous injection of teriparatide and identification of PTH 1-33, its degradation product
Publication date: 20 March 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 166Author(s): Philippe J. Eugster, Haithem Chtioui, Adeline Herren, Marielle Dunand, Delphine Cappelle, Julien Bourquin, Thierry Buclin, Eric GrouzmannAbstractTeriparatide (PTH 1-34, Forsteo®) is a bioactive N-terminal fragment of the native endogenous parathyroid hormone (PTH 1-84) recommended for the treatment of osteoporosis in patients with high risk of fracture. Since PTH 1-34 may undergo proteolysis we have validated an ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for unambig...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 17, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of residues and metabolites of more than 140 pharmacologically active substances in meat by liquid chromatography coupled to high resolution Orbitrap mass spectrometry
This study reports an analytical method for simultaneous identification, screening and quantification of 164 residues and metabolites of pharmacologically active substances belonging to such therapeutic classes as anti-infectious (antibiotics and chemotheurapeutics), anti-inflammatory and antiparasitic agents (against protozoa, endo- and ectoparasites), corticoids and agents acting on the nervous and reproductive systems, substances with hormonal and thyreostatic action, and beta agonists. Different sample preparation procedures were compared and optimised for the detection of selected veterinary drugs in chicken, porcine ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Development and Validation of a UPLC-MS/MS Method for Determination of Motesanib in Plasma: Application to Metabolic Stability and Pharmacokinetic Studies in Rats
Publication date: Available online 14 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Essam Ezzeldin, Muzaffar Iqbal, Rashad Al-Salahi, Toqa El-NahhasAbstractMotesanib is a potent angiokinase inhibitor, has shown potential therapeutic effects against various cancers. An accurate, reproducible, rapid, specific, sensitive, and valid ultraperformance liquid chromatography-tandem mass spectrometry method was established to quantify motesanib in rat plasma. Motesanib and linifanib (used as an internal standard; IS) were extracted from plasma by liquid-liquid extraction using tert-butyl methyl eth...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Identification of novel serum biomarker for the detection of acute myeloid leukemia based on liquid chromatography-mass spectrometry
Publication date: Available online 14 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Dong Wang, Guangguo Tan, Haibo Wang, Peng Chen, Jie Hao, Yanyu WangAbstractAcute myeloid leukemia (AML) is a life-threatening hematological malignancy. Traditional diagnosis of AML depends on invasive bone marrow biopsies. To recognize the metabolic characteristics related with AML and search for early non-invasive biomarkers of AML, in this work we applied ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOFMS)-based metabolomoc method to profile seru...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid characterization of the absorbed constituents in rat serum after oral administration and action mechanism of Naozhenning granule using LC-MS and network pharmacology
In this study, the ultrahigh performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry was applied to identify the absorbed constituents of Naozhenning in the rat serum. A total of 60 compounds, including 30 prototype components and 30 metabolites were identified. Then the absorbed constituents were subjected to the network pharmacology analysis. The compound-target-disease (CTD) and KEGG pathway analysis revealed that 40 absorbed constituents, 56 target genes and three key pathways such as RAS, PI3K/Akt, MAPK, TGFβ were probably related with the efficacy of Naozhenning against cerebr...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Characterization of tolazamide binding with glycated and normal human serum albumin by using high-performance affinity chromatography
Publication date: Available online 14 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Pingyang Tao, Zhao Li, Ashley G. Woolfork, David S. HageAbstractSulfonylurea drugs are antidiabetic drugs that are utilized in the treatment of type II diabetes and often have significant binding with human serum albumin (HSA). Immobilized samples of normal or glycated HSA in affinity microcolumns were used to investigate interactions of these proteins with the sulfonylurea drug tolazamide. HPLC and frontal analysis were used to first examine the overall binding of this drug with these samples of HSA. It wa...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Ultrasound-assisted emulsification liquid phase microextraction method based on deep eutectic solvent as extraction solvent for determination of five pesticides in traditional Chinese medicine
In this study, a method was developed for determination of five pesticide residues including triadimenol, fipronil, tebuconazole, hexaconazole and diniconazole in five different traditional Chinese medicines (TCMs). In the developed method, DES was selected as extraction solvent, ultrasound-assisted emulsification liquid phase microextraction (UA-ELPME) was used for extraction and high performance liquid chromatography (HPLC) equipped with a diode array detector (DAD) was used for quantification. In this method, DES was synthesized with choline chloride and phenol at the molar ratio 1:4 using the heating and stirring metho...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

A metabolomic study based on accurate mass and isotopic fine structures by dual mode combined-FT-ICR-MS to explore the effects of Rhodiola crenulata extract on Alzheimer disease in rats
Publication date: Available online 14 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaoxue Zhang, Xiwei Jiang, Xue Wang, Yangyang Zhao, Lianqun Jia, Fen Chen, Ran Yin, Fei HanAbstractA metabolomic strategy based on accurate mass and isotopic fine structures (IFSs) by dual mode combined-Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was established to explore the effects of Rhodiola crenulata extract (RCE) on Alzheimer disease (AD) in rats. Experimental AD model was induced in rats by bilateral hippocampal injection of Aβ1-42, and Morris water maze task (MWM) ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolomics profiling provides valuable insights into the underlying mechanisms of Morinda officinalis on protecting glucocorticoid-induced osteoporosis
Publication date: Available online 14 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tianshuang Xia, Xin Dong, Liuyue Lin, Yiping Jiang, Xueqin Ma, Hailiang Xin, Qiaoyan Zhang, Luping QinAbstractMorinda officinalis (MO) has long been used as a traditional herbal medicine for the treatment of bone fractures and joint diseases in China. Monotropein (Mon) and rubiadin-1-methyl ether (Rub) are major bioactive components in MO. Ample evidence shows that MO and its chemical constituents can prevent osteoporosis induced by estrogen-deficiency and ageing. However, there is no study reporting glucoc...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Monolith weak affinity chromatography for μg-protein-ligand interaction study
Publication date: 20 March 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 166Author(s): Lucile Lecas, Jérôme Randon, Alain Berthod, Vincent Dugas, Claire DemesmayAbstractAffinity monolith columns of 375 nL (effective length 8.5 cm, internal diameter 75 μm) were developed for protein-ligand affinity investigations needing only 3 μg of human serum albumin (HSA). To promote specific interactions and avoid non-specific ones, different combinations of monolithic supports and bio-functionalization pathways were evaluated. Silica and glycidylmethacrylate based ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 15, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid, hydrolysis-free, dilute-and-shoot method for the determination of buprenorphine, norbuprenorphine and their glucuronides in urine samples using UHPLC-MS/MS
Publication date: Available online 11 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Marco Agostini, Caterina Renzoni, Elisabetta Pierini, Maurizio Piergiovanni, Veronica Termopoli, Giorgio Famiglini, Pierangela Palma, Achille CappielloAbstractBuprenorphine and its metabolites are routinely monitored to assess patient compliance with drug detoxification programs or as pain killers. A rapid method for the simultaneous analysis of buprenorphine, norbuprenorphine, and glucuronides in urine using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2019 Category: Drugs & Pharmacology Source Type: research

The Effect of Fatty Acids in Red Blood Cell Membranes on the Dynamics of Inflammatory Markers Following the Coronary Stent Implantation
Publication date: Available online 11 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Vladimíra Mužáková, Milan Meloun, Andrea Jindrová, Alexander ČeganAbstractThe effect of 20 fatty acids in erythrocyte cell membranes on the extent of inflammatory response and cell oxidative stress was evaluated using multidimensional statistical data analysis in 54 patients suffering from ischemic heart disease undergoing percutaneous coronary intervention with coronary stent implantation using multidimensional statistical data analysis. A systemic inflammatory response was in...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of total and unbound ribociclib in human plasma and brain tumor tissues using liquid chromatography coupled with tandem mass spectrometry
This study was to develop and validate a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for determining total and unbound concentrations of ribociclib in human plasma and brain tumor tissue samples. Plasma and tumor homogenate samples were extracted using protein precipitation with acetonitrile. Unbound fraction in plasma or tumor homogenate was determined by equilibrium dialysis method. Chromatographic separation was achieved based on aqueous normal-phase chromatography mechanism on a Waters XBridgeTM Amide column under isocratic elution with acetonitrile-ammonium formate (10 mM, pH 3) (75:25...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 12, 2019 Category: Drugs & Pharmacology Source Type: research

A strategy based on fingerprinting and chemometrics for the detection of regulated plants in plant food supplements from the Belgian market: two case studies
Publication date: Available online 11 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Deconinck E., Vanhamme M., Bothy J.L., P. CourselleAbstractThe sale and consumption of plant food supplements is increasing, especially in the western world. A lot of these supplements can be bought through internet, where a lot of illegal trade is going on. Every year seized dietary supplements are send to laboratories in order to screen for the presence of chemical adulterants or illegally added active pharmaceutical ingredients, though also herbal adulteration occurs and is given less attention.In this p...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 11, 2019 Category: Drugs & Pharmacology Source Type: research

A systematic review of the stability of finished pharmaceutical products and drug substances beyond their labeled expiry dates
Publication date: Available online 11 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Markus Zilker, Fritz Sörgel, Ulrike HolzgrabeAbstractIn recent years, there has been a very active debate about the stability of drug products especially after exceeding the expiry dates. The regulatory authorities require comprehensive stability data for market approval. The shelf-life obtained determines the expiry date, which is typically between 1 and 5 years and commonly set in a conservative manner. Conducting stability studies is a resource- and time-consuming matter for the pharmaceutical manuf...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 11, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 11, 2019 Category: Drugs & Pharmacology Source Type: research

A validated UPLC-MS/MS method for pharmacokinetic study of Inflachromene, a novel microglia inhibitor
Publication date: Available online 9 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hwan Hee Lee, So Hee Im, Sunjoo Ahn, Myung Ae Bae, Seung Bum Park, Sang Kyum Kim, Jin Sook SongAbstractInflachromene (ICM), a novel microglia inhibitor, is under development to treat neuroinflammatory disorders. For the pharmacokinetic evaluation of ICM, we developed quantitative ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method in rat plasma. Protein precipitation method with acetonitrile (ACN) was used for plasma sample preparation. The analyte and carbamazepin...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2019 Category: Drugs & Pharmacology Source Type: research

Qualitative analysis of the roots of salvia miltiorrhiza and Salvia yunnanensis based on NIR, UHPLC and LC-MS-MS
Publication date: Available online 9 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lijun Ni, Fangfang Zhang, Mingyue Han, Liguo Zhang, Shaorong Luan, Wei Li, Haixing Deng, Zhuhui Lan, Zhongxiang Wu, Xiaofang Luo, Leslaw MleczkoAbstractNear infrared spectroscopy (NIR) was applied to discriminate the roots of salvia miltiorhiza Bunge (Danshen for short) and salvia yunnanensis C. H. Wright (Zidanshen for short) by means of principal component analysis (PCA), improved and simplified K nearest neighbors (IS-KNN). Furthermore, an ultra-high performance liquid chromatographic (UHPLC) coupled with...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2019 Category: Drugs & Pharmacology Source Type: research

Monolith weak affinity chromatography for µg-protein-ligand interaction study
Publication date: Available online 9 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lucile Lecas, Jérôme Randon, Alain Berthod, Vincent Dugas, Claire DemesmayAbstractAffinity monolith columns of 375 nL (effective length 8.5 cm, internal diameter 75 µm) were developed for protein-ligand affinity investigations needing only 3 µg of human serum albumin (HSA). To promote specific interactions and avoid non-specific ones, different combinations of monolithic supports and bio-functionalization pathways were evaluated. Silica and glycidylmethacr...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2019 Category: Drugs & Pharmacology Source Type: research

Sub-picomolar quantification of PTH1-34 in plasma by UHPLC-MS/MS after subcutaneous injection of teriparatide and identification of PTH1-33, its degradation product
Publication date: Available online 9 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Philippe J. Eugster, Haithem Chtioui, Adeline Herren, Marielle Dunand, Delphine Cappelle, Julien Bourquin, Thierry Buclin, Eric GrouzmannAbstractTeriparatide (PTH1-34, Forsteo®) is a bioactive N-terminal fragment of the native endogenous parathyroid hormone (PTH1-84) recommended for the treatment of osteoporosis in patients with high risk of fracture. Since PTH1-34 may undergo proteolysis we have validated an ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for unam...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2019 Category: Drugs & Pharmacology Source Type: research

Bioassay based screening for the antiplatelet aggregation quality markers of Polygonum multiflorum with UPLC and chemometrics
Publication date: Available online 9 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Chunyu Li, Can Tu, Yi Che, Mingyu Zhang, Bin Dong, Xiaowei Zhou, Yafei Shi, Guohui Li, Jiabo WangAbstractCurrently, an increasing number of human’s health is seriously affected by acute thrombotic events. In China, Polygonum multiflorum (PM) is commonly used to treat diseases associated with thrombosis. Our previous work showed that PM could inhibit platelet aggregation that play a key role in the pathogenesis of thrombosis. However, the constituents of PM are complicated and its quality control method...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2019 Category: Drugs & Pharmacology Source Type: research

Stability behaviour of antiretroviral drugs and their combinations. 9: Identification of incompatible excipients
Publication date: Available online 8 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Moolchand Kurmi, Archana Sahu, Mayurbhai Kathadbhai Ladumor, Arvind Kumar Bansal, Saranjit SinghAbstractIncompatibility studies of antiretroviral drugs, viz., lamivudine (3TC), emtricitabine (FTC), abacavir sulfate (ABC), tenofovir disoproxil fumarate (TDF), zidovudine (ZDV), efavirenz (EFV) and nevirapine (NVP) were carried out in the presence of ten selected excipients, i.e., microcrystalline cellulose, lactose monohydrate, starch, magnesium stearate, sodium lauryl sulfate, sodium starch glycolate, croscar...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a UPLC-ESI-MS method for simultaneous determination of flavonoids and diterpenes in Egletes viscosa (L.) Less herbal products
Publication date: Available online 8 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kaline Rodrigues Carvalho, Guilherme Julião Zocolo, Rita de Cássia Alves Pereira, Fátima Itana Chaves Custódio Martins, Paulo Riceli Vasconcelos Ribeiro, Edy Sousa de Brito, Edilberto Rocha Silveira, Kirley Marques CanutoAbstractEgletes viscosa (L.) Less (Asteraceae) is a tropical aromatic herb whose flower buds are used for gastrointestinal disorders. Its gastroprotective properties have been attributed to the flavonoid ternatin and the furan diterpenes tanabalin and centipedic a...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2019 Category: Drugs & Pharmacology Source Type: research

Corrigendum to “Quantification of diterpene acids in Copaiba oleoresin by UHPLC-ELSD and heteronuclear two-dimensional qNMR” [J. Pharm. Biomed. Anal. 160 (2018) 126–134]
Publication date: Available online 8 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Serhat Sezai Çiçek, Anna Laís Pfeifer Barbosa, Ulrich Girreser (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - January 9, 2019 Category: Drugs & Pharmacology Source Type: research

Investigations into the therapeutic effects of aerial and stem parts of Buxus papillosa C.K. Schneid.: In vitro chemical, biological and toxicological perspectives
In this study, different solvent extracts (methanol, dichloromethane and n-hexane) from aerial and stem parts of Buxus papillosa C.K. Schneid (Buxaceae) were investigated for a panoply of bioassays. Biological profiles were established by determining antioxidant and enzyme inhibition profiles. Toxicity was tested using MTT cell viability assay on five different human cancer cell lines i.e, MCF-7, MDA-MB-231 CaSki, DU-145 and SW-480. For chemical fingerprinting, total bioactive contents and UHPLC-MS secondary metabolites profile were determined. Generally, both aerial and stem methanol extracts had highest total bioactive c...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 8, 2019 Category: Drugs & Pharmacology Source Type: research

Isolation and structural characterization of degradation products of afatinib dimaleate by LC-Q-TOF/MS/MS and NMR: cytotoxicity evaluation of afatinib and isolated degradation products
Publication date: Available online 7 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Balasaheb B. Chavan, Sawant Vitthal, Roshan M. Borkar, R. Srinivas, M.V.N. Kumar TalluriAbstractAfatinib is an irreversible tyrosine kinase receptor inhibitor which was approved lately by USFDA for the treatment of metastatic non-small cell lung cancer (NSCLC). AFT was subjected to stress degradation studies under hydrolytic (acid, base and neutral), oxidative, thermal and photolytic conditions to investigate the inherent stability of the drug. The present study describes the simple and rapid HPLC method dev...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 8, 2019 Category: Drugs & Pharmacology Source Type: research

Enantioseparation of N-acetyl-dl-cysteine as o-phtaldialdehyde derivatives obtained with various primary aliphatic amine additives on polysaccharide-based chiral stationary phases
Publication date: Available online 8 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Michal DoušaAbstractA sensitive and rapid high-performance liquid chromatography (HPLC) method was developed to enantioseparation of N-acetyl-DL-cysteine after precolumn derivatization using o-phthaldialdehyde and primary aliphatic amines. Seven polysaccharide-based chiral columns were tested in a reversed phase mode. Under the optimal chromatographic conditions, N-acetyl-DL-cysteine derivatives were completely enantioseparated on Chiralcel OZ-3R column with the resolution more than 2.5. The impact of...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 8, 2019 Category: Drugs & Pharmacology Source Type: research

Solid-phase extraction combined with dispersive liquid-liquid microextraction/HPLC-UV as a sensitive and efficient method for extraction, pre-concentration and simultaneous determination of antiretroviral drugs nevirapine, efavirenz and nelfinavir in pharmaceutical formulations and biological samples
Publication date: Available online 4 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mehregan Safari, Mojtaba Shamsipur, Parvin Zohrabi, Homeira EbrahimzadehAbstractTherapeutic drug monitoring (TDM) of antiretroviral drugs requires accurate and precise analysis of their trace amounts in plasma samples. Solid-phase extraction (SPE) coupled with dispersive liquid-liquid microextraction based on solidification of floating organic drop (DLLME-SFO) was introduced as a simple and sensitive method for extraction, pre-concentration and simultaneous determination of efavirenz (EFV), nelfinavir (NFV) ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 5, 2019 Category: Drugs & Pharmacology Source Type: research

Hydrophilic-interaction planar chromatography in ultra-sensitive determination of α-aminocephalosporin antibiotics. Application to analysis of cefalexin in goat milk samples using modified QuEChERS extraction technique
Publication date: Available online 3 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Azza H. Rageh, Sherien A. Abdel-Rahim, Hassan F. Askal, Gamal A. SalehAbstractA highly sensitive, selective and precise HPTLC method coupled with fluorescence detection was developed and validated for the determination of α-aminocephalosporin antibiotics; namely cefalexin, cefadroxil and cefradine in their standard solutions. The applicability of the developed methodology was demonstrated via analysis of cefalexin in goat milk samples. Full optimization of the fluorescence derivatization reaction was c...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 4, 2019 Category: Drugs & Pharmacology Source Type: research

Hypercrosslinked strong anion-exchange polymers for selective extraction of fluoroquinolones in milk samples
Publication date: Available online 31 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaoping Liang, Ping Hu, Hongyang Zhang, Wen TanAbstractIn this work, hypercrosslinked strong anion-exchange polymer resins (HXLPP-SAX) were synthesized and evaluated as solid-phase extraction (SPE) sorbents for extracting fluoroquinolones (FQs) in milk samples. These particles were prepared using a protocol that was facile and cost-effective by combining hypercrosslinking reactions and quaternisation reactions in one step. After the synthesis, the morphological properties and anion-exchange, adsorption, a...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 1, 2019 Category: Drugs & Pharmacology Source Type: research

Isolation of punicalagin from Punica granatum rind extract using mass-directed semi-preparative ESI-AP single quadrupole LC-MS
Publication date: Available online 31 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mirko Barbieri, Charles M. HeardAbstractWe are living in a era of alarming increases in microbial resistance to currently available antibiotics, and there is a growing need for new pharmaceutical products to treat infectious diseases. The pomegranate is an edible fruit that has virucidal and antimicrobial activities which is primarily attributable to the high concentration of hydrolysable tannins. Punicalagin, a high molecular weight tannin (1084.7), accounts for approximately 70% of the total and is conce...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 1, 2019 Category: Drugs & Pharmacology Source Type: research

Micro-solid phase extraction and LC–MS3 for the determination of triptorelin in rat plasma and application to a pharmacokinetic study
Publication date: 20 March 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 166Author(s): Tianming Ren, Zhi Zhang, John Paul Fawcett, Dong Sun, Jingkai GuAbstractTriptorelin is a synthetic decapeptide used for the treatment of prostate cancer. Attempts to determine triptorelin in clinical use by liquid chromatography-tandem mass spectrometry with multiple reaction monitoring have encountered problems due to its low concentration in plasma (pg/mL) and interference from endogenous peptides. We have overcome these issues using micro-solid phase extraction (μ-SPE) on Oasis® HLB 96-well μElution p...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 1, 2019 Category: Drugs & Pharmacology Source Type: research

A high-performance liquid chromatography-tandem mass spectrometry method for the determination of lifrafenib, a novel RAF kinase and EGFR inhibitor, in human plasma and urine and its application in clinical pharmacokinetic study
Publication date: 20 March 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 166Author(s): Xueting Yao, Ling Song, Yang Liu, Huanhuan Wang, Jie Liu, Ji Jiang, Dongyang Liu, Pei HuAbstractLifirafenib (BGB-283), a dual inhibitor trageting BRAF kinase and EGFR, showed favorable efficacy and safety in treating patients with different cancer types harboring mutations in BRAF, KRAS and NRAS. In order to support the clinical pharmacokinetic study, a sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) method was developed and validated to quantify lifirafenib concentrati...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 1, 2019 Category: Drugs & Pharmacology Source Type: research

Study on the herb-herb interaction of Danqi Tongmai Tablet based on the pharmacokinetics of twelve notoginsenoides in acute myocardial ischemia and sham rats
Publication date: Available online 29 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Haijuan Zhi, Yanping Deng, Bingpeng Yan, Zhenwei Li, Sumei Han, Yibei Zhang, Jinjun Hou, Wu Wanying, Dean GuoAbstractDanqi Tongmai tablet (DQTT), an innovative TCM formula under clinical trials, is composed of salvianolic acids (SA) and panax notoginsenosides (PNE) for the treatment of coronary heart disease and angina pectoris. However, the in vivo herb-herb interaction of DQTT remains unclear. In the present research, a rapid, reliable and sensitive method for quantitative analysis of multi-notoginsenosi...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 30, 2018 Category: Drugs & Pharmacology Source Type: research

LC-MS application for Therapeutic Drug Monitoring in alternative matrices
Publication date: Available online 29 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Valeria Avataneo, Antonio D’Avolio, Jessica Cusato, Marco Cantù, Amedeo De NicolòAbstractNowadays the practice of therapeutic drug monitoring, consisting in the measurement of drugs concentrations in biological matrices in order to guide possible posological adjustments, is becoming more and more important for the management and optimization of several treatments, especially when drugs with narrow therapeutic indexes are administered.Although TDM on plasma samples is currently considere...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 30, 2018 Category: Drugs & Pharmacology Source Type: research

Development and validation of a sensitive method for alkyl sulfonate genotoxic impurities determination in drug substances using gas chromatography coupled to triple quadrupole mass spectrometry
Publication date: Available online 30 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhen Liu, Huajun Fan, Yihui Zhou, Xiaowei Qian, Jihui Tu, Bin Chen, Gengli DuanAbstractAlkyl sulfonate esters have been widely concerned as genotoxic impurities (GTIs). A gas chromatography triple-quadrupole mass spectrometry (GC-MS/MS) method has been developed for trace determination of 9 commonly encountered sulfonate esters in drug substances. Three different solvents of acetonitrile (ACN), dichloromethane (DCM) and ethyl acetate (EtOAc) were evaluated as diluents to accommodate different solubilities ...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 30, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative bioanalytical assay for the human epidermal growth factor receptor (HER) inhibitor dacomitinib in rat plasma by UPLC-MS/MS
Publication date: Available online 28 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiangjun Qiu, Qianmeng Lin, Zongdi Ning, Xin Qian, Pengbo Li, Lei Ye, Saili XieAbstractDacomitinib is a highly selective irreversible small-molecule inhibitor of the human epidermal growth factor receptor (HER) family of tyrosine kinases. A simple and quick bioanalytical method was completely developed and validated for the assay and pharmacokinetic investigation of dacomitinib in rat plasma using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Proteins in 0.1 mL plasm...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 29, 2018 Category: Drugs & Pharmacology Source Type: research

Predictive metabolic signatures for the occurrence and development of diabetic nephropathy and the intervention of Ginkgo biloba leaves extract based on gas or liquid chromatography with mass spectrometry
Publication date: Available online 26 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yan Du, Bing-ju Xu, Xu Deng, Xiao-wen Wu, Yin-jie Li, Shi-rui Wang, Yi-nan Wang, Shuai Ji, Meng-zhe Guo, Dong-zhi Yang, Dao-quan TangAbstractDiabetic nephropathy (DN) is one of the leading causes of death in diabetes mellitus (DM). Early warning and therapy has significant clinical value for DN. This research sought to find biomarkers to predict the occurrence and development of DN and the intervention of Ginkgo biloba leaves extract (GBE) by quantifying fatty acids, amino acids, and nucleosides and nucleo...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 27, 2018 Category: Drugs & Pharmacology Source Type: research

Micro-solid phase extraction and LC-MS3 for the determination of triptorelin in rat plasma and application to a pharmacokinetic study
Publication date: Available online 26 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tianming Ren, Zhi Zhang, John Paul Fawcett, Dong Sun, Jingkai GuAbstractTriptorelin is a synthetic decapeptide used for the treatment of prostate cancer. Attempts to determine triptorelin in clinical use by liquid chromatography-tandem mass spectrometry with multiple reaction monitoring have encountered problems due to its low concentration in plasma (pg/mL) and interference from endogenous peptides. We have overcome these issues using micro-solid phase extraction (µ-SPE) on Oasis® HLB 96-well &m...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 27, 2018 Category: Drugs & Pharmacology Source Type: research

Two distinct conformers of SAG investigated by solution NMR and variable-temperature experiments
Publication date: Available online 12 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Haipeng Jiang, Xian Wang, Xiaoliang CuiAbstractSAG (Smo agonist) is high specific agonist of Smo receptor, it is widely used as chemical probe to explore the therapeutic value of activating the Hh-signaling pathway. In the present work, the coexistence of two distinct conformers (A and B) of SAG are revealed by solution NMR, the 3D structural difference of the two conformers are elucidated by ROESY spectroscopy and MMFF94 program. It is discovered that both conformers are agonists of Smo receptor, and the ...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 25, 2018 Category: Drugs & Pharmacology Source Type: research

Effect of Wuziyanzong pill on metabolism of dapoxetine in vivo and in vitro
Publication date: Available online 15 December 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hong-chang Yuan, Zhi-jian Deng, Xin-min Liu, Ting-fang Dong, Xiang-jun Qiu, Zeng NanAbstractIn vitro incubation of rat liver microsomes with 30 µL of 100 μmol L-1 dapoxetine and 30 µL of 10, 100, 250, 500, 1,000, 2,500, or 5,000 μg mL-1 Wuziyanzong pill was performed at 37℃ for 60 min. Dapoxetine concentration was analyzed by high performance liquid chromatography (HPLC). The half maximal inhibitory concentration (IC50) of Wuziyanzong pil...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 25, 2018 Category: Drugs & Pharmacology Source Type: research