Journal of Bioscience and Bioengineering This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Characterization of a novel type I l-asparaginase from Acinetobacter soli and its ability to inhibit acrylamide formation in potato chips
Publication date: Available online 20 February 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Linshu Jiao, Huibing Chi, Zhaoxin Lu, Chong Zhang, Shir Reen Chia, Pau Loke Show, Yang Tao, Fengxia Lul-Asparaginases have the potential to inhibit the formation of acrylamide, a harmful toxin formed during high temperature processing of food. A novel bacterium which produces l-asparaginase was screened. Type I l-asparaginase gene from Acinetobacter soli was cloned and expressed in Escherichia coli. The recombinant l-asparaginase had an activity of 42.0 IU mL−1 and showed no activity toward l-glutamine and d-aspa...
Source: Journal of Bioscience and Bioengineering - February 20, 2020 Category: Biomedical Science Source Type: research

Development of sake yeast haploid set with diverse brewing properties using sake yeast strain Hiroshima no. 6 exhibiting sexual reproduction
Publication date: Available online 19 February 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Risa Yamasaki, Tetsuya Goshima, Kenji Oba, Muneyoshi Kanai, Ritsushi Ohdoi, Dai Hirata, Takeshi AkaoAmong sake yeast strains, Kyokai no. 7 (K7) and its closely related strains (K7 group) are predominantly used because of their excellent brewing properties. In the sake industrial sector, the need for various types of yeast strains is high. Although crossbreeding is an effective method for generating genetic diversity that should result in diverse characteristics, most K7 group strains lack normal sporulation ability...
Source: Journal of Bioscience and Bioengineering - February 20, 2020 Category: Biomedical Science Source Type: research

Bioethanol production estimated from volatile compositions in hydrolysates of lignocellulosic biomass by deep learning
Publication date: Available online 17 February 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Masaaki KonishiThe cell growth and ethanol production from hydrolysates of various types were estimated from the volatile composition of lignocellulosic biomass by deep neural network (DNN) and the significant compositions estimated by asymmetric autoencoder-decoder (AAE). A six-layer DNN achieved good accuracy with learning and validation losses—0.033 and 0.507, respectively—and estimated overall time courses of yeast growth and ethanol fermentation. The AAE decoded the volatile compositions and represented th...
Source: Journal of Bioscience and Bioengineering - February 18, 2020 Category: Biomedical Science Source Type: research

Vacuum lyophilization preservation and rejuvenation performance of anammox bacteria
Publication date: Available online 7 February 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Hanmin Zhang, Lu Cai, Fan Zhang, Chengcheng Ge, Fenglin YangThe storage of anaerobic ammonia oxidizing bacteria (anammox) plays an important role in the application of anammox. Glycerol, sodium alginate and DMSO were used as the cryoprotectant, and vacuum lyophilization was used to prepare the anammox bacteria powder. Simultaneously, the control experiment was set up with the same protectant and preservation time. Bacteria powders were preserved using vacuum lyophilization and preserved at 4 °C for 60 days. During...
Source: Journal of Bioscience and Bioengineering - February 8, 2020 Category: Biomedical Science Source Type: research

Biosensing capabilities of bioelectrochemical systems towards sustainable water streams: Technological implications and future prospects
Publication date: Available online 8 February 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Surajbhan Sevda, Vijay Kumar Garlapati, Sunandan Naha, Mohita Sharma, Sreemoyee Ghosh Ray, Trichur Ramaswamy Sreekrishnan, Pranab GoswamiBioelectrochemical systems (BESs) have been intensively investigated over the last decade owing to its wide-scale environmentally friendly applications, among which wastewater treatment, power generation and environmental monitoring for pollutants are prominent. Different variants of BES such as microbial fuel cell, microbial electrolysis cell, microbial desalination cell, enzymati...
Source: Journal of Bioscience and Bioengineering - February 8, 2020 Category: Biomedical Science Source Type: research

Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability
Publication date: Available online 6 February 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Karla Ilić Đurđić, Selin Ece, Raluca Ostafe, Simon Vogel, Ana Marija Balaž, Stefan Schillberg, Rainer Fischer, Radivoje ProdanovićLignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H2O2-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we generated a random mutagenesis library c...
Source: Journal of Bioscience and Bioengineering - February 7, 2020 Category: Biomedical Science Source Type: research

Development of a 3D porous chitosan/gelatin liver scaffold for a bioartificial liver device
In this study, a three-dimensional (3D) liver scaffold for in vitro cultures was fabricated by freeze-drying a chitosan/gelatin (CG) solution. A CG scaffold has advantages such as (i) inexpensive and easy-to-make; (ii) easy to fabricate with varying compressive modulus by changing the concentration of glutaraldehyde; (iii) non-cytotoxicity; and (iv) porous structure is similar to extracellular matrix (ECM), thus facilitating hepatocyte adhesion and proliferation. The results revealed that the compressive modulus and maintainability of a CG scaffold was correlated to the increase in glutaraldehyde. Furthermore, hepatocyte ...
Source: Journal of Bioscience and Bioengineering - February 2, 2020 Category: Biomedical Science Source Type: research

Affinity shift of ATP upon glycerol binding to a glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1
Publication date: Available online 31 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Ryuta Hokao, Hiroyoshi Matsumura, Ryota Katsumi, Clement Angkawidjaja, Kazufumi Takano, Shigenori Kanaya, Yuichi KogaGlycerol kinase (GK) is a key enzyme of glycerol metabolism. It participates in glycolysis and lipid membrane biosynthesis. A hexamer of GK from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1(Tk-GK) was identified as a substrate-binding form of the enzyme. Here, the X-ray crystal structure analysis and the biochemical analysis was done and the relationships between its unique oligomer s...
Source: Journal of Bioscience and Bioengineering - January 31, 2020 Category: Biomedical Science Source Type: research

Effect of soil organic matter on petroleum hydrocarbon degradation in diesel/fuel oil-contaminated soil
Publication date: Available online 25 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Yun-An Chen, Pao-Wen Grace Liu, Liang-Ming Whang, Yi-Ju Wu, Sheng-Shung ChengThe purpose of this study is to investigate the effect of soil organic matter (SOM) content levels on the biodegradation of total petroleum hydrocarbons (TPH). Batch experiments were conducted with soils with 2% or 10% organic matter that had been contaminated by diesel or fuel oil. In addition to the TPH (diesel or fuel oil) degradation efficiency, a comprehensive investigation was conducted on the TPH-degrading microbial community using m...
Source: Journal of Bioscience and Bioengineering - January 26, 2020 Category: Biomedical Science Source Type: research

Evaluation of electron-transferring cofactor mediating enzyme systems involved in urolithin dehydroxylation in Gordonibacter urolithinfaciens DSM 27213
Publication date: Available online 23 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Hiroko Watanabe, Shigenobu Kishino, Masatake Kudoh, Hiroaki Yamamoto, Jun OgawaThe gut bacterium Gordonibacter urolithinfaciens DSM 27213 metabolizes ellagic acid into three polyphenol compounds, namely, urolithin M5, urolithin M6, and urolithin C, which are collectively called urolithin. The key reactions of this metabolic pathway are the dehydroxylation of the phenolic hydroxy group, i.e., conversion of urolithin M5 to urolithin M6, and successive conversion of urolithin M6 to urolithin C. By testing the effects o...
Source: Journal of Bioscience and Bioengineering - January 24, 2020 Category: Biomedical Science Source Type: research

Selection and characterization of microalgae with potential for nutrient removal from municipal wastewater and simultaneous lipid production
Publication date: Available online 20 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Tsuyoshi Aketo, Yumiko Hoshikawa, Daisuke Nojima, Yusuke Yabu, Yoshiaki Maeda, Tomoko Yoshino, Hiroyuki Takano, Tsuyoshi TanakaWastewater treatment, along with the simultaneous production of valuable chemical compounds, including lipids by microalgae is a challenging but attractive study. Towards this goal, the candidate microalgae were selected from culture collections or isolated from wastewater in this study. The initial screening test using microalgae revealed that various eukaryotic as well as prokaryotic micro...
Source: Journal of Bioscience and Bioengineering - January 21, 2020 Category: Biomedical Science Source Type: research

Denitrification characteristics and pathways of a facultative anaerobic denitrifying strain, Pseudomonas denitrificans G1
Publication date: Available online 20 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Zhao Chen, Yuli Jiang, Zhiqiang Chang, Jiajia Wang, Xiefa Song, Zhitao Huang, Shibo Chen, Jian LiExcessive nitrate in aquaculture systems has attracted wide attention. To isolate novel aerobic denitrifying strain and characterize its nitrogen removal processes, a facultative anaerobic denitrification bacterium, identified as Pseudomonas denitrificans G1, was isolated from marine sediments. Strain G1 could grow and remove 90–98% of nitrate and 97–99% of nitrite under an aerobic or anaerobic condition in 24 h, wi...
Source: Journal of Bioscience and Bioengineering - January 21, 2020 Category: Biomedical Science Source Type: research

Characterization of a novel marine unicellular alga, Pseudoneochloris sp. strain NKY372003 as a high carbohydrate producer
In this study, the carbohydrate production of Pseudoneochloris sp. strain NKY372003 isolated as a high carbohydrate producer, was investigated. Cultivation conditions with various combinations of nutrient contents and photon flux density were examined to maximize the biomass and carbohydrate productivities. At the optimal condition, the biomass and carbohydrate production of this strain reached 8.11 ± 0.37 g/L and 5.5 ± 0.2 g/L, respectively. As far as we know, this is the highest carbohydrate production by microalgae among ever reported. Cell staining with Lugol's solution visualized intracellular starch granules. Bec...
Source: Journal of Bioscience and Bioengineering - January 12, 2020 Category: Biomedical Science Source Type: research

Outdoor cultivation of Chlorella sp. in an improved thin-film flat-plate photobioreactor in desertification areas
Publication date: Available online 8 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Chenghu Yan, Zhihui Wang, Xia Wu, Shumei Wen, Jing Yu, Wei CongIn order to reduce the flow resistance of the thin-film flat plat photobioreactor (FPPBR) and make it more suitable for mass microalgae cultivation, the channel diameter was modified to 0.06 m and the thin-film FPPBR consisted of 10 parallel shunt-wound channels. A thin-film FPPBR system with 100 modified FPPBRs was constructed and used for Chlorella sp. cultivation in desertification areas (Ordos, China) from July to September of 2018. The pressure drop...
Source: Journal of Bioscience and Bioengineering - January 9, 2020 Category: Biomedical Science Source Type: research

Enhanced bioactivity of Zanthoxylum schinifolium fermented extract: Anti-inflammatory, anti-bacterial, and anti-melanogenic activity
This study intends to assess the potential of fermented Zanthoxylum schinifolium extract for use in biomedical applications. Probiotic bacteria, Lactobacillus rhamnosus A6-5, were deployed as a seed culture for fermentation. The fermented extract showed greater tyrosinase inhibitory activity and reduced melanin production (58.3%) compared with the raw extract. Cytotoxicity assay inferred that 500 μg/mL is the ideal non-toxic concentration with maximum cell viability. In addition, DAPI staining did not show any damage to the chromatin structure of the cells. The anti-aging property of the fermented extract was confirmed b...
Source: Journal of Bioscience and Bioengineering - January 9, 2020 Category: Biomedical Science Source Type: research