A role for host cell exocytosis in InlB ‐mediated internalization of Listeria monocytogenes
SUMMARY
The bacterial surface protein InlB mediates internalization of Listeria monocytogenes into human cells through interaction with the host receptor tyrosine kinase, Met. InlB‐mediated entry requires localized polymerization of the host actin cytoskeleton. Apart from actin polymerization, roles for other host processes in Listeria entry are unknown. Here we demonstrate that exocytosis in the human cell promotes InlB‐dependent internalization. Using a probe consisting of VAMP3 with an exofacial Green Fluorescent Protein (GFP) tag, focal exocytosis was detected during InlB‐mediated entry. Exocytosis was dependent ...
Source: Cellular Microbiology - July 26, 2017 Category: Microbiology Authors: Hoan Van Ngo, Manmeet Bhalla, Da ‐Yuan Chen, Keith Ireton Tags: RESEARCH ARTICLE Source Type: research
Listeria monocytogenes cytosolic metabolism promotes replication, survival, and evasion of innate immunity
Summary
Listeria monocytogenes, the causative agent of listeriosis, is an intracellular pathogen that is exquisitely evolved to survive and replicate in the cytosol of eukaryotic cells. Eukaryotic cells typically restrict bacteria from colonising the cytosol, likely through a combination of cell autonomous defences, nutritional immunity, and innate immune responses including induction of programmed cell death. This suggests that L. monocytogenes and other professional cytosolic pathogens possess unique metabolic adaptations, not only to support replication but also to facilitate resistance to host‐derived stresses/defenc...
Source: Cellular Microbiology - July 21, 2017 Category: Microbiology Authors: Grischa Y. Chen, Daniel A. Pensinger, John ‐Demian Sauer Tags: MICROREVIEW Source Type: research
Genome packaging of reovirus is mediated by the scaffolding property of the microtubule network
Summary
Reovirus replication occurs in the cytoplasm of the host cell, in virally induced mini‐organelles called virus factories. On the basis of the serotype of the virus, the virus factories can manifest as filamentous (type 1 Lang strain) or globular structures (type 3 Dearing strain). The filamentous factories morphology is dependent on the microtubule cytoskeleton; however, the exact function of the microtubule network in virus replication remains unknown. Using a combination of fluorescent microscopy, electron microscopy, and tomography of high‐pressure frozen and freeze‐substituted cells, we determined the ult...
Source: Cellular Microbiology - July 21, 2017 Category: Microbiology Authors: Pranav N.M. Shah, Megan L. Stanifer, Katharina H öhn, Ulrike Engel, Uta Haselmann, Ralf Bartenschlager, Hans‐Georg Kräusslich, Jacomine Krijnse‐Locker, Steeve Boulant Tags: RESEARCH ARTICLE Source Type: research
Retroviruses and microtubule ‐associated motor proteins
Summary
Retroviruses are obligate intracellular parasites of eukaryotic cells. After reverse transcription, the viral DNA contained in the preintegration complex is delivered to the nucleus of the host cell, where it integrates. Before reaching the nucleus, the incoming particle and the preintegration complex must travel throughout the cytoplasm. Likewise, the newly synthesized viral proteins and viral particles must transit the cytoplasm during exit. The cytoplasm is a crowded environment, and simple diffusion is difficult. Therefore, viruses have evolved to utilize the cellular mechanisms of movement through the cytoplas...
Source: Cellular Microbiology - July 12, 2017 Category: Microbiology Authors: Gloria Arriagada Tags: MICROREVIEW Source Type: research
Issue Information
No abstract is available for this article. (Source: Cellular Microbiology)
Source: Cellular Microbiology - July 10, 2017 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
