Quantitative measurement of HER2 expression in breast cancers: comparison with ‘real-world’ routine HER2 testing in a multicenter Collaborative Biomarker Study and correlation with overall survival
In this study, we compared quantitative HER2 expression by the HERmark™ Breast Cancer Assay (HERmark) with routine HER2 testing by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and correlated HER2 results with overall survival (OS) of breast cancer patients in a multicenter Collaborative Biomarker Study (CBS).
Methods:
Two hundred and thirty-two formalin-fixed, paraffin-embedded breast cancer tissues and local laboratory HER2 testing results were provided by 11 CBS sites. HERmark assay and central laboratory HER2 IHC retesting were retrospectively performed in a blinded fashion. HER2 results b...
Source: Breast Cancer Research - March 18, 2015 Category: Cancer & Oncology Authors: Denise YardleyPeter KaufmanWeidong HuangLea KrekowMichael SavinWilliam LawlerStephen ZradaAlexander StarrHarvey EinhornLee SchwartzbergJohn AdamsYolanda LieAgnes PaquetJeff SperindeMojgan HaddadSteve AndersonMarlon BriginoRick PesanoMichael BatesJodi Weid Source Type: research
Quantitative measurement of HER2 expression in breast cancers: comparison with “real world” routine HER2 testing in a multi-center Collaborative Biomarker Study and correlation with overall survival
In this study, we compared quantitative HER2 expression by the HERmark® Breast Cancer Assay (HERmark) with routine HER2 testing by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and correlated HER2 results with overall survival (OS) of breast cancer patients in a multi-center Collaborative Biomarker Study (CBS).
Methods:
232 formalin-fixed, paraffin-embedded breast cancer tissues and local laboratory HER2 testing results were provided by 11 CBS sites. HERmark assay and central laboratory HER2 IHC re-testing were retrospectively performed in a blinded fashion. HER2 results by all testing methods ...
Source: Breast Cancer Research - March 18, 2015 Category: Cancer & Oncology Authors: Denise YardleyPeter KaufmanWeidong HuangLea KrekowMichael SavinWilliam LawlerStephen ZradaAlexander StarrHarvey EinhornLee SchwartzbergJohn AdamsYolanda LieAgnes PaquetJeff SperindeMojgan HaddadSteve AndersonMarlon BriginoRick PesanoMichael BatesJodi Weid Source Type: research
Mechanisms of endocrine resistance in breast cancer: an overview of the proposed roles of noncoding RNA
Endocrine therapies such as tamoxifen and aromatase inhibitors are the standard treatment options for estrogen receptor-positive breast cancer patients. However, resistance to these agents has become a major clinical obstacle. Potential mechanisms of resistance to endocrine therapies have been identified, often involving enhanced growth factor signaling and changes in the expression or action of the estrogen receptor, but few studies have addressed the role of noncoding RNA (ncRNA). Two important types of ncRNA include microRNA (miRNA) and long noncoding RNA (lncRNA). miRNAs are small RNA molecules that regulate gene expre...
Source: Breast Cancer Research - March 17, 2015 Category: Cancer & Oncology Authors: Erin HayesJoan Lewis-Wambi Source Type: research
Estimated dietary dioxin exposure and breast cancer risk among women from the French E3N prospective cohort
Conclusions:
Overall, no association between estimated dietary dioxin exposure and BC risk was found among E3N women. Further studies should include both dietary and environmental exposures to determine whether low-dose dioxin exposure is associated with BC risk. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 17, 2015 Category: Cancer & Oncology Authors: Aurélie DanjouBéatrice FerversMarie-Christine Boutron-RuaultThierry PhilipFrançoise Clavel-ChapelonLaure Dossus Source Type: research
Pleiotropic functions of the tumor- and metastasis-suppressing Matrix Metalloproteinase-8 in mammary cancer in MMTV-PyMT transgenic mice
Conclusions:
These findings provide novel insight into the suppressive action of MMP-8 on mammary tumorigenesis and metastasis, and indicate that the loss of MMP-8 likely has pleiotropic effects on innate immunity and angiogenesis that are reflected in changes in the protease web. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 14, 2015 Category: Cancer & Oncology Authors: Julie DecockWouter HendrickxSally ThirkettleAna Gutiérrez-FernándezStephen RobinsonDylan Edwards Source Type: research
Lobular breast cancer: incidence and genetic and non-genetic risk factors
While most invasive breast cancers consist of carcinomas of the ductal type, about 10% are invasive lobular carcinomas. Invasive lobular and ductal carcinomas differ with respect to risk factors. Invasive lobular carcinoma is more strongly associated with exposure to female hormones, and therefore its incidence is more subject to variation. This is illustrated by US figures during the 1987 to 2004 period: after 12 years of increases, breast cancer incidence declined steadily from 1999 to 2004, reflecting among other causes the decreasing use of menopausal hormone therapy, and these variations were stronger for invasive lo...
Source: Breast Cancer Research - March 13, 2015 Category: Cancer & Oncology Authors: Laure DossusPatrick Benusiglio Source Type: research
Integrative analyses identify modulators of response to neoadjuvant aromatase inhibitors in patients with early breast cancer
Conclusions:
These data provide a rationale for investigation of the role of CHKA in further models of de novo and acquired resistance to AIs, and provide proof of concept that integrative genomic analyses can identify biologically relevant modulators of AI response. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 11, 2015 Category: Cancer & Oncology Authors: Elena López-KnowlesPaul WilkersonRicardo RibasHelen AndersonAlan MackayZara GhazouiAradhana RaniPeter OsinAsh NerurkarLorna RenshawAlexey LarionovWilliam MillerJ DixonJorge Reis-FilhoAnita DunbierLesley-Ann MartinMitch Dowsett Source Type: research
Lectin RCA-I specifically binds to metastasis-associated cell surface glycans in triple negative breast cancer
Conclusions:
We discovered RCA-I, that bound to TNBC cells to a degree that is proportional to their metastatic capacities, found that this binding inhibits the cell invasion, migration, and adhesion, and identified a membrane protein, POTEF, that may play a key role in mediating these effects. These results thus indicate that RCA-I specific cell surface glycoproteins may play a critical role in TNBC metastasis and that the extent of RCA-I cell binding could be used in diagnosis to predict the likelihood of developing metastases in TNBC patients. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 11, 2015 Category: Cancer & Oncology Authors: Shu-Min ZhouLi ChengShu-Juan GuoYang WangDaniel CzajkowskyHuafang GaoXiao-Fang HuSheng-Ce Tao Source Type: research
Presence of circulating Her-2-reactive CD8 + T-cells is associated with lower frequencies of myeloid-derived suppressor cells and regulatory T cells, and better survival in elderly breast cancer patients
Conclusions:
Our data demonstrate a negative role of both MDSCs and Tregs in the prognosis of breast cancer patients, the mechanism of which might be through dampening favorable CD8+ T cell immune responses to tumour-associated antigens. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 10, 2015 Category: Cancer & Oncology Authors: Jithendra BailurBrigitte GueckelEvelyna DerhovanessianGraham Pawelec Source Type: research
Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), enhances anti-tumor effects of the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib in triple-negative breast cancer cells
Conclusion:
Our findings suggest that expression of functional PTEN may serve as a biomarker for selecting TNBC patients that would favorably respond to a combination of olaparib with SAHA. This provides a strong rationale for treating TNBC patients with PTEN expression with a combination therapy consisting of olaparib and SAHA. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 7, 2015 Category: Cancer & Oncology Authors: Ahrum MinSeock-Ah ImDebora KimSang-Hyun SongHee-Jun KimKyung-Hun LeeTae-Yong KimSae-Won HanDo-Youn OhTae-You KimMark O¿ConnorYung-Jue Bang Source Type: research
Mouse mammary stem cells express prognostic markers for triple-negative breast cancer
Conclusions:
Our findings support the hypothesis that the biological properties of normal stem cells are drivers of metastasis and that these properties can be used to stratify patients with a highly heterogeneous disease such as TNBC. (Source: Breast Cancer Research)
Source: Breast Cancer Research - March 4, 2015 Category: Cancer & Oncology Authors: Kelly SoadyHoward KendrickQiong GaoAndrew TuttMarketa ZvelebilLiliana OrdonezJelmar QuistDavid TanClare IsackeAnita GrigoriadisMatthew Smalley Source Type: research
Changes in insulin receptor signaling underlie neoadjuvant metformin administration in breast cancer: a prospective window of opportunity neoadjuvant study
IntroductionThe anti-diabetic drug metformin exhibits potential anti-cancer properties, which are believed to involve both direct (insulin-independent) and indirect (insulin-dependent) actions. Direct effects are linked to activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and an inhibition of mammalian target of rapamycin (mTOR) signaling, while indirect effects are mediated by reductions in circulating insulin, leading to reduced insulin receptor (IR)-mediated signaling. However, the in vivo impact of metformin on cancer cell signaling, and the factors governing sensitivity in patients remain unk...
Source: Breast Cancer Research - March 3, 2015 Category: Cancer & Oncology Authors: Ryan DowlingSaroj NiraulaMartin ChangSusan DoneMarguerite EnnisDavid McCreadyWey LeongJaime EscallonMichael ReedijkPamela GoodwinVuk Stambolic Source Type: research
Molecular subtyping for clinically defined breast cancer subgroups
IntroductionBreast cancer is commonly classified into intrinsic molecular subtypes. Standard gene centering is a routinely done prior to molecular subtyping but can produce inaccurate classifications when the distribution of clinicopathological characteristics in the study cohort differs from that of the training cohort which was used to derive the classifier.
Methods:
We propose a subgroup-specific gene centering method to perform molecular subtyping on a study cohort that has a skewed distribution of clinicopathological characteristics relative to the training cohort. On such a study cohort, we center each gene on a spec...
Source: Breast Cancer Research - February 26, 2015 Category: Cancer & Oncology Authors: Xi ZhaoEinar RødlandRobert TibshiraniSylvia Plevritis Source Type: research
FOXF2 deficiency promotes epithelial-mesenchymal transition and metastasis of basal-like breast cancer
Conclusions:
FOXF2 is a novel EMT-suppressing transcription factor in BLBC. FOXF2 deficiency enhances metastatic ability of BLBC cells by activating the EMT program through upregulating the transcription of TWIST1. (Source: Breast Cancer Research)
Source: Breast Cancer Research - February 26, 2015 Category: Cancer & Oncology Authors: Qing-Shan WangPeng-Zhou KongXiao-Qing LiFan YangYu-Mei Feng Source Type: research
Combined histone deacetylase inhibition and tamoxifen induces apoptosis in tamoxifen resistant breast cancer models, by reversing Bcl-2 overexpression
Conclusion:
Our model implicates elevated ER and Bcl-2 as key drivers of anti-estrogen resistance, which can be reversed by epigenetic modulation through HDAC inhibition. (Source: Breast Cancer Research)
Source: Breast Cancer Research - February 25, 2015 Category: Cancer & Oncology Authors: Paromita RahaScott ThomasK ThurnJeenah ParkPamela Munster Source Type: research
