A novel mutation in the promoter region of RPL8 regulates milk fat traits in dairy cattle by binding transcription factor Pax6
Publication date: Available online 11 September 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Xian-Rui Zheng, Li Jiang, Chao Ning, Zheng-Zheng Hu, Lei Zhou, Ying Yu, Sheng-Li Zhang, Jian-Feng LiuAbstractRibosomal protein L8 (RPL8) was considered as a promising candidate gene for the milk fat percentage trait in dairy cattle in our previous genome-wide association studies, but the mechanism remains to be determined. Here we investigated the molecular mechanism underlying the effect of bovine RPL8 on milk fat percentage. We demonstrated that RPL8 silencing in bovine mammary ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 12, 2019 Category: Lipidology Source Type: research

Autophagy differentially regulates macrophage lipid handling depending on the lipid substrate (oleic acid vs. acetylated-LDL) and inflammatory activation state
Publication date: Available online 12 September 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Sapir Hadadi-Bechor, Yulia Haim, Tal Pecht, Roni Gat, Tanya Tarnovsky, Martin Gericke, Assaf RudichAbstractThe regulation of lipid droplet (LD) dynamics by autophagy in naïve macrophages is complex: Inhibiting autophagosome initiation steps attenuates oleic acid (OA) induced LD (OA-LD) biogenesis, whereas interfering with later-autophagosome maturation/lysosomal steps accelerates OA-LD biogenesis rate, but not OA-LD degradation. Here we hypothesized that regulation of macroph...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 12, 2019 Category: Lipidology Source Type: research

Transplantation of human adipose tissue derived-SVF enhance liver function through high anti-inflammatory property
Publication date: Available online 12 September 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Ja Sung Choi, Dong-Sik Chae, Hyun Aae Ryu, Sung-Whan KimAbstractAlthough human adipose tissue-derived stromal vascular fraction (SVF) has been considered a promising source of stem cells, its characteristics relevant to treatment of a damaged liver have not been fully elucidated. In the present study, we sought to characterize the property of human SVF and determine the therapeutic utility of SVF in the liver cirrhosis model. We performed microarray, quantitative (q)-PCR experimen...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 12, 2019 Category: Lipidology Source Type: research

Decreased H3K9 acetylation level of LXRα mediated dexamethasone-induced placental cholesterol transport dysfunction
This study aimed to investigate the effects of dexamethasone on fetal cholesterol levels, and explore its placental mechanism. Pregnant Wistar rats were injected subcutaneously with dexamethasone (0.8 mg/kg·d) from gestational day 9 to 20. Results showed that dexamethasone increased maternal serum total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C) levels, as well as placental cholesterol synthesis and TC concentration, while reduced fetal birth weight, and serum TC, HDL-C and LDL-C levels. Meanwhile, the expression of placental cholesterol transporters...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 10, 2019 Category: Lipidology Source Type: research

Uptake of HDL-cholesterol contributes to lipid accumulation in clear cell renal cell carcinoma
In this study, we analyzed 42 primary ccRCC samples, and determined that cholesterol levels of ~ 70% of the tumors were at least two-fold higher than that of benign kidney tissues. Compared to tumors without cholesterol accumulation, those containing excess cholesterol expressed higher levels of scavenger receptor BI (SR-B1), a receptor for uptake of HDL-associated cholesterol, but not genes involved in cholesterol synthesis and uptake of LDL-associated cholesterol. To further determine the roles of sterol accumulation for cancer development, we implanted ccRCC from patients into mouse kidneys using a mouse ccRCC xenograft...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 9, 2019 Category: Lipidology Source Type: research

Lipid accumulation impairs lysosomal acid lipase activity in hepatocytes: Evidence in NAFLD patients and cell cultures
ConclusionsLAL activity is reduced in NAFLD patients, independently from disease progression. In vitro, impaired LAL activity induced by FA loading was rescued by PPAR-alpha activation. These data suggest that the pharmacological modulation of LAL should be explored in the management of NAFLD patients. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 9, 2019 Category: Lipidology Source Type: research

Regulation of the amount of ceramide-1-phosphate synthesized in differentiated human podocytes
Publication date: Available online 2 September 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Shamroop kumar Mallela, Alla Mitrofanova, Sandra Merscher, Alessia FornoniAbstractSphingolipids have important functions as structural components of cells but they also function as signaling molecules regulating different cellular processes such as apoptosis, cell proliferation, cell migration, cell division and inflammation. Hence, a tight regulation of the sphingolipid homeostasis is essential to maintain proper cellular functions. Mammalian ORMDL proteins are orthologues of the ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 4, 2019 Category: Lipidology Source Type: research

Adaptation of Synechococcus sp. PCC 7942 to phosphate starvation by glycolipid accumulation and membrane lipid remodeling
This study provides valuable insight into cyanobacteria adaptation strategies to phosphate stress by membrane lipid remodeling and unveils the underlying acyl chain fluxes into glycolipids. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 2, 2019 Category: Lipidology Source Type: research

Low profile high value target: The role of OxLDL in cancer
Publication date: Available online 31 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Albert V. Bitorina, Yvonne Oligschlaeger, Ronit Shiri-Sverdlov, Jan TheysAbstractUnhealthy Western-type diet and physical inactivity are highly associated with the current obesity epidemic and its related metabolic diseases such as atherosclerosis and non-alcoholic steatohepatitis. In addition, increasing evidence indicates that obesity is also a major risk factor for several types of common cancers. Recent studies that have provided correlative support that disturbed lipid metabolis...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - September 1, 2019 Category: Lipidology Source Type: research

Estrogen-related receptor γ controls sterol regulatory element-binding protein-1c expression and alcoholic fatty liver
In this study, we investigated the role of ERRγ in alcohol-mediated hepatic lipogenesis and examined the possibility to ameliorate alcoholic fatty liver through its inverse agonist. Hepatic ERRγ and SREBP-1c expression was increased by alcohol-mediated activation of CB1 receptor signaling. Deletion and mutation analyses of the Srebp-1c gene promoter showed that ERRγ directly regulates Srebp-1c gene transcription via binding to an ERR-response element. Overexpression of ERRγ significantly induced SREBP-1c expression and fat accumulation in liver of mice, which were blocked in Srebp-1c-knockout hepato...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 31, 2019 Category: Lipidology Source Type: research

Omega hydroxylated JA-Ile is an endogenous bioactive jasmonate that signals through the canonical jasmonate signaling pathway
This study shows that ω-hydroxylated JA-Ile (12-OH-JA-Ile) retains bioactivity and signals through the canonical JA-pathway. The results suggest that 12-OH-JA-Ile differentially activates a subset of JA-Ile co-receptors that may control and/or modulate particular jasmonate dependent responses. It is proposed that after a strong immune response mediated by JA-Ile, the ω-hydroxylated form modulates JA-Ile activated processes thereby improving plant resilience. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 31, 2019 Category: Lipidology Source Type: research

Exercise regulates lipid droplet dynamics in normal and fatty liver
Publication date: Available online 29 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Francisco Pino-de la Fuente, Laura Quezada, Carlos Sepúlveda, Matías Monsalves, Juan M. Rodríguez, Camila Sacristán, Mario Chiong, Miguel Llanos, Alejandra Espinosa, Rodrigo TroncosoAbstractLipids droplets (LD) are dynamics organelles that accumulate neutral lipids during nutrient surplus. LD alternates between periods of growth and consumption through regulated processes including as de novo lipogenesis, lipolysis and lipophagy. The liver is a central tis...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 31, 2019 Category: Lipidology Source Type: research

Metabolic labelling of choline phospholipids probes ABCA3 transport in lamellar bodies
Publication date: Available online 29 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Yang Li, Susanna Kinting, Stefanie Höppner, Maria Elisabeth Forstner, Olaf Uhl, Berthold Koletzko, Matthias GrieseAbstractIn the metabolism of pulmonary surfactant, the ATP-binding cassette sub-family A member 3 (ABCA3) is a crucial protein in the formation of the storage compartment for surfactant, the lamellar body (LB), and the transport of phospholipids in it. Mutations in ABCA3 not only disturb surfactant metabolism but also cause chronic interstitial lung diseases. Assays ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 31, 2019 Category: Lipidology Source Type: research

Intracellular Ca2+-dependent formation of N-acyl-phosphatidylethanolamines by human cytosolic phospholipase A2ε
Publication date: Available online 29 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Smriti Sultana Binte Mustafiz, Toru Uyama, Katsuya Morito, Naoko Takahashi, Katsuhisa Kawai, Zahir Hussain, Kazuhito Tsuboi, Nobukazu Araki, Kei Yamamoto, Tamotsu Tanaka, Natsuo UedaAbstractN-Acyl-phosphatidylethanolamines (NAPEs) are known to be precursors of bioactive N-acylethanolamines (NAEs), including the endocannabinoid arachidonoylethanolamide (anandamide) and anti-inflammatory palmitoylethanolamide. In mammals, NAPEs are produced by N-acyltransferases, which transfer an acyl...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 31, 2019 Category: Lipidology Source Type: research

Conditionally immortalized brown preadipocytes can switch between proliferative and differentiated states
In this study, monoclonal lines of conditionally immortalized brown preadipocytes (iBPAs) of mouse and human origin were generated. Conditional immortalization was achieved by doxycycline-controlled expression of simian virus 40 large tumor antigen (LT) with a repressor-based Tet-On system. In the presence of doxycycline, both the murine and human cell lines showed long-term proliferation capacity with a population doubling time of ~28 h. After switching off LT expression by doxycycline removal and exposure to adipogenic differentiation medium, cells from both species acquired brown adipocyte properties. This was evidenced...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 28, 2019 Category: Lipidology Source Type: research

Common aspects in the engineering of yeasts for fatty acid- and isoprene-based products
Publication date: Available online 26 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Simon Arhar, Klaus NatterAbstractThe biosynthetic pathways for most lipophilic metabolites share several common principles. These substances are built almost exclusively from acetyl-CoA as the donor for the carbon scaffold and NADPH is required for the reductive steps during biosynthesis. Due to their hydrophobicity, the end products are sequestered into the same cellular compartment, the lipid droplet. In this review, we will summarize the efforts in the metabolic engineering of yea...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 28, 2019 Category: Lipidology Source Type: research

KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α
Publication date: Available online 26 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Luciano Pirone, Giovanni Smaldone, Rosa Spinelli, Manlio Barberisi, Francesco Beguinot, Luigi Vitagliano, Claudia Miele, Sonia Di Gaetano, Gregory Alexander Raciti, Emilia PedoneAbstractAdipogenesis has an important role in regulating energy balance, tissue homeostasis and disease pathogenesis. 3T3-L1 preadipocytes have been widely used as an in vitro model for studying adipocyte differentiation. We here show that KCTD1, a member of the potassium channel containing tetramerization do...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 27, 2019 Category: Lipidology Source Type: research

Anandamide targets aromatase: A breakthrough on human decidualization
In this study, we hypothesized whether AEA inhibitory effect on cell decidualization could be mediated through interaction with aromatase and consequent interference in estradiol production/signaling. We used an immortalized human endometrial stromal cell line (St-T1b) and human decidual fibroblasts (HdF) derived from human term placenta. In cells exposed to a differentiation stimulus, AEA-treatment prevents the increase of the expression of CYP19A1 gene encoding aromatase, E2 levels and of estradiol receptor expression, that are observed in differentiating cells. Regarding CYP19A1 mRNA levels, the effect was partially rev...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 25, 2019 Category: Lipidology Source Type: research

FAM134B improves preadipocytes differentiation by enhancing mitophagy
In this study, we provide mechanistic links between FAM134B and adipocyte differentiation. Here, we found that adipocyte-specific FAM134B overexpression mice are obese and have increased white adipose tissue (WAT) mass. Serum tests showed that they developed high glucose level and severe insulin resistance. In addition, they also exhibited enhanced autophagy and reduced mitochondria amount, suggesting the function of FAM134B to promote autophagy in adipocytes. Overexpression of FAM134B in 3 T3-L1 preadipocytes promoted autophagy and differentiation, while the effect could be inhibited after treatment with autophagy ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 22, 2019 Category: Lipidology Source Type: research

Consulting prostate cancer cohort data uncovers transcriptional control: Regulation of the MARCH6 gene
Publication date: Available online 15 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Hudson W. Coates, Ngee Kiat Chua, Andrew J. BrownAbstractCholesterol accumulation is a hallmark of prostate cancer (PCa) enabled by upregulation of its synthesis, which presents a potential therapeutic target. This pathway is suppressed by the E3 ubiquitin ligase membrane-associated RING-CH-type finger 6 (MARCH6); however, little is known of MARCH6 regulation, particularly at the transcriptional level. Here, we consulted large transcriptomic PCa datasets to investigate transcription ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 17, 2019 Category: Lipidology Source Type: research

Phosphorylation of the lipid droplet localized glycerol‑3‑phosphate acyltransferase Gpt2 prevents a futile triacylglycerol cycle in yeast
Publication date: Available online 14 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Bernadette Kiegerl, Marjan Tavassoli, Heather Smart, Brittney N. Shabits, Vanina Zaremberg, Karin AthenstaedtAbstractThe proteome of lipid droplets, storage compartments of triacylglycerols (TAGs), comprises TAG synthesizing and TAG degrading enzymes. Thus, to prevent a futile cycle the activity of enzymes catalyzing key steps in TAG turnover has to be strictly coordinated. The first and committed reaction of TAG synthesis is catalyzed by a glycerol‑3‑phosphate acyltransferase (G...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 15, 2019 Category: Lipidology Source Type: research

Gene identification and functional characterization of a Δ12 fatty acid desaturase in Tetrahymena thermophila and its influence in homeoviscous adaptation to low temperature
Publication date: Available online 14 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Maria L. Sanchez Granel, Camila Cánepa, Nicolas G. Cid, Juan C. Navarro, Óscar Monroig, Sandra V. Verstraeten, Clara B. Nudel, Alejandro D. NusblatAbstractHomeoviscous adaptation in poikilotherms is based in the regulation of the level of desaturation of fatty acids, variation in phospholipids head groups and sterol content in the membrane lipids, in order to maintain the membrane fluidity in response to changes in environmental temperature. Increased proportion of unsa...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 15, 2019 Category: Lipidology Source Type: research

Corrigendum to “PIP2 epigenetically represses rRNA genes transcription interacting with PHF8” [Biochim. Biophys. Acta Mol. Cell Biol. Volume 1863/3 2017 266-275]
Publication date: Available online 12 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Livia Ulicna, Alzbeta Kalendova, Ilona Kalasova, Tomas Vacik, Pavel Hozák (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 12, 2019 Category: Lipidology Source Type: research

The ligand-mediated affinity of brain-type fatty acid-binding protein for membranes determines the directionality of lipophilic cargo transport
Publication date: Available online 9 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Yi-Yun Cheng, Yun-Fang Huang, Hsin-Hui Lin, Wun-Shaing Wayne Chang, Ping-Chiang LyuAbstractThe intracellular transport of lipophilic cargoes is a highly dynamic process. In eukaryotic cells, the uptake and release of long-chain fatty acids (LCFAs) are executed by fatty-acid binding proteins. However, how these carriers control the directionality of cargo trafficking remains unclear. Here, we revealed that the unliganded archetypal Drosophila brain-type fatty acid-binding protein (dFAB...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 10, 2019 Category: Lipidology Source Type: research

The fate of medium-chain fatty acids in very long-chain acyl‑CoA dehydrogenase deficiency (VLCADD): A matter of sex?
Publication date: Available online 5 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Zeinab Wehbe, Khaled Alatibi, Julia Jellusova, Ute Spiekerkoetter, Sara TucciAbstractMedium-chain-triglycerides (MCT) are widely applied in the treatment of long-chain fatty acid oxidation disorders (lcFAOD). Long-term treatment with MCT led to a sexually dimorphic response in the mouse model of very-long-chain-acyl-CoA-dehydrogenase-deficiency (VLCAD−/−) with the subsequent development of a metabolic syndrome in female mice.In order to evaluate the molecular mechanisms re...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 6, 2019 Category: Lipidology Source Type: research

The emerging role of the mitochondrial fatty-acid synthase (mtFASII) in the regulation of energy metabolism
Publication date: Available online 31 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Zeinab Wehbe, Sidney Behringer, Khaled Alatibi, David Watkins, David Rosenblatt, Ute Spiekerkoetter, Sara TucciAbstractMalonyl-CoA synthetase (ACSF3) catalyzes the first step of the mitochondrial fatty acid biosynthesis (mtFASII). Mutations in ACSF3 cause CMAMMA a rare inborn error of metabolism. The clinical phenotype is very heterogeneous, with some patients presenting with neurologic manifestations. In some children, presenting symptoms such as coma, ketoacidosis and hypoglycemia ar...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 2, 2019 Category: Lipidology Source Type: research

Long-chain acyl-CoA synthetase 4 participates in the formation of highly unsaturated fatty acid-containing phospholipids in murine macrophages
Publication date: Available online 31 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Hiroshi Kuwata, Eriko Nakatani, Satoko Shimbara-Matsubayashi, Fumihiro Ishikawa, Motoko Shibanuma, Yuka Sasaki, Emiko Yoda, Yoshihito Nakatani, Shuntaro HaraAbstractLong-chain acyl-coenzyme A synthetases (ACSLs) are a family of enzymes that convert free long-chain fatty acids into their acyl-coenzyme A (CoA) forms. ACSL4, belonging to the ACSL family, shows a preferential use of arachidonic acid (AA) as its substrate and plays a role in the remodeling of AA-containing phospholipids by ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - August 2, 2019 Category: Lipidology Source Type: research

Editorial Board
Publication date: October 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1864, Issue 10Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 31, 2019 Category: Lipidology Source Type: research

Spatial compartmentalization of lipid droplet biogenesis
Publication date: Available online 25 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Mike Henne, Joel M. Goodman, Hanaa HaririAbstractLipid droplets (LDs) are ubiquitous organelles that store metabolic energy in the form of neutral lipids (typically triacylglycerols and steryl esters). Beyond being inert energy storage compartments, LDs are dynamic organelles that participate in numerous essential metabolic functions. Cells generate LDs de novo from distinct sub-regions at the endoplasmic reticulum (ER), but what determines sites of LD formation remains a key unanswere...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 26, 2019 Category: Lipidology Source Type: research

A dual role of 12/15-lipoxygenase in LPS-induced acute renal inflammation and injury
Publication date: Available online 23 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Ahmed A. Elmarakby, Ahmed S. Ibrahim, Mohamed A. Katary, Nehal M. Elsherbini, Mohamed El-Shafey, Ahmed M. Abd-Elrazik, Rafik A. Abdelsayed, Krishna Rao Maddipati, Mohamed Al-ShabraweyAbstractRecent studies suggest a potential role of bioactive lipids in acute kidney injury induced by lipopolysaccharide (LPS). The current study was designed to determine the profiling activities of various polyunsaturated fatty acid (PUFA) metabolizing enzymes, including lipoxygenases (LO), cyclooxygenas...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 24, 2019 Category: Lipidology Source Type: research

Specialized ER membrane domains for lipid metabolism and transport
Publication date: Available online 23 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Taki Nishimura, Christopher J. StefanAbstractThe endoplasmic reticulum (ER) is a highly organized organelle that performs vital functions including de novo membrane lipid synthesis and transport. Accordingly, numerous lipid biosynthesis enzymes are localized in the ER membrane. However, it is now evident that lipid metabolism is sub-compartmentalized within the ER and that lipid biosynthetic enzymes engage with lipid transfer proteins (LTPs) to rapidly shuttle newly synthesized lipids ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 24, 2019 Category: Lipidology Source Type: research

Gut microbial metabolites of linoleic acid are metabolized by accelerated peroxisomal β-oxidation in mammalian cells
In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 h. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2– and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HY...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 24, 2019 Category: Lipidology Source Type: research

The lncRNA ENST00000608794 acts as a competing endogenous RNA to regulate PDK4 expression by sponging miR-15b-5p in dexamethasone induced steatosis
Publication date: Available online 19 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Fengqiong Liu, Qing Chen, Fa Chen, Jing Wang, Ruijie Gong, Baochang HeAbstractThe contribution of ncRNAs, especially long non-coding RNAs (lncRNAs) to drug induced steatosis remains largely unknown. The aim of this study was to investigate the role of lncRNA ENST00000608794 in dexamethasone induced steatosis. We found that ENST00000608794 is expressed at higher levels in dexamethasone treated HepG2 cell, and ENST00000608794 can bind and be regulated by miR-15b-5p. Ectopic expression of...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 20, 2019 Category: Lipidology Source Type: research

Detection of unprecedented allene oxide synthase member of CYP74B subfamily: CYP74B33 of carrot (Daucus carota)
Publication date: Available online 19 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Svetlana S. Gorina, Fakhima K. Mukhitova, Tatiana M. Ilyina, Yana Y. Toporkova, Alexander N. GrechkinAbstractEnzymes of CYP74 family widespread in higher plants control the metabolism of fatty acid hydroperoxides to numerous bioactive oxylipins. Hydroperoxide lyases (HPLs, synonym: hemiacetal synthases) of CYP74B subfamily belong to the most common CYP74 enzymes. HPLs isomerize the hydroperoxides to the short-lived hemiacetals, which are spontaneously decomposed to aldehydes and aldoac...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 20, 2019 Category: Lipidology Source Type: research

Hepatocyte peroxisome proliferator-activated receptor α regulates bile acid synthesis and transport
Publication date: October 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1864, Issue 10Author(s): Cen Xie, Shogo Takahashi, Chad N. Brocker, Shijun He, Li Chen, Guomin Xie, Katrina Jang, Xiaoxia Gao, Kristopher W. Krausz, Aijuan Qu, Moshe Levi, Frank J. GonzalezAbstractPeroxisome proliferator-activated receptor alpha (PPARα) controls lipid homeostasis through regulation of lipid transport and catabolism. PPARα activators are clinically used for hyperlipidemia treatment. The role of PPARα in bile acid (BA) homeostasis is beginning to emerge. Herein, Ppara-nul...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 20, 2019 Category: Lipidology Source Type: research

Ursodeoxycholic acid is a GPBAR1 agonist and resets liver/intestinal FXR signaling in a model of diet-induced dysbiosis and NASH
ConclusionsUDCA is a GPBAR1 ligand and exerts beneficial effects in a rodent model of NASH by activating non-overlapping pathway with OCA. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 18, 2019 Category: Lipidology Source Type: research

A novel enantioselective SGNH family esterase (NmSGNH1) from Neisseria meningitides: Characterization, mutational analysis, and ester synthesis
Publication date: Available online 17 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): W.i Yoo, Ly Thi Huong Luu Le, Jun Hyuck Lee, Kyeong Kyu Kim, T. Doohun KimAbstractIn Neisseria sp., SGNH family esterases are involved in bacterial pathogenesis as well as cell wall peptidoglycan maturation. Here, a novel enantioselective SGNH family esterase (NmSGNH1) from Neisseria meningitidis, which has sequence similarity to carbohydrate esterase (CE3) family, was catalytically characterized and functionally explored. NmSGNH1 exhibited a wide range of substrate specificities inclu...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 18, 2019 Category: Lipidology Source Type: research

Yeast phosphatidylinositol transfer protein Pdr17 does not require high affinity phosphatidylinositol binding for its cellular function
Publication date: Available online 11 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Zuzana Pevalová, Vladimír Pevala, Nicholas J. Blunsom, Dana Tahotná, Veronika Kotrasová, Roman Holič, Lucia Pokorná, Jacob A. Bauer, Eva Kutejová, Shamshad Cockcroft, Peter GriačAbstractYeast phosphatidylinositol transfer protein (PITP) Pdr17 is an essential component of the complex required for decarboxylation of phosphatidylserine (PS) to phosphatidylethanolamine (PE) at a non-mitochondrial location. According to current understanding, this...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 12, 2019 Category: Lipidology Source Type: research

Dietary fatty acid profile influences circulating and tissue fatty acid ethanolamide concentrations in a tissue-specific manner in male Syrian hamsters
ConclusionDFA composition influences FA and NAE levels across all tissues, leading to significant shifts in intestinal-brain OEA concentrations. The endogenously synthesized increased OEA levels in these tissues enable the gut-brain-interrelationship. Henceforth, we summarize that the brain transmits anorexic properties mediated via neuronal signalling, which may contribute to the maintenance of healthy body weight. Thus, the benefits of OEA can be enhanced by the inclusion of C18:1n9-enriched diets, pointing to the possible nutritional use of this naturally occurring bioactive lipid-amide in the management of obesity. (So...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 11, 2019 Category: Lipidology Source Type: research

Adipose lipidomics and RNA-Seq analysis revealed the enhanced mitochondrial function in UCP1 knock-in pigs
Publication date: Available online 2 July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Jianfei Pan, Cong Tao, Chunwei Cao, Qiantao Zheng, Sin Man Lam, Guanghou Shui, Xuexue Liu, Kui Li, Jianguo Zhao, Yanfang WangAbstractUncoupling protein 1 (UCP1) plays a key role in nonshivering thermogenesis and is involved in the pathogenesis of obesity. In a previous study, we generated adipocyte-specific UCP1 knock-in (UCP1-KI) pigs, which exhibited improved thermoregulatory ability and decreased fat deposition. To investigate whether UCP1 knock-in alters the lipid composition of adi...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 2, 2019 Category: Lipidology Source Type: research

Faraway, so close! Functions of Endoplasmic reticulum–Endosome contacts
This article is part of a Special Issue entitled Endoplasmic reticulum platforms for lipid dynamics edited by Shamshad Cockcroft and Christopher Stefan. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 26, 2019 Category: Lipidology Source Type: research

Lipids and their (un)known effects on ER-associated protein degradation (ERAD)
This article is part of a Special Issue entitled Endoplasmic reticulum platforms for lipid dynamics edited by Shamshad Cockcroft and Christopher Stefan. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 24, 2019 Category: Lipidology Source Type: research

The role of ceramide in regulating endoplasmic reticulum function
This article is part of a special issue entitled: ER Platforms for Membrane Lipid Dynamics. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 23, 2019 Category: Lipidology Source Type: research

Photosystem I oligomerization affects lipid composition in Synechocystis sp. PCC 6803
Conclusions drawn from this system should be generally applicable for protein-lipid interactions in biological membranes.Graphical abstractSchematic figure of thylakoid membrane structure in Synechocystis sp. PCC6803 wild type (WT) and PsaL mutant (PsaL protein-deficient, containing only PSI monomers). (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 20, 2019 Category: Lipidology Source Type: research

Phospholipid transfer protein (PLTP) deficiency attenuates high fat diet induced obesity and insulin resistance
Publication date: Available online 17 June 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Guohua Song, Chuanlong Zong, Mingzhu Shao, Yang Yu, Qian Liu, Hui Wang, Tingting Qiu, Peng Jiao, Zheng Guo, Phoebe Lee, Yi Luo, Xian-Cheng Jiang, Shucun QinAbstractIncreased phospholipid transfer protein (PLTP) activity has been found to be associated with obesity, and metabolic syndrome in humans. However, whether or not PLTP has a direct effect on insulin sensitivity and obesity is largely unknown. Here we analyzed the effect by using PLTP knockout (PLTP−/−) mouse model. ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 18, 2019 Category: Lipidology Source Type: research

Corrigendum to “Small-molecule inhibitors as potential therapeutics and as tools to understand the role of phospholipases A2” [Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864/6 2019 941-956]
Publication date: Available online 17 June 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Aikaterini Nikolaou, Maroula G. Kokotou, Sofia Vasilakaki, George Kokotos (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 18, 2019 Category: Lipidology Source Type: research

The role of lipins in innate immunity and inflammation
Publication date: Available online 17 June 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): María A. Balboa, Nagore de Pablo, Clara Meana, Jesús BalsindeAbstractLipins are phosphatidic acid phosphatase enzymes whose cellular function in regulating lipid metabolism has been known for decades, particularly in metabolically active tissues such as adipose tissue or liver. In recent years evidence is accumulating for key regulatory roles of the lipin family in innate immune cells. Lipins may help regulate signaling through relevant immune receptors such as Toll-like ...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 18, 2019 Category: Lipidology Source Type: research

Endothelial lipase increases antioxidative capacity of high-density lipoprotein
In conclusion, the augmented antioxidative capacity of EL-HDL is primarily determined by the enrichment of HDL with EL-generated lipolytic products and to a lesser extent by the decreased HDL particle size and the increased activity of chloramine T-sensitive mechanisms. (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 18, 2019 Category: Lipidology Source Type: research

Polyunsaturated fatty acids modify the extracellular vesicle membranes and increase the production of proresolving lipid mediators of human mesenchymal stromal cells
In this study, we supplemented hMSCs with polyunsaturated fatty acids (PUFAs); arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid, which serve as precursors for multiple LMs. We then determined the consequent compositional modifications in the fatty acid, phospholipid, and LM profiles. Mass spectrometric analyses revealed that the supplemented PUFAs were incorporated into the main membrane phospholipid classes with different dynamics, with phosphatidylcholine serving as the first acceptor. Most importantly, the PUFA modifications were transferred into hMSC-EVs, which are known to mediate hMSC immunomodulatio...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 15, 2019 Category: Lipidology Source Type: research

Editorial Board
Publication date: September 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1864, Issue 9Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids)
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 14, 2019 Category: Lipidology Source Type: research