The role of xanthophylls in the supramolecular organization of the photosynthetic complex LHCII in lipid membranes studied by high-resolution imaging and nanospectroscopy
Publication date: Available online 14 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Jiangtao Zhou, Sergey Sekatskii, Renata Welc, Giovani Dietler, Wieslaw I. GruszeckiAbstractThe xanthophyll cycle is a regulatory mechanism operating in the photosynthetic apparatus of plants. It consists of the conversion of the xanthophyll pigment violaxanthin to zeaxanthin, and vice versa, in response to light intensity. According to the current understanding, one of the modes of regulatory activity of the cycle is associated with the influence on a molecular organization of pigment-protein complexes. ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 16, 2019 Category: Biochemistry Source Type: research

The oxidation-reduction and electrocatalytic properties of CO dehydrogenase from Oligotropha carboxidovorans
Publication date: Available online 14 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Palraj Kalimuthu, Mélanie Petitgenet, Dimitri Niks, Stephanie Dingwall, Jeffrey R. Harmer, Russ Hille, Paul V. BernhardtAbstractCO dehydrogenase (CODH) from the Gram-negative bacterium Oligotropha carboxidovorans is a complex metalloenzyme of the xanthine oxidase family of molybdenum-containing enzymes, bearing a unique bimolecular Mo-S-Cu active site in addition to two [2Fe-2S] clusters (FeSI and FeSII) and one equivalent of FAD. CODH catalyzes the oxidation of CO to CO2 with the concomitant intr...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 15, 2019 Category: Biochemistry Source Type: research

Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry
Publication date: Available online 14 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Johan Berg, Jian Liu, Emelie Svahn, Shelagh Ferguson-Miller, Peter BrzezinskiAbstractData from earlier studies showed that minor structural changes at the surface of cytochrome c oxidase, near one of the proton-input pathways (the D pathway), result in dramatically decreased activity and a lower proton-pumping stoichiometry. To further investigate how changes around the D pathway orifice influence functionality of the enzyme, here we modified the nearby C-terminal loop of subunit I of the Rhodobacter sph...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 15, 2019 Category: Biochemistry Source Type: research

Instantaneous switching between different modes of non-photochemical quenching in plants. Consequences for increasing biomass production
Publication date: Available online 14 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Herbert van Amerongen, Jevgenij ChmeliovAbstractPhotosynthetic productivity usually saturates far below the maximum solar light intensity, meaning that in those conditions many absorbed photons and the resulting electronic excitations of the pigment molecules can no longer be utilized for photosynthesis. To avoid photodamage, various protection mechanisms are induced that dissipate excess excitations, which otherwise could lead to the formation of harmful molecular species like singlet oxygen. This Non-P...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 15, 2019 Category: Biochemistry Source Type: research

Comparative ultrafast spectroscopy and structural analysis of OCP1 and OCP2 from Tolypothrix
Publication date: Available online 14 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Valentyna Kuznetsova, Maria Agustina Dominguez-Martin, Han Bao, Sayan Gupta, Markus Sutter, Miroslav Kloz, Mateusz Rebarz, Martin Přeček, Yan Chen, Christopher J. Petzold, Corie Y. Ralston, Cheryl A. Kerfeld, Tomáš PolívkaAbstractThe orange carotenoid protein (OCP) is a structurally and functionally modular photoactive protein involved in cyanobacterial photoprotection. Recently, based on bioinformatic analysis and phylogenetic relationships, new families of OCP have been described...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 15, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 December 2019Source: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1860, Issue 12Author(s): (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 12, 2019 Category: Biochemistry Source Type: research

Integrated thermodynamic analysis of electron bifurcating [FeFe]-hydrogenase to inform anaerobic metabolism and H2 production
Publication date: Available online 8 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Zackary J. Jay, Kristopher A. Hunt, Katherine J. Chou, Gerrit J. Schut, Pin-Ching Maness, Michael W.W. Adams, Ross P. CarlsonAbstractElectron bifurcating, [FeFe]-hydrogenases are recently described members of the hydrogenase family and catalyze a combination of exergonic and endergonic electron exchanges between three carriers (2 ferredoxinred− + NAD(P)H + 3 H+ = 2 ferredoxinox + NAD(P)+ + 2 H2). A thermodynamic analysis of the bifurcating, [FeFe]-hydrogenase reaction, using el...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 9, 2019 Category: Biochemistry Source Type: research

Photochemical characterization of flavobacterial rhodopsin: The importance of the helix E region for heat stability
Publication date: Available online 6 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): SongI Han, Se-Hwan Kim, Jang-Chon Cho, Jaeho Song, Gwen Bleckner, Kwang-Hwan JungAbstractAlthough many microbial rhodopsins have been discovered many of organisms in a variety of habitats, little is known about the property and diversity of rhodopsin in flavobacteria. Recent studies discovered that many proteorhodopsin (PR)-like proteins exist in genomes of flavobacteria. Following the isolation of a flavobacterial rhodopsins (FR) from the flavobacteria IMCC1997 from the East Sea of Korea, we characterize...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 7, 2019 Category: Biochemistry Source Type: research

Role of the two PsaE isoforms on O2 reduction at photosystem I in Arabidopsis thaliana
Publication date: Available online 6 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Anja Krieger-Liszkay, Ginga Shimakawa, Pierre SétifAbstractLeaves of Arabidopsis thaliana plants grown in short days (8 h light) generate more reactive oxygen species in the light than leaves of plants grown in long days (16 h light). The importance of the two PsaE isoforms of photosystem I, PsaE1 and PsaE2, for O2 reduction was studied in plants grown under these different growth regimes. In short day conditions a mutant affected in the amount of PsaE1 (psae1-1) reduced more efficiently O2 tha...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 7, 2019 Category: Biochemistry Source Type: research

In the respiratory chain of Escherichia coli cytochromes bd-I and bd-II are more sensitive to carbon monoxide inhibition than cytochrome bo3
Publication date: Available online 4 November 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Elena Forte, Vitaliy B. Borisov, Sergey A. Siletsky, Maria Petrosino, Alessandro GiuffrèAbstractBacteria can not only encounter carbon monoxide (CO) in their habitats but also produce the gas endogenously. Bacterial respiratory oxidases, thus, represent possible targets for CO. Accordingly, host macrophages were proposed to produce CO and release it into the surrounding microenvironment to sense viable bacteria through a mechanism that in Escherichia (E.) coli was suggested to involve the targeting...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 5, 2019 Category: Biochemistry Source Type: research

Identification of assembly precursors to photosystems emitting fluorescence at 683 nm and 687 nm by cryogenic fluorescence microspectroscopy
Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Tomofumi Chiba, Yutaka ShibataAbstractPhotosystem I (PSI) and photosystem II (PSII) play key roles in photoinduced electron-transfer reaction in oxygenic photosynthesis. Assemblies of these PSs can be initiated by illumination of the etiolated seedlings (greening). The study aimed to identify specific fluorescence spectral components relevant to PSI and PSII assembly intermediates emerging in greening seedlings of Zea mays, a typical C4 plant. The different PSII contents between the bundle sheath (BS) and...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 1, 2019 Category: Biochemistry Source Type: research

Fourier transform infrared and mass spectrometry analyses of a site-directed mutant of D1-Asp170 as a ligand to the water-oxidizing Mn4CaO5 cluster in photosystem II
In this study, we constructed a D1-D170H mutant, in which the Asp ligand bridging Mn and Ca ions was replaced with His, in the cyanobacterium Synechocystis sp. PCC 6803, and analyzed isolated PSII core complexes using Fourier transform infrared (FTIR) difference spectroscopy and mass spectrometry (MS). The S2-minus-S1 FTIR difference spectrum of the PSII complexes of the D1-D170H mutant showed features virtually identical to those of the wild-type PSII. MS analysis further showed that ~70% of D1 proteins from the PSII complexes of D1-D170H possessed the wild-type amino acid sequence, although only the mutated sequence was ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 1, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 November 2019Source: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1860, Issue 11Author(s): (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - November 1, 2019 Category: Biochemistry Source Type: research

The BF4 and p71 antenna mutants from Chlamydomonas reinhardtii
Publication date: Available online 28 October 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Sandrine Bujaldon, Natsumi Kodama, Mithun Kumar Rathod, Nicolas Tourasse, Shin-Ichiro Ozawa, Julien Sellés, Olivier Vallon, Yuichiro Takahashi, Francis-André WollmanAbstractTwo pale green mutants of the green alga Chlamydomonas reinhardtii, which have been used over the years in many photosynthesis studies, the BF4 and p71 mutants, were characterized and their mutated gene identified in the nuclear genome. The BF4 mutant is defective in the insertase Alb3.1 whereas p71 is defective in cpSRP4...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - October 29, 2019 Category: Biochemistry Source Type: research

Remodeling of excitation energy transfer in extremophilic red algal PSI-LHCI complex during light adaptation
In this study we applied time-resolved fluorescence spectroscopy to characterize the kinetics of energy transfer processes in three types of PSI-LHCI supercomplexes isolated from the low (LL), medium (ML) and extreme high light (EHL) conditions. We show that the average rate of fluorescence decay is not correlated with the size of LHCI antenna and is twice faster in complexes isolated from ML-grown cells (~25–30 ps) than from both LL- and EHL-exposed cells (~50–55 ps). The difference is mainly due to a contribution of a long ~100-ps decay component detected only for the latter two PSI samples. We propose th...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - October 26, 2019 Category: Biochemistry Source Type: research

Does the water-oxidizing Mn4CaO5 cluster regulate the redox potential of the primary quinone electron acceptor QA in photosystem II? A study by Fourier transform infrared spectroelectrochemistry
In this study, we reinvestigated the effect of depletion of the Mn4CaO5 cluster on Em(QA−/QA) using Fourier transform infrared (FTIR) spectroelectrochemistry, which can directly monitor the redox state of QA at an intended potential. Light-induced FTIR difference measurements at a series of electrode potentials for intact and Mn-depleted PSII preparations from spinach and Thermosynechococcus elongatus showed that depletion of the Mn4CaO5 cluster hardly affected the Em(QA−/QA) values. In contrast, fluorescence spectroelectrochemical measurement using the same PSII sample, electrochemical cell, and redox mediator...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - October 26, 2019 Category: Biochemistry Source Type: research

An alternative plant-like cyanobacterial ferredoxin with unprecedented structural and functional properties: Ferredoxin with low Em discriminating against FNR
We report the structural and functional properties of a novel minor type ferredoxin, Fd2 of T. elongatus, homologous to Fed4 from Synechocystis sp. PCC 6803. Remarkably, the midpoint potential of Fd2, Em = −440 mV, is lower than that of Fd1, Em = −372 mV. However, while Fd2 can efficiently react with photosystem I or nitrite reductase, time-resolved spectroscopy shows that Fd2 has a very low capacity to reduce ferredoxin-NADP+ oxidoreductase (FNR). These unique Fd2 properties are discussed in relation with its structure, solved at 1.38 Å resolution. The Fd2 structure significantly differs fr...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - September 13, 2019 Category: Biochemistry Source Type: research

On the interface of light-harvesting antenna complexes and reaction centers in oxygenic photosynthesis
Publication date: Available online 10 September 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Haijun Liu, Robert E. BlankenshipAbstractPhotosynthetic pigment-protein complexes (PPCs) accomplish light-energy capture and photochemistry in natural photosynthesis. In this review, we examine three pigment protein complexes in oxygenic photosynthesis: light-harvesting antenna complexes and two reaction centers: Photosystem II (PSII), and Photosystem I (PSI). Recent technological developments promise unprecedented insights into how these multi–component protein complexes are assembled into higher...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - September 11, 2019 Category: Biochemistry Source Type: research

Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus
Publication date: Available online 11 September 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Andrea M. Lencina, Juergen Koepke, Julia Preu, Conny Muenke, Robert B. Gennis, Hartmut Michel, Lici A. Schurig-BriccioAbstractThe crystal structure of the enzyme previously characterized as a type-2 NADH:menaquinone oxidoreductase (NDH-2) from Thermus thermophilus has been solved at a resolution of 2.9 Å and revealed that this protein is, in fact, a coenzyme A-disulfide reductase (CoADR). Coenzyme A (CoASH) replaces glutathione as the major low molecular weight thiol in Thermus thermophilus and ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - September 11, 2019 Category: Biochemistry Source Type: research

Regulation of photosynthetic cyclic electron flow pathways by adenylate status in higher plant chloroplasts
Publication date: Available online 11 September 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Nicholas Fisher, Terry M. Bricker, David M. KramerAbstractCylic electron flow (CEF) around Photosystem I in photosynthetic eukaryotes is likely to be necessary to augment ATP production, rapidly- and precisely balancing the plastid ATP/NADPH energy budget to meet the demands of downstream metabolism. Many regulatory aspects of this process are unclear. Here we demonstrate that the higher plant plastid NADH/Fd:plastoquinone reductase (NDH) and proposed PGR5/PGRL1 ferredoxin:plastoquinone reductase (FQR) ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - September 11, 2019 Category: Biochemistry Source Type: research

Tuning antenna function through hydrogen bonds to chlorophyll a
We describe a molecular mechanism tuning the functional properties of chlorophyll a (Chl-a) molecules in photosynthetic antenna proteins. Light-harvesting complexes from photosystem II in higher plants – specifically LHCII purified with α- or β-dodecyl-maltoside, along with CP29 – were probed by low-temperature absorption and resonance Raman spectroscopies. We show that hydrogen bonding to the conjugated keto carbonyl group of protein-bound Chl-a tunes the energy of its Soret and Qy absorption transitions, inducing red-shifts that are proportional to the strength of the hydrogen bond involved. Chls-a...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 31, 2019 Category: Biochemistry Source Type: research

Photosynthetic regulation under fluctuating light in field-grown Cerasus cerasoides: A comparison of young and mature leaves
Publication date: Available online 29 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Ying-Jie Yang, Shi-Bao Zhang, Ji-Hua Wang, Wei HuangAbstractPhotosystem I (PSI) is a potential target of photoinhibition under fluctuating light. However, photosynthetic regulation under fluctuating light in field-grown plants is little known. Furthermore, it is unclear how young leaves protect PSI against fluctuating light under natural field conditions. In the present study, we examined chlorophyll fluorescence, P700 redox state and the electrochromic shift signal in the young and mature leaves of field-...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 31, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 October 2019Source: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1860, Issue 10Author(s): (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 31, 2019 Category: Biochemistry Source Type: research

Publisher's Note
Publication date: 1 October 2019Source: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1860, Issue 10Author(s): (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 31, 2019 Category: Biochemistry Source Type: research

Structural diffusion properties of two atypical Dps from the cyanobacterium Nostoc punctiforme disclose interactions with ferredoxins and DNA
We report NpDps-ferredoxin interactions by fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET) methods. Dynamic light scattering, size exclusion chromatography and native gel electrophoresis results show that NpDps4 forms a dodecamer at both pH 6.0 and pH 8.0, while NpDps5 forms a dodecamer only at pH 6.0. In addition, FCS data clearly reveal that the non-canonical NpDps5 interacts with DNA at pH 6.0. Our spectroscopic analysis shows that [FeS] centers of the three recombinantly expressed and isolated ferredoxins are properly incorporated and are consistent with their respe...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 14, 2019 Category: Biochemistry Source Type: research

Discriminating changes in intracellular NADH/NAD+ levels due to anoxicity and H2 supply in R. eutropha cells using the Frex fluorescence sensor
Publication date: Available online 13 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): S. Wilkening, F.-J. Schmitt, O. Lenz, I. Zebger, M. Horch, T. FriedrichAbstractThe hydrogen-oxidizing “Knallgas” bacterium Ralstonia eutropha can thrive in aerobic and anaerobic environments and readily switches between heterotrophic and autotrophic metabolism, making it an attractive host for biotechnological applications including the sustainable H2-driven production of hydrocarbons. The soluble hydrogenase (SH), one out of four different [NiFe]-hydrogenases in R. eutropha, mediates H2 oxidat...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 14, 2019 Category: Biochemistry Source Type: research

Extensive remodeling of the photosynthetic apparatus alters energy transfer among photosynthetic complexes when cyanobacteria acclimate to far-red light
This article is part of a Special Issue entitled Light harvesting, edited by Dr. Roberta Croce. (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 14, 2019 Category: Biochemistry Source Type: research

Towards the competent conformation for catalysis in the ferredoxin-NADP+ reductase from the Brucella ovis pathogen
Publication date: Available online 5 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Daniel Pérez-Amigot, Víctor Taleb, Sergio Boneta, Ernesto Anoz-Carbonell, María Sebastián, Adrián Velázquez-Campoy, Víctor Polo, Marta Martínez-Júlvez, Milagros MedinaAbstractBrucella ovis encodes a bacterial subclass 1 ferredoxin-NADP(H) reductase (BoFPR) that, by similarity with other FPRs, is expected either to deliver electrons from NADPH to the redox-based metabolism and/or to oxidize NADPH to regulate the soxRS regulon that protects bacter...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 7, 2019 Category: Biochemistry Source Type: research

Mechanism of protonation of the over-reduced Mn4CaO5 cluster in photosystem II
Publication date: Available online 5 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Keisuke Saito, Hiroshi IshikitaAbstractBased on characterization by X-ray absorption spectroscopy, it has been proposed that the Mn4CaO5 cluster in the crystal structure of the water-oxidizing enzyme, photosystem II (PSII), may represent an over-reduced form arising from reduction by the X-ray beam. Using a quantum mechanical/molecular mechanical approach, and assuming that all of the μ-oxo bridges are deprotonated in S1, we analyzed the reduction process of the Mn4CaO5 cluster. In the crystal structure,...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 7, 2019 Category: Biochemistry Source Type: research

Fate of oxygen species from O2 activation at dimetal cofactors in an oxidase enzyme revealed by 57Fe nuclear resonance X-ray scattering and quantum chemistry
Publication date: Available online 5 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Stefan Mebs, Vivek Srinivas, Ramona Kositzki, Julia J. Griese, Martin Högbom, Michael HaumannAbstractOxygen (O2) activation is a central challenge in chemistry and catalyzed at prototypic dimetal cofactors in biological enzymes with diverse functions. Analysis of intermediates is required to elucidate the reaction paths of reductive O2 cleavage. An oxidase protein from the bacterium Geobacillus kaustophilus, R2lox, was used for aerobic in-vitro reconstitution with only 57Fe(II) or Mn(II) plus 57Fe(II) ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 7, 2019 Category: Biochemistry Source Type: research

Functional analysis of coiled-coil domains of MCU in mitochondrial calcium uptake
Publication date: Available online 5 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Takenori Yamamoto, Mizune Ozono, Akira Watanabe, Kosuke Maeda, Atsushi Nara, Mei Hashida, Yusuke Ido, Yuka Hiroshima, Akiko Yamada, Hiroshi Terada, Yasuo ShinoharaAbstractThe mitochondrial calcium uniporter (MCU) complex is a highly-selective calcium channel. This complex consists of MCU, mitochondrial calcium uptake proteins (MICUs), MCU regulator 1 (MCUR1), essential MCU regulator element (EMRE), etc. MCU, which is the pore-forming subunit, has 2 highly conserved coiled-coil domains (CC1 and CC2); however...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 7, 2019 Category: Biochemistry Source Type: research

Successes & challenges in the atomistic modeling of light-harvesting and its photoregulation
Publication date: Available online 3 August 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Lorenzo Cupellini, Mattia Bondanza, Michele Nottoli, Benedetta MennucciAbstractLight-harvesting is a crucial step of photosynthesis. Its mechanisms and related energetics have been revealed by a combination of experimental investigations and theoretical modeling. The success of theoretical modeling is largely due to the application of atomistic descriptions combining quantum chemistry, classical models and molecular dynamics techniques. Besides the important achievements obtained so far, a complete and quan...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 4, 2019 Category: Biochemistry Source Type: research

Complexome analysis of the nitrite-dependent methanotroph Methylomirabilis lanthanidiphila
In conclusion, complexome profiling validated the expression and composition of enzymes proposed to be involved in the energy, methane and nitrogen metabolism of M. lanthanidiphila, thereby further corroborating the metabolically unique and environmentally relevant process of nitrite-dependent methane oxidation.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - August 1, 2019 Category: Biochemistry Source Type: research

The H channel is not a proton transfer path in yeast cytochrome c oxidase
Publication date: Available online 30 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Aapo Malkamäki, Brigitte Meunier, Marco Reidelbach, Peter R. Rich, Vivek SharmaAbstractCytochrome c oxidases (CcOs) in the respiratory chains of mitochondria and bacteria are primary consumers of molecular oxygen, converting it to water with the concomitant pumping of protons across the membrane to establish a proton electrochemical gradient. Despite a relatively well understood proton pumping mechanism of bacterial CcOs, the role of the H channel in mitochondrial forms of CcO remains debated. Here, we ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 31, 2019 Category: Biochemistry Source Type: research

The human uncoupling proteins 5 and 6 (UCP5/SLC25A14 and UCP6/SLC25A30) transport sulfur oxyanions, phosphate and dicarboxylates
Publication date: Available online 26 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Ruggiero Gorgoglione, Vito Porcelli, Antonella Santoro, Lucia Daddabbo, Angelo Vozza, Magnus Monné, Maria Antonietta Di Noia, Luigi Palmieri, Ferdinando Palmieri, Giuseppe FiermonteAbstractThe human genome encodes 53 members of the solute carrier family 25 (SLC25), also called the mitochondrial carrier family. In this work, two members of this family, UCP5 (BMCP1, brain mitochondrial carrier protein 1 encoded by SLC25A14) and UCP6 (KMCP1, kidney mitochondrial carrier protein 1 encoded by SLC25A30) hav...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 28, 2019 Category: Biochemistry Source Type: research

Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids
Publication date: Available online 22 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): A. Strašková, G. Steinbach, G. Konert, E. Kotabová, J. Komenda, M. Tichý, R. KaňaAbstractThylakoids are the place of the light-photosynthetic reactions. To gain maximal efficiency, these reactions are conditional to proper pigment-pigment and protein-protein interactions. In higher plants thylakoids, the interactions lead to a lateral asymmetry in localization of protein complexes (i.e. granal/stromal thylakoids) that have been defined as a domain-like structures characteristic b...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 24, 2019 Category: Biochemistry Source Type: research

Tryptophan 224 of the rat mitochondrial carnitine/acylcarnitine carrier is crucial for the antiport mechanism
Publication date: Available online 21 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Nicola Giangregorio, Annamaria Tonazzi, Lara Console, Mariella Pistillo, Vito Scalera, Cesare IndiveriAbstractThe mitochondrial carnitine/acylcarnitine carrier (CACT) catalyzes an antiport of carnitine and acylcarnitines and also a uniport reaction with a rate of about one tenth with respect to the antiport rate. The antiport process results from the coupling of the two uniport reactions in opposite directions. In this mechanism, the transition of the carrier from the outward open conformation to the inward ...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 22, 2019 Category: Biochemistry Source Type: research

D1:Glu244 and D1:Tyr246 of the bicarbonate-binding environment of Photosystem II moderate high light susceptibility and electron transfer through the quinone-Fe-acceptor complex
Publication date: Available online 20 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Jack A. Forsman, Imre Vass, Julian J. Eaton-RyeAbstractIn cyanobacteria, Glu-244 and Tyr-246 of the Photosystem II (PS II) D1 protein are hydrogen bonded to two water molecules that are part of a hydrogen-bond network between the bicarbonate ligand to a non-heme iron and the cytosol. Ala substitutions were introduced in Synechocystis sp. PCC 6803 to investigate the roles of these residues and the hydrogen-bond network on electron transfer between the primary plastoquinone acceptor, QA, and the secondary plas...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 21, 2019 Category: Biochemistry Source Type: research

Binding of ferredoxin NADP+ oxidoreductase (FNR) to plant photosystem I
Publication date: Available online 20 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Pini Marco, Tamar Elman, Iftach YacobyAbstractThe binding of FNR to PSI has been postulated long ago, however, a clear evidence is still missing. In this work, using isothermal titration calorimetry (ITC), we found that FNR binds to photosystem I with its light harvesting complex I (PSI-LHCI) from C. reinhardtii with a 1:1 stoichiometry, a Kd of ~0.8 μM and ∆H of −20.7 kcal/mol. Titrations at different temperatures were used to determine the heat capacity change, ∆CP, of the binding, through...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 21, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 August 2019Source: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1860, Issue 8Author(s): (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 21, 2019 Category: Biochemistry Source Type: research

Insights into the mechanisms and dynamics of energy transfer in plant light-harvesting complexes from two-dimensional electronic spectroscopy
This article is part of a Special Issue entitled Light harvesting, edited by Dr. Roberta Croce.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 19, 2019 Category: Biochemistry Source Type: research

Calculated vibrational properties of semiquinones in the A1 binding site in photosystem I
Publication date: Available online 12 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Leyla Rohani, Hiroki Makita, Andrew Levitz, Maged Henary, Gary HastingsAbstractTime-resolved (P700+A1− – P700A1) FTIR difference spectra have been obtained using photosystem I (PSI) particles with several different quinones incorporated into the A1 protein binding site. Difference spectra were obtained for PSI with unlabeled and 18O labeled phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone) and 2-methyl-1,4-naphthaquinone (2MNQ) incorporated, and for PSI with unlabeled 2,3-dimethyl-1,4-naphthoq...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 13, 2019 Category: Biochemistry Source Type: research

The amazing phycobilisome
Publication date: Available online 12 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Noam Adir, Shira Bar-Zvi, Dvir HarrisAbstractCyanobacteria and red-algae share a common light-harvesting complex which is different than all other complexes which serve as photosynthetic antennas – the Phycobilisome (PBS). The PBS is found attached to the stromal side of thylakoid membranes, filling up most of the gap between individual thylakoids. The PBS self assembles from similar homologous protein units that are soluble and contain conserved cysteine residues that covalently bind the light absorbi...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 12, 2019 Category: Biochemistry Source Type: research

Digitonin-sensitive LHCII enlarges the antenna of Photosystem I in stroma lamellae of Arabidopsis thaliana after far-red and blue-light treatment
Publication date: Available online 9 July 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Peter Bos, Anniek Oosterwijk, Rob Koehorst, Arjen Bader, John Philippi, Herbert van Amerongen, Emilie WientjesAbstractLight drives photosynthesis. In plants it is absorbed by light-harvesting antenna complexes associated with Photosystem I (PSI) and photosystem II (PSII). As PSI and PSII work in series, it is important that the excitation pressure on the two photosystems is balanced. When plants are exposed to illumination that overexcites PSII, a special pool of the major light-harvesting complex LHCII is ph...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 10, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 July 2019Source: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1860, Issue 7Author(s): (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - July 3, 2019 Category: Biochemistry Source Type: research

Mitochondrial respirasome works as a single unit and the cross-talk between complexes I, III2 and IV stimulates NADH dehydrogenase activity
Publication date: Available online 25 June 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Meztli Reyes-Galindo, Roselia Suarez, Mercedes Esparza-Perusquía, Jaime de Lira-Sánchez, J. Pablo Pardo, Federico Martínez, Oscar Flores-HerreraAbstractUstilago maydis is an aerobic basidiomycete that depends on oxidative phosphorylation for its ATP supply, pointing to the mitochondrion as a key player in its energy metabolism. Mitochondrial respiratory complexes I, III2, and IV occur in supramolecular structures named respirasome. In this work, we characterized the subunit composition a...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - June 27, 2019 Category: Biochemistry Source Type: research

Interaction between γC87 and γR242 residues participates in energy coupling between catalysis and proton translocation in Escherichia coli ATP synthase
This study should provide more information of residue interactions at the rotor and stator interface in order to further elucidate the energetic mechanism of ATP synthase. (Source: Biochimica et Biophysica Acta (BBA) Bioenergetics)
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - June 27, 2019 Category: Biochemistry Source Type: research

Single-molecule study of redox control involved in establishing the spinach plastocyanin-cytochrome b6f electron transfer complex
Publication date: Available online 24 June 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Guy E. Mayneord, Cvetelin Vasilev, Lorna A. Malone, David J.K. Swainsbury, C. Neil Hunter, Matthew P. JohnsonAbstractSmall diffusible redox proteins play a ubiquitous role in bioenergetic systems, facilitating electron transfer (ET) between membrane bound complexes. Sustaining high ET turnover rates requires that the association between extrinsic and membrane-bound partners is highly specific, yet also sufficiently weak to promote rapid post-ET separation. In oxygenic photosynthesis the small soluble electro...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - June 25, 2019 Category: Biochemistry Source Type: research

Mitochondrial cristae narrowing upon higher 2-oxoglutarate load
Publication date: Available online 25 June 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Andrea Dlasková, Tomáš Špaček, Hana Engstová, Jitka Špačková, Adam Schröfel, Blanka Holendová, Katarína Smolková, Lydie Plecitá-Hlavatá, Petr JežekAbstractHypoxia causes mitochondrial cristae widening, enabled by the ~20% degradation of Mic60/mitofilin, with concomitant clustering of the MICOS complex, reflecting the widening of crista junctions (outlets) (Plecitá-Hlavatá et al. FASEB J., 2016 30:1941&nd...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - June 25, 2019 Category: Biochemistry Source Type: research

Multiple pathways of charge recombination revealed by the temperature dependence of electron transfer kinetics in cyanobacterial photosystem I
Publication date: Available online 25 June 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Georgy Milanovsky, Oksana Gopta, Anastasia Petrova, Mahir Mamedov, Michael Gorka, Dmitry Cherepanov, John H. Golbeck, Alexey SemenovAbstractThe kinetics of charge recombination in Photosystem I P700-FA/FB complexes and P700-FX cores lacking the terminal iron‑sulfur clusters were studied over a temperatures range of 310 K to 4.2 K. Analysis of the charge recombination kinetics in this temperature range allowed the assignment of backward electron transfer from the different electron acceptors to P700+. T...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - June 25, 2019 Category: Biochemistry Source Type: research