Improvement of Spinosad Production upon Utilization of Oils and Manipulation of β-Oxidation in a High-Producing < b > < i > Saccharopolyspora spinosa < /i > < /b > Strain
Spinosad, a member of polyketide-derived macrolides produced in the actinomyceteSaccharopolyspora spinosa, has been developed as a broad-spectrum and effective insecticide. The β-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (51 1.64 mg/L) and camellia oil (520.07 mg/L) compared to the control group without oil (285.76 mg/L) and soybean oil group (398.11 mg/L). It also revea...
Source: Journal of Molecular Microbiology and Biotechnology - May 4, 2018 Category: Microbiology Source Type: research

Draft Genome Sequence of < b > < i > Bacillus cereus < /i > < /b > CITVM-11.1, a Strain Exhibiting Interesting Antifungal Activities
Bacillus cereus is a gram-positive, spore-forming bacterium possessing an important and historical record as a human-pathogenic bacterium. However, several strains of this species exhibit interesting potential to be used as plant growth-promoting rhizobacteria. Here, we report the draft genome sequence ofB. cereus strain CITVM-11.1, which consists of 37 contig sequences, accounting for 5,746,486 bp (with a GC content of 34.8%) and 5,752 predicted protein-coding sequences. Several of them could potentially be involved in plant-bacterium interactions and may contribute to the strong antagonistic activity shown by this strain...
Source: Journal of Molecular Microbiology and Biotechnology - April 25, 2018 Category: Microbiology Source Type: research

Isolation and Characterization of a Lytic Bacteriophage (vB_PmiS-TH) and Its Application in Combination with Ampicillin against Planktonic and Biofilm Forms of < b > < i > Proteus mirabilis < /i > < /b > Isolated from Urinary Tract Infection
This study demonstrates that using a combination of phage and antibiotic could be significantly more effective against planktonic and biofilm forms ofP. mirabilis (TH).J Mol Microbiol Biotechnol 2018;28:37 –46 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - April 4, 2018 Category: Microbiology Source Type: research

Improved Production of Highly Active and Pure Human Creatine Kinase MB
Human creatine kinase MB (hCKMB) is one of the most preferred biomarkers used for the diagnosis of acute coronary syndrome due to its high sensitivity and specificity. The increasing need for highly purified and biologically active hCKMB in the field of diagnostics makes its production valuable. Currently, the production of hCKMB is mainly achieved in methylotrophic yeast,Pichia pastoris, because the production inEscherichia coli is challenging and generally yields an inactive enzyme with a low quantity. With the aim of finding the best way for the high-yield production of active hCKMB inE. coli, an efficient strategy was ...
Source: Journal of Molecular Microbiology and Biotechnology - March 14, 2018 Category: Microbiology Source Type: research

Cloning and Characterization of a Novel N-acetylglucosaminidase (AtlD) from Enterococcus faecalis
This study will aid in the design of new antibacterial agents of natural origin to combat food-borne diseases, and it could be used as an industrial or hospital hygiene agent as well.J Mol Microbiol Biotechnol 2018;28:14 –27 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - March 6, 2018 Category: Microbiology Source Type: research

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus < b > < i > Phanerochaete chrysosporium < /i > < /b > Overexpressing Laccases and Peroxidases
Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production,Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the con...
Source: Journal of Molecular Microbiology and Biotechnology - February 27, 2018 Category: Microbiology Source Type: research

Contents
J Mol Microbiol Biotechnol 2017;27:I –IV (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - February 22, 2018 Category: Microbiology Source Type: research

High Expression of Human Cathepsin S by Recombinant < b > < i > Pichia pastoris < /i > < /b > with Cod Skin as an Organic Co-Nitrogen Source
Human cathepsin S production by recombinantPichia pastoris using cod skin as the co-nitrogen source was investigated in this study. The addition of carbon sources of glycerol in the fed-batch phase and of methanol in the induction stage was also investigated. A new approach to the highly expression of human cathepsin S was developed using 90 g/L of cod skin (wet weight). After 24 h of the initial fermentation, 4% glycerol (v/v, glycerol/culture) was added once to enhance the cell density (OD600) in the cultivation. Then, adding and maintaining methanol at 0.5% (v/v, methanol/cultivation) after about 48 h of fermentation ac...
Source: Journal of Molecular Microbiology and Biotechnology - February 6, 2018 Category: Microbiology Source Type: research

Identification of Rifampicin Resistance Mutations in < b > < i > Escherichia coli < /i > < /b > , Including an Unusual Deletion Mutation
Rifampicin is an effective antibiotic against mycobacterial and other bacterial infections, but resistance readily emerges in laboratory and clinical settings. We screenedEscherichia coli for rifampicin resistance and identified numerous mutations to the gene encoding the β-chain of RNA polymerase (rpoB), including an unusual 9-nucleotide deletion mutation. Structural modeling of the deletion mutant indicates locations of potential steric clashes with rifampicin. Sequence conservation in the region near the deletion mutation suggests a similar mutation may also confer resistance during the treatment of tuberculosis.J ...
Source: Journal of Molecular Microbiology and Biotechnology - January 16, 2018 Category: Microbiology Source Type: research

Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment.J Mol Microbiol Biotechnol 2017;27:350-3...
Source: Journal of Molecular Microbiology and Biotechnology - December 19, 2017 Category: Microbiology Source Type: research

Comparative Analyses of Transport Proteins Encoded within the Genomes of Bdellovibrio bacteriovorus HD100 and Bdellovibrio exovorus JSS
Bdellovibrio, δ-proteobacteria, includingB. bacteriovorus (Bba) andB. exovorus (Bex), are obligate predators of other Gram-negative bacteria. While Bba grows in the periplasm of the prey cell, Bex grows externally. We have analyzed and compared the transport proteins of these 2 organisms based on the current contents of the Transporter Classification Database (TCDB; www.tcdb.org). Bba has 103 transporters more than Bex, 50% more secondary carriers, and 3 times as many MFS carriers. Bba has far more metabolite transporters than Bex as expected from its larger genome, but there are 2 times more carbohydrate uptake and ...
Source: Journal of Molecular Microbiology and Biotechnology - December 7, 2017 Category: Microbiology Source Type: research

Comparative Analyses of Transport Proteins Encoded within the Genomes of < b > < i > Bdellovibrio bacteriovorus < /i > < /b > HD100 and < b > < i > Bdellovibrio exovorus < /i > < /b > JSS
Bdellovibrio, δ-proteobacteria, includingB. bacteriovorus (Bba) andB. exovorus (Bex), are obligate predators of other Gram-negative bacteria. While Bba grows in the periplasm of the prey cell, Bex grows externally. We have analyzed and compared the transport proteins of these 2 organisms based on the current contents of the Transporter Classification Database (TCDB; www.tcdb.org). Bba has 103 transporters more than Bex, 50% more secondary carriers, and 3 times as many MFS carriers. Bba has far more metabolite transporters than Bex as expected from its larger genome, but there are 2 times more carbohydrate uptake and ...
Source: Journal of Molecular Microbiology and Biotechnology - December 6, 2017 Category: Microbiology Source Type: research

The Dual Carboxymethyl Cellulase and Gelatinase Activities of a Newly Isolated Protein from Brevibacillus agri ST15c10 Confer Reciprocal Regulations in Substrate Utilization
A protein showing endoglucanase-peptidase activity was prepared from a newly isolated bacterium (ST15c10). We identified ST15c10 asBrevibacillus agri based on electron-microscopic images and its 16S-rDNA sequence (GenBank accession No. HM446043), which exhibits 98.9% sequence identity toB. agri(KZ17)/B. formosus(DSM-9885T)/B. brevis. The enzyme was purified to homogeneity and gave a single peak during high-performance liquid chromatography on a Seralose 6B-150 gel-matrix/C-18 column. MALDI-TOF mass-spectrometry and bioinformatics studies revealed significant similarity to M42-aminopeptidases/endoglucanases of the CelM fami...
Source: Journal of Molecular Microbiology and Biotechnology - December 4, 2017 Category: Microbiology Source Type: research

The Dual Carboxymethyl Cellulase and Gelatinase Activities of a Newly Isolated Protein from < b > < i > Brevibacillus agri < /i > < /b > ST15c10 Confer Reciprocal Regulations in Substrate Utilization
A protein showing endoglucanase-peptidase activity was prepared from a newly isolated bacterium (ST15c10). We identified ST15c10 asBrevibacillus agri based on electron-microscopic images and its 16S-rDNA sequence (GenBank accession No. HM446043), which exhibits 98.9% sequence identity toB. agri(KZ17)/B. formosus(DSM-9885T)/B. brevis. The enzyme was purified to homogeneity and gave a single peak during high-performance liquid chromatography on a Seralose 6B-150 gel-matrix/C-18 column. MALDI-TOF mass-spectrometry and bioinformatics studies revealed significant similarity to M42-aminopeptidases/endoglucanases of the CelM fami...
Source: Journal of Molecular Microbiology and Biotechnology - December 1, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic < b > < i > Escherichia coli < /i > < /b > Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - November 30, 2017 Category: Microbiology Source Type: research

< b > < i > ERAP1 < /i > < /b > and < b > < i > PDE8A < /i > < /b > Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - November 30, 2017 Category: Microbiology Source Type: research

Cell Wall-Treated < b > < i > Lactococcus lactis < /i > < /b > Increases the Plasmid Transfer Efficiency of Internal Ribosome Entry Site-Incorporated Lactococcal Bicistronic Vector into DF1 Cells
This study demonstrates that cell wall treatment ofLactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid inL. lactis can be transferred into DF1 cells and that both theVP2 andgfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluores...
Source: Journal of Molecular Microbiology and Biotechnology - November 30, 2017 Category: Microbiology Source Type: research

Archaeal and Bacterial Community Structure in an Anaerobic Digestion Reactor (Lagoon Type) Used for Biogas Production at a Pig Farm
Biogas production from animal waste is an economically viable way to reduce environmental pollution and produce valuable products, i.e., methane and a nutrient-rich organic waste product. An anaerobic digestion reactor for biogas production from pig waste was sampled at the entrance, middle (digestion chamber), and exit of a digester, while the bacterial and archaeal community structure was studied by 16S rRNA gene metagenomics. The number of bacterial operational taxonomic units (OTU)-97% was 3-7 times larger than that of archaeal ones. Bacteria and Archaea found in feces of animals (e.g., Clostridiaceae, Lachnospiraceae,...
Source: Journal of Molecular Microbiology and Biotechnology - November 29, 2017 Category: Microbiology Source Type: research

Novel PCR Assays for the Detection of Biological Agents Responsible for Wheat Rust Diseases: Puccinia triticina and Puccinia striiformis f. sp. tritici
The speciesPuccinia triticina (Pt) andPuccinia striiformis f. sp.tritici(Pst) are devastating cereal pathogens that cause leaf and stripe rust diseases. We developed PCR assays for the species-specific detection of Pt and Pst, 2 biological agents that cause wheat rust disease. For each pathogen, we validated 3 primer sets that target the second largest subunits of the RNA polymerase II (rpb2) and β-tubulin 1 (tub1) genes. The specificities of the primers were verified using naturally infected plant materials with visual symptoms of disease. All primer sets amplified a single DNA fragment of the expected length. The pr...
Source: Journal of Molecular Microbiology and Biotechnology - November 29, 2017 Category: Microbiology Source Type: research

Novel PCR Assays for the Detection of Biological Agents Responsible for Wheat Rust Diseases: < b > < i > Puccinia triticina < /i > < /b > and < b > < i > Puccinia striiformis < /i > < /b > f. sp. < b > < i > tritici < /i > < /b >
The speciesPuccinia triticina (Pt) andPuccinia striiformis f. sp.tritici(Pst) are devastating cereal pathogens that cause leaf and stripe rust diseases. We developed PCR assays for the species-specific detection of Pt and Pst, 2 biological agents that cause wheat rust disease. For each pathogen, we validated 3 primer sets that target the second largest subunits of the RNA polymerase II (rpb2) and β-tubulin 1 (tub1) genes. The specificities of the primers were verified using naturally infected plant materials with visual symptoms of disease. All primer sets amplified a single DNA fragment of the expected length. The pr...
Source: Journal of Molecular Microbiology and Biotechnology - November 28, 2017 Category: Microbiology Source Type: research

Agrowastes as Feedstock for the Production of Endo- β-Xylanase from Cohnella sp. Strain AR92
Members ofCohnellasp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genusCohnella, formed a well-supported cluster withC. thailandensis andC. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitt...
Source: Journal of Molecular Microbiology and Biotechnology - November 23, 2017 Category: Microbiology Source Type: research

Lactic Acid Bacteria from Andean Grain Amaranth: A Source of Vitamins and Functional Value Enzymes
This study demonstrates the potential of LAB to improve the nutritional and functional values of pseudocereal-derived foods.J Mol Microbiol Biotechnol 2017;27:289-298 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - November 22, 2017 Category: Microbiology Source Type: research

Agrowastes as Feedstock for the Production of Endo- β-Xylanase from < b > < i > Cohnella < /i > < /b > sp. Strain AR92
Members ofCohnellasp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genusCohnella, formed a well-supported cluster withC. thailandensis andC. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitt...
Source: Journal of Molecular Microbiology and Biotechnology - November 22, 2017 Category: Microbiology Source Type: research

The Protein Expression Level of a Heterogeneous Gene Inserted in LIPI-1 of the Listeria ivanovii Genome Relies on Its Insertion Orientation
In this study, we inserted theM. tuberculosisantigen geneRv0129cinto LI strains at the same site as the genome but with a different insertion orientation. RT-qPCR and Western blot showed that when the insertion orientation of the heterogeneous gene was opposite to theLIorfXYZgene in the Listeriapathogenicity island 1 in the bacterial genome, the heterogeneous gene could be transcribed well but the protein expression level seemed limited, both in vitro and in vivo. When inserted at an orientation consistent withLIorfXYZ at the same site in the genome, the expected 43-kD protein was observed in vitro as well as in a mouse mo...
Source: Journal of Molecular Microbiology and Biotechnology - November 22, 2017 Category: Microbiology Source Type: research

The Protein Expression Level of a Heterogeneous Gene Inserted in LIPI-1 of the < b > < i > Listeria ivanovii < /i > < /b > Genome Relies on Its Insertion Orientation
In this study, we inserted theM. tuberculosisantigen geneRv0129cinto LI strains at the same site as the genome but with a different insertion orientation. RT-qPCR and Western blot showed that when the insertion orientation of the heterogeneous gene was opposite to theLIorfXYZgene in the Listeriapathogenicity island 1 in the bacterial genome, the heterogeneous gene could be transcribed well but the protein expression level seemed limited, both in vitro and in vivo. When inserted at an orientation consistent withLIorfXYZ at the same site in the genome, the expected 43-kD protein was observed in vitro as well as in a mouse mo...
Source: Journal of Molecular Microbiology and Biotechnology - November 21, 2017 Category: Microbiology Source Type: research

The Membrane Attack Complex/Perforin Superfamily
The membrane attack complex/perforin (MACPF) superfamily consists of a diverse group of proteins involved in bacterial pathogenesis and sporulation as well as eukaryotic immunity, embryonic development, neural migration and fruiting body formation. The present work shows that the evolutionary relationships between the members of the superfamily, previously suggested by comparison of their tertiary structures, can also be supported by analyses of their primary structures. The superfamily includes the MACPF family (TC 1.C.39), the cholesterol-dependent cytolysin (CDC) family (TC 1.C.12.1 and 1.C.12.2) and the pleurotolysin p...
Source: Journal of Molecular Microbiology and Biotechnology - November 16, 2017 Category: Microbiology Source Type: research

Progress in < b > < i > Bacillus subtilis < /i > < /b > Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis
Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, s...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat
This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests forFusarium graminearum,Puccinia spp., andP. tritici-repentis are insufficient.J Mol Microbiol Biotechnol 2017;27:175-189 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Antitumor Effect of a DNA Vaccine Harboring Prostate Cancer-Specific Antigen with IL-12 as an Intramolecular Adjuvant
To improve the lower immune intensity of DNA vaccines, we developed a DNA vaccine based on prostate cancer-specific antigen (PSA), which has been suggested as a potential target for prostate cancer therapy, and enhanced the DNA vaccine potency using interleukin-12 (IL-12) as an intramolecular adjuvant. A series of DNA plasmids encoding human PSA, IL-12, and their conjugates was constructed and injected into female mice intramuscularly, followed by an electric pulse. The humoral and cellular immune responses after immunization were detected by ELISA and ELISPOT, respectively. To evaluate the therapeutic efficacy of these pl...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in < b > < i > Streptomyces < /i > < /b > Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes
This report demonstrates spinosyn production in 2Streptomyces strains and stresses the essential role of rhamnose in this process. This work also provides a potential alternative route for producing spinosyn analogs by means of genetic manipulation in the heterologous hosts.J Mol Microbiol Biotechnol 2017;27:190-198 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Influence of NanoLC Column and Gradient Length as well as MS/MS Frequency and Sample Complexity on Shotgun Protein Identification of Marine Bacteria
Protein identification by shotgun proteomics, i.e., nano-liquid chromatography (nanoLC) peptide separation online coupled to electrospray ionization (ESI) mass spectrometry (MS)/MS, is the most widely used gel-free approach in proteome research. While the mass spectrometer accounts for mass accuracy and MS/MS frequency, the nanoLC setup and gradient time influence the number of peptides available for MS analysis, which ultimately determine the number of proteins identifiable. Here, we report on the influence of (i) analytical column length (15, 25, or 50 cm) coupled to (ii) the applied gradient length (120, 240, 360, 480, ...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Overexpression of a Laccase with Dye Decolorization Activity from Bacillus sp. Induced in Escherichia coli
In this study, a novel laccase gene was cloned from Bacillus sp., analyzed, and functionally expressed in Escherichia coli. The laccase was highly induced in theE. coli expression system with a maximum intracellular activity of 16 U mg-1 protein. The optimal temperature and pH of the purified laccase were 40 °C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonate]) was used as the substrate. The purified laccase showed high stability in the pH range of 3.0-9.0, and retained more than 70% of its activity after 24 h of incubation at 40°C with a pH value of 9.0. Furthermore , the enzyme ...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Rare Human Codons and HCMV Translational Regulation
Restriction of protein synthesis characterizes human cytomegalovirus (HCMV) latency in the human host. In analyzing the molecular factors that hinder HCMV expression, the present study shows that HCMV genes frequently use 6 rare codons, i.e., GCG (Ala), CCG (Pro), CGT (Arg), CGC (Arg), TCG (Ser), and ACG (Thr). In some instances, the rare host codons are clustered along viral nucleotide sequences and represent the majority in sequences encoding short alanine and proline repeats. Given the positive correlation between codon usage, tRNA content, and protein production, the results support the hypothesis that HCMV usage of ra...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic Escherichia coli Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

ERAP1 and PDE8A Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Cell Wall-Treated Lactococcus lactis Increases the Plasmid Transfer Efficiency of Internal Ribosome Entry Site-Incorporated Lactococcal Bicistronic Vector into DF1 Cells
This study demonstrates that cell wall treatment ofLactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid inL. lactis can be transferred into DF1 cells and that both theVP2 andgfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluores...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Cell Wall-Treated < b > < i > Lactococcus lactis < /i > < /b > Increases the Plasmid Transfer Efficiency of Internal Ribosome Entry Site-Incorporated Lactococcal Bicistronic Vector into DF1 Cells
This study demonstrates that cell wall treatment ofLactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid inL. lactis can be transferred into DF1 cells and that both theVP2 andgfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluores...
Source: Journal of Molecular Microbiology and Biotechnology - October 20, 2017 Category: Microbiology Source Type: research

ERAP1 and PDE8A Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 14, 2017 Category: Microbiology Source Type: research

< b > < i > ERAP1 < /i > < /b > and < b > < i > PDE8A < /i > < /b > Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 13, 2017 Category: Microbiology Source Type: research

Expression and Characterization of Serotype 2 < b > < i > Streptococcus suis < /i > < /b > Arginine Deiminase
Conclusions: This study profoundly demonstrated the involvement of ArcA enzymatic activity inS. suis survival under acidic conditions.J Mol Microbiol Biotechnol 2017;27:133-146 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 12, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic Escherichia coli Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - September 11, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic < b > < i > Escherichia coli < /i > < /b > Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - September 8, 2017 Category: Microbiology Source Type: research

Overexpression of a Laccase with Dye Decolorization Activity from Bacillus sp. Induced in Escherichia coli
In this study, a novel laccase gene was cloned from Bacillus sp., analyzed, and functionally expressed in Escherichia coli. The laccase was highly induced in theE. coli expression system with a maximum intracellular activity of 16 U mg-1 protein. The optimal temperature and pH of the purified laccase were 40 °C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonate]) was used as the substrate. The purified laccase showed high stability in the pH range of 3.0-9.0, and retained more than 70% of its activity after 24 h of incubation at 40°C with a pH value of 9.0. Furthermore , the enzyme ...
Source: Journal of Molecular Microbiology and Biotechnology - September 1, 2017 Category: Microbiology Source Type: research

Overexpression of a Laccase with Dye Decolorization Activity from < b > < i > Bacillus < /i > < /b > sp. Induced in < b > < i > Escherichia coli < /i > < /b >
In this study, a novel laccase gene was cloned from Bacillus sp., analyzed, and functionally expressed in Escherichia coli. The laccase was highly induced in theE. coli expression system with a maximum intracellular activity of 16 U mg-1 protein. The optimal temperature and pH of the purified laccase were 40 °C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonate]) was used as the substrate. The purified laccase showed high stability in the pH range of 3.0-9.0, and retained more than 70% of its activity after 24 h of incubation at 40°C with a pH value of 9.0. Furthermore , the enzyme ...
Source: Journal of Molecular Microbiology and Biotechnology - August 31, 2017 Category: Microbiology Source Type: research

Rare Human Codons and HCMV Translational Regulation
Restriction of protein synthesis characterizes human cytomegalovirus (HCMV) latency in the human host. In analyzing the molecular factors that hinder HCMV expression, the present study shows that HCMV genes frequently use 6 rare codons, i.e., GCG (Ala), CCG (Pro), CGT (Arg), CGC (Arg), TCG (Ser), and ACG (Thr). In some instances, the rare host codons are clustered along viral nucleotide sequences and represent the majority in sequences encoding short alanine and proline repeats. Given the positive correlation between codon usage, tRNA content, and protein production, the results support the hypothesis that HCMV usage of ra...
Source: Journal of Molecular Microbiology and Biotechnology - August 31, 2017 Category: Microbiology Source Type: research

Influence of NanoLC Column and Gradient Length as well as MS/MS Frequency and Sample Complexity on Shotgun Protein Identification of Marine Bacteria
Protein identification by shotgun proteomics, i.e., nano-liquid chromatography (nanoLC) peptide separation online coupled to electrospray ionization (ESI) mass spectrometry (MS)/MS, is the most widely used gel-free approach in proteome research. While the mass spectrometer accounts for mass accuracy and MS/MS frequency, the nanoLC setup and gradient time influence the number of peptides available for MS analysis, which ultimately determine the number of proteins identifiable. Here, we report on the influence of (i) analytical column length (15, 25, or 50 cm) coupled to (ii) the applied gradient length (120, 240, 360, 480, ...
Source: Journal of Molecular Microbiology and Biotechnology - August 29, 2017 Category: Microbiology Source Type: research

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in Streptomyces Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes
This report demonstrates spinosyn production in 2Streptomyces strains and stresses the essential role of rhamnose in this process. This work also provides a potential alternative route for producing spinosyn analogs by means of genetic manipulation in the heterologous hosts.J Mol Microbiol Biotechnol 2017;27:190-198 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 21, 2017 Category: Microbiology Source Type: research

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in < b > < i > Streptomyces < /i > < /b > Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes
This report demonstrates spinosyn production in 2Streptomyces strains and stresses the essential role of rhamnose in this process. This work also provides a potential alternative route for producing spinosyn analogs by means of genetic manipulation in the heterologous hosts.J Mol Microbiol Biotechnol 2017;27:190-198 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 18, 2017 Category: Microbiology Source Type: research

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat
This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests forFusarium graminearum,Puccinia spp., andP. tritici-repentis are insufficient.J Mol Microbiol Biotechnol 2017;27:175-189 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 3, 2017 Category: Microbiology Source Type: research