Immune Cell and Cell Cluster Phenotyping, Quantitation, and Visualization Using In Silico Multiplexed Images and Tissue Cytometry
In this report, we describe and give an example of a workflow to phenotype, quantitate, and visualize greater than four biomarkers in silico utilizing multiplexed fluorescence histology and the TissueFAXS quantitative imaging system with a conventional microscopy setup. Biomarkers were conjugated to Cy3 or Cy5. Multiplexed staining was performed on formalin ‐fixed paraffin‐embedded tissue sections. We imaged the slides, inactivated the dyes, and repeated the process until all biomarkers were stained. Phenotype profiles were built based on in silico combinations of the biomarkers. We used algorithms that aligned all ima...
Source: Cytometry Part A - November 23, 2018 Category: Molecular Biology Authors: Kim R.M. Blenman,
Marcus W. Bosenberg Tags: Technical Note Source Type: research
A Flow Cytometric Study of ER Stress and Autophagy
AbstractThe mechanistic link between ER stress, autophagy, and resultant cell death was investigated by the use of drugs Thapsigargin (Tg) and Chloroquine (CQ) with prior induction and or blockade of autophagy and apoptosis which modulated the ER stress response and resultant form of cell death. All these biological processes can be measured flow cytometrically allowing the determination of the type of cell death, G1 cell cycle arrest, cell cycle dependent measurement of ER stress transducer PERK, misfolded proteins, reticulophagy, and autophagy marker LC3B. Jurkat cells after Tg or CQ treatment became necrotic and apoptot...
Source: Cytometry Part A - November 19, 2018 Category: Molecular Biology Authors: A Popat,
AA Patel,
G Warnes Tags: Original Article Source Type: research
Fast and Quantitative Evaluation of Human Leukocyte Interaction with Aspergillus fumigatus Conidia by Flow Cytometry
AbstractSystemic infections with the opportunistic moldAspergillus fumigatus are a great threat to immunocompromised patients such as transplant recipients. Immunological research onA. fumigatus involves the measurement of phagocytosis of fungal conidia (spores) by human phagocytes. Here, we present a fast and flexible way to analyze phagocytosis by flow cytometry using fluorescein isothiocyanate (FITC) labeling of conidia prior to co ‐incubation with human leukocytes and an anti‐FITC counterstaining step postincubation to allow the discrimination of internalized and adherent conidia. In contrast to many other protocol...
Source: Cytometry Part A - November 19, 2018 Category: Molecular Biology Authors: Susann Hartung,
Christopher Rauh,
Thi Ngoc Mai Hoang,
Susanne Jahreis,
Kathleen Wagner,
Juliane Macheleidt,
Axel A. Brakhage,
Silke Rummler,
Andreas Hochhaus,
Marie Lilienfeld ‐Toal Tags: Technical Note Source Type: research
Cytometry part A instructions to contributors
Cytometry Part A, Volume 93, Issue 11, Page 1172-1172, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Issue Information ‐Instructions to Contributors Source Type: research
ISAC member application
Cytometry Part A, Volume 93, Issue 11, Page 1166-1167, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Issue Information ‐ISAC Member Application Source Type: research
Editorial Policy
Cytometry Part A, Volume 93, Issue 11, Page 1165-1165, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Issue Information ‐Editorial Policy Source Type: research
Journal roundup
Cytometry Part A, Volume 93, Issue 11, Page 1083-1083, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Features Source Type: research
In this issue
Cytometry Part A, Volume 93, Issue 11, Page 1082-1082, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Features Source Type: research
Volume 93A, Number 11, November 2018 Cover Image
AbstractLabel ‐free cytometry of Cell motility. The authors evaluated the application of digital holographic cytometry for assessing the motility of cancer cells in comparison to more established approaches. They investigated the motility of human melanoma cells lines with known relative motility and metastatic potential in different experimental set‐ups. See Zhang and Judson in this issue.Cover design by B ärbel Beran [www.beran-design.de]. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Volume 93A, Number 11, November 2018 Cover Image Source Type: research
Issue Information – Editorial board
Cytometry Part A, Volume 93, Issue 11, Page 1073-1073, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Issue Information – Editorial board Source Type: research
Issue Information – Publication schedule
Cytometry Part A, Volume 93, Issue 11, Page 1079-1079, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Issue Information – Publication schedule Source Type: research
Volume 93A, Number 11, November 2018 Table of Contents
Cytometry Part A, Volume 93, Issue 11, Page 1076-1078, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Tags: Online TOC Source Type: research
Cytometry in the air
Cytometry Part A, Volume 93, Issue 11, Page 1085-1086, November 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - November 16, 2018 Category: Molecular Biology Authors: Attila T árnok Tags: Editorial Source Type: research
VyCAP's puncher technology for single cell identification, isolation, and analysis
AbstractHere we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The technology is compatible with commercially available downstream workflows and instrumentation. Here we focus on the isolation of CTC but the Puncher technology can be applied to isolate single cells from liquid biopsies and more general from cell suspensions. It is especially suited for cell suspensions that contain:
Cells of interest at a frequency of 1 per 10,000 or les...
Source: Cytometry Part A - November 14, 2018 Category: Molecular Biology Authors: Michiel Stevens,
Lisa Oomens,
Joska Broekmaat,
Joris Weersink,
Fikri Abali,
Joost Swennenhuis,
Arjan Tibbe Tags: CTC Technologies and Tools Source Type: research
Deep ultraviolet lasers for flow cytometry
In this study, we evaluated a newly available laser emitting at 280 nm as a possible laser source for exciting these dyes. Since deep UV polymer dyes are not yet avail able, we used quantum nanoparticles (Qdots) as a surrogate probe to assess the utility of this laser wavelength for flow cytometry. Deep UV laser light was found to excite Qdots as well as traditional UV sources. Deep UV 280 nm did not excite BUV dyes well, suggesting that BUV and deep UV polymers w ill be spectrally compatible with low crossbeam spillover issues. Deep UV excitation did excite considerable autofluorescence in the violet to blue range, a limi...
Source: Cytometry Part A - November 13, 2018 Category: Molecular Biology Authors: William Telford,
Thierry Georges,
Clint Miller,
Pascal Voluer Tags: Technical Note Source Type: research
