A facile one-pot method to prepare peroxidase-like nanogel artificial enzymes for highly efficient and controllable catalysis
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Hejin Shi, Yong Liu, Rui Qu, Yuanfeng Li, Rujiang Ma, Yingli An, Linqi ShiAbstractNovel artificial enzymes are highly desired to overcome the shortcomings of natural enzymes during industrial or biological applications. Here we designed and prepared nanogel-based artificial enzymes (NAEs) to mimic natural horseradish peroxidase (HRP) using a facile one-pot, scalable method. The poly(N-isopropylacrylamide) (PNIPAM) matrix provided a temperature-responsive and size-controllable scaffold for the NAEs, and 1-vinylimidazole (Vi...
Source: Colloids and Surfaces B: Biointerfaces - November 23, 2018 Category: Biochemistry Source Type: research

Immobilization of functional polymers on poly(4-benzoyl-pxylylene-co-p-xylylene) films via photochemical conjugation for modulation of cell adhesion
In this study, poly(ethylene glycol) (PEG) was conjugated onto Benzoyl-PPX via UV illumination. The deposited PEG films could effectively reduce protein adsorption and cell attachment. The low-fouling properties of the PEG films were positively correlated with the molecular weight and concentration of PEG. We found that a PEG film, thicker than 16 nm and with a water contact angle of 30°, is a prerequisite for effective inhibition of cell attachment. We also demonstrated that the PEG coating was stable under acidic and basic environments. Furthermore, poly(N-isopropyl acrylamide), PNIPAAm, could be also tethered...
Source: Colloids and Surfaces B: Biointerfaces - November 23, 2018 Category: Biochemistry Source Type: research

Near-infrared fluorescent carbon dots encapsulated liposomes as multifunctional nano-carrier and tracer of the anticancer agent cinobufagin in vivo and in vitro
In this study, the liposomes loaded with hydrophilic near-infrared carbon dots as a nano-carrier and tracer of lipophilic anticancer agent cinobufagin were developed. Prepared liposomes were characterized by particle size, morphology and entrapment efficiency. The drug release behavior, the tracer function, the anticancer effect and the side effect were investigated in vitro and in vivo. It was observed that the photoluminescence emission of carbon dots could be strongly enhanced up to 5 times by nano-liposomes. Due to this property, the bio-imaging of CDs + CB liposomes in vitro and in vivo could be clearly ...
Source: Colloids and Surfaces B: Biointerfaces - November 23, 2018 Category: Biochemistry Source Type: research

Characterization of neurocalcin delta membrane binding by biophysical methods
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Emmanuelle Hoareau, Nicolas Belley, Kristina Klinker, Bernard Desbat, Élodie BoisselierAbstractNeurocalcin delta (NCALD) is a member of the neuronal calcium sensors protein family. In the retina, NCALD is expressed by ganglion and amacrine cells. NCALD is composed of 4 EF-hand motifs but only 3 of them may bind calcium. The binding of calcium induces a conformational change of the protein which leads to the extrusion of its N-terminal myristoyl group as well as some hydrophilic residues. This mechanism, named calciu...
Source: Colloids and Surfaces B: Biointerfaces - November 22, 2018 Category: Biochemistry Source Type: research

Si-doping increases the adjuvant activity of hydroxyapatite nanorods
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Xiupeng Wang, Shu Ihara, Xia Li, Atsuo Ito, Yu Sogo, Yohei Watanabe, Noriko M. Tsuji, Atsushi YamazakiAbstractRecombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET ...
Source: Colloids and Surfaces B: Biointerfaces - November 22, 2018 Category: Biochemistry Source Type: research

Paclitaxel-betulinic acid hybrid nanosuspensions for enhanced anti-breast cancer activity
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Ruoning Wang, Mengmeng Yang, Guoyuan Li, Xin Wang, Zhenhai Zhang, Hongzhi Qiao, Jun Chen, Zhipeng Chen, Xiaobin Cui, Junsong LiAbstractPaclitaxel-betulinic acid hybrid nanosuspensions (PTX-BA-NP) with increased anti-breast cancer activity were developed. The resultant nanosuspensions (NP) had a mean particle size of 282.54 ± 5.4 nm, a polydispersity index of approximately 0.242 ± 0.02, a zeta potential of -19.7 ± 0.19 mV and a redispersibility ind...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

A new catechol-functionalized polyamidoamine as an effective SPION stabilizer
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Marco Galli, Beatrice Rossotti, Paolo Arosio, Anna Maria Ferretti, Monica Panigati, Elisabetta Ranucci, Paolo Ferruti, Anna Salvati, Daniela MaggioniAbstractA synthetic strategy was established for decorating and stabilizing superparamagnetic iron oxide nanoparticles (SPIONs) with a zwitterionic linear polyamidoamine (PAA). The strategy was successfully tested with a PAA coded ISA23 previously found endowed with interesting biological properties, such as biocompatibility, degradability in aqueous media and stealth-like pro...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

A novel pharmacological approach of herbal mediated cerium oxide and silver nanoparticles with improved biomedical activity in comparison with Lawsonia inermis
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Shanker Kalakotla, Naradala Jayarambabu, G. Krishna Mohan, Rabiatul Basria S.M.N. Mydin, V. Ramamohan GuptaAbstractDiabetes mellitus is one of the threatening, non-communicable and chronic ailments worldwide since ancient times to the current stage of human existence. The utilization of nanoparticles as a medicine in the treatment of diabetes is an attractive proposition. In the present study, herbal mediated cerium oxide nanoparticles (HMCeO2 NPs), herbal mediated silver nanoparticles (HMAg NPs) and Lawsonia intermix extr...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Influences of tantalum pentoxide and surface coarsening on surface roughness, hydrophilicity, surface energy, protein adsorption and cell responses to PEEK based biocomposite
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Shiqi Mei, Lili Yang, Yongkang Pan, Deqiang Wang, Xuehong Wang, Tingting Tang, Jie WeiAbstractPolyetheretherketone (PEEK) biomaterial has become increasingly popular in orthopedic applications due to its favorable biocompatibility, biostability, mechanical strength and elastic modulus similar to natural bones. In this research, in order to improve the biological performances of PEEK, tantalum pentoxide (Ta2O5) was incorporated into PEEK to fabricate PEEK/Ta2O5 composites (PTC) using a method of cold press-sintering, and su...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Mai Bay Stie, Henrik Seir Thoke, Olaf-Georg Issinger, Jennifer Hochscherf, Barbara Guerra, Lars F. OlsenAbstractWe have successfully encapsulated two proteins, bovine serum albumin (BSA) and p53, in chitosan-tripolyphosphate (TPP) nanoparticles at various pH values from 5.5 to 6.5 and delivered the particles to human melanoma cells. The particles have diameters ranging from 180 nm to 280 nm and a zeta potential of +15 to + 40 mV. Cellular uptake of the particles by human skin melanoma cel...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Antibiofilm effect of chlorhexidine-carrier nanosystem based on iron oxide magnetic nanoparticles and chitosan
This study synthesized and characterized a chlorhexidine (CHX)-carrier nanosystem based on iron oxide magnetic nanoparticles (IONPs) and chitosan (CS), and evaluated its antimicrobial effect on mono- and dual-species biofilms of Candida albicans and Streptococcus mutans. CHX was directly solubilized in CS-coated IONPs and maintained under magnetic stirring for obtaining the IONPs-CS-CHX nanosystem. Antimicrobial susceptibility testing for planktonic cells was performed by determining the minimum inhibitory concentration (MIC) of the nanosystem and controls. The effects of the IONPs-CS-CHX nanosystem on the formation of mon...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

pH Responsive 5-Fluorouracil Loaded Biocompatible Nanogels For Topical Chemotherapy of Aggressive Melanoma
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Prashant Sahu, Sushil K. Kashaw, Samaresh Sau, Varun Kushwah, Sanyog Jain, Ram K. Agrawal, Arun K. IyerAbstractCombating melanoma via topical route is a highly challenging task due to low selectivity, poor efficacy and impeding biological environment of the skin. In the present study, we engineered a chitosan based pH responsive biodegradable nanogel (FCNGL), encapsulated with 5-FU that was effective even at very low drug doses (0.2% w/v) against melanoma. The FCNGL was synthesized by ion gelation technique exhibited nano-...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Comprehensive measurements of salivary pellicle thickness formed at different intraoral sites on Si wafers and bovine enamel
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Sabine Güth-Thiel, Ines Kraus-Kuleszka, Hubert Mantz, Wiebke Hoth-Hannig, Hendrik Hähl, Johanna Dudek, Karin Jacobs, Matthias HannigAbstractThe salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the ...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Multi-functional core-shell Fe3O4@Au nanoparticles for cancer diagnosis and therapy
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Rajkumar S., Prabaharan M.AbstractMulti-functional core-shell Fe3O4@Au nanoparticles (Fe3O4@Au-DOX-mPEG/PEG-FA NPs) conjugated with doxorubicin (DOX), methoxy poly(ethylene glycol) (mPEG), and folic acid-linked poly(ethylene glycol) (PEG-FA) were synthesized for cancer theranostic applications. In the developed NPs, the DOX was chemically conjugated at the surface of core-shell Fe3O4@Au NPs using L-cysteine methyl ester (LCME) as a linker by acid-sensitive hydrazone bond. The formation of Fe3O4@Au-DOX-mPEG/PEG-FA NPs was c...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Xiaobing Yu, Wei Huang, Dewei Zhao, Ke Yang, Lili Tan, Xiuzhi Zhang, Junlei Li, Meng Zhang, Shuo Zhang, Tao Liu, Baolin Wu, Mingjia Qu, Ruimeng Duan, Yashuai YuanAbstractLow molecular weight poly-lactic acid (PLLA) is a polymer matrix of orthopaedic implants. The PLLA matrix incorporating bioactive magnesium ion (Mg2+) enhances bone regeneration. But the optimal ratio of Mg2+ to PLLA matrix has not been well reported and is worthy of study. We synthesized silane-coated Mg/PLLA composites containing 1%, 2%, 3%, 4% and 5% Mg...
Source: Colloids and Surfaces B: Biointerfaces - November 21, 2018 Category: Biochemistry Source Type: research

Laser writing of nanostructured silicon arrays for the SERS detection of biomolecules with inhibited oxidation
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): P. Pellacani, V. Torres-Costa, F. Agulló-Rueda, R. Vanna, C. Morasso, M. Manso SilvánAbstractThe present work reports the processing of laser irradiated Si arrays (LISi) and underlines their surface enhanced Raman scattering (SERS) functionality. A nanostructured Si/SiOx surface forms providing additional fluidic and photoprotective properties. Because of their optical and surface characteristics, the arrays exhibit a SERS analytical enhancing factor of 500, without any noble metals such as gold or silver. Mi...
Source: Colloids and Surfaces B: Biointerfaces - November 17, 2018 Category: Biochemistry Source Type: research

Red blood cell adhesion can be reduced by non-reactive macromolecules
This study thus suggests that depletion interaction can control cell-cell interactions in complex environments (e.g., in vivo), and indicates that considering the interplay of all plasma constituents is important in order to understand the pathophysiology of diseases associated with cell adhesion and vascular complications.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - November 17, 2018 Category: Biochemistry Source Type: research

Prediction of residual film perception of cosmetic products using an instrumental method and non-biological surfaces: The example of stickiness after skin application
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Florine Eudier, Déborah Hirel, Michel Grisel, Céline Picard, Géraldine SavaryAbstractPrediction of sensory texture attributes using instrumental measurements is a very important challenge for cosmetic industry because in vivo sensory studies are expensive, time consuming and limited by the safety issue of applied products. The aim of this work is to investigate how residual sensory properties of cosmetic products can be predicted without using a panel of assessors, focusing on the residual film attribu...
Source: Colloids and Surfaces B: Biointerfaces - November 17, 2018 Category: Biochemistry Source Type: research

Synthesis and characterization of magnetic hybrid nanomaterials via RAFT polymerization: A pH sensitive drug delivery system
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Ali Pourjavadi, Mohammad Kohestanian, Mahdieh ShirzadAbstractHerein, a facile and versatile method for the synthesis of a novel magnetic nanocarrier via surface- initiated reversible addition-fragmentation chain transfer (RAFT) polymerization is introduced. At first, RAFT agent was successfully attached to the surface of Fe3O4 nanoparticles and, then, poly (glycidyl methacrylate) (PGMA) chains were grown and anchored onto the surface of Fe3O4 nanoparticles. At the end, hydrazine (Hy) groups were introduced to the PGMA chai...
Source: Colloids and Surfaces B: Biointerfaces - November 16, 2018 Category: Biochemistry Source Type: research

Effect of a lipopeptide biosurfactant on the precipitation of calcium carbonate
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Anna Bastrzyk, Marta Fiedot-Toboła, Izabela Polowczyk, Krzysztof Legawiec, Grażyna PłazaAbstractObserving flora and fauna, it can be said that nature is a great architect. Nature can create amazing structures with unique properties that may find potential applications in industry. This phenomenon is why the biomimetic synthesis of calcium carbonate with various polymorphs, sizes and morphologies using natural biomolecules, such as proteins and polysaccharides, has become an interesting topic in recent years. This novel ...
Source: Colloids and Surfaces B: Biointerfaces - November 16, 2018 Category: Biochemistry Source Type: research

PEG-b-poly (carbonate)-derived nanocarrier platform with pH-responsive properties for pancreatic cancer combination therapy
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Priyanka Ray, Matthew Confeld, Pawel Borowicz, Tao Wang, Sanku Mallik, Mohiuddin QuadirAbstractA pH-responsive nanoparticle platform, based on PEG-b-poly (carbonate) block copolymers have been proposed that can respond to low pH as found in many cancer micro- and intracellular environment, including that in pancreatic cancer. The hydrophobic domain, i.e., the poly (carbonate) segment has been substituted with tertiary amine side chains, such as N, Nʹ-dibutylethylenediamine (pKa = 4.0, DB) and 2-pyrrolidin-1-yl-ethyl-amine...
Source: Colloids and Surfaces B: Biointerfaces - November 16, 2018 Category: Biochemistry Source Type: research

Human dermal fibroblast proliferation controlled by surface roughness of two-component nanostructured latex polymer coatings
In this study hierarchically-structured latex polymer coatings and self-supporting films were characterised and their suitability for cell growth studies was tested with Human Dermal Fibroblasts (HDF). Latex can be coated or printed on rigid or flexible substrates thus enabling high-throughput fabrication. Here, coverslip glass substrates were coated with blends of two different aqueous latex dispersions: hydrophobic polystyrene (PS) and hydrophilic carboxylated acrylonitrile butadiene styrene (ABS). The nanostructured morphology and topography of the latex films was controlled by varying the mixing ratio of the components...
Source: Colloids and Surfaces B: Biointerfaces - November 16, 2018 Category: Biochemistry Source Type: research

Novel Enteromorpha Prolifera based carbon dots: Probing the radical scavenging of natural phenolic compounds
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Xiaojuan Wang, Qi Chen, Zhengyuan Zhang, Hua He, Xiqi Ma, Zhenzhen Liu, Baosheng Ge, Fang HuangAbstractPhenolic compounds can act as protective agents against free radical oxidation. It is desirable to develop simple and efficient analytical techniques to measure the phenolic content and their antioxidant capacity. Using Enteromorpha prolifera (EP) as the carbon source, novel fluorescent carbon dots (EP-CDs) were synthesized through a facile microwave-assisted hydrothermal method. EP-CDs exhibit high mono-dispersity in aqu...
Source: Colloids and Surfaces B: Biointerfaces - November 16, 2018 Category: Biochemistry Source Type: research

Biofilms and vulvovaginal candidiasis
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Carmen Rodríguez-Cerdeira, Miguel Carnero Gregorio, Alberto Molares-Vila, Adriana López-Barcenas, Gabriella Fabbrocini, Brunilda Bardhi, Ardiana Sinani, Elena Sánchez-Blanco, Roberto Arenas-Guzmán, Rigoberto Hernandez-CastroAbstractCandida species, including C. albicans, are part of the mucosal flora of most healthy women, and inhabit the gastrointestinal and genitourinary tracts. Under favourable conditions, they can colonize the vulvovaginal mucosa, giving rise to symptomatic vulvovaginal cand...
Source: Colloids and Surfaces B: Biointerfaces - November 15, 2018 Category: Biochemistry Source Type: research

SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Weronika Karawacka, Christina Janko, Harald Unterweger, Marina Mühlberger, Stefan Lyer, Nicola Taccardi, Andriy Mokhir, Wolfgang Jira, Wolfgang Peukert, Aldo R. Boccaccini, Mikhail Kolot, Richard Strauss, Christian Bogdan, Christoph Alexiou, Rainer TietzeAbstractSystemic inflammation such as sepsis represents an acute life-threatening condition, to which often no timely remedy can be found. A promising strategy may be to functionalize magnetic nanoparticles with specific peptides, derived from the binding motives of a...
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Isoelectric point of free and adsorbed cytochrome c determined by various methods
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Svetlana H. Hristova, Alexandar M. ZhivkovAbstractThe purpose is to determine the isoelectric point (IEP) pIμ of cytochrome c (cytC, a globular haemoproteid) adsorbed on montmorillonite (MM, plate-like colloid particles) by microelectrophoresis and to compare the pIμ value with pIf9.44 measured by isoelectric focusing in gel with covalently linked ampholytes, and with pInz10.0–10.6 of free cytC globule calculated by three programs for pH-dependent net charge nz using the crystallographic structure of cytC. The ...
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Influence of hybrid polymeric nanoparticle/thermosensitive hydrogels systems on formulation tracking and in vitro artificial membrane permeation: A promising system for skin drug-delivery
In this study, benzocaine-loaded poly-ε-caprolactone nanoparticles (BZC:NPs) were synthesized, characterized and incorporated into Poloxamer 407-based hydrogel (PL407). Benzocaine (BZC) was used as a drug model since has been commonly applied as a topical pain reliever in the last years. Hence, we developed a hybrid polymeric nanoparticle/thermosensitive hydrogels system and evaluated the in vitro permeation of the BZC, as well as nanoformulation tracking in an artificial membrane. In vitro permeation study was conducted in a vertical diffusion cell system using a Strat-M® membrane model. BZC:NPs were prepared ...
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Zeta potential changes of Saccharomyces cerevisiae during fermentative and respiratory cycles
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Lucía M. Lavaisse, Axel Hollmann, Mónica A. Nazareno, Edgardo A. DisalvoAbstractSaccharomyces cerevisiae is a type of yeast, widely used in diverse biotechnological food-beverage processes. Although the performance of an industrial fermentation process depends largely on the number of cells, it is necessary to consider the physiological state of the cultures. In this context, the aim of this study was to determine in a yeast culture how factors such as growth conditions affect surface properties at the differ...
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Magnetic bioinspired micro/nanostructured composite scaffold for bone regeneration
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Yao Zhao, Tiantang Fan, Jingdi Chen, Jiacan Su, Xin Zhi, Panpan Pan, Lin Zou, Qiqing ZhangAbstractMagnetic-responsive materials are promising for applications in various biomedical fields. Especially, superparamagnetic nanoparticles are widely used in magnetic system for bone tissue engineering owing to superior biocompatibility and long term stability. Based on the idea of in situ bionics, we successfully incorporate the nano-hydroxyapatite (nHAP) and Fe3O4 nanoparticles which were prepared by in situ crystallization and ...
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Enhanced anti-tumor efficacy and reduced cardiotoxicity of doxorubicin delivered in a novel plant virus nanoparticle
This study is the first report on applicability of JgCSMV for Dox delivery with superior benefits over generally marketed formulations of doxorubicin.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Contractile behaviors of cardiac muscle cells on mushroom-shaped micropillar arrays
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Nomin-Erdene Oyunbaatar, Arunkumar Shanmugasundaram, Dong-Weon LeeAbstractIn this work we propose mushroom-shaped PDMS (Polydimethylsiloxane) μpillar arrays for enhancing the contractile force of cardiomyocytes during cell culturing. Conventional micropillar (μpillar) arrays with flat surfaces were employed as a standard sample to quantitatively recognize experimental data and to conclusively demonstrate the improved performance of mushroom-shaped PDMS μpillar arrays. Cardiomyocytes isolated from experimental anim...
Source: Colloids and Surfaces B: Biointerfaces - November 14, 2018 Category: Biochemistry Source Type: research

Recent advances in magnetic fluid hyperthermia for cancer therapy
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Pradip Das, Miriam Colombo, Davide ProsperiAbstractRecently, magnetic fluid hyperthermia using biocompatible magnetic nanoparticles as heat mediators for cancer therapy has been extensively investigated due to its high efficiency and limited side effects. However, the development of more efficient heat nanomediators that exhibit very high specific absorption rate (SAR) value is essential for clinical application to overcome the several restrictions previously encountered due to the large quantity of nanomaterial required f...
Source: Colloids and Surfaces B: Biointerfaces - November 12, 2018 Category: Biochemistry Source Type: research

Polydopamine reinforced hemostasis of a graphene oxide sponge via enhanced platelet stimulation
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Guofeng Li, Yuping Liang, Congcong Xu, Hui Sun, Lei Tao, Yen Wei, Xing WangAbstractGraphene oxide (GO) is a promising hemostatic material because of its platelet stimulatory activity. However, our previous studies on cross-linked graphene sponges demonstrated that those sponges lost the GO function of platelet stimulation due to the pristine GO was reduced under the harsh reaction conditions. Accordingly, a mild cross-linking strategy is expected to preserve the oxygen-containing groups to further increase the hemostatic p...
Source: Colloids and Surfaces B: Biointerfaces - November 9, 2018 Category: Biochemistry Source Type: research

Reversing multi-drug tumor resistance to Paclitaxel by well-defined pH-sensitive amphiphilic polypeptide block copolymers via induction of lysosomal membrane permeabilization
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Hassan Mostoufi, Gholamhossein Yousefi, Ali-mohammad Tamaddon, Omidreza FiruziAbstractA series of hybrid di-block copolymers of poly(l- glutamic acid-b-l- leucine) (PGA-PLeu), methoxy poly (ethylene glycol)-b-poly(l-leucine) (PEG-PLeu), methoxy poly(ethylene glycol)-b-poly(γ-benzyl-l-glutamic acid) (PEG-PBLG) and tri-block copolymers of poly(ethylene glycol)-b-poly(l-glutamic acid-co-null-leucine) (PEG-PGA-PLeu) were synthesized through sequential HMDS-mediated ring-opening polymerization (ROP). Chemical structure of...
Source: Colloids and Surfaces B: Biointerfaces - November 6, 2018 Category: Biochemistry Source Type: research

The effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactions
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): F. Romero-Gavilán, N. Araújo-Gomes, I. García-Arnáez, C. Martínez-Ramos, F. Elortza, M. Azkargorta, I. Iloro, M. Gurruchaga, J. Suay, I. GoñiAbstractIt is known strontium can both inhibit the osteoclast formation and stimulate the osteoblast maturation, so biomaterials containing this element can favour bone structure stabilisation. The addition of Sr to biomaterials could affect their interactions with proteins and cells. Here, a silica-hybrid sol-gel network doped with different ...
Source: Colloids and Surfaces B: Biointerfaces - November 6, 2018 Category: Biochemistry Source Type: research

Antibiofilm efficacy of curcumin in combination with 2-aminobenzimidazole against single- and mixed-species biofilms of Candida albicans and Staphylococcus aureus
In this study, we evaluated the 2-aminobenzimidazole (2ABI) and curcumin (CUR) alone to inhibit planktonic cell growth, adhesion as well as single and mixed species biofilms of Candida albicans and Staphylococcus aureus on silicone. The combined effects between 2ABI and CUR on mixed species biofilm formation and pre-formed biofilm were assessed. Our work showed that 2ABI or CUR alone was effective as a sole agent, inhibiting planktonic growth, adhesion and the biofilm formation of bacteria and fungi on the silicone surface. The combination of 2ABI and CUR exhibited the enhanced effect on mixed biofilm compared to mono-drug...
Source: Colloids and Surfaces B: Biointerfaces - November 6, 2018 Category: Biochemistry Source Type: research

The preparation of the ordered pores colloidal crystal scaffold and its role in promoting growth of lung cells
In this study, chitosan and gelatin as natural biological macromolecules raw material for the synthesis of ordered colloidal crystal scaffolds (CCS), Fe3O4 magnetic nanoparticles (MNPs) were used as pore-making for the first time. The pore-making agent were added into the hydrogels to synthesis the ordered (magnetic field) and disordered (no magnetic field) CCS. Collagen and basic fibroblast growth factor (bFGF) modified on the surface of CCS. Then mouse lung epithelial cells (TC-1) and normal human bronchial epithelial cells (Beas-2B) were cultured on the scaffold, obviously induced cell proliferation. Various physical an...
Source: Colloids and Surfaces B: Biointerfaces - November 4, 2018 Category: Biochemistry Source Type: research

Amphiphilic quaternary ammonium chitosans self-assemble onto bacterial and fungal biofilms and kill adherent microorganisms
Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Joonhoo Jung, Jianchun Wen, Yuyu SunAbstractFormation of biofilms on solid surfaces is a long-standing challenge to multiple medical and health-related applications. Once formed, biofilms are very difficult to destroy, and microorganisms in biofilms are much more resistant to antimicrobial agents than their free-floating counterparts. The current antimicrobial agents/disinfectants often have low residence time on biofilms, leading to low antimicrobial effect on biofilm microorganisms. We designed and synthesized an amphiph...
Source: Colloids and Surfaces B: Biointerfaces - November 4, 2018 Category: Biochemistry Source Type: research

The use of biovesicles to improve the efficiency of Zn foliar fertilization
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Juan José Rios, Paula Garcia-Ibañez, Micaela CarvajalAbstractFoliar fertilization is becoming very popular since it is highly efficient, minimizes contamination, and is target oriented. However, the low mobility of several nutrients and low penetration through the leaf cuticle should be addressed, to establish an efficient leaf applied fertilizer. During the last few years, nanotechnologies – such as nanocarriers - have been studied with regard to improving the penetration and delivery of compounds. This...
Source: Colloids and Surfaces B: Biointerfaces - November 3, 2018 Category: Biochemistry Source Type: research

Recent advances in nano-bio-interactions: Editorial
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Sami Rtimi, Paul Bowen, Alke Petri-Fink (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - November 3, 2018 Category: Biochemistry Source Type: research

The bio-interface between functionalized Au NR@GO nanoplatforms with protein corona and their impact on delivery and release system
In conclusion, modification of nanoplatforms with targeting agents could alter the composition of corona toward well interaction with cell and deliver the therapeutic agent.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - November 2, 2018 Category: Biochemistry Source Type: research

The “Race for the Surface” experimentally studied: In vitro assessment of Staphylococcus spp. adhesion and preosteoblastic cells integration to doped Ti-6Al-4V alloys
ConclusionTo our knowledge, this is the first time that anin vitro study emulating the race for the surface evaluates and compares the osseointegration and antibacterial properties between two nanostructured- modified titanium alloy surfaces. Clinical strains show different behavior from collection ones in bacterial adherence. The presence of cells increased bacterial adherence. NP and NT surface modifications didn´t show significant differences in bacterial adhesion and preosteoblastic cells integration.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - November 1, 2018 Category: Biochemistry Source Type: research

Surface structure and volatile characteristic of peanut proteins obtained through AOT reverse micelles
This study provided the investigation of the surface structure and volatile compounds of peanut proteins obtained through aqueous buffer (AB) and reverse micelles (RMs) by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and gas chromatography–mass spectrometry (GC–MS). The results showed that RMs could modify the amorphous structure of peanut proteins and change the original structure. Significant differences were between the C, O, and N content in two type protein surfaces (P 
Source: Colloids and Surfaces B: Biointerfaces - November 1, 2018 Category: Biochemistry Source Type: research

Low-temperature immobilization of water in Antarctic Turgidosculum complicatulum and in Prasiola crispa. Part I. Turgidosculum complicatulum
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): M. Bacior, H. Harańczyk, P. Nowak, P. Kijak, M. Marzec, J. Fitas, M.A. OlechAbstractThe studies of low-temperature immobilization of bound water in Antarctic lichenized fungus Turgidosculum complicatulum were performed using 1H NMR and DSC over a wide range of thallus hydration. 1H NMR free induction decays were decomposed into a solid component well described by the Gaussian function and two exponentially decaying components coming from a tightly bound water and from a loosely bound water fraction. 1H NMR spectra revealed...
Source: Colloids and Surfaces B: Biointerfaces - November 1, 2018 Category: Biochemistry Source Type: research

Poly(ε-caprolactone) modified organic dyes nanoparticles for noninvasive long term fluorescence imaging
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Li Quan, Tingting Sun, Yanchun Wei, Yuebin Lin, Tao Gong, Changjiang Pan, Hongyan Ding, Wei Liu, Zhigang XieAbstractApplication of organic dyes is limited in biomedical fields due to the rapid self-quenching, poor stability and water solubility. In this work, polymer modified organic dyes is developed through the dyes initiating polymerization. The polymer length is studied by tuning the amount of monomer. With an optimal molecule weight of the polymer, the π–π stacking from π-conjugated organic fluorescent d...
Source: Colloids and Surfaces B: Biointerfaces - November 1, 2018 Category: Biochemistry Source Type: research

Magnetofluorescent nanohybrid comprising polyglycerol grafted carbon dots and iron oxides: Colloidal synthesis and applications in cellular imaging and magnetically enhanced drug delivery
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Yu Wen, Meiyun Xu, Xin Liu, Xiaoya Jin, Jiaqi Kang, Di Xu, Houyi Sang, Peng Gao, Xiao Chen, Li ZhaoAbstractMultifunctional nanohybrids are attracting increasing attention for potential biomedical applications such as bioimaging and drug delivery due to their combined advantages of individual components. However, challenges in the improvement of their synthesis and colloidal stability to facilitate practical biomedical applications still remain. In this work, we report an efficient synthetic approach to fabricate magnetofluo...
Source: Colloids and Surfaces B: Biointerfaces - October 31, 2018 Category: Biochemistry Source Type: research

Nanoencapsulation of the flavonoid dihydromyricetin protects against the genotoxicity and cytotoxicity induced by cationic nanocapsules
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Ana Julia F. Dalcin, Bruno S. Vizzotto, Guilherme V. Bochi, Naiara S. Guarda, Kátia Nascimento, Michele R. Sagrillo, Rafael N. Moresco, André P. Schuch, Aline F. Ourique, Patrícia GomesAbstractWe evaluated the influence of nanoencapsulation of the flavonoid Dihydromyricetin (DMY) in reducing the genotoxicity and cytotoxicity induced by cationic nanocapsules. Assays were conducted in order to evaluate the potential of protein corona formation, cytotoxicity, genotoxicity and the antioxidant capacity. Nano...
Source: Colloids and Surfaces B: Biointerfaces - October 31, 2018 Category: Biochemistry Source Type: research

Axial Suspension Plasma Spraying: An ultimate technique to tailor Ti6Al4V surface with HAp for orthopaedic applications
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Pearlin Hameed, Vasanth Gopal, Stefan Bjorklund, Ashish Ganvir, Dwaipayan Sen, Nicolaie Markocsan, Geetha ManivasagamAbstractDissolution of atmospheric plasma sprayed (APS) hydroxyapatite (HAp) coatings on Ti-6Al-4 V medical implants have always been a challenge to overcome in the field of biomedical industry. In the present work, an attempt has been made to develop a HAp coating using a novel thermal spray process called axial suspension plasma spraying (SPS), which leads to thin adherent coatings. Two HAp coatings ...
Source: Colloids and Surfaces B: Biointerfaces - October 31, 2018 Category: Biochemistry Source Type: research

Surface-immobilized plant-derived osteopontin as an effective platform to promote osteoblast adhesion and differentiation
In this report, recombinant human osteopontin synthesized in tobacco plants (p-rhOPN) is introduced as a potential bioactive molecule that can promote osteoblast adhesion and differentiation. A glass substrate (SiO2/Si-OH) grafted with poly(acrylic acid) (SiO2/Si-PAA) was prepared by surface-initiated reversible addition-fragmentation chain transfer polymerization and used as a carboxyl-rich platform for the chemical conjugation of p-rhOPN. The PAA grafting and subsequent p-rhOPN immobilization were confirmed by water contact angle, Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and atomic force ...
Source: Colloids and Surfaces B: Biointerfaces - October 31, 2018 Category: Biochemistry Source Type: research

Light-induced mechanisms for nanocarrier’s cargo release
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Ana Sanchis, J.-Pablo Salvador, M.-Pilar MarcoAbstractNanomaterials have been the focus of attention in several fields, including biomedicine, electronics, or catalysis, mainly due to the novel properties of the materials at the nanoscale. In the field of diagnosis, nanomaterials have been contemplated as an opportunity to improve sensitivity and time of response, therefore, facilitating early treatment and monitoring of the disease. For therapeutic applications, new drug delivery nanosystems aiming to provide enhanced effi...
Source: Colloids and Surfaces B: Biointerfaces - October 31, 2018 Category: Biochemistry Source Type: research