Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay
Publication date: 1 April 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 176Author(s): Pavel Khramtsov, Maria Kropaneva, Il’ya Byzov, Artem Minin, Alexey Mysik, Valeria Timganova, Maria Bochkova, Mikhail Uimin, Svetlana Zamorina, Anatoly Yermakov, Mikhail RayevAbstractIn this work, we developed and optimized conjugates of carbon-coated iron nanoparticles (Fe@C) with streptavidin and monoclonal antibodies. The conjugation procedure included two stages. First, amino groups were grafted onto the carbon shell to facilitate noncovalent sorption of bovine serum albumin (BSA). Further, the covalent attachment of...
Source: Colloids and Surfaces B: Biointerfaces - January 9, 2019 Category: Biochemistry Source Type: research

Extracting lipid vesicles from plasma membranes via self-assembly of clathrin-inspired scaffolding nanoparticles
Publication date: 1 April 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 176Author(s): Ye Li, Xianren Zhang, Jinxing Lin, Ruili Li, Tongtao YueAbstractSingle-cell analysis is a new and rapidly expanding field, the goal of which is obtaining fresh information from individual cells to understand the regulatory mechanisms of cell development and diseases. Conventional approaches generally rely on the cell lysis which, however, is destructive to cells and against multiple sampling from the living cell. Here, we propose and design a scaffolding nanoparticle (NP) system that enables us to sample cytoplasmic contents ...
Source: Colloids and Surfaces B: Biointerfaces - January 9, 2019 Category: Biochemistry Source Type: research

Spectroscopic and mechanistic analysis of the interaction between Jack bean urease and polypseudorotaxane fabricated with bis-thiolated poly(ethylene glycol) and α-cyclodextrin
Publication date: 1 April 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 176Author(s): Jun Zhao, Chun-Liu Yu, Wei Fang, Ji-Duan Lin, Guo Chen, Xiao-Qin WangAbstractSelf-assembled polypseudorotaxanes (PPRXs) fabricated with α-cyclodextrin and poly(ethylene glycol) (PEG) or its thiolated derivatives were candidate functional materials for enzyme soft-immobilization, encapsulation and controlled-release. The study of their interaction with Jack bean urease (JBU) indicated that they inconspicuously influenced the activity and stability of JBU during long storage, up to 30 days. The macro-species were inaccess...
Source: Colloids and Surfaces B: Biointerfaces - January 9, 2019 Category: Biochemistry Source Type: research

Novel scheme for rapid synthesis of hollow mesoporous silica nanoparticles (HMSNs) and their application as an efficient delivery carrier for oral bioavailability improvement of poorly water-soluble BCS type II drugs
This study, therefore, offered a new, simple, and quick method to develop HMSNs with the ability to support higher loading and controlled release behavior in vitro and enhanced absorption of poorly-aqueous soluble drugs in-vivo.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - January 5, 2019 Category: Biochemistry Source Type: research

Injectable and biodegradable phospholipid-based phase separation gel for sustained delivery of insulin
In conclusion, PPSG is of good biocompatibility and biodegradability, which is promising to serve as a sustained insulin delivery system and improve patient compliance effectively.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - January 5, 2019 Category: Biochemistry Source Type: research

Liposomes with Temperature-Responsive Reversible Surface Properties
Publication date: Available online 3 January 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Ryo Nemoto, Kei Fujieda, Yuki Hiruta, Mafumi Hishida, Eri Ayano, Yoshie Maitani, Kenichi Nagase, Hideko KanazawaAbstractLiposomes composed of egg phosphatidylcholine and cholesterol were modified with the temperature-responsive polymer poly(N-isopropylacrylamide-co-N, N-dimethylacrylamide) (P(NIPAAm-co-DMAAm)), and exhibited reversible surface properties with temperature. Completely reversible liposome aggregation due to P(NIPAAm-co-DMAAm) hydration/dehydration was demonstrated over four successive cycles of heating an...
Source: Colloids and Surfaces B: Biointerfaces - January 3, 2019 Category: Biochemistry Source Type: research

Transferrin-conjugated pH-sensitive platform for effective delivery of porous palladium nanoparticles and paclitaxel in cancer treatment
In this study, a transferrin-conjugated pH-sensitive platform (Tf-PPP), comprising porous Pd nanoparticles (PdNPs) and paclitaxel (PTX), was successfully developed for combined chemo-phototherapy. Tf-PPPs have a small size of 164.6 ± 8.7 nm, PDI of 0.278 ± 0.029, and negative charge (-13.2 ± 1.8 mV). Poly(acrylic acid)-poly(ethylene oxide) (PAA-PEO), a pH sensitive polymer, was used to achieve pH-dependent drug release from nanoparticles. Transferrin (Tf) conjugated on the surface of nanoplatforms could enhance the cellular uptake and prolong nano...
Source: Colloids and Surfaces B: Biointerfaces - January 3, 2019 Category: Biochemistry Source Type: research

Mesoporous Silica-Coated Gold Nanoframes as Drug Delivery System for Remotely Controllable Chemo-Photothermal Combination Therapy
Publication date: Available online 3 January 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zhiguo Gao, Tianyi Shi, Yaojia Li, Chaoqun You, Kaiwu Cheng, Baiwang SunAbstractTumor cells experience higher chemotherapy stress under condition of elevated temperature. As a result, developing novel nanoagents that integrates chemotherapy and thermotherapy holds great promise in biomedicine. Herein, utilizing spatially confined galvanic replacement method, we fabricated a yolk-shell Au@mSiO2 nanoframes with Au NPs and mesoporous silica as yolk and shell, respectively, to sever as an excellent drug nanocarrier with ef...
Source: Colloids and Surfaces B: Biointerfaces - January 3, 2019 Category: Biochemistry Source Type: research

Near infrared light-responsive heat-emitting hemoglobin hydrogels for photothermal cancer therapy
Publication date: Available online 31 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Changkyu Lee, Kyungseop Lim, Sung Soo Kim, Le Xuan Thien, Eun Seong Lee, Kyung Taek Oh, Han-Gon Choi, Yu Seok YounAbstractPhotothermal therapy (PTT) is an effective means of treating tumors because tumor cells are sensitive to heat. Gold and carbon nanoparticles are used as efficient PTT materials. However, development of a non-toxic biodegradable PTT agent remains a challenge. Here, we developed a hemoglobin (Hb) hydrogel that exhibited excellent PTT effects in vitro and in vivo. Unlike conventional PTT agents, whic...
Source: Colloids and Surfaces B: Biointerfaces - December 31, 2018 Category: Biochemistry Source Type: research

Interaction of Methylparaben and Propylparaben with P123/F127 Mixed Polymeric Micelles
Publication date: Available online 30 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): M. Senthilkumar, Sasmita DashAbstractThis paper reports the interaction between Pluronic F127, P123 and their mixed micelles with methylparaben and propylparaben. The UV studies revealed that, the spectral behavior of mixed micelle of F127 and P123 with parabens lay between their individual micellar behaviors. The cloud point studies have shown similar results. It was observed that the intensity of fluorescence spectra, compared with the single micelle-drug combinations was much higher with the mixed micelle-methylpa...
Source: Colloids and Surfaces B: Biointerfaces - December 31, 2018 Category: Biochemistry Source Type: research

Electrooptical determination of the isoelectric point of globular proteins: cytochrome c adsorbed on montmorillonite nanoplates
Publication date: Available online 30 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Svetlana H. Hristova, Alexandar M. ZhivkovAbstractThe purpose is to study the capability of the electric light scattering to determine the point of zero charge of native protein macromolecules adsorbed on colloid particles at low ionic strength and without using buffers. The chosen protein and particles are cytochrome c (cytC, globular haemoproteid) and montmorillonite (MM, negatively charged plate-like crystal). The pH-dependence of the electric polarizability γ(pH) in the range pH 6–11 shows minimum whi...
Source: Colloids and Surfaces B: Biointerfaces - December 31, 2018 Category: Biochemistry Source Type: research

Antibacterial Silk Fibroin Scaffolds with Green Synthesized Silver Nanoparticles for Osteoblast Proliferation and Human Mesenchymal Stem Cell Differentiation
We report here, a silk fibroin based bone tissue engineered scaffold with AgNPs having advanced antimicrobial properties, without compromising its cytocompatibility and stem cell differentiation potential. For this purpose, AgNPs were in situ synthesized using silk fibroin as reducing as well as stabilizing agent. The antimicrobial activity of silk fibroin films with AgNPs was evaluated against gram negative bacteria as well as antibiotic resistant bacteria and it was found to be effective against both. The cytocompatibility of these scaffolds was examined with fibroblast and osteoblast cells. Also the effect of AgNPs pres...
Source: Colloids and Surfaces B: Biointerfaces - December 28, 2018 Category: Biochemistry Source Type: research

The On-Off chiral mesoporous silica nanoparticles for delivering achiral drug in chiral environment
Publication date: Available online 26 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Na Fan, Rongzheng Liu, Pingping Ma, Xin Wang, Chang Li, Jing LiAbstractIn the current stage, the On-Off mechanism of chiral mesoporous silica nanoparticles (CMSN) for delivering achiral drug in chiral environment has rarely been reported. Herein, On-Off chiral mesoporous silica nanoparticles (On-Off-D-CMSN and On-Off-L-CMSN) were successfully synthesized and its particular contribution in delivering achiral drug indometacin (IMC) in chiral environment was mainly studied. The as-synthesized On-Off-D-CMSN and On-Off-L-...
Source: Colloids and Surfaces B: Biointerfaces - December 27, 2018 Category: Biochemistry Source Type: research

Cell Response to Plasma Electrolytic Oxidation Surface-Modified Low-Modulus β-Type Titanium Alloys
This study shows that PEO technology can be used to modify the surface of low-modulus β-type titanium alloys with porous structure facilitating osseointegration, without impeding osteoblast activity or introducing an untoward inflammatory response.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - December 27, 2018 Category: Biochemistry Source Type: research

Dissecting the effects of Free Fatty Acids on the Thermodynamic Stability of Complex Model Membranes mimicking Insulin Secretory Granules
Publication date: Available online 27 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Francesca Saitta, Marco Signorelli, Dimitrios FessasAbstractA stepwise micro-DSC study of Small, Large and Giant Unilamellar Vesicles prepared as pure and mixed systems of DMPC, DPPC, DSPC and DOPC was performed, achieving the preparation of final model membranes whose phospholipid compositions represent the 75% in terms of the phospholipids tails and the 50% headgroups of the Insulin Secretory Granules (vesicles located in the pancreatic Langerhans β-cells and which are responsible for insulin and amylin storag...
Source: Colloids and Surfaces B: Biointerfaces - December 27, 2018 Category: Biochemistry Source Type: research

Thiabendazole/bentonites hybrids as controlled release systems
Publication date: Available online 13 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Graycyelle R.S. Cavalcanti, Maria G. Fonseca, Edson C. da Silva Filho, Maguy JaberAbstractClay minerals are commonly used in pharmaceutical products as excipients and active agents. New drug vehicles based on clay minerals have been developed. In this work, sodium (BentNa), calcium (BentCa) and magnesium (BentMg) exchanged bentonites were used for the sorption of thiabendazole (TBZ), and their potential use as controlled release systems was evaluated. Pristine bentonite and exchanged bentonites were characterized by ...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Karin Luize Mazur, Paulo Emílio Feuser, Alexsandra Valério, Arthur Poester Cordeiro, Camila Indiani de Oliveira, João Paulo Assolini, Wander Rogério Pavanelli, Claudia Sayer, Pedro H.H. AraújoAbstractLeishmaniasis is considered a neglected tropical disease that represents a Public Health problem due to its high incidence. In the search of new alternatives for Leishmaniasis treatment diethyldithiocarbamate (DETC) has shown an excellent leishmanicidal activity and the incorporation in...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Biomimetic modification of poly-l-lysine and electrodeposition of nanocomposite coatings for orthopaedic applications
Publication date: Available online 19 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): A. Clifford, B.E.J. Lee, K. Grandfield, I. ZhitomirskyAbstractFor the first time, a biomimetic method has been developed for the chemical modification of poly-l-lysine (PLL) with catechol in order to improve polymer adhesion to inorganic particles and surfaces. The method is based on the Schiff base reaction of amino groups of PLL monomers and aldehyde groups of 3,4-dihydroxybenzylaldehyde (DHBA) molecules. It was found that adherent PLL-DHBA films can be prepared by cathodic electrophoretic deposition (EPD). Nanocom...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

A hemocompatible cryoprotectant inspired by freezing-tolerant plants
Publication date: Available online 19 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jing Yang, Xiaojie Sui, Chiyu Wen, Chao Pan, Yingnan Zhu, Jiamin Zhang, Lei ZhangAbstractCryopreservation can extend the storage time of red blood cells (RBCs) for even decades, offering a promising solution to blood waste and shortage caused by routinely used hypothermic preservation method (~42 days). Currently, organic solvents such as glycerol or dimethyl sulfoxide are the state-of-the-art cryoprotectants (CPAs). However, severe RBC hemolysis induced by solvent CPA removal has raised serious concerns and has been...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

DNA G-quadruplexes binding and antitumor activity of palladium aryl oxime ligand complexes encapsulated in either albumin or algal cellulose nanoparticles
Publication date: Available online 19 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Nazanin Hashemzadeh Esfahani, Fatemeh Salami, Zeinab Saberi, Kazem Karami, Zohreh Mehri Lighvan, Mohammad Ramezani, Mona Alibolandi, Sara Amel Farzad, Taghi KhayamianAbstractThe interactions between two Pd complexes, designated as [Pd3(C,N-(C6H4C(Cl)=NO)-4)6] (complex 1) and [Pd3(C12H8C = NO)6] (complex 2), with the human telomeric G-quadruplex DNA, 5′-G3(T2AG3)3-3′ (HTG21), were monitored using spectroscopic, biological, and molecular modeling studies. According to the UV-Vis results, these...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Responsive Coatings from Naturally Occurring Pectin Polysaccharides
Publication date: Available online 21 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zeinab Veisi, Nathan D. Gallant, Norma A. Alcantar, Ryan G. ToomeyAbstractPectin polysaccharides have significant potential as all-natural, non-toxic “green” coatings that exhibit thermally-cued swelling behavior. Herein, ultra-thin coatings of highly-esterified pectin polysaccharides were cross-linked with calcium chloride (CaCl2) and their swelling in water was investigated with ellipsometry. At low temperatures, the coatings swell to 2-3 times their dry layer thickness. As the temperature is increased,...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Spectroscopic Ellipsometry of fluid and gel phase Lipid Bilayers in hydrated conditions
Publication date: Available online 21 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sagar Kamble, Snehal Patil, Mandar Kulkarni, Appala Venkata Ramana MurthyAbstractThe biological membranes play a crucial role in the various biological processes due to their characteristic physical properties. The parameters such as membrane composition, thickness undulations, and the influence of external stimuli play a crucial role in the phase state behavior of biological membranes. The supported lipid bilayer (SLBs) systems closely represent cell membranes and are often studied to understand their behavior. In t...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Ultrafast fabrication of thermally stable protein-coated silver iodide nanoparticles for solid-state superionic conductors
Publication date: Available online 21 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Aryane Tofanello, Juscemácia N. Araujo, Iseli L. Nantes-Cardoso, Fabio F. Ferreira, José A. Souza, Dae-Woon Lim, Hiroshi Kitagawa, Wanius GarciaAbstractSolid-state ionic conductor is an essential and critical part of electrochemical devices such as batteries and sensors. Nano-sized silver iodide (AgI) is the most promising ionic conductor due to its superionic conductivity at room temperature. In recent years, proteins have been used as organic templates to obtain high-performance solid-state ionic cond...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Magnetic iron oxide nanoparticles modified with vanadate and phosphate salts for purification of alkaline phosphatase from the bovine skim milk
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Hamed Farzi-Khajeh, Behzad Jafari, Kazem D. Safa, Siavoush DastmalchiAbstractModified Fe3O4 magnetic nanoparticles (magnetic nanocarrier) technology have found the proper place in separation and purification techniques, such as protein and enzyme purification, mostly due to its easy and fast operational procedure by using an external permanent magnet. Herein, Fe3O4 magnetic nanoparticles were prepared, and surface modification was performed with vanadate and phosphate salts to yield four various model of magnetic nanocarriers...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Baicalin and berberine ultradeformable vesicles as potential adjuvant in vitiligo therapy
In conclusion, baicalin or berberine ultradeformable vesicles, and particularly their combination, may represent promising nanosystem-based adjuvants for the treatment of vitiligo symptoms.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Cell-assisted synthesis of conducting polymer – polypyrrole – for the improvement of electric charge transfer through fungal cell wall
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Roxana-Mihaela Apetrei, Geta Carac, Almira Ramanaviciene, Gabriela Bahrim, Catalin Tanase, Arunas RamanaviciusAbstractIn this research we report the biological synthesis of electrically conducting polymer – Polypyrrole (Ppy). Cell-assisted enzymatic polymerization/oligomerization of Ppy was achieved using whole cell culture and cell-free crude enzyme extract from two white-rot fungal cultures. The selected fungal strains belong to Trametes spp., known laccase producers, commonly applied in bioremediation and bioelectroc...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

The innovative fabrication and applications of carvacrol nanoemulsions, carboxymethyl chitosan microgels and their composite films
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Kai Lei, Xinran Wang, Xiaozhou Li, Lin WangAbstractCarvacrol (CA) was firstly made into physically stable nanoemulsions using an innovative spontaneous emulsification method with carvacrol itself as oil phase without introducing other carrier oils. Self-crosslinking carboxymethyl chitosan (CMC) microgels via intermolecular hydrogen bonds were then fabricated using a simple solvent exchange method with ethanol as poor solvent. The CMC microgels obtained were rapidly deposited on the surface of tin foil using an electrospray te...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

A rapid microwave-assisted phosphoric-acid treatment on carbon fiber surface for enhanced cell immobilization in xylitol fermentation
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Le Wang, Yanli Yin, Shuaibing Zhang, Dapeng Wu, Yangyong Lv, Yuansen Hu, Qixian Wei, Qipeng Yuan, Jinshui WangAbstractA rapid microwave-assisted phosphoric-acid treatment (MP) was applied to modify the surface of carbon fibers (CFs) for cell immobilization and fermentation. After the novel treatment, the surface of CFs subjected to MP (CF-MP) contained the CPO and COP bonds and the surface area and hydrophilicity were enhanced, which improved the cell adhesion and growth compared with the raw CF. Because of these structural a...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Synthesis and characterization of Barium–Iron containing magnetic bioactive glasses: The effect of magnetic component on structure and in vitro bioactivity
This study is focused on investigating effects of magnetic content addition on the bioactive glass properties. To this aim, we evaluate the physical, rheological, and biocompatibility properties of synthesized magnetic bioactive glass. The morphology and composition of these glasses were studied using X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The particle size was also determined using Laser Particle Size Analyzer (LPSA). The thermal measurements were carried out using Differential Thermal Analysis (DTA). For assessing the in-vitro bioactive character of synthe...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Cell membrane mimetic copolymer coated polydopamine nanoparticles for combined pH-sensitive drug release and near-infrared photothermal therapeutic
In this study, we modified the well-known photothermal polydopamine nanoparticles with the poly[(2-methacryloyloxy)ethyl phosphorylcholine-b-(2-dimethylamino)ethyl methacrylate] (PMPC-b-PDMAEMA) diblock copolymers, containing both biocompatible cell membrane phosphorylcholine zwitterions segments and pH-responsive dimethylaminoethyl units on the polymer chains, to achieve both the photothermal property and pH-responsive release behavior. The results showed that the obtained nanoparticles had a narrow size distribution with the diameter about 220 nm. Besides, the modified polydopamine nanoparticles showed enhanced colloidal...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Encapsulation of monomyristin into polymeric nanoparticles improved its in vitro antiproliferative activity against cervical cancer cells
Publication date: 1 April 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 176Author(s): Sirirat Boondireke, Michèle Léonard, Alain Durand, Benjamas Thanomsub WongsatayanonAbstractThe cytotoxicity of monomyristin (MM), a monoacylglycerol, was investigated against cervical cancer cells (HeLa) and two normal cells (Vero and endometrial epithelial cells). MM exhibited cytotoxicity specifically to HeLa cells and not against normal cells except at the highest investigated doses (> 500 μg/mL). MM was showed to increase apoptotic dead cells by intrinsic mitochondrial pathway. To overcome the p...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Peptide-functionalized supported lipid bilayers to construct cell membrane mimicking interfaces
In this study, RGD and Osteocalcin mimetic (OSN) peptides were used as model molecules for functionalization of otherwise passive SLBs to evaluate cell-surface interactions via real-time monitoring in quartz crystal microbalance with dissipation. Similar platforms were also used in cell culture environment. It was seen that low density of mobile RGD peptides on SLB platforms preserved their biological activity and promoted cell adhesion more efficiently than high number of immobile, physisorbed peptides. Even though nonspecific protein and cell attachment was promoted, cells did not spread well on OSN-coated control surfac...
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Strontium/adiponectin co-decoration modulates the osteogenic activity of nano-morphologic polyetheretherketone implant
This study provides bioactive Sr and APN as promising active components for bio-functional bone regeneration/substitution, and optimizes the osteointegration of PEEK implants in clinic.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - December 25, 2018 Category: Biochemistry Source Type: research

Molybdenum Disulfide Nanosheet Decorated with Silver Nanoparticles for Selective Detection of Dopamine
Publication date: Available online 21 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): M. Sookhakian, W.J. Basirun, Boon Tong Goh, Pei Meng Woi, Y. AliasAbstractA metal-inorganic composite, comprises of silver-molybdenum disulfide nanosheets (Ag@MoS2) was synthesized at low temperature. The Ag@MoS2 composite was drop-casted onto a glassy carbon electrode (GCE) for a highly selective dopamine (DA) detection in the presence of interfering compounds such as uric acid (UA) and ascorbic acid (AA). The physicochemical analysis of the nanosheets was carried out with X-ray diffraction, transmission electron mi...
Source: Colloids and Surfaces B: Biointerfaces - December 24, 2018 Category: Biochemistry Source Type: research

Characterization of M1 and M2 polarization phenotypes in peritoneal macrophages after treatment with graphene oxide nanosheets
Publication date: Available online 22 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): María José Feito, Rosalía Diez-Orejas, Mónica Cicuéndez, Laura Casarrubios, José María Rojo, María Teresa PortolésAbstractMacrophages play a key role in nanoparticle removal and are primarily responsible for their uptake and trafficking in vivo. Due to their functional plasticity, macrophages display a spectrum of phenotypes between two extremes indentified as pro-inflammatory M1 and reparative M2 macrophages, characterized by the expression of specific ...
Source: Colloids and Surfaces B: Biointerfaces - December 24, 2018 Category: Biochemistry Source Type: research

Regulation of osteoblast differentiation by osteocytes cultured on sclerostin antibody conjugated TiO2 nanotube array
This study provides a simple strategy to engineer titanium surface for bone fracture recovery, especially in osteoporotic conditions.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - December 24, 2018 Category: Biochemistry Source Type: research

Type I collagen peptides and nitric oxide releasing electrospun silk fibroin scaffold: A multifunctional approach for the treatment of ischemic chronic wounds
This study investigates a strategy of combining a regenerative component, Type I collagen Peptide (CP), along with a nitric oxide donor, S-Nitrosoglutathione (GSNO), in the form of nanofibrous scaffold to address the non-healing diabetic ulcer. Silk Fibroin-Polyvinyl alcohol (SF-PVA) nanofibrous scaffold is used as a carrier for delivering functional moieties. The developed nanofibrous electrospun mats (SF-PVA, CP-SF-PVA, and CP-GSNO-SF-PVA) showed continuous, bead-less and randomly oriented fibers with highly porous morphology. The in vitro biocompatibility was assessed by MTT assay, DAPI-Rhodamine 123 and FITC-Phalloidin...
Source: Colloids and Surfaces B: Biointerfaces - December 22, 2018 Category: Biochemistry Source Type: research

Carrier particle mediated stabilization and isolation of valsartan nanoparticles
In this study nanoparticles of the API valsartan were generated via a reverse antisolvent process at high supersaturations. Montmorillonite (MMT) and protamine functionalized montmorillonite (PA-MMT) were employed for stabilization and isolation of the valsartan (Val) nanoparticles (ca. 50 nm) into a dried form. A high dissolution rate of the resultant solid formulation at high drug loadings (up to 33.3% w/w) was achieved. The dissolution rates of the isolated valsartan nanoparticle carrier composites (dried Val-MMT nanocomposites and dried Val-PA-MMT nanocomposites) were similar to that of freshly prepared suspende...
Source: Colloids and Surfaces B: Biointerfaces - December 20, 2018 Category: Biochemistry Source Type: research

Immobilization of type I collagen/hyaluronic acid multilayer coating on enoxacin loaded titania nanotubes for improved osteogenesis and osseointegration in ovariectomized rats
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Hui Li, Bin’en Nie, Shutao Zhang, Teng Long, Bing YueAbstractTitania nanotubes (Ti-NTs) have been proven to be good drug carriers and can release drugs efficiently around implants. Enoxacin (EN) is a broad-spectrum antibiotic that has the ability of anti-osteoclastogenesis. Immobilization of extracellular matrix components on the surface of the material can greatly enhance the biological activity of the implant and slow down the release rate of the drug in Ti-NTs. In the present study, a material system that provided un...
Source: Colloids and Surfaces B: Biointerfaces - December 20, 2018 Category: Biochemistry Source Type: research

Photodegradable coumarin-derived amphiphilic dendrons for DNA binding: Self-assembly and phototriggered disassembly in water and air-water interface
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Jia-Yu Ou, Yu-Chan Shih, Bing-Yen Wang, Chih-Chien ChuAbstractIn this article, we demonstrate the self-assembly and photoresponive behavior of a novel coumarin-based amphiphilic dendron in both aqueous solution and air-water interface. The dendritic structure, namely C-IG1, was composed of a lipophilic cholesterol and hydrophilic poly(amido amine) (PAMAM) dendron, and the amphiphilic counterpart is interconnected by a photolabile coumarin carbonate ester, enabling the photoinduced degradation of the amphiphiles in protic solv...
Source: Colloids and Surfaces B: Biointerfaces - December 20, 2018 Category: Biochemistry Source Type: research

Synthesis and fabrication of a keratin-conjugated insulin hydrogel for the enhancement of wound healing
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Wenfeng Li, Feiyan Gao, Jinlan Kan, Jia Deng, Bochu Wang, Shilei HaoAbstractAccelerating and regulating collagen formation during wound healing repair is key issues for skin regeneration. Insulin can promote the healing of damaged skin by stimulating cellular migration and angiogenesis. Here, human hair keratin-conjugated insulin was synthesized to enhance full-thickness skin regeneration based on the excellent wound healing and hemostatic effects of keratin and the collagen deposition regulation ability of insulin. The insul...
Source: Colloids and Surfaces B: Biointerfaces - December 20, 2018 Category: Biochemistry Source Type: research

PEG-coated vesicles from Pluronic/lipid mixtures for the carrying of photoactive erythrosine derivatives
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Camila Fabiano de Freitas, Italo Rodrigo Calori, Ana Claudia Pedrozo da Silva, Lidiane Vizioli de Castro, Francielle Sato, Diogo Silva Pellosi, André Luiz Tessaro, Wilker Caetano, Noboru HiokaAbstractLiposomes are very attractive membrane models and excellent drug delivery systems. Concerning their drug delivery aspects, the mixing liposomes with biocompatible copolymers allows for stability and the incorporation of several drugs. We developed PEG coated vesicles from the mixture of DPPC and F127 Pluronic copolymer to ...
Source: Colloids and Surfaces B: Biointerfaces - December 20, 2018 Category: Biochemistry Source Type: research

Defined cell adhesion for silicon-based implant materials by using vapor-deposited functional coatings
Publication date: 1 March 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 175Author(s): Chih-Yu Wu, Zhen-Yu Guan, Pin-Chen Lin, Shih-Ting Chen, Po-Kang Lin, Po-Chun Chen, Pen-Hsiu Grace Chao, Hsien-Yeh ChenAbstractThe field of implantable electronics relies on using silicon materials due to the merits of a well-established fabrication process and favorable properties; of particular interest is the surface modification of such materials. In the present study, we introduce a surface modification technique based on coatings of functionalized Parylene on silicon substrates, where the modified layers provide a define...
Source: Colloids and Surfaces B: Biointerfaces - December 20, 2018 Category: Biochemistry Source Type: research

Synergetic Effect of Vancomycin Loaded Silver Nanoparticles for Enhanced Antibacterial Activity
Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Amritpal Kaur, Simran Preet, Vivek Kumar, Rajeev Kumar, Rajesh KumarAbstractIn recent years, there is significant growth in the bacterial resistance to various classic antibiotics. This has opened and enhanced the field of metal nanoparticles and antibiotic-metal nanoparticle complex. This research was designed to load a glycopeptide antibiotic named vancomycin on citrate-capped silver nanoparticles to enhance its antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia...
Source: Colloids and Surfaces B: Biointerfaces - December 19, 2018 Category: Biochemistry Source Type: research

Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles
Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Ekaterina Chudinova, Maria Surmeneva, Alexander S. Timin, Timofey E. Karpov, Alexandra Wittmar, Mathias Ulbricht, Anna Ivanova, Kateryna Loza, Oleg Prymak, Andrey Koptyug, Matthias Epple, Roman A. SurmenevAbstractIn the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposit...
Source: Colloids and Surfaces B: Biointerfaces - December 19, 2018 Category: Biochemistry Source Type: research

Direct stimulation of cellular immune response via TLR2 signaling triggered by contact with hybrid glyco-biointerfaces composed of chitohexaose and cellohexaose
In this report, hybrid self-assembled monolayers (SAMs) of chitohexaose (βGlcNAc6) and cellohexaose (βGlc6) are prepared to induce a highly sensitive, glyco-density-dependent inflammatory response by HEK293 cells expressing toll-like receptor 2 (TLR2), which recognizes oligo-βGlcNAc moieties. Thiosemicarbazide-labeled βGlcNAc6 and βGlc6, as a bio-signal inducer and a spacer molecule, respectively, were immobilized on a gold substrate via spontaneous chemisorption to control the βGlcNAc6 density on the SAM surface. The βGlcNAc6 density ranged from 0 to 0.65 chains nm–2. The inflammat...
Source: Colloids and Surfaces B: Biointerfaces - December 19, 2018 Category: Biochemistry Source Type: research

Redox-dependent catalase mimetic cerium oxide-based nanozyme protect human hepatic cells from 3-AT induced acatalasemia
In this study, we have artificially irreversibly inhibited the activity of cellular catalase enzyme from human liver cells (WRL-68) using 3-Amino-1,2,4-Triazole (3-AT). Further, CeNPs was used for imparting protective effect against the deleterious effects of elevated cellular H2O2 concentration. Our results suggest that CeNPs (+4) can protect hepatic cells from cytotoxicity and genetic damage from the high concentrations of H2O2 in the absence of functional catalase enzyme. CeNPs were efficiently internalized in WRL-68 cells and effectively scavenge the free radicals generated due to elevated H2O2 inside the cells. Additi...
Source: Colloids and Surfaces B: Biointerfaces - December 18, 2018 Category: Biochemistry Source Type: research

The contribution of hydrogen peroxide to the radiosensitizing effect of gold nanoparticles
Publication date: Available online 17 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Talat Tariq Khalil, Rana Bazzi, Stéphane Roux, Michel FrommAbstractPlasmid DNA in aerated aqueous solution is used as a probe to determine whose of the reactive oxygen species (ROS) generated after absorption of ultra-soft X-rays (USX) take part in biomolecule damage in the presence and in absence of Gold Nano-Particles (GNP) and specific scavengers. Citrate-coated GNPs with core sizes of 6, 10 and 25 nm are synthetized and characterized, especially in terms of plasmon band shift, ζ-potential and h...
Source: Colloids and Surfaces B: Biointerfaces - December 18, 2018 Category: Biochemistry Source Type: research

Functionalized Polystyrene Microspheres as Cryptosporidium Surrogates
Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Lu Liu, Yinan Wang, Ravin Narain, Yang LiuGraphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - December 18, 2018 Category: Biochemistry Source Type: research

The enhancement of surface activity and nanoparticle stability through the alteration of charged amino acids of HGFI
Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jiuxia Yang, Bo Wang, Lu Ge, Xiaotian Yang, Xiangxiang Wang, Yixin Dai, Baolong Niu, Haijin Xu, Mingqiang QiaoAbstractHGFI (hydrophobin found in Grifola frondosa ) is a small surface-active protein, belonging to class I hydrophobins, and can form amphipathic protein films at hydrophobic- hydrophilic material interfaces. HGFI has a low isoelectric point (pI) because no basic amino acids are present in its sequence. Here we constructed two HGFI mutants using a site-directed mutation method. The two mutants were named M...
Source: Colloids and Surfaces B: Biointerfaces - December 18, 2018 Category: Biochemistry Source Type: research