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Activity of CD101, a long-acting echinocandin, against clinical isolates of Candida auris
CD101 is a new echinocandin with a prolonged half-life. CD101 was tested by broth microdilution against 100 isolates of the emerging yeast Candida auris. It showed activity against most isolates, including some that were resistant to other echinocandins. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 7, 2017 Category: Microbiology Authors: Elizabeth L. Berkow, Shawn R. Lockhart Tags: Note Source Type: research

NDM-1 encoded by a pNDM-HN380-Like Plasmid pNDM-BJ03 in clinical Enterobacter cloacae
A carbapenemase-producing Enterobacter cloacae hhy03 with a blaNDM-1 and blaSHV-12-coharboring plasmid was isolated from a sputum specimen of a patient. This is the third nucleotide sequence report of blaNDM-1-harboring plasmid from Enterobacter cloacae that have caused lethal infections in China, indicationg the spread of NDM-1 by IncX3 plasmid between Enterobacteriaceae. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 7, 2017 Category: Microbiology Authors: Yang L ü, Wei Liu, Hui Liang, Shulong Zhao, Wei Zhang, Jia Liu, Cheng Jin, Hongyan Hu Source Type: research

Determination of Tedizolid Susceptibility Interpretive Criteria for Gram-Positive Pathogens According to Clinical and Laboratory Standards Institute Guidelines
This article summarizes the key components used to establish the Clinical Laboratory Standards Institute (CLSI) breakpoints for tedizolid. First, in vitro studies using recent surveillance and clinical trial isolates ascertained minimal inhibitory concentration (MIC) distributions against pertinent organisms, including staphylococci, streptococci, and enterococci. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 7, 2017 Category: Microbiology Authors: Mekki Bensaci, Shawn Flanagan, Taylor Sandison Source Type: research

Comparison of Abbott Architect ®, Siemens Immulite®, and Diasorin Liaison® for determination of Epstein–Barr virus serological diagnosis
This study compared the performance of 3 automated immunoassays, Architect ® (Abbott), Immulite® (Siemens) and Liaison® (Diasorin), for Epstein–Barr virus (EBV) serology. Ninety-one serum samples collected in Amiens University Hospital were analyzed for the presence of Viral Capsid Antigen (VCA) IgG and IgM and Epstein–Barr Nuclear Antigen (EBNA) IgG. The agreement between the 3 assays was calculated for each marker individually and for determination of the EBV profile, based on interpretation of the combination of these 3 EBV markers. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 6, 2017 Category: Microbiology Authors: Catherine Fran çois, Christine Segard, Maryline Bouvier, Martine Stefanski, Christine Pannier, Patricia Zawadzki, Catherine Roussel, Denise Hecquet, Gilles Duverlie, Etienne Brochot, Sandrine Castelain Source Type: research

Occurrence and improvement of renal dysfunction and serum potassium abnormality during administration of liposomal amphotericin B in patients with haematological disorders: A retrospective analysis
Liposomal amphotericin B (L-AMB) has the potential to cause two major adverse events, renal dysfunction and serum potassium abnormality; however, appropriate clinical management of these events remains unclear. We retrospectively analyzed data regarding 128 haematology patients who received L-AMB in our institute and examined the association between clinical characteristics and renal dysfunction or serum potassium abnormality. We found that the median weight-normalized dose of L-AMB was 2.69mg/kg and the median administration period was 16days. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 6, 2017 Category: Microbiology Authors: Hiroyuki Yamazaki, Tadakazu Kondo, Kazunai Aoki, Kouhei Yamashita, Akifumi Takaori-Kondo Source Type: research

Klebsiella pneumoniae co-producing KPC and RmtG, finally targeting Switzerland
A carbapenem- and pan-aminoglycoside-resistant Klebsiella pneumoniae strain was isolated from a Brazilian patient hospitalized in a Swiss hospital. The clinical isolate carried genes encoding the KPC-2 carbapenemase and the RmtG 16S rRNA methyltransferase. This is the first report of a carbapenem-resistant K. pneumoniae producing RmtG in Europe. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 6, 2017 Category: Microbiology Authors: Stefano Mancini, Laurent Poirel, Myriam Corthesy, Gilbert Greub, Patrice Nordmann Tags: Note Source Type: research

Comparison of Abbott Architect ®, Siemens Immulite® and Diasorin Liaison® for determination of Epstein–Barr virus serological diagnosis
This study compared the performance of three automated immunoassays, Architect ® (Abbott), Immulite® (Siemens) and Liaison® (Diasorin), for Epstein–Barr virus (EBV) serology. Ninety-one serum samples collected in Amiens University Hospital were analyzed for the presence of Viral Capsid Antigen (VCA) IgG and IgM and Epstein–Barr Nuclear Antigen (EBNA) IgG. The agreement between the three assays was calculated for each marker individually and for determination of the EBV profile, based on interpretation of the combination of these three EBV markers. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 6, 2017 Category: Microbiology Authors: Catherine Fran çois, Christine Segard, Maryline Bouvier, Martine Stefanski, Christine Pannier, Patricia Zawadzki, Catherine Roussel, Denise Hecquet, Gilles Duverlie, Etienne Brochot, Sandrine Castelain Source Type: research

Synovial fluid multiplex PCR is superior to culture for detection of low-virulent pathogens causing periprosthetic joint infection
Analysis of joint aspirate is the standard preoperative investigation for diagnosis of periprosthetic joint infection (PJI). We compared the diagnostic performance of culture and multiplex polymerase chain reaction (PCR) of synovial fluid for diagnosis of PJI. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 2, 2017 Category: Microbiology Authors: Christian Morgenstern, Sabrina Cabric, Carsten Perka, Andrej Trampuz, Nora Renz Source Type: research

Failure of daptomycin β-lactam combination therapy to prevent resistance emergence in Enterococcus faecium
We report failure of this strategy and the importance of source control. Mutations were detected in the LiaF and cls genes in DNS isolates. A single DNS isolate cont ained an unrecognised mutation, which requires confirmation. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 2, 2017 Category: Microbiology Authors: Vidthiya Menon, Rebecca Davis, Nick Shackel, Bjorn A. Espedido, Alicia G. Beukers, Slade O. Jensen, Sebastiaan J. van Hal Tags: Note Source Type: research

Central Nervous System Blastomycosis Diagnosed using the MVista ® Blastomyces Quantitative Antigen Enzyme Immunoassay Test on Cerebrospinal Fluid: A Case Report and Review of the Literature
Blastomyces dermatitidis is a thermally dimorphic fungus that is capable of causing pulmonary and extra-pulmonary disease, including infections of the central nervous system (CNS). Diagnosis of CNS blastomycosis with non-invasive testing can be difficult, and a surgical biopsy may ultimately be required for microbiological and/or histopathological confirmation. A case of B. dermatitidis meningitis is presented where the diagnosis was made by testing cerebrospinal fluid (CSF) using the MVista ® Blastomyces Quantitative Antigen Enzyme Immunoassay test. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - October 30, 2017 Category: Microbiology Authors: Andrew Walkty, Yoav Keynan, James Karlowsky, Perry Dhaliwal, John Embil Source Type: research

rpoB gene sequencing highlights the prevalence of an E. miricola cluster over other Elizabethkingia species among UK Cystic Fibrosis patients
Difficulties in distinguishing species of the Elizabethkingia genus by MALDI-TOF prompted use of rpoB sequencing to investigate species distribution among 44 isolates from cystic fibrosis (CF) patients. Forty-three isolates from 38 patients formed a cluster comprising E. miricola and proposed novel species E. bruuniana sp. nov., the exception clustering with proposed species E. ursingii sp. nov., also part of this wider cluster. All 44 isolates were PCR-positive for urease gene ureG, whereas only one of 23 E. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - October 26, 2017 Category: Microbiology Authors: Dervla T.D. Kenna, Alice Fuller, Kate Martin, Claire Perry, Rachel Pike, Phillipa J. Burns, Omendra Narayan, Stuart Wilkinson, Robert Hill, Neil Woodford, Julie M.J. Logan, Jane F. Turton Source Type: research

Comparison of the Cepheid GeneXpert CT/NG assay to the Hologic Aptima Combo2 assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in self-collected rectal swabs
Self-collected rectal-swabs were tested for CT and NG on GeneXpert CT/NG as compared to APTIMA Combo2. Of 448 rectal-swabs, 22 were positive for CT; 7 for NG on both assays; two were discordant. Sensitivity and specificity of GeneXpert was 95.5% and 99.7% for chlamydia, respectively; for gonorrhea both were 100%. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - October 25, 2017 Category: Microbiology Authors: Laura Dize, Barbara Silver, Charlotte Gaydos Tags: Note Source Type: research

Comparison of the Cepheid GeneXpert CT/NG Assay to the Hologic Aptima Combo 2 Assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in self-collected rectal swabs
Self-collected rectal-swabs were tested for CT and NG on GeneXpert CT/NG as compared to APTIMA Combo2. Of 448 rectal-swabs, 22 were positive for CT; 7 for NG on both assays; two were discordant. Sensitivity and specificity of GeneXpert was 95.5% and 99.7% for chlamydia, respectively; for gonorrhea both were 100%. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - October 25, 2017 Category: Microbiology Authors: Laura Dize, Barbara Silver, Charlotte Gaydos Tags: Note Source Type: research

Chromogenic detection procedure for the multidrug-resistant, neonatal sepsis-associated clone Staphylococcus capitis NRCS-A
The multiresistant Staphylococcus capitis clone NRCS-A is a major pathogen in neonates worldwide. We show that NRCS-A grows as mauve colonies with a cream-color halo after a 5-days incubation on MRSA Brilliance 2 agar (Oxoid ®). This innovative protocol will ease the screening of clinical and environmental niches of this clone. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - October 23, 2017 Category: Microbiology Authors: Marine Butin, Yann Dumont, Jean-Philippe Rasigade, Patricia Martins Simoes, Louise Hoden, Jean-Charles Picaud, Fr édéric Laurent Tags: Note Source Type: research

Comparative study of 3 carbapenem-hydrolysis methods for the confirmation of carbapenemase production in Enterobacteriaceae
The goals of the study were to examine the analytic and performance parameters of 2 commercial rapid carbapenem-hydrolysis assays, the β-CARBA test (Bio-Rad) and the Rapid CARB Blue Kit (ROSCO) in comparison with an in-house CARBA NP assay for the detection of Carbapenemase-Producing Enterobacteriaceae (CPE). Their performance was evaluated following growth on 2 chromogenic CPE screening media. The sensitivity was highest (91%) in the β-CARBA test when used from the mSuperCARBA™ plates and was lowest when the same test was used from the chromID™ CARBA plates (75%). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - October 19, 2017 Category: Microbiology Authors: Ohad Shalom, Amos Adler Source Type: research