Biochimica et Biophysica Acta (BBA) Proteins and Proteomics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Changes in strand 6B and helix B during neuroserpin inhibition: Implication in severity of clinical phenotype
Publication date: Available online 16 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Mohammad Farhan Ali, Abhinav Kaushik, Dinesh Gupta, Shoyab Ansari, Mohamad Aman JairajpuriAbstractNeuroserpin (NS) is predominantly expressed in brain and inhibits tissue-type plasminogen activator (tPA) with implications in brain development and memory. Nature of conformational change in pathological variants in strand 6B and helix B of NS that cause a relatively mild to severe epilepsy (and/or dementia) remains largely elusive. MD simulation with wild type (WT) NS, strand 6B and helix B varian...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 17, 2020 Category: Biochemistry Source Type: research

The alpha helix of the intermediate region in hGBP-1 acts as a coupler for enhanced GMP formation
Publication date: Available online 16 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Sudeepa Rajan, Apurba Kumar SauAbstractThe interferon-gamma inducible large GTPase human guanylate binding protein-1 (hGBP-1) plays a key role in anti-pathogenic and anti-proliferative functions. This protein hydrolyzes GTP to both GDP and GMP (predominant product) through sequential phosphate cleavages, which makes it functionally distinct from other GTPases. Previous study on truncated variants of hGBP-1 suggested that the α-helix present in the intermediate region is essential for dimerizati...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 17, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: March 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1868, Issue 3Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 17, 2020 Category: Biochemistry Source Type: research

Falcipain cysteine proteases of malaria parasites: An update
Publication date: Available online 9 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Philip J. RosenthalAbstractBackgroundThe malaria parasite Plasmodium falciparum expresses four related papain-family cysteine proteases known as falcipains. These proteases play critical roles in the parasite life cycle, and as such are potential targets for new modes of antimalarial chemotherapy, as discussed in this review.Scope of reviewThis review summarizes available knowledge describing falcipain cysteine proteases of malaria parasites.Major conclusionsBased on available data the falcipains...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 11, 2020 Category: Biochemistry Source Type: research

Cysteinyl cathepsins in cardiovascular diseases
Publication date: Available online 9 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Xian Zhang, Songyuan Luo, Minjie Wang, Guo-Ping ShiAbstractCysteinyl cathepsins are lysosomal/endosomal proteases that mediate bulk protein degradation in these intracellular acidic compartments. Yet, studies indicate that these proteases also appear in the nucleus, nuclear membrane, cytosol, plasma membrane, and extracellular space. Patients with cardiovascular diseases (CVD) show increased levels of cathepsins in the heart, aorta, and plasma. Plasma cathepsins often serve as biomarkers or risk ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 9, 2020 Category: Biochemistry Source Type: research

High-resolution structure of intramolecularly proteolyzed human mucin-1 SEA domain
We report the first high-resolution X-ray structure of cleaved MUC1 SEA. Structural comparison with uncleaved SEA domains suggests that the number of residues evolutionarily inserted in the cleaved loop of MUC1 SEA precludes the formation of a properly hydrogen-bonded beta turn. By sequence analysis, we show that this conformational frustration is shared by all known cleaved SEA domains. In addition, alternative conformations of the uncleaved precursor could be modeled in which the scissile peptide bond is planar. The implications of these structures for autoproteolysis are discussed in the light of the previous research o...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 8, 2020 Category: Biochemistry Source Type: research

Selective pressure on an interfacial enzyme: Functional roles of a highly conserved asparagine residue in a cellulase
Publication date: Available online 3 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Trine Holst Sørensen, Silke Flindt Badino, Michael Skovbo Windahl, Nanna Røjel, Brett McBrayer, Kim Borch, Peter Westh (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - January 5, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: February 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1868, Issue 2Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 24, 2019 Category: Biochemistry Source Type: research

Analysis of uracil DNA glycosylase (UNG2) stimulation by replication protein A (RPA) at ssDNA-dsDNA junctions
Publication date: Available online 19 December 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Brian P. WeiserAbstractReplication Protein A (RPA) is a single-stranded DNA binding protein that interacts with DNA repair proteins including Uracil DNA Glycosylase (UNG2). Here, I report DNA binding and activity assays using purified recombinant RPA and UNG2. Using synthetic DNA substrates, RPA was found to promote UNG2's interaction with ssDNA-dsDNA junctions regardless of the DNA strand polarity surrounding the junction. RPA stimulated UNG2's removal of uracil bases paired with adenine or gu...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 20, 2019 Category: Biochemistry Source Type: research

Regulation of Herbaspirillum seropedicae NifA by the GlnK PII signal transduction protein is mediated by effectors binding to allosteric sites
Publication date: Available online 19 December 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Adriano Alves Stefanello, Marco Aurélio Schuler de Oliveira, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Leda Satie Chubatsu, Luciano Fernandes Huergo, Ray Dixon, Rose Adele MonteiroAbstractHerbaspirillum seropedicae is a plant growth promoting bacterium that is able to fix nitrogen and to colonize the surface and internal tissues of important crops. Nitrogen fixation in H. seropedicae is regulated at the transcriptional level by the prokaryotic enhancer binding protein NifA. The ac...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 20, 2019 Category: Biochemistry Source Type: research

Recent advances in biocatalysis and biotransformations
Publication date: Available online 18 December 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 18, 2019 Category: Biochemistry Source Type: research

Twenty-five years of nomenclature and classification of proteolytic enzymes
Publication date: Available online 12 December 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Neil D. RawlingsAbstractProteolytic enzymes and their homologues have been classified into clans by comparing the tertiary structures of the peptidase domains, into families by comparing the protein sequences of the peptidase domains, and into protein-species by comparing various attributes including domain architecture, substrate preference, inhibitor interactions, subcellular location, and phylogeny. The results are compared with the earlier classification by Rawlings & Barrett (Rawlings and ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 13, 2019 Category: Biochemistry Source Type: research

The structure of a prokaryotic feruloyl-CoA hydratase-lyase from a lignin-degrading consortium with high oligomerization stability under extreme pHs
In this study, a prokaryotic FCHL derived from a lignin-degrading microbial consortium (named LM-FCHL) was cloned, successfully expressed in soluble form and purified. The crystal structure was solved and refined at 2.1 Å resolution. The LM-FCHL is a hexamer composed of a dimer of trimers, which showed to be quite stable under extreme pH conditions. Finally, small angle X-ray scattering corroborates the hexameric state in solution and indicates flexibility in the protein structure. The present study contributes to the field of lignin valorization to valuable molecules by establishing the biophysical and structural chara...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 11, 2019 Category: Biochemistry Source Type: research

Small protease inhibitors in tick saliva and salivary glands and their role in tick-host-pathogen interactions
Publication date: Available online 7 December 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Larissa Almeida Martins, Jan Kotál, Chaima Bensaoud, Jindřich Chmelař, Michail KotsyfakisAbstractTicks must durably suppress vertebrate host responses (hemostasis, inflammation, immunity) to avoid rejection and act as vectors of many pathogenic microorganisms that cause disease in humans and animals. Transcriptomics and proteomics studies have been used to study tick-host-pathogen interactions and have facilitated the systematic characterization of salivary composition and molecular dynamics ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - December 7, 2019 Category: Biochemistry Source Type: research

Sterilization of epidermal growth factor with supercritical carbon dioxide and peracetic acid; analysis of changes at the amino acid and protein level
Publication date: Available online 29 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): David M. Bednarski, Ellen E. Lantz, Cedric E. Bobst, Anthony R. Eisenhut, Stephen J. Eyles, Julien P. FeyAbstractAseptic processing and terminal sterilization become increasingly challenging as medical devices become more complex and include active biologics. Terminal sterilization is preferred for patient safety and production costs. We aimed to determine how sterilization using supercritical CO2 (scCO2) with low levels of peracetic acid (PAA) affects amino acids and human epidermal growth fac...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 29, 2019 Category: Biochemistry Source Type: research