Journal of Bioscience and Bioengineering This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Enhancement of metabolic flux toward ε-poly-l-lysine biosynthesis by targeted inactivation of concomitant polyene macrolide biosynthesis in Streptomyces albulus
Publication date: Available online 8 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Kazuya Yamanaka, Yoshimitsu Hamano, Tadao Oikawaε-Poly-l-lysine (ε-PL) produced as a secondary metabolite of Streptomyces albulus has long been used as a natural food preservative in a number of countries, including Japan, the United States, South Korea, and China. To date, numerous studies employing classical biotechnological approaches have been carried out to improve its productivity. Here we report a modern and rational genetic approach to enhancing metabolic flux toward ε-PL biosynthesis. Based on in silico g...
Source: Journal of Bioscience and Bioengineering - January 8, 2020 Category: Biomedical Science Source Type: research

Secretory production of N-glycan-deleted glycoprotein in Aspergillus oryzae
Publication date: Available online 7 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Qiushi Li, Yujiro Higuchi, Kana Tanabe, Yoshinori Katakura, Kaoru TakegawaThe pharmaceutical industry has a high demand for glycoprotein production. The glycoform of glycoproteins is crucial for pharmacological activity. However, in general, cells produce glycoproteins with a heterologous glycoform, which is unfavorable for making uniform, efficacious therapeutic proteins. Here, to produce more glycoproteins with N-glycan uniformity, we applied the GlycoDelete strategy, in which endo-β-N-acetylglucosaminidase (ENGas...
Source: Journal of Bioscience and Bioengineering - January 7, 2020 Category: Biomedical Science Source Type: research

Metabolomics approach for determining potential metabolites correlated with sensory attributes of Melaleuca cajuputi essential oil, a promising flavor ingredient
This study was conducted to identify potential metabolites that are correlated to sensory attributes of MCEO by using the metabolomics approach. The metabolite profiles of thirteen MCEOs from different origins were analyzed by gas chromatography-mass spectrometry while sensory analyses on Cajuputs Candy were conducted by difference-from-control and rate-all-that-apply tests. Sixty metabolites from the MCEO were annotated that includes 1,8-cineole, α-terpineol, caryophyllene, α-pinene, and γ-terpinene. Sensory analysis revealed cooling aftertaste and sweet taste as favorable attributes. Further analysis using Orthogonal ...
Source: Journal of Bioscience and Bioengineering - January 2, 2020 Category: Biomedical Science Source Type: research

Fabrication of contractile skeletal muscle tissues using directly converted myoblasts from human fibroblasts
Publication date: Available online 16 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Kazunori Shimizu, Saki Ohsumi, Tsunao Kishida, Osam Mazda, Hiroyuki HondaTransplantation of stem cell-derived myoblasts is a promising approach for the treatment of skeletal muscle function loss. Myoblasts directly converted from somatic cells that bypass any stem cell intermediary stages can avoid the problem of tumor formation after transplantation. Previously, we reported that co-transduction with the myogenic differentiation 1 (MYOD1) gene and the v-myc avian myelocytomatosis viral oncogene lung carcinoma deriv...
Source: Journal of Bioscience and Bioengineering - December 17, 2019 Category: Biomedical Science Source Type: research

Enzymatic hydrolysis of cellulose recovered from ionic liquid-salt aqueous two-phase system
Publication date: Available online 16 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Kazuhiko Tanimura, Keishi Suga, Yukihiro Okamoto, Hiroshi UmakoshiRegenerated cellulose can be prepared by treatment with an ionic liquid (IL) and an anti-solvent such as water, which significantly enhances the enzymatic hydrolysis in comparison to crystalline cellulose. The IL-aqueous two-phase system (IL-ATPS) is consisted of IL-condensed top phase and salt-condensed bottom phase, which could be suitable to produce regenerated cellulose with smaller amount of IL. Using IL-ATPS with different pH, the enzymatic sac...
Source: Journal of Bioscience and Bioengineering - December 17, 2019 Category: Biomedical Science Source Type: research

Disulfide linked hetero dimeric peptide arrays for screening functional peptides inside cells
Publication date: Available online 13 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Ikko Kozaki, Kazunori Shimizu, Hiroyuki HondaIntracellular functional peptides with important roles in cells have been widely studied as regulators of cellular activity. Previously, we proposed a screening system for identifying intracellular functional peptides, which can be utilized to synthesize various cell-penetrating peptide (CPP)-functional peptide complexes, and the intracellular functions of the peptides can then be determined using various cell based assays. We demonstrated that the activity of a cell dea...
Source: Journal of Bioscience and Bioengineering - December 13, 2019 Category: Biomedical Science Source Type: research

Trichothioneic acid, a new antioxidant compound produced by the fungal strain Trichoderma virens FKI-7573
Publication date: Available online 11 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Rei Miyano, Hirotaka Matsuo, Takayuki Mokudai, Yoshihiko Noguchi, Mayuka Higo, Kenichi Nonaka, Yoshimi Niwano, Toshiaki Sunazuka, Kazuro Shiomi, Yōko Takahashi, Satoshi Ōmura, Takuji NakashimaA new nitrogen-containing compound, trichothioneic acid, was discovered from the metabolites of fungal strain FKI-7573 using a mass spectrometry screening method guided by odd number of molecular weights, which indicates compounds that contain an odd number of nitrogen atoms. Strain FKI-7573 was isolated from soil collected ...
Source: Journal of Bioscience and Bioengineering - December 12, 2019 Category: Biomedical Science Source Type: research

Suppression of lactate production in fed-batch culture of some lactic acid bacteria with sucrose as the carbon source
We report a method for suppression of lactate production by lactic acid bacteria (LAB) in culture. LAB produce lactate to regenerate NAD+ that is consumed during glycolysis. Glucose suppresses NAD+ regeneration pathways other than lactate dehydrogenase and non-glycolytic ATP production pathways. Therefore, the carbon source was changed to sucrose, and fed-batch culture was performed to limit the glycolytic flux and thus suppress lactate production. As a result, lactate productivity (i.e., the amount of lactate produced per amount of grown cell) in the sucrose/fed-batch culture was decreased compared to that in glucose/batc...
Source: Journal of Bioscience and Bioengineering - December 11, 2019 Category: Biomedical Science Source Type: research

Continuous cultivation strategy for yeast industrial wastewater-based polyhydroxyalkanoate production
Publication date: Available online 10 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Aniruddha Bhalerao, Rintu Banerjee, Regina NogueiraPolyhydroxyalkanoates (PHAs) present an eco-friendly alternative for conventional plastics. Industrial wastewater from the food industry is a copious source of organic carbon that can be recovered in the form of PHA. However, the wastewater composition varies considerably among the different industries demanding for an industry-specific investigation of the PHA production process. Wastewater from the yeast industry, besides its high concentration in organic carbon,...
Source: Journal of Bioscience and Bioengineering - December 11, 2019 Category: Biomedical Science Source Type: research

Characterisation of the bacterial community structures of sunki, a traditional unsalted pickle of fermented turnip leaves
Publication date: Available online 9 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Satoru Tomita, Jun Watanabe, Toshihide Nakamura, Akihito Endo, Sanae OkadaThe bacterial community structure in 29 naturally fermented samples of sunki, an unsalted lactic-fermented pickle in Japan, was determined by 16S rRNA gene-targeted metagenomic analysis. The data revealed that genus Lactobacillus was dominant in all samples and various bacterial species, related to Lactobacillus delbrueckii, Lactobacillus plantarum, Lactobacillus buchneri, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus crispatus...
Source: Journal of Bioscience and Bioengineering - December 9, 2019 Category: Biomedical Science Source Type: research

Kinetics on aggregate behaviors of human induced pluripotent stem cells in static suspension and rotating flow cultures
In this study, we elucidated kinetics on aggregate behaviors of two types of hiPSCs (253G1 and 201B7 lines) to clarify the influence of aggregate behaviors on cell growth by comparing aggregate morphology, size of cell aggregates, and kinetic parameters in 72 h culture under static and floating conditions, which were realized by multi-dimple plate and rotating wall vessel, respectively. In the case of 253G1 line under floating condition, aggregate number decreased and size increased drastically during culture time, t = 0–24 h due to coalescence between cell aggregates. The apparent specific growth rate decreased after...
Source: Journal of Bioscience and Bioengineering - December 9, 2019 Category: Biomedical Science Source Type: research

Antibacterial and antibiofilm activity of ursolic acid against carbapenem-resistant Enterobacter cloacae
In this study, we aimed to investigate the mechanisms underlying-antimicrobial activity of UA and its mode of action against CREC. Minimum inhibitory concentrations (MIC) of UA against CREC were determined by the agar dilution method. The antibacterial effect of UA against CREC was elucidated by evaluating changes in intracellular ATP concentration, intracellular pH, membrane potential, membrane integrity, and cell morphology. A crystal violet assay, field emission scanning electron microscopy (FESEM), and confocal laser scanning microscopy (CLSM) were applied to examine the effect of UA on biofilm formation. Furthermore, ...
Source: Journal of Bioscience and Bioengineering - December 7, 2019 Category: Biomedical Science Source Type: research

Sirtuin SirD is involved in α-amylase activity and citric acid production in Aspergillus luchuensis mut. kawachii during a solid-state fermentation process
Publication date: Available online 5 December 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Aoi Miyamoto, Chihiro Kadooka, Kazuki Mori, Yuki Tagawa, Kayu Okutsu, Yumiko Yoshizaki, Kazunori Takamine, Masatoshi Goto, Hisanori Tamaki, Taiki FutagamiSirtuins are understood to play a significant role in growth phase-dependent gene expression. In the study presented here, we examined the sirtuins in the white koji fungus, Aspergillus luchuensis mut. kawachii (Aspergillus kawachii), to examine their role in the regulation of amylolytic enzymes and citric acid production during solid-state culture (koji). Characte...
Source: Journal of Bioscience and Bioengineering - December 7, 2019 Category: Biomedical Science Source Type: research

Nanofabricated versatile electrochemical sensor for Leptospira interrogans detection
In this report, a LipL32 gene based nanofabricated electrochemical sensor for the detection of Leptospira interrogans has been developed using carboxylated multiwalled carbon nanotubes with gold nanoparticles (c-MWCNTs/nanoAu) electrode and graphene quantum dots (GQDs). The c-MWCNTs/nanoAu electrode was linked to GQDs using 4-aminothiophenol (ATP). The surface modifications on the electrode surface were delineated using Raman spectroscopy and field emission scanning electron microscopy (FE-SEM). 5′-Amino (NH2) labeled single stranded DNA (ssDNA) probe was immobilized on the surface of c-MWCNTs/nanoAu/ATP/GQD composite el...
Source: Journal of Bioscience and Bioengineering - November 29, 2019 Category: Biomedical Science Source Type: research

Overexpression of the bioactive lunasin peptide in soybean and evaluation of its anti-inflammatory and anti-cancer activities in vitro
In conclusion, it is feasible and efficient to produce lunasin through a transgenic soybean expression system. Lunasin overexpressing soybean could be consumed as a functional food in the diets of patients with cancer and obesity in the future. (Source: Journal of Bioscience and Bioengineering)
Source: Journal of Bioscience and Bioengineering - November 27, 2019 Category: Biomedical Science Source Type: research