Where are all the anthelmintics? Challenges and opportunities on the path to new anthelmintics
Publication date: Available online 29 July 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Samantha A. Nixon, Claudia Welz, Debra Woods, Livio Costa-Junior, Mostafa Zamanian, Richard Martin (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - July 31, 2020 Category: Parasitology Source Type: research

A conserved coccidian gene is involved in Toxoplasma sensitivity to the anti-apicomplexan compound, tartrolon E
Publication date: Available online 25 July 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Gregory D. Bowden, Patricia M. Reis, Maxwell B. Rogers, Rachel M. Bone Relat, Kelly A. Brayton, Sarah K. Wilson, Bruno Martorelli Di Genova, Laura J. Knoll, Felix J. Nepveux V, Albert K. Tai, Timothy R. Ramadhar, Jon Clardy, Roberta M. O'Connor (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - July 26, 2020 Category: Parasitology Source Type: research

Energy blackout against glycolytic parasites
Publication date: Available online 22 July 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Alba Martínez-Flórez, Melina Galizzi, Luis Izquierdo, Juan M. Bustamante, Fernando Rodriguez, Alhelí Rodríguez-Cortés, Jordi Alberola (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - July 24, 2020 Category: Parasitology Source Type: research

Drug repurposing applied: Activity of the anti-malarial mefloquine against Echinococcus multilocularis
Publication date: Available online 2 July 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Britta Lundström-Stadelmann, Reto Rufener, Andrew Hemphill (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - July 3, 2020 Category: Parasitology Source Type: research

Mode of action of a formulation containing hydrazones and saponins against leishmania spp. Role in mitochondria, proteases and reinfection process
Publication date: Available online 26 June 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Yulieth A. Upegui Zapata, Fernando Echeverri, Winston Quiñones, Fernando Torres, Montserrat Nacher, Luis I. Rivas, Camila dos Santos Meira, Lashitew Gedamu, Gustavo Escobar, Rosendo Archbold, Iván D. Vélez, Sara M. Robledo (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 28, 2020 Category: Parasitology Source Type: research

Repurposing the open access malaria box reveals compounds with activity against Tritrichomonas foetus trophozoites
Publication date: Available online 17 June 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Katy A. Martin, Jeba R.J. Jesudoss Chelladurai, Christopher Bader, Elizabeth Carreiro, Katelyn Long, Kylie Thompson, Matthew T. Brewer (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 18, 2020 Category: Parasitology Source Type: research

Novel structural CYP51 mutation in Trypanosoma cruzi associated with multidrug resistance to CYP51 inhibitors and reduced infectivity
Publication date: Available online 13 June 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Caio H. Franco, David C. Warhurst, Tapan Bhattacharyya, Ho Y.A. Au, Hai Le, Miriam A. Giardini, Bruno S. Pascoalino, Ana Claudia Torrecilhas, Lavinia M.D. Romera, Rafael Pedro Madeira, Sergio Schenkman, Lucio H. Freitas-Junior, Eric Chatelain, Michael A. Miles, Carolina B. Moraes (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 14, 2020 Category: Parasitology Source Type: research

In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
Publication date: Available online 2 June 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Lenu B. Fakae, Carl W. Stevenson, Xing-Quan Zhu, Hany M. Elsheikha (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 3, 2020 Category: Parasitology Source Type: research

Identification of annotated bioactive molecules that impair motility of the blood fluke Schistosoma mansoni
Publication date: Available online 1 June 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Thomas B. Duguet, Anastasia Glebov, Asimah Hussain, Shashank Kulkarni, Igor Mochalkin, Timothy G. Geary, Mohammed Rashid, Thomas Spangenberg, Paula Ribeiro (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 1, 2020 Category: Parasitology Source Type: research

Naphthylisoquinoline alkaloids, validated as hit multistage antiplasmodial natural products
Publication date: Available online 24 May 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Phanankosi Moyo, William Shamburger, Mariëtte E. van der Watt, Janette Reader, Ana Carolina C. de Sousa, Timothy J. Egan, Vinesh J. Maharaj, Gerhard Bringmann, Lyn-Marie Birkholtz (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - May 25, 2020 Category: Parasitology Source Type: research

Dicyclanil resistance in the australian sheep blowfly, Lucilia cuprina, substantially reduces flystrike protection by dicyclanil and cyromazine based products
Publication date: Available online 11 May 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Narelle Sales, Monica Suann, Kim Koeford (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - May 11, 2020 Category: Parasitology Source Type: research

Impact of clinically acquired miltefosine resistance by Leishmania infantum on mouse and sand fly infection
Publication date: Available online 1 May 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Lieselotte Van Bockstal, Dimitri Bulté, Sarah Hendrickx, Jovana Sadlova, Petr Volf, Louis Maes, Guy Caljon (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - May 2, 2020 Category: Parasitology Source Type: research

Cholinergic receptors on intestine cells of Ascaris suum and activation of nAChRs by levamisole
Publication date: Available online 25 April 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Mark McHugh, Paul Williams, Saurabh Verma, Jo Anne Powell-Coffman, Alan P. Robertson, Richard J. Martin (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - April 29, 2020 Category: Parasitology Source Type: research

Efficacy evaluation of anthelmintic products against an infection with the canine hookworm (Ancylostoma caninum) isolate Worthy 4.1F3P in dogs
Publication date: Available online 20 April 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Pablo D. Jimenez Castro, Abdelmoneim Mansour, Samuel Charles, Joe Hostetler, Terry Settje, Daniel Kulke, Ray M. Kaplan (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - April 22, 2020 Category: Parasitology Source Type: research

Why does oxamniquine kill Schistosoma mansoni and not S. haematobium and S. japonicum?
Publication date: Available online 10 April 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Anastasia R. Rugel, Meghan A. Guzman, Alexander B. Taylor, Frédéric D. Chevalier, Reid S. Tarpley, Stanton F. McHardy, Xiaohang Cao, Stephen P. Holloway, Timothy J.C. Anderson, P. John Hart, Philip T. LoVerde (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - April 10, 2020 Category: Parasitology Source Type: research

MRPA-independent mechanisms of antimony resistance in Leishmania infantum
Publication date: Available online 2 April 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Noélie Douanne, Victoria Wagner, Gaetan Roy, Philippe Leprohon, Marc Ouellette, Christopher Fernandez-Prada (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - April 3, 2020 Category: Parasitology Source Type: research

Schistosome TRP channels: an appraisal
Publication date: Available online 24 March 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Swarna Bais, Robert M. Greenberg (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - March 25, 2020 Category: Parasitology Source Type: research

Drug resistance in liver flukes
Publication date: April 2020Source: International Journal for Parasitology: Drugs and Drug Resistance, Volume 12Author(s): I. Fairweather, G.P. Brennan, R.E.B. Hanna, M.W. Robinson, P.J. Skuce (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - March 14, 2020 Category: Parasitology Source Type: research

An image-based Pathogen Box screen identifies new compounds with anti-Giardia activity and highlights the importance of assay choice in phenotypic drug discovery
Publication date: Available online 13 March 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Snigdha Tiash, Jake Saunders, Christopher J.S. Hart, John H. Ryan, Andrew G. Riches, Tina S. Skinner-Adams (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - March 13, 2020 Category: Parasitology Source Type: research

Large scale screening for benzimidazole resistance mutations in Nematodirus battus, using both pyrosequence genotyping and deep amplicon sequencing, indicates the early emergence of resistance on UK sheep farms.
Publication date: Available online 6 March 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Lynsey Melville, Elizabeth Redman, Alison A. Morrison, Pai Chia Rebecca Chen, Russell Avramenko, Sian Mitchell, Jan Van Dijk, Giles Innocent, Fiona Sargison, Catriona Aitken, John S. Gilleard, Dave J. Bartley (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - March 7, 2020 Category: Parasitology Source Type: research

Evaluation of a plant-based food supplement to control flea populations in dogs: a prospective double-blind randomized study
Publication date: Available online 15 February 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Fabien MOOG, Gaetan Vetea PLICHART, B.L.U.A. Jean-Louis, C.A.D.I.E.R.G.U.E.S. Marie-ChristineAbstractA prospective double-blind randomized placebo-controlled study evaluated the tolerance and efficacy of the biological plant-based food supplement Bioticks® (extracts of thyme, rosemary, melissa, fenugreek, absinthe and lemongrass) as a flea-control product. Twelve dogs were used as placebo controls (group A). Ten dogs under similar housing conditions received the same food daily but supp...
Source: International Journal for Parasitology: Drugs and Drug Resistance - February 17, 2020 Category: Parasitology Source Type: research

Drug resistance in liver flukes.
Publication date: Available online 10 January 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): I. Fairweather, G.P. Brennan, R.E.B. Hanna, M.W. Robinson, P.J. SkuceAbstractLiver flukes include Fasciola hepatica, Fasciola gigantica, Clonorchis sinensis, Opisthorchis spp., Fascioloides magna, Gigantocotyle explanatum and Dicrocoelium spp. The two main species, F. hepatica and F. gigantica, are major parasites of livestock and infections result in huge economic losses. As with C. sinensis, Opisthorchis spp. and Dicrocoelium spp., they affect millions of people worldwide, causing severe h...
Source: International Journal for Parasitology: Drugs and Drug Resistance - January 10, 2020 Category: Parasitology Source Type: research

Dealing with double trouble: Combination deworming against double-drug resistant cyathostomins
This study consisted of a field study and two computer simulations to evaluate the efficacy of a moxidectin-oxibendazole (MOX-OBZ) combination against the same cyathostomin population. In the field study, anthelmintic treatments occurred when ten horses exceeded 100 eggs per gram. Fecal egg counts and efficacy evaluations were performed every two weeks. The two simulations utilized weather data as well as equine and parasite population parameters from the field study. The first simulation repeated the treatment schedule used in the field study over a 40 year period. The second evaluated efficacies of combination treatments...
Source: International Journal for Parasitology: Drugs and Drug Resistance - December 17, 2019 Category: Parasitology Source Type: research

Current trends in the pharmacological management of Chagas disease
Publication date: Available online 10 December 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Vanessa Ribeiro, Nayra Dias, Taís Paiva, Luciana Hagström-Bex, Nadjar Nitz, Riccardo Pratesi, Mariana HechtAbstractChagas disease (CD) is a tropical neglected illness, affecting mainly populations of low socioeconomic status in Latin America. An estimated 6 to 8 million people worldwide are infected with Trypanosoma cruzi, the etiological agent of CD. Despite being one of the main global health problems, this disease continues without effective treatment during the chronic phase...
Source: International Journal for Parasitology: Drugs and Drug Resistance - December 12, 2019 Category: Parasitology Source Type: research

Short-course quinazoline drug treatments are effective in the Litomosoides sigmodontis and Brugia pahangi jird models
In conclusion, CBR417 or CBR490 are pre-clinical candidates for filarial diseases, which achieve long-term clearance of Wolbachia endosymbionts of filarial nematodes, inhibit filarial embryogenesis and clear microfilaremia with treatments as short as 7 days.Graphical abstract (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - December 10, 2019 Category: Parasitology Source Type: research

Widespread resistance mutations to sulfadoxine-pyrimethamine in malaria parasites imported to China from Central and Western Africa
ConclusionsThese data are cause for great concern and call for continued surveillance of the efficacy of SP in source and recipient populations, and should be considered when developing treatment policy for imported malaria cases in China and elsewhere.Graphical abstract (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 30, 2019 Category: Parasitology Source Type: research

Mutations in the TaPIN1 peptidyl prolyl isomerase gene in Theileria annulata parasites isolated in Sudan
Publication date: Available online 26 November 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Bashir Salim, Elisha Chatanga, Guillaume Jannot, Ehab Mossaad, Ryo Nakao, Jonathan B. WeitzmanAbstractThe tick-borne parasite Theileria annulata is the causative agent of tropical theileriosis or Mediterranean theileriosis. Infection of bovine leukocytes by the obligate intracellular parasites induces proliferative and invasive phenotypes associated with activated signaling pathways. The transformed phenotypes of infected cells are reversible by treatment with the theilericidal drug buparva...
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 27, 2019 Category: Parasitology Source Type: research

Paromomycin is superior to metronidazole in Dientamoeba fragilis treatment
Publication date: Available online 11 November 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Ander Burgaña Agoües, Rosa Abellana, Stanislav Zlatanov Yordanov, Rabee Kazan, A. Mauricio Pérez Ortiz, Cristina Castillo Ramos, Christian Garavito Hernández, Miriam Molina Rivero, Alessandra Queiroga Gonçalves, Emma Padilla, Josefa Pérez, Roger García Puig, Tomas M. Perez-PorcunaABSTRACTDientamoeba fragilis is a trichomonad parasite of the human intestine that is found worldwide. However, the biological cycle and transmission of this parasite ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 12, 2019 Category: Parasitology Source Type: research

Identification of plicamycin, TG02, panobinostat, lestaurtinib, and GDC-0084 as promising compounds for the treatment of central nervous system infections caused by the free-living amebae Naegleria, Acanthamoeba and Balamuthia
Publication date: Available online 22 October 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Monica M. Kangussu-Marcolino, Gretchen M. Ehrenkaufer, Emily Chen, Anjan Debnath, Upinder SinghAbstractThe free-living amebae Naegleria, Acanthamoeba, and Balamuthia cause rare but life-threatening infections. All three parasites can cause meningoencephalitis. Acanthamoeba can also cause chronic keratitis and both Balamuthia and Acanthamoeba can cause skin and systemic infections. There are minimal drug development pipelines for these pathogens despite a lack of available treatment regimens ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 24, 2019 Category: Parasitology Source Type: research

Antileishmanial activity of terpenylquinones on Leishmania infantum and their effects on Leishmania topoisomerase IB
Publication date: Available online 22 October 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Yolanda Pérez-Pertejo, José M. Escudero-Martínez, Rosa M. Reguera, Rafael Balaña-Fouce, Pablo A. García, Pablo G. Jambrina, Arturo San Feliciano, María-Ángeles CastroAbstractLeishmania is the aethiological agent responsible for the visceral leishmaniasis, a serious parasite-borne disease widely spread all over the World. The emergence of resistant strains makes classical treatments less effective; therefore, new and better drugs are necessary....
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 24, 2019 Category: Parasitology Source Type: research

Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta
Publication date: Available online 6 October 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Frank Turnbull, Eileen Devaney, Alison A. Morrison, Roz Laing, Dave J. BartleyAbstractRecent reports of monepantel (MPTL) resistance in UK field isolates of Teladorsagia circumcincta has highlighted the need for a better understanding of the mechanism of MPTL-resistance in order to preserve its anthelmintic efficacy in this economically important species.Nine discrete populations of T. circumcincta were genotypically characterised; three MPTL-susceptible isolates, three experimentally selecte...
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 7, 2019 Category: Parasitology Source Type: research

Profiles of Kelch mutations in Plasmodium falciparum across South Asia and their implications for tracking drug resistance
Publication date: Available online 4 October 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Jyoti Chhibber-Goel, Amit SharmaAbstractArtemisinin-based combination therapy (ACT) offers highly successful treatment of malaria. Emergence and spread of Plasmodium falciparum (Pf) parasites with decreased susceptibility to ACT in South-East Asia has caused concern worldwide. The current accepted criteria to assess artemisinin (ART) resistance relies upon data on treatment failure, delayed parasite clearance at day 3 (DPC3), parasite clearance half-life (PCHL) and in-vitro/ex-vivo ring stage...
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 6, 2019 Category: Parasitology Source Type: research

Development of a topical liposomal formulation of Amphotericin B for the treatment of cutaneous leishmaniasis
Publication date: Available online 23 September 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Mahmoud Reza Jaafari, Mahdi Hatamipour, Seyedeh Hoda Alavizadeh, Azam Abbasi, Zahra Saberi, Sima Rafati, Yasaman Taslimi, Akram Miramin Mohammadi, Ali KhamesipourAbstractBackgroundCurrently, there is no topical treatment available for any form of cutaneous leishmaniasis (CL) in most of the endemic areas. The aim of the current study was to develop a topical nano-liposomal Amphotericin B (AmB) for the treatment of CL.Methodology/Principal Findings: Liposomes containing 0.1, 0.2 and 0.4% AmB...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 24, 2019 Category: Parasitology Source Type: research

Topoisomerase IB poisons induce histone H2A phosphorylation as a response to DNA damage in Leishmania infantum
This report shows for the first time the effect of a series of synthetic indenoisoquinolines on histone H2A phosphorylation, which represents a primary signal of double stranded DNA break in genus Leishmania.Graphical abstract (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 21, 2019 Category: Parasitology Source Type: research

Anti-schistosomal action of the calcium channel agonist FPL-64176
Publication date: Available online 14 September 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Paul McCusker, John D. ChanAbstractSubversion of parasite neuromuscular function is a key strategy for anthelmintic drug development. Schistosome Ca2+ signaling has been an area of particular interest for decades, with a specific focus on L-type voltage-gated Ca2+ channels (Cavs). However, the study of these channels has been technically challenging. One barrier is the lack of pharmacological probes that are active on flatworms, since the dihydropyridine (DHP) based ligands typically used ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 15, 2019 Category: Parasitology Source Type: research

Impaired development of a miltefosine-resistant Leishmania infantum strain in the sand fly vectors Phlebotomus perniciosus and Lutzomyia longipalpis
ConclusionThe MIL resistant L. infantum LEM3323 line is significantly hampered in its development and transmissibility potential in two sand fly vector species. Additional studies are warranted to evaluate whether this applies to other visceral Leishmania parasites with acquired MIL-resistance.Graphical abstract (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 12, 2019 Category: Parasitology Source Type: research

Similarities and differences in the biotransformation and transcriptomic responses of Caenorhabditis elegans and Haemonchus contortus to five different benzimidazole drugs
Publication date: Available online 9 September 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): S.J. Stasiuk, G. MacNevin, M. Workentine, D. Gray, E. Redman, D. Bartley, A. Morrison, N. Sharma, D. Colwell, D.K. Ro, J.S. GilleardAbstractWe have undertaken a detailed analysis of the biotransformation of five of the most therapeutically important benzimidazole anthelmintics - albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), oxfendazole (OxBZ) and fenbendazole (FBZ) - in Caenorhabditis elegans and the ruminant parasite Haemonchus contortus. Drug metabolites were detected by LC-M...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 10, 2019 Category: Parasitology Source Type: research

Prevalence of Plasmodium falciparum Pfcrt and Pfmdr1 alleles in settings with different levels of Plasmodium vivax co-endemicity in Ethiopia
This study thus assessed the prevalence of Pfcrt-K76T and Pfmdr1-N86Y point mutations in P. falciparum. 18S RNA gene based nested PCR confirmed P. falciparum samples (N = 183) collected through community and health facility targeted cross-sectional surveys from settings with varying P. vivax and P. falciparum endemicity were used. The proportion of Plasmodium infections that were P. vivax was 62.2% in Adama, 41.4% in Babile, 30.0% in Benishangul-Gumuz to 6.9% in Gambella. The Pfcrt-76T mutant haplotype was observed more from samples with higher endemicity of P. vivax as being 98.4% (61/62), 100% (31/31), 65.2% (15/23) ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 10, 2019 Category: Parasitology Source Type: research

Successful treatment by adding thalidomide to meglumine antimoniate in a case of refractory anthroponotic mucocutaneous leishmaniasis
Publication date: Available online 27 August 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Vahid Mashayekhi Goyonlo, Sadegh Vahabi-Amlashi, Faezeh TaghaviAbstractMucosal leishmaniasis (ML) is mostly associated with Leishmania braziliensis; however, a few cases of Leishmania tropica induced mucocutaneous leishmaniasis have been reported. The standard treatment for leishmaniasis is pentavalent antimonials, but several other drugs for resistant cases have been proposed including amphotericin and miltefosine. Here we present a case of multiple treatment resistant mucocutaneous leishman...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 3, 2019 Category: Parasitology Source Type: research

Managing anthelmintic resistance in cyathostomin parasites: Investigating the benefits of refugia-based strategies
This study made use of the cyathostomin model to evaluate the influence of treatment strategies with between 1 and 8 yearly treatment occasions, where either 1) all horses were treated, 2) a predetermined proportion of the herd remained untreated, or 3) horses were treated if their FEC exceeded thresholds between 100 and 600 strongyle eggs per gram. Weather data representing four different climatic zones was used and three different herd age structures were compared; 1) all yearlings, 2) all mature horses 10–20 years old, and 3) a mixed age structure of 1–20 years of age. Results indicated a consistent effect o...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 29, 2019 Category: Parasitology Source Type: research

Successful treatment by adding thalidomide to glucantime in a case of refractory anthroponotic mucocutaneous leishmaniasis
Publication date: Available online 27 August 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Vahid Mashayekhi Goyonlo, Sadegh Vahabi-Amlashi, Faezeh TaghaviAbstractMucosal leishmaniasis (ML) is mostly associated with Leishmania braziliensis; however, a few cases of Leishmania tropica induced mucocutaneous leishmaniasis have been reported. The standard treatment for leishmaniasis is pentavalent antimonials, but several other drugs for resistant cases have been proposed including amphotericin and miltefosine. Here we present a case of multiple treatment resistant mucocutaneous leishman...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 28, 2019 Category: Parasitology Source Type: research

Evaluation of synergy between host and pathogen-directed therapies against intracellular Leishmania donovani
Publication date: Available online 21 August 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): M. Shamim Hasan Zahid, Monica M. Johnson, Robert J. Tokarski, Abhay R. Satoskar, James R. Fuchs, Eric M. Bachelder, Kristy M. AinslieAbstractVisceral leishmaniasis (VL) is associated with treatment complications due to the continued growth of resistant parasites toward currently available pathogen-directed therapeutics. To limit the emergence and combat resistant parasites there is a need to develop new anti-leishmanial drugs and alternative treatment approaches, such as host-directed therape...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 22, 2019 Category: Parasitology Source Type: research

In-situ immune profile of polymorphic vs. macular Indian Post Kala-azar Dermal Leishmaniasis
Publication date: Available online 22 August 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Ritika Sengupta, Shibabrata Mukherjee, Srija Moulik, Sneha Mitra, Surya Jyati Chaudhuri, Nilay Kanti Das, Uttara Chatterjee, Mitali ChatterjeeAbstractPost Kala-azar Dermal Leishmaniasis (PKDL), a sequel of apparently cured Visceral Leishmaniasis presents in South Asia with papulonodular (polymorphic) or hypomelanotic lesions (macular). Till date, the polymorphic variant was considered predominant, constituting 85–90%. However, following active-case surveillance, the proportion of macula...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 22, 2019 Category: Parasitology Source Type: research

Development of amplicon sequencing for the analysis of benzimidazole resistance allele frequencies in field populations of gastrointestinal nematodes
This study provides proof of concept of a practical, accurate, sensitive and scalable method to determine frequency of anthelmintic resistance mutations in gastrointestinal nematodes in field studies and as a management tool for livestock farmers.Graphical abstract (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 15, 2019 Category: Parasitology Source Type: research

The combination therapy of meglumine antimoniate and oxiranes (epoxy-α-lapachone and epoxymethyl-lawsone) enhance the leishmanicidal effect in mice infected by Leishmania (Leishmania) amazonensis
In this study we analyzed the potential of the association of meglumine antimoniate (MA) with the oxiranes epoxy-α-lapachone (LAP) or epoxymethyl-lawsone (LAW). Results demonstrated that association between these drugs enhanced leishmanicidal activity on Leishmania (Leishmania) amazonensis infection. The compounds were tested in monotherapy or in combinations (3:1; 1:1 and 1:3) and reduced intracellular parasite numbers, measured by the endocytic index, in all tested conditions. The most effective combination regimens were MA/LAP or MA/LAW in 3:1 ratio, which achieved a reduction of 98.3% and 93.6% in the endocytic i...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 13, 2019 Category: Parasitology Source Type: research

Activity of methylgerambullin from Glycosmis species (Rutaceae) against Entamoeba histolytica and Giardia duodenalis in vitro
Publication date: Available online 10 August 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Mirjana Drinić, Adriane Raninger, Andrea Zraunig, Florian Astelbauer, David Leitsch, Andreas Obwaller, Julia Walochnik, Harald Greger, Michael DuchêneAbstractEntamoeba histolytica and Giardia duodenalis are widespread intestinal protozoan parasites which both spread via cysts that have to be ingested to infect a new host. Their environment, the small intestine for G. duodenalis and the colon for E. histolytica, contains only very limited amounts of oxygen, so both parasites generate en...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 12, 2019 Category: Parasitology Source Type: research

Erratum to ‘Combining bioinformatics, cheminformatics, functional genomics and whole organism approaches for identifying epigenetic drug targets in Schistosoma mansoni’ [IJP: Drugs and Drug Resistance 8 (2018) 559–570]
Publication date: Available online 2 July 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Gilda Padalino, Salvatore Ferla, Andrea Brancale, Iain W. Chalmers, Karl F. Hoffmann (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - July 3, 2019 Category: Parasitology Source Type: research

Route map for the discovery and pre-clinical development of new drugs and treatments for cutaneous leishmaniasis
Publication date: Available online 20 June 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Diana Caridha, Brian Vesely, Katrien van Bocxlaer, Byron Arana, Charlie Mowbray, Sima Rifati, Silvia Uliana, Rosa Regura, Mara Kreishman-Deitrick, Richard Sciotti, Pierre Buffet, Simon L. CroftAbstractAlthough there have been significant advances in the treatment of visceral leishmaniasis (VL) and several novel compounds are currently in pre-clinical and clinical development for this manifestation of leishmaniasis, there have been limited advances in drug research and development (R & D) fo...
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 20, 2019 Category: Parasitology Source Type: research

Screening the Medicines for Malaria Venture Pathogen Box against piroplasm parasites
In this study, the 400 compounds in the Pathogen Box provided by the Medicines for Malaria Venture foundation were screened against Babesia bovis, Babesia bigemina, Babesia caballi, and Theileria equi. A fluorescence-based method using SYBR Green 1 stain was used for initial in vitro screening and determination of the half maximal inhibitory concentration (IC50). The initial in vitro screening performed using a 1 μM concentration as baseline revealed nine effective compounds against four tested parasites. Two “hit” compounds, namely MMV021057 and MMV675968, that showed IC50  100 were selected. The IC50s ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 20, 2019 Category: Parasitology Source Type: research

Novel and safe single-dose treatment of cutaneous leishmaniasis with implantable amphotericin B-Loaded microparticles
Publication date: Available online 17 June 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Ariane J. Sousa-Batista, Wallace Pacienza-Lima, Maria Inês Ré, Bartira Rossi-BergmannAbstractThe development of an effective amphotericin B (AmB) topical formulation to replace the systemically toxic injections currently used in cutaneous leishmaniasis (CL) treatment is challenging due to poor absorption through the skin. Aiming at an effective local chemotherapy, we designed PLGA (poly lactide-co-glycolide acid) microspheres loaded with deoxycholate amphotericin B (d-AmB) for both...
Source: International Journal for Parasitology: Drugs and Drug Resistance - June 18, 2019 Category: Parasitology Source Type: research