EW ‐7197 prevents ulcerative colitis‐associated fibrosis and inflammation
Here, we investigate the potential therapeutic effects of EW ‐7197 in a murine model of ulcerative colitis. Our results suggest that EW‐7197 has potentially useful therapeutic properties against colitis, with clinically translational potential of inhibiting key pathological responses of inflammation and fibrosis in patients with colitis. AbstractEW ‐7197 is a transforming growth factor‐β type I receptor kinase inhibitor with potential anti‐inflammatory and antifibrotic properties. Here, we investigate the potential therapeutic effects of EW‐7197 in a murine model of ulcerative colitis. EW‐7197 attenuated the...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Maryam M. Binabaj,
Fereshteh Asgharzadeh,
Amir Avan,
Farzad Rahmani,
Atena Soleimani,
Mohammad R. Parizadeh,
Gordon A. Ferns,
Mikhail Ryzhikov,
Majid Khazaei,
Seyed M. Hassanian Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Poly (3 ‐hydroxybutyrate‐co‐3‐hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix
In this study, electrospun PHBV nanofibers were fabricated and characterized and then osteogenic differentiation of the human iPSCs was investigated while cultured on PHBV scaffold.‐MTT, alkaline phosphatase activity, calcium content, gene expression, and western blot evaluations were confirmed osteogenic differentiation of the human iPSCs increased significantly when grown on PHBV nanofibers. AbstractCocell polymers can be the best implants for replacing bone defects in patients. The pluripotent stem cells produced from the patient and the nanofibrous polymeric scaffold that can be completely degraded in the body and it...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Fatemeh Sadat Hosseini,
Fatemeh Soleimanifar,
Amir Aidun,
Seyedeh Elnaz Enderami,
Ehsan Saburi,
Hadi Zare Marzouni,
Mohammad ‐Mehdi Khani,
Arash Khojasteh,
Abdolreza Ardeshirylajimi Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Antibody –drug conjugates (ADCs) for cancer therapy: Strategies, challenges, and successes
Third ‐generation antibody–drug conjugates are more effective, showing lower levels of unconjugated monoclonal antibodies, more‐stable linkers, improved pharmacokinetic properties, and safety profiles. AbstractTargeted delivery of therapeutic molecules into cancer cells is considered as a promising strategy to tackle cancer. Antibody –drug conjugates (ADCs), in which a monoclonal antibody (mAb) is conjugated to biologically active drugs through chemical linkers, have emerged as a promising class of anticancer treatment agents, being one of the fastest growing fields in cancer therapy. The failure of early ADCs led ...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Meghdad Abdollahpour ‐Alitappeh,
Majid Lotfinia,
Tohid Gharibi,
Jalal Mardaneh,
Behrouz Farhadihosseinabadi,
Pegah Larki,
Babak Faghfourian,
Koushan Sineh Sepehr,
Kazem Abbaszadeh‐Goudarzi,
Ghasem Abbaszadeh‐Goudarzi,
Behrooz Johari,
Moha Tags: REVIEW ARTICLE Source Type: research
CtIP promotes G2/M arrest in etoposide ‐treated HCT116 cells in a p53‐independent manner
In this study, we show that knockdown of CtIP, a corepressor of CtBP, promotes cell proliferation and alleviates G2/M phase arrest in etoposide (Eto) ‐treated HCT116 cells. Although the expression of p21 and growth arrest and DNA damage inducible α (GADD45a), which are important targets of p53, was downregulated in CtIP‐deficient HCT116 cells, p53 deletion did not affect G2/M arrest after Eto treatment. In addition, the phosphorylation level s of Ser317 and Ser345 in Chk1 and of Ser216 in CDC25C were lower in CtIP‐deficient HCT116 cells than in control cells after Eto treatment. Our results indicate that CtIP may en...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hongyu Chen,
Jin Shan,
Dandan Chen,
Ruoxi Wang,
Wenjing Qi,
Hailong Wang,
Yueshuang Ke,
Wenguang Liu,
Xianlu Zeng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Long noncoding RNA LINC00460 promotes carcinogenesis via sponging miR ‐613 in papillary thyroid carcinoma
This study was aimed to ascertain the biological role and molecular mechanism of LINC00460 in PTC progression. Our findings revealed that the level of LINC00460 was significantly upregulated in PTC tissues and cell lines, which was positively correlated with advanced tumor –node–metastasis (TNM) stage and lymph node metastasis. Cellular experiments exhibited that knockdown of LINC00460 decreased proliferative, migratory, and invasive abilities of PTC cells. Mechanism assays noted that knockdown of LINC00460 suppressed cell proliferation, migration, and invasion, a nd inhibited expression of sphingosine kinase 2 (SphK2,...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Li Feng,
Bin Yang,
Xiao ‐Di Tang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Intersectin ‐Cdc42 interaction is required for orderly meiosis in porcine oocytes
Our findings support a model where intersectins, through the interaction with Cdc42, modulates the assembly of meiotic apparatus and actin polymerization, consequently ensuring the orderly meiotic progression during porcine oocyte maturation. AbstractIntersectins (ITSNs) have been shown to act as adaptor proteins that govern multiple cellular events via regulating Cdc42 activity. However, it remains to be determined whether the ITSN ‐Cdc42 pathway is functional in porcine oocytes. To address this question, we used a small molecule, ZCL278, to selectively disrupt the ITSN2‐Cdc42 interaction. In the present study, we fi...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xiaoyan Li,
Min Gao,
Yongfu He,
Bo Xiong,
Honglin Liu,
Ling Gu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
PCNA and JNK1 ‐Stat3 pathways respectively promotes and inhibits diabetes‐associated centrosome amplification by targeting at the ROCK1/14‐3‐3σ complex in human colon cancer HCT116 cells
In conclusion, our results suggest that PCNA and JNK1‐Stat3 pathways respectively promotes and feedback inhibits the centrosome amplificati on by targeting at the ROCK1/14‐3‐3σ complex, and NPM serves as an independent signal for the centrosome amplification. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yu Cheng Lu,
Pu Wang,
Jie Wang,
Ronald Ma,
Shao Chin Lee Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
CircRNA ZNF609 functions as a competitive endogenous RNA to regulate FOXP4 expression by sponging miR ‐138‐5p in renal carcinoma
In this study, we investigated whether circ‐ZNF609 may affect cell invasion and proliferation in renal carci noma. Quantitative reverse transcription polymerase chain reaction was performed to detect the expression of circ‐ZNF609 in renal carcinoma cell lines and renal epithelial cells. The direct interaction between microRNA‐138‐5p (miR‐138‐5p) and forkhead box P4 (FOXP4) or circ‐ZNF609 was co nfirmed by luciferase reporter assay and RNA immunoprecipitation assay. We use Cell Counting Kit‐8, 5‐ethynyl‐2′‐deoxyuridine, and Matrigel assays to assess the effect of miR‐138‐5p or circ‐ZNF609 on ce...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yunhe Xiong,
Jiabin Zhang,
Chao Song Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Invasiveness ‐triggered state transition in malignant melanoma cells
AbstractCancer cells are considered to have high morphological heterogeneity in human melanoma tissue. Here, we report that epithelial cancer cells are dominant in different development stages of human melanoma tissues. The cellular and molecular mechanisms that maintain melanoma cells in the epithelial state are further investigated in the A2058 cell line. We find that micropore (8 µm) transwell invasion, but not superficial migration in the scratch assay, can induce remarkable morphological changes between epithelial and mesenchymal melanoma cells within 4 days. The morphological switch is associated with dynamic cha...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Huan Wang,
Yan ‐Guo Zhang,
Jing Ma,
Jun‐Chang Li,
Jian Zhang,
Yao‐Qing Yu Tags: RAPID COMMUNICATION Source Type: research
Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT ‐1‐TREM‐1 pathway in diabetic nephropathy
ConclusionVD can inhibit macrophage transition to the M1 phenotype through the STAT ‐1/TREM‐1 pathway. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xiaoliang Zhang,
Yu Zhao,
Xiaodong Zhu,
Yinfeng Guo,
Ying Yang,
Yuteng Jiang,
Bicheng Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Molecular interaction of NF κB and NICD in monocyte–macrophage differentiation is a target for intervention in atherosclerosis
Inhibition of NF κB regulated notch intracellular domain (NICD), which, in turn, downturned macrophage differentiation. Inhibition of both NFκB–NICD is a potential target for intervention in early stage of atherosclerosis (monocyte to macrophage differentiation), to prevent the furtherance of disease. AbstractThe activation of two transcription factors, NF κB and NICD (notch intracellular domain), plays a crucial role in different stages of atherosclerotic disease progression, from early endothelial activation by modified lipids like oxidized low‐density lipoprotein (oxyLDL) to the imminent rupture of the atheroscle...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Ambika Binesh,
Sivasithamparam Niranjali Devaraj,
Devaraj Halagowder Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
G protein ‐coupled receptor 119 is involved in RANKL‐induced osteoclast differentiation and fusion
In this study, we i nvestigated the functional role of GPR119 in receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclast formation. We found that the GPR119 expression was markedly increased in preosteoclasts and then downregulated in mature osteoclasts. Activation of GPR119 with AS1269574, a pot ent selective agonist for GPR119, inhibited the generation of multinuclear osteoclasts from bone marrow‐derived macrophages. Confirming this observation, targeted silencing ofGPR119 using short hairpin RNA abrogated the AS1269574 ‐mediated suppressive effect on osteoclast formation. GPR119 activation att...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hyun ‐Ju Kim,
Hye‐Jin Yoon,
Ji‐Wan Park,
Xiangguo Che,
Xian Jin,
Je‐Yong Choi Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
MicroRNA: A new player in response to therapy for colorectal cancer
AbstractColorectal cancer (CRC) is one of the important malignancies that result in cancer ‐related deaths worldwide. Multiple lines of evidence have indicated that different responses to therapy in CRC cells led to the failure of the current therapies. Hence, identification of the underlying cellular and molecular pathways involved in the emergence of different responses from CRC cells could contribute to finding and designing new therapeutic platforms to overcome the present limitations. Among the various targets involved in CRC pathogenesis, microRNAs (miRNAs) have key roles in many signaling pathways that are associa...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Mostafa Sarvizadeh,
Ziba V. Malekshahi,
Ebrahim Razi,
Hossein Sharifi,
Nousin Moussavi,
Mohsen Taghizadeh Tags: REVIEW ARTICLE Source Type: research
Isolation and culture of vascular smooth muscle cells from rat placenta
In this study, we described a simple and efficient method for isolating placental vascular smooth muscle cells (PVSMCs) from rat. The method used the magnetic force between a magnet and ferrous ferric oxide (Fe304) to make the separation and extraction of VSMC. The cells isolated using this protocol were identified as smooth muscle cells (SMCs) by immunoreactivity to the SMC markers and by the “hill and valley” morphology. AbstractWe developed a new separation method for isolating placental vascular smooth muscle cells (PVSMCs) from a rat in this study. Our method used the magnetic force between a magnet and ferrous fe...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Chunyu He,
Meili Wang,
Zi Yan,
Suli Zhang,
Huirong Liu Tags: FROM THE BENCH Source Type: research
Downregulation of microRNA ‐592 protects mice from hypoplastic heart and congenital heart disease by inhibition of the Notch signaling pathway through upregulating KCTD10
This study demonstrates that downregulation of miR ‐592 prevents CHD and hypoplastic heart by inhibition of the Notch signaling pathway via negatively binding to KCTD10. AbstractEvidence has demonstrated that the microRNA (miR) may play a significant role in the development of congenital heart disease (CHD). Here, we explore the mechanism of microRNA ‐592 (miR‐592) in heart development and CHD with the involvement of KCTD10 and Notch signaling pathway in a CHD mouse model. Cardiac tissues were extracted from CHD and normal mice. Immunohistochemistry staining was performed to detect positive expression rate of KCTD10....
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xue ‐Feng Pang,
Xue Lin,
Jian‐Jun Du,
Ding‐Yin Zeng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
