miR-29c-3p promotes senescence of human mesenchymal stem cells by targeting CNOT6 through p53–p21 and p16–pRB pathways
In conclusion, our results demonstrate that miR-29c-3p promotes the senescence of MSCs by targeting CNOT6 through p53–p21 and p16–pRB pathways and highlight the contribution of post-transcriptional regulation to stem cell senescence. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 29, 2016 Category: Molecular Biology Source Type: research

Skp2 inhibits osteogenesis by promoting ubiquitin–proteasome degradation of Runx2
Publication date: April 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1863, Issue 4 Author(s): Gatha Thacker, Yogesh Kumar, Mohd. Parvez Khan, Nidhi Shukla, Isha Kapoor, Jitendra Kumar Kanaujiya, Savita Lochab, Shakil Ahmed, Sabyasachi Sanyal, Naibedya Chattopadhyay, Arun Kumar Trivedi Osteogenic transcription factor Runx2 is essential for osteoblast differentiation. The activity of Runx2 is tightly regulated at transcriptional as well as post-translational level. However, regulation of Runx2 stability by ubiquitin mediated proteasomal degradation by E3 ubiquitin ligases is ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 29, 2016 Category: Molecular Biology Source Type: research

Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signalling
Publication date: Available online 22 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Franziska Kopp, Sarah Kupsch, Andra B. Schromm The Lipopolysaccharide-binding protein (LBP) is critically involved in innate immune responses to Gram-negative infections. We show here that human peripheral blood-derived monocytes, but not lymphocytes, stain positive for endogenous LBP on the cell surface. Studies on human macrophages demonstrate LBP binding at normal serum concentrations of 1–10μg/ml. Binding was increased in a concentration-dependent manner by lipopolysaccharide ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

Ca2+ microdomains, NAADP and type 1 ryanodine receptor in cell activation
This article is part of a Special Issue entitled: Calcium and Cell Fate edited by Jacques Haiech, Claus Heizmann and Joachim Krebs Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

Tumor microenvironment in diffuse large B-cell lymphoma: Matrixmetalloproteinases activation is mediated by osteopontin overexpression
Publication date: March 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1863, Issue 3 Author(s): Grazia Malaponte, Sameh Hafsi, Jerry Polesel, Giancarlo Castellano, Paola Spessotto, Claudio Guarneri, Silvana Canevari, Santo S. Signorelli, James A. McCubrey, Massimo Libra (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

Exosome-mediated microenvironment dysregulation in leukemia
This article is part of a Special Issue entitled: Tumor Microenvironment Regulation of Cancer Cell Survival, Metastasis, Inflammation, and Immune Surveillance edited by Peter Ruvolo and Gregg L. Semenza. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

Advances in understanding the acute lymphoblastic leukemia bone marrow microenvironment: From biology to therapeutic targeting
This article is part of a Special Issue entitled: Tumor Microenvironment Regulation of Cancer Cell Survival, Metastasis,Inflammation, and Immune Surveillance edited by Peter Ruvolo and Gregg L. Semenza. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

Roles of NGAL and MMP-9 in the tumor microenvironment and sensitivity to targeted therapy
This article is part of a Special Issue entitled: Tumor Microenvironment Regulation of Cancer Cell Survival, Metastasis, Inflammation, and Immune Surveillance edited by Peter Ruvolo and Gregg L. Semenza. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

Galectin 3 as a guardian of the tumor microenvironment
This article is part of a Special Issue entitled: Tumor Microenvironment Regulation of Cancer Cell Survival, Metastasis, Inflammation, and Immune Surveillance edited by Peter Ruvolo and Gregg L. Semenza. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

The role of microenvironment and immunity in drug response in leukemia
This article is part of a Special Issue entitled: Tumor Microenvironment Regulation of Cancer Cell Survival, Metastasis, Inflammation, and Immune Surveillance edited by Peter Ruvolo and Gregg L. Semenza. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 25, 2016 Category: Molecular Biology Source Type: research

The neonate versus adult mammalian immune system in cardiac repair and regeneration
This article is part of a Special Issue entitled:Cardiomyocyte Biology: Integration of Develomental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 21, 2016 Category: Molecular Biology Source Type: research

TCTP regulates spindle microtubule dynamics by stabilizing polar microtubules during mouse oocyte meiosis
Publication date: Available online 21 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Hyuk-Joon Jeon, Seung Yeop You, Yong Seok Park, Jong Wook Chang, Jae-Sung Kim, Jeong Su Oh Dynamic changes in spindle structure and function are essential for maintaining genomic integrity during the cell cycle. Spindle dynamics are highly dependent on several microtubule-associated proteins that coordinate the dynamic behavior of microtubules, including microtubule assembly, stability and organization. Here, we show that translationally controlled tumor protein (TCTP) is a novel microt...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 21, 2016 Category: Molecular Biology Source Type: research

How does α-actinin-3 deficiency alter muscle function? Mechanistic insights into ACTN3, the ‘gene for speed’
Publication date: Available online 21 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Fiona X.Z. Lee, Peter J. Houweling, Kathryn N. North, Kate G.R. Quinlan An estimated 1.5 billion people worldwide are deficient in the skeletal muscle protein α-actinin-3 due to homozygosity for the common ACTN3 R577X polymorphism. α-Actinin-3 deficiency influences muscle performance in elite athletes and the general population. The sarcomeric α-actinins were originally characterised as scaffold proteins at the muscle Z-line. Through studying the Actn3 knockout mouse and...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 21, 2016 Category: Molecular Biology Source Type: research

Putting together the clues of the everlasting neuro-cardiac liaison
This article is part of a Special Issue entitled:Cardiomyocyte Biology: Integration of Develomental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 15, 2016 Category: Molecular Biology Source Type: research

Skp2 inhibits osteogensis by promoting ubiquitin-proteasome degradation of Runx2
Publication date: Available online 14 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Gatha Thacker, Yogesh Kumar, Mohd. Parvez Khan, Nidhi Shukla, Isha Kapoor, Jitendra Kumar Kanaujiya, Savita Lochab, Shakil Ahmed, Sabyasachi Sanyal, Naibedya Chattopadhyay, Arun Kumar Trivedi Osteogenic transcription factor Runx2 is essential for osteoblast differentiation. Activity of Runx2 is tightly regulated at transcriptional as well as post-translational level. However regulation of Runx2 stability by ubiquitin mediated proteasomal degradation by E3 ubiquitin ligases is littl...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 15, 2016 Category: Molecular Biology Source Type: research

The acetylenic tricyclic bis(cyano enone), TBE-31, targets microtubule dynamics and cell polarity in migrating cells
Publication date: Available online 13 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Eddie Chan, Akira Saito, Tadashi Honda, Gianni M. Di Guglielmo Cell migration is dependent on the microtubule network for structural support as well as for the proper delivery and positioning of polarity proteins at the leading edge of migrating cells. Identification of drugs that target cytoskeletal-dependent cell migration and protein transport in polarized migrating cells is important in the understanding the cell biology of normal and tumor cells and can lead to new therapeutic target...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 14, 2016 Category: Molecular Biology Source Type: research

A stromal interaction molecule1 variant up-regulates matrix metalloproteinase-2 expression by strengthening nucleoplasmic Ca2+ signaling
Publication date: Available online 13 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Fengrong Chen, Liping Zhu, Lei Cai, Jiwei Zhang, Xianqin Zeng, Jiansha Li, Yuan Su, Qinghua Hu Very recent studies hold promise to reveal the role of stromal interaction molecule 1 (STIM1) in non-store-operated Ca2+ entry. Here we showed that in contrast to cytoplasmic membrane redistribution as previously noted, human umbilical vein endothelial STIM1 with a T-to-C nucleotide transition resulting in an amino acid substitution of leucine by proline in the signal peptide sequence transl...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 14, 2016 Category: Molecular Biology Source Type: research

Histone demethylase Jmjd3 regulates osteoblast apoptosis through targeting anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bim
Publication date: Available online 12 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Di Yang, Hirohiko Okamura, Jumpei Teramachi, Tatsuji Haneji Posttranslational modifications including histone methylation regulate gene transcription through directly affecting the structure of chromatin. Trimethylation of histone H3K27 (H3K27me3) contributes to gene silencing and the histone demethylase Jumonji domain-containing 3 (Jmjd3) specifically removes the methylation of H3K27me3, followed by the activation of gene expression. In the present study, we explored the roles of Jmjd3 i...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 13, 2016 Category: Molecular Biology Source Type: research

Direct non transcriptional role of NF-Y in DNA replication
Publication date: Available online 28 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Paolo Benatti, Silvia Belluti, Benoit Miotto, Julia Neusiedler, Diletta Dolfini, Marjorie Drac, Valentina Basile, Etienne Schwob, Roberto Mantovani, J. Julian Blow, Carol Imbriano NF-Y is a heterotrimeric transcription factor, which plays a pioneer role in the transcriptional control of promoters containing the CCAAT-box, among which genes involved in cell cycle regulation, apoptosis and DNA damage response. The knock-down of the sequence-specific subunit NF-YA triggers defects in...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

Clock genes-dependent acetylation of complex I sets rhythmic activity of mitochondrial OxPhos
Publication date: Available online 28 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Olga Cela, Rosella Scrima, Valerio Pazienza, Giuseppe Merla, Giorgia Benegiamo, Bartolomeo Augello, Sabino Fugetto, Marta Menga, Rosa Rubino, Luise Fuhr, Angela Relógio, Claudia Piccoli, Gianluigi Mazzoccoli, Nazzareno Capitanio Physiology of living beings show circadian rhythms entrained by a central timekeeper present in the hypothalamic suprachiasmatic nuclei. Nevertheless, virtually all peripheral tissues hold autonomous molecular oscillators constituted essentially ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

CXCR4 signaling is controlled by immobilization at the plasma membrane
Publication date: Available online 31 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Elena Beletkaia, Susanne F. Fenz, Wim Pomp, Ewa Snaar-Jagalska, Pancras W.C. Hogendoorn, Thomas Schmidt Understanding of the regulation mechanisms of CXCR4 signaling is essential for revealing its role in physiological and pathological processes. Though biochemical pathways following CXCR4 activation by its ligand CXCL12 are well established, knowledge about the receptor dynamics on the plasma membrane remains limited. Here we used Ewing sarcoma-derived cells to unravel the processes t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

Characterization, prediction and evolution of plant peroxisomal targeting signals type 1 (PTS1s)
Publication date: Available online 6 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): S. Reumann, G. Chowdhary, T. Lingner Our knowledge of the proteome of plant peroxisomes and their functional plasticity is far from being complete, primarily due to major technical challenges in experimental proteome research of the fragile cell organelle. Several unexpected novel plant peroxisome functions, for instance in biotin and phylloquinone biosynthesis, have been uncovered recently. Nevertheless, very few regulatory and membrane proteins of plant peroxisomes have been identified an...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

ER functions of oncogenes and tumor suppressors: Modulators of intracellular Ca2+ signaling
This article is part of a Special Issue entitled: Calcium and Cell Fate. Guest Editors: Dr. Jacques Haiech, Professor Heizmann, Professor Joachim Krebs. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

Cellular functions of programmed cell death 5
Publication date: Available online 8 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Ge Li, Dalong Ma, Yingyu Chen Programmed Cell Death 5 (PDCD5) was originally identified as an apoptosis-accelerating protein that is widely expressed and has been well conserved during the process of evolution. PDCD5 has complex biological functions, including programmed cell death and immune regulation. It can accelerate apoptosis in different type of cells in response to different stimuli. During this process, PDCD5 rapidly translocates from the cytoplasm to the nucleus. PDCD5 regulates t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

Small GTPases in peroxisome dynamics
This article is part of a Special Issue entitled: Peroxisomes. Guest Editors: Ralf Erdmann. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

mTOR, cardiomyocytes and inflammation in cardiac hypertrophy
This article is part of a Special Issue entitled:Cardiomyocyte Biology: Integration of Develomental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

Regulation of peroxisome dynamics by phosphorylation
This article is part of a Special Issue entitled: Peroxisomes edited by Ralf Erdmann. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

miR-29c-3p promotes senescence of human mesenchymal stem cells by targeting CNOT6 through p53-p21 and p16-pRB pathways
In conclusion, our results demonstrate that miR-29c-3p promotes the senescence of MSCs by targeting CNOT6 through p53-p21 and p16-pRB pathways and highlight the contribution of post-transcriptional regulation to stem cell senescence. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research

CRAC channels, calcium, and cancer in light of the driver and passenger concept
Publication date: Available online 17 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Markus Hoth (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 25, 2015 Category: Molecular Biology Source Type: research

A long non-coding RNA, BC048612 and a microRNA, miR-203 coordinate the gene expression of Neuronal growth regulator 1 (NEGR1) adhesion protein
Publication date: Available online 23 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Prameet Kaur, Tan Jun Rong, Dwi Setyowati Karolina, Sugunavathi Sepramaniam, Arunmozhiarasi Armugam, Peter Wong Tsun-Hon, Kandiah Jeyaseelan The regulatory roles for non-coding RNAs, the long non-coding RNAs and microRNAs, are emerging as crucial determinants of central nervous system development and function. Neuronal growth regulator 1 (NEGR1) is a cell adhesion molecule that has been shown to play an important role in neurite outgrowth during neuronal development. Precise expressio...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 25, 2015 Category: Molecular Biology Source Type: research

Glutamine Transporters in Mammalian Cells and Their Functions in Physiology and Cancer
This article is part of a Special Issue entitled: Mitochondrial Channels edited by Jean-Claude Martinou. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 25, 2015 Category: Molecular Biology Source Type: research

Corrigendum to “Dominant negative Ras attenuates pathological ventricular remodeling in pressure overload cardiac hypertrophy” [Biochim. Biophys. Acta 1853/11 (2015) 2870–2884]
Publication date: Available online 22 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Manuel Ramos-Kuri, Kleopatra Rapti, Hind Mehel, Shihong Zhang, Perundurai S. Dhandapany, Lifan Liang, Alejandro García-Carrancá, Regis Bobe, Rodolphe Fischmeister, Serge Adnot, Djamel Lebeche, Roger J. Hajjar, Larissa Lipskaia, Elie R. Chemaly (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 23, 2015 Category: Molecular Biology Source Type: research

Introduction to tumor microenvironment regulation of cancer cell survival, metastasis, inflammation, and immune surveillance
Publication date: Available online 23 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Gregg L. Semenza, Peter P. Ruvolo (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 23, 2015 Category: Molecular Biology Source Type: research

AT-101 simultaneously triggers apoptosis and a cytoprotective type of autophagy irrespective of expression levels and the subcellular localisation of Bcl-xL and Bcl-2 in MCF7 cells
Publication date: Available online 22 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): P. Antonietti, F. Gessler, H. Düssmann, C. Reimertz, M. Mittelbronn, J.H.M. Prehn, D. Kögel The effects of autophagy on cell death are highly contextual and either beneficial or deleterious. One prime example for this dual function of autophagy is evidenced by the cell responses to the BH3 mimetic AT-101 that is known to induce either apoptotic or autophagy-dependent cell death in different settings. Based on previous reports, we hypothesized that the expression levels of pr...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 22, 2015 Category: Molecular Biology Source Type: research

Cardiac voltage-gated Calcium Channel, macromolecular complexes
This article is part of a Special Issue entitled:Cardiomyocyte Biology: Integration of Develomental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel." (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 19, 2015 Category: Molecular Biology Source Type: research

The Signaling module cAMP/epac/Rap1/PLCε/IP3 mobilizes acrosomal calcium during sperm exocytosis
Publication date: Available online 17 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Ornella Lucchesi, María C. Ruete, Matías A. Bustos, María F. Quevedo, Claudia N. Tomes Exocytosis of the sperm's single secretory granule, or acrosome, is a regulated exocytosis triggered by components of the egg's investments. In addition to external calcium, sperm exocytosis (termed the acrosome reaction) requires cAMP synthesized endogenously and calcium mobilized from the acrosome through IP3-sensitive channels. The relevant cAMP target is Epac. In the first par...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2015 Category: Molecular Biology Source Type: research

Multifaceted plasma membrane Ca2+ pumps: From structure to intracellular Ca2+ handling and cancer
This article is part of a Special Issue entitled: Calcium and Cell Fate edited by Jacques Haiech, Claus Heizmann and Joachim Krebs. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2015 Category: Molecular Biology Source Type: research

CRAC channels, calcium and cancer in light of the driver and passenger concept
Publication date: Available online 17 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Markus Hoth (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2015 Category: Molecular Biology Source Type: research

Combination of galectin inhibitor GCS-100 and BH3 mimetics eliminates both p53 wild type and p53 null AML cells
In this study, we found GCS-100 induced apoptosis in AML cells. The agent reduced MCL-1 expression suggesting GCS-100 could be more effective when combined with a BH3 mimetic. Indeed, potent synergistic cytotoxicity was achieved when GCS-100 was combined with ABT-737 or ABT-199. Furthermore, the GCS-100/ABT-199 combination was effective against primary AML blast cells from patients with FLT3 ITD mutations, which is another prognostic factor for poor outcome in AML. This activity may involve wild-type p53 as shRNA knockdown of LGALS3 or galectin 1 (LGALS1) sensitized wild-type p53 OCI-AML3 cells to GCS-100/ABT-737-induced a...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2015 Category: Molecular Biology Source Type: research

Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress
In conclusion, MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2015 Category: Molecular Biology Source Type: research

Methods for in vitro functional analysis of iPSC derived cardiomyocytes - Special focus on analyzing the mechanical beating behavior
This article is part of a Special Issue entitled:Cardiomyocyte Biology: Integration of Develomental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2015 Category: Molecular Biology Source Type: research

Gemcitabine and mitomycin induced autophagy regulates cancer stem cell pool in urothelial carcinoma cells
Publication date: February 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1863, Issue 2 Author(s): Rani Ojha, V. Jha, S.K. Singh Urothelial carcinoma (UC) is characterized by therapeutic resistance and frequent tumor relapse. It has been suggested that UC are driven by a rare subset of cancer stem cells (CSCs). In order to understand UC recurrence post therapy, we investigated the behavior of urothelial CSCs after exposure to commonly used chemotherapeutic agents, gemcitabine (GC) and mitomycin (MM). Although, the role of autophagy in CSC maintenance is well documented, the relations...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 17, 2015 Category: Molecular Biology Source Type: research

Peroxisomes in brain development and function
This article is part of a Special Issue entitled: Peroxisomes edited by Ralf Erdmann. 1 1 This article is part of a Special Issue entitled: Peroxisomes edited by Ralf Erdmann. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 11, 2015 Category: Molecular Biology Source Type: research

Multiplex detection of protein–protein interactions using a next generation luciferase reporter
Publication date: February 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1863, Issue 2 Author(s): Lisette G.G.C. Verhoef, Michela Mattioli, Fernanda Ricci, Yao-Cheng Li, Mark Wade Cell-based assays of protein–protein interactions (PPIs) using split reporter proteins can be used to identify PPI agonists and antagonists. Generally, such assays measure one PPI at a time, and thus counterscreens for on-target activity must be run in parallel or at a subsequent stage; this increases both the cost and time during screening. Split luciferase systems offer advantages over those that...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 11, 2015 Category: Molecular Biology Source Type: research

Normal hematopoiesis and lack of β-catenin activation in osteoblasts of patients and mice harboring Lrp5 gain of function mutations
Publication date: Available online 8 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Marta Galán-Díez, Adiba Isa, Marco Ponzetti, Morten Frost Nielsen, Moustapha Kassem, Stavroula Kousteni Osteoblasts are emerging regulators of myeloid malignancies since genetic alterations in them, such as constitutive activation of β-catenin, instigate their appearance. The LDL receptor-related protein 5 (LRP5), initially proposed to be a co-receptor for Wnt proteins, in fact favors bone formation by suppressing gut-serotonin synthesis. This function of Lrp5 occurri...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 9, 2015 Category: Molecular Biology Source Type: research

Generation of cardiac pacemaker cells by programming and differentiation
Publication date: Available online 8 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Britta Husse, Wolfgang-Michael Franz A number of diseases is caused by faulty function of the cardiac pacemaker and described as “sick sinus syndrome”. The medical treatment of sick sinus syndrome with electrical pacemaker implants in the diseased heart includes risks. These problems may be overcome via “biological pacemaker” derived from different adult cardiac cells or pluripotent stem cells. The generation of cardiac pacemaker cells requires the understanding of t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 9, 2015 Category: Molecular Biology Source Type: research

The E3 ubiquitin ligase CHIP mediates ubiquitination and proteasomal degradation of PRMT5
Publication date: Available online 2 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Huan-Tian Zhang, Ling-Fei Zeng, Qing-Yu He, W. Andy Tao, Zhen-Gang Zha, Chang-Deng Hu Protein arginine methyltransferase 5 (PRMT5) is an important member of the protein arginine methyltransferase family that regulates many cellular processes through epigenetic control of target gene expression. Because of its overexpression in a number of human cancers and its essential role in cell proliferation, transformation, and cell cycle progression, PRMT5 has been recently proposed to function a...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2015 Category: Molecular Biology Source Type: research

Gemcitabine and mitomycin induced autophagy regulates cancer stem cells pool in urothelial carcinoma cells
Publication date: Available online 2 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Rani Ojha, V. Jha, S.K. Singh Urothelial carcinoma (UC) is characterized by therapeutic resistance and frequent tumor relapse. It has been suggested that UC are driven by a rare subset of cancer stem cells (CSCs). In order to understand UC recurrence post therapy, we investigated the behavior of urothelial CSCs after exposure to commonly used chemotherapeutic agents, gemcitabine (GC) and mitomycin (MM). Although, the role of autophagy in CSC maintenance is well documented; but the relation...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2015 Category: Molecular Biology Source Type: research

Depolarized FRET (depolFRET) on the cell surface: FRET control by photoselection
Publication date: Available online 3 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): László Bene, Péter Gogolák, Tamás Ungvári, Miklós Bagdány, István Nagy, László Damjanovich Sensitivity of FRET in hetero- and homo-FRET systems on the photoselected orientation distribution of donors have been proven by using polarized and depolarized light for excitation. FRET as well as donor and acceptor anisotropies have been simultaneously measured in a dual emission-polarization scheme realized in a conve...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2015 Category: Molecular Biology Source Type: research

Corrigendum to “IRS2 and PTEN are key molecules in controlling insulin sensitivity in podocytes” [Biochim. Biophys. Acta 1853 (12) (2015) 3224–3234]
Publication date: Available online 3 December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Beatriz Santamaria, Eva Marquez, Abigail Lay, RoseaMarie M. Carew, Águeda González-Rodríguez, Gavin I. Welsh, Lan Ni, Lorna J. Hale, Alberto Ortiz, Moin A. Saleem, Derek P. Brazil, Richard J. Coward, Ángela M. Valverde (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2015 Category: Molecular Biology Source Type: research