A Novel Test for Determination of Wild Felid-Domestic Cat Hybridization
Publication date: Available online 22 October 2019Source: Forensic Science International: GeneticsAuthor(s): ES Chiu, K Fox, L Wolfe, S VandewoudeABSTRACTIn October 2018, Colorado Parks and Wildlife seized an animal believed to be an illegally possessed bobcat. The owner claimed the animal was a bobcat/domestic cat hybrid, exempted from license requirements. Burden of proof lay with CPW to determine the lineage of the animal. Commercial microsatellite arrays and DNA barcoding have not been developed for identification of bobcat/domestic cat hybrids, and limited time and resources prevented development of such tests for thi...
Source: Forensic Science International: Genetics - October 23, 2019 Category: Forensic Medicine Source Type: research

UrsaPlex: An STR multiplex for forensic identification of North American black bear (Ursus americanus)
Publication date: Available online 17 October 2019Source: Forensic Science International: GeneticsAuthor(s): Erin P. Meredith, Jillian K. Adkins, Jeff A. RodzenAbstractUrsaPlex is a forensic quality 5-dye multiplexed tetranucleotide STR and sex identification panel for individual genetic identification of North American black bears (Ursus americanus). The panel is validated for the identification of black bears involved in human-wildlife conflict events and poaching investigations. This is the first single multiplex panel composed solely of tetranucleotide STRs derived from black bear and bear-specific sex markers. UrsaPle...
Source: Forensic Science International: Genetics - October 19, 2019 Category: Forensic Medicine Source Type: research

Ancestry prediction efficiency of the software GenoGeographer using a z-score method and the ancestry informative markers in the Precision ID Ancestry Panel
Publication date: Available online 17 October 2019Source: Forensic Science International: GeneticsAuthor(s): Helle S. Mogensen, Torben Tvedebrink, Claus Børsting, Vania Pereira, Niels MorlingAbstractWe compared the efficiency of the freely available software GenoGeographer that includes a z-score based analysis with that of a naïve method based on the maximal likelihoods of 164 of the 165 ancestral informative markers (AIM) that are included in the commercially available kit Precision ID Ancestry Panel from Thermo Fisher Scientific. The AIM profiles were obtained by investigations with the Precision ID Ancestry Panel in ...
Source: Forensic Science International: Genetics - October 19, 2019 Category: Forensic Medicine Source Type: research

Male lineages in Brazilian populations and performance of haplogroup prediction tools
In conclusion, both haplogroup prediction tools can be useful to direct the SNP typing, but present large error rates to be used in forensic analysis, especially in predicting African haplogroups in admixed South American populations. (Source: Forensic Science International: Genetics)
Source: Forensic Science International: Genetics - October 16, 2019 Category: Forensic Medicine Source Type: research

Systematic evaluation of STRmix™ performance on degraded DNA profile data
This study examined the DNA degradation modeling capacity of STRmix™, a widely implemented DNA interpretation software program. As a part of the CAL DOJ STRmix™ v2.4 validation, a large volume of STR profile data was generated from intact template DNA exposed to DNase I for a series of increasing time intervals. The resulting degraded profile data was analyzed with STRmix™ v2.4, and the efficacy of the analysis was assessed, both in terms of how the degradation modeling parameter values from the STRmix™ analysis compared to ground truth values, and how the weight-of-evidence statistics calculated for degraded profi...
Source: Forensic Science International: Genetics - October 16, 2019 Category: Forensic Medicine Source Type: research