Evaluation of semi-automated cells counting in peritoneal fluid
Conclusion:The results suggest that peritoneal fluid should continue to be analyzed in Fuchs-Rosenthal chamber. However, further studies should be conducted with a greater number of samples to investigate the possibility of using automated cells counting in serous fluids and, thus, provide greater speed and quality of results.RESUMOIntrodução:Atualmente, a análise citológica de líquidos biológicos, como líquido peritoneal, é realizada por meio da contagem manual de células, em câmara de Fuchs-Rosenthal. Porém, esse método apresenta uma s&eac...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 16, 2015 Category: Pathology Source Type: research

Performance analysis of software for identification of intestinal parasites
Conclusions:This work contributes to the computerization of healthcare area, and benefits both health professionals and their patients, in addition to provide a more efficient, accurate and secure diagnosis.RESUMOIntrodução:As parasitoses intestinais figuram entre os diagnósticos mais frequentes no mundo. Um diagnóstico clínico acurado das infecções parasitárias humanas depende da confirmação laboratorial para diferenciação específica do agente infeccioso.Objetivos:Criar formas tecnológicas para auxiliar no diagnóstico ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 16, 2015 Category: Pathology Source Type: research

Relationship between the prevalence of hemoglobin S and the ethnic background of blood donors in Paraná state
Conclusion:This study showed higher rates of sickle cell trait in Afro Brazilians, which corroborates data published in other Brazilian regions and states.RESUMOIntrodução:A hemoglobina S (HbS) é uma das alterações hematológicas hereditárias de maior frequência na espécie humana. No Brasil, a anemia falciforme (AF) tem importância epidemiológica significativa em virtude da prevalência e da morbimortalidade que apresenta e por isso tem sido apontada como uma questão de saúde pública.Objetivo:Determinar a prevalência d...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 16, 2015 Category: Pathology Source Type: research

Underreporting of scientific knowledge: a theme for reflection
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 16, 2015 Category: Pathology Source Type: research

Molecular biology techniques for loss of heterozygosity detection: the glioma example
ABSTRACT In recent years, many oncology institutions have implemented the use of molecular approaches to assess and manage cancer patients. One commonly observed type of genetic alteration in cancer is the loss of heterozygosity (LOH). In the clinical setting, this molecular genetic marker is an important tool for disease prognosis, diagnosis and treatment. For example, the loss of 1p/19q is a classical molecular marker for oligodendroglioma assessment. In addition, this marker is associated with a favorable prognosis and chemosensitivity in oligodendroglial tumors. Interpretation of the clinical significance of molecular ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Access to the tracheal pulmonary pathway in small rodents
ABSTRACT The tracheal pulmonary route is used in diverse experimental models for the study of drugs, infectious agents, and diseases. In view of its importance and associated difficulties, the present article proposes to give research groups up-to-date information on techniques to access the tracheal pulmonary pathway of small rodents.RESUMO As vias de acesso traqueopulmonar vêm sendo utilizadas em diversos modelos experimentais que estudam a ação de fármacos e agentes infecciosos, além de enfermidades. Tendo em vista a sua importância e as dificuldades associadas, o presente artigo ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Fatal complication after transsphenoidal surgery of pituitary adenoma: case report
The objective of this study was to report a rare fatal complication in the postoperative period of transsphenoidal surgery of the pituitary gland (adenoma), with a brief review of the subject. The patient was a 54-year-old white man with acromegaly and severe heart failure, who after microsurgery developed blood pressure instability within 32 hours after the procedure and died. The autopsy revealed: hypertrophy and ventricular dilation with myocarditis, pericarditis and myocardial fibrosis; mesenteric ischemia with transmural coagulation necrosis of the intestinal loops; acute tubular necrosis; and hepatic steatosis. The f...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Multicentric angiomyolipoma in kidney, liver, and lymph node: case report/review of the literature
Conclusion: We described a rare AML with renal, hepatic and lymph node involvement, representing a multicenter version instead of metastasis. RESUMO Introdução: O angiomiolipoma (AML) constitui-se de tecido adiposo, vasos sanguíneos e fibras musculares lisas; tem curso clínico benigno, crescimento lento e ausência de metástases, ocorrendo no complexo esclerose tuberosa ou esporadicamente. Objetivos: Objetivamos descrever aspectos clinicorradiológicos e histológicos desse tumor Relato do caso: Paciente do sexo feminino, 64 anos, com dor abdominal e dispepsia. Tomografia...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Detection and genotyping of HPV in women with indeterminate cytology and low-grade squamous intraepithelial lesions
Conclusion: The presence of high-risk HPV in women with old cervical lesions, whether they have evolved or not, indicates the need to improve patient monitoring and surveillance. RESUMO Introdução: O papilomavírus humano (HPV) é o principal fator de risco relacionado com câncer cervical. A identificação precoce de tipos de HPV de alto risco em cérvice normal ou com lesões precursoras pode auxiliar a prevenir que essas lesões progridam para neoplasias malignas. Objetivos: Este estudo teve como objetivo detectar e genotipar o HPV em mulheres com resultado c...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Molecular characterization of Enterobacteriaceae resistant to carbapenem antimicrobials
ABSTRACT The present study aimed to genotypically and phenotypically characterize clinical isolates of carbapenem-resistant Enterobacteriaceae collected from inpatients at the University Hospital of Santa Maria, during seven months. Among the clinical isolates subjected to the modified Hodge test (MHT), 62.5% were positive, indicating possible production of carbapenemase. Polymerase chain reaction (PCR) demonstrated that blaKPC was the most frequently found gene (31%), followed by blaIMP (12.5%). Combined use of the methods is needed to identify carbapenem resistance in enterobacteria to prevent their spread and control th...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Candida species distribution and fluconazole susceptibility of blood isolates at a regional hospital in Passo Fundo, RS, Brazil
Conclusion: The high mortality and fluconazole resistance rates emphasize the importance of the diagnosis of candidemia in a hospital environment. RESUMO Introdução: Candidemia é a infecção da corrente sanguínea produzida por leveduras do gênero Candida. Objetivos: Este estudo teve por objetivo caracterizar a epidemiologia e o perfil de suscetibilidade ao fluconazol em espécies de Candida, isoladas em pacientes internados em um hospital regional de Passo Fundo, RS. Métodos: Registros laboratoriais foram utilizados para identificar pacientes com hemocultura posit...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Influence of hemodialysis on the plasma concentration of adenosine deaminase in patients with chronic kidney disease
The objective of this study was to evaluate biomarkers of renal function and serum ADA of hemodialysis patients. Materials and methods: Blood samples were collected from 80 patients – 40 women and 40 men – between 19 and 60 years old, before and after the completion of hemodialysis. Results: There was a significant difference in levels of creatinine, urea and ADA in pre- and post-hemodialysis periods (p
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Methicillin- and vancomycin-resistant Staphylococcus aureus in health care workers and medical devices
Conclusion: Our results show a higher frequency of MSSA and MRCoNS among S. aureus and CoNS respectively, colonizing devices and health professionals. However, the already described transfer of the staphylococcal cassette chromosome mec (SSCmec) from MRCoNS to MSSA may alter these results, increasing the frequency of MRSA strains. RESUMO Introdução: A contaminação cruzada por Staphylococcus aureus entre pacientes, profissionais e materiais de uso médico em unidades de saúde é uma preocupação constante, o que leva pesquisadores a estudar a prevalência dess...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Identification of polymorphisms of XRCC1 gene in patients with cancer in a city of northern Brazil
Conclusion: Our results demonstrated the allele frequency of 194Trp polymorphism in both sample groups analyzed. We also found a significant number of polymorphic allele 399A in people with cancer. Thus, we can highlight 399Gln polymorphism as a genetic marker of cancer risk in this population. RESUMO Introdução: O câncer é considerado uma doença genética, por isso identificar e caracterizar os genes envolvidos em sua origem e progressão é fundamental para compreender suas bases moleculares. Objetivos: Nosso objetivo foi verificar se os indivíduos de Macap&aacut...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Why hold a congress of Laboratory Medicine in Brazil?
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - July 31, 2015 Category: Pathology Source Type: research

Pulmonary histoplasmosis simulating metastasis: two cases report and review of the literature
Conclusion: In patients with cancer, clinical and radiological findings are not limited to metastatic dissemination of tumors, requiring histopathological confirmation of lesions suggestive of metastasis, excluding other diseases and preventing inappropriate treatment. Introdução e objetivos: Micoses sistêmicas assumem grande importância nas situações de imunodepressão. A histoplasmose pode produzir diversas manifestações clínicas, podendo simular metástases. Objetivamos demonstrar a importância da confirmação histopatológ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Inflammatory fibroid polyp (Vanek’s polyp): a case report and literature review
Inflammatory fibroid polyp (IFP) is a benign uncommon lesion (1%-4% of gastric benign lesions), originated from the submucosa of the gastrointestinal tract. Its origin is controversial and immunohistochemical studies of lesions have largely refuted the possible vascular, neural or smooth muscle origin. Recent studies suggest a neoplastic etiology due to a mutation, in some cases, in the alpha-type platelet-derived growth factor receptor gene (PDGFRa). Our objective is to report the case of a patient aged 70 years, with gastric IFP, comparing her immunohistochemical profile with those of other studies, and a brief review of...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Renal myxoma: a case report
This article reports a case of renal myxoma in an elderly woman with recurrent cystitis. After five years of follow-up, the computed tomography (CT) revealed a large solid tumor mass in the left kidney. Tumor resection was performed preserving the affected kidney with histopathological diagnosis of renal myxoma. The objective of this study is to report a rare case of renal myxoma, emphasizing the importance of the differential diagnosis from other benign and malignant mesenchymal tumors.Mixomas são tumores raros que podem ser encontrados em muitas localizações anatômicas. Na literatura, há...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Histological identification of H. pylori stained by hematoxylin-eosin and Giemsa: review for quality control
Introduction: Several special staining methods are available for H. pylori (Hp) identification in histological sections of chronic gastritis (CG), including the routine hematoxylin-eosin (HE) method. Some reports suggest that ancillary stains are not always needed to establish the diagnosis of Hp infection. In addition, the benefit of using them, when biopsies show minimal inflammation, is not clear. Objective: We performed a retrospective study to compare the usefulness of HE with Giemsa method for the histopathological diagnosis of Hp in tissue sections. Methods: Histological sections from 390 consecutive patients were r...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Quality in cytopathology: an analysis of the internal quality monitoring indicators of the Instituto Nacional de Câncer
Conclusion: The results show that an Internal Quality Monitoring Program is essencial to ensure quality for cytopathology laboratories, and a randomized review of at least 10% of the negative exams, as recommended by the Brazilian Ministry of Health/Instituto Nacional de Câncer (INCA), since is an effective method, especially for large laboratories. Introdução: A estruturação de um sistema de controle de qualidade é necessária para garantir a efetividade do exame preventivo ginecológico, que permanece como principal estratégia para controle do câncer do co...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Absence of atypical haplotype and presence of Senegal haplotype sickle cell disease in African-descent population in the northern Brazil
Conclusion: We identified three haplotypes characteristic of African ethnicity, with the presence of Senegal. In our study we found the presence of atypical haplotype, suggesting concentration and semi-isolation of the founding groups with little mixing. Introdução: A anemia falciforme (AF) é a forma mais grave da doença falciforme; apresenta formas variantes que são chamadas de haplótipos βS. Existem cinco principais haplótipos desse gene: árabe-indiano/saudi, Senegal, Benin, Bantu e Camarões. Objetivos: Caracterizar a presença dos haplótipo...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Blood collection on filter paper for HIV antibodies detection: experience of SampaCentro Project
Conclusion: Blood collection on DBS is feasible for the study of MSM population and is suitable for laboratory routine. The overall performance of Q Preven HIV-1 + 2 - DBS kit was satisfactory, having reached the quality levels required for the development of this study. Introdução: As amostras de sangue colhidas em papel filtro (DBS) têm sido utilizadas na triagem de anticorpos por meio de imunoensaios. Objetivos: Avaliar a estratégia de colheita de sangue em DBS para detecção de anticorpos contra o vírus da imunodeficiência humana (HIV), verificar o lote do kit Q-Prev...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Hematological parameters in children with Down syndrome
This study aimed to analyze the results of CBC performed in children with DS. Patients and methods: Data from CBC of DS children were included; at the time of examination they were aged between 2 and 10 years and had no clinical signs and/or symptoms of infectious disease. The hematological parameters analyzed were: total number of erythrocytes (RBC), hemoglobin (Hb) concentration, hematological indices, platelet count, and total number of leucocytes. Additionally, we compared the collected parameters according to gender and age of the children studied. Results: A total of 203 CBC (100 girls and 103 boys) were evaluated. I...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease
Conclusion: The medical advancement, the development of new techniques and hematological parameters have revealed important information about functional integrity of bone marrow, diagnosis of anemia and recombinant human erythropoietin monitoring therapy used in hemodialysis patients. Introdução: A importância dos valores de referências locais tem sido bastante descrita na literatura, isso porque características da população podem influenciar os testes laboratoriais. Objetivos: Estabelecer o intervalo de referência para parâmetros eritrocitários tradicionais...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Frequency of acute myeloid leukemia in children attended in Belém, Pará from August 2005 to May 2009
Conclusion: In the pediatric population, the types of AML M2, M0/M1 and M3 were respectively the most frequent. Introdução: A leucemia mieloide aguda (LMA) tem incidência variável nas diferentes regiões do Brasil. Objetivos: Determinar a frequência dos subtipos de LMA em crianças entre 0-17 anos, atendidas em Belém, Pará, no período de agosto de 2005 a maio de 2009. Casuística e métodos: Estudo retrospectivo com 278 pacientes com diagnóstico de leucemias agudas ou crônicas com base nos critérios clínicos, morfol&oac...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Detection of H. pylori in endoscopic gastric biopsies: a routine research that goes far beyond the laboratory limits
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - June 13, 2015 Category: Pathology Source Type: research

Accuracy of intraoperative consultation in lesions of the salivary glands: analysis of 748 cases
Conclusion: Our results indicate that IOC in salivary gland lesions is highly accurate and can contribute to the surgical approach. Introdução: As lesões das glândulas salivares são incomuns, representando de 2% a 6,5% de todas as neoplasias da região da cabeça e do pescoço. Devido à diferença de tratamento entre elas, é fundamental um diagnóstico preciso. O estudo citológico por meio de punção aspirativa por agulha fina (PAAF) é um método com alta acurácia utilizado para o diagnóstico das les...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Giant retroperitoneal myelolipoma: case report and literature review
Adrenal myelolipomas are unusual benign tumors with an average age of 60 years at onset, often associated with adrenal gland. A 63-year-old female presenting with abdominal discomfort and a large expanding mass in retroperitoneum occupying the right hemiabdomen, with extrinsic compression of adjacent organs, underwent tumor resection. Macroscopic examination of the surgical specimen showed a large yellowish homogeneous lesion with areas of hemorrhage, covered by a thin fibrous capsule. Microscopic analysis revealed a neoplasm composed of mature adipocytes permeated by hematopoietic tissue. There was residual adrenal cortex...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Cutaneous epithelioid hemangioma of atypical location: a case report
We report a case of epithelioid hemangioma characterized by skin lesion in the left upper limb. This is an unusual location, since the lesions occur most often in the head and neck.O hemangioma epitelioide, também denominado hiperplasia angiolinfoide com eosinofilia, é um tumor vascular raro, de caráter benigno, que se manifesta como nódulos. O exame histopatológico evidencia espaços vasculares de diversos calibres, revestidos por endotélio proeminente e infiltrado inflamatório composto de eosinófilos, histiócitos, mastócitos e linfócitos. ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Renal mixed epithelial and stromal tumor: case report
Mixed epithelial and stromal tumor (MEST) represents a recently described biphasic kidney neoplasm, which predominantly affects perimenopausal females. The authors report the case of a young male patient with a MEST exhibiting positivity for estrogen and progesterone receptors. Computed tomography/magnetic resonance imaging (CT/MRI) showed an expansive lesion affecting the right kidney. Grossly, a solid-cystic tumor was identified, which measured 5.7 × 3.5 × 2.4 cm. On microscopic examination, a biphasic tumor constituted by stromal and epithelial elements, without significant atypias, was identified. The strom...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Preliminary results of Lean method implementation in a pathology lab from Northeastern Brazil
Oncologic care shows a growing and unmet demand, and requires the search for alternatives that allow the efficient use of limited resources, the building of autonomy, and the endeavour for continuous improvement of processes. In the present work, we present the implementation of Lean philosophy at a pathology laboratory of an oncology hospital. Among the preliminary results, we highlight the redefinition of the dynamics of the staff, and the physical reorganization of the area. Such important changes culminated in an expressive reduction of lead time, even with a significant increase in the monthly load of exams.A assist&e...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Prevalence variation of colpocytological abnormalities in adolescents and adults in the years 2000 and 2010
Conclusion: It was observed that the frequency of cervical epithelial abnormalities was higher among adolescents than adults and increased between 2000 and 2010. Introdução: O câncer do colo do útero é o segundo tipo de câncer mais comum entre as mulheres em todo o mundo, e a frequência de lesões pré-neoplásicas vem aumentando entre as adolescentes. Objetivos: Estudar e comparar a frequência de anormalidades epiteliais em exames colpocitológicos em adolescentes e adultas. Materiais e métodos: Estudo retrospectivo com base na análi...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

RDW as differential parameter between microcytic anemias in "pure" and concomitant forms
The objective of this study was to investigate the discriminatory power of red cell distribution width (RDW) between iron deficiency anemia and thalassemia minor. Method: Blood parameters were compared in 227 patients with iron deficiency anemia and/or thalassemia minor after diagnosis confirmed by molecular biology and HbA2 measurement for alpha thalassemia and beta thalassemia trait respectively. The frequency of alpha thalassemia trait in a population from two public hospitals of Minas Gerais was also determined. Result and conclusion: RDW was able to differentiate iron deficiency anemia from thalassemia trait, what ind...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Epidemiological analysis of serological markers of hepatitis B in HIV+ patients from Curitiba and metropolitan region
Conclusion: The findings of the present study corroborate the need to investigate systematically the presence of markers for HBV in HIV+ patients from different regions of the country. Introdução: A presença do vírus da hepatite B (HBV) em indivíduos infectados pelo vírus da imunodeficiência humana (HIV) está associada ao significante aumento da morbimortalidade nos pacientes afetados. Objetivo: Estabelecer a prevalência de marcadores sorológicos de HBV e dos fatores epidemiológicos associados em pacientes HIV+ acompanhados pelo Hospital de Cl&iacut...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Level of reactivity of IgM anticardiolipin antibodies in preeclampsia: a likely early serologic marker
The purpose of this study was to measure the level of anticardiolipin antibodies in preeclamptic pregnant women to verify a possible association between the presence of these antibodies and the development of the syndrome. A total of 36 pregnant women with preeclampsia and 19 women with normal pregnancy were evaluated. Anticardiolipin antibodies were determined by enzyme immunoassay. The reactivity level of anticardiolipin antibodies was significantly higher in the preeclamptic group, with a significance level of 95%. The results can mean the participation of anticardiolipin antibodies in the pathogenesis of preeclampsia. ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

The importance of immunophenotyping by flow cytometry in distinction between hematogones and B lymphoblasts
This article presents information about the morphological and immunophenotypic characteristics of B lymphoid precursors and verifies the relevance of immunophenotyping by flow cytometry (FC) in the distinction between those cells. This differentiation is essential to establish a correct prognosis and assist in medical decision about the most appropriate therapeutic scheme.As hematogônias são precursores normais de linhagem linfoide B da medula óssea. Os linfoblastos B representam células imaturas neoplásicas presentes em pacientes portadores de leucemia linfoblástica aguda de c&eacu...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Accuracy of intraoperative consultation in the evaluation of salivary gland tumors
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 22, 2015 Category: Pathology Source Type: research

Absence of Wharton's jelly: case report
There are few reports in the literature of the absence of Wharton's Jelly. Here we report the seventh case in a primigravida, 22 years old, admitted after vaginal delivery of stillborn. The umbilical cord have a long segment with disruption of cord structures and the three blood vessels were completely separated from each other, with a minimum amount of Wharton's jelly remaining around each vessel. The absence of Wharton' jelly is associated with fetal distress, intrauterine growth restriction, and fetal death. Quantitative/qualitative studies of Wharton's jelly represent an open field of research for possible correlations...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Impact of using a lymph node revealing solution in surgical specimens for pathological staging of gastric cancer
Conclusion: The use of LRS in gastrectomy specimens is simple, enables dissection, increases the number of LNs, and occasionally changes pN staging and stage grouping. Introdução: O envolvimento do linfonodo (LNs) por metástase é importante critério para estadiar câncer gástrico (CG). Relaciona-se o prognóstico do CG com número de LNs metastáticos. Objetivos: Avaliar o impacto do uso de solução reveladora de linfonodos (SRL) em espécimes de gastrectomia. Materiais e métodos: Estudo prospectivo em 30 espécimes de gastrect...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Influence of external factors on the preservation of human nervous tissue for histological studies: review article
Neuropathological studies are crucial for the new knowledge on pathophysiology and treatment of neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease. The postmortem brain tissue processing method directly impacts on both the appropriate integrity and the biomolecules detection by different histological and molecular biology techniques. In this review we will discuss topics on the influence of some external factors on the preservation of the brain tissue for histological studies (histochemistry and immunohistochemistry), such as factors either prior or after the death, and the chosen method for th...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Infection by multidrug-resistant Elizabethkingia meningoseptica: case reports
We report two cases of sepsis in critically ill patients in two tertiary care hospitals in Recife-PE, Brazil. The first case is an 87-year-old patient with chronic myeloid leukemia and sepsis; and the second case is a 93-year-old patient with prostate cancer and septic shock caused by multidrug-resistant (MDR) Elizabethkingia meningoseptica.Reportamos dois casos de sepse em pacientes criticamente debilitados em dois hospitais com nível de complexidade terciária em Recife-PE, Brasil. O primeiro caso, paciente de 87 anos com leucemia mieloide crônica e sepse; o segundo, paciente com 93 anos de idade com c...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Markers of acute-phase response in the treatment of pulmonary tuberculosis
Introduction: Tuberculosis promotes an acute phase response with an increase of blood reactants, such as C-reactive protein (CRP), among others, which are associated with increased erythrocyte sedimentation rate (ESR). Objective: Evaluate the ESR and the CRP as markers for diagnosis and monitoring cases of pulmonary tuberculosis. Method: Research on patients with clinical, laboratory, and imaging diagnosis of pulmonary tuberculosis, from Itajaí-SC; in which CRP and ESR were analyzed in three different times: at diagnosis, before starting treatment (T0), after three months of treatment (T1), and at the end of treatme...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Microbial resistance and frequency of extended-spectrum beta-lactamase (ESBL) in isolated from blood cultures
Conclusion: The increase in the occurrence of ESBL in different Enterobacteriaceae shows the importance of the amplification of detection in other species than Escherichia coli or Klebsiella sp., so that the assistance to the patient is not restrained, since these resistant bacteria cannot be detected by the laboratories. Considering the frequency of ESBL in this study, we highlight the importance of its detection, aiming to its contribution to the development of improvements in the health care policies of hospitals. Introdução: A emergência e a disseminação dos isolados portadores de beta...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Research on antimicrobial residues activity in urine samples of hospitalized patients
Conclusion: Automated technique showed better performance when compared to manual technique, with sensitivity of 92.8% and 71.4%, respectively. The presence of antimicrobial residues may affect the recovery of bacteria in the urine, producing a false-negative result. Introdução: A infecção do trato urinário é bastante frequente em ambiente hospitalar, e a cultura de urina é padrão-ouro para o diagnóstico dessa doença, pois permite a identificação bacteriana e a realização do teste de suscetibilidade aos antimicrobianos. Amostr...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Prevalence of unnecessary laboratory tests and related avoidable costs in intensive care unit
Conclusion: Significant number of unnecessary testing was found, with occurrence pattern on Monday and not dependent on age or length of stay. The data indicate the need to implement guidelines or protocols for ordering laboratory tests in the ICU, which have proven effective in helping the prescribing professional in their clinical practice, and contribute to optimize for health care spending. Introdução: Com o aumento dos recursos diagnósticos, os exames laboratoriais têm se tornado ferramenta indispensável nas elucidações diagnósticas. Observa-se, portanto, um cresc...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension
Conclusion: The validated HPLC method showed good performance for quantification of plasma homocysteine levels, while S-Ec method provided results for homocysteine comparable with those obtained by the validated method; therefore, this methodology is a potential alternative of automated method for clinical laboratories. Introdução e objetivo: A determinação dos níveis plasmáticos de homocisteína tem sido relatada como um marcador de risco de interesse em doenças graves que cursam com lesões endoteliais, estando associada ao desenvolvimento ou à progress&...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Prevalence of discordant microscopic changes with automated CBC analysis
Conclusion: It was observed that it is of fundamental importance that the microscopy qualitative analysis must be performed in parallel with automated analysis in order to obtain reliable results, causing a positive impact on the prevention, diagnosis, prognosis, and therapeutic follow-up. Introdução: A causa mais comum de erro no diagnóstico relaciona-se com erros de exames laboratoriais, bem como erros de sua interpretação. E com o intuito de reduzi-los, atualmente o laboratório dispõe de equipamentos modernos com resultados precisos e confiáveis. O desenvolvimento ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

The importance and usefulness of terminology and nomenclature for Cytopathology
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 1, 2015 Category: Pathology Source Type: research

Unusual combination of gestational trophoblastic neoplasias: case report
We present a rare case of coexistence of choriocarcinoma and placental-site trophoblastic tumor in the same tumor, whose diagnosis was made based on the correlation of morphological, microscopic and immunocytochemical studies, due to the difficulty in diagnosing these mixed tumors based on conventional histology only.A doença trofoblástica gestacional compreende um grupo heterogêneo de lesões decorrentes da proliferação anormal de células trofoblásticas. A elevação da subunidade beta da gonadotrofina coriônica humana (β-hCG) após a evacu...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 11, 2015 Category: Pathology Source Type: research

Abrikossoff’s tumor: report of a rare tumor and its unusual vulvar location
The objective of this paper is to describe a case of granular cell tumor located in the vulva.O primeiro relato do tumor de células granulosas ocorreu em 1926, por Abrikossoff, nome que deu origem à denominação “tumor de Abrikossoff”. Trata-se de neoplasia benigna, incomum, observada mais frequentemente na cabeça, no pescoço e na língua, sendo encontrado na vulva em apenas 6%-7% dos casos. Sua histogênese é incerta, provavelmente ligada às células de Schwann. O tratamento é cirúrgico, com bom prognóstico. Podem ocor...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 11, 2015 Category: Pathology Source Type: research