LC-MS/MS method for simultaneous determination of linezolid, meropenem, piperacillin and teicoplanin in human plasma samples
Publication date: Available online 21 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Davide Ferrari, Marco Ripa, Simone Premaschi, Giuseppe Banfi, Antonella Castagna, Massimo LocatelliAbstractAntibiotic therapy is a crucial aspect of the management of hospitalized patients, however, current standard dosing protocols have been shown to often attain inadequate plasmatic concentrations which may impair the clinical outcome and promote the selection of multidrug-resistant bacteria. The aim of this study is to establish and validate a robust and fast liquid chromatography-tandem mass spectromet...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry
Publication date: Available online 21 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhen Long, Chen Wei, Zhaoqi Zhan, Xiao Ma, Xiuling Li, Yueqi Li, Jinting Yao, Feng Ji, Changkun Li, Taohong HuangAbstractA liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed to determine simultaneously the bioactive proteins including pertussis toxin (PT) subunits, filamentous hemagglutinin (FHA), pertactin (PRN) and fimbriae (FIM) in diphtheria, tetanus and acellular pertussis combined vaccine (DTaP). The trypsin digestion conditions were investigated in detail using PT referen...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a New Method for the Quantitation of Metabolites in the Absence of Chemically Synthetized Authentic Standards
Publication date: Available online 21 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Altares R., F.M. Márquez del Pino, Benedit G., M.J. Guillén, Cuevas C., M.A. Pérez de la Cruz, Aviles P.AbstractA new method for the quantification of metabolites in the absence of a chemically synthetized authentic standard is described herein. Metabolites to be used as reference standards were obtained biologically from microsomes incubation. The method is a stepwise process in which, only the radiolabeled (14C) and non-radiolabeled parent compound are required. Briefly, the separati...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2019 Category: Drugs & Pharmacology Source Type: research

UHPLC-Q-TOF-MS/MS method based on four-step strategy for metabolites of hinokiflavone in vivo and in vitro
In this study, ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) based on four-step strategy was a rapid method for the detection of HF metabolites. A total of 41 metabolites in vivo, 49 metabolites in vitro were characterized. It also verified that intestinal tract exceeds the liver in the biotransformation of HF. More significant, the main metabolic pathways for HF were mainly bio-transformed to various mono-flavone resulting from the rupture of connective C-O bonds, which exhibited a large distinction with other biflavones. Noteworthily, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2019 Category: Drugs & Pharmacology Source Type: research

Using 3D-UPLC-DAD and a new method-verification by adding mixture standard compounds to determine the fingerprint and eight active components of Naoluoxintong decoction
In this study, we developed a comprehensive and reliable analytical method to qualitatively analyze the components in NLXTD. This novel method was based on three-dimensional ultra-fast high performance liquid chromatography coupled with diode array detector (3D-UPLC-DAD) with an additional component validation method via incorporation of the mixture standard compounds during the verification step. In addition, the relationship between active components and “Monarch drug, Minster drug, Assistant drug, Guide drug” were determined. Our results showed that gradient elution with the mobile phase of 0.02% formic acid...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 22, 2019 Category: Drugs & Pharmacology Source Type: research

Development and Validation of an HPLC-UV Method for Simultaneous Determination of Sildenafil and Tramadol in biological fluids: Application to Drug-Drug Interaction Study
In this study, a reversed-phase chromatography was performed at room temperature on a C18 column with a mobile phase composed of 10 mM Na2HPO4 solution (pH 7.5): acetonitrile (45:55, v/v) at a flow rate of 0.8 mL min-1 using caffeine (CAF) as an internal standard. The detector was set at 220 nm. The total analysis time was 6 min. Calibration graphs were linear in the concentration ranges of 0.1–10 and 0.05–10 µg mL-1 with a detection limit of 0.05 and 0.02 µg mL-1 for TMD and SDF, respectively. The method was validated in terms of accuracy, precision, limit ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

Screening techniques for the identification of bioactive compounds in natural products
Publication date: Available online 19 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yanwei Fu, Jiaoyang Luo, Jiaan Qin, Meihua YangAbstractNatural products (NPs) have a long history of clinical use and are rich source of bioactive compounds. The development of tools and techniques for identifying and analyzing NP bioactive compounds to ensure their quality and discover new drugs is thus very important and still in demand. Screening techniques have proven highly useful for screening and analyzing active components in complex mixtures, which rely on cell culture, dialysis, ultrafiltration, ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

Liquid chromatography-tandem mass spectrometry based method development and validation of S016-1271 (LR8P), a novel cationic antimicrobial peptide for its application to pharmacokinetic studies
The objective of the present study was to develop a selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) based bioanalytical method of S016-1271 peptide in mice and human plasma in order to uncover its pharmacokinetic aspects. The chromatographic separation of S016-1271 (FR8P as internal standard) was achieved on a Waters™ X select CSH-C18 column (75 x 3.0 mm, 2.5 µ) using mixture of acetonitrile and triple distilled water (TDW) both containing 0.05 % formic acid as mobile phase. A seven minute linear gradient method was designed to separate analytes from ion suppression at a...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 1: alternative mobile phases and fine tuning of the separation
Publication date: Available online 19 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Evelin Farsang, Amarande Murisier, Krisztián Horváth, Alain Beck, Róbert Kormány, Davy Guillarme, Szabolcs FeketeAbstractCation exchange chromatography (CEX) of therapeutic monoclonal antibodies is generally performed with either salt gradient (MES buffer + NaCl) or using commercial pH gradient buffer. The goal of this study was to find out some alternative buffer systems for CEX separation of mAbs, which may offer alternative selectivity, while maintaining similar...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

A multidirectional investigation of stem bark extracts of four African plants: HPLC-MS/MS profiling and biological potentials
Publication date: Available online 19 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kouadio Bene, Kouadio Ibrahime Sinan, Gokhan Zengin, Alina Diuzheva, József Jekő, Zoltán Cziáky, Muhammad Zakariyyah Aumeeruddy, Jianbo Xiao, Mohamad Fawzi MahomoodallyAbstractThe practice of traditional medicine, especially herbal medicine, is still prevalent across the African continent. Yet, their in-depth pharmacological and chemical exploitation by the scientific community remain a necessity. The aim of the present study was to investigate into the phenolic components, antioxidan...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

Integrating chemical similarity and bioequivalence: a pilot study on quality consistency evaluation of dispensing granule and traditional decoction of Scutellariae Radix by a totality-of-the-evidence approach
This study provides an applicable methodology for quality consistency evaluation of dispensing granule and traditional decoction in TCMs. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

Characterization of in vitro metabolism of focal adhesion kinase inhibitors by LC/MS/MS
Publication date: Available online 19 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Quan Chi, Ling Wang, Dong Xie, Xian WangAbstractFocal adhesion kinase (FAK), a non-receptor tyrosine kinase, is critically involved in cell migration, spreading and proliferation at the early step of various cancers. Small molecule inhibitors of FAK are effective to inhibit its activation in the process of tumor formation in cell. To better understand biotransformation of FAK inhibitors, this work has investigated in vitro phase I metabolism of inhibitors (namely PF-573228, PF-562271 and PF-03814735) by ra...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

Determination and pharmacokinetic study of echinatin by UPLC-MS/MS in rat plasma
In this study, a simple and fast ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the quantification of echinatin in rat plasma was developed, fully validated and subsequently well used in a pharmacokinetic research of echinatin after oral and intravenous administration. Rat plasma samples were operated with a simple one-step acetonitrile precipitation, and licochalcone A was used as the internal standard. Chromatographic separation of echinatin was conducted using an UPLC BEN C18 column and a gradient water (containing 0.1% formic acid)-acetonitrile mobile phase. A Waters XEVO TQS-m...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 20, 2019 Category: Drugs & Pharmacology Source Type: research

New designed special cells for Raman mapping of the disintegration process of pharmaceutical tablets
Publication date: Available online 16 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tereza Čapková-Helešicová, Tomáš Pekárek, Marek Schöngut, Pavel MatějkaAbstractThe goal of this study was the methodological development of Raman microscopy application for advanced and reliable monitoring of pharmaceutical tablets disintegration in aqueous media. First step of the development involved new design and testing of static, flow cell and tablet holders. On-line Raman mapping method enables observation of the tested tablet interphase changes, part...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 17, 2019 Category: Drugs & Pharmacology Source Type: research

Quantification of co-, n-, and ad-lupulone in hop-based dietary supplements and phytopharmaceuticals and modulation of their contents by the extraction method
Publication date: Available online 16 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Carolin Schulz, Chafia Chiheb, Monika PischetsriederAbstractHop β-bitter acids (lupulones) are health-beneficial components of Humulus lupulus L. showing, for example, antidepressant-like effects in vitro. Despite of the widespread use of hops for medicinal purposes, the concentrations of lupulones in hop-based drugs have not been reported yet. The present study developed, validated, and applied a method with external calibration, which allows for the first time separate quantification of co-, n-, and...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 17, 2019 Category: Drugs & Pharmacology Source Type: research

Therapeutic drug monitoring of teicoplanin using an LC–MS/MS method: Analysis of 421 measurements in a naturalistic clinical setting
Publication date: 15 April 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 167Author(s): Jaewan Jung, Kyunghoon Lee, Jongwon Oh, Rihwa Choi, Hye In Woo, Hyung-Doo Park, Cheol-In Kang, Yae-Jean Kim, Soo-Youn LeeAbstractTeicoplanin is a glycopeptide antibiotic used for treatment of severe Gram-positive bacterial infection. The aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC–MS/MS) method for therapeutic drug monitoring (TDM) of teicoplanin and to review our clinical experience.We established an LC–MS/MS method to analyze serum concentration ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 16, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid identification of triptolide in Tripterygium Wilfordii products by gold immunochromatographic assay
Publication date: Available online 14 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yaping Duan, Jiaoyang Luo, Congmin Liu, Linan Shan, Xiaowen Dou, Shihai Yang, Meihua YangAbstractTriptolide colloidal gold immunoassay strip was prepared by using colloidal gold labeled monoclonal antibody purified with saturated ammonium sulfate, triptolide succinate-bovine serum albumin and sheep anti-mouse IgG for the first time in our lab. Under optimized conditions, the detection limit of TP strip prepared in this study were determined to be 1 μg/mL and the total analysis time (including sample pre...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolic profiling of icariin in rat feces, urine, bile and plasma after oral administration using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
This study aimed at identifying the in vivo metabolites and the metabolic profiling in rats after oral administration at a dose of icariin (100 mg/kg) with the aid of an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS/MS) and metabolynxTM software. A total of 25 metabolites were detected and 4 of them were compared with standard substances. Among them, 10 metabolites were reported for the first time. The results indicated that the principal metabolism pathways of icariin in rat were hydroxylation and the conjugation with glucuronide. It also confi...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 15, 2019 Category: Drugs & Pharmacology Source Type: research

In vitro and in vivo pharmacokinetics and metabolism of MK-8353 by liquid chromatography combined with diode array detector and Q-Exactive-Orbitrap tandem mass spectrometry
This study provides overall in vitro and in vivo profiles of MK-8353, which is of great help in understanding its PK/PD profiles and in predicting human pharmacokinetic profiles. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 14, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of 44 pharmaceuticals consumed by elderly using liquid chromatography coupled to tandem mass spectrometry
This study describes the analytical conditions to determine the outmost consumed pharmaceuticals in wastewaters released from senior residences. (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 14, 2019 Category: Drugs & Pharmacology Source Type: research

LC-ESI/LTQOrbitrap/MS/MS and GC-MS profiling of Stachys parviflora L. and evaluation of its biological activities
In this study, chemical composition, antimicrobial, antioxidant, and antiproliferative activities of the methanolic extract and essential oil (EO) of Stachys parviflora L. (S. parviflora) were evaluated. Qualitative analysis of metabolites of S. parviflora methanolic extract was studied using liquid chromatography coupled to high resolution mass spectrometry (LC-ESI/LTQOrbitrap/MS), evidencing the presence of phenolic acids and flavonoids derivatives. The EO was analyzed using gas chromatography coupled to mass spectrometry (GC/MS). Eighty-seven compounds were characterized in the EO of S. parviflora, of which α-terp...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 14, 2019 Category: Drugs & Pharmacology Source Type: research

Quantification of eight benzodiazepines in human breastmilk and plasma by liquid-liquid extraction and liquid-chromatography tandem mass spectrometry: Application to evaluation of alprazolam transfer into breastmilk
Publication date: Available online 13 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ayako Furugen, Ayako Nishimura, Masaki Kobayashi, Takeshi Umazume, Katsuya Narumi, Ken IsekiAbstractBreastfeeding is strongly encouraged for infant and maternal health. Benzodiazepines (BZDs) are widely prescribed drugs for symptoms, such as anxiety and insomnia, which many women could experience during the postpartum period. However, limited information is currently available to evaluate the transfer of different BZDs into breastmilk. In order to assess the proprieties of this medication during breastfeed...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 14, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolomic study reveals the acute hypotensive effect of S-1-propenylcysteine accompanied by alteration of the plasma histidine level in spontaneously hypertensive rats
Publication date: Available online 12 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Toshiaki Matsutomo, Mitsuyasu Ushijima, Kayo Kunimura, Masahiro OhtaniAbstractOur previous study has shown that a single dose of S-1-propenylcysteine (S1PC) exerted an antihypertensive effect in spontaneously hypertensive rats (SHR), while its mode of action remained to be further investigated. The aim of this study was to explore the potential mechanism of the antihypertensive effect of S1PC in SHR using a liquid chromatography-mass spectrometry (LC-MS)-based metabolomic approach. Blood samples were seria...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 13, 2019 Category: Drugs & Pharmacology Source Type: research

Application of an HPLC-MS/MS method for Teicoplanin drug substance and related impurities, part 2: identity assignment of related impurities
Publication date: Available online 13 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sara Tengattini, Federica Corana, Davide Bianchi, Giorgio Marrubini, Raffaella Colombo, Sandra Furlanetto, Marco Terreni, Caterina TemporiniAbstractA liquid chromatographic MS-compatible method was applied to the structural elucidation of Teicoplanin for identification CRS components. The method, previously developed by our group, involves the use of LiChrospher 100 RP-18 column with a mobile phase composed of ammonium formate 25 mM at pH 6.00 and acetonitrile (ACN).All the peaks with a 0.10% UV are...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 13, 2019 Category: Drugs & Pharmacology Source Type: research

Analysis of amino acids in blood by combining zeolitic imidazolate framework-8-based solid phase extraction and capillary electrophoresis
Publication date: Available online 13 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zhenqun Li, Jia Wu, Li JiaAbstractDetection of amino acids (AAs) in blood is helpful to diagnose some diseases. In this work, a method is developed for determination of trace amounts of AAs in bovine blood by combining solid phase extraction (SPE) and capillary electrophoresis (CE). Zeolitic imidazolate framework-8 (ZIF-8) nanoparticles are prepared and used as adsorbents to simultaneously extract three AAs (tryptophan, tyrosine, phenylalanine). Under the optimum extraction conditions, the extraction effic...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 13, 2019 Category: Drugs & Pharmacology Source Type: research

Phytochemical profile of Syzygium formosum (Wall.) Masam leaves using HPLC–PDA–MS/MS and a simple HPLC–ELSD method for quality control
Publication date: Available online 11 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Thi Phuong Duyen Vu, Trong Quan Khong, Thi Minh Nguyet Nguyen, Young Ho Kim, Jong Seong KangAbstractSyzygium formosum (SF) leaves have been used for thousands of years in traditional Vietnamese medicine for the treatment of allergy or skin rash. However, the role of the phytochemical profile of SF leaves on their activities is poorly understood. Additionally, there is currently no quality control method for this herbal material, which is required by the pharmaceutical industry. Therefore, this study aimed ...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 12, 2019 Category: Drugs & Pharmacology Source Type: research

Identification and Characterization of Unknown Degradation Impurities in Beclomethasone Dipropionate Cream Formulation using HPLC, ESI-MS and NMR
Publication date: Available online 10 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Sambandan Elumalai, Kathavarayan Thenmozhi, Sellappan Senthilkumar, Jentaie Shiea, Ponnusamy Vinoth KumarAbstractThe present study focuses on identifying the degradation profile and pathways of unknown impurities from beclomethasone dipropionate (BDP) topical cream formulation reported under accelerated stability conditions. Six degradation impurities were observed during the accelerated stability testing of BDP topical cream formulation, and these thermally labile degradation impurities were primarily ide...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 11, 2019 Category: Drugs & Pharmacology Source Type: research

Application of integrative cloud point extraction and concentration for the analysis of polyphenols and alkaloids in mulberry leaves
Publication date: Available online 10 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Na Guo, Yi-Wei Jiang, Ping Kou, Zhi-Ming Liu, Thomas Efferth, Yan-Yan Li, Yu-Jie FuAbstractA simple and efficient method based on cloud point extraction and concentration combined with high performance liquid chromatography was developed for the simultaneous separation and determination of five target compounds (deoxynojirimycin, chlorogenic acid, rutin, isoquercitrin and astragalin) in mulberry leaves samples. Firstly, to obtain a high extraction rate, the ultrasound assisted extraction was developed on a...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 11, 2019 Category: Drugs & Pharmacology Source Type: research

Metabolic shift of Staphylococcus aureus under sublethal dose of methicillin in the presence of glucose
This study demonstrated that a combination of glucose and a sublethal dose of antibiotic can reduce the survivability of S. aureus in a glucose concentration-dependent manner. Mass spectrometry-based targeted metabolic profiling detected massive metabolic profile shift of both methicillin-susceptible and resistant S. aureus after methicillin and glucose co-treatment. The dramatic alteration of metabolites from these metabolic pathways can be detected when 10 mg/L or higher concentration of glucose were added to methicillin treated culture. Our data also indicated that multiple biochemical metabolic pathways, includi...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 9, 2019 Category: Drugs & Pharmacology Source Type: research

Validation of LC–MS/MS methods for the determination of mirabegron and eight metabolites in human plasma in a paediatric population
Publication date: Available online 7 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): John Meijer, Tom van den Berg, Robert Huls, Vincent Hofstede, Willem Niesing, Cas van den Beld, Walter KrauwinkelAbstractMirabegron is the first registered β3-adrenoceptor agonist for treatment of overactive bladder as an alternative to antimuscarinics. Previously four liquid chromatography-tandem mass spectrometry assays were published to determine mirabegron and eight metabolites (M5, M8, M11-M16) in human plasma. In order to support paediatric development, the assays were further optimized to reduce...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 8, 2019 Category: Drugs & Pharmacology Source Type: research

In vitro metabolic profiles of motolimod by using liquid chromatography tandem mass spectrometry: metabolic stability, metabolite characterization and species comparison
Publication date: Available online 7 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Zeyun Li, Songfeng Zhao, Yongliang Yuan, Lizhen Zhang, Zhizhen Song, Xin Tian, Xiaojian ZhangAbstractMotolimod (VTX-2337) is an agonist of toll-like receptor 8 (TLR8) with potential immune-stimulating and antineoplastic activities. The purpose of this study was to investigate the in vitro metabolic profiles of VTX-2337. The average in vitro T1/2 values were 6.93, 8.71, 7.39, 2.85, and 10.58 min in the liver microsomes of mouse, rat, dog, monkey and human respectively, suggesting that VTX-2337 suffere...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 8, 2019 Category: Drugs & Pharmacology Source Type: research

A comparative study of UHPLC/Orbitrap MS metabolomics profiles and biological properties of Asphodeline taurica from Bulgaria and Turkey
Publication date: Available online 7 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Irina Lazarova, Gokhan Zengin, Reneta Gevrenova, Paraskev Nedialkov, Ina Aneva, Muhammad Zakariyyah Aumeeruddy, Mohamad Fawzi MahomoodallyAbstractThe present investigation attempts to compare the pharmacological properties and phytochemical profile of four extracts (ethyl acetate, dichloromethane, methanol, and water) of Asphodeline taurica (Pall.) Endl. roots from Bulgaria and Turkey. The Bulgarian ethyl acetate extract displayed the highest antioxidant activity in the DPPH, CUPRAC, and phosphomolybdenum a...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 8, 2019 Category: Drugs & Pharmacology Source Type: research

Systematic screening and characterization of prototype constituents and metabolites of triterpenoid saponins of Caulopphyllum Robustum Maxim using UPLC-LTQ Orbitrap MS after oral administration in rats
Publication date: Available online 6 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shaowa Lü, Shiyu Zhao, Min Zhao, Yuyan Guo, Guoyu Li, Bingyou Yang, Qiuhong Wang, Haixue KuangABSTRACTTriterpenoid saponins are the main bioactive components in Caulopphyllum Robustum Maxim (CRM), and they have been reported to have extensive pharmacological properties, such as anti-inflammatory, immunomodulatory, and anti-tumor effects. Cauloside C, Cauloside D, Leonticin D and Cauloside H are the main active chemical constituents of CRM in the treatment of rheumatoid arthritis (RA). However, their me...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of the potent anti-tuberculosis regimen—pyrazinamide, ethambutol, protionamide, clofazimine in beagle dog plasma using LC-MS/MS method coupled with 96-well format plate
Publication date: Available online 6 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shengyuan Wu, Liai Lan, Jingjing Jiang, Xianting Ding, Chih-Ming Ho, Yuefen Lou, Guorong FanAbstractTuberculosis is one of the top concerns in the world and acutely threatens human health. A new potent candidate regimen containing pyrazinamide (PZA), ethambutol (EMB), protionamide (PTO) and clofazimine (CFZ) was proposed by Parabolic Response Surface / Feedback System Control (FSC / PRS) system and showed excellent outcomes in vitro and vivo studies. Here, a convenient liquid chromatography coupled with tan...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2019 Category: Drugs & Pharmacology Source Type: research

Role of esterase mediated hydrolysis of simvastatin in human and rat blood and its impact on pharmacokinetic profiles of simvastatin and its active metabolite in rat
Publication date: Available online 6 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ziwei Li, Jun Zhang, Zhang Yufeng, Zuo ZhongAbstractSimvastatin is known as a pro-drug, which could be hydrolyzed by esterases to its active form, simvastatin acid. Although pharmacokinetics of simvastatin and simvastatin acid have been widely studied, hydrolysis of simvastatin to simvastatin acid during blood sampling and plasma preparation has been overlooked in the previous studies, leading to underestimation of simvastatin concentration and overestimation of simvastatin acid concentration in plasma. Sin...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2019 Category: Drugs & Pharmacology Source Type: research

Hierarchical extraction and simultaneous determination of flavones and triterpenes in different parts of Trichosanthes kirilowii Maxim. by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
Publication date: Available online 6 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Huang-qin Zhang, Pei Liu, Jin-ao Duan, Ling Dong, Er-xin Shang, Da-wei Qian, Ping Xiao, Ming Zhao, Wei-wen LiAbstractTrichosanthin (root of Trichosanthes kirilowii Maxim.) has received much attention, but T. kirilowii has received little systematic analysis. A comprehensive evaluation of flavones and triterpenes in nine different parts of T. kirilowii would, thus, be of value to fully understand the potential medicinal properties of T. kirilowii. A hierarchical extraction—simultaneous determination me...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 7, 2019 Category: Drugs & Pharmacology Source Type: research

development of the lc-ms/ms method for determining the p-cresol level in plasma
Publication date: Available online 31 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Natalia Korytowska, Aleksandra Wyczałkowska-Tomasik, Agnieszka Wiśniewska, Leszek Pączek, Joanna GiebułtowiczAbstractp-Cresol is a protein-bound uremic retention solute that originates in the intestine through bacterial metabolism and accumulates throughout the body in case of kidney failure. To date, there has been no method to analyze unconjugated p-cresol concentration in the blood with a limit of detection lower than 75 pg. Thus, the aim of this study was to develop and validate a novel liqui...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 5, 2019 Category: Drugs & Pharmacology Source Type: research

Phage displayed mimotope peptide-based immunosensor for green and ultrasensitive detection of mycotoxin deoxynivalenol
Publication date: Available online 31 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jiaxiang Yan, Qiang Shi, Kaihao You, Yanping Li, Qinghua HeAbstractImmunoassays play an important role in the monitoring and detection of mycotoxins in food. However, mycotoxin conjugates in the immunoassay system synthesized with toxic mycotoxin as raw material has seriously harmed the environment and human health during and after the performance. In this work, using mycotoxin deoxynivalenol (DON) as a model happen, a selected DON mimotope (phage-displayed peptide) that specifically binds to anti-DON antib...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 5, 2019 Category: Drugs & Pharmacology Source Type: research

A novel reversed-phase high-performance liquid chromatographic assay for the simultaneous determination of imipenem and meropenem in human plasma and its application in TDM
Publication date: Available online 31 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Le Zou, Fanqi Meng, Lin Hu, Qi Huang, Min Liu, Tao YinAbstractA rapid and specific reversed-phase high-performance liquid chromatographic (RP-HPLC) assay with UV detection has been developed and validated for the simultaneous determination of imipenem and meropenem in human plasma. The extraction process was performed through protein precipitation method using acetonitrile and dichloromethane, and the recoveries of quality controls (QCs) were> 91.5%. Isocratic elution followed by gradient elution of acet...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 5, 2019 Category: Drugs & Pharmacology Source Type: research

Therapeutic Drug Monitoring of Teicoplanin Using an LC-MS/MS Method: Analysis of 421 Measurements in a Naturalistic Clinical Setting
Publication date: Available online 4 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jaewan Jung, Kyunghoon Lee, Jongwon Oh, Rihwa Choi, Hye In Woo, Hyung-Doo Park, Cheol-In Kang, Yae-Jean Kim, Soo-Youn LeeAbstractTeicoplanin is a glycopeptide antibiotic used for treatment of severe Gram-positive bacterial infection. The aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for therapeutic drug monitoring (TDM) of teicoplanin and to review our clinical experience.MethodsWe established an LC-MS/MS method to analyze serum concentratio...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 5, 2019 Category: Drugs & Pharmacology Source Type: research

Serum metabolomics model and its metabolic characteristics in patients with different syndromes of dyslipidemia based on nuclear magnetic resonance
This study identified the characteristics of serum metabolic model of patients with different syndromes of dyslipidemia and the potential differential metabolites and characteristic metabolic characteristics of syndromes in order to understand the biological characteristics of patients with dyslipidemia of SKYD and PDR.Graphical abstract (Source: Journal of Pharmaceutical and Biomedical Analysis)
Source: Journal of Pharmaceutical and Biomedical Analysis - February 4, 2019 Category: Drugs & Pharmacology Source Type: research

The power of hyphenated chromatography—Time of flight mass spectrometry for unequivocal identification of spirostanes in bodybuilding dietary supplements
Publication date: Available online 1 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Bharathi Avula, Amar G. Chittiboyina, Ji-Yeong Bae, Saqlain Haider, Yan-Hong Wang, Mei Wang, Jianping Zhao, Patricia A. Deuster, Ikhlas A. KhanAbstractA previously unidentified purported botanical ingredient was found in dietary supplements marketed for anabolic benefits. In an attempt to assess the ‘naturalness’ of a group of steroid-like compounds called laxogenins, a UHPLC-QToF method was developed. Several dietary supplements claim to contain 5α-hydroxy laxogenin, which is a derivative...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 2, 2019 Category: Drugs & Pharmacology Source Type: research

Solution degradant of mirabegron extended release tablets resulting from a Strecker-like reaction between mirabegron, minute amounts of hydrogen cyanide in acetonitrile, and formaldehyde in PEG during sample preparation
Publication date: Available online 1 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Jinsheng Lin, Tianpei Huang, Mengxue Feng, Dan Li, Danna Zhao, Jichao Wang, Ji Jianyang, Wenquan Zhu, Min LiAbstractDuring the related substances testing of mirabegron extended release tablets, an unknown peak was observed in HPLC chromatograms in a level exceeding the identification threshold. By using a strategy that combines LC–PDA/UV-MSn with mechanism-based stress studies, the unknown peak was rapidly identified as cyanomethyl mirabegron, a solution degradant that is caused by a Strecker-like rea...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 2, 2019 Category: Drugs & Pharmacology Source Type: research

Structure Elucidation of Novel Degradation Products of Thiocolchicoside by NMR Spectroscopy
Publication date: Available online 31 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): V.V.S.R.N. Anji Karun Mutha, V.V RavichandraReddy, Prabhakar S. Achanta, Deviprasad Rendedula, Chakravarthy Chandra, Nagul Meera Shaik, Muralidharan Kaliyaperumal, Raghu Babu Korupolu, Susheela Bai Gajbhiye, Chidananda Swamy RumallaAbstractThiocolchicoside is a natural product analogue often used for its spasmolytic action. To know more about its stability under various stress conditions, the drug was stirred in acid, base and peroxide solutions. In acid hydrolysis, two products were obtained and in both, t...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 1, 2019 Category: Drugs & Pharmacology Source Type: research

Ex-vivo intestinal absorption study of boswellic acid, cyclodextrin complexes and poloxamer solid dispersions using everted gut sac technique
Publication date: Available online 1 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Tambe Amruta, Mokashi Priyanka, Pandita NancyAbstractAcetyl- Keto-β-boswellic acid (AKBA) is a pentacyclic triterpenic acid found in gum resin of Boswellia serrata. Even though it is shown to have anti-inflammatory activity, its bioavailability gets limited due to its poor aqueous solubility and permeability. The present study, hence, deals in enhancement of the intestinal absorption of AKBA from total boswellic acid fraction (TA fraction) using cyclodextrin (CD) and poloxamer solid dispersion (PXM SDs...
Source: Journal of Pharmaceutical and Biomedical Analysis - February 1, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of volatile organic compounds in SW620 colorectal cancer cells and tumor-bearing mice
Publication date: Available online 30 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guiyue Wang, Yuhang Li, Miao Liu, Nana Guo, Ci Han, Desheng Liu, Dandan Li, Mengyuan Yang, Yahui Peng, Yansong Liu, Kaijiang Yu, Changsong WangAbstractEarly diagnosis and early treatment are important factors in reducing colorectal cancer (CRC) metastasis and mortality. Volatile organic compounds (VOCs) released by the human body have great potential for use in clinical diagnosis and therapeutic monitoring for CRC. The aim of our study was to identify VOCs with high specificity and high sensitivity for CRC ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2019 Category: Drugs & Pharmacology Source Type: research

Identification of anti-inflammatory components in Sinomenii Caulis based on spectrum-effect relationship and chemometric methods
Publication date: Available online 30 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lan-Jin Wang, Zheng-Meng Jiang, Ping-Ting Xiao, Jian-Bo Sun, Zhi-Ming Bi, E-Hu LiuAbstractThe present study aimed to identify the anti-inflammatory components in Sinomenii Caulis (SC) based on spectrum-effect relationship and chemometric methods. A phytochemical investigation of SC extract was performed firstly and afforded eleven potential bioactive compounds. The HPLC fingerprints of 19 batches of SC samples were evaluated by the chemometric methods such as similarity analysis (SA) and hierarchical cluste...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2019 Category: Drugs & Pharmacology Source Type: research

NADES-MEDIATED FOLK PLANT EXTRACTS AS NOVEL ANTIFUNGAL AGENTS AGAINST Candida albicans
Publication date: Available online 30 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Magdalena Espino, Manuel Solari, María de los Ángeles Fernández, Joana Boiteux, María Roxana Gómez, María Fernanda SilvaAbstractCandida albicans is an opportunistic pathogenic yeast commonly found in mouth, gastrointestinal tract and vagina. Under certain conditions, it causes skin, mucosal and systemic infections. With growing concern over the emergence of resistant strains to conventional antifungals, the development of novel antifungal agents for the management o...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2019 Category: Drugs & Pharmacology Source Type: research

Sniffing Out Resistance – Rapid Identification of Urinary Tract Infection-Causing Bacteria and their Antibiotic Susceptibility Using Volatile Metabolite Profiles
Publication date: Available online 31 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Amy Smart, Ben de Lacy Costello, Paul White, Matthew Avison, Claire Batty, Claire Turner, Raj Persad, Norman RatcliffeAbstractAntibiotic resistance is set to be an unprecedented threat to modern medicine. ‘Sniffing’ bacteria potentially offers a rapid way to determine susceptibility. A successful proof-of-principle study is described, using thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) to ‘smell’ cephalexin and ciprofloxacin resistant and sensitive Urinary Tract ...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2019 Category: Drugs & Pharmacology Source Type: research

A Novel Method using Nuclear Magnetic Resonance for Plasma Protein Binding Assessment in Drug Discovery Programs
Publication date: Available online 31 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mariana Gallo, Sara Matteucci, Nadine Alaimo, Erica Pitti, Maria V. Orsale, Vincenzo Summa, Daniel O. Cicero, Edith MonteagudoAbstractA new methodology based on Nuclear Magnetic Resonance (NMR) was developed to determine plasma protein binding (PPB) of drug candidates in drug discovery programs. A strong correlation was found between the attenuation of NMR signals of diverse drugs in the presence of different plasma concentrations and their fraction bound (fb) reported in the literature. Based on these resu...
Source: Journal of Pharmaceutical and Biomedical Analysis - January 31, 2019 Category: Drugs & Pharmacology Source Type: research