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in vitro Activity of Oritavancin against Planktonic and Biofilm States of Vancomycin-Susceptible and Vancomycin-Resistant Enterococci
We tested the in vitro activity of oritavancin against 60 vancomycin-susceptible enterococci (VSE) and 27 vancomycin-resistant enterococci (VRE). The oritavancin MIC ranged from ≤0.002 to 0.5μg/mL; MBBC ranged from ≤0.002 to 2μg/mL. Oritavancin has promising in vitro activity against VSE and VRE in both planktonic and biofilm states. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 15, 2018 Category: Microbiology Authors: Qun Yan, Melissa J. Karau, Robin Patel Source Type: research

Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L. (L.) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L. (Viannia) naiffi strains. The profiles of both L. (V.) shawi and L. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 12, 2018 Category: Microbiology Authors: Caroline R. Espada, Paola A. Ortiz, Jeffrey J. Shaw, Aldina M.P. Barral, Jackson M.L. Costa, Silvia R.B. Uliana, Adriano C. Coelho Source Type: research

Evaluation of a two-stage testing algorithm for the diagnosis of respiratory viral infections
New on-demand multiplex molecular respiratory viral diagnostics offer superior performance although can be expensive and some platforms cannot process multiple specimens simultaneously. We performed a retrospective study reviewing results of patients tested for respiratory viruses following introduction of a two-stage testing algorithm incorporating an initial screen with Sofia ® immunoassay then secondary Biofire Filmarray®, and compared to a period when only Filmarray® was used. Of 2976 testing episodes, 1814 underwent initial Sofia® then follow-up FilmArray®. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 12, 2018 Category: Microbiology Authors: B.J. Gardiner, C.E.V. Parker, A.R. Rabson, D.R. Snydman, S. Doron Source Type: research

Evaluation of a two-stage testing algorithm for the diagnosis of respiratory viral infections
New on-demand multiplex molecular respiratory viral diagnostics offer superior performance although can be expensive and some platforms cannot process multiple specimens simultaneously. We performed a retrospective study reviewing results of patients tested for respiratory viruses following introduction of a two-stage testing algorithm incorporating an initial screen with Sofia ® immunoassay then secondary Biofire Filmarray®, and compared to a period when only Filmarray® was used. Of 2976 testing episodes, 1814 underwent initial Sofia® then follow-up FilmArray®. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 12, 2018 Category: Microbiology Authors: BJ Gardiner, Parker CEV, AR Rabson, DR Snydman, S Doron Source Type: research

Novel class 1 integron (In1390) harboring blaGES-5 in a Morganella morganii strain recovered from a remote community
Antimicrobial resistance in Morganella morganii has been mainly acquired via plasmids and class 1 integrons. We hereby report acquisition of blaGES-5 by a M. morganii isolated in a remote community from the Amazon region. Genomic analysis revealed that blaGES-5 was harbored by a novel class 1 integron designated as In1390. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 10, 2018 Category: Microbiology Authors: Qu ézia Moura, Louise Cerdeira, Miriam R. Fernandes, Marco A. Vianello, Nilton Lincopan Tags: Short Note Source Type: research

Novel class 1 integron (In1390) harbouring blaGES-5 in a Morganella morganii strain recovered from a remote community
Antimicrobial resistance in Morganella morganii has been mainly acquired via plasmids and class 1 integrons. We hereby report acquisition of blaGES-5 by a M. morganii isolated in a remote community from the Amazon region. Genomic analysis revealed that blaGES-5 was harboured by a novel class 1 integron designated as In1390. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 10, 2018 Category: Microbiology Authors: Qu ézia Moura, Louise Cerdeira, Miriam R. Fernandes, Marco A. Vianello, Nilton Lincopan Tags: Short Note Source Type: research

In vitro combination of voriconazole with micafungin against azole-resistant clinical isolates of Aspergillus fumigatus from different geographical regions
In vitro interaction of voriconazole with micafungin was evaluated against 33 clinical Aspergillus fumigatus isolates, including azole-resistant (n=31) and -susceptible (n=2) isolates. Interaction was synergistic for only 1 resistant isolate carrying the TR34/L98H mutation. No antagonistic effects were observed for 96.8% of azole-resistant isolates. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 8, 2018 Category: Microbiology Authors: Hamed Fakhim, Afsane Vaezi, Eric Dannaoui, Cheshta Sharma, Bita Mousavi, Anuradha Chowdhary, Jacques F. Meis, Hamid Badali Tags: Note Source Type: research

In vitro combination of voriconazole with micafungin against azole-resistant clinical isolates of Aspergillus fumigatus from different geographical regions
In vitro interaction of voriconazole with micafungin was evaluated against 33 clinical Aspergillus fumigatus isolates, including azole-resistant (n=31) and –susceptible (n=2) isolates. Interaction was synergistic for only one resistant isolate carrying the TR34/ L98H mutation. No antagonistic effects were observed for 96.8% of azole-resistant isolates. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 8, 2018 Category: Microbiology Authors: Hamed Fakhim, Afsane Vaezi, Eric Dannaoui, Cheshta Sharma, Bita Mousavi, Anuradha Chowdhary, Jacques F. Meis, Hamid Badali Tags: Note Source Type: research

Differentiating clearly positive from indeterminate results: A review of irreproducible HIV-1 PCR positive samples from South Africa's Early Infant Diagnosis Program, 2010 –2015
We describe the extent of and variables associated with irreproducible HIV-1 PCR positive results within South Africa's Early Infant Diagnosis (EID) program from 2010 to 2015 and propose criteria for differentiating indeterminate from clearly positive results using the COBAS ® AmpliPrep/COBAS® TaqMan HIV-1 Qualitative Test version 2.0 (CAP/CTM Qual v2.0). Fourteen percent of specimens with an instrument-positive result that were repeat-tested yielded a negative result for which cycle threshold (Ct) proved to be the only predictive variable. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 3, 2018 Category: Microbiology Authors: Ahmad Haeri Mazanderani, Faith Moyo, Tendesayi Kufa, Jean Maritz, Gayle G. Sherman Source Type: research

Automatic notification and infectious diseases consultation for patients with Staphylococcus aureus bacteremia
Observational studies suggest that Infectious Diseases (ID) consultation is associated with improved outcomes for patients with Staphylococcus aureus bacteremia (SAB), but few studies have sought to standardize timely ID consultation through automatic notification by the Microbiology laboratory. In this 3-year quasi-experimental evaluation, introduction of this change resulted in increased ID consultation for SAB (70% versus 100%, P   (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 3, 2018 Category: Microbiology Authors: Lucas Djelic, Nisha Andany, Jeffrey Craig, Nick Daneman, Andrew Simor, Jerome A. Leis Tags: Note Source Type: research

Differentiating Clearly Positive from Indeterminate Results: A Review of Irreproducible HIV-1 PCR Positive Samples from South Africa's Early Infant Diagnosis Program, 2010 –2015
We describe the extent of and variables associated with irreproducible HIV-1 PCR positive results within South Africa's Early Infant Diagnosis (EID) program from 2010 to 2015 and propose criteria for differentiating indeterminate from clearly positive results using the COBAS ® AmpliPrep/COBAS® TaqMan HIV-1 Qualitative Test version 2.0 (CAP/CTM Qual v2.0). Fourteen percent of specimens with an instrument-positive result that were repeat tested yielded a negative result for which cycle threshold (Ct) proved to be the only predictive variable. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 3, 2018 Category: Microbiology Authors: Ahmad Haeri Mazanderani, Faith Moyo, Tendesayi Kufa, Jean Maritz, Gayle G Sherman Source Type: research

Automatic Notification and Infectious Diseases Consultation for Patients with Staphylococcus aureus Bacteremia
Observational studies suggest that Infectious Diseases (ID) consultation is associated with improved outcomes for patients with Staphylococcus aureus bacteremia (SAB) but few studies have sought to standardize timely ID consultation through automatic notification by the Microbiology laboratory. In this 3-year quasi-experimental evaluation, introduction of this change resulted in increased ID consultation for SAB (70% vs. 100%, p (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - March 3, 2018 Category: Microbiology Authors: Lucas Djelic, Nisha Andany, Jeffrey Craig, Nick Daneman, Andrew Simor, Jerome A. Leis Tags: Note Source Type: research

Lack of effect of antibiotics on biopsy culture results in vertebral osteomyelitis
We report a retrospective study of 173 patients with vertebral osteomyelitis evaluating the effect of antibiotics administered prior to percutaneous and open biopsy cultures. Antibiotics given prior to these biopsies did not have a significant effect on the yield of cultures. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 26, 2018 Category: Microbiology Authors: Louis D. Saravolatz, Vladimir Labalo, Joel Fishbain, Susan Szpunar, Leonard B. Johnson Tags: Note Source Type: research