Diagnostic Microbiology and Infectious Disease This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Proteus mirabilis harbouring Carbapenemase NDM-5 and ESBL VEB-6 detected in Austria
We describe a case of carbapenemase-harbouring Proteus mirabilis together with detection of NDM-5 in Austria accompanied by other bacterial strains with a wide range of beta-lactamases including OXA-181 and VEB-6. Isolates were obtained from a subphrenic abscess from one patient who was previously treated with broad-spectrum antibiotics in Bangladesh. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Thomas Valentin, Gebhard Feierl, Lilian Masoud-Landgraf, Peter Kohek, Josefa Luxner, Gernot Zarfel Tags: Note Source Type: research

Emergence of multidrug-resistant clones in levofloxacin non-susceptible Streptococcus pneumoniae isolates in Korea
The use of fluoroquinolones to treat respiratory tract infections and pneumonia due to Streptococcus pneumoniae has affected the emergence of resistance to this class of drugs. Increasing pneumococcal resistance to levofloxacin has become a major public health concern. We investigated the prevalence and genetic characteristics of levofloxacin non-susceptible S. pneumoniae (LNSP) clinical isolates in Korea. A total of 43 LNSP isolates collected from a national surveillance study at 13 tertiary hospitals between 2008 and 2014 were analyzed for serotype and antimicrobial susceptibilities to 19 antimicrobial agents as well as ...
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Jin Yang Baek, Cheol-In Kang, So Hyun Kim, Kwan Soo Ko, Doo Ryeon Chung, Kyong Ran Peck, Nam Yong Lee, Jae-Hoon Song Source Type: research

Initial Performance Evaluation of a Spotted Array Mobile Analysis Platform (MAP) for the Detection of Influenza A/B, RSV and MERS Coronavirus
Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical RT-PCR assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for RSV, and 80% (4/5) for negative specimens. The approximate LOD of the MAP was 30 copies /assay for RSV and 1500 copies/assay for MERS Coronavirus. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Justin Hardick, David Metzgar, Lisa Risen, Christopher Myers, Melinda Balansay, Trent Malcom, Richard Rothman, Charlotte Gaydos Tags: Note Source Type: research

Evaluation of the Carbapenem Detection Set ™ for the detection and characterization of carbapenemase-producing Enterobacteriaceae
The objective of this study was to assess the performance of the Carbapenemase detection set ™ (CDS; Mast Diagnostics) in association with (i) the EUCAST meropenem screening cut-off and (ii) the faropenem-temocillin algorithm (FTa) for the screening of carbapenemase-producing Enterobacteriaceae (CPE). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Laurent Dortet, Sandrine Bernabeu, Camille Gonzalez, Thierry Naas Source Type: research

Evaluation of Sepsis Flow Chip for identification of Gram-negative bacilli and detection of antimicrobial resistance genes directly from positive blood cultures
In this study, the new Sepsis Flow Chip (SFC) assay for identification of Gram-negative bacteria and their antimicrobial resistance genes was evaluated in positive blood cultures (BCs). SFC is a microarray with a broad panel comprising the most frequent causative agents of sepsis and antimicrobial resistance genes associated with them. A total of 100 prospective BCs, positive for Gram-negative bacilli, were assessed in the routine of the clinical microbiology laboratory and also applying the SFC assay. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 15, 2018 Category: Microbiology Authors: Carlos Rodr íguez-Lucas, M. Rosario Rodicio, Isabel Costales, José Antonio Boga, Fernando Vazquez, Javier Fernández Source Type: research

Evaluation of Sepsis Flow Chip for identification of Gram-negative bacilli and detection of antimicrobial resistance genes directly from positive blood cultures
In this study, the new Sepsis Flow Chip (SFC) assay for identification of Gram-negative ) bacteria and their antimicrobial resistance genes was evaluated in positive blood cultures (BCs). SFC is a microarray with a broad panel comprising the most frequent causative agents of sepsis and antimicrobial resistance genes associated with them. A total of 100 prospective BCs, positive for Gram-negative bacilli (GNB), were assessed in the routine of the clinical microbiology laboratory and also applying the SFC assay. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 15, 2018 Category: Microbiology Authors: Carlos Rodr íguez-Lucas, M. Rosario Rodicio, Isabel Costales, José Antonio Boga, Fernando Vazquez, Javier Fernández Source Type: research

Value of blood culture time to positivity in identifying complicated nontyphoidal Salmonella bacteremia
Few studies analyzed the association between blood culture time to positivity (TTP) and risk of complicated nontyphoidal Salmonella (NTS) bacteremia. We conducted a retrospective study of 206 patients (aged 60.4 ± 17.4 years) with NTS bacteremia during a 30-month period. Complicated NTS bacteremia was defined as the presence of 30-day mortality, complicated infection requiring surgery or abscess drainage, or requirement of intensive care unit admission. Serogroup D (75.7%) was the predominant isolates. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 13, 2018 Category: Microbiology Authors: Shang-Yu Chen, Tzu-Hua Weng, Wen-Pin Tseng, Chia-Ming Fu, Hui-Wen Lin, Chun-Hsing Liao, Tai-Fen Lee, Po-Ren Hsueh, Cheng-Chung Fang, Shey-Ying Chen Source Type: research

Diagnostic aptitude of West Nile virus-like particles expressed in insect cells
West Nile virus is a globally spread zoonotic arbovirus. The laboratory diagnosis of WNV infection relies on virus identification by RT-PCR or on specific antibody detection by serological tests, such as ELISA or virus-neutralization. These methods usually require a preparation of the whole virus as antigen, entailing biosafety issues and therefore requiring BSL-3 facilities. For this reason, recombinant antigenic structures enabling effective antibody recognition comparable to that of the native virions, would be advantageous as diagnostic reagents. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 10, 2018 Category: Microbiology Authors: Bel én Rebollo, Javier Sarraseca, Mª José Rodríguez, Antonio Sanz, Miguel Ángel Jiménez-Clavero, Ángel Venteo Source Type: research

Diagnostic aptitude of west nile virus-like particles expressed in insect cells
West Nile virus is a globally spread zoonotic arbovirus. The laboratory diagnosis of WNV infection relies on virus identification by RT-PCR or on specific antibody detection by serological tests, such as ELISA or virus-neutralization. These methods usually require a preparation of the whole virus as antigen, entailing biosafety issues and therefore requiring BSL-3 facilities. For this reason, recombinant antigenic structures enabling effective antibody recognition comparable to that of the native virions, would be advantageous as diagnostic reagents. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 10, 2018 Category: Microbiology Authors: Bel én Rebollo, Javier Sarraseca, Mª José Rodríguez, Antonio Sanz, Miguel Ángel Jiménez-Clavero, Ángel Venteo Source Type: research

Evaluation of the CRE and ESBL ELITe MGB ® kits for the accurate detection of carbapenemase- or CTX-M-producing bacteria
As carbapenemase-producing Enterobacteriaceae (CPE) and extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. The ELITe InGenius is a fully automated sample-to-result system designed for the extraction and detection by multiplex real-time PCR of carbape nemases KPC, NDM, VIM, IMP, and OXA-48-like variants and CTX-M group 1 and 9-producers from diverse sample matrices such as colonies, positive blood cultures and rectal swabs. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 9, 2018 Category: Microbiology Authors: Delphine Girlich, Sandrine Bernabeu, Nicolas Fortineau, Laurent Dortet, Thierry Naas Source Type: research

Evaluation of a real-time PCR assay for detection of Mycoplasma genitalium and macrolide resistance-mediating mutations from clinical specimens
Mycoplasma genitalium (MG) is a sexually transmitted pathogen for which there is no FDA-approved diagnostic test available in the United States. A modified real-time polymerase chain reaction assay for detecting MG and simultaneously identifying macrolide resistance mutations from clinical specimens was evaluated and proved to be sensitive and accurate for diagnostic purposes. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 9, 2018 Category: Microbiology Authors: Li Xiao, Ken B. Waites, Hong Wang, Barbara Van Der Pol, Amy E. Ratliff, William M. Geisler Tags: Note Source Type: research

Evaluation of the CRE and ESBL ELITe MGB ® kits for the accurate detection of carbapenemase- or CTX-M-producing bacteria
As carbapenemase-producing Enterobacteriaceae (CPE) and extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. The ELITe InGenius is a fully automated sample-to-result system designed for the extraction and detection by multiplex real-time PCR of carbape nemases KPC, NDM, VIM, IMP, and OXA-48-like variants and CTX-M group 1 and 9-producers from diverse sample matrices such as colonies, positive blood cultures and rectal swabs. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 9, 2018 Category: Microbiology Authors: Delphine Girlich, Sandrine Bernabeu, Nicolas Fortineau, Laurent Dortet, Thierry Naas Source Type: research

Evaluation of a real-time PCR assay for detection of Mycoplasma genitalium and macrolide resistance-mediating mutations from clinical specimens
Mycoplasma genitalium (MG) is a sexually transmitted pathogen for which there is no FDA approved diagnostic test available in the U.S. A modified real-time PCR assay for detecting MG and simultaneously identifying macrolide resistance mutations from clinical specimens was evaluated and proved to be sensitive and accurate for diagnostic purposes. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 9, 2018 Category: Microbiology Authors: Li Xiao, Ken B. Waites, Hong Wang, Barbara Van Der Pol, Amy E. Ratliff, William M. Geisler Tags: Note Source Type: research

PCR ribotyping and antimicrobial susceptibility testing of isolates of Clostridium difficile cultured from toxin-positive diarrheal stools of patients receiving medical care in Canadian hospitals: the Canadian Clostridium difficile Surveillance Study (CAN-DIFF) 2013 –2015
Clostridium difficile toxin-positive diarrheal stool specimens submitted to eight Canadian hospital laboratories from 2013 to 2015 were cultured. Polymerase chain reaction ribotyping of isolates was performed using an internationally standardized, high-resolution capillary gel-based electrophoresis protocol and antimicrobial susceptibility testing conducted by CLSI-defined agar dilution (M11-A8, 2012). Among the 1310 isolates of C. difficile cultured, 141 different ribotypes were identified; the most common ribotypes were 027 (24.5% of isolates), 014 (7.7%), 020 (6.6%), 106 (6.1%), and 002 (4.6%). (Source: Diagnostic Micro...
Source: Diagnostic Microbiology and Infectious Disease - February 4, 2018 Category: Microbiology Authors: James A. Karlowsky, Heather J. Adam, Tyler Kosowan, Melanie R. Baxter, Kim A. Nichol, Nancy M. Laing, George Golding, George G. Zhanel Source Type: research